Lsi04G007680 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G007680
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Alpha-galactosidase
Location	chr04: 7184965 .. 7188929 (-)
RNA-Seq Expression	Lsi04G007680
Synteny	Lsi04G007680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCGACTCACATGGGAAGGGTAATTTCGGAATATCAAAATAAAATCAGTCGGTCCACTACCAGTAGTAGTAGGTACAGAAGTCCATATAAATACTGACACTCCCTTGAACTGGCTTATCCACGAATCAGAATTTCAATTTTTACGCTGCAAGTACTGACGAAGACGAAGAACTGAAGCAATTCTTACAGAGAGAAAGAGTTCTGAAGTGTAAAAATGGAGCGATGGAGTTACTGCAGAGGAGCGGCAATGACGGTGTTCGTTTTAGTGTTCTCATCGGTAATGCTGGAGAGTGTTTCAGCCACTTCCAGAATGACGGAGATCGCCGCCGACGGTGATTTGCTGAGGCGGAATTTGCTTGCTAATGGCCTTGGAGTTACTCCTCCGATGGGGTGAGTTCTCCGATCTAATACAATCTGTACGGTTTTCTGTTTGTTTATGCATTTCCTCTGTTTTGATCTCTGCAGAAATGATGCCAGTTACTGAACTTAATTTACGAGTTGATTTGTTATTCCTTTTTAACCAATTAGATCTCTCTCTCTCTCTCTCTCAAACTTTTGATTATTATCCAGTGGAACATCTAGGAAAACAGTGCCCTATGAGATCTTCTTTATTTTGTTAATTCTTCTTTTGAACGAGAAAGGTTTGACGTTATTGGCGCACACTTCTCTAGTTTATTAGTTTATTCGTCTCTCTATCGGTCCTTTTCATCATTATAGTAGCAAGATGAGTTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTGACTCACTGTTTGGATAAATTAGGTTTTATTCTCTTCTATCCAATGGGAGGAACACTGTTAATGAAACTGTTCATATTGTAATTTTGAAATTTCATTGTTGTTGGACAGATGGAATAGTTGGAATCACTTTGCATGCAATATCAATGAGAAAATGATTAGAGAAACCGGTAAAATTTCACCGTTCAATATCTTTCAATTTACTTCATCACTCAACATTAAGTGATCATGAATGACAAAATGACCTGGTTCGACAGCTGATGCTCTGGTTTCAACTGGCCTTTCGAAGCTTGGATATGAATATGTCAATATCGGTATGGGCCAATCTCTTCTCTCTTTCTCTTATATTTCAAGATTCAAAAGGTAAATTATATGATCTTTGGCTTTTCTTCTTAGATGATTGCTGGGCTGAGATAGCTCGTGATGATAAGGTAATTTCTTGCCCTCGTTTTTCTTCTTCCTTCAGATTGCATGTAAACCGTTTGATTTTATGTCTCTGAGAGTTTTGATTCTTTTTTATATGATTTCAGGGTAATCTGGTGCCGAAGAATTCAACATTTCCATCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATTTATTCAGATGCTGGGTATGTAACAGTGATTTTCAAGTGCCTAACCGTAATCTAGATTTTATTTTTTCCATTTCATGTCTTAATTTTGAAAAGTTATAATTTATCTTTTATGTATATGTATTAAAAAATTTCAATGAATTTATTTTCTGTAATAATAATAATTTATGAATTTGGTAAGTTGTTATAAGGGCCCAGAGTATACATAGTGGATAGATTTTCAATTTTATGATACGAATATTTAATTTATTAAATTAAAATATGGGATAATTCCAACTTAAATGAATTTTACCTTTTCTGACATTAAGGAGACATTTATGATTTGTACGTGAAAGGTGAATTGAAAAATTTTAAAATATTTGGGACACATTTTTTTTTAAGACAAATTTAGTATGAAATTTCATTTTCAACCTTTTCAAATACCTTTGCATATTCTCTATATGTGAAGTTGATCTACTATTCAAGCATTAAATTATCAAGCAACTGTGCAGGTATTTTACTTGCAGCAAAACCATGCCCGGCTCTCTTGGCCATGAGGAGCAAGACGCCAAAACTTTTGCTTCATGGGTATGAACAACAGAAAAATTCTCATTACTAAGTTTAATTAAATCAAACTTTCTTCTTCCTCTTCTTCTTTTTCCTTTCCTTTTTATAATTCATCTTCTAGGTGAAAGATTGTTGATGCACATTGTTTTACTTGGCAGGGGATTGACTATTTGAAGTATGATAATTGTAACAATGGTAACATTAAGCCAACTGTTAGGTAATTTAATTTCTACAAACAGAAATCAATCAGTTAAATTCCCTTTTGAGTTAGTCATATGTGGTGGTTTGAAAGATGAAGCTGCAGCACGTTGATTATCTTTTCTTTTTCAGATATCCAGTCATGACTCGAGCGCTGATGAAGGCCGGACGCCCCATCTTCTTCTCTCTTTGTGAATGGTAATTACACCCTGCTTATCCCCAACTCAGGCTAGTCATAGGAAACTGTTCCTTGTGTATTTTATGGCATTCTCTCCCTTGCTTTGTTCTGCAGGGGAGACCTGCATCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATCAACGATTCGTGGGAGAGGTGGAATTTTTAAACGAAGTTTTGCTTAGTTGCTTATATTTTAACGCTTGAAATGGAAGGACTTGGGGGCATATTTGGGGAATGGTTTTGAAATTATTAAAACCACTCTTCTCGTATTCAAAATCACTCTAAAACACGAATTTAATCCTTCAAAATTAATTTTGCTGGCATGAAAATTGCGTTTAAAAGTGTAAACTTAAACATTGAAAAAATTATATGAAAATCACTTTTAATTCATTTATAAACTAATCATCTTCATTAAAATCACTCCTAAACATGCACTAAAATACTCAAGATTCCATTTGCAGTATGATATCTAGAGCAGACCTCAATGAAGTATATGCTGATCTCGCAAGGCCCGGTGGCTGGAACGGTAGCGTAGTCTTCATATTGAGCCAAATCATAACTCTTAGAATACCTTTTACCATATTTAATATTCATTCTTGCAAATTGTTTTCAGATCCTGACATGCTCGAGGTTGGTAATGGAGGGATGACTAAGGATGAGTACATTGTTCACTTTAGTCTTTGGGCCATTTCCAAGGTATTGTGCAGCAGCAAAAACTTCTATTCCTTCACTGATCTTTTGGGATATTTGATAATGTGGGATGATGTTTTAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACGAAGGAGATCATTACGAACAAAGAAGTCATTGCAGTTAACCAAGGTATAAAATAGATGATTATTACACTTCTTTTTGGTCATAGATGTTATTGCTTGCTTCTCAATCTCACCATCTTCGTTCGATAATTCCCGAACCAGATCCCCTCGGCGTCCAAGCCAAGAAGGTCAGAAGTGAAGGTGATTTAGAGGTACAATCCAATGTTGTGATAATACTATTGCAAAGAGCATGATGCTTTTGTTGCAAATCAGGTTAGAGAAAAGAAAAAATAAATATTTTGTTTACTGTTTGTGTTTCAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGGTTGTGTTGCTGAACCGAGGGCCATGGCGTAATGCGATTACCGCTCAATGGGACGACATCGGAATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGGTAATTTGCTAGTATCTCCAAGCTTCATGTATATCATTACATGTATTTATGATGTGAATATCTGATTGTGTGTGAAAATTTGCAGCATACAACCTTGAAGACTATTTTTGTGGCAAATTTGACGGCTACTGTGGATTCTCATGCCTGCAAACTATATATTTTGAAGCCAATTTCTTAAGGTTAAAAGACATTAATTCCACATGTGAACATGGTTTGTCACTTCAAGAAATAAGGTTCAAGAACATTTTTGTTCACTGCTTTATTTTGAAGCTCTCCAAGTTTGAGGAAAAAGAAATAAAATGTCAGCATTAATTACGTTTCGATAATTTGTTTGTTTCTGTTTTGACTCGTAATTAAATCAACT

mRNA sequence

ATTCGACTCACATGGGAAGGGTAATTTCGGAATATCAAAATAAAATCAGTCGGTCCACTACCAGTAGTAGTAGGTACAGAAGTCCATATAAATACTGACACTCCCTTGAACTGGCTTATCCACGAATCAGAATTTCAATTTTTACGCTGCAAGTACTGACGAAGACGAAGAACTGAAGCAATTCTTACAGAGAGAAAGAGTTCTGAAGTGTAAAAATGGAGCGATGGAGTTACTGCAGAGGAGCGGCAATGACGGTGTTCGTTTTAGTGTTCTCATCGGTAATGCTGGAGAGTGTTTCAGCCACTTCCAGAATGACGGAGATCGCCGCCGACGGTGATTTGCTGAGGCGGAATTTGCTTGCTAATGGCCTTGGAGTTACTCCTCCGATGGGATGGAATAGTTGGAATCACTTTGCATGCAATATCAATGAGAAAATGATTAGAGAAACCGCTGATGCTCTGGTTTCAACTGGCCTTTCGAAGCTTGGATATGAATATGTCAATATCGATGATTGCTGGGCTGAGATAGCTCGTGATGATAAGGGTAATCTGGTGCCGAAGAATTCAACATTTCCATCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATTTATTCAGATGCTGGGTATTTTACTTGCAGCAAAACCATGCCCGGCTCTCTTGGCCATGAGGAGCAAGACGCCAAAACTTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGTAACAATGGTAACATTAAGCCAACTGTTAGATATCCAGTCATGACTCGAGCGCTGATGAAGGCCGGACGCCCCATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCATCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATCAACGATTCGTGGGAGAGGCCCGGTGGCTGGAACGGTAGCGTAGTCTTCATATTGAGCCAAATCATAACTCTTAGAATACCTTTTACCATATTTAATATTCATTCTTGCAAATTGTTTTCAGATCCTGACATGCTCGAGGTTGGTAATGGAGGGATGACTAAGGATGAGTACATTGTTCACTTTAGTCTTTGGGCCATTTCCAAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACGAAGGAGATCATTACGAACAAAGAAGTCATTGCAGTTAACCAAGATCCCCTCGGCGTCCAAGCCAAGAAGGTCAGAAGTGAAGGTGATTTAGAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGGTTGTGTTGCTGAACCGAGGGCCATGGCGTAATGCGATTACCGCTCAATGGGACGACATCGGAATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGCATACAACCTTGAAGACTATTTTTGTGGCAAATTTGACGGCTACTGTGGATTCTCATGCCTGCAAACTATATATTTTGAAGCCAATTTCTTAAGGTTAAAAGACATTAATTCCACATGTGAACATGGTTTGTCACTTCAAGAAATAAGGTTCAAGAACATTTTTGTTCACTGCTTTATTTTGAAGCTCTCCAAGTTTGAGGAAAAAGAAATAAAATGTCAGCATTAATTACGTTTCGATAATTTGTTTGTTTCTGTTTTGACTCGTAATTAAATCAACT

Coding sequence (CDS)

ATGGAGCGATGGAGTTACTGCAGAGGAGCGGCAATGACGGTGTTCGTTTTAGTGTTCTCATCGGTAATGCTGGAGAGTGTTTCAGCCACTTCCAGAATGACGGAGATCGCCGCCGACGGTGATTTGCTGAGGCGGAATTTGCTTGCTAATGGCCTTGGAGTTACTCCTCCGATGGGATGGAATAGTTGGAATCACTTTGCATGCAATATCAATGAGAAAATGATTAGAGAAACCGCTGATGCTCTGGTTTCAACTGGCCTTTCGAAGCTTGGATATGAATATGTCAATATCGATGATTGCTGGGCTGAGATAGCTCGTGATGATAAGGGTAATCTGGTGCCGAAGAATTCAACATTTCCATCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATTTATTCAGATGCTGGGTATTTTACTTGCAGCAAAACCATGCCCGGCTCTCTTGGCCATGAGGAGCAAGACGCCAAAACTTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGTAACAATGGTAACATTAAGCCAACTGTTAGATATCCAGTCATGACTCGAGCGCTGATGAAGGCCGGACGCCCCATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCATCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATCAACGATTCGTGGGAGAGGCCCGGTGGCTGGAACGGTAGCGTAGTCTTCATATTGAGCCAAATCATAACTCTTAGAATACCTTTTACCATATTTAATATTCATTCTTGCAAATTGTTTTCAGATCCTGACATGCTCGAGGTTGGTAATGGAGGGATGACTAAGGATGAGTACATTGTTCACTTTAGTCTTTGGGCCATTTCCAAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACGAAGGAGATCATTACGAACAAAGAAGTCATTGCAGTTAACCAAGATCCCCTCGGCGTCCAAGCCAAGAAGGTCAGAAGTGAAGGTGATTTAGAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGGTTGTGTTGCTGAACCGAGGGCCATGGCGTAATGCGATTACCGCTCAATGGGACGACATCGGAATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGCATACAACCTTGAAGACTATTTTTGTGGCAAATTTGACGGCTACTGTGGATTCTCATGCCTGCAAACTATATATTTTGAAGCCAATTTCTTAA

Protein sequence

MERWSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWERPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS

Homology

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BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 3.4e-183
Identity = 302/430 (70.23%), Postives = 343/430 (79.77%), Query Frame = 0


Query: 12  MTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSWNHFACNIN 71
           M + +++ SS+++  V ++  +     D ++LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 72  EKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGMKALADYVH 131
           EKMI+ETADALV+TGLSKLGY YVNIDDCWAEI+RD KG+LVPK STFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 132 AKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYP 191
           +KGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPTVRYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 192 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW-------------- 251
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W              
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 252 --ERPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFS 311
              RPGGWN                             DPDMLEVGNGGMTKDEYIVHFS
Sbjct: 250 EHARPGGWN-----------------------------DPDMLEVGNGGMTKDEYIVHFS 309

Query: 312 LWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS 371
           +WAISKAPLLLGCD+RN+TKET EI+ NKEVIA+NQDP GVQAKKVR EGDLEVWAGPLS
Sbjct: 310 IWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLS 369

Query: 372 GYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHA 426
           GYRVA++LLNRGP R +ITA W+DI IP NS V+ARDLWEH TLK  FV NLTATVDSHA
Sbjct: 370 GYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHA 410

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BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 7.1e-149
Identity = 245/399 (61.40%), Postives = 293/399 (73.43%), Query Frame = 0


Query: 41  DLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDC 100
           D  RR+LLANGLG+TPPMGWNSWNHF CN++EK+IRETADA+VS GL+ LGY+Y+N+DDC
Sbjct: 9   DYTRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDC 68

Query: 101 WAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEE 160
           WAE+ RD +GNLVPK STFPSG+KALADYVH+KGLKLGIYSDAG  TCSKTMPGSLGHEE
Sbjct: 69  WAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEE 128

Query: 161 QDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWG 220
           QDAKTFASWG+DYLKYDNCNN NI P  RYP+M++AL+ +GR IFFSLCEWG+  PA W 
Sbjct: 129 QDAKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWA 188

Query: 221 DKLGNSWRTTNDINDSWER----------------PGGWNGSVVFILSQIITLRIPFTIF 280
            ++GNSWRTT DI+DSW                  PGGWN                    
Sbjct: 189 KEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWN-------------------- 248

Query: 281 NIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNK 340
                    DPDMLEVGNGGMT  EY  HFS+WA++KAPLL+GCD+R++   T ++++N 
Sbjct: 249 ---------DPDMLEVGNGGMTTTEYRSHFSIWALAKAPLLIGCDIRSMDGATFQLLSNA 308

Query: 341 EVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPP 400
           EVIAVNQD LGVQ  KV++ GDLEVWAGPLSG RVAV L NRG     ITA W D+G+P 
Sbjct: 309 EVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSGKRVAVALWNRGSSTATITAYWSDVGLPS 368

Query: 401 NSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKP 424
            + V ARDLW H+T K++    ++A VD+H  K+Y+L P
Sbjct: 369 TAVVNARDLWAHSTEKSV-KGQISAAVDAHDSKMYVLTP 377

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BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 8.4e-142
Identity = 241/438 (55.02%), Postives = 297/438 (67.81%), Query Frame = 0


Query: 4   WSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSW 63
           +S   G  + V +++  S     +   +R T   A+   +RR L  NGLG TPPMGWNSW
Sbjct: 5   YSIIGGMIIVVLLMIIGSEGGRLLEKKNR-TSAEAEHYNVRRYLAENGLGQTPPMGWNSW 64

Query: 64  NHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGM 123
           NHF C+INE ++RETADA+VSTGL+ LGY+Y+N+DDCWAE+ RD +GN+VP  + FPSG+
Sbjct: 65  NHFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGI 124

Query: 124 KALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGN 183
           KALADYVH+KGLKLG+YSDAG  TCSK MPGSLGHEEQDAKTFASWG+DYLKYDNC N  
Sbjct: 125 KALADYVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLG 184

Query: 184 IKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWER---- 243
           I    RYP M +AL+ +GRPIFFS+CEWG   P +W   +GNSWRTT DI D+W      
Sbjct: 185 ISVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSI 244

Query: 244 ------------PGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTK 303
                       PGGWN                             DPDMLEVGNGGMT 
Sbjct: 245 ADSNDKWASYAGPGGWN-----------------------------DPDMLEVGNGGMTT 304

Query: 304 DEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDL 363
           +EY  HFS+WA++KAPLL+GCD+R +   T E+I+N EVIAVNQD LGVQ KKV+S  DL
Sbjct: 305 EEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTNDL 364

Query: 364 EVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANL 423
           EVWAGPLS  +VAV+L NR   R  +TA W DIG+   + V ARDLWEH+T +++    +
Sbjct: 365 EVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHST-QSLVSGEI 411

Query: 424 TATVDSHACKLYILKPIS 426
           +A +DSHACK+Y+L P S
Sbjct: 425 SAEIDSHACKMYVLTPRS 411

[Close]

BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.1e-141
Identity = 244/422 (57.82%), Postives = 298/422 (70.62%), Query Frame = 0


Query: 16  VLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMI 75
           VL+  S+   + + T  +T+I ADG    R L+ NGL ++P MGWNSWNHF CNINE +I
Sbjct: 2   VLLSFSLRFIAFTLTITLTQI-ADG-FQSRMLMNNGLALSPQMGWNSWNHFQCNINETLI 61

Query: 76  RETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGL 135
           ++TADA+VS+GLS +GY+Y+NIDDCW E+ RD +G+LV K STFPSG+KAL+DYVH+KGL
Sbjct: 62  KQTADAMVSSGLSAIGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGL 121

Query: 136 KLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTR 195
           KLGIYSDAG  TCS+TMPGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++
Sbjct: 122 KLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSK 181

Query: 196 ALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE----------------R 255
           AL+ +GR IFFSLCEWG   PA W   +GNSWRTT DI D+W+                R
Sbjct: 182 ALLNSGRSIFFSLCEWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYAR 241

Query: 256 PGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAI 315
           PG WN                             DPDMLEVGNGGMTK+EY+ HFS+WA+
Sbjct: 242 PGSWN-----------------------------DPDMLEVGNGGMTKEEYMSHFSIWAL 301

Query: 316 SKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRV 375
           +KAPLL+GCDLR++ K T E+++NKEVIAVNQD LG+Q KKV+ EGDLEVWAGPLS  RV
Sbjct: 302 AKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRV 361

Query: 376 AVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLY 422
           AV+L NRG     ITA+W +IG+  +  V ARDLWEH+T   +    L+A V+ HACK+Y
Sbjct: 362 AVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KKQLSALVEPHACKMY 391

[Close]

BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 9.3e-133
Identity = 226/396 (57.07%), Postives = 271/396 (68.43%), Query Frame = 0


Query: 44  RRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAE 103
           RR    NGLG TP MGWNSWNHF C INE++IRETADALV+TGL+KLGY+YVNIDDCWAE
Sbjct: 52  RRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAE 111

Query: 104 IARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 163
            +RD +GN VP   TFPSG+KALADYVHAKGLKLGIYSDAG  TCS  MPGSL HEEQD 
Sbjct: 112 YSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDV 171

Query: 164 KTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKL 223
           KTFASWG+DYLKYDNCN+       RY  M+ A+   G+ IFFSLCEWG  +PA W  ++
Sbjct: 172 KTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRM 231

Query: 224 GNSWRTTNDINDSW----------------ERPGGWNGSVVFILSQIITLRIPFTIFNIH 283
           GNSWRTT DI D+W                  PGGWN                       
Sbjct: 232 GNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWN----------------------- 291

Query: 284 SCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVI 343
                 DPDMLEVGNGGM++ EY  HFS+WA++KAPLL+GCD+R+++++TK I++N EVI
Sbjct: 292 ------DPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVI 351

Query: 344 AVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSN 403
           AVNQD LGVQ KKV+S+  LEVWAGPLS  R AVVL NR  ++  ITA W +IG+  +  
Sbjct: 352 AVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVA 411

Query: 404 VQARDLWEHTTLKTIFVANLTATVDSHACKLYILKP 424
           V ARDLW H++        ++A+V  H CK+Y+L P
Sbjct: 412 VTARDLWAHSSFAA--QGQISASVAPHDCKMYVLTP 416

[Close]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL
Match: A0A5D3CXZ6 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003290 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.1e-236
Identity = 402/443 (90.74%), Postives = 408/443 (92.10%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLES-VSATSRMTE-IAADGDLLRRNLLANGLGVTPPM 60
           ME  SYC GAA+ VFVL FS VMLES VSATSR TE IA DGDLLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120
           GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 ----------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGN 300
                           RPGGWNGSV  ILSQIIT RI FTIF+IH CKLFSDPDMLEVGN
Sbjct: 241 SMISRADLNEIYADYARPGGWNGSVSLILSQIITPRISFTIFSIHFCKLFSDPDMLEVGN 300

Query: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVR 360
           GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVR
Sbjct: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVR 360

Query: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTI 420
           SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNV+ARDLWE TTLKT 
Sbjct: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVEARDLWERTTLKTT 420

Query: 421 FVANLTATVDSHACKLYILKPIS 426
           FVANLTATVDSHACKLYILKPIS
Sbjct: 421 FVANLTATVDSHACKLYILKPIS 443

[Close]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL
Match: Q2MK92 (Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.0e-219
Identity = 375/442 (84.84%), Postives = 385/442 (87.10%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLE-SVSATSRMTEIAADGDLLRRNLLANGLGVTPPMG 60
           ME  SYC+  A+ VFVL FS VMLE +VSATSRMTEIA+DGDLLRRNLLANGLGVTPPMG
Sbjct: 1   MECRSYCKAPAVVVFVLAFSLVMLETTVSATSRMTEIASDGDLLRRNLLANGLGVTPPMG 60

Query: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120
           WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF
Sbjct: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120

Query: 121 PSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNC 180
           PSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDNC
Sbjct: 121 PSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNC 180

Query: 181 NNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE- 240
           NNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 
Sbjct: 181 NNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240

Query: 241 ---------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNG 300
                          RPGGWN                             DPDMLEVGNG
Sbjct: 241 MISRADLNEIYADYARPGGWN-----------------------------DPDMLEVGNG 300

Query: 301 GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRS 360
           GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETK I+TN EVIAVNQDPLGVQAKKVRS
Sbjct: 301 GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKAIVTNTEVIAVNQDPLGVQAKKVRS 360

Query: 361 EGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIF 420
           EGDLEVWAGPLSGYRVAVVLLNRGPWRNAI+AQWDDIGIPPNSNV+ARDLWEHTTLKT F
Sbjct: 361 EGDLEVWAGPLSGYRVAVVLLNRGPWRNAISAQWDDIGIPPNSNVEARDLWEHTTLKTTF 413

Query: 421 VANLTATVDSHACKLYILKPIS 426
           VANLTATVDSHACKLYILKPIS
Sbjct: 421 VANLTATVDSHACKLYILKPIS 413

[Close]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL
Match: A0A1S3BDT4 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.9e-219
Identity = 379/443 (85.55%), Postives = 384/443 (86.68%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLES-VSATSRMTE-IAADGDLLRRNLLANGLGVTPPM 60
           ME  SYC GAA+ VFVL FS VMLES VSATSR TE IA DGDLLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120
           GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 ----------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGN 300
                           RPGGWN                             DPDMLEVGN
Sbjct: 241 SMISRADLNEIYADYARPGGWN-----------------------------DPDMLEVGN 300

Query: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVR 360
           GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVR
Sbjct: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVR 360

Query: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTI 420
           SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNV+ARDLWE TTLKT 
Sbjct: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVEARDLWERTTLKTT 414

Query: 421 FVANLTATVDSHACKLYILKPIS 426
           FVANLTATVDSHACKLYILKPIS
Sbjct: 421 FVANLTATVDSHACKLYILKPIS 414

[Close]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL
Match: A0A6J1HT03 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.1e-218
Identity = 373/441 (84.58%), Postives = 384/441 (87.07%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGW 60
           ME  SYC GAAM VFVLVFSSVM+ SV ATSR+ EI+A G+LLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP 120
           NSWNHFACNINE MI++TADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPK STFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE-- 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE  
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 --------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGG 300
                         RPGGWN                             DPDMLEVGNGG
Sbjct: 241 ISRADLNEPYADFARPGGWN-----------------------------DPDMLEVGNGG 300

Query: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSE 360
           MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLG+QAKKVRSE
Sbjct: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGIQAKKVRSE 360

Query: 361 GDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 420
           GDLEVWAGPLS YRVA+VLLNRGPWR++ITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV
Sbjct: 361 GDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 412

Query: 421 ANLTATVDSHACKLYILKPIS 426
           ANLTATV+SHACKLYILKPIS
Sbjct: 421 ANLTATVNSHACKLYILKPIS 412

[Close]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL
Match: A0A6J1G2A9 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.3e-218
Identity = 373/441 (84.58%), Postives = 383/441 (86.85%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGW 60
           ME  SYC GAAM VFV VFSSVM+ SV ATSR+ EI+A G+LLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMGVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP 120
           NSWNHFACNINE MI++TADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPK STFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE-- 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE  
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 --------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGG 300
                         RPGGWN                             DPDMLEVGNGG
Sbjct: 241 ISRADLNEPYADFARPGGWN-----------------------------DPDMLEVGNGG 300

Query: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSE 360
           MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVRSE
Sbjct: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSE 360

Query: 361 GDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 420
           GDLEVWAGPLS YRVA+VLLNRGPWR++ITAQWDDIGIPPNSNV+ARDLWEHTTLKTIFV
Sbjct: 361 GDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFV 412

Query: 421 ANLTATVDSHACKLYILKPIS 426
           ANLTATVDSHACKLYILKPIS
Sbjct: 421 ANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lsi04G007680 vs. NCBI nr
Match: KAA0034094.1 (alpha-galactosidase 1 [Cucumis melo var. makuwa] >TYK15824.1 alpha-galactosidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 827.8 bits (2137), Expect = 4.3e-236
Identity = 402/443 (90.74%), Postives = 408/443 (92.10%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLES-VSATSRMTE-IAADGDLLRRNLLANGLGVTPPM 60
           ME  SYC GAA+ VFVL FS VMLES VSATSR TE IA DGDLLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120
           GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 ----------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGN 300
                           RPGGWNGSV  ILSQIIT RI FTIF+IH CKLFSDPDMLEVGN
Sbjct: 241 SMISRADLNEIYADYARPGGWNGSVSLILSQIITPRISFTIFSIHFCKLFSDPDMLEVGN 300

Query: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVR 360
           GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVR
Sbjct: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVR 360

Query: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTI 420
           SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNV+ARDLWE TTLKT 
Sbjct: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVEARDLWERTTLKTT 420

Query: 421 FVANLTATVDSHACKLYILKPIS 426
           FVANLTATVDSHACKLYILKPIS
Sbjct: 421 FVANLTATVDSHACKLYILKPIS 443

[Close]

BLAST of Lsi04G007680 vs. NCBI nr
Match: XP_038891481.1 (alpha-galactosidase 1-like [Benincasa hispida])

HSP 1 Score: 786.2 bits (2029), Expect = 1.4e-223
Identity = 382/441 (86.62%), Postives = 386/441 (87.53%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGW 60
           ME  SYCRGAAM VFVL+FS VMLESVSATSRMTEIA DGDLLRRNLLANGLG TPPMGW
Sbjct: 1   MECRSYCRGAAMAVFVLLFSLVMLESVSATSRMTEIATDGDLLRRNLLANGLGATPPMGW 60

Query: 61  NSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP 120
           NSWNHFACNINEKMIRETADAL+STGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP
Sbjct: 61  NSWNHFACNINEKMIRETADALISTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAK FASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKIFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE-- 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE  
Sbjct: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 --------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGG 300
                         RPGGWN                             DPDMLEVGNGG
Sbjct: 241 ISRADLNEVYADLARPGGWN-----------------------------DPDMLEVGNGG 300

Query: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSE 360
           MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEII NKEVIAVNQDPLGVQAKKVRSE
Sbjct: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIIMNKEVIAVNQDPLGVQAKKVRSE 360

Query: 361 GDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 420
           GDLEVWAGPLSGYRVA+VLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKT FV
Sbjct: 361 GDLEVWAGPLSGYRVALVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTTFV 412

Query: 421 ANLTATVDSHACKLYILKPIS 426
           ANLTATV SHACKLYILKPIS
Sbjct: 421 ANLTATVASHACKLYILKPIS 412

[Close]

BLAST of Lsi04G007680 vs. NCBI nr
Match: NP_001267500.1 (alpha-galactosidase-like precursor [Cucumis sativus] >ABC55266.1 acid alpha galactosidase 1 [Cucumis sativus] >KAE8648624.1 hypothetical protein Csa_007924 [Cucumis sativus])

HSP 1 Score: 772.3 bits (1993), Expect = 2.1e-219
Identity = 375/442 (84.84%), Postives = 385/442 (87.10%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLE-SVSATSRMTEIAADGDLLRRNLLANGLGVTPPMG 60
           ME  SYC+  A+ VFVL FS VMLE +VSATSRMTEIA+DGDLLRRNLLANGLGVTPPMG
Sbjct: 1   MECRSYCKAPAVVVFVLAFSLVMLETTVSATSRMTEIASDGDLLRRNLLANGLGVTPPMG 60

Query: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120
           WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF
Sbjct: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120

Query: 121 PSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNC 180
           PSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDNC
Sbjct: 121 PSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNC 180

Query: 181 NNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE- 240
           NNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 
Sbjct: 181 NNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240

Query: 241 ---------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNG 300
                          RPGGWN                             DPDMLEVGNG
Sbjct: 241 MISRADLNEIYADYARPGGWN-----------------------------DPDMLEVGNG 300

Query: 301 GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRS 360
           GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETK I+TN EVIAVNQDPLGVQAKKVRS
Sbjct: 301 GMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKAIVTNTEVIAVNQDPLGVQAKKVRS 360

Query: 361 EGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIF 420
           EGDLEVWAGPLSGYRVAVVLLNRGPWRNAI+AQWDDIGIPPNSNV+ARDLWEHTTLKT F
Sbjct: 361 EGDLEVWAGPLSGYRVAVVLLNRGPWRNAISAQWDDIGIPPNSNVEARDLWEHTTLKTTF 413

Query: 421 VANLTATVDSHACKLYILKPIS 426
           VANLTATVDSHACKLYILKPIS
Sbjct: 421 VANLTATVDSHACKLYILKPIS 413

[Close]

BLAST of Lsi04G007680 vs. NCBI nr
Match: KAG6573765.1 (Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 771.2 bits (1990), Expect = 4.8e-219
Identity = 375/441 (85.03%), Postives = 384/441 (87.07%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGW 60
           ME  SYC GAAM VFVLVFSSVM+ SV ATSR+ EI+A G+LLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVMSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFP 120
           NSWNHFACNINE MI++TADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPK STFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE-- 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE  
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 --------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGG 300
                         RPGGWN                             DPDMLEVGNGG
Sbjct: 241 ISRADLNEPYADFARPGGWN-----------------------------DPDMLEVGNGG 300

Query: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSE 360
           MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVRSE
Sbjct: 301 MTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSE 360

Query: 361 GDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 420
           GDLEVWAGPLS YRVA+VLLNRGPWR++ITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV
Sbjct: 361 GDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNVQARDLWEHTTLKTIFV 412

Query: 421 ANLTATVDSHACKLYILKPIS 426
           ANLTATVDSHACKLYILKPIS
Sbjct: 421 ANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lsi04G007680 vs. NCBI nr
Match: XP_008445910.1 (PREDICTED: alpha-galactosidase 1 [Cucumis melo])

HSP 1 Score: 770.4 bits (1988), Expect = 8.1e-219
Identity = 379/443 (85.55%), Postives = 384/443 (86.68%), Query Frame = 0


Query: 1   MERWSYCRGAAMTVFVLVFSSVMLES-VSATSRMTE-IAADGDLLRRNLLANGLGVTPPM 60
           ME  SYC GAA+ VFVL FS VMLES VSATSR TE IA DGDLLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120
           GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 ----------------RPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGN 300
                           RPGGWN                             DPDMLEVGN
Sbjct: 241 SMISRADLNEIYADYARPGGWN-----------------------------DPDMLEVGN 300

Query: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVR 360
           GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITN EVIAVNQDPLGVQAKKVR
Sbjct: 301 GGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVR 360

Query: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTI 420
           SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNV+ARDLWE TTLKT 
Sbjct: 361 SEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVEARDLWERTTLKTT 414

Query: 421 FVANLTATVDSHACKLYILKPIS 426
           FVANLTATVDSHACKLYILKPIS
Sbjct: 421 FVANLTATVDSHACKLYILKPIS 414

[Close]

BLAST of Lsi04G007680 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 642.5 bits (1656), Expect = 2.4e-184
Identity = 302/430 (70.23%), Postives = 343/430 (79.77%), Query Frame = 0


Query: 12  MTVFVLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSWNHFACNIN 71
           M + +++ SS+++  V ++  +     D ++LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 72  EKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGMKALADYVH 131
           EKMI+ETADALV+TGLSKLGY YVNIDDCWAEI+RD KG+LVPK STFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 132 AKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYP 191
           +KGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPTVRYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 192 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW-------------- 251
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W              
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 252 --ERPGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFS 311
              RPGGWN                             DPDMLEVGNGGMTKDEYIVHFS
Sbjct: 250 EHARPGGWN-----------------------------DPDMLEVGNGGMTKDEYIVHFS 309

Query: 312 LWAISKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS 371
           +WAISKAPLLLGCD+RN+TKET EI+ NKEVIA+NQDP GVQAKKVR EGDLEVWAGPLS
Sbjct: 310 IWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLS 369

Query: 372 GYRVAVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHA 426
           GYRVA++LLNRGP R +ITA W+DI IP NS V+ARDLWEH TLK  FV NLTATVDSHA
Sbjct: 370 GYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHA 410

[Close]

BLAST of Lsi04G007680 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 504.6 bits (1298), Expect = 7.8e-143
Identity = 244/422 (57.82%), Postives = 298/422 (70.62%), Query Frame = 0


Query: 16  VLVFSSVMLESVSATSRMTEIAADGDLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMI 75
           VL+  S+   + + T  +T+I ADG    R L+ NGL ++P MGWNSWNHF CNINE +I
Sbjct: 2   VLLSFSLRFIAFTLTITLTQI-ADG-FQSRMLMNNGLALSPQMGWNSWNHFQCNINETLI 61

Query: 76  RETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGL 135
           ++TADA+VS+GLS +GY+Y+NIDDCW E+ RD +G+LV K STFPSG+KAL+DYVH+KGL
Sbjct: 62  KQTADAMVSSGLSAIGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGL 121

Query: 136 KLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTR 195
           KLGIYSDAG  TCS+TMPGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++
Sbjct: 122 KLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSK 181

Query: 196 ALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE----------------R 255
           AL+ +GR IFFSLCEWG   PA W   +GNSWRTT DI D+W+                R
Sbjct: 182 ALLNSGRSIFFSLCEWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYAR 241

Query: 256 PGGWNGSVVFILSQIITLRIPFTIFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAI 315
           PG WN                             DPDMLEVGNGGMTK+EY+ HFS+WA+
Sbjct: 242 PGSWN-----------------------------DPDMLEVGNGGMTKEEYMSHFSIWAL 301

Query: 316 SKAPLLLGCDLRNLTKETKEIITNKEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRV 375
           +KAPLL+GCDLR++ K T E+++NKEVIAVNQD LG+Q KKV+ EGDLEVWAGPLS  RV
Sbjct: 302 AKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRV 361

Query: 376 AVVLLNRGPWRNAITAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLY 422
           AV+L NRG     ITA+W +IG+  +  V ARDLWEH+T   +    L+A V+ HACK+Y
Sbjct: 362 AVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KKQLSALVEPHACKMY 391

[Close]

BLAST of Lsi04G007680 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 502.7 bits (1293), Expect = 3.0e-142
Identity = 235/395 (59.49%), Postives = 284/395 (71.90%), Query Frame = 0


Query: 43  LRRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWA 102
           + R L+ NGL ++P MGWNSWNHF CNINE +I++TADA+VS+GLS +GY+Y+NIDDCW 
Sbjct: 1   MSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG 60

Query: 103 EIARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQD 162
           E+ RD +G+LV K STFPSG+KAL+DYVH+KGLKLGIYSDAG  TCS+TMPGSLGHEEQD
Sbjct: 61  ELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQD 120

Query: 163 AKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDK 222
           AKTFASWGIDYLKYDNC N    P  RYP M++AL+ +GR IFFSLCEWG   PA W   
Sbjct: 121 AKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGD 180

Query: 223 LGNSWRTTNDINDSWE----------------RPGGWNGSVVFILSQIITLRIPFTIFNI 282
           +GNSWRTT DI D+W+                RPG WN                      
Sbjct: 181 IGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWN---------------------- 240

Query: 283 HSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNKEV 342
                  DPDMLEVGNGGMTK+EY+ HFS+WA++KAPLL+GCDLR++ K T E+++NKEV
Sbjct: 241 -------DPDMLEVGNGGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEV 300

Query: 343 IAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDDIGIPPNS 402
           IAVNQD LG+Q KKV+ EGDLEVWAGPLS  RVAV+L NRG     ITA+W +IG+  + 
Sbjct: 301 IAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSD 360

Query: 403 NVQARDLWEHTTLKTIFVANLTATVDSHACKLYIL 422
            V ARDLWEH+T   +    L+A V+ HACK+Y L
Sbjct: 361 IVNARDLWEHSTYSCV-KKQLSALVEPHACKMYTL 365

[Close]

BLAST of Lsi04G007680 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.1e-127
Identity = 221/404 (54.70%), Postives = 265/404 (65.59%), Query Frame = 0


Query: 39  DGDLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNID 98
           D  +  R  L NGL  TP MGWNSWN FACNINE +I+ETADALVS+GL+ LGY +VNID
Sbjct: 56  DTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNID 115

Query: 99  DCWAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGH 158
           DCW+ + RD +G LVP   TFPSG+K LADYVH+KGLKLGIYSDAG FTC +  PGSL H
Sbjct: 116 DCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFH 175

Query: 159 EEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPAL 218
           E  DA  FASWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PAL
Sbjct: 176 EVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPAL 235

Query: 219 WGDKLGNSWRTTNDINDSWER----------------PGGWNGSVVFILSQIITLRIPFT 278
           W  ++GNSWRTT+DIND+W                  PGGWN                  
Sbjct: 236 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWN------------------ 295

Query: 279 IFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIIT 338
                      DPDMLE+GNGGMT +EY  HFS+WA+ KAPLL+GCD+RN+T ET EI++
Sbjct: 296 -----------DPDMLEIGNGGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILS 355

Query: 339 NKEVIAVNQDPLGVQAKKVRSEGD---LEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDD 398
           NKE+IAVNQDPLGVQ +K+++ G+    +VW+GPLSG R+ V L NR      ITA WD 
Sbjct: 356 NKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDM 415

Query: 399 IGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKP 424
           IG+    +V  RDLW+H  +      +  A VD+H C +Y+L P
Sbjct: 416 IGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTP 429

[Close]

BLAST of Lsi04G007680 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 405.2 bits (1040), Expect = 6.4e-113
Identity = 207/404 (51.24%), Postives = 248/404 (61.39%), Query Frame = 0


Query: 39  DGDLLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNID 98
           D  +  R  L NGL  TP MGWNSWN FACNINE +I+ETADALVS+GL+ LGY +VNI 
Sbjct: 56  DTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI- 115

Query: 99  DCWAEIARDDKGNLVPKNSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGH 158
                                  G+K LADYVH+KGLKLGIYSDAG FTC +  PGSL H
Sbjct: 116 -----------------------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFH 175

Query: 159 EEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPAL 218
           E  DA  FASWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PAL
Sbjct: 176 EVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPAL 235

Query: 219 WGDKLGNSWRTTNDINDSWER----------------PGGWNGSVVFILSQIITLRIPFT 278
           W  ++GNSWRTT+DIND+W                  PGGWN                  
Sbjct: 236 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWN------------------ 295

Query: 279 IFNIHSCKLFSDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIIT 338
                      DPDMLE+GNGGMT +EY  HFS+WA+ KAPLL+GCD+RN+T ET EI++
Sbjct: 296 -----------DPDMLEIGNGGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILS 355

Query: 339 NKEVIAVNQDPLGVQAKKVRSEGD---LEVWAGPLSGYRVAVVLLNRGPWRNAITAQWDD 398
           NKE+IAVNQDPLGVQ +K+++ G+    +VW+GPLSG R+ V L NR      ITA WD 
Sbjct: 356 NKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDM 405

Query: 399 IGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKP 424
           IG+    +V  RDLW+H  +      +  A VD+H C +Y+L P
Sbjct: 416 IGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTP 405

The following BLAST results are available for this feature:

BLAST of Lsi04G007680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FT97	3.4e-183	70.23	Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1	[more]
Q42656	7.1e-149	61.40	Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1	[more]
P14749	8.4e-142	55.02	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]
Q8RX86	1.1e-141	57.82	Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1	[more]
Q9FXT4	9.3e-133	57.07	Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=...	[more]

BLAST of Lsi04G007680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CXZ6	2.1e-236	90.74	Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0...	[more]
Q2MK92	1.0e-219	84.84	Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A1S3BDT4	3.9e-219	85.55	Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1	[more]
A0A6J1HT03	1.1e-218	84.58	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1	[more]
A0A6J1G2A9	3.3e-218	84.58	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1	[more]

BLAST of Lsi04G007680 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0034094.1	4.3e-236	90.74	alpha-galactosidase 1 [Cucumis melo var. makuwa] >TYK15824.1 alpha-galactosidase...	[more]
XP_038891481.1	1.4e-223	86.62	alpha-galactosidase 1-like [Benincasa hispida]	[more]
NP_001267500.1	2.1e-219	84.84	alpha-galactosidase-like precursor [Cucumis sativus] >ABC55266.1 acid alpha gala...	[more]
KAG6573765.1	4.8e-219	85.03	Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_008445910.1	8.1e-219	85.55	PREDICTED: alpha-galactosidase 1 [Cucumis melo]	[more]

BLAST of Lsi04G007680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08380.1	2.4e-184	70.23	alpha-galactosidase 1	[more]
AT5G08370.1	7.8e-143	57.82	alpha-galactosidase 2	[more]
AT5G08370.2	3.0e-142	59.49	alpha-galactosidase 2	[more]
AT3G56310.1	2.1e-127	54.70	Melibiase family protein	[more]
AT3G56310.2	6.4e-113	51.24	Melibiase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PRINTS	PR00740	GLHYDRLASE27	coord: 189..207 score: 55.79 coord: 86..101 score: 65.94 coord: 127..148 score: 62.27 coord: 162..179 score: 76.39 coord: 267..286 score: 46.25 coord: 50..69 score: 69.5 coord: 288..309 score: 52.27
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 55..332 e-value: 2.4E-74 score: 250.0
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 12..424
IPR002241	Glycoside hydrolase, family 27	CDD	cd14792	GH27	coord: 56..332 e-value: 1.25949E-149 score: 423.505
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 335..424 e-value: 9.6E-28 score: 98.0
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 48..334 e-value: 6.6E-123 score: 411.6
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 344..421 e-value: 4.0E-19 score: 68.4
None	No IPR available	PANTHER	PTHR11452:SF82	ALPHA-GALACTOSIDASE	coord: 12..424
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 327..421
IPR000111	Glycoside hydrolase family 27/36, conserved site	PROSITE	PS00512	ALPHA_GALACTOSIDASE	coord: 91..107
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 49..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007680.1	Lsi04G007680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0052692	raffinose alpha-galactosidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds