Lsi04G007600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G007600
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING-type domain-containing protein
Locationchr04: 7090662 .. 7096985 (+)
RNA-Seq ExpressionLsi04G007600
SyntenyLsi04G007600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTTTCTAACAGTCGGGTCAATCTTAAACTTAATCATTTATACATTTCGAGAGCTTTTGTGGATATTTTGAGGAGACTGGTGGTCGGGAACCCGACGCTAATCGGAAAGCGACATTGTATGCAGAAATCATCGTAGTTTTGGGGATTTTCAATTCGTGCGAACTTCTTAGGGCATTGATTTTCAGGTTTCATCTTCGTCTTTGTGTTGTGATTCGGTTGAGTTCTTTCAAATTTCGATTACATAGAAAAAGTAAGCTCTGAATTTGAGTTGTTTTAGAGGGAGATCGGTCCAGAATCGAGATGACTCCTGTTTGTCCTTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGGGGGAATCTGGTTGTCCTTTCGCGAAATCCGGCCGTTCCGATGATGCTTCTTCAAGAAAGCCGCAAACGGAGGCTGCTTGTCCGTTTTCGAAATCCGTTCGTTCGGATGATGCTTCATCGAGGAAGAATCAGGCTGAGGCGGAGAGTAATGGAGCGGAGAAAGATATTGCCGATGCTGCTGGAACCGGTGGCAAGTGTCCATTCGGATATGATTCTCAAACTTTCAAGATTGGCCCTCTGAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAGTAATAATTAATCAGCTATGGTGTTTCTTATCTTCGTTTTGTTTTTCTTTTCGAGATCTCTTAGGTCATTTATTTCTTTGTTTCTTCTTGAATTGAACTAGAGCATGCATATCGCGTTTTAATGACTGTCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCAGACGCCAATCTTCAAGCTACGGTCGATCGCTTCATCGAAGGTCATGCGAGAATCAAGAGACCCCAGGTGAGCTCAGACAAAGAGCAGGAGAAAGTTAGTGAAAGTAAAGTAGTGATTTATGAAGACGTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGTAATATTCCTTGTTCATTTCTTCCTAATACGTTTTTGCTTGTGAATTGGATCTTGGTTTTGAAATCCTTTTATTTGAACGATAGTCAGTGGTGGTGTGAGCTTCATTTTCTCTGGGGCTGTCTGATTTCTTTTTTTAAACGATTTTCTATTATTGTTTTCCAAACTATTTTTGCCAATGCTGATTGAAGAACTCATAGAAATTATTGCTATTATGCCGGTAAAAATTACTTTTCATACATTCATAAAAATGAATAAGTAGTTTCCCCTTTTTTGCAAGGATCATTTCTAACTAGAAGGCTCTTCTTTTACAGGTTTCATCTCCCTGGCCCCTTTGTAGCATTGTCCTTTTTTTTTTTTTTATACCTTTTCTCTGTTTATTTTTTGGGAAAAGGATAGAAAATTTTTCATTGACAAAATGAAAAGAGGTTGATGCTCAAAGGATACAAACTCCACATGAGAGTGAATAAGGAAAGTGACAAAATAAATCCTCCATAAATAATGGAACAACTTTTCGACGAAAAGACAAAAGATATTCTAAATAAGACATATCCTGTAAAGAGAAGCCAACAAAATATTTGAGAATAGAGCACCAGGATGAAGCTTTGATACCAGCTTTATCAAACCAAACCTTTCATAACATAAGTCAATACACATCAAGAAAAACAAACTTAAAGTTTGGAATCCAAGCCCTAAGGATTGAAAAGTTGTGTGTCCAACATAAACCATCAATAAAAGAAGAATTACAAATGAATTTCAACGACTGTGGCCTTCGAAGAAGCTAAAATCCAAAACATCCACCATGACCTATCCATACTAAGTAGAATCCTTCACAAACTTCAAAGCAAAAGCTAAACCAAAAGGATAACTTTAATCAAGATTGAAATCAAAAGGGACAAAATCAAGCTAGGAAGTAGGAACATGTAAATCTCTTCAAACATCAATCTTCAACACCACAAACTCCAGACATGGTAATGCAGACAGAAGAGTAGCCAAACCAAATATCCAAAAAAACCCAAACAAAATAGAACCTTAGGTCTTTGAGGCACCACAATCTTCTTGTATGCCAGGACACTTTCTACATTGGAATATAAAAAAAAAGCAAAGAATAATTAAGCTTTTATTCTACCTGTCAGGAACAAAAGAGGGAATCTCTTTAAATTGCTGACCACACACTTTGGTAAGTTATCTAAAGCAAAATCCTCAATCCTATCTTCTTCGTAGAAGCTACTATGATGTTGAAAGTCAATTTCCAACCTTATCTTCTAAAACATCCGAGACGACAATATAGGGCAAGATTGATCATTGTGTGGTGGCCTGGTGGGTTTGTTTTAGAAGATATTAAAACCTTATCAAAATGTGTATGATTGAGCCTAACATTACAACGTTCTCCACTTAATTTCTATGATGAATCCTTGGGAAGACCTTCTTTCTTTGAGCCCAAAATGTGTACCTCTCCGTTTCTTATAAATAATAATAATAAGAAGAAATGGAATAAGTAGTTTCATCTGTCAGAACACACAAAAAGAAAGCAAGAAAAAACAACTTTTTGGAAACAGTTATCCAAACACCGTTTGCAGAAAATAAACATCCCATTAGTTTCTGTTTTTAAAAGTTTTACAATCTGGCTTTTTATTGCAGGCATTTCGTGCCCAAAATATTGGAAGTGCCAAATCCAGGCTGACTGTCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAATGGGCAGTTCATCCGAATTGTGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGGTAAGTTTGCTCCCTTCTCTTTCTTATGATGGGACATATAATTTCTAATGCAGCTTCCCTTCGTTATCGTCTTATTAAGAGTCTTACATATTGTTTTGTAAGCAATTTAATTTAATTTATTAGTTATTGATGAGTGTTTGTTGGTGTTTTTGGTGTATTGATGGTTGTGGAAAAAATAAGCAGGCCTAACCCAGGATTTTGACGTCCGATTGTATTGGGCTCATGGCCAACTAATAGGTTTATCGGGCAAGCAGTTTTTGTAGTTATCCCTTTTATCTTTAGAGAAGAAACATTTCATTGATACAGATTTTGTGGTTATCATGCTAACAAACAACTAACACCGTAATTAAATGAAATCCTGCTGTCAATATTCCATCGCAATTAACCAAATGGATATTATGTAGCTGCTTAACTACAATCCTGATGTAAATTTCTGTTGTTTGATCCAATTTTACAGTCGAGCAGCTGGAGATGCTAGTTCAGCAATCAAGCACTTTGAAGAAAGTGTAGAATTTCTCTCAAAACTGCCTGAAAAGACTCACGAGGTTCTTTATTTGAAACATTTGTGAGTTAACTTTGTGATTTATTAACTGCTTGCGTTTGCAATACTCAGTAACCCTTGTTTCTCCCTTCAGATCACTCATACACTTTCAGTATCACTAAATAAAATTGGCGATCTAAAGTATTATGAAGGAGACCTCCAAGCTGCAAGATCTTATTATATCCGGTCTCTTAATGTTCGCCAAGATGCGAGCAAGCATCATCCAGATGATCCAGCTCAGGTTTGTGATTGTTAATTTCTTATATGCAATCCTTTATCCTAAAATATATAACATTTTCTCTATGTTTTCCAATTCTAGAAGCTTTAAGTGATAAGTGGTTTTATTTAGTGTTTATCAAACTCGTATGATACACTTGTATTGTACAAGTTGACTCAAAACGAGGTCAAATTGATTGTGAATCGTACGAATCAGACTGAATCGCAATGATCTGATTCAAATTCAATATCCTTGCAACAAAGCATAAGGGAAAAAACCTTAACTAAAAAATATCGGCAATTAGCACGAACAAAATAACTAGAGAATAAGGCAAATAAAACAATGCCAGTGATAGTGTGCATGAATAAAAAAATCCGAGAAGTTAGTTGGACTTGTGGTGATTATAGAGGATGAAACTCAAAGAGGAAGAAAATGAAAGACTTTGGGTTAACAACCTTGGTTCCCATAAGGTAATTCGTCAACCATTACGGATGGATGTTAATATAGGATCAGCGTTCCGAGTTGTAATGAACAGAAACTAATTTGTGAAGTCATCAACTGCATCTTGAACGCTGTGAAATAAAAAACTTAAGGAATCCTTCCAAATGGACTGGCAAGAGAGCACAAGAATACAAAAGACTGACTCGTCTCCTTTCTTTTGCATTCCACGTGCTTATAAAGGGATGGATATATGTACCTTACTGATGTACATATACAAATCAATATGATAGAGTACCCTATCTGAATATAGCTCGATTAGTTAAAGCATTTGTTCTCAACCAAAAGATTAGAAGTTTGAGTCTCCCAACTCTCACTTCTTGAACTCAAATCAAATTGTAGTAAATTAGAAGGAATAGTTTGAATGTTTATGAAAAATTTGAACTGTTGGCTTTTAGTTAGTCAATTTGTTCCCTATAATTTGATGTAGGAGATTTGTAGGGATAGTTTGAAGATAAGTTTACCGGATTAGTGTTTTAGATATTGGCTTTTAGTTAGTAAATTTCTCTATAAATTTTATAGAGGCATTAAACTCTTCAATTAGGGACCTCGTAACAAGTTTGATATTGAATATAGATTTTCTTTGAGGATTCTTAGAGGGTGAATGTTAGCCTGCATCATTTGCATCAGTACCATGTTTCCCCCAAGCATAAATAACTGTTGTAAATCGAGACCAGAAACTTCTATAGAAACAAATTCCAAGAATGAGTAGAAGAACTTAATAGATTTTCTCCTCAGATTAGAATTTGCCAGAAGAATGGCTATTTTGGTGAACCCATTGTAGTCTACATGATTAGTTGGTTGAGTATTCTGCAAATGCTTATTGTTGACCCTTTGGGACTTAAGTATATGATTGGCAATTTCCCAAGAGTCCAATAACTGGTGAGTGTAGGATATCTTGCCTAGGTGATACGCATGGTGTTTACAAAGACTTTGATTGTGGATTTCATCTTTGCATTAAGATACCTTCAAAAAAAATGTTAGTTACTTATTTTAATCCAAATTACAAATTTGGTTCTTAGACTTTGATTATTATTATTATTATTGTTATTCTTTTTTTTTTTAAGTTCACAATCTACTATATACGAAATTGAAGAATGCGATAGGATATCTAGCTTGGGTGATACACATGGTGTTTTCAAAGACTTTAATTGTAGATTTCATTGTTGCATTAACATACCTTTTTAAAGGTTAAATTGCTTATTTAGTCCTTGAACTTTCAAAATTATAAATCAAGCTTGGACTTGTTTTTGTCCTTTAACTTTACAAATCTACTCTACACAAAAATTGAAAGTTTATGGTTCTATTTGACATAAAACTCACATTTATATTTAATAGACAAGTAATTTTAAAAATATTGAATTTTTTAGAGACATATTAAACACAAAATTGAAAACTGATGAACTTAATAGATACAAAATTGAAAGTTGGGATCTATTAGATGCTTCTAAGAGTTCAAAAACCAAACAGTCACAAATTTGATAGTTTCCTCAATACATCAATACTTCTAAAATGGCTATTTCTTATTGCTTTCAACAGATTCTAGATGTGGCTGTTTCTCTTGCAAAAGTGGCAGATGTAGACAGTAGTCTAGGAAATGAGGATATGGCAGTTGATAGATTTCAGGAAGGCATTAAGTTGTTGGAATCATTAACACTGAATTCTGAGAATCCTGGTCTTCAACAACGAGTAAGACTAAACGTCTTTGCTTGCTTTCTTTCACTTAATACTTCATATCAATAAAAAATTTGATGGGATTTTTATATTTTTCTGGCAGCGTGAATCTGTTTTGAAGTTCCTTGAAGGTCAACTTGCAGAGCGACAAAAGCCATCTCAATCCAGTTGATAGAGATCATTCCAATCTTCTATATGCAAATAAGCTCTCCCTTTAGCCAAGCAACAGGGAAGATTGCGAGTGTTAAAGGTATAAATTTATCGCCACGGTTCAGGGTATAGTACAGAAGCATACGTAATCGATGTTGCTTTATTCATATGAGCATGAATCGGCCTGGCAGTCACGTCTCTTGTGTATAATGTTTTTCTTAGTTCTCTTTGAGAAACTCTGGTTTTGTACAATTTAGCAGGAACTCCTCACGCTCTTTTATAGTGAAGTTGTTTGCTAAGATGCAATCTATGAAATAAATTGGAACTTAAATAGTTTATAGTATTTGGTCATTCCCATTCCCCTTTTTTGGTGATTTGCGTTTGATATTTTACTAGTTATATAAATGCTGAAAATGGTTATGAGATGGTTTCTTTTCTTTTA

mRNA sequence

CTTTCTTTCTAACAGTCGGGTCAATCTTAAACTTAATCATTTATACATTTCGAGAGCTTTTGTGGATATTTTGAGGAGACTGGTGGTCGGGAACCCGACGCTAATCGGAAAGCGACATTGTATGCAGAAATCATCGTAGTTTTGGGGATTTTCAATTCGTGCGAACTTCTTAGGGCATTGATTTTCAGAGGGAGATCGGTCCAGAATCGAGATGACTCCTGTTTGTCCTTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGGGGGAATCTGGTTGTCCTTTCGCGAAATCCGGCCGTTCCGATGATGCTTCTTCAAGAAAGCCGCAAACGGAGGCTGCTTGTCCGTTTTCGAAATCCGTTCGTTCGGATGATGCTTCATCGAGGAAGAATCAGGCTGAGGCGGAGAGTAATGGAGCGGAGAAAGATATTGCCGATGCTGCTGGAACCGGTGGCAAGTGTCCATTCGGATATGATTCTCAAACTTTCAAGATTGGCCCTCTGAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAAGCATGCATATCGCGTTTTAATGACTGTCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCAGACGCCAATCTTCAAGCTACGGTCGATCGCTTCATCGAAGGTCATGCGAGAATCAAGAGACCCCAGGTGAGCTCAGACAAAGAGCAGGAGAAAGTTAGTGAAAGTAAAGTAGTGATTTATGAAGACGTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGCATTTCGTGCCCAAAATATTGGAAGTGCCAAATCCAGGCTGACTGTCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAATGGGCAGTTCATCCGAATTGTGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGTCGAGCAGCTGGAGATGCTAGTTCAGCAATCAAGCACTTTGAAGAAAGTGTAGAATTTCTCTCAAAACTGCCTGAAAAGACTCACGAGATCACTCATACACTTTCAGTATCACTAAATAAAATTGGCGATCTAAAGTATTATGAAGGAGACCTCCAAGCTGCAAGATCTTATTATATCCGGTCTCTTAATGTTCGCCAAGATGCGAGCAAGCATCATCCAGATGATCCAGCTCAGTGTTTATCAAACTCGTATGATACACTTGTATTGTACAAGTTGACTCAAAACGAGGTCAAATTGATTGTGAATCATGTGGCTGTTTCTCTTGCAAAAGTGGCAGATGTAGACAGTAGTCTAGGAAATGAGGATATGGCAGTTGATAGATTTCAGGAAGGCATTAAGTTGTTGGAATCATTAACACTGAATTCTGAGAATCCTGGTCTTCAACAACGACGTGAATCTGTTTTGAAGTTCCTTGAAGGTCAACTTGCAGAGCGACAAAAGCCATCTCAATCCAGTTGATAGAGATCATTCCAATCTTCTATATGCAAATAAGCTCTCCCTTTAGCCAAGCAACAGGGAAGATTGCGAGTGTTAAAGGTATAAATTTATCGCCACGGTTCAGGGTATAGTACAGAAGCATACGTAATCGATGTTGCTTTATTCATATGAGCATGAATCGGCCTGGCAGTCACGTCTCTTGTGTATAATGTTTTTCTTAGTTCTCTTTGAGAAACTCTGGTTTTGTACAATTTAGCAGGAACTCCTCACGCTCTTTTATAGTGAAGTTGTTTGCTAAGATGCAATCTATGAAATAAATTGGAACTTAAATAGTTTATAGTATTTGGTCATTCCCATTCCCCTTTTTTGGTGATTTGCGTTTGATATTTTACTAGTTATATAAATGCTGAAAATGGTTATGAGATGGTTTCTTTTCTTTTA

Coding sequence (CDS)

ATGACTCCTGTTTGTCCTTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGGGGGAATCTGGTTGTCCTTTCGCGAAATCCGGCCGTTCCGATGATGCTTCTTCAAGAAAGCCGCAAACGGAGGCTGCTTGTCCGTTTTCGAAATCCGTTCGTTCGGATGATGCTTCATCGAGGAAGAATCAGGCTGAGGCGGAGAGTAATGGAGCGGAGAAAGATATTGCCGATGCTGCTGGAACCGGTGGCAAGTGTCCATTCGGATATGATTCTCAAACTTTCAAGATTGGCCCTCTGAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAAGCATGCATATCGCGTTTTAATGACTGTCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCAGACGCCAATCTTCAAGCTACGGTCGATCGCTTCATCGAAGGTCATGCGAGAATCAAGAGACCCCAGGTGAGCTCAGACAAAGAGCAGGAGAAAGTTAGTGAAAGTAAAGTAGTGATTTATGAAGACGTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGCATTTCGTGCCCAAAATATTGGAAGTGCCAAATCCAGGCTGACTGTCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAATGGGCAGTTCATCCGAATTGTGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGTCGAGCAGCTGGAGATGCTAGTTCAGCAATCAAGCACTTTGAAGAAAGTGTAGAATTTCTCTCAAAACTGCCTGAAAAGACTCACGAGATCACTCATACACTTTCAGTATCACTAAATAAAATTGGCGATCTAAAGTATTATGAAGGAGACCTCCAAGCTGCAAGATCTTATTATATCCGGTCTCTTAATGTTCGCCAAGATGCGAGCAAGCATCATCCAGATGATCCAGCTCAGTGTTTATCAAACTCGTATGATACACTTGTATTGTACAAGTTGACTCAAAACGAGGTCAAATTGATTGTGAATCATGTGGCTGTTTCTCTTGCAAAAGTGGCAGATGTAGACAGTAGTCTAGGAAATGAGGATATGGCAGTTGATAGATTTCAGGAAGGCATTAAGTTGTTGGAATCATTAACACTGAATTCTGAGAATCCTGGTCTTCAACAACGACGTGAATCTGTTTTGAAGTTCCTTGAAGGTCAACTTGCAGAGCGACAAAAGCCATCTCAATCCAGTTGA

Protein sequence

MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDASSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVSESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYYEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVSLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQKPSQSS
Homology
BLAST of Lsi04G007600 vs. ExPASy Swiss-Prot
Match: Q9M2V1 (Protein NCA1 OS=Arabidopsis thaliana OX=3702 GN=NCA1 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 4.8e-137
Identity = 267/419 (63.72%), Postives = 313/419 (74.70%), Query Frame = 0

Query: 2   TPVCPFVKSARPDDASSRKHQGE---SGCPFAKSGRSDDASSRK--PQTEAACPFSKSVR 61
           T VCPF K+ARPDD S+RK QGE   SGCPF+K+ R DDAS+RK    T + CPFSKS R
Sbjct: 4   TSVCPFSKAARPDDGSTRK-QGEITASGCPFSKAARPDDASARKQGETTASGCPFSKSAR 63

Query: 62  SDDASSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSR 121
            D+  S K   E E N   KD  D+A    KCPFGYDSQTFK+GP SCM+CQALL+E SR
Sbjct: 64  PDENGS-KGCPEQEGN-LNKDSTDSATVPAKCPFGYDSQTFKLGPFSCMLCQALLYESSR 123

Query: 122 CVPCSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQ 181
           CVPC+H+FCK C++RF DCPLCGADIE IE D NLQ  VD+FIEGHARIKR  V+  +++
Sbjct: 124 CVPCTHVFCKVCLTRFKDCPLCGADIESIEVDENLQKMVDQFIEGHARIKRSVVNGTEKE 183

Query: 182 EKVSESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSS 241
           E  +++K VIY DVSMERG+FL+QQAMRAF AQN  SAKSRL +C EDIRDQL R G++ 
Sbjct: 184 EVENDNKKVIYADVSMERGSFLVQQAMRAFSAQNYESAKSRLAMCTEDIRDQLGREGNTP 243

Query: 242 ELCSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGD 301
           ELCSQLGAVLG LGDC RA GD+SSA+KHFEESVEFL KLP    EITHTLSVSLNKIGD
Sbjct: 244 ELCSQLGAVLGMLGDCSRAMGDSSSAVKHFEESVEFLMKLPLNDLEITHTLSVSLNKIGD 303

Query: 302 LKYYEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNH 361
           LKYY+ DLQAARSYY R+LNVR+DA KHHP+ P+Q L                       
Sbjct: 304 LKYYDEDLQAARSYYDRALNVRRDAMKHHPNAPSQILD---------------------- 363

Query: 362 VAVSLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQL 416
           VAVSLAKVAD+D +L NE  A D F+EG++LLESL L+SE+  L+QRR SVL+FL+ Q+
Sbjct: 364 VAVSLAKVADIDRTLQNEVAATDGFKEGMRLLESLKLDSEDSALEQRRLSVLEFLKKQV 397

BLAST of Lsi04G007600 vs. ExPASy Swiss-Prot
Match: A5WW08 (E3 ubiquitin-protein ligase CHFR OS=Danio rerio OX=7955 GN=chfr PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.3e-05
Identity = 31/96 (32.29%), Postives = 46/96 (47.92%), Query Frame = 0

Query: 102 LSCMICQALLFECSRCVPCSHIFCKACIS----RFNDCPLCGADIEKIEADANLQATVDR 161
           L+C+ICQ LL++C    PC H FC AC S    R + CP C   +E+I  +  L   V+ 
Sbjct: 275 LTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEA 334

Query: 162 FIEGH-----------ARIKRPQVSSDKEQEKVSES 183
           ++  H           +   R +++ D  Q KV  S
Sbjct: 335 YLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERS 370

BLAST of Lsi04G007600 vs. ExPASy Swiss-Prot
Match: Q810L3 (E3 ubiquitin-protein ligase CHFR OS=Mus musculus OX=10090 GN=Chfr PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 7.4e-05
Identity = 30/93 (32.26%), Postives = 48/93 (51.61%), Query Frame = 0

Query: 102 LSCMICQALLFECSRCVPCSHIFCKACIS----RFNDCPLCGADIEKIEADANLQATVDR 161
           L+C+ICQ LL +C    PC H FC AC S    R + CP C   +E+I  +  L   V+ 
Sbjct: 301 LTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEA 360

Query: 162 FIEGHARIKRPQVSSDKEQEKVSESKVVIYEDV 191
           ++     I+ P  S  +E  +  +++  I +D+
Sbjct: 361 YL-----IQHPDKSRSEEDVRSMDARNKITQDM 388

BLAST of Lsi04G007600 vs. ExPASy Swiss-Prot
Match: Q5FWP4 (E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.8e-04
Identity = 53/240 (22.08%), Postives = 93/240 (38.75%), Query Frame = 0

Query: 6   PFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDASSRKN 65
           P ++++ P    S + QG  G P  +    D  ++ K    A+CP   S  S   S + +
Sbjct: 199 PAIRASIPKSNLSTQEQGSLGPPKKRIRTEDHWTTNKNFVPASCPIGASDESKTPSMKPD 258

Query: 66  QAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIFC 125
           + E                                 L+C+ICQ LL +C    PC H FC
Sbjct: 259 KMEE-------------------------------TLTCIICQELLHDCVSLQPCMHTFC 318

Query: 126 KACIS----RFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVSE 185
            AC S    R + CP C   +E+I  +  L   V+ ++     I+ P+    +E     +
Sbjct: 319 AACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYL-----IQHPEKCRSEEDRCSMD 378

Query: 186 SKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCSQ 242
           ++  I +D        ++Q  +R   +   GS++  L   + D+  +   +     +C Q
Sbjct: 379 ARNKITQD--------MLQPKVRRSFSDEEGSSEDLLE--LSDVDSESSDISQPYTVCRQ 392

BLAST of Lsi04G007600 vs. ExPASy TrEMBL
Match: A0A0A0KPN8 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G581690 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.2e-205
Identity = 376/426 (88.26%), Postives = 391/426 (91.78%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+E+ACP SKSVRSDDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSESACPVSKSVRSDDA 60

Query: 61  SS-RKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
           SS +KNQAEAESNGAEKD+ADAAG GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP
Sbjct: 61  SSLKKNQAEAESNGAEKDVADAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKV 180
           C+HIFCKACISRFNDCPLCGADIEKIEAD NLQATVDRFIEGHARIKRPQV+SDKEQEKV
Sbjct: 121 CTHIFCKACISRFNDCPLCGADIEKIEADDNLQATVDRFIEGHARIKRPQVNSDKEQEKV 180

Query: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELC 240
           +ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLE+MGSS ELC
Sbjct: 181 NESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAV 360
           YEGDL+AARSYY+RSLNVRQDASKHHPDDPAQ L                       VAV
Sbjct: 301 YEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILD----------------------VAV 360

Query: 361 SLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQ 420
           SLAKVADVDS LGNEDMAVDRFQEGIKLLESL+LNSENP LQ RRESV+KFLEGQLAERQ
Sbjct: 361 SLAKVADVDSGLGNEDMAVDRFQEGIKLLESLSLNSENPALQHRRESVMKFLEGQLAERQ 404

Query: 421 KPSQSS 426
           K +Q+S
Sbjct: 421 KATQTS 404

BLAST of Lsi04G007600 vs. ExPASy TrEMBL
Match: A0A5D3CV76 (Binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003220 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.1e-204
Identity = 375/426 (88.03%), Postives = 388/426 (91.08%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+E+ACPFSKSVRSDDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSESACPFSKSVRSDDA 60

Query: 61  SS-RKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
           SS  KNQAE ESNGAEKD+ DAAG GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP
Sbjct: 61  SSLEKNQAEGESNGAEKDVGDAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKV 180
           CSHIFCKACISRFNDCPLCGADIEK+EAD NLQATVDRFIEGHARIKRPQV+SDKEQEKV
Sbjct: 121 CSHIFCKACISRFNDCPLCGADIEKVEADDNLQATVDRFIEGHARIKRPQVNSDKEQEKV 180

Query: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELC 240
           +ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLE+MGSS ELC
Sbjct: 181 NESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAV 360
           YEGDL+AARSYY+RSLNVRQDASKHHPDDPAQ L                       VAV
Sbjct: 301 YEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILD----------------------VAV 360

Query: 361 SLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQ 420
           SLAKVADVDS LGNEDMAVDRFQEGIKLLESL+LNSENP LQ R ESVLKFLEGQLAERQ
Sbjct: 361 SLAKVADVDSGLGNEDMAVDRFQEGIKLLESLSLNSENPALQHRGESVLKFLEGQLAERQ 404

Query: 421 KPSQSS 426
           K +Q+S
Sbjct: 421 KATQTS 404

BLAST of Lsi04G007600 vs. ExPASy TrEMBL
Match: A0A1S3BEN7 (uncharacterized protein LOC103488802 OS=Cucumis melo OX=3656 GN=LOC103488802 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.1e-204
Identity = 375/426 (88.03%), Postives = 388/426 (91.08%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+E+ACPFSKSVRSDDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSESACPFSKSVRSDDA 60

Query: 61  SS-RKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
           SS  KNQAE ESNGAEKD+ DAAG GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP
Sbjct: 61  SSLEKNQAEGESNGAEKDVGDAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKV 180
           CSHIFCKACISRFNDCPLCGADIEK+EAD NLQATVDRFIEGHARIKRPQV+SDKEQEKV
Sbjct: 121 CSHIFCKACISRFNDCPLCGADIEKVEADDNLQATVDRFIEGHARIKRPQVNSDKEQEKV 180

Query: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELC 240
           +ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLE+MGSS ELC
Sbjct: 181 NESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAV 360
           YEGDL+AARSYY+RSLNVRQDASKHHPDDPAQ L                       VAV
Sbjct: 301 YEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILD----------------------VAV 360

Query: 361 SLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQ 420
           SLAKVADVDS LGNEDMAVDRFQEGIKLLESL+LNSENP LQ R ESVLKFLEGQLAERQ
Sbjct: 361 SLAKVADVDSGLGNEDMAVDRFQEGIKLLESLSLNSENPALQHRGESVLKFLEGQLAERQ 404

Query: 421 KPSQSS 426
           K +Q+S
Sbjct: 421 KATQTS 404

BLAST of Lsi04G007600 vs. ExPASy TrEMBL
Match: A0A6J1CBV4 (protein NCA1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010138 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.2e-197
Identity = 361/420 (85.95%), Postives = 377/420 (89.76%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQ E+ CPFAKSGRSDDASSRK Q+EAACPFSKS R DDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPEAACPFAKSGRSDDASSRKQQSEAACPFSKSARCDDA 60

Query: 61  SSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120
           S RKNQ EAESN AEKD+ADAA TGGKCPFGYDSQTFK+GPLSCMICQALLFECSRCVPC
Sbjct: 61  SVRKNQVEAESNEAEKDVADAATTGGKCPFGYDSQTFKLGPLSCMICQALLFECSRCVPC 120

Query: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVS 180
           SHI+CKACISRF DCPLCGADIEKIEA A+LQ TVDRFIEGHARIKR QV+SDKEQE+VS
Sbjct: 121 SHIYCKACISRFKDCPLCGADIEKIEAHADLQGTVDRFIEGHARIKRSQVNSDKEQEEVS 180

Query: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCS 240
           ESKVVIYEDVSMERGAFLIQQAMRAFRAQN  SAKSRLT+CVEDIRDQLERMG+SSELCS
Sbjct: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNFESAKSRLTICVEDIRDQLERMGNSSELCS 240

Query: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYY 300
           QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEK+HE+THTLSVSLNKIGDLKYY
Sbjct: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKSHEVTHTLSVSLNKIGDLKYY 300

Query: 301 EGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVS 360
           EGDLQAARSYY RSLNVRQDASKHHPDDP+Q L                       VAVS
Sbjct: 301 EGDLQAARSYYFRSLNVRQDASKHHPDDPSQILD----------------------VAVS 360

Query: 361 LAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 420
           LAKVADVDS LGNEDMAV+ FQEGIKLLESLTLNSENPGL+QRR+SVLKFLEGQL ERQK
Sbjct: 361 LAKVADVDSGLGNEDMAVNGFQEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLTERQK 398

BLAST of Lsi04G007600 vs. ExPASy TrEMBL
Match: A0A6J1GY05 (protein NCA1-like OS=Cucurbita moschata OX=3662 GN=LOC111458517 PE=4 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.7e-193
Identity = 362/425 (85.18%), Postives = 373/425 (87.76%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCP VKSARPDDASSRK QGES CPFAKSG SDDASSRK Q+EAACPFSKS RSDDA
Sbjct: 1   MTPVCPSVKSARPDDASSRKQQGESVCPFAKSGCSDDASSRKQQSEAACPFSKSARSDDA 60

Query: 61  SSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120
           S  K+  E E  G     ADAA  GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC
Sbjct: 61  SLTKSPVEGEKGG-----ADAASAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120

Query: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVS 180
           SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQV+SDKE E+VS
Sbjct: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVNSDKENEEVS 180

Query: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCS 240
           ESKVVIYED+SMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMG+SSELCS
Sbjct: 181 ESKVVIYEDLSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGNSSELCS 240

Query: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYY 300
           QLGAVLGTLGDCCRAAGDASSAI HFEESVEFLSKLPEKT EI  TLSVSLNKIGDLKYY
Sbjct: 241 QLGAVLGTLGDCCRAAGDASSAIIHFEESVEFLSKLPEKTREIIQTLSVSLNKIGDLKYY 300

Query: 301 EGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVS 360
           EGDLQAARSYY+RSLNVRQD SKHHPDDPAQ L                       VAVS
Sbjct: 301 EGDLQAARSYYLRSLNVRQDWSKHHPDDPAQILD----------------------VAVS 360

Query: 361 LAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 420
           LAKVADVDS LGNEDMAVDRFQEGIKLLESLTLNSENPGL+QRR+SVLKFLEGQ AERQK
Sbjct: 361 LAKVADVDSGLGNEDMAVDRFQEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQFAERQK 398

Query: 421 PSQSS 426
           P++SS
Sbjct: 421 PNRSS 398

BLAST of Lsi04G007600 vs. NCBI nr
Match: XP_038893051.1 (protein NCA1 [Benincasa hispida] >XP_038893052.1 protein NCA1 [Benincasa hispida])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 387/425 (91.06%), Postives = 394/425 (92.71%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPV PFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+EAACPFSKSVRSDDA
Sbjct: 1   MTPVHPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSEAACPFSKSVRSDDA 60

Query: 61  SSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120
           S RK QAEAESNGAEKD+ADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC
Sbjct: 61  SLRKIQAEAESNGAEKDVADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120

Query: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVS 180
           SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQV+SDKEQEKVS
Sbjct: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVNSDKEQEKVS 180

Query: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCS 240
           ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLERMGSSSELCS
Sbjct: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLERMGSSSELCS 240

Query: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYY 300
           QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLP+KTHEITHTLSVSLNKIGDLKYY
Sbjct: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPDKTHEITHTLSVSLNKIGDLKYY 300

Query: 301 EGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVS 360
           EGDLQAARSYY+RSLNVRQ+ASKHHPDDPAQ L                       VAVS
Sbjct: 301 EGDLQAARSYYLRSLNVRQEASKHHPDDPAQILD----------------------VAVS 360

Query: 361 LAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 420
           LAKVADVDS LGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK
Sbjct: 361 LAKVADVDSGLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 403

Query: 421 PSQSS 426
            +QSS
Sbjct: 421 ATQSS 403

BLAST of Lsi04G007600 vs. NCBI nr
Match: XP_004147090.1 (protein NCA1 [Cucumis sativus] >KGN51585.1 hypothetical protein Csa_009177 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 8.7e-205
Identity = 376/426 (88.26%), Postives = 391/426 (91.78%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+E+ACP SKSVRSDDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSESACPVSKSVRSDDA 60

Query: 61  SS-RKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
           SS +KNQAEAESNGAEKD+ADAAG GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP
Sbjct: 61  SSLKKNQAEAESNGAEKDVADAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKV 180
           C+HIFCKACISRFNDCPLCGADIEKIEAD NLQATVDRFIEGHARIKRPQV+SDKEQEKV
Sbjct: 121 CTHIFCKACISRFNDCPLCGADIEKIEADDNLQATVDRFIEGHARIKRPQVNSDKEQEKV 180

Query: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELC 240
           +ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLE+MGSS ELC
Sbjct: 181 NESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAV 360
           YEGDL+AARSYY+RSLNVRQDASKHHPDDPAQ L                       VAV
Sbjct: 301 YEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILD----------------------VAV 360

Query: 361 SLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQ 420
           SLAKVADVDS LGNEDMAVDRFQEGIKLLESL+LNSENP LQ RRESV+KFLEGQLAERQ
Sbjct: 361 SLAKVADVDSGLGNEDMAVDRFQEGIKLLESLSLNSENPALQHRRESVMKFLEGQLAERQ 404

Query: 421 KPSQSS 426
           K +Q+S
Sbjct: 421 KATQTS 404

BLAST of Lsi04G007600 vs. NCBI nr
Match: XP_008445920.1 (PREDICTED: uncharacterized protein LOC103488802 [Cucumis melo] >KAA0034103.1 binding protein [Cucumis melo var. makuwa] >TYK15817.1 binding protein [Cucumis melo var. makuwa])

HSP 1 Score: 721.5 bits (1861), Expect = 4.3e-204
Identity = 375/426 (88.03%), Postives = 388/426 (91.08%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQGES CPFAKSGRSDDASSRKPQ+E+ACPFSKSVRSDDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQSESACPFSKSVRSDDA 60

Query: 61  SS-RKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
           SS  KNQAE ESNGAEKD+ DAAG GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP
Sbjct: 61  SSLEKNQAEGESNGAEKDVGDAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKV 180
           CSHIFCKACISRFNDCPLCGADIEK+EAD NLQATVDRFIEGHARIKRPQV+SDKEQEKV
Sbjct: 121 CSHIFCKACISRFNDCPLCGADIEKVEADDNLQATVDRFIEGHARIKRPQVNSDKEQEKV 180

Query: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELC 240
           +ESKVVIYEDVSMERGAFLIQQAMRAFRAQNI SAKSRLTVCVEDIRDQLE+MGSS ELC
Sbjct: 181 NESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAV 360
           YEGDL+AARSYY+RSLNVRQDASKHHPDDPAQ L                       VAV
Sbjct: 301 YEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILD----------------------VAV 360

Query: 361 SLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQ 420
           SLAKVADVDS LGNEDMAVDRFQEGIKLLESL+LNSENP LQ R ESVLKFLEGQLAERQ
Sbjct: 361 SLAKVADVDSGLGNEDMAVDRFQEGIKLLESLSLNSENPALQHRGESVLKFLEGQLAERQ 404

Query: 421 KPSQSS 426
           K +Q+S
Sbjct: 421 KATQTS 404

BLAST of Lsi04G007600 vs. NCBI nr
Match: XP_022139165.1 (protein NCA1 isoform X1 [Momordica charantia] >XP_022139166.1 protein NCA1 isoform X1 [Momordica charantia])

HSP 1 Score: 697.2 bits (1798), Expect = 8.8e-197
Identity = 361/420 (85.95%), Postives = 377/420 (89.76%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCPFVKSARPDDASSRKHQ E+ CPFAKSGRSDDASSRK Q+EAACPFSKS R DDA
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPEAACPFAKSGRSDDASSRKQQSEAACPFSKSARCDDA 60

Query: 61  SSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120
           S RKNQ EAESN AEKD+ADAA TGGKCPFGYDSQTFK+GPLSCMICQALLFECSRCVPC
Sbjct: 61  SVRKNQVEAESNEAEKDVADAATTGGKCPFGYDSQTFKLGPLSCMICQALLFECSRCVPC 120

Query: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVS 180
           SHI+CKACISRF DCPLCGADIEKIEA A+LQ TVDRFIEGHARIKR QV+SDKEQE+VS
Sbjct: 121 SHIYCKACISRFKDCPLCGADIEKIEAHADLQGTVDRFIEGHARIKRSQVNSDKEQEEVS 180

Query: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCS 240
           ESKVVIYEDVSMERGAFLIQQAMRAFRAQN  SAKSRLT+CVEDIRDQLERMG+SSELCS
Sbjct: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNFESAKSRLTICVEDIRDQLERMGNSSELCS 240

Query: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYY 300
           QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEK+HE+THTLSVSLNKIGDLKYY
Sbjct: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKSHEVTHTLSVSLNKIGDLKYY 300

Query: 301 EGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVS 360
           EGDLQAARSYY RSLNVRQDASKHHPDDP+Q L                       VAVS
Sbjct: 301 EGDLQAARSYYFRSLNVRQDASKHHPDDPSQILD----------------------VAVS 360

Query: 361 LAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 420
           LAKVADVDS LGNEDMAV+ FQEGIKLLESLTLNSENPGL+QRR+SVLKFLEGQL ERQK
Sbjct: 361 LAKVADVDSGLGNEDMAVNGFQEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLTERQK 398

BLAST of Lsi04G007600 vs. NCBI nr
Match: XP_022957022.1 (protein NCA1-like [Cucurbita moschata])

HSP 1 Score: 684.1 bits (1764), Expect = 7.7e-193
Identity = 362/425 (85.18%), Postives = 373/425 (87.76%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQGESGCPFAKSGRSDDASSRKPQTEAACPFSKSVRSDDA 60
           MTPVCP VKSARPDDASSRK QGES CPFAKSG SDDASSRK Q+EAACPFSKS RSDDA
Sbjct: 1   MTPVCPSVKSARPDDASSRKQQGESVCPFAKSGCSDDASSRKQQSEAACPFSKSARSDDA 60

Query: 61  SSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120
           S  K+  E E  G     ADAA  GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC
Sbjct: 61  SLTKSPVEGEKGG-----ADAASAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPC 120

Query: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQEKVS 180
           SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQV+SDKE E+VS
Sbjct: 121 SHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVNSDKENEEVS 180

Query: 181 ESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSSELCS 240
           ESKVVIYED+SMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMG+SSELCS
Sbjct: 181 ESKVVIYEDLSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGNSSELCS 240

Query: 241 QLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLKYY 300
           QLGAVLGTLGDCCRAAGDASSAI HFEESVEFLSKLPEKT EI  TLSVSLNKIGDLKYY
Sbjct: 241 QLGAVLGTLGDCCRAAGDASSAIIHFEESVEFLSKLPEKTREIIQTLSVSLNKIGDLKYY 300

Query: 301 EGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNHVAVS 360
           EGDLQAARSYY+RSLNVRQD SKHHPDDPAQ L                       VAVS
Sbjct: 301 EGDLQAARSYYLRSLNVRQDWSKHHPDDPAQILD----------------------VAVS 360

Query: 361 LAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQLAERQK 420
           LAKVADVDS LGNEDMAVDRFQEGIKLLESLTLNSENPGL+QRR+SVLKFLEGQ AERQK
Sbjct: 361 LAKVADVDSGLGNEDMAVDRFQEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQFAERQK 398

Query: 421 PSQSS 426
           P++SS
Sbjct: 421 PNRSS 398

BLAST of Lsi04G007600 vs. TAIR 10
Match: AT3G54360.1 (zinc ion binding )

HSP 1 Score: 489.2 bits (1258), Expect = 3.4e-138
Identity = 267/419 (63.72%), Postives = 313/419 (74.70%), Query Frame = 0

Query: 2   TPVCPFVKSARPDDASSRKHQGE---SGCPFAKSGRSDDASSRK--PQTEAACPFSKSVR 61
           T VCPF K+ARPDD S+RK QGE   SGCPF+K+ R DDAS+RK    T + CPFSKS R
Sbjct: 4   TSVCPFSKAARPDDGSTRK-QGEITASGCPFSKAARPDDASARKQGETTASGCPFSKSAR 63

Query: 62  SDDASSRKNQAEAESNGAEKDIADAAGTGGKCPFGYDSQTFKIGPLSCMICQALLFECSR 121
            D+  S K   E E N   KD  D+A    KCPFGYDSQTFK+GP SCM+CQALL+E SR
Sbjct: 64  PDENGS-KGCPEQEGN-LNKDSTDSATVPAKCPFGYDSQTFKLGPFSCMLCQALLYESSR 123

Query: 122 CVPCSHIFCKACISRFNDCPLCGADIEKIEADANLQATVDRFIEGHARIKRPQVSSDKEQ 181
           CVPC+H+FCK C++RF DCPLCGADIE IE D NLQ  VD+FIEGHARIKR  V+  +++
Sbjct: 124 CVPCTHVFCKVCLTRFKDCPLCGADIESIEVDENLQKMVDQFIEGHARIKRSVVNGTEKE 183

Query: 182 EKVSESKVVIYEDVSMERGAFLIQQAMRAFRAQNIGSAKSRLTVCVEDIRDQLERMGSSS 241
           E  +++K VIY DVSMERG+FL+QQAMRAF AQN  SAKSRL +C EDIRDQL R G++ 
Sbjct: 184 EVENDNKKVIYADVSMERGSFLVQQAMRAFSAQNYESAKSRLAMCTEDIRDQLGREGNTP 243

Query: 242 ELCSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGD 301
           ELCSQLGAVLG LGDC RA GD+SSA+KHFEESVEFL KLP    EITHTLSVSLNKIGD
Sbjct: 244 ELCSQLGAVLGMLGDCSRAMGDSSSAVKHFEESVEFLMKLPLNDLEITHTLSVSLNKIGD 303

Query: 302 LKYYEGDLQAARSYYIRSLNVRQDASKHHPDDPAQCLSNSYDTLVLYKLTQNEVKLIVNH 361
           LKYY+ DLQAARSYY R+LNVR+DA KHHP+ P+Q L                       
Sbjct: 304 LKYYDEDLQAARSYYDRALNVRRDAMKHHPNAPSQILD---------------------- 363

Query: 362 VAVSLAKVADVDSSLGNEDMAVDRFQEGIKLLESLTLNSENPGLQQRRESVLKFLEGQL 416
           VAVSLAKVAD+D +L NE  A D F+EG++LLESL L+SE+  L+QRR SVL+FL+ Q+
Sbjct: 364 VAVSLAKVADIDRTLQNEVAATDGFKEGMRLLESLKLDSEDSALEQRRLSVLEFLKKQV 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2V14.8e-13763.72Protein NCA1 OS=Arabidopsis thaliana OX=3702 GN=NCA1 PE=1 SV=1[more]
A5WW084.3e-0532.29E3 ubiquitin-protein ligase CHFR OS=Danio rerio OX=7955 GN=chfr PE=2 SV=1[more]
Q810L37.4e-0532.26E3 ubiquitin-protein ligase CHFR OS=Mus musculus OX=10090 GN=Chfr PE=1 SV=1[more]
Q5FWP42.8e-0422.08E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KPN84.2e-20588.26RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G581690 P... [more]
A0A5D3CV762.1e-20488.03Binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00322... [more]
A0A1S3BEN72.1e-20488.03uncharacterized protein LOC103488802 OS=Cucumis melo OX=3656 GN=LOC103488802 PE=... [more]
A0A6J1CBV44.2e-19785.95protein NCA1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010138 PE=4 SV=1[more]
A0A6J1GY053.7e-19385.18protein NCA1-like OS=Cucurbita moschata OX=3662 GN=LOC111458517 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038893051.11.2e-20991.06protein NCA1 [Benincasa hispida] >XP_038893052.1 protein NCA1 [Benincasa hispida... [more]
XP_004147090.18.7e-20588.26protein NCA1 [Cucumis sativus] >KGN51585.1 hypothetical protein Csa_009177 [Cucu... [more]
XP_008445920.14.3e-20488.03PREDICTED: uncharacterized protein LOC103488802 [Cucumis melo] >KAA0034103.1 bin... [more]
XP_022139165.18.8e-19785.95protein NCA1 isoform X1 [Momordica charantia] >XP_022139166.1 protein NCA1 isofo... [more]
XP_022957022.17.7e-19385.18protein NCA1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G54360.13.4e-13863.72zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 57..77
NoneNo IPR availablePFAMPF13424TPR_12coord: 244..319
e-value: 2.0E-8
score: 34.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..78
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..75
NoneNo IPR availablePANTHERPTHR15315:SF89PROTEIN NCA1coord: 1..418
NoneNo IPR availablePANTHERPTHR15315RING FINGER PROTEIN 41, 151coord: 1..418
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 99..145
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 192..394
e-value: 1.4E-12
score: 48.9
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 239..393
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 100..185
e-value: 2.7E-9
score: 38.8
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 120..129
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 104..138
score: 11.486073

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G007600.1Lsi04G007600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902553 positive regulation of catalase activity
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity