Lsi04G007660 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G007660 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.3e-148
Identity = 268/419 (63.96%), Postives = 303/419 (72.32%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+ D +   
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG-- 88

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       + +++ QG+LVAKASTFPSGIKAL+DYVH KG
Sbjct: 89  ----------------------------ELKRDSQGSLVAKASTFPSGIKALSDYVHSKG 148

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAG  TCS+ MPGSLGHEEQDAKTFASWG                  RYPKMS
Sbjct: 149 LKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMS 208

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KAL  +GR I FSLCEW                         GQEDPATWA ++GNSWRT
Sbjct: 209 KALLNSGRSIFFSLCEW-------------------------GQEDPATWAGDIGNSWRT 268

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           T DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIWALAKA
Sbjct: 269 TGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALAKA 328

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS KRVAV+
Sbjct: 329 PLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVI 388

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSS-QVVQHQLTAQVDSHDCKMYVLT 462
           LWNRG   ANITA W++IGL++S  V ARD+WEHS+   V+ QL+A V+ H CKMY LT
Sbjct: 389 LWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKMYTLT 392

BLAST of Lsi04G007660 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.2e-148
Identity = 272/422 (64.45%), Postives = 296/422 (70.14%), Query Frame = 0

Query: 59  TRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYN 118
           TRR LL NGL LTPPMGWNSWNHF+CN++E LI+ETADAMVS GLAALGY+YINL D + 
Sbjct: 11  TRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCW- 70

Query: 119 SSLYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHR 178
                                         +  ++ QGNLV K STFPSGIKALADYVH 
Sbjct: 71  -----------------------------AELNRDSQGNLVPKGSTFPSGIKALADYVHS 130

Query: 179 KGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPK 238
           KGLKLGIYSDAG QTCSK MPGSLGHEEQDAKTFASWG                  RYP 
Sbjct: 131 KGLKLGIYSDAGTQTCSKTMPGSLGHEEQDAKTFASWGVDYLKYDNCNNNNISPKERYPI 190

Query: 239 MSKALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSW 298
           MSKAL  +GR I FSLCEW                         G+EDPATWA  VGNSW
Sbjct: 191 MSKALLNSGRSIFFSLCEW-------------------------GEEDPATWAKEVGNSW 250

Query: 299 RTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALA 358
           RTT DI D+W SMT+ AD NDKWASYA PGGWNDPDMLEVGNGGMTT EYRSHFSIWALA
Sbjct: 251 RTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSIWALA 310

Query: 359 KAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVA 418
           KAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG KV  YGDLEVWAGPLSGKRVA
Sbjct: 311 KAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSGKRVA 370

Query: 419 VVLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSSQ-VVQHQLTAQVDSHDCKMYVL 463
           V LWNRG   A ITA WSD+GL ++  V ARD+W HS++  V+ Q++A VD+HD KMYVL
Sbjct: 371 VALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAHDSKMYVL 377

BLAST of Lsi04G007660 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.5e-147
Identity = 266/460 (57.83%), Postives = 312/460 (67.83%), Query Frame = 0

Query: 21  VLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGLALTPPMGWNSWN 80
           ++  + ++GS G        G    +  R +AE +  + RR L  NGL  TPPMGWNSWN
Sbjct: 13  IVVLLMIIGSEG--------GRLLEKKNRTSAEAEHYNVRRYLAENGLGQTPPMGWNSWN 72

Query: 81  HFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIVGLNLTETLRMSM 140
           HF C+INEN+++ETADAMVS+GLAALGY+YINL D +                       
Sbjct: 73  HFGCDINENVVRETADAMVSTGLAALGYQYINLDDCW----------------------- 132

Query: 141 KISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPG 200
                   +  ++ +GN+V  A+ FPSGIKALADYVH KGLKLG+YSDAG QTCSK+MPG
Sbjct: 133 -------AELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRMPG 192

Query: 201 SLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGRPILFSLCEWLAI 260
           SLGHEEQDAKTFASWG                  RYP M KAL  +GRPI FS+CEW   
Sbjct: 193 SLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEW--- 252

Query: 261 FIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNWISMTTIADQNDK 320
                                 G EDP  WA ++GNSWRTT DI+DNW SMT+IAD NDK
Sbjct: 253 ----------------------GWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDK 312

Query: 321 WASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLS 380
           WASYA PGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+S
Sbjct: 313 WASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELIS 372

Query: 381 NKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGL 440
           N EVIAVNQDKLGVQGKKV    DLEVWAGPLS  +VAV+LWNR   RA +TASWSDIGL
Sbjct: 373 NAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGL 409

Query: 441 STSTTVTARDVWEHSSQ-VVQHQLTAQVDSHDCKMYVLTP 463
              TTV ARD+WEHS+Q +V  +++A++DSH CKMYVLTP
Sbjct: 433 QQGTTVDARDLWEHSTQSLVSGEISAEIDSHACKMYVLTP 409

BLAST of Lsi04G007660 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 5.9e-133
Identity = 258/472 (54.66%), Postives = 296/472 (62.71%), Query Frame = 0

Query: 9   SSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGL 68
           SSPPS  + L  ++     L    +  G+  A    SR     A + S   RR    NGL
Sbjct: 8   SSPPSPRLLLLLLVAVAATLLPEAAALGNFTA---ESR----GARWRSRRARRRAFENGL 67

Query: 69  ALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIV 128
             TP MGWNSWNHF C INE +I+ETADA+V++GLA LGY+Y+N+ D +           
Sbjct: 68  GRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCW----------- 127

Query: 129 GLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSD 188
                              E SR + QGN V    TFPSGIKALADYVH KGLKLGIYSD
Sbjct: 128 ------------------AEYSRDS-QGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSD 187

Query: 189 AGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGR 248
           AG QTCS KMPGSL HEEQD KTFASWG                  RY +MS A++  G+
Sbjct: 188 AGSQTCSNKMPGSLDHEEQDVKTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGK 247

Query: 249 PILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNW 308
            I FSLCEW                         G+E+PATWA  +GNSWRTT DI DNW
Sbjct: 248 NIFFSLCEW-------------------------GKENPATWAGRMGNSWRTTGDIADNW 307

Query: 309 ISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDI 368
            SMT+ AD+ND+WA+YA PGGWNDPDMLEVGNGGM+  EYRSHFSIWALAKAPLLIGCD+
Sbjct: 308 GSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIGCDV 367

Query: 369 RSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR 428
           RSM   T  +LSN EVIAVNQD LGVQGKKV     LEVWAGPLS  R AVVLWNR  ++
Sbjct: 368 RSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVLWNRQSYQ 417

Query: 429 ANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           A ITA WS+IGL+ S  VTARD+W HSS   Q Q++A V  HDCKMYVLTP+
Sbjct: 428 ATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQISASVAPHDCKMYVLTPN 417

BLAST of Lsi04G007660 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.3e-124
Identity = 233/427 (54.57%), Postives = 279/427 (65.34%), Query Frame = 0

Query: 55  DSASTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLG 114
           DS   RR LL NGL +TPPMGWNSWNHF CNI+E +IKETADA+V++GL+ LGY Y+N+ 
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DDYNSSLYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALAD 174
           D +                              E SR + +G+LV K STFPSGIKA+AD
Sbjct: 97  DCW-----------------------------AEISRDS-KGSLVPKKSTFPSGIKAVAD 156

Query: 175 YVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWG-----------------Y 234
           YVH KGLKLGIYSDAG  TCSK MPGSLG+EE DAKTFA WG                  
Sbjct: 157 YVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTV 216

Query: 235 RYPKMSKALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNV 294
           RYP M++AL ++GRPI  SLCEW                         G   PA W   V
Sbjct: 217 RYPVMTRALMKSGRPIFHSLCEW-------------------------GDMHPALWGSPV 276

Query: 295 GNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSI 354
           GNSWRTT+DI+D W+SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY  HFSI
Sbjct: 277 GNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSI 336

Query: 355 WALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSG 414
           WA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWAGPLSG
Sbjct: 337 WAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSG 396

Query: 415 KRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSS--QVVQHQLTAQVDSHDC 463
            RVA++L NRG  R +ITA W DI +  ++ V ARD+WEH +  Q     LTA VDSH C
Sbjct: 397 YRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHAC 408

BLAST of Lsi04G007660 vs. ExPASy TrEMBL
Match: A0A1S3BEH7 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.9e-199
Identity = 361/471 (76.65%), Postives = 378/471 (80.25%), Query Frame = 0

Query: 10  SPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGLA 69
           S PSVMV LY VL W FLLG  G+GNG AVAGA+RS  LRFAAEFDS S+RRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIVG 129
           LTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINL D +            
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCW------------ 125

Query: 130 LNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 189
                              +  ++ +GNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA
Sbjct: 126 ------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 185

Query: 190 GIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGRP 249
           GIQTCSKKMPGSLG+EEQDAKTFASWG                  RYPKM+KALQQ+GRP
Sbjct: 186 GIQTCSKKMPGSLGNEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRP 245

Query: 250 ILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNWI 309
           ILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDNWI
Sbjct: 246 ILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDNWI 305

Query: 310 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDIR 369
           SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCDIR
Sbjct: 306 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIR 365

Query: 370 SMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA 429
           SMDNIT+KLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA
Sbjct: 366 SMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA 419

Query: 430 NITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           NITASWSDIGL +STTVTARD+W+HSSQVVQH LTAQVDSHDCKM+VLTPH
Sbjct: 426 NITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 419

BLAST of Lsi04G007660 vs. ExPASy TrEMBL
Match: A0A5A7SVZ2 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003280 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 7.1e-198
Identity = 361/472 (76.48%), Postives = 378/472 (80.08%), Query Frame = 0

Query: 10  SPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGLA 69
           S PSVMV LY VL W FLLG  G+GNG AVAGA+RS  LRFAAEFDS S+RRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIVG 129
           LTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINL D +            
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCW------------ 125

Query: 130 LNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 189
                              +  ++ +GNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA
Sbjct: 126 ------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 185

Query: 190 GIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGRP 249
           GIQTCSKKMPGSLG+EEQDAKTFASWG                  RYPKM+KALQQ+GRP
Sbjct: 186 GIQTCSKKMPGSLGNEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRP 245

Query: 250 ILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNWI 309
           ILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDNWI
Sbjct: 246 ILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDNWI 305

Query: 310 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDIR 369
           SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCDIR
Sbjct: 306 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIR 365

Query: 370 SMDNITLKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR 429
           SMDNIT+KLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR
Sbjct: 366 SMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR 420

Query: 430 ANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           ANITASWSDIGL +STTVTARD+W+HSSQVVQH LTAQVDSHDCKM+VLTPH
Sbjct: 426 ANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 420

BLAST of Lsi04G007660 vs. ExPASy TrEMBL
Match: Q2HYY3 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.9e-196
Identity = 354/480 (73.75%), Postives = 378/480 (78.75%), Query Frame = 0

Query: 1   MAFPPLPLSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTR 60
           MA PP    S PSVM+ LY +LFW FLLG NG+GNG  VA A+RS  LRFA EFDSAS+R
Sbjct: 1   MALPP----SSPSVMLLLYFLLFWTFLLGGNGNGNGVVVAAASRSTALRFATEFDSASSR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           R+LLNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMVS+GLAALGY+YINL D +   
Sbjct: 61  RILLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCW--- 120

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       +  ++ +GNLVAKASTFPSGIKALADYVHRKG
Sbjct: 121 ---------------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKG 180

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAGI+TCSK+MPGSLGHEEQDAKTFASWG                  RYPKM+
Sbjct: 181 LKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMT 240

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KALQQ+GRPILFSLCEW                         GQEDPATWAVNVGNSWRT
Sbjct: 241 KALQQSGRPILFSLCEW-------------------------GQEDPATWAVNVGNSWRT 300

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMT  EYRSHFSIWALAKA
Sbjct: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWALAKA 360

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCDIRSMDN T+KLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAG LSGKRVAVV
Sbjct: 361 PLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRVAVV 420

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           LWNR LWRANITA WSDIGLS+STTVTARD+WEHSSQVV+H LTAQVDSHDCKM+VLTPH
Sbjct: 421 LWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVLTPH 421

BLAST of Lsi04G007660 vs. ExPASy TrEMBL
Match: A0A6J1FZL3 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.5e-195
Identity = 356/473 (75.26%), Postives = 372/473 (78.65%), Query Frame = 0

Query: 8   LSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNG 67
           +SSP S+M+ LYSVLFW FL  SN  GNG A+AGATRSRPLRFAAEFD  S RRVLLNNG
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSSN--GNGGAMAGATRSRPLRFAAEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMI 127
           LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINL D +          
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCW---------- 121

Query: 128 VGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYS 187
                                +  ++ +GNLV K+STFPSGIKALADYVHRKGLKLGIYS
Sbjct: 122 --------------------AELNRDAKGNLVPKSSTFPSGIKALADYVHRKGLKLGIYS 181

Query: 188 DAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAG 247
           DAGIQTCSKKMPGSLGHEEQDAKTFASWG                  RYPKM+KALQQ+G
Sbjct: 182 DAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSG 241

Query: 248 RPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDN 307
           RPILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDN
Sbjct: 242 RPILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDN 301

Query: 308 WISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCD 367
           W SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCD
Sbjct: 302 WNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCD 361

Query: 368 IRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLW 427
           IRSMDNITLKLLSNK VIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLWNRG +
Sbjct: 362 IRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLWNRGFY 417

Query: 428 RANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           RA ITASWS IGLS STTVTARD+WEHSSQVVQHQ+TAQVD HDCKMYVLTPH
Sbjct: 422 RAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

BLAST of Lsi04G007660 vs. ExPASy TrEMBL
Match: A0A6J1HTR9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 7.4e-195
Identity = 355/473 (75.05%), Postives = 370/473 (78.22%), Query Frame = 0

Query: 8   LSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNG 67
           +SSP S+M+ LYSVLFW F   SN  GNG A+AGATRSRPLRFA EFD  S RRVLLNNG
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSSN--GNGGAMAGATRSRPLRFATEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMI 127
           LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINL D +          
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCW---------- 121

Query: 128 VGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYS 187
                                +  ++ +GNLV K+STFPSGIKALADYVHRKGLKLGIYS
Sbjct: 122 --------------------AELNRDAKGNLVPKSSTFPSGIKALADYVHRKGLKLGIYS 181

Query: 188 DAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAG 247
           DAGIQTCSKKMPGSL HEEQDAKTFASWG                  RYPKM+KALQQ+G
Sbjct: 182 DAGIQTCSKKMPGSLDHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSG 241

Query: 248 RPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDN 307
           RPILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDN
Sbjct: 242 RPILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDN 301

Query: 308 WISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCD 367
           W SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCD
Sbjct: 302 WNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCD 361

Query: 368 IRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLW 427
           IRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLWNRG +
Sbjct: 362 IRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLWNRGFY 417

Query: 428 RANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           RA ITASWS IGLS STTVTARD+WEHSSQVVQHQLTAQVD HDCKMYVLTPH
Sbjct: 422 RAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYVLTPH 417

BLAST of Lsi04G007660 vs. NCBI nr
Match: XP_038892679.1 (alpha-galactosidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 719.9 bits (1857), Expect = 1.4e-203
Identity = 371/480 (77.29%), Postives = 383/480 (79.79%), Query Frame = 0

Query: 1   MAFPPLPLSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTR 60
           MA PP  LSSPPS +  L SVLFWMFLLGS    NG AVAGA RSR LRFAA FDS STR
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGS----NGGAVAGAIRSRSLRFAAGFDSVSTR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLA+LGYEYINL D +   
Sbjct: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCW--- 120

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       +  ++ +GNLVAKASTFPSGIKALADYVHRKG
Sbjct: 121 ---------------------------AELNRDSKGNLVAKASTFPSGIKALADYVHRKG 180

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAGIQTCSKKMPGSLGHE+QDA+TFASWG                  RYPKMS
Sbjct: 181 LKLGIYSDAGIQTCSKKMPGSLGHEQQDAETFASWGIDYLKYDNCENTGTSPKERYPKMS 240

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KALQQ+GRPILFSLCEW                         GQEDPATWA+NVGNSWRT
Sbjct: 241 KALQQSGRPILFSLCEW-------------------------GQEDPATWAINVGNSWRT 300

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA
Sbjct: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCDIRSMDNI LKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV
Sbjct: 361 PLLIGCDIRSMDNIALKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           LWNRGLWRANITASWSDIGLSTSTTVTARD+WEHSSQVVQH LTAQVDSHDCKMYVLTPH
Sbjct: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMYVLTPH 421

BLAST of Lsi04G007660 vs. NCBI nr
Match: XP_038892678.1 (alpha-galactosidase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 715.3 bits (1845), Expect = 3.4e-202
Identity = 371/481 (77.13%), Postives = 383/481 (79.63%), Query Frame = 0

Query: 1   MAFPPLPLSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTR 60
           MA PP  LSSPPS +  L SVLFWMFLLGS    NG AVAGA RSR LRFAA FDS STR
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGS----NGGAVAGAIRSRSLRFAAGFDSVSTR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLA+LGYEYINL D +   
Sbjct: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCW--- 120

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       +  ++ +GNLVAKASTFPSGIKALADYVHRKG
Sbjct: 121 ---------------------------AELNRDSKGNLVAKASTFPSGIKALADYVHRKG 180

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAGIQTCSKKMPGSLGHE+QDA+TFASWG                  RYPKMS
Sbjct: 181 LKLGIYSDAGIQTCSKKMPGSLGHEQQDAETFASWGIDYLKYDNCENTGTSPKERYPKMS 240

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KALQQ+GRPILFSLCEW                         GQEDPATWA+NVGNSWRT
Sbjct: 241 KALQQSGRPILFSLCEW-------------------------GQEDPATWAINVGNSWRT 300

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA
Sbjct: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSGKRVAV 420
           PLLIGCDIRSMDNI LKLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSGKRVAV
Sbjct: 361 PLLIGCDIRSMDNIALKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAV 420

Query: 421 VLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTP 464
           VLWNRGLWRANITASWSDIGLSTSTTVTARD+WEHSSQVVQH LTAQVDSHDCKMYVLTP
Sbjct: 421 VLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMYVLTP 422

BLAST of Lsi04G007660 vs. NCBI nr
Match: XP_008445911.1 (PREDICTED: alpha-galactosidase [Cucumis melo])

HSP 1 Score: 704.5 bits (1817), Expect = 6.0e-199
Identity = 361/471 (76.65%), Postives = 378/471 (80.25%), Query Frame = 0

Query: 10  SPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGLA 69
           S PSVMV LY VL W FLLG  G+GNG AVAGA+RS  LRFAAEFDS S+RRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIVG 129
           LTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINL D +            
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCW------------ 125

Query: 130 LNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 189
                              +  ++ +GNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA
Sbjct: 126 ------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 185

Query: 190 GIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGRP 249
           GIQTCSKKMPGSLG+EEQDAKTFASWG                  RYPKM+KALQQ+GRP
Sbjct: 186 GIQTCSKKMPGSLGNEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRP 245

Query: 250 ILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNWI 309
           ILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDNWI
Sbjct: 246 ILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDNWI 305

Query: 310 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDIR 369
           SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCDIR
Sbjct: 306 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIR 365

Query: 370 SMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA 429
           SMDNIT+KLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA
Sbjct: 366 SMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRA 419

Query: 430 NITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           NITASWSDIGL +STTVTARD+W+HSSQVVQH LTAQVDSHDCKM+VLTPH
Sbjct: 426 NITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 419

BLAST of Lsi04G007660 vs. NCBI nr
Match: KAA0034096.1 (alpha-galactosidase [Cucumis melo var. makuwa] >TYK15823.1 alpha-galactosidase [Cucumis melo var. makuwa])

HSP 1 Score: 699.9 bits (1805), Expect = 1.5e-197
Identity = 361/472 (76.48%), Postives = 378/472 (80.08%), Query Frame = 0

Query: 10  SPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTRRVLLNNGLA 69
           S PSVMV LY VL W FLLG  G+GNG AVAGA+RS  LRFAAEFDS S+RRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSSLYISLMIVG 129
           LTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINL D +            
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCW------------ 125

Query: 130 LNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 189
                              +  ++ +GNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA
Sbjct: 126 ------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDA 185

Query: 190 GIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMSKALQQAGRP 249
           GIQTCSKKMPGSLG+EEQDAKTFASWG                  RYPKM+KALQQ+GRP
Sbjct: 186 GIQTCSKKMPGSLGNEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRP 245

Query: 250 ILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRTTSDIQDNWI 309
           ILFSLCEW                         GQEDPATWAVNVGNSWRTTSDIQDNWI
Sbjct: 246 ILFSLCEW-------------------------GQEDPATWAVNVGNSWRTTSDIQDNWI 305

Query: 310 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKAPLLIGCDIR 369
           SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIWALAKAPLLIGCDIR
Sbjct: 306 SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIR 365

Query: 370 SMDNITLKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR 429
           SMDNIT+KLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR
Sbjct: 366 SMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWR 420

Query: 430 ANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           ANITASWSDIGL +STTVTARD+W+HSSQVVQH LTAQVDSHDCKM+VLTPH
Sbjct: 426 ANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 420

BLAST of Lsi04G007660 vs. NCBI nr
Match: NP_001267530.1 (alpha-galactosidase-like precursor [Cucumis sativus] >ABC88435.1 acid alpha galactosidase 2 [Cucumis sativus] >KGN51578.1 hypothetical protein Csa_008622 [Cucumis sativus])

HSP 1 Score: 694.1 bits (1790), Expect = 8.1e-196
Identity = 354/480 (73.75%), Postives = 378/480 (78.75%), Query Frame = 0

Query: 1   MAFPPLPLSSPPSVMVPLYSVLFWMFLLGSNGSGNGSAVAGATRSRPLRFAAEFDSASTR 60
           MA PP    S PSVM+ LY +LFW FLLG NG+GNG  VA A+RS  LRFA EFDSAS+R
Sbjct: 1   MALPP----SSPSVMLLLYFLLFWTFLLGGNGNGNGVVVAAASRSTALRFATEFDSASSR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           R+LLNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMVS+GLAALGY+YINL D +   
Sbjct: 61  RILLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCW--- 120

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       +  ++ +GNLVAKASTFPSGIKALADYVHRKG
Sbjct: 121 ---------------------------AELDRDSKGNLVAKASTFPSGIKALADYVHRKG 180

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAGI+TCSK+MPGSLGHEEQDAKTFASWG                  RYPKM+
Sbjct: 181 LKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMT 240

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KALQQ+GRPILFSLCEW                         GQEDPATWAVNVGNSWRT
Sbjct: 241 KALQQSGRPILFSLCEW-------------------------GQEDPATWAVNVGNSWRT 300

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMT  EYRSHFSIWALAKA
Sbjct: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWALAKA 360

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCDIRSMDN T+KLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAG LSGKRVAVV
Sbjct: 361 PLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRVAVV 420

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSSQVVQHQLTAQVDSHDCKMYVLTPH 464
           LWNR LWRANITA WSDIGLS+STTVTARD+WEHSSQVV+H LTAQVDSHDCKM+VLTPH
Sbjct: 421 LWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVLTPH 421

BLAST of Lsi04G007660 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 527.7 bits (1358), Expect = 9.3e-150
Identity = 268/419 (63.96%), Postives = 303/419 (72.32%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+ D +   
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG-- 88

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       + +++ QG+LVAKASTFPSGIKAL+DYVH KG
Sbjct: 89  ----------------------------ELKRDSQGSLVAKASTFPSGIKALSDYVHSKG 148

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAG  TCS+ MPGSLGHEEQDAKTFASWG                  RYPKMS
Sbjct: 149 LKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMS 208

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KAL  +GR I FSLCEW                         GQEDPATWA ++GNSWRT
Sbjct: 209 KALLNSGRSIFFSLCEW-------------------------GQEDPATWAGDIGNSWRT 268

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           T DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIWALAKA
Sbjct: 269 TGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALAKA 328

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS KRVAV+
Sbjct: 329 PLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVI 388

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSS-QVVQHQLTAQVDSHDCKMYVLT 462
           LWNRG   ANITA W++IGL++S  V ARD+WEHS+   V+ QL+A V+ H CKMY LT
Sbjct: 389 LWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKMYTLT 392

BLAST of Lsi04G007660 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 527.7 bits (1358), Expect = 9.3e-150
Identity = 268/419 (63.96%), Postives = 303/419 (72.32%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLGDDYNSS 120
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+ D +   
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG-- 62

Query: 121 LYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALADYVHRKG 180
                                       + +++ QG+LVAKASTFPSGIKAL+DYVH KG
Sbjct: 63  ----------------------------ELKRDSQGSLVAKASTFPSGIKALSDYVHSKG 122

Query: 181 LKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY-----------------RYPKMS 240
           LKLGIYSDAG  TCS+ MPGSLGHEEQDAKTFASWG                  RYPKMS
Sbjct: 123 LKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMS 182

Query: 241 KALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNVGNSWRT 300
           KAL  +GR I FSLCEW                         GQEDPATWA ++GNSWRT
Sbjct: 183 KALLNSGRSIFFSLCEW-------------------------GQEDPATWAGDIGNSWRT 242

Query: 301 TSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWALAKA 360
           T DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIWALAKA
Sbjct: 243 TGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALAKA 302

Query: 361 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 420
           PLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS KRVAV+
Sbjct: 303 PLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVI 362

Query: 421 LWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSS-QVVQHQLTAQVDSHDCKMYVLT 462
           LWNRG   ANITA W++IGL++S  V ARD+WEHS+   V+ QL+A V+ H CKMY LT
Sbjct: 363 LWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKMYTLT 366

BLAST of Lsi04G007660 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-125
Identity = 233/427 (54.57%), Postives = 279/427 (65.34%), Query Frame = 0

Query: 55  DSASTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLG 114
           DS   RR LL NGL +TPPMGWNSWNHF CNI+E +IKETADA+V++GL+ LGY Y+N+ 
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DDYNSSLYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGIKALAD 174
           D +                              E SR + +G+LV K STFPSGIKA+AD
Sbjct: 97  DCW-----------------------------AEISRDS-KGSLVPKKSTFPSGIKAVAD 156

Query: 175 YVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWG-----------------Y 234
           YVH KGLKLGIYSDAG  TCSK MPGSLG+EE DAKTFA WG                  
Sbjct: 157 YVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTV 216

Query: 235 RYPKMSKALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPATWAVNV 294
           RYP M++AL ++GRPI  SLCEW                         G   PA W   V
Sbjct: 217 RYPVMTRALMKSGRPIFHSLCEW-------------------------GDMHPALWGSPV 276

Query: 295 GNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSI 354
           GNSWRTT+DI+D W+SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY  HFSI
Sbjct: 277 GNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSI 336

Query: 355 WALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSG 414
           WA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWAGPLSG
Sbjct: 337 WAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSG 396

Query: 415 KRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHSS--QVVQHQLTAQVDSHDC 463
            RVA++L NRG  R +ITA W DI +  ++ V ARD+WEH +  Q     LTA VDSH C
Sbjct: 397 YRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHAC 408

BLAST of Lsi04G007660 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 432.2 bits (1110), Expect = 5.3e-121
Identity = 229/435 (52.64%), Postives = 272/435 (62.53%), Query Frame = 0

Query: 50  FAAEFDSASTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYE 109
           F + +D++   R+ LNNGLA TP MGWNSWN F CNINE +IKETADA+VSSGLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 110 YINLGDDYNSSLYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGI 169
           ++N+ D +++ L                              ++ +G LV    TFPSGI
Sbjct: 111 HVNIDDCWSNLL------------------------------RDSEGQLVPHPETFPSGI 170

Query: 170 KALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY------------ 229
           K LADYVH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG             
Sbjct: 171 KLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLG 230

Query: 230 -----RYPKMSKALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPAT 289
                RYP M  AL   GR I +SLCEW                         G +DPA 
Sbjct: 231 IKPIERYPPMRDALNATGRSIFYSLCEW-------------------------GVDDPAL 290

Query: 290 WAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYR 349
           WA  VGNSWRTT DI D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR
Sbjct: 291 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYR 350

Query: 350 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGD---LE 409
            HFSIWAL KAPLLIGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+    +
Sbjct: 351 GHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQ 410

Query: 410 VWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHS--SQVVQHQLT 463
           VW+GPLSG R+ V LWNR    A ITASW  IGL ++ +V+ RD+W+H   ++       
Sbjct: 411 VWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFE 429

BLAST of Lsi04G007660 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 406.0 bits (1042), Expect = 4.1e-113
Identity = 220/435 (50.57%), Postives = 257/435 (59.08%), Query Frame = 0

Query: 50  FAAEFDSASTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYE 109
           F + +D++   R+ LNNGLA TP MGWNSWN F CNINE +IKETADA+VSSGLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 110 YINLGDDYNSSLYISLMIVGLNLTETLRMSMKISIFALEDSRQNFQGNLVAKASTFPSGI 169
           ++N+                                                      GI
Sbjct: 111 HVNI------------------------------------------------------GI 170

Query: 170 KALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGY------------ 229
           K LADYVH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG             
Sbjct: 171 KLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLG 230

Query: 230 -----RYPKMSKALQQAGRPILFSLCEWLAIFIHKKKHASVVLMLLHSKAQTEGQEDPAT 289
                RYP M  AL   GR I +SLCEW                         G +DPA 
Sbjct: 231 IKPIERYPPMRDALNATGRSIFYSLCEW-------------------------GVDDPAL 290

Query: 290 WAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYR 349
           WA  VGNSWRTT DI D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR
Sbjct: 291 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYR 350

Query: 350 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKYGD---LE 409
            HFSIWAL KAPLLIGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+    +
Sbjct: 351 GHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQ 405

Query: 410 VWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDVWEHS--SQVVQHQLT 463
           VW+GPLSG R+ V LWNR    A ITASW  IGL ++ +V+ RD+W+H   ++       
Sbjct: 411 VWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFE 405