Lsi04G007530 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G007530 vs. ExPASy Swiss-Prot
Match: F4I1T7 (Nuclear pore complex protein NUP214 OS=Arabidopsis thaliana OX=3702 GN=NUP214 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 3.4e-46
Identity = 249/671 (37.11%), Postives = 333/671 (49.63%), Query Frame = 0

Query: 18   SLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFTEKSKASDFFSVTRSDSV--- 77
            S+Q+ + T  S  S F+SS     K     FS +S         +   F    S S+   
Sbjct: 1185 SVQRLSTT--SSGSDFESS-----KGFGAQFSTMSSGAPASSFSSKSLFGFNSSSSIPGD 1244

Query: 78   ---------QKSNINLDQKSFIFT-----ISSKQTPTLKDSIDTSNSDNQKTANAKERHT 137
                       S   LD  S +FT     +SS     +  SI  S++   +T +     T
Sbjct: 1245 KFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQDPVPASIPISSAPVPQTFSVTSTST 1304

Query: 138  TTSP----LFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNT 197
             ++      FG         +   +   P+           +L  +SPS P      +++
Sbjct: 1305 VSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGPTAGFTFNLPALSPSSP----EMVSS 1364

Query: 198  TSSSSTLFSGFA----VSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 257
            ++  S+LF   A    VS    S+ + +  +  L +ST L+ S+P ++  D+ FQ+P++ 
Sbjct: 1365 STGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTSLS-STPPITPPDA-FQSPQVS 1424

Query: 258  STSPTLSSLNPSLEPLKKEL---PILKSDDDTEKQTPASKPESYELKFQ-------PSVT 317
            + S  +    P  EP K E     IL +    +    A+K ++  L  +        +VT
Sbjct: 1425 TPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVT 1484

Query: 318  PADKNHVEP--TSKTQTVAKDVGGQVPNVIGNAQPQQPSVAFASPNLTSKIFANGRNEAS 377
            P   +      +S TQ+    +     +  G++QPQQ S   A    +S   A+   E  
Sbjct: 1485 PVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSSTPAPFPASSPTSASPFGEKK 1544

Query: 378  NTAVTQDDDMDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMT 437
            +   TQ+D+MDEEAPE +   E ++ S GGFG  STP  GAPK NPFGGPFG  NAT+ T
Sbjct: 1545 DIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPNPGAPKTNPFGGPFG--NATTTT 1604

Query: 438  SSFTMASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMAT--QAPSQGGFG 497
            S+    + PSGELF+PASF+FQ+P  SQ A           GFGS   T  Q P+Q GFG
Sbjct: 1605 SNPFNMTVPSGELFKPASFNFQNPQPSQPA-----------GFGSFSVTPSQTPAQSGFG 1664

Query: 498  QPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGS 557
            QP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP GF G             +G  +
Sbjct: 1665 QPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPTGFGGSNPG---------SGLPN 1724

Query: 558  SVGSGGFAGVGSGGGGGFGGVGSGGGGFAGTVST-GGGFAGASSTTGGFAAAA----GGG 617
            +  SGGFA  GS   GGF  + S G GFAG  ST  GGFA  +S +GGFA AA    GGG
Sbjct: 1725 APASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSGGFAGAAPGGGGGG 1784

Query: 618  F--VGAAGGGFAGAA----GGGFAGAA--GGFGAFG----SQQGSGGFSAFGAGGAGGTG 633
            F  +G+  GGF G A     GGFAGAA  GGFG FG     Q G GGFSAFG G +G TG
Sbjct: 1785 FGGLGSGTGGFGGFAPQGSSGGFAGAAGGGGFGGFGGQAQGQAGGGGFSAFG-GNSGATG 1819

BLAST of Lsi04G007530 vs. ExPASy TrEMBL
Match: A0A5D3CWG0 (Nuclear pore complex protein NUP214 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003090 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 3.1e-236
Identity = 511/647 (78.98%), Postives = 538/647 (83.15%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MWAS LQPSNTSRQKS  LQK NAT PSP  VFQSSH+MLKK+NN A S  SENKFT   
Sbjct: 786  MWASVLQPSNTSRQKSLPLQKTNATAPSPPPVFQSSHDMLKKNNNAAHSATSENKFTDMA 845

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKS IN+DQKS IFTISSKQTP  +DSI TSN DNQKTAN 
Sbjct: 846  CPEKSKASDFFSATRSDSVQKSKINVDQKSSIFTISSKQTPPPEDSIGTSNVDNQKTANV 905

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTS LFGSA+KPES  VGTM SLVPTVD ARK EEK+S+TTIS SV AP  A LN
Sbjct: 906  KERHTTTSQLFGSANKPESPFVGTMPSLVPTVDGARKTEEKKSVTTISQSVSAP--APLN 965

Query: 181  TTSSSSTLFSGFAVSKPLPSS---AAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 240
            T+SS+STLFSGFAVSK LPSS   AAV+DLNQP STSTQLNF SPVVS S+SLFQAPK V
Sbjct: 966  TSSSASTLFSGFAVSKSLPSSAAVAAVVDLNQPQSTSTQLNF-SPVVSGSNSLFQAPK-V 1025

Query: 241  STSPTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPT 300
             TSPTLSSLNP++E  K EL +LKS+DD EKQT +SKP S+ELKFQPS+TPADKNHVEPT
Sbjct: 1026 PTSPTLSSLNPTMESSKTELSVLKSNDDAEKQTLSSKPGSHELKFQPSITPADKNHVEPT 1085

Query: 301  SKTQTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDD 360
            SKTQTV KDVGGQVPNV+G+AQ QQPSVAFA   S NLTSKIFAN RNE SN  VTQDDD
Sbjct: 1086 SKTQTVFKDVGGQVPNVVGDAQAQQPSVAFASIPSQNLTSKIFANSRNETSNAVVTQDDD 1145

Query: 361  MDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPP 420
            MDEEAPETNNNVEFNLSSLGGFGNSSTP+SGAPKPNPFGGPFGNVNA S+T+SF MASPP
Sbjct: 1146 MDEEAPETNNNVEFNLSSLGGFGNSSTPISGAPKPNPFGGPFGNVNAASVTTSFNMASPP 1205

Query: 421  SGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQA 480
            SGELFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ATQAP QGGFGQPAQIGVGQQA
Sbjct: 1206 SGELFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVATQAPPQGGFGQPAQIGVGQQA 1265

Query: 481  LGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVG 540
            LGNVLGSFGQSRQLGP+LPGTG+GSPGGFSGGFT+ KPVGV            GGFAGVG
Sbjct: 1266 LGNVLGSFGQSRQLGPTLPGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVG 1325

Query: 541  SGGGGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGG 600
            SGGGGGFGGV    GGFAG  STGGGFAGASSTT                GGFAGAAGGG
Sbjct: 1326 SGGGGGFGGV----GGFAGAASTGGGFAGASSTT----------------GGFAGAAGGG 1385

Query: 601  FAGAAGGFGAFGSQQGS-GGFSAFG---AGGAGGTGKPPELFTQIRK 633
            F G AGGFGAFGSQQ S GGFSAFG   AGGA  TGKPPELFTQIRK
Sbjct: 1386 FGGTAGGFGAFGSQQVSGGGFSAFGTAAAGGASVTGKPPELFTQIRK 1396

BLAST of Lsi04G007530 vs. ExPASy TrEMBL
Match: A0A5A7SY34 (Nuclear pore complex protein NUP214 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001060 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 3.1e-236
Identity = 511/647 (78.98%), Postives = 538/647 (83.15%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MWAS LQPSNTSRQKS  LQK NAT PSP  VFQSSH+MLKK+NN A S  SENKFT   
Sbjct: 1077 MWASVLQPSNTSRQKSLPLQKTNATAPSPPPVFQSSHDMLKKNNNAAHSATSENKFTDMA 1136

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKS IN+DQKS IFTISSKQTP  +DSI TSN DNQKTAN 
Sbjct: 1137 CPEKSKASDFFSATRSDSVQKSKINVDQKSSIFTISSKQTPPPEDSIGTSNVDNQKTANV 1196

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTS LFGSA+KPES  VGTM SLVPTVD ARK EEK+S+TTIS SV AP  A LN
Sbjct: 1197 KERHTTTSQLFGSANKPESPFVGTMPSLVPTVDGARKTEEKKSVTTISQSVSAP--APLN 1256

Query: 181  TTSSSSTLFSGFAVSKPLPSS---AAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 240
            T+SS+STLFSGFAVSK LPSS   AAV+DLNQP STSTQLNF SPVVS S+SLFQAPK V
Sbjct: 1257 TSSSASTLFSGFAVSKSLPSSAAVAAVVDLNQPQSTSTQLNF-SPVVSGSNSLFQAPK-V 1316

Query: 241  STSPTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPT 300
             TSPTLSSLNP++E  K EL +LKS+DD EKQT +SKP S+ELKFQPS+TPADKNHVEPT
Sbjct: 1317 PTSPTLSSLNPTMESSKTELSVLKSNDDAEKQTLSSKPGSHELKFQPSITPADKNHVEPT 1376

Query: 301  SKTQTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDD 360
            SKTQTV KDVGGQVPNV+G+AQ QQPSVAFA   S NLTSKIFAN RNE SN  VTQDDD
Sbjct: 1377 SKTQTVFKDVGGQVPNVVGDAQAQQPSVAFASIPSQNLTSKIFANSRNETSNAVVTQDDD 1436

Query: 361  MDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPP 420
            MDEEAPETNNNVEFNLSSLGGFGNSSTP+SGAPKPNPFGGPFGNVNA S+T+SF MASPP
Sbjct: 1437 MDEEAPETNNNVEFNLSSLGGFGNSSTPISGAPKPNPFGGPFGNVNAASVTTSFNMASPP 1496

Query: 421  SGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQA 480
            SGELFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ATQAP QGGFGQPAQIGVGQQA
Sbjct: 1497 SGELFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVATQAPPQGGFGQPAQIGVGQQA 1556

Query: 481  LGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVG 540
            LGNVLGSFGQSRQLGP+LPGTG+GSPGGFSGGFT+ KPVGV            GGFAGVG
Sbjct: 1557 LGNVLGSFGQSRQLGPTLPGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVG 1616

Query: 541  SGGGGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGG 600
            SGGGGGFGGV    GGFAG  STGGGFAGASSTT                GGFAGAAGGG
Sbjct: 1617 SGGGGGFGGV----GGFAGAASTGGGFAGASSTT----------------GGFAGAAGGG 1676

Query: 601  FAGAAGGFGAFGSQQGS-GGFSAFG---AGGAGGTGKPPELFTQIRK 633
            F G AGGFGAFGSQQ S GGFSAFG   AGGA  TGKPPELFTQIRK
Sbjct: 1677 FGGTAGGFGAFGSQQVSGGGFSAFGTAAAGGASVTGKPPELFTQIRK 1687

BLAST of Lsi04G007530 vs. ExPASy TrEMBL
Match: A0A0A0KV45 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G583270 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 7.6e-235
Identity = 510/688 (74.13%), Postives = 535/688 (77.76%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MW SPLQPSNTSRQKS  LQK N T PSP  VFQSSH+MLKK NNEA SV SENKFT   
Sbjct: 1051 MWNSPLQPSNTSRQKSLPLQKINVTPPSPPPVFQSSHDMLKKKNNEAHSVTSENKFTDVA 1110

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKSNIN+DQKS IFTISSKQ PT  DSI TSN DNQKTAN 
Sbjct: 1111 CPEKSKASDFFSATRSDSVQKSNINVDQKSSIFTISSKQMPTPIDSIATSNVDNQKTANV 1170

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTSP FGSA+KPES  VG+M SLVPTVD +RK EEK+S+TTIS SV AP  A LN
Sbjct: 1171 KERHTTTSPFFGSANKPESPFVGSMPSLVPTVDGSRKTEEKKSVTTISQSVSAP--APLN 1230

Query: 181  TTSSSSTLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTS 240
            T+SS+STLFSGFAVSK LPSSAAVIDLNQP STSTQLNFSSPVVS S+SLFQAPKIV TS
Sbjct: 1231 TSSSASTLFSGFAVSKALPSSAAVIDLNQPPSTSTQLNFSSPVVSSSNSLFQAPKIVPTS 1290

Query: 241  PTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKT 300
            PTLSSLNP+LE  K EL + KS+DD E+Q  +SKP S+ELKFQPS+TPADKNHVEPTSKT
Sbjct: 1291 PTLSSLNPTLESSKTELSVPKSNDDAEEQILSSKPGSHELKFQPSITPADKNHVEPTSKT 1350

Query: 301  QTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDE 360
            QTV KDVGGQ  NV+GNAQPQQPSVAFA   SPNLTSKIFAN RNE SN  VTQDDDMDE
Sbjct: 1351 QTVFKDVGGQDSNVVGNAQPQQPSVAFASIPSPNLTSKIFANSRNETSNAVVTQDDDMDE 1410

Query: 361  EAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGE 420
            EAPETNNNVEFNLSSLGGFGNSSTP+SG PKPNPFGGPFGNVNA SMTSSF MASPPSGE
Sbjct: 1411 EAPETNNNVEFNLSSLGGFGNSSTPISGGPKPNPFGGPFGNVNAASMTSSFNMASPPSGE 1470

Query: 421  LFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGN 480
            LFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ TQ PSQGGFGQP+QIGVGQQALGN
Sbjct: 1471 LFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVPTQPPSQGGFGQPSQIGVGQQALGN 1530

Query: 481  VLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGG 540
            VLGSFGQSRQLGP++ GTG+GSPGGFSGGFT+ KPVGV            GGFAGVGSGG
Sbjct: 1531 VLGSFGQSRQLGPTVHGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVGSGG 1590

Query: 541  GGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAG 600
            GGGFGGV    GGFAG  STGGGFAGASST                 GGFAGAAGGGF G
Sbjct: 1591 GGGFGGV----GGFAGAASTGGGFAGASST----------------AGGFAGAAGGGFGG 1650

Query: 601  AAGGFGAFGSQQGSGGFSAFG--------------------------------------- 633
             AGGFGAFGSQQ SGGFSAFG                                       
Sbjct: 1651 TAGGFGAFGSQQVSGGFSAFGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1704

BLAST of Lsi04G007530 vs. ExPASy TrEMBL
Match: A0A1S3BDU8 (LOW QUALITY PROTEIN: nuclear pore complex protein NUP214 OS=Cucumis melo OX=3656 GN=LOC103488807 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 2.1e-229
Identity = 501/646 (77.55%), Postives = 530/646 (82.04%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKF---- 60
            MWAS LQPSNTSRQKS  LQK NAT PSP  VFQSSH+MLKK   +    + +       
Sbjct: 1051 MWASVLQPSNTSRQKSLPLQKTNATAPSPPPVFQSSHDMLKKIIMQLTVRLQKTNLRTWH 1110

Query: 61   TEKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANAK 120
             EKSKASDFFS TRSDSVQKS IN+DQKS IFTISSKQTP  +DSI TSN DNQKTAN K
Sbjct: 1111 PEKSKASDFFSATRSDSVQKSKINVDQKSSIFTISSKQTPPPEDSIGTSNVDNQKTANVK 1170

Query: 121  ERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNT 180
            ERHTTTS LFGSA+KPES  VGTM SLVPTVD ARK EEK+S+TTIS SV AP  A LNT
Sbjct: 1171 ERHTTTSQLFGSANKPESPFVGTMPSLVPTVDGARKTEEKKSVTTISQSVSAP--APLNT 1230

Query: 181  TSSSSTLFSGFAVSKPLPSS---AAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVS 240
            +SS+STLFSGFAVSK LPSS   AAV+DLNQP STSTQLNF SPVVS S+SLFQAPK V 
Sbjct: 1231 SSSASTLFSGFAVSKSLPSSAAVAAVVDLNQPQSTSTQLNF-SPVVSGSNSLFQAPK-VP 1290

Query: 241  TSPTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTS 300
            TSPTLSSLNP++E  K EL +LKS+DD EKQT +SKP S+ELKFQPS+TPADKNHVEPTS
Sbjct: 1291 TSPTLSSLNPTMESSKTELSVLKSNDDAEKQTLSSKPGSHELKFQPSITPADKNHVEPTS 1350

Query: 301  KTQTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDM 360
            KTQTV KDVGGQVPNV+G+AQ QQPSVAFA   S NLTSKIFAN RNE SN  VTQDDDM
Sbjct: 1351 KTQTVFKDVGGQVPNVVGDAQAQQPSVAFASIPSQNLTSKIFANSRNETSNAVVTQDDDM 1410

Query: 361  DEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPS 420
            DEEAPETNNNVEFNLSSLGGFGNSSTP+SGAPKPNPFGGPFGNVNA S+T+SF MASPPS
Sbjct: 1411 DEEAPETNNNVEFNLSSLGGFGNSSTPISGAPKPNPFGGPFGNVNAASVTTSFNMASPPS 1470

Query: 421  GELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQAL 480
            GELFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ATQAP QGGFGQPAQIGVGQQAL
Sbjct: 1471 GELFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVATQAPPQGGFGQPAQIGVGQQAL 1530

Query: 481  GNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGS 540
            GNVLGSFGQSRQLGP+LPGTG+GSPGGFSGGFT+ KPVGV            GGFAGVGS
Sbjct: 1531 GNVLGSFGQSRQLGPTLPGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVGS 1590

Query: 541  GGGGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGF 600
            GGGGGFGGV    GGFAG  STGGGFAGASSTT                GGFAGAAGGGF
Sbjct: 1591 GGGGGFGGV----GGFAGAASTGGGFAGASSTT----------------GGFAGAAGGGF 1650

Query: 601  AGAAGGFGAFGSQQGS-GGFSAFG---AGGAGGTGKPPELFTQIRK 633
             G AGGFGAFGSQQ S GGFSAFG   AGGA  TGKPPELFTQIRK
Sbjct: 1651 GGTAGGFGAFGSQQVSGGGFSAFGTAAAGGASVTGKPPELFTQIRK 1660

BLAST of Lsi04G007530 vs. ExPASy TrEMBL
Match: A0A6J1G030 (nuclear pore complex protein NUP214 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449495 PE=4 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 4.2e-217
Identity = 474/641 (73.95%), Postives = 516/641 (80.50%), Query Frame = 0

Query: 3    ASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT----- 62
            A+P   ++  RQK Q LQK N T PS L VFQSSHEM+KKSN+EA+S  SENKF      
Sbjct: 1055 ATPFSWASPPRQKFQPLQKTNGTAPSSLPVFQSSHEMVKKSNSEAYSAASENKFAEVTYP 1114

Query: 63   EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANAKE 122
            EKSKASDFFS+ RSDSVQKSN+N +QKS IF  SSK   T KDSI+T N ++QKTAN KE
Sbjct: 1115 EKSKASDFFSLARSDSVQKSNMNFEQKSSIFVTSSKPMSTPKDSIETLNPNSQKTANVKE 1174

Query: 123  RHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNTT 182
            R TT SPLFGSA+KPE  SVGT SSLVPTVD  RK EEK+  T  SPSVPAP  A +NT 
Sbjct: 1175 RLTTPSPLFGSANKPEPVSVGTTSSLVPTVDGLRKTEEKKPPTVFSPSVPAP--APVNTP 1234

Query: 183  SSSSTLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTSPT 242
             S+STLFSG  +SK  PS AAV+DLN+PLSTSTQ +F+SPVVS+SDSLFQAPK+VS    
Sbjct: 1235 PSASTLFSGSPLSKSFPSPAAVVDLNKPLSTSTQSSFASPVVSVSDSLFQAPKMVSPPSN 1294

Query: 243  LSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKTQT 302
            LSSLNP+L    KE P+ KSD DTEKQ PASKPES ELK QPSVT A  NHVEPTS TQT
Sbjct: 1295 LSSLNPTLVSSSKEQPMPKSDADTEKQAPASKPESRELKLQPSVTLAVGNHVEPTSVTQT 1354

Query: 303  VAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDEEA 362
            V+KDVGG VP V+ +AQPQQ   AF    +PN TSK  ANG++E S+  +TQDDDMDEEA
Sbjct: 1355 VSKDVGGHVPFVVADAQPQQSPAAFVPLPTPNSTSKAAANGKSETSDALITQDDDMDEEA 1414

Query: 363  PETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGELF 422
            PET NNVEF+LSSLGGFG +STPMS APKPNPFGG FGNVNATSM SSFT ASPPSGELF
Sbjct: 1415 PET-NNVEFSLSSLGGFGTTSTPMSNAPKPNPFGGSFGNVNATSMNSSFTTASPPSGELF 1474

Query: 423  RPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVL 482
            RPASFSFQSPLASQAASQPTN+VAFSG FGS MATQAP+QGGFGQPAQIGVGQQALG VL
Sbjct: 1475 RPASFSFQSPLASQAASQPTNAVAFSGSFGSGMATQAPAQGGFGQPAQIGVGQQALGTVL 1534

Query: 483  GSFGQSRQLGPSLPGTGTGSPGGFS-GGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGGG 542
            GSFGQSRQLGPSLPGT +GSPGGF+ GGFTS+KPVG             GGFAGVGSGGG
Sbjct: 1535 GSFGQSRQLGPSLPGTASGSPGGFNGGGFTSVKPVG-------------GGFAGVGSGGG 1594

Query: 543  GGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAGA 602
            GGFGG G  GGGFAG  STGGGFAGAS  TGGFA A GGGF GAAGGGFAGAAGGGFAGA
Sbjct: 1595 GGFGGGGFSGGGFAGAASTGGGFAGASPPTGGFAGATGGGFAGAAGGGFAGAAGGGFAGA 1654

Query: 603  AGGFGAFGSQQGSGGFSAFGA--GGAGGTGKPPELFTQIRK 633
            AGGFGAFG+QQGSGGFSAFGA  GG+GGTGKPPELFTQIRK
Sbjct: 1655 AGGFGAFGNQQGSGGFSAFGAAPGGSGGTGKPPELFTQIRK 1679

BLAST of Lsi04G007530 vs. NCBI nr
Match: XP_038892124.1 (nuclear pore complex protein NUP214 isoform X2 [Benincasa hispida])

HSP 1 Score: 936.0 bits (2418), Expect = 1.7e-268
Identity = 554/637 (86.97%), Postives = 575/637 (90.27%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFTEKS 60
            MWASPLQPSN SRQKSQ LQK NAT PS LSVFQSSHEMLKKSNNEAFSV SENKFTEKS
Sbjct: 1054 MWASPLQPSNISRQKSQPLQKTNATAPS-LSVFQSSHEMLKKSNNEAFSVTSENKFTEKS 1113

Query: 61   KASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANAKERHT 120
            KASDFFSVTR+DSVQKSN NLD+K  IFTISSKQ  T KD IDTSN DNQKTAN+KERHT
Sbjct: 1114 KASDFFSVTRTDSVQKSNTNLDKKPSIFTISSKQMATPKDFIDTSNLDNQKTANSKERHT 1173

Query: 121  TTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNTTSSS 180
            TTSPLFGSA+KPESASVGTMSSLVPTVDEARK  EKRSL TISPSVPAP  AR N+ SSS
Sbjct: 1174 TTSPLFGSANKPESASVGTMSSLVPTVDEARK--EKRSLKTISPSVPAPTPARFNSPSSS 1233

Query: 181  STLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTSPTLSS 240
            STLFSGFAVSKPLPSSAA IDLNQPLSTSTQLNFSSPVVS+SDSLFQA K+VSTS TLSS
Sbjct: 1234 STLFSGFAVSKPLPSSAAAIDLNQPLSTSTQLNFSSPVVSVSDSLFQATKMVSTSSTLSS 1293

Query: 241  LNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKTQTVAK 300
            LNP LE  KKELP+ KS+ DTEK+TPASKPES+ELKFQPS+TPA+KNH+EPTSKTQTV K
Sbjct: 1294 LNPILESSKKELPVSKSEGDTEKKTPASKPESHELKFQPSITPANKNHLEPTSKTQTVPK 1353

Query: 301  DVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDEEAPET 360
            DVGGQ+PNVIG+AQPQ PSVAFA   SPNLTSK   NGRNE SN  VTQDDDMDEEAPET
Sbjct: 1354 DVGGQIPNVIGDAQPQPPSVAFASLPSPNLTSKTSGNGRNETSNVTVTQDDDMDEEAPET 1413

Query: 361  NNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGELFRPA 420
             NNVEFNLS LGGFGNSS+PMSGAPKPNPFGG FGNVNATSMTSSFTMASPPSGELFRPA
Sbjct: 1414 INNVEFNLSGLGGFGNSSSPMSGAPKPNPFGGSFGNVNATSMTSSFTMASPPSGELFRPA 1473

Query: 421  SFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF 480
            SFSFQSPLASQAAS PTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF
Sbjct: 1474 SFSFQSPLASQAASLPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF 1533

Query: 481  GQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGGGGGFG 540
            GQSRQLGPSLPGTG+GS G FSGGFT +KPV VGGFAG GSS GSGGFAGVGSGGGGGFG
Sbjct: 1534 GQSRQLGPSLPGTGSGSLGSFSGGFTGMKPVAVGGFAGVGSSGGSGGFAGVGSGGGGGFG 1593

Query: 541  GVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAGAAGGF 600
            GVGS  GGFAGT+STGGGFAGAS+TTGGFA        GAAGGGFAGAAGGGFAGAAGGF
Sbjct: 1594 GVGSAAGGFAGTISTGGGFAGASATTGGFA--------GAAGGGFAGAAGGGFAGAAGGF 1653

Query: 601  GAFGSQQGSGGFSAFG--AGGAGGTGKPPELFTQIRK 633
            GAFGSQQGSGGFSAFG  AGGAGGTGKPPELFTQIRK
Sbjct: 1654 GAFGSQQGSGGFSAFGAAAGGAGGTGKPPELFTQIRK 1679

BLAST of Lsi04G007530 vs. NCBI nr
Match: XP_038892123.1 (nuclear pore complex protein NUP214 isoform X1 [Benincasa hispida])

HSP 1 Score: 936.0 bits (2418), Expect = 1.7e-268
Identity = 554/637 (86.97%), Postives = 575/637 (90.27%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFTEKS 60
            MWASPLQPSN SRQKSQ LQK NAT PS LSVFQSSHEMLKKSNNEAFSV SENKFTEKS
Sbjct: 1059 MWASPLQPSNISRQKSQPLQKTNATAPS-LSVFQSSHEMLKKSNNEAFSVTSENKFTEKS 1118

Query: 61   KASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANAKERHT 120
            KASDFFSVTR+DSVQKSN NLD+K  IFTISSKQ  T KD IDTSN DNQKTAN+KERHT
Sbjct: 1119 KASDFFSVTRTDSVQKSNTNLDKKPSIFTISSKQMATPKDFIDTSNLDNQKTANSKERHT 1178

Query: 121  TTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNTTSSS 180
            TTSPLFGSA+KPESASVGTMSSLVPTVDEARK  EKRSL TISPSVPAP  AR N+ SSS
Sbjct: 1179 TTSPLFGSANKPESASVGTMSSLVPTVDEARK--EKRSLKTISPSVPAPTPARFNSPSSS 1238

Query: 181  STLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTSPTLSS 240
            STLFSGFAVSKPLPSSAA IDLNQPLSTSTQLNFSSPVVS+SDSLFQA K+VSTS TLSS
Sbjct: 1239 STLFSGFAVSKPLPSSAAAIDLNQPLSTSTQLNFSSPVVSVSDSLFQATKMVSTSSTLSS 1298

Query: 241  LNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKTQTVAK 300
            LNP LE  KKELP+ KS+ DTEK+TPASKPES+ELKFQPS+TPA+KNH+EPTSKTQTV K
Sbjct: 1299 LNPILESSKKELPVSKSEGDTEKKTPASKPESHELKFQPSITPANKNHLEPTSKTQTVPK 1358

Query: 301  DVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDEEAPET 360
            DVGGQ+PNVIG+AQPQ PSVAFA   SPNLTSK   NGRNE SN  VTQDDDMDEEAPET
Sbjct: 1359 DVGGQIPNVIGDAQPQPPSVAFASLPSPNLTSKTSGNGRNETSNVTVTQDDDMDEEAPET 1418

Query: 361  NNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGELFRPA 420
             NNVEFNLS LGGFGNSS+PMSGAPKPNPFGG FGNVNATSMTSSFTMASPPSGELFRPA
Sbjct: 1419 INNVEFNLSGLGGFGNSSSPMSGAPKPNPFGGSFGNVNATSMTSSFTMASPPSGELFRPA 1478

Query: 421  SFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF 480
            SFSFQSPLASQAAS PTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF
Sbjct: 1479 SFSFQSPLASQAASLPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGNVLGSF 1538

Query: 481  GQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGGGGGFG 540
            GQSRQLGPSLPGTG+GS G FSGGFT +KPV VGGFAG GSS GSGGFAGVGSGGGGGFG
Sbjct: 1539 GQSRQLGPSLPGTGSGSLGSFSGGFTGMKPVAVGGFAGVGSSGGSGGFAGVGSGGGGGFG 1598

Query: 541  GVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAGAAGGF 600
            GVGS  GGFAGT+STGGGFAGAS+TTGGFA        GAAGGGFAGAAGGGFAGAAGGF
Sbjct: 1599 GVGSAAGGFAGTISTGGGFAGASATTGGFA--------GAAGGGFAGAAGGGFAGAAGGF 1658

Query: 601  GAFGSQQGSGGFSAFG--AGGAGGTGKPPELFTQIRK 633
            GAFGSQQGSGGFSAFG  AGGAGGTGKPPELFTQIRK
Sbjct: 1659 GAFGSQQGSGGFSAFGAAAGGAGGTGKPPELFTQIRK 1684

BLAST of Lsi04G007530 vs. NCBI nr
Match: XP_031741375.1 (nuclear pore complex protein NUP214 isoform X2 [Cucumis sativus])

HSP 1 Score: 839.0 bits (2166), Expect = 2.8e-239
Identity = 510/647 (78.83%), Postives = 535/647 (82.69%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MW SPLQPSNTSRQKS  LQK N T PSP  VFQSSH+MLKK NNEA SV SENKFT   
Sbjct: 1041 MWNSPLQPSNTSRQKSLPLQKINVTPPSPPPVFQSSHDMLKKKNNEAHSVTSENKFTDVA 1100

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKSNIN+DQKS IFTISSKQ PT  DSI TSN DNQKTAN 
Sbjct: 1101 CPEKSKASDFFSATRSDSVQKSNINVDQKSSIFTISSKQMPTPIDSIATSNVDNQKTANV 1160

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTSP FGSA+KPES  VG+M SLVPTVD +RK EEK+S+TTIS SV AP  A LN
Sbjct: 1161 KERHTTTSPFFGSANKPESPFVGSMPSLVPTVDGSRKTEEKKSVTTISQSVSAP--APLN 1220

Query: 181  TTSSSSTLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTS 240
            T+SS+STLFSGFAVSK LPSSAAVIDLNQP STSTQLNFSSPVVS S+SLFQAPKIV TS
Sbjct: 1221 TSSSASTLFSGFAVSKALPSSAAVIDLNQPPSTSTQLNFSSPVVSSSNSLFQAPKIVPTS 1280

Query: 241  PTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKT 300
            PTLSSLNP+LE  K EL + KS+DD E+Q  +SKP S+ELKFQPS+TPADKNHVEPTSKT
Sbjct: 1281 PTLSSLNPTLESSKTELSVPKSNDDAEEQILSSKPGSHELKFQPSITPADKNHVEPTSKT 1340

Query: 301  QTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDE 360
            QTV KDVGGQ  NV+GNAQPQQPSVAFA   SPNLTSKIFAN RNE SN  VTQDDDMDE
Sbjct: 1341 QTVFKDVGGQDSNVVGNAQPQQPSVAFASIPSPNLTSKIFANSRNETSNAVVTQDDDMDE 1400

Query: 361  EAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGE 420
            EAPETNNNVEFNLSSLGGFGNSSTP+SG PKPNPFGGPFGNVNA SMTSSF MASPPSGE
Sbjct: 1401 EAPETNNNVEFNLSSLGGFGNSSTPISGGPKPNPFGGPFGNVNAASMTSSFNMASPPSGE 1460

Query: 421  LFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGN 480
            LFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ TQ PSQGGFGQP+QIGVGQQALGN
Sbjct: 1461 LFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVPTQPPSQGGFGQPSQIGVGQQALGN 1520

Query: 481  VLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGG 540
            VLGSFGQSRQLGP++ GTG+GSPGGFSGGFT+ KPVGV            GGFAGVGSGG
Sbjct: 1521 VLGSFGQSRQLGPTVHGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVGSGG 1580

Query: 541  GGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAG 600
            GGGFGGV    GGFAG  STGGGFAGASST                 GGFAGAAGGGF G
Sbjct: 1581 GGGFGGV----GGFAGAASTGGGFAGASST----------------AGGFAGAAGGGFGG 1640

Query: 601  AAGGFGAFGSQQGSGGFSAFG-------AGGAGGTGKPPELFTQIRK 633
             AGGFGAFGSQQ SGGFSAFG       AGGAG TGKPPELFTQIRK
Sbjct: 1641 TAGGFGAFGSQQVSGGFSAFGAAAAAAAAGGAGVTGKPPELFTQIRK 1653

BLAST of Lsi04G007530 vs. NCBI nr
Match: XP_031741374.1 (nuclear pore complex protein NUP214 isoform X1 [Cucumis sativus] >KGN52214.2 hypothetical protein Csa_008316 [Cucumis sativus])

HSP 1 Score: 839.0 bits (2166), Expect = 2.8e-239
Identity = 510/647 (78.83%), Postives = 535/647 (82.69%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MW SPLQPSNTSRQKS  LQK N T PSP  VFQSSH+MLKK NNEA SV SENKFT   
Sbjct: 1051 MWNSPLQPSNTSRQKSLPLQKINVTPPSPPPVFQSSHDMLKKKNNEAHSVTSENKFTDVA 1110

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKSNIN+DQKS IFTISSKQ PT  DSI TSN DNQKTAN 
Sbjct: 1111 CPEKSKASDFFSATRSDSVQKSNINVDQKSSIFTISSKQMPTPIDSIATSNVDNQKTANV 1170

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTSP FGSA+KPES  VG+M SLVPTVD +RK EEK+S+TTIS SV AP  A LN
Sbjct: 1171 KERHTTTSPFFGSANKPESPFVGSMPSLVPTVDGSRKTEEKKSVTTISQSVSAP--APLN 1230

Query: 181  TTSSSSTLFSGFAVSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIVSTS 240
            T+SS+STLFSGFAVSK LPSSAAVIDLNQP STSTQLNFSSPVVS S+SLFQAPKIV TS
Sbjct: 1231 TSSSASTLFSGFAVSKALPSSAAVIDLNQPPSTSTQLNFSSPVVSSSNSLFQAPKIVPTS 1290

Query: 241  PTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPTSKT 300
            PTLSSLNP+LE  K EL + KS+DD E+Q  +SKP S+ELKFQPS+TPADKNHVEPTSKT
Sbjct: 1291 PTLSSLNPTLESSKTELSVPKSNDDAEEQILSSKPGSHELKFQPSITPADKNHVEPTSKT 1350

Query: 301  QTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDDMDE 360
            QTV KDVGGQ  NV+GNAQPQQPSVAFA   SPNLTSKIFAN RNE SN  VTQDDDMDE
Sbjct: 1351 QTVFKDVGGQDSNVVGNAQPQQPSVAFASIPSPNLTSKIFANSRNETSNAVVTQDDDMDE 1410

Query: 361  EAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPPSGE 420
            EAPETNNNVEFNLSSLGGFGNSSTP+SG PKPNPFGGPFGNVNA SMTSSF MASPPSGE
Sbjct: 1411 EAPETNNNVEFNLSSLGGFGNSSTPISGGPKPNPFGGPFGNVNAASMTSSFNMASPPSGE 1470

Query: 421  LFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQALGN 480
            LFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ TQ PSQGGFGQP+QIGVGQQALGN
Sbjct: 1471 LFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVPTQPPSQGGFGQPSQIGVGQQALGN 1530

Query: 481  VLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVGSGG 540
            VLGSFGQSRQLGP++ GTG+GSPGGFSGGFT+ KPVGV            GGFAGVGSGG
Sbjct: 1531 VLGSFGQSRQLGPTVHGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVGSGG 1590

Query: 541  GGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGGFAG 600
            GGGFGGV    GGFAG  STGGGFAGASST                 GGFAGAAGGGF G
Sbjct: 1591 GGGFGGV----GGFAGAASTGGGFAGASST----------------AGGFAGAAGGGFGG 1650

Query: 601  AAGGFGAFGSQQGSGGFSAFG-------AGGAGGTGKPPELFTQIRK 633
             AGGFGAFGSQQ SGGFSAFG       AGGAG TGKPPELFTQIRK
Sbjct: 1651 TAGGFGAFGSQQVSGGFSAFGAAAAAAAAGGAGVTGKPPELFTQIRK 1663

BLAST of Lsi04G007530 vs. NCBI nr
Match: TYK15805.1 (nuclear pore complex protein NUP214 [Cucumis melo var. makuwa])

HSP 1 Score: 827.8 bits (2137), Expect = 6.4e-236
Identity = 511/647 (78.98%), Postives = 538/647 (83.15%), Query Frame = 0

Query: 1    MWASPLQPSNTSRQKSQSLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFT--- 60
            MWAS LQPSNTSRQKS  LQK NAT PSP  VFQSSH+MLKK+NN A S  SENKFT   
Sbjct: 786  MWASVLQPSNTSRQKSLPLQKTNATAPSPPPVFQSSHDMLKKNNNAAHSATSENKFTDMA 845

Query: 61   --EKSKASDFFSVTRSDSVQKSNINLDQKSFIFTISSKQTPTLKDSIDTSNSDNQKTANA 120
              EKSKASDFFS TRSDSVQKS IN+DQKS IFTISSKQTP  +DSI TSN DNQKTAN 
Sbjct: 846  CPEKSKASDFFSATRSDSVQKSKINVDQKSSIFTISSKQTPPPEDSIGTSNVDNQKTANV 905

Query: 121  KERHTTTSPLFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLN 180
            KERHTTTS LFGSA+KPES  VGTM SLVPTVD ARK EEK+S+TTIS SV AP  A LN
Sbjct: 906  KERHTTTSQLFGSANKPESPFVGTMPSLVPTVDGARKTEEKKSVTTISQSVSAP--APLN 965

Query: 181  TTSSSSTLFSGFAVSKPLPSS---AAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 240
            T+SS+STLFSGFAVSK LPSS   AAV+DLNQP STSTQLNF SPVVS S+SLFQAPK V
Sbjct: 966  TSSSASTLFSGFAVSKSLPSSAAVAAVVDLNQPQSTSTQLNF-SPVVSGSNSLFQAPK-V 1025

Query: 241  STSPTLSSLNPSLEPLKKELPILKSDDDTEKQTPASKPESYELKFQPSVTPADKNHVEPT 300
             TSPTLSSLNP++E  K EL +LKS+DD EKQT +SKP S+ELKFQPS+TPADKNHVEPT
Sbjct: 1026 PTSPTLSSLNPTMESSKTELSVLKSNDDAEKQTLSSKPGSHELKFQPSITPADKNHVEPT 1085

Query: 301  SKTQTVAKDVGGQVPNVIGNAQPQQPSVAFA---SPNLTSKIFANGRNEASNTAVTQDDD 360
            SKTQTV KDVGGQVPNV+G+AQ QQPSVAFA   S NLTSKIFAN RNE SN  VTQDDD
Sbjct: 1086 SKTQTVFKDVGGQVPNVVGDAQAQQPSVAFASIPSQNLTSKIFANSRNETSNAVVTQDDD 1145

Query: 361  MDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMTSSFTMASPP 420
            MDEEAPETNNNVEFNLSSLGGFGNSSTP+SGAPKPNPFGGPFGNVNA S+T+SF MASPP
Sbjct: 1146 MDEEAPETNNNVEFNLSSLGGFGNSSTPISGAPKPNPFGGPFGNVNAASVTTSFNMASPP 1205

Query: 421  SGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMATQAPSQGGFGQPAQIGVGQQA 480
            SGELFRPASFSFQSPLASQAASQPTNSVAFSG FGSA+ATQAP QGGFGQPAQIGVGQQA
Sbjct: 1206 SGELFRPASFSFQSPLASQAASQPTNSVAFSGAFGSAVATQAPPQGGFGQPAQIGVGQQA 1265

Query: 481  LGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGSSVGSGGFAGVG 540
            LGNVLGSFGQSRQLGP+LPGTG+GSPGGFSGGFT+ KPVGV            GGFAGVG
Sbjct: 1266 LGNVLGSFGQSRQLGPTLPGTGSGSPGGFSGGFTNAKPVGV------------GGFAGVG 1325

Query: 541  SGGGGGFGGVGSGGGGFAGTVSTGGGFAGASSTTGGFAAAAGGGFVGAAGGGFAGAAGGG 600
            SGGGGGFGGV    GGFAG  STGGGFAGASSTT                GGFAGAAGGG
Sbjct: 1326 SGGGGGFGGV----GGFAGAASTGGGFAGASSTT----------------GGFAGAAGGG 1385

Query: 601  FAGAAGGFGAFGSQQGS-GGFSAFG---AGGAGGTGKPPELFTQIRK 633
            F G AGGFGAFGSQQ S GGFSAFG   AGGA  TGKPPELFTQIRK
Sbjct: 1386 FGGTAGGFGAFGSQQVSGGGFSAFGTAAAGGASVTGKPPELFTQIRK 1396

BLAST of Lsi04G007530 vs. TAIR 10
Match: AT1G55540.1 (Nuclear pore complex protein )

HSP 1 Score: 188.0 bits (476), Expect = 2.4e-47
Identity = 249/671 (37.11%), Postives = 333/671 (49.63%), Query Frame = 0

Query: 18   SLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFTEKSKASDFFSVTRSDSV--- 77
            S+Q+ + T  S  S F+SS     K     FS +S         +   F    S S+   
Sbjct: 1182 SVQRLSTT--SSGSDFESS-----KGFGAQFSTMSSGAPASSFSSKSLFGFNSSSSIPGD 1241

Query: 78   ---------QKSNINLDQKSFIFT-----ISSKQTPTLKDSIDTSNSDNQKTANAKERHT 137
                       S   LD  S +FT     +SS     +  SI  S++   +T +     T
Sbjct: 1242 KFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQDPVPASIPISSAPVPQTFSVTSTST 1301

Query: 138  TTSP----LFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNT 197
             ++      FG         +   +   P+           +L  +SPS P      +++
Sbjct: 1302 VSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGPTAGFTFNLPALSPSSP----EMVSS 1361

Query: 198  TSSSSTLFSGFA----VSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 257
            ++  S+LF   A    VS    S+ + +  +  L +ST L+ S+P ++  D+ FQ+P++ 
Sbjct: 1362 STGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTSLS-STPPITPPDA-FQSPQVS 1421

Query: 258  STSPTLSSLNPSLEPLKKEL---PILKSDDDTEKQTPASKPESYELKFQ-------PSVT 317
            + S  +    P  EP K E     IL +    +    A+K ++  L  +        +VT
Sbjct: 1422 TPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVT 1481

Query: 318  PADKNHVEP--TSKTQTVAKDVGGQVPNVIGNAQPQQPSVAFASPNLTSKIFANGRNEAS 377
            P   +      +S TQ+    +     +  G++QPQQ S   A    +S   A+   E  
Sbjct: 1482 PVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSSTPAPFPASSPTSASPFGEKK 1541

Query: 378  NTAVTQDDDMDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMT 437
            +   TQ+D+MDEEAPE +   E ++ S GGFG  STP  GAPK NPFGGPFG  NAT+ T
Sbjct: 1542 DIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPNPGAPKTNPFGGPFG--NATTTT 1601

Query: 438  SSFTMASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMAT--QAPSQGGFG 497
            S+    + PSGELF+PASF+FQ+P  SQ A           GFGS   T  Q P+Q GFG
Sbjct: 1602 SNPFNMTVPSGELFKPASFNFQNPQPSQPA-----------GFGSFSVTPSQTPAQSGFG 1661

Query: 498  QPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGS 557
            QP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP GF G             +G  +
Sbjct: 1662 QPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPTGFGGSNPG---------SGLPN 1721

Query: 558  SVGSGGFAGVGSGGGGGFGGVGSGGGGFAGTVST-GGGFAGASSTTGGFAAAA----GGG 617
            +  SGGFA  GS   GGF  + S G GFAG  ST  GGFA  +S +GGFA AA    GGG
Sbjct: 1722 APASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSGGFAGAAPGGGGGG 1781

Query: 618  F--VGAAGGGFAGAA----GGGFAGAA--GGFGAFG----SQQGSGGFSAFGAGGAGGTG 633
            F  +G+  GGF G A     GGFAGAA  GGFG FG     Q G GGFSAFG G +G TG
Sbjct: 1782 FGGLGSGTGGFGGFAPQGSSGGFAGAAGGGGFGGFGGQAQGQAGGGGFSAFG-GNSGATG 1816

BLAST of Lsi04G007530 vs. TAIR 10
Match: AT1G55540.2 (Nuclear pore complex protein )

HSP 1 Score: 188.0 bits (476), Expect = 2.4e-47
Identity = 249/671 (37.11%), Postives = 333/671 (49.63%), Query Frame = 0

Query: 18   SLQKANATVPSPLSVFQSSHEMLKKSNNEAFSVISENKFTEKSKASDFFSVTRSDSV--- 77
            S+Q+ + T  S  S F+SS     K     FS +S         +   F    S S+   
Sbjct: 1185 SVQRLSTT--SSGSDFESS-----KGFGAQFSTMSSGAPASSFSSKSLFGFNSSSSIPGD 1244

Query: 78   ---------QKSNINLDQKSFIFT-----ISSKQTPTLKDSIDTSNSDNQKTANAKERHT 137
                       S   LD  S +FT     +SS     +  SI  S++   +T +     T
Sbjct: 1245 KFTFPAVTAPLSGTPLDSTSTLFTASSAPVSSSSQDPVPASIPISSAPVPQTFSVTSTST 1304

Query: 138  TTSP----LFGSADKPESASVGTMSSLVPTVDEARKIEEKRSLTTISPSVPAPALARLNT 197
             ++      FG         +   +   P+           +L  +SPS P      +++
Sbjct: 1305 VSATGFNVPFGKPLTSVKVDLNQAAPSTPSPSPGPTAGFTFNLPALSPSSP----EMVSS 1364

Query: 198  TSSSSTLFSGFA----VSKPLPSSAAVIDLNQPLSTSTQLNFSSPVVSISDSLFQAPKIV 257
            ++  S+LF   A    VS    S+ + +  +  L +ST L+ S+P ++  D+ FQ+P++ 
Sbjct: 1365 STGQSSLFPPSAPTSQVSSDQASATSSLTDSSRLFSSTSLS-STPPITPPDA-FQSPQVS 1424

Query: 258  STSPTLSSLNPSLEPLKKEL---PILKSDDDTEKQTPASKPESYELKFQ-------PSVT 317
            + S  +    P  EP K E     IL +    +    A+K ++  L  +        +VT
Sbjct: 1425 TPSSAVPITEPVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVT 1484

Query: 318  PADKNHVEP--TSKTQTVAKDVGGQVPNVIGNAQPQQPSVAFASPNLTSKIFANGRNEAS 377
            P   +      +S TQ+    +     +  G++QPQQ S   A    +S   A+   E  
Sbjct: 1485 PVSSSGFLSGFSSGTQSSLASMAAPSFSWPGSSQPQQLSSTPAPFPASSPTSASPFGEKK 1544

Query: 378  NTAVTQDDDMDEEAPETNNNVEFNLSSLGGFGNSSTPMSGAPKPNPFGGPFGNVNATSMT 437
            +   TQ+D+MDEEAPE +   E ++ S GGFG  STP  GAPK NPFGGPFG  NAT+ T
Sbjct: 1545 DIVDTQEDEMDEEAPEASQTTELSMGSFGGFGLGSTPNPGAPKTNPFGGPFG--NATTTT 1604

Query: 438  SSFTMASPPSGELFRPASFSFQSPLASQAASQPTNSVAFSGGFGSAMAT--QAPSQGGFG 497
            S+    + PSGELF+PASF+FQ+P  SQ A           GFGS   T  Q P+Q GFG
Sbjct: 1605 SNPFNMTVPSGELFKPASFNFQNPQPSQPA-----------GFGSFSVTPSQTPAQSGFG 1664

Query: 498  QPAQIGVGQQALGNVLGSFGQSRQLGPSLPGTGTGSPGGFSGGFTSLKPVGVGGFAGAGS 557
            QP+QIG GQQALG+VLGSFGQSRQ+G  LPG   GSP GF G             +G  +
Sbjct: 1665 QPSQIGGGQQALGSVLGSFGQSRQIGAGLPGATFGSPTGFGGSNPG---------SGLPN 1724

Query: 558  SVGSGGFAGVGSGGGGGFGGVGSGGGGFAGTVST-GGGFAGASSTTGGFAAAA----GGG 617
            +  SGGFA  GS   GGF  + S G GFAG  ST  GGFA  +S +GGFA AA    GGG
Sbjct: 1725 APASGGFAAAGSSATGGFAAMASAGRGFAGASSTPTGGFAALASGSGGFAGAAPGGGGGG 1784

Query: 618  F--VGAAGGGFAGAA----GGGFAGAA--GGFGAFG----SQQGSGGFSAFGAGGAGGTG 633
            F  +G+  GGF G A     GGFAGAA  GGFG FG     Q G GGFSAFG G +G TG
Sbjct: 1785 FGGLGSGTGGFGGFAPQGSSGGFAGAAGGGGFGGFGGQAQGQAGGGGFSAFG-GNSGATG 1819