Lsi04G007180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G007180
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA pseudouridine synthase
Location	chr04: 6573760 .. 6581414 (-)
RNA-Seq Expression	Lsi04G007180
Synteny	Lsi04G007180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAAGCAAACTCCCATCGTCTACCTCGGGCAGTGACGAGGTAACTTCTTCAATTTATGGCAGAGTTCAATCAAACACTCGTCTTTCAACTCTACAATGCTTTAAACTAACACGGGGACACTTACACGGGGACACTTGCTACATTGCTTAAGCTTTGGATTCACTGTTCGATTCCCATAATCTTGGCAGGAGTGGCGAAAATCAATGACGGAAAACCATAAGCATGGACTAGAATTCAGTTTCGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTCGTCGCAGTCGAGTATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGTACTTTCCCTTTTTTTTTTTTTACACTGCCTTTCCTTCTTAACCAATGAAACTCTTTGGAACGGGACACAATTTCTTTGTATTGGCGTTCTGTTTAGCTGGTTTAAATGTTTTTGACAATTTGTGCCTTGAGGGACCAAAAGGAAGATGAACTTTTACAAGTTACATTGTCCTTTTTTCTTTTTTAGTAATCGTTGGTAATGAATATGAAACATCTAGACTGATATATCACATTAAGTAACATTTATGAAGGATCTTTACTGTTTGACACTTTTGTATCGTTTGCAAGTCATGATAGTATCAACTGTCATGGACTTCAGCTCAAGTTCTATTTCTATTTCTGCCCTTGTCGTCGTTTAAATGATTTTGAAAAGTAAGATTCATTCTTCAATGATGCATCTTCTTTTAAAGCATGTGCATAAGGGTAGGAGTAGTATGGTATTAGTAAGGGCATAAGTTAGGTAGTTTGAGGGTGGGGGATTGAAGGAAGACATGCAGTTGTTGAGTGATTCAAGGCTTGGGTTAGCATACTCAAGATGGGTGGTTCCAAGTACTTTATATTTGGTTTATCTTGTAGTTCTTCAGACTTTGTATTTCAATATATTTTAGATCTTGATTCCTGTTAGGTGGTACTCTAACGTATGTTTCTTTGAAAATGTCTACCTTACACTTGTCAGGAAGCATTCCAGAAGTTTATCGGTCAACCAGTCTCAGTGTTTTGTTCTAGCAGAACTGTAAGTTTATCTTATTCAGGATATTTGATCCTTTTTGTCATACAAAGCAATCTTGTTGCTGTAATTACTTATTAGAATGTTTACTATAATCGTAGGATATTATTAAACCAGATGAAGATATAACCTCATTTAAATTGAAAAGTAGTTTAAAGATAGTTCTCTCACAAGGTTCCACGACTTTTATCTTATTGATTCTTAAGTCCTCTGGTAGTCTTTCTCCCACTAGCTTTGATATGCACAGAGAAAGATATGCAATTGAACTGATAATTTGACTGTTCGCTTAACGATGTTGAAATTCTTGAACTTATGTTCATTTGAAATAATGGACTCGCCTTTATGTTCTAAGTCGGATTTTCAAACAAATCCATTGAGACATTTTAGGATAAGTTGGCTTTCTGGCTTGTTTGCTGCAGAGTGCAATGCATCTTGCTCCATATTTGTCTTTTCTGATGTGTGCAAATTGGAAGGGGATCTTTGATTACATTGATTTTTAGCAAAAGAAAATTAATATTTTTATCTACTCGAAATTTATGGATCAATCATATTCTTATAAATTACGTTTAGTTTATGGCAAATATGCGAATTAGTTTCAGGGTAAATTATTTTGAGATTTTCTGCTGAACTGCAACCTGCATCATATGCATGTACCAAGTATAGTTTTATTAAATTTATCAATTCAATTGTTTTTGCTTTCTTTGATATTCGAACTCCCATGTGTTATACTACGTTTCTTTCTAACATAATTATATTGGGACATCCTGTTCACGTTGTGACTTTCCTTCCTTTTTTCAATCAAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATTAGCAAAAGAAAGCCTGGAGAAGTGGTTTGTCCTTTTATAATAGAATATCAGCATCCGTATTTCCTCTCATATGTACACTTCTAATGTTTGCTCAACTAGATGAGTAATTTTCCCTGAACCTTTAGTTCACTAATTGTCTTTATTGTTGATTAAACTTTCTTATACATGAAAACATATGAGTTTGAAGAAATGGTGCTCAACTTTGCCGCAGGTATGCTAACTTATTTTTCCCGACTATGATGCAGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGGTTTGGTTCTCCACTGGCCAATCACTTTTTTACCATTACTATTGACTATTGTTCAAAGGCTATAGATGAGAGAGCACGTGAGAAGAACTTCCTTTTTCCAGCTTGAGCCTTTGTTTTTTTATTGCTTAACAAAGAAAATGATGAATGAAAGGTATTTCTGTTGATTTTGATTTCAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTCCATGCTAGATATAAAGCTCAAGAGCGTACGTGAGTATCATCCATCCATGCTTTAGTAGTTGATACTGTTGTTGCATATTATATAGTAGAACTAATTGTACATACCTTTATAAAAAACAAGTTTTGTGTTTCATGAATTATTTTCAGATACTTTTATCGGTTAGTTTCTGGGCCGGAAGCTCTGTCAAGCTTCGAGAAGGATCGAGCATGGCACGTGTCTGAGACACTAAATATTGGGGCAATGCAGGTATGAACAAATAACAGTGCAATGCTGGAGATCATATAGATTTTGAATTACATTTCAGAATTGATGCAACATTATTGGAATGTGTAGCGACCTTACTTGAATAAGATCTGTTCTATAGATTGCACAACTATCTCCATGGGTTCTAAAAGTTATTGGAATGTTTGAAATCAGGAGGCATGCAAGGTGCTTGTTGGGCATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGTCACTTAATACTAAAATTATTTATGTTCCCAGCTTATTATACATTTTGTTTGTAACAAAAGCCGGGGCGTGGTCGGGGAATGAAACGGTGTATGGTATAATGTAAATTAGTTTAGATGTGCATTGTGCAGTGCAGGCAAACTCACCAATCAGAACTCTAGATGAACTAGACGTTTGTGAGGTGGTTTCAACGCCTTATTTTCCAACATCAATGGAGCGAAATCAAAGGCAGGGCAAAGAAGAAGGTGAGAATGAAGTGTCACATTCAGAAACTTGTGAGGTTGGATTTGGAATCAGGAGAAGACATCGTTGTTTTGTGGTGACAGCACGAGCACGCTCTTTTCTCTATCATCAGGTTCAAAATCACCTACACTAGAAGACTTTGTGCATATTTGGAAATGATTTTGAAATTGTTCAAATCGTTTTTGTTTGTTCAAAATCACTCCAAAACACGTCTTAAATCACTTGAAATAAATCTAGGCTCCATTTGGTGACTACTTTGTTTTTAGTTTTTAGTTTTTGAAAATTAAGTCTATAAGCATTCCTTTCACCCCCTAAATTTTTTGCGTTGTTATCTACTATTTATCAGTATTTTACAAAACTAAAACAAGTTTTGAAAACTAAAAAAATTACGTAAAAACTTTCAGAATTTGGCAAAGAATTCAACTCTTTTTTACTTGAGAAATATTTCAACTATTGTAAGAAACCGAAAGGATATATGCCTAACTTTCAGAAATCAAAAACAAAAATCCTTATGGTTACCAAAAGAGGGCCCTTAATATTTAATTTTACGCGTTTAAATACAATTTTCACATCATCAAAATTACTTTGGAATCATTGAAAGCATTTTTCATAGTGATTTTAACTGTCATACATGCCCTTCTTTTCTTGTTCATGAGATCGATTAATTAGTTATTCTGGAATGGACTATATTTCTCAGGTGAGACTGATGGTTGGAGTTCTTAAAGCGGTCGGCACCGGAGATCTAACAGTAGGAGATGGTAGGTGGCTGAGCAGCAACATATACAACAGCAATATTTGTAGTTTTTTTCTTTGAATTAATAGATCATCGCTGTTTCAAGGTAGACTGGACTTGCCAGTGGCCTAACAATAATATGGTCTGTGTCTTATTTGCTGCAGTAGAAAAAATCTTGGAAGCGAAGAATGTGAGTTCAGCCAGTCCAATGGCTCCTGCATGCGGACTCTATCTCGGACATGTCAAATATGATCTACCGCCAGCTCCCCACTGAATGAAACCTCCATTTCTTTCTTCACTTCCGAGGATTAAAATACGGGTATATGACGAATATTTTTTTTTTTAAGTCACGAATTTTATAAAATTTATTTAAATTAATAATTAAGTTATTTTTAATGTTTTACAACCATTTATAAAAGATGTTAGAGATATTTATAGATATTTTTAATATTGTTGTCGAACCCTTTTAGTTTATAGTTTATAGATGTATCGATGGATTTATTGATATGTTGACGAATATTTTTATTGTTGTTCAGTTCAAATTTTATTTATTTCAATTGATTGCGAATATGGTTGGCAACGGGGCGAGACGGGGAGAATTCCTAACCCCATCCCCACCAATTGAAGATAGAGATGGGGATGGGGATGGGGATTTCCCAATCGGGGAATGAGTCCATGTGGGGACTAATCCTCGAGTTAAATACAAAAAAGAATAAAGAATAATAAATACAAAAATTAAAAAGAAAATAAAAACAGTAAACATAGAATATATATATATATATAAAGCGGGGTGGGGATATCCTCCCAATTAAAATCTCCTGTCAAACAAGGGATTCTTCGATCAGGTCGGGACCTGCCGGATTTTTTTTCTTTTGTTGACATTCAAAATCAATGGTTGAATTGAAGTTGAAACCCATTCCACTTTGTACGGATTTATTCTCTCCTATTTATGATCACTCAAATCTGTCCATTTTAAAAAATAAAAGATACATAACAATGAAAATATGTTCCGTACACTTATTTTCAAAAACATTTTTTTCGAAAACAATATTATGCCGTTTCCATTGTTCTTTTAGTTTTGTTTTCAAAATTTAGAAAACCTGAGAAACAACCTTTAAAAAAAAAAAAGCCAAAAGACGTCTGTGTCCAATAAATCACACTTCATTGTTAATTATTTCAACTTTTTTTATTCTTAAAAAATAGATTTTACCAAACACATATTAATCTTTTTCCACATTTTTAAATTCACAGCCCAACACAATTTTCAAAATTTCTATTCAAATTTAAAGTCCTTAAATTGGAACACCAAATGCATTTTTAAATACAGTGAAACAAGGAATTTGTTTTCATTTCCTGCAAAAATTCTCAAATTTGTAAACGTTTCCAAGGCCATTCGAAGGCGGAAAAATGATTTTCAATATGGAGCCAATTACAATACAACTAACTACACCTAGTCATATATAATTTTATGATACATGCATGAAACACGAAAAAGCCTATCAGTATTTAGGTAATACAATAATTTAGCTACTACTTCATGAACTCTTCTATGGCCATGTGAATAGAGCTAATTCGAGAAGCACGGTGCTGTGGAAGCAATGAATGGGGTCCTCTCCACACGGATCTTGAATTCACAGGGAAAACGACTCCACACAACTCTAAGACCATCGGGTTTTTAGTGCTCGACTCCTCCCATGCCTTTAGTTTCTTCCTTCGTTTTTCTTCTACCTCCTGTCATCAAACCATTTGTTAAATCATCGATTGATCCAAAAGTTTAATATTATATCATCTAACACTCTCCCTCACTTTTGGGCTTGAAATATGTAGAAGATCCAACAAGTGGTAGTCAATATTAATTGGGGAGAAAATAACATTGCAGGGATTTAAACATGGGACCTCTTGAACCACCTGTTCTAATACCATGTTAAATCATCGATTGACCTAGAAGCGTAAGCTAATGGACGAAACTAAATTTAATATTATATCATCTAACACCATTAACATGCTCAATCTGTTTTTCCTCTACTTCCTTGGTCCCCTCTTTCTTTTCGTCTTTTGGTTCTTGTTTAATGGAAGCTATGTTTGCTATCAATTAAATAAGATTAACCAATTTCTGAAATTAATTTAACCAGTTTTTCGTTCCTAGTTCAATGATGGTTTGTTTAATGGTCATTATTTCAACAAAAAGAAAGCAAAATACCTTATGTTCCAAGTTATGAATATCTTGTTGTCCACCCTCATGCTGTACATCCTCCATTTTCAAGTCACCTGATTCCTCCATTGTTGATTTCAATGGTCTATTGTGATCCTCCCTTGACCTTTCCGACTGCTCGGTTCCTTTAGAATCGGCGCCTGTCCTCTTCCCCATTTCAGTTTCTTCATCTTTTTCCTCCACACCTTCATCTTGCTTATCATCTGGGGTGCTAATCTGTTGCTTTGAAACATCCATTTTCACCTCGTCTCCTGTTTCTTCCTCAACACCAAGTTCATTGTCTAGTGAATCATCACCATTTGATGATGAATCCACGCTGCCAGGACTTTCCCCACGCAAAGATTCCTTAACAAAAAGGAAAAAAAAAAATACAGATGGAGCATTATTCTTTTTATATCCTATAATAAAGGAAAACAAATGGACAGAAAATAGACAGAAAGTAATCAATAAGCAAGAGACGTCAATCAACATTTCTAGGATGAAAGAAATGCTAAAATCTTCCAAGATAACGTCAAGTTGACAAAGGACATTATAGATACTATCATTCTCTTTTTTTGGTGCAAAGATATACCCGCTATACATATTCATAGTTTTTCTTTTCTTATTGCAAATTAGAAACAACTTTTGTATATCCTTTGATTGGATCCCTTTTGTACCTTGGACATCTTTGGATATTTCATTCCATCAATAAAATTGTTTTTTATAAGTTTAAAAGAAAAAAAATCATTTCTGGCGGCTGAAGGAGTCATAATCTAATCTCAATATCAATATTTTCCCCAAATCAAGAAAAGTGAATTAAATTCGCAGAAGTCACCTCAAACTTGAACTCCTGCGCTACGTTGTTCCCAGCTCCATTTTGGTCTTTACCAATTCTAGCAGTCTCTTCACGCTCCTTCCTACGGCGCTTTAAAACACATATTGCACATAAACAATCAGATTTATGGCGCCTTCTGCAAAAAAGAAACACTTCAATCAGCATATCACTTCAGAAGTTCTATGCTCAAGAAACGTATAAGAAATAAAGAGACTTTATTCCATACAAACATCAATGTTAGTAACTTCACGACAGATATGGGGGTAAAGTCTAAAAAGTAAACTTCCAACTAGCTAGCCAGAGAATTTACTGCCAAGTTAAAAAAATTTCATTCAACTAAACTCATTTGGTTTTTTTGTAGCTGTACTGGCAATTTCCCTCTAGTATCACGTTTTGAGAATGTCCTATATCCAGACTGTTTCATTGCAACAAAGTAACTCCAGCACTTCCCTCATCAACTAACCATGAACATAAGGTTAAATTTTTGTTCTACCGTGCAACTCAAGAGCCATAAATGCAAAATGCATCTGATGTAACTTTATGCAGGTTGCAGAATCATACCCATGTCGCTTCTTCGATTTCTGTTTTGACTGACCTTTCAGAGCTTTCACCTGACTGGAT

mRNA sequence

GCAAAGCAAACTCCCATCGTCTACCTCGGGCAGTGACGAGGAGTGGCGAAAATCAATGACGGAAAACCATAAGCATGGACTAGAATTCAGTTTCGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTCGTCGCAGTCGAGTATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATTAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTCCATGCTAGATATAAAGCTCAAGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCGGAAGCTCTGTCAAGCTTCGAGAAGGATCGAGCATGGCACGTGTCTGAGACACTAAATATTGGGGCAATGCAGGCAAACTCACCAATCAGAACTCTAGATGAACTAGACGTTTGTGAGGTGGTTTCAACGCCTTATTTTCCAACATCAATGGAGCGAAATCAAAGGCAGGGCAAAGAAGAAGGTGAGAATGAAGTGTCACATTCAGAAACTTGTGAGGTGAGACTGATGGTTGGAGTTCTTAAAGCGGTCGGCACCGGAGATCTAACAGTAGGAGATGGTAGTAGAAAAAATCTTGGAAGCGAAGAATGTGAGTTCAGCCAGTCCAATGGCTCCTGCATGCGGACTCTATCTCGGACATGTCAAATATGATCTACCGCCAGCTCCCCACTGAATGAAACCTCCATTTCTTTCTTCACTTCCGAGGATTAAAATACGGCTGTACTGGCAATTTCCCTCTAGTATCACGTTTTGAGAATGTCCTATATCCAGACTGTTTCATTGCAACAAAGTAACTCCAGCACTTCCCTCATCAACTAACCATGAACATAAGGTTGCAGAATCATACCCATGTCGCTTCTTCGATTTCTGTTTTGACTGACCTTTCAGAGCTTTCACCTGACTGGAT

Coding sequence (CDS)

ATGACGGAAAACCATAAGCATGGACTAGAATTCAGTTTCGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTCGTCGCAGTCGAGTATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATTAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTCCATGCTAGATATAAAGCTCAAGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCGGAAGCTCTGTCAAGCTTCGAGAAGGATCGAGCATGGCACGTGTCTGAGACACTAAATATTGGGGCAATGCAGGCAAACTCACCAATCAGAACTCTAGATGAACTAGACGTTTGTGAGGTGGTTTCAACGCCTTATTTTCCAACATCAATGGAGCGAAATCAAAGGCAGGGCAAAGAAGAAGGTGAGAATGAAGTGTCACATTCAGAAACTTGTGAGGTGAGACTGATGGTTGGAGTTCTTAAAGCGGTCGGCACCGGAGATCTAACAGTAGGAGATGGTAGTAGAAAAAATCTTGGAAGCGAAGAATGTGAGTTCAGCCAGTCCAATGGCTCCTGCATGCGGACTCTATCTCGGACATGTCAAATATGA

Protein sequence

MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLEDAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGAMQANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLKAVGTGDLTVGDGSRKNLGSEECEFSQSNGSCMRTLSRTCQI

Homology

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BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot
Match: Q8N0Z8 (tRNA pseudouridine synthase-like 1 OS=Homo sapiens OX=9606 GN=PUSL1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.3e-17
Identity = 86/280 (30.71%), Postives = 124/280 (44.29%), Query Frame = 0


Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHAL 108
           RYLV  +Y+GT F+G       +  VGV   LE                    DAGVHAL
Sbjct: 13  RYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFTISSRTDAGVHAL 72

Query: 109 SNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYK 168
           SN  H+DV+R S R      PP  P V+  A+N  L +H   + V+    VP+DFHAR+ 
Sbjct: 73  SNAAHLDVQRRSGR------PPFPPEVLAEALNTHL-RHPA-IRVLRAFRVPSDFHARHA 132

Query: 169 AQERTYFYRLVSG---PEALSSFEKDRAWHV-SETLNIGAMQ------------------ 228
           A  RTY YRL +G    + L  FE++  W + ++ L++ AMQ                  
Sbjct: 133 ATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQSA 192

Query: 229 ---ANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLK 276
                SP+RTL  + V    ++P       R  R    E E++       +VR M  VL 
Sbjct: 193 GSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYR--QVRRMTAVLV 252

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BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot
Match: Q6GQ53 (tRNA pseudouridine synthase-like 1 OS=Xenopus laevis OX=8355 GN=pusl1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-15
Identity = 72/252 (28.57%), Postives = 113/252 (44.84%), Query Frame = 0


Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHAL 108
           RYLV  +Y GT++SG  + P  ++V+GV   LE                    D+GVHA+
Sbjct: 7   RYLVFFQYYGTKYSGVMETPITQSVLGVQNYLEVAAQKLRPVGTIKFFISSRTDSGVHAI 66

Query: 109 SNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYK 168
            N+ HVD+ER   +      PP    ++ +A+NH LQ     + ++    V ++FHAR+ 
Sbjct: 67  CNLAHVDIERAKGK------PPFTEEILVQAMNHHLQPE--PIRLLKAIRVCDNFHARHN 126

Query: 169 AQERTYFYRLVSG---PEALSSFEKDRAWHVSETLNIGAMQA------------------ 228
           A  RTY YR+ +G   PE L  FE +  W + + LN+ A+                    
Sbjct: 127 ALSRTYVYRVAAGCSRPE-LPVFEGNLCWAIQDGLNVTAINEASKMLLGTHNFSAFRSAN 186

Query: 229 -----NSPIRTLDELDV---CEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMV 249
                 SPI+TL + D+   C +++  +   +++        +           +VR M 
Sbjct: 187 SENAFKSPIKTLQQADINPSCGILTHHWQSRNLQ------FWDFTFRARSFLYKQVRRMT 243

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BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot
Match: Q9ZCA3 (tRNA pseudouridine synthase A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=truA PE=3 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-15
Identity = 63/211 (29.86%), Postives = 97/211 (45.97%), Query Frame = 0


Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVC 108
           RY + +EY+GT F+G Q+Q    +V  +LE                   DAGVHA+  V 
Sbjct: 3   RYKITIEYLGTHFAGWQRQAGVLSVQQILEEAIYKFSSEQVTLFGSGRTDAGVHAIGQVA 62

Query: 109 HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           H D+ +             EP  + +A+N+F++ +  DV V +   V N+FHAR+ A  R
Sbjct: 63  HFDLSKYL-----------EPYKIIKAINYFVRPY--DVGVWNCELVSNNFHARFSAISR 122

Query: 169 TYFYRLVSGPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPI 213
            Y YR+++     S  + +RAW +S  L+I AM                     Q+ SPI
Sbjct: 123 HYIYRIINRTYP-SVIDFNRAWWISSPLDILAMQKAAAYLLGKHDFTSFRSSSCQSKSPI 182

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BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot
Match: Q6G547 (tRNA pseudouridine synthase A OS=Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) OX=283166 GN=truA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 8.9e-15
Identity = 70/250 (28.00%), Postives = 107/250 (42.80%), Query Frame = 0


Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVC 108
           R+ + +EY G+ ++G Q+Q   RT+   LE                   DAGVHA   V 
Sbjct: 3   RFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQVA 62

Query: 109 HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           HVD E+  +              V  A+N  LQK   ++ ++ V++VP+ F AR+ A +R
Sbjct: 63  HVDFEKNWRTH-----------TVRDALNAHLQKQGDNIAILHVQNVPDSFDARFSAIKR 122

Query: 169 TYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QANS 228
            Y +++++   P AL++    R W + + LN  AM                     QA S
Sbjct: 123 HYLFKILNRRSPPALNT---KRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFRSAHCQAKS 182

Query: 229 PIRTLDELDVCEVVSTPYFPTSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVLKA 252
           PIRTL+ LDV                QR+G+E     +  + + H    ++R   G L  
Sbjct: 183 PIRTLERLDV----------------QREGEEIFLYAQARSFLHH----QIRSFAGSLME 218

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BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot
Match: A9ILJ7 (tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) OX=382640 GN=truA PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 69/252 (27.38%), Postives = 110/252 (43.65%), Query Frame = 0


Query: 47  IQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSN 106
           + R+ + +EY G+ ++G Q+Q    T+ G LE                   DAGVHA   
Sbjct: 1   MSRFKLTLEYDGSNYAGWQRQAKLHTIQGALEQAIFRFSGQQLTITTAGRTDAGVHATGQ 60

Query: 107 VCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQ 166
           V HVD  +          PPH    V  A+N  L++    +++++V +VP+DF AR+ A 
Sbjct: 61  VAHVDFIK--------NWPPH---TVRDALNALLRQQSEAISILNVENVPDDFDARFSAI 120

Query: 167 ERTYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QA 226
           +R Y +++++   P AL++    R W + + LN  AM                     QA
Sbjct: 121 KRHYLFKILNRRSPPALNA---KRVWWIPKPLNAQAMHEAAQKLVGQHDFTTFRSAHCQA 180

Query: 227 NSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVL 252
            SPIRTL+ LD+                Q++G+E     +  + + H    ++R   G L
Sbjct: 181 KSPIRTLERLDI----------------QKEGEEIFIYAKARSFLHH----QIRSFAGSL 218

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BLAST of Lsi04G007180 vs. ExPASy TrEMBL
Match: A0A5D3CZZ6 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002730 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-118
Identity = 235/328 (71.65%), Postives = 240/328 (73.17%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. ExPASy TrEMBL
Match: A0A1S3BDG9 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-118
Identity = 235/328 (71.65%), Postives = 240/328 (73.17%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. ExPASy TrEMBL
Match: A0A1S3BDY4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.6e-118
Identity = 231/316 (73.10%), Postives = 235/316 (74.37%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 252
           STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. ExPASy TrEMBL
Match: A0A0A0KRX0 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.2e-116
Identity = 232/328 (70.73%), Postives = 238/328 (72.56%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTDE+  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           F GSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGKEE E EV  SETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. ExPASy TrEMBL
Match: A0A6J1KFZ8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.5e-113
Identity = 227/328 (69.21%), Postives = 239/328 (72.87%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KH LEFS DSS QRPLKIGKTT+E L GD+EEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHRLEFSSDSSPQRPLKIGKTTNEDLPGDKEEAESMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVGVLE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGVLEEAFRKFIGQSVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVV RAVNHFLQK++GDVTVVDVRSVPND+HARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVRRAVNHFLQKNDGDVTVVDVRSVPNDYHARYKAQERTYFYRLVSGPET 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSF+KDR WHV E LNIGAM                     QANSP+RTLDELDVCEV+
Sbjct: 181 LSSFDKDRVWHVPEILNIGAMQEACKVLVGHHDFSSFRAAGCQANSPMRTLDELDVCEVI 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFP+SMER QRQGKEEGENEV HSE+ E                         VRLM
Sbjct: 241 STPYFPSSMERKQRQGKEEGENEVLHSESYEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. NCBI nr
Match: XP_038877867.1 (tRNA pseudouridine synthase A isoform X2 [Benincasa hispida])

HSP 1 Score: 439.9 bits (1130), Expect = 1.7e-119
Identity = 240/328 (73.17%), Postives = 244/328 (74.39%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLEFS DSS QRPLKIGKTTDEVL   EEEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHVSETLNIGAM                     QANSPIRTLDELDVCEVV
Sbjct: 181 LSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGK EG++EV HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGK-EGDSEVPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. NCBI nr
Match: XP_008445970.1 (PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa] >TYK15769.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 435.6 bits (1119), Expect = 3.1e-118
Identity = 235/328 (71.65%), Postives = 240/328 (73.17%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. NCBI nr
Match: XP_038877866.1 (tRNA pseudouridine synthase A isoform X1 [Benincasa hispida])

HSP 1 Score: 435.3 bits (1118), Expect = 4.1e-118
Identity = 240/340 (70.59%), Postives = 244/340 (71.76%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLEFS DSS QRPLKIGKTTDEVL   EEEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------------------DAGVHALSNVC 120
           FSGSQ+QP CRTVVG LE                               DAGVHALSNVC
Sbjct: 61  FSGSQQQPNCRTVVGALEVAEDDVLKMSLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 120

Query: 121 HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 180
           HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER
Sbjct: 121 HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 180

Query: 181 TYFYRLVSGPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPI 240
           TYFYRLVSGPEALSSFEKDRAWHVSETLNIGAM                     QANSPI
Sbjct: 181 TYFYRLVSGPEALSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSPI 240

Query: 241 RTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE----------------- 260
           RTLDELDVCEVVSTPYFPTSMER QRQGK EG++EV HSETCE                 
Sbjct: 241 RTLDELDVCEVVSTPYFPTSMERKQRQGK-EGDSEVPHSETCEVGFGIRRRHRCFVVTAR 300

[Close]

BLAST of Lsi04G007180 vs. NCBI nr
Match: XP_008445972.1 (PREDICTED: tRNA pseudouridine synthase A isoform X2 [Cucumis melo])

HSP 1 Score: 434.9 bits (1117), Expect = 5.4e-118
Identity = 231/316 (73.10%), Postives = 235/316 (74.37%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           FSGSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 252
           STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. NCBI nr
Match: XP_011655540.1 (uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hypothetical protein Csa_008571 [Cucumis sativus])

HSP 1 Score: 426.4 bits (1095), Expect = 1.9e-115
Identity = 232/328 (70.73%), Postives = 238/328 (72.56%), Query Frame = 0


Query: 1   MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTEN KHGLE S DSSSQRPLKIGKTTDE+  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKP 120
           F GSQ+QP CRTVVG LE                   DAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVV 240
           LSSFEKDRAWHV ETLNIGAM                     QANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM 260
           STPYFPTSMER QRQGKEE E EV  SETCE                         VRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

[Close]

BLAST of Lsi04G007180 vs. TAIR 10
Match: AT1G09800.1 (Pseudouridine synthase family protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.2e-69
Identity = 164/343 (47.81%), Postives = 196/343 (57.14%), Query Frame = 0


Query: 1   MTENHKHGLE-FSFDSSSQRPLKIGKTTDEVLRGDEE-----EAEPMNR-NPGIQRYLVA 60
           M++N KH L+  + + S +R  K+ +         EE     + E MN  NP +QRYLVA
Sbjct: 1   MSDNLKHPLDCTASEESLERLSKVARIEGFSDEESEEQQGLADLESMNPVNPRVQRYLVA 60

Query: 61  VEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVE 120
           +EYIGTRFSGSQ+Q   RTVVGVL+                   DAGVHALSNVCHVDVE
Sbjct: 61  IEYIGTRFSGSQQQAKDRTVVGVLQEAFHKFIGQPVKIFCSSRTDAGVHALSNVCHVDVE 120

Query: 121 RISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYR 180
           RISKRKPGEVLPPHEPGVV RAVNHFLQ+ +GDV V+DVR VP+++HARYKAQERTYFYR
Sbjct: 121 RISKRKPGEVLPPHEPGVVQRAVNHFLQRKDGDVMVIDVRCVPSNYHARYKAQERTYFYR 180

Query: 181 LVSGPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDE 240
           L+SG + LS  EKDR WHV E LN+  M                     QA SP+R+LDE
Sbjct: 181 LLSGSDPLSILEKDRCWHVPEELNLRFMQEACRVLVGSHDFSSFRAAGCQAKSPMRSLDE 240

Query: 241 LDVCEVVSTPYFPTSMER--------------NQRQ----------GKEEGENEVS---- 255
           L++ EV STPYFP+  ER              NQ +          G+ EG  E      
Sbjct: 241 LNITEVPSTPYFPSITERTLSNLNNGDPLACSNQPKSETAGVTTNVGEVEGSTEGDSFGR 300

The following BLAST results are available for this feature:

BLAST of Lsi04G007180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8N0Z8	4.3e-17	30.71	tRNA pseudouridine synthase-like 1 OS=Homo sapiens OX=9606 GN=PUSL1 PE=1 SV=1	[more]
Q6GQ53	1.8e-15	28.57	tRNA pseudouridine synthase-like 1 OS=Xenopus laevis OX=8355 GN=pusl1 PE=2 SV=1	[more]
Q9ZCA3	1.8e-15	29.86	tRNA pseudouridine synthase A OS=Rickettsia prowazekii (strain Madrid E) OX=2729...	[more]
Q6G547	8.9e-15	28.00	tRNA pseudouridine synthase A OS=Bartonella henselae (strain ATCC 49882 / DSM 28...	[more]
A9ILJ7	1.2e-14	27.38	tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS ...	[more]

BLAST of Lsi04G007180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CZZ6	1.5e-118	71.65	tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BDG9	1.5e-118	71.65	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1	[more]
A0A1S3BDY4	2.6e-118	73.10	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1	[more]
A0A0A0KRX0	9.2e-116	70.73	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1	[more]
A0A6J1KFZ8	2.5e-113	69.21	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=...	[more]

BLAST of Lsi04G007180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877867.1	1.7e-119	73.17	tRNA pseudouridine synthase A isoform X2 [Benincasa hispida]	[more]
XP_008445970.1	3.1e-118	71.65	PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1...	[more]
XP_038877866.1	4.1e-118	70.59	tRNA pseudouridine synthase A isoform X1 [Benincasa hispida]	[more]
XP_008445972.1	5.4e-118	73.10	PREDICTED: tRNA pseudouridine synthase A isoform X2 [Cucumis melo]	[more]
XP_011655540.1	1.9e-115	70.73	uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hy...	[more]

BLAST of Lsi04G007180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09800.1	2.2e-69	47.81	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 45..79 e-value: 1.7E-5 score: 27.0 coord: 80..146 e-value: 1.1E-8 score: 37.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 29..80 coord: 79..250
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 29..80 coord: 79..250
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 47..250

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007180.1	Lsi04G007180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity