Lsi04G007020 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G007020 vs. ExPASy Swiss-Prot
Match: Q5H8A6 (Ion channel CASTOR OS=Lotus japonicus OX=34305 GN=CASTOR PE=1 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 629/982 (64.05%), Postives = 701/982 (71.38%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFP  SF  S P++   Y RRF   S              T S+ S ++         
Sbjct: 16  DWFFPSPSFFRSSPSQ---YGRRFHTNS-------------NTHSAPSSTYPSG------ 75

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
              R RRR  F      S              E P +S  + K S  S+ +    S   +
Sbjct: 76  --IRHRRRVKFSRTPTTSSN------------EKPQISIVSDKPSAISKNNLNWLSQFGL 135

Query: 121 RWHFLAIAV-------------STRISKLN---IKLRACNLFD--SGSEDDVRSADELAD 180
           ++  + + +              ++++KL    ++L AC+  D  + S      + +   
Sbjct: 136 QFALVTLTIVFLLLLLLRNTHLESQVNKLQGEILRLHACHQLDTLNVSSSTAHKSQDTHP 195

Query: 181 VVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYKVDVFFS 240
              +   + LAL  S  LLL P+II KYIDY+S+SR  ++  E+VSLNKQ+AY+VDVF S
Sbjct: 196 CSCENFKRNLALFLSFMLLLIPLIIFKYIDYVSRSRLSENISEQVSLNKQIAYRVDVFLS 255

Query: 241 IHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSEGIGPRL 300
           ++PYAKPL LL+ATLLLI LGGL LFGVT + L  CLWLSWTYVAD+GNHA+SEGIGPRL
Sbjct: 256 VYPYAKPLVLLVATLLLIFLGGLTLFGVTTEDLGHCLWLSWTYVADSGNHASSEGIGPRL 315

Query: 301 VSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLNQI 360
           V+VS+SFGGMLIFAMMLGLVSD+ISEKFDSLRKG+SEVVEQNHTLILGWSDKLGSLLNQ+
Sbjct: 316 VAVSISFGGMLIFAMMLGLVSDAISEKFDSLRKGKSEVVEQNHTLILGWSDKLGSLLNQL 375

Query: 361 SIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHTMSF 420
           +IANESLGGGT+ VMAERDKE+MELDI KMEFDFKGTSVICRSGSPLILADLKK      
Sbjct: 376 AIANESLGGGTIAVMAERDKEDMELDIGKMEFDFKGTSVICRSGSPLILADLKK------ 435

Query: 421 WSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLSLTG 480
                                     VSVSKAR IIV+AEDGNADQSDARALRTVLSLTG
Sbjct: 436 --------------------------VSVSKARTIIVLAEDGNADQSDARALRTVLSLTG 495

Query: 481 VKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDIL 540
           VKEGL GHIVVE+SDLDNEVLVKLVGG+LVETVVAHDVIGRLMIQCARQPGLAQIWEDIL
Sbjct: 496 VKEGLRGHIVVEMSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDIL 555

Query: 541 GFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEV 600
           GFENCEFYIKRWPQL GM FEDVLISFP AIPCGIKVAS GGKI+LNP+DSYVLQEGDEV
Sbjct: 556 GFENCEFYIKRWPQLDGMLFEDVLISFPAAIPCGIKVASYGGKIILNPDDSYVLQEGDEV 615

Query: 601 LVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKNF 660
           LVIAEDDDTYAPA LP VR                                 RGSLPK+F
Sbjct: 616 LVIAEDDDTYAPAPLPMVR---------------------------------RGSLPKDF 675

Query: 661 IVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPENEREKK 720
           + PKS ERIL CGWRRDMEDMI            VLDA LAP SELWMFNDVPE EREKK
Sbjct: 676 VYPKSPERILFCGWRRDMEDMI-----------TVLDASLAPDSELWMFNDVPEKEREKK 735

Query: 721 LVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSL 780
           L+DGGLDISRLENISLV+REGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSL
Sbjct: 736 LIDGGLDISRLENISLVNREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSL 795

Query: 781 ATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLS 840
           ATLLLIRDIQA+R+P     +   GG+FS+GSWIGEM+QASDK+VIISEILDPRTKNLLS
Sbjct: 796 ATLLLIRDIQARRLPYVAMASQTQGGNFSKGSWIGEMKQASDKTVIISEILDPRTKNLLS 853

Query: 841 MSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMRLLLWHT 900
           MSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE                      
Sbjct: 856 MSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---------------------- 853

Query: 901 HNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAERAVINP 960
                     GNE+HIRQAD+YLREGEE+SFYE++LRARQRREI+IGYR ANAERAVINP
Sbjct: 916 ----------GNEMHIRQADIYLREGEEMSFYEIMLRARQRREILIGYRLANAERAVINP 853

Query: 961 PAKNERRKWSVGDVFVVIAEKE 965
           PAK  RRKWS+ DVFVVI EKE
Sbjct: 976 PAKTGRRKWSLKDVFVVITEKE 853

BLAST of Lsi04G007020 vs. ExPASy Swiss-Prot
Match: Q75LD5 (Probable ion channel CASTOR OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0843600 PE=2 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 583/837 (69.65%), Postives = 648/837 (77.42%), Query Frame = 0

Query: 129 VSTRISKLNIKLRACNLFDSGSEDDVRSADELADVVTDKRLKALALIASLTLLLAPIIIL 188
           +  ++++   KL++C    S     + S         ++ LK  +L+ SL+ L AP++IL
Sbjct: 165 LKAQLAEATTKLQSCITESSMDMSSILSYQSNNSTSQNRGLKNFSLLLSLSTLYAPLLIL 224

Query: 189 KYIDYISKSRSLDHNLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALF 248
           KY+D   K RS   + EEV +NK+LAY+VD+F S+ PYAKPL LL+ATLLLI LGGLAL+
Sbjct: 225 KYMDLFLKLRSSQDSEEEVPINKRLAYRVDIFLSLQPYAKPLVLLVATLLLIGLGGLALY 284

Query: 249 GVTDDSLVDCLWLSWTYVADAGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE 308
           GV DDSL+DCLWLSWT+VAD+GNHAN+EG GP+LVSVS+S GGML+FAMMLGLV+DSISE
Sbjct: 285 GVNDDSLLDCLWLSWTFVADSGNHANAEGFGPKLVSVSISIGGMLVFAMMLGLVTDSISE 344

Query: 309 KFDSLRKGRSEVVEQNHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELD 368
           KFDSLRKGRSEV+EQ+HTL+LGWSDKLGSLLNQI+IANESLGGGT+VVMAE+DKEEME D
Sbjct: 345 KFDSLRKGRSEVIEQSHTLVLGWSDKLGSLLNQIAIANESLGGGTIVVMAEKDKEEMEAD 404

Query: 369 IAKMEFDFKGTSVICRSGSPLILADLKKTHTMSFWSMNTINFQLRISLFSLEVLLTSYIQ 428
           IAKMEFD KGT++ICRSGSPLILADLKK                                
Sbjct: 405 IAKMEFDLKGTAIICRSGSPLILADLKK-------------------------------- 464

Query: 429 VSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLGGHIVVELSDLDNEVLVKLVG 488
           VSVSKARAI+V+AE+GNADQSDARALRTVLSLTGVKEGL GHIVVELSDLDNEVLVKLVG
Sbjct: 465 VSVSKARAIVVLAEEGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVG 524

Query: 489 GELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLVGMQFEDVLIS 548
           G+LVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQL GMQFEDVLIS
Sbjct: 525 GDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLIS 584

Query: 549 FPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFIHI 608
           FPDAIPCGIKVAS GGKI+LNP+D YVLQEGDEVLVIAEDDDTYAPA LP          
Sbjct: 585 FPDAIPCGIKVASYGGKIILNPDDFYVLQEGDEVLVIAEDDDTYAPAPLPK--------- 644

Query: 609 ARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVML 668
                                   V RG LPK+F+VPKS ERIL CGWRRDMEDMIM   
Sbjct: 645 ------------------------VMRGYLPKDFVVPKSPERILFCGWRRDMEDMIM--- 704

Query: 669 LRSENMLPVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIR 728
                   VLDAFLAPGSELWMFNDVPE +RE+KL+DGGLD SRLENI+LV REGNAVIR
Sbjct: 705 --------VLDAFLAPGSELWMFNDVPEMDRERKLIDGGLDFSRLENITLVHREGNAVIR 764

Query: 729 RHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTT-AHG 788
           RHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKR+P R+A  +    
Sbjct: 765 RHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRLPFREAMVSHVTR 824

Query: 789 GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDR 848
           GSF +GSWIGEMQQASDKSVIISEILDPRTKNLLS+SKISDYVLSNELVSMALAMVAEDR
Sbjct: 825 GSFCEGSWIGEMQQASDKSVIISEILDPRTKNLLSVSKISDYVLSNELVSMALAMVAEDR 884

Query: 849 QINDVLEELFAEEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLRE 908
           QINDVLEELFAE                                QGNE+ IR ADLYLRE
Sbjct: 885 QINDVLEELFAE--------------------------------QGNEMQIRPADLYLRE 893

Query: 909 GEELSFYEVLLRARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
            EEL+F+EV+LR RQR+EIVIGYR  +AERA+INPP K  RR+WS  DVFVVI EKE
Sbjct: 945 DEELNFFEVMLRGRQRKEIVIGYRLVDAERAIINPPDKVSRRRWSAKDVFVVITEKE 893

BLAST of Lsi04G007020 vs. ExPASy Swiss-Prot
Match: Q6RHR6 (Ion channel DMI1 OS=Medicago truncatula OX=3880 GN=DMI1 PE=1 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 1.3e-270
Identity = 524/876 (59.82%), Postives = 616/876 (70.32%), Query Frame = 0

Query: 98  SSSAKKVSDTSRLSRPIDSTLKVRWHFL---AIAVSTRISKL-NIKLRACNLFDSGSEDD 157
           SSS  K       S PI   L +    L   +  +  +++KL ++KL+ C   D  S + 
Sbjct: 115 SSSITKQQQQHSTSSPIFYLLVICCIILVPYSAYLQYKLAKLKDMKLQLCGQIDFCSRNG 174

Query: 158 VRSADELADVVTDKRLKALALIASLTLLLAPIIILKYIDY----ISKSRSLDHNLEEVSL 217
             S  E  D   +   + +AL   L  L+ P ++ KY+DY    I+  R  + N E+V L
Sbjct: 175 KTSIQEEVDDDDNADSRTIALYIVLFTLILPFVLYKYLDYLPQIINFLRRTESNKEDVPL 234

Query: 218 NKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADA 277
            K++AY VDVFFSI+PYAK LALL ATL LI  GGLAL+ VT  S+ + LW SWTYVADA
Sbjct: 235 KKRVAYMVDVFFSIYPYAKLLALLCATLFLIAFGGLALYAVTGGSMAEALWHSWTYVADA 294

Query: 278 GNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLIL 337
           GNHA +EG G R+VSVS+S GGMLIFAMMLGLVSD+ISEK DSLRKG+SEV+E+NH LIL
Sbjct: 295 GNHAETEGTGQRIVSVSISAGGMLIFAMMLGLVSDAISEKVDSLRKGKSEVIERNHVLIL 354

Query: 338 GWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPL 397
           GWSDKLGSLL Q++IAN+S+GGG +VV+AE++KEEME+DIAK+EFDF GTSVICRSGSPL
Sbjct: 355 GWSDKLGSLLKQLAIANKSVGGGVIVVLAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPL 414

Query: 398 ILADLKKTHTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQS 457
           ILADLKK                                VSVSKARAIIV+A D NADQS
Sbjct: 415 ILADLKK--------------------------------VSVSKARAIIVLAADENADQS 474

Query: 458 DARALRTVLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCA 517
           DARALR VLSL GVKEGL GH+VVE+SDLDNE LVKLVGGEL+ETVVAHDVIGRLMIQCA
Sbjct: 475 DARALRVVLSLAGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHDVIGRLMIQCA 534

Query: 518 RQPGLAQIWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLN 577
            QPGLAQIWEDILGFEN EFYIKRWP+L  + F+D+LISFPDAIPCG+KVA+ GGKIV+N
Sbjct: 535 LQPGLAQIWEDILGFENAEFYIKRWPELDDLLFKDILISFPDAIPCGVKVAADGGKIVIN 594

Query: 578 PEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDD 637
           P+D+YVL++GDEVLVIAEDDDTYAP  LP VR+  F  I  P + P+KIL CGWRRDIDD
Sbjct: 595 PDDNYVLRDGDEVLVIAEDDDTYAPGPLPEVRKGYFPRIRDPPKYPEKILFCGWRRDIDD 654

Query: 638 MIVVWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELW 697
           MI+                                            VL+AFLAPGSELW
Sbjct: 655 MIM--------------------------------------------VLEAFLAPGSELW 714

Query: 698 MFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADES 757
           MFN+VPE ERE+KL  G LD+  LENI LV REGNAVIRRHLESLPLE+FDSILILADES
Sbjct: 715 MFNEVPEKERERKLAAGELDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILILADES 774

Query: 758 VEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTA-HGGSFSQGSWIGEMQQASDKSVI 817
           VEDS   +DSRSLATLLLIRDIQ++R+P RD K+T+     FS  SWI EMQQASDKS+I
Sbjct: 775 VEDSVAHSDSRSLATLLLIRDIQSRRLPYRDTKSTSLRLSGFSHNSWIREMQQASDKSII 834

Query: 818 ISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIIS 877
           ISEILD RT+NL+S+S+ISDYVLSNELVSMALAMVAED+QIN VLEELFAEE        
Sbjct: 835 ISEILDSRTRNLVSVSRISDYVLSNELVSMALAMVAEDKQINRVLEELFAEE-------- 882

Query: 878 VLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVI 937
                                   GNE+ I+ A+ YL + EEL FY++++R R R+EIVI
Sbjct: 895 ------------------------GNEMCIKPAEFYLFDQEELCFYDIMIRGRTRKEIVI 882

Query: 938 GYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           GYR AN ERA+INP  K+  RKWS+ DVFVV+A  E
Sbjct: 955 GYRLANQERAIINPSEKSVPRKWSLDDVFVVLASGE 882

BLAST of Lsi04G007020 vs. ExPASy Swiss-Prot
Match: Q4VY51 (Probable ion channel SYM8 OS=Pisum sativum OX=3888 GN=SYM8 PE=1 SV=3)

HSP 1 Score: 930.6 bits (2404), Expect = 1.4e-269
Identity = 516/846 (60.99%), Postives = 610/846 (72.10%), Query Frame = 0

Query: 132 RISKL-NIKLRACNLFD--SGS-----EDDVRSADELADVVTDKRLKALALIASLTLLLA 191
           +++KL ++KL+ C   D  SG+     + DV      +  + +   + ++L   L  L+ 
Sbjct: 157 KLAKLKDMKLQLCCQIDFCSGNGKTSLQKDVVDDGSFSYYILNADSRTISLYIVLFTLVL 216

Query: 192 PIIILKYIDY----ISKSRSLDHNLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLL 251
           P I+ KYIDY    I+ SR  + N E+V L K++AY VDVFFSI+PYAK LALL ATL L
Sbjct: 217 PFILYKYIDYLPQMINFSRRTNSNKEDVPLKKRVAYMVDVFFSIYPYAKLLALLFATLFL 276

Query: 252 IMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSEGIGPRLVSVSVSFGGMLIFAMML 311
           I  GGLAL+ VT  S+ + LW SWTYVADAGNHA +EG+G R+VSVS+S GGMLIFAMML
Sbjct: 277 IAFGGLALYAVTGGSMAEALWHSWTYVADAGNHAETEGMGQRIVSVSISAGGMLIFAMML 336

Query: 312 GLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAE 371
           GLVSD+ISEK DSLRKG+SEV+E+NH LILGWSDKLGSLL Q++IAN+S+GGG +VV+AE
Sbjct: 337 GLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQLAIANKSVGGGVIVVLAE 396

Query: 372 RDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHTMSFWSMNTINFQLRISLFSL 431
           ++KEEME+DIAK+EFDF GTSVICRSGSPLILADLKK                       
Sbjct: 397 KEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKK----------------------- 456

Query: 432 EVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLGGHIVVELSDLD 491
                    VSVSKARAIIV+A D NADQSDARALR VLSLTGVKE L GH+VVE+SDLD
Sbjct: 457 ---------VSVSKARAIIVLASDENADQSDARALRVVLSLTGVKEALRGHVVVEMSDLD 516

Query: 492 NEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLVG 551
           NE LVKLVGGEL+ETVVAHDVIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+L G
Sbjct: 517 NEPLVKLVGGELIETVVAHDVIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDG 576

Query: 552 MQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPT 611
           + F+D+LISFPDAIPCG+KV++ GGKIV+NP+D+YVL++GDEVLVIAEDDDTYAP  LP 
Sbjct: 577 LLFKDILISFPDAIPCGVKVSADGGKIVINPDDNYVLRDGDEVLVIAEDDDTYAPGPLPE 636

Query: 612 VREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKNFIVPKSAERILLCGWRRD 671
           VR+  F  I  P + P+KIL CGWRRDIDDMI+                           
Sbjct: 637 VRKGYFPRIRDPPKYPEKILFCGWRRDIDDMIM--------------------------- 696

Query: 672 MEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLV 731
                            VL+AFLAPGSELWMFN+VPE +RE+KL  G LD+  LENI LV
Sbjct: 697 -----------------VLEAFLAPGSELWMFNEVPEKQRERKLAAGELDVFGLENIKLV 756

Query: 732 DREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR 791
            REGNAVIRRHLESLPLE+FDSILILADESVEDS   +DSRSLATLLLIRDIQ++R+P R
Sbjct: 757 HREGNAVIRRHLESLPLETFDSILILADESVEDSVAHSDSRSLATLLLIRDIQSRRLPYR 816

Query: 792 DAKTTA-HGGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSM 851
           D K+T+     FS  SWI EMQQASDKS+IISEILD RT+NL+S+S+ISDYVLSNELVSM
Sbjct: 817 DTKSTSLRLSGFSHNSWIREMQQASDKSIIISEILDSRTRNLVSVSRISDYVLSNELVSM 876

Query: 852 ALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHI 911
           ALAMVAED+QIN VLEELFAEE                                GNE+ I
Sbjct: 877 ALAMVAEDKQINRVLEELFAEE--------------------------------GNEMCI 894

Query: 912 RQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFV 965
           + A+ YL + EEL FY++++R R R+EIVIGYR A+ ERA+INP  K+  RKWS+ DVFV
Sbjct: 937 KPAEFYLFDQEELCFYDIMIRGRTRKEIVIGYRLASQERALINPSEKSMTRKWSLDDVFV 894

BLAST of Lsi04G007020 vs. ExPASy Swiss-Prot
Match: Q5H8A5 (Ion channel POLLUX OS=Lotus japonicus OX=34305 GN=POLLUX PE=1 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 2.2e-267
Identity = 503/797 (63.11%), Postives = 585/797 (73.40%), Query Frame = 0

Query: 170 KALALIASLTLLLAPIIILKYIDY----ISKSRSLDHNLEEVSLNKQLAYKVDVFFSIHP 229
           KA   I   TL+L P ++ KY+DY    I+  R   +N E+V L K++AY +DVFFSI+P
Sbjct: 227 KAALYIVLFTLIL-PFLLYKYLDYLPQIINFLRRTHNNKEDVPLKKRIAYMLDVFFSIYP 286

Query: 230 YAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSEGIGPRLVSV 289
           YAK LALL ATL LI  GGLAL+ VT  SL + LW SWTYVAD+GNHA ++G G R+VSV
Sbjct: 287 YAKLLALLFATLFLIGFGGLALYAVTGGSLAEALWHSWTYVADSGNHAETQGTGQRVVSV 346

Query: 290 SVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLNQISIA 349
           S+S GGMLIFAMMLGLVSD+ISEK DSLRKG+ EV+E+NH LILGWSDKLGSLL Q++IA
Sbjct: 347 SISSGGMLIFAMMLGLVSDAISEKVDSLRKGKCEVIERNHILILGWSDKLGSLLKQLAIA 406

Query: 350 NESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHTMSFWSM 409
           N+S+GGG +VV+AE++KEEME+DI K+EFDF GTSVICRSGSPLILADLKK         
Sbjct: 407 NKSVGGGVIVVLAEKEKEEMEMDITKLEFDFMGTSVICRSGSPLILADLKK--------- 466

Query: 410 NTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKE 469
                                  VSVSKARAIIV+A D NADQSDARALR VLSLTGVKE
Sbjct: 467 -----------------------VSVSKARAIIVLASDENADQSDARALRVVLSLTGVKE 526

Query: 470 GLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFE 529
           GL GH+VVE+SDLDNE LVKLVGGEL+ETVVAHDVIGRLMIQCA QPGLAQIWEDILGFE
Sbjct: 527 GLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHDVIGRLMIQCALQPGLAQIWEDILGFE 586

Query: 530 NCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVI 589
           N EFYIKRWP+L G+ F+D+LISFPDAIPCG+KVA+ GGKIV+NP+DSYV+++GDEVLVI
Sbjct: 587 NAEFYIKRWPELDGLSFKDILISFPDAIPCGVKVAADGGKIVINPDDSYVMRDGDEVLVI 646

Query: 590 AEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKNFIVP 649
           AEDDDTY+P +LP V +  F  I    + P+KIL CGWRRDIDDMI+             
Sbjct: 647 AEDDDTYSPGSLPEVLKGFFPRIPDAPKYPEKILFCGWRRDIDDMIM------------- 706

Query: 650 KSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPENEREKKLVD 709
                                          VL+AFLAPGSELWMFN+VPE EREKKL  
Sbjct: 707 -------------------------------VLEAFLAPGSELWMFNEVPEKEREKKLAA 766

Query: 710 GGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATL 769
           GGLD+  LENI LV REGNAVIRRHLESLPLE+FDSILILADESVEDS   +DSRSLATL
Sbjct: 767 GGLDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILILADESVEDSVAHSDSRSLATL 826

Query: 770 LLIRDIQAKRMPVRDAKTTA-HGGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMS 829
           LLIRDIQ++R+P +D K+T+     FS  SWI EMQQASDKS+IISEILD RT+NL+S+S
Sbjct: 827 LLIRDIQSRRLPYKDTKSTSLRLSGFSHNSWIREMQQASDKSIIISEILDSRTRNLVSVS 886

Query: 830 KISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMRLLLWHTHN 889
           +ISDYVLSNELVSMALAMVAED+QIN VLEELFAE                         
Sbjct: 887 RISDYVLSNELVSMALAMVAEDKQINRVLEELFAE------------------------- 914

Query: 890 LLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAERAVINPPA 949
                  QGNE+ I+ A+ YL + EEL FY++++R R R+EI+IGYR AN ERA+INP  
Sbjct: 947 -------QGNEMCIKPAEFYLFDQEELCFYDIMIRGRARQEIIIGYRLANQERAIINPSE 914

Query: 950 KNERRKWSVGDVFVVIA 962
           K   RKWS+GDVFVVIA
Sbjct: 1007 KLVARKWSLGDVFVVIA 914

BLAST of Lsi04G007020 vs. ExPASy TrEMBL
Match: A0A5A7SU61 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001710 PE=3 SV=1)

HSP 1 Score: 1579.3 bits (4088), Expect = 0.0e+00
Identity = 848/988 (85.83%), Postives = 865/988 (87.55%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HS+PAKS NYIRRFS+TSR+SRRYTDY RYRKTSS ISDSHSCSTI NDV
Sbjct: 14  DWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCISDSHSCSTITNDV 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFAR+RRRF FD RSDLSLKRSEVEFSSK KLELPDVSSSAKKVSDTSRLS+  DS+LKV
Sbjct: 74  KFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDTSRLSKSFDSSLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                        TRIS LNIKLRACNLFD G+EDDVRS
Sbjct: 134 RWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRACNLFDHGNEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELADVVTDKRLK LALIASLTLL APIIILKYIDYISKSRSLDHNLEE+SLNK+LAYK
Sbjct: 194 PDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHNLEEISLNKRLAYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           G GPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAIIVIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIIVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 660
           +EGDEVLVIAEDDDTYAPA LPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG
Sbjct: 614 EEGDEVLVIAEDDDTYAPAPLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 673

Query: 661 SLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 720
           SLPK+FIVPKSAERILLCGWRRDMEDMIM           VLDAFLAPGSELWMFNDVPE
Sbjct: 674 SLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VLDAFLAPGSELWMFNDVPE 733

Query: 721 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 780
           NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ
Sbjct: 734 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 793

Query: 781 ADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASDKSVIISEILDPR 840
           ADSRSLATLLLIRDIQAKRMPVRDAK TAH GSFSQGSWIGEMQQASDKSVIISEILDPR
Sbjct: 794 ADSRSLATLLLIRDIQAKRMPVRDAKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPR 853

Query: 841 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMR 900
           TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE                
Sbjct: 854 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---------------- 913

Query: 901 LLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAE 960
                           GNELHIRQADLYLREGEELSFYEVLLRARQRRE+VIGYRSANAE
Sbjct: 914 ----------------GNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAE 926

Query: 961 RAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RAVINPPAKNERRKWS+ DVFVVIAEKE
Sbjct: 974 RAVINPPAKNERRKWSLRDVFVVIAEKE 926

BLAST of Lsi04G007020 vs. ExPASy TrEMBL
Match: A0A5D3CV00 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002430 PE=3 SV=1)

HSP 1 Score: 1570.4 bits (4065), Expect = 0.0e+00
Identity = 848/1000 (84.80%), Postives = 865/1000 (86.50%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HS+PAKS NYIRRFS+TSR+SRRYTDY RYRKTSS ISDSHSCSTI NDV
Sbjct: 14  DWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCISDSHSCSTITNDV 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFAR+RRRF FD RSDLSLKRSEVEFSSK KLELPDVSSSAKKVSDTSRLS+  DS+LKV
Sbjct: 74  KFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDTSRLSKSFDSSLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                        TRIS LNIKLRACNLFD G+EDDVRS
Sbjct: 134 RWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRACNLFDHGNEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELADVVTDKRLK LALIASLTLL APIIILKYIDYISKSRSLDHNLEE+SLNK+LAYK
Sbjct: 194 PDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHNLEEISLNKRLAYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           G GPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAIIVIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIIVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 660
           +EGDEVLVIAEDDDTYAPA LPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG
Sbjct: 614 EEGDEVLVIAEDDDTYAPAPLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 673

Query: 661 SLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 720
           SLPK+FIVPKSAERILLCGWRRDMEDMIM           VLDAFLAPGSELWMFNDVPE
Sbjct: 674 SLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VLDAFLAPGSELWMFNDVPE 733

Query: 721 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS------------ILI 780
           NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS            ILI
Sbjct: 734 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSVSSVISFHPGSEILI 793

Query: 781 LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASD 840
           LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAK TAH GSFSQGSWIGEMQQASD
Sbjct: 794 LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKATAHKGSFSQGSWIGEMQQASD 853

Query: 841 KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNI 900
           KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE    
Sbjct: 854 KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---- 913

Query: 901 PIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRR 960
                                       GNELHIRQADLYLREGEELSFYEVLLRARQRR
Sbjct: 914 ----------------------------GNELHIRQADLYLREGEELSFYEVLLRARQRR 938

Query: 961 EIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           E+VIGYRSANAERAVINPPAKNERRKWS+ DVFVVIAEKE
Sbjct: 974 EVVIGYRSANAERAVINPPAKNERRKWSLRDVFVVIAEKE 938

BLAST of Lsi04G007020 vs. ExPASy TrEMBL
Match: A0A6J1GWH3 (ion channel CASTOR-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458157 PE=3 SV=1)

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 836/1006 (83.10%), Postives = 854/1006 (84.89%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HSYPAKS  YIRRFSETSRISRRY D+QRYRK+SS ISDSHSCS+  ND 
Sbjct: 14  DWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCISDSHSCSSTSNDA 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFARTRRR DFD RSDLSLKRSE EFSSKRKLE  DVSSSAKKVSDTSRL R  DSTLKV
Sbjct: 74  KFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDTSRLFRSFDSTLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                         RIS LNIKLRACNLFDSGSEDDVRS
Sbjct: 134 RWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRACNLFDSGSEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELAD + DKRLK LALIASLTLLLAPI+ILKYID  SKSRSL+HNLEEVSLNKQL YK
Sbjct: 194 PDELADFM-DKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHNLEEVSLNKQLLYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAI+VIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIVVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 660
           QEGDEVLVIAEDDDTYAPAALPTV E SFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 QEGDEVLVIAEDDDTYAPAALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAF 673

Query: 661 --------------VWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVL 720
                         VWRGSLPK+FIVPKSAERILLCGWRRDMEDMIM           VL
Sbjct: 674 IKALILSFWHWMLQVWRGSLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VL 733

Query: 721 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 780
           DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES
Sbjct: 734 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 793

Query: 781 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGE 840
           FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR AKT A+ GS SQGSWIGE
Sbjct: 794 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRGAKTVAYRGSVSQGSWIGE 853

Query: 841 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 900
           MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA
Sbjct: 854 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 913

Query: 901 EEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLL 960
           EE                                GNELHIRQADLYLREGEELSFYEVLL
Sbjct: 914 EE--------------------------------GNELHIRQADLYLREGEELSFYEVLL 941

Query: 961 RARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RARQRREIVIGYRSA+AERAVINPPAK+ERRKW +GDVFVVIAEKE
Sbjct: 974 RARQRREIVIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Lsi04G007020 vs. ExPASy TrEMBL
Match: A0A6J1K6V9 (ion channel CASTOR-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492175 PE=3 SV=1)

HSP 1 Score: 1530.0 bits (3960), Expect = 0.0e+00
Identity = 835/1006 (83.00%), Postives = 852/1006 (84.69%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSFV SYPAKSQ YIRRFSETSRISRRY D+QRYRK+SS ISDSHSCS+  ND 
Sbjct: 14  DWFFPPQSFVQSYPAKSQTYIRRFSETSRISRRYADHQRYRKSSSCISDSHSCSSTSNDA 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFARTRRR DFD RSDLSLKRSE+EFSSKRKLE  DVSSSAKKVSDTSRL R  DSTLKV
Sbjct: 74  KFARTRRRIDFDRRSDLSLKRSELEFSSKRKLEQSDVSSSAKKVSDTSRLFRSFDSTLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                         RIS LNIKLRACNLFDSGSEDDVRS
Sbjct: 134 RWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRACNLFDSGSEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELAD + DKRLK LALI SLTLLLAPIIILKYID  SKSRSL+HNLEE+SLNKQL YK
Sbjct: 194 PDELADFM-DKRLKTLALITSLTLLLAPIIILKYID--SKSRSLEHNLEEISLNKQLLYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFS+HPYAKPLALLIATL LIMLGGLALFGVTDDSLVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSVHPYAKPLALLIATLQLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAI+VIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIVVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 660
           QEGDEVLVIAEDDDTYAPAALPTV E SFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 QEGDEVLVIAEDDDTYAPAALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAF 673

Query: 661 --------------VWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVL 720
                         VWRGSLPK+FIVPKSAERILLCGWRRDMEDMIM           VL
Sbjct: 674 IKALILSFWHWMLQVWRGSLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VL 733

Query: 721 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 780
           DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES
Sbjct: 734 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 793

Query: 781 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGE 840
           FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR AKT  + GS SQGSWIGE
Sbjct: 794 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRGAKTVTYRGSVSQGSWIGE 853

Query: 841 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 900
           MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA
Sbjct: 854 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 913

Query: 901 EEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLL 960
           EE                                GNELHIRQADLYLREGEELSFYEVLL
Sbjct: 914 EE--------------------------------GNELHIRQADLYLREGEELSFYEVLL 941

Query: 961 RARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RARQRREIVIGYRSA+AERAVINPPAK+ERRKW +GDVFVVIAEKE
Sbjct: 974 RARQRREIVIGYRSAHAERAVINPPAKHERRKWYLGDVFVVIAEKE 941

BLAST of Lsi04G007020 vs. ExPASy TrEMBL
Match: A0A1S3BEQ8 (ion channel CASTOR-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488869 PE=3 SV=1)

HSP 1 Score: 1497.3 bits (3875), Expect = 0.0e+00
Identity = 816/988 (82.59%), Postives = 832/988 (84.21%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HS+PAKS NYIRRFS+TSR+SRRYTDY RYRKTSS ISDSHSCSTI NDV
Sbjct: 14  DWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCISDSHSCSTITNDV 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFAR+RRRF FD RSDLSLKRSEVEFSSK KLELPDVSSSAKKVSDTSRLS+  DS+LKV
Sbjct: 74  KFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDTSRLSKSFDSSLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                        TRIS LNIKLRACNLFD G+EDDVRS
Sbjct: 134 RWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRACNLFDHGNEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELADVVTDKRLK LALIASLTLL APIIILKYIDYISKSRSLDHNLEE+SLNK+LAYK
Sbjct: 194 PDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHNLEEISLNKRLAYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           G GPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAIIVIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIIVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 660
           +EGDEVLVIAEDDDTYAPA LPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 EEGDEVLVIAEDDDTYAPAPLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 673

Query: 661 SLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 720
                                                   VLDAFLAPGSELWMFNDVPE
Sbjct: 674 ----------------------------------------VLDAFLAPGSELWMFNDVPE 733

Query: 721 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 780
           NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ
Sbjct: 734 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 793

Query: 781 ADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASDKSVIISEILDPR 840
           ADSRSLATLLLIRDIQAKRMPVRDAK TAH GSFSQGSWIGEMQQASDKSVIISEILDPR
Sbjct: 794 ADSRSLATLLLIRDIQAKRMPVRDAKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPR 853

Query: 841 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMR 900
           TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE                
Sbjct: 854 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---------------- 893

Query: 901 LLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAE 960
                           GNELHIRQADLYLREGEELSFYEVLLRARQRRE+VIGYRSANAE
Sbjct: 914 ----------------GNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAE 893

Query: 961 RAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RAVINPPAKNERRKWS+ DVFVVIAEKE
Sbjct: 974 RAVINPPAKNERRKWSLRDVFVVIAEKE 893

BLAST of Lsi04G007020 vs. NCBI nr
Match: KAA0034178.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1579.3 bits (4088), Expect = 0.0e+00
Identity = 848/988 (85.83%), Postives = 865/988 (87.55%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HS+PAKS NYIRRFS+TSR+SRRYTDY RYRKTSS ISDSHSCSTI NDV
Sbjct: 14  DWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCISDSHSCSTITNDV 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFAR+RRRF FD RSDLSLKRSEVEFSSK KLELPDVSSSAKKVSDTSRLS+  DS+LKV
Sbjct: 74  KFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDTSRLSKSFDSSLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                        TRIS LNIKLRACNLFD G+EDDVRS
Sbjct: 134 RWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRACNLFDHGNEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELADVVTDKRLK LALIASLTLL APIIILKYIDYISKSRSLDHNLEE+SLNK+LAYK
Sbjct: 194 PDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHNLEEISLNKRLAYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           G GPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAIIVIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIIVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 660
           +EGDEVLVIAEDDDTYAPA LPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG
Sbjct: 614 EEGDEVLVIAEDDDTYAPAPLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 673

Query: 661 SLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 720
           SLPK+FIVPKSAERILLCGWRRDMEDMIM           VLDAFLAPGSELWMFNDVPE
Sbjct: 674 SLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VLDAFLAPGSELWMFNDVPE 733

Query: 721 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 780
           NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ
Sbjct: 734 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQ 793

Query: 781 ADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASDKSVIISEILDPR 840
           ADSRSLATLLLIRDIQAKRMPVRDAK TAH GSFSQGSWIGEMQQASDKSVIISEILDPR
Sbjct: 794 ADSRSLATLLLIRDIQAKRMPVRDAKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPR 853

Query: 841 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMR 900
           TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE                
Sbjct: 854 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---------------- 913

Query: 901 LLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAE 960
                           GNELHIRQADLYLREGEELSFYEVLLRARQRRE+VIGYRSANAE
Sbjct: 914 ----------------GNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAE 926

Query: 961 RAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RAVINPPAKNERRKWS+ DVFVVIAEKE
Sbjct: 974 RAVINPPAKNERRKWSLRDVFVVIAEKE 926

BLAST of Lsi04G007020 vs. NCBI nr
Match: TYK15741.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1570.4 bits (4065), Expect = 0.0e+00
Identity = 848/1000 (84.80%), Postives = 865/1000 (86.50%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HS+PAKS NYIRRFS+TSR+SRRYTDY RYRKTSS ISDSHSCSTI NDV
Sbjct: 14  DWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCISDSHSCSTITNDV 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFAR+RRRF FD RSDLSLKRSEVEFSSK KLELPDVSSSAKKVSDTSRLS+  DS+LKV
Sbjct: 74  KFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDTSRLSKSFDSSLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                        TRIS LNIKLRACNLFD G+EDDVRS
Sbjct: 134 RWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRACNLFDHGNEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELADVVTDKRLK LALIASLTLL APIIILKYIDYISKSRSLDHNLEE+SLNK+LAYK
Sbjct: 194 PDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHNLEEISLNKRLAYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           G GPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAIIVIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIIVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 660
           +EGDEVLVIAEDDDTYAPA LPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG
Sbjct: 614 EEGDEVLVIAEDDDTYAPAPLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRG 673

Query: 661 SLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 720
           SLPK+FIVPKSAERILLCGWRRDMEDMIM           VLDAFLAPGSELWMFNDVPE
Sbjct: 674 SLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VLDAFLAPGSELWMFNDVPE 733

Query: 721 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS------------ILI 780
           NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS            ILI
Sbjct: 734 NEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSVSSVISFHPGSEILI 793

Query: 781 LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASD 840
           LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAK TAH GSFSQGSWIGEMQQASD
Sbjct: 794 LADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKATAHKGSFSQGSWIGEMQQASD 853

Query: 841 KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALNI 900
           KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE    
Sbjct: 854 KSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEE---- 913

Query: 901 PIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQRR 960
                                       GNELHIRQADLYLREGEELSFYEVLLRARQRR
Sbjct: 914 ----------------------------GNELHIRQADLYLREGEELSFYEVLLRARQRR 938

Query: 961 EIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           E+VIGYRSANAERAVINPPAKNERRKWS+ DVFVVIAEKE
Sbjct: 974 EVVIGYRSANAERAVINPPAKNERRKWSLRDVFVVIAEKE 938

BLAST of Lsi04G007020 vs. NCBI nr
Match: XP_022956408.1 (ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956409.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956410.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956411.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956412.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 836/1006 (83.10%), Postives = 854/1006 (84.89%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HSYPAKS  YIRRFSETSRISRRY D+QRYRK+SS ISDSHSCS+  ND 
Sbjct: 14  DWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCISDSHSCSSTSNDA 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFARTRRR DFD RSDLSLKRSE EFSSKRKLE  DVSSSAKKVSDTSRL R  DSTLKV
Sbjct: 74  KFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDTSRLFRSFDSTLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                         RIS LNIKLRACNLFDSGSEDDVRS
Sbjct: 134 RWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRACNLFDSGSEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELAD + DKRLK LALIASLTLLLAPI+ILKYID  SKSRSL+HNLEEVSLNKQL YK
Sbjct: 194 PDELADFM-DKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHNLEEVSLNKQLLYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAI+VIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIVVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 660
           QEGDEVLVIAEDDDTYAPAALPTV E SFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 QEGDEVLVIAEDDDTYAPAALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAF 673

Query: 661 --------------VWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVL 720
                         VWRGSLPK+FIVPKSAERILLCGWRRDMEDMIM           VL
Sbjct: 674 IKALILSFWHWMLQVWRGSLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VL 733

Query: 721 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 780
           DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES
Sbjct: 734 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 793

Query: 781 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGE 840
           FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR AKT A+ GS SQGSWIGE
Sbjct: 794 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRGAKTVAYRGSVSQGSWIGE 853

Query: 841 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 900
           MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA
Sbjct: 854 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 913

Query: 901 EEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLL 960
           EE                                GNELHIRQADLYLREGEELSFYEVLL
Sbjct: 914 EE--------------------------------GNELHIRQADLYLREGEELSFYEVLL 941

Query: 961 RARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RARQRREIVIGYRSA+AERAVINPPAK+ERRKW +GDVFVVIAEKE
Sbjct: 974 RARQRREIVIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Lsi04G007020 vs. NCBI nr
Match: KAG6601611.1 (Ion channel CASTOR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 836/1006 (83.10%), Postives = 854/1006 (84.89%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSF+HSYPAKS  YIRRFSETSRISRRY D+QRYRK+SS ISDSHSCS+  ND 
Sbjct: 14  DWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCISDSHSCSSTSNDA 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFARTRRR DFD RSDLSLKRSE EFSSKRKLE  DVSSSAKKVSDTSRL R  DSTLKV
Sbjct: 74  KFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDTSRLFRSFDSTLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                         RIS LNIKLRACNLFDSGSEDDVRS
Sbjct: 134 RWRFLAIAASIFLVIFATLLHENSSLQEQVNGLEARISNLNIKLRACNLFDSGSEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELAD + DKRLK LALIASLTLLLAPI+ILKYID  SKSRSL+HNLEEVSLNKQL YK
Sbjct: 194 PDELADFM-DKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHNLEEVSLNKQLLYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICR+GSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRTGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAI+VIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIVVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 660
           QEGDEVLVIAEDDDTYAPAALPTV E SFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 QEGDEVLVIAEDDDTYAPAALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAF 673

Query: 661 --------------VWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVL 720
                         VWRGSLPK+FIVPKSAERILLCGWRRDMEDMIM           VL
Sbjct: 674 IKALILSFWHWMLQVWRGSLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VL 733

Query: 721 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 780
           DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES
Sbjct: 734 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 793

Query: 781 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGE 840
           FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR AKT A+ GS SQGSWIGE
Sbjct: 794 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRGAKTVAYRGSVSQGSWIGE 853

Query: 841 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 900
           MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA
Sbjct: 854 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 913

Query: 901 EEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLL 960
           EE                                GNELHIRQADLYLREGEELSFYEVLL
Sbjct: 914 EE--------------------------------GNELHIRQADLYLREGEELSFYEVLL 941

Query: 961 RARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RARQRREIVIGYRSA+AERAVINPPAK+ERRKW +GDVFVVIAEKE
Sbjct: 974 RARQRREIVIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Lsi04G007020 vs. NCBI nr
Match: XP_022997206.1 (ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997214.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997221.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997229.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1530.0 bits (3960), Expect = 0.0e+00
Identity = 835/1006 (83.00%), Postives = 852/1006 (84.69%), Query Frame = 0

Query: 1   DWFFPPQSFVHSYPAKSQNYIRRFSETSRISRRYTDYQRYRKTSSSISDSHSCSTIGNDV 60
           DWFFPPQSFV SYPAKSQ YIRRFSETSRISRRY D+QRYRK+SS ISDSHSCS+  ND 
Sbjct: 14  DWFFPPQSFVQSYPAKSQTYIRRFSETSRISRRYADHQRYRKSSSCISDSHSCSSTSNDA 73

Query: 61  KFARTRRRFDFDHRSDLSLKRSEVEFSSKRKLELPDVSSSAKKVSDTSRLSRPIDSTLKV 120
           KFARTRRR DFD RSDLSLKRSE+EFSSKRKLE  DVSSSAKKVSDTSRL R  DSTLKV
Sbjct: 74  KFARTRRRIDFDRRSDLSLKRSELEFSSKRKLEQSDVSSSAKKVSDTSRLFRSFDSTLKV 133

Query: 121 RWHFLAIAVS------------------------TRISKLNIKLRACNLFDSGSEDDVRS 180
           RW FLAIA S                         RIS LNIKLRACNLFDSGSEDDVRS
Sbjct: 134 RWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRACNLFDSGSEDDVRS 193

Query: 181 ADELADVVTDKRLKALALIASLTLLLAPIIILKYIDYISKSRSLDHNLEEVSLNKQLAYK 240
            DELAD + DKRLK LALI SLTLLLAPIIILKYID  SKSRSL+HNLEE+SLNKQL YK
Sbjct: 194 PDELADFM-DKRLKTLALITSLTLLLAPIIILKYID--SKSRSLEHNLEEISLNKQLLYK 253

Query: 241 VDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSE 300
           VDVFFS+HPYAKPLALLIATL LIMLGGLALFGVTDDSLVDCLWLSWTYVAD+GNHANSE
Sbjct: 254 VDVFFSVHPYAKPLALLIATLQLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSE 313

Query: 301 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLG 360
           GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQNHTLILGWSDKLG
Sbjct: 314 GIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQNHTLILGWSDKLG 373

Query: 361 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 420
           SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK
Sbjct: 374 SLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKK 433

Query: 421 THTMSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRT 480
                                           VSVSKARAI+VIAEDGNADQSDARALRT
Sbjct: 434 --------------------------------VSVSKARAIVVIAEDGNADQSDARALRT 493

Query: 481 VLSLTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 540
           VLSLTGVKEGL GHIVVELSDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ
Sbjct: 494 VLSLTGVKEGLRGHIVVELSDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQ 553

Query: 541 IWEDILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 600
           IWEDILGFENCEFYIKRWPQL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL
Sbjct: 554 IWEDILGFENCEFYIKRWPQLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL 613

Query: 601 QEGDEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIV---- 660
           QEGDEVLVIAEDDDTYAPAALPTV E SFIHIARPTRKPQKILLCGWRRDIDDMIV    
Sbjct: 614 QEGDEVLVIAEDDDTYAPAALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAF 673

Query: 661 --------------VWRGSLPKNFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVL 720
                         VWRGSLPK+FIVPKSAERILLCGWRRDMEDMIM           VL
Sbjct: 674 IKALILSFWHWMLQVWRGSLPKDFIVPKSAERILLCGWRRDMEDMIM-----------VL 733

Query: 721 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 780
           DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES
Sbjct: 734 DAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAVIRRHLESLPLES 793

Query: 781 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGE 840
           FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR AKT  + GS SQGSWIGE
Sbjct: 794 FDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRGAKTVTYRGSVSQGSWIGE 853

Query: 841 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 900
           MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA
Sbjct: 854 MQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFA 913

Query: 901 EEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLL 960
           EE                                GNELHIRQADLYLREGEELSFYEVLL
Sbjct: 914 EE--------------------------------GNELHIRQADLYLREGEELSFYEVLL 941

Query: 961 RARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RARQRREIVIGYRSA+AERAVINPPAK+ERRKW +GDVFVVIAEKE
Sbjct: 974 RARQRREIVIGYRSAHAERAVINPPAKHERRKWYLGDVFVVIAEKE 941

BLAST of Lsi04G007020 vs. TAIR 10
Match: AT5G49960.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1012 (InterPro:IPR010420); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF1012) (TAIR:AT5G02940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 870.2 bits (2247), Expect = 1.6e-252
Identity = 485/846 (57.33%), Postives = 588/846 (69.50%), Query Frame = 0

Query: 124 FLAIAVSTRISKLNIKLRACNLFDSGSEDDVRSADELADVVTDKRLKALALIASLTLLLA 183
           FL   VS   ++  I L  CN     +E            + +   +A+   + +   + 
Sbjct: 100 FLRSKVSRLEAENIILLTRCNSSSDNNE------------MEETNSRAVVFFSVIITFVL 159

Query: 184 PIIILKYIDYISKSRSL----DHNLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLL 243
           P ++  Y+D +S  ++L    +   E+V L K+LAY +DV FS++PYAK LALL+AT++L
Sbjct: 160 PFLLYMYLDDLSHVKNLLRRTNQKKEDVPLKKRLAYSLDVCFSVYPYAKLLALLLATVVL 219

Query: 244 IMLGGLALFGVTDDSLVDCLWLSWTYVADAGNHANSEGIGPRLVSVSVSFGGMLIFAMML 303
           I+ GGLAL+ V+D  + + LWLSWT+VAD+G+HA+  G+G R+VSV++S GGMLIFA ML
Sbjct: 220 IVYGGLALYAVSDCGVDEALWLSWTFVADSGSHADRVGVGARIVSVAISAGGMLIFATML 279

Query: 304 GLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAE 363
           GL+SD+IS+  DSLRKG+SEV+E NH LILGWSDKLGSLL Q++IAN+S+GGG VVV+AE
Sbjct: 280 GLISDAISKMVDSLRKGKSEVLESNHILILGWSDKLGSLLKQLAIANKSIGGGVVVVLAE 339

Query: 364 RDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHTMSFWSMNTINFQLRISLFSL 423
           RDKEEME DIAK EFD  GTSVICRSGSPLILADLKK                       
Sbjct: 340 RDKEEMETDIAKFEFDLMGTSVICRSGSPLILADLKK----------------------- 399

Query: 424 EVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLGGHIVVELSDLD 483
                    VSVS ARAIIV+  D NADQSDARALR VLSLTGVKEG  GH+VVE+ DLD
Sbjct: 400 ---------VSVSNARAIIVLGSDENADQSDARALRVVLSLTGVKEGWKGHVVVEMCDLD 459

Query: 484 NEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLVG 543
           NE LVKLVGGE +ETVVAHDVIGRLMIQCA QPGLAQIWEDILGFEN EFYIK+WPQL G
Sbjct: 460 NEPLVKLVGGERIETVVAHDVIGRLMIQCALQPGLAQIWEDILGFENAEFYIKKWPQLDG 519

Query: 544 MQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPT 603
             FEDVLISFP+AIPCG+KVA+  GKIVLNP D YVL+EGDE+LVIAEDDDTYAP +LP 
Sbjct: 520 YCFEDVLISFPNAIPCGVKVAA-DGKIVLNPSDDYVLKEGDEILVIAEDDDTYAPGSLPE 579

Query: 604 VREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKNFIVPKSAERILLCGWRRD 663
           VR   F  +  P + P+KIL CGWRRDIDDMI                            
Sbjct: 580 VRMCHFPKMQDPPKYPEKILFCGWRRDIDDMI---------------------------- 639

Query: 664 MEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLV 723
                            VL+A LAPGSELWMFN+VP+ EREKKL D GL+IS+L NI LV
Sbjct: 640 ----------------KVLEALLAPGSELWMFNEVPDQEREKKLTDAGLNISKLVNIKLV 699

Query: 724 DREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVR 783
            R+GNAVIRRHLESLPLE+FDSILILA++S+E+S + +DSRSLATLLLIRDIQ+KR+P +
Sbjct: 700 HRQGNAVIRRHLESLPLETFDSILILAEQSLENSIVHSDSRSLATLLLIRDIQSKRLPYK 759

Query: 784 DAKTTA-HGGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSM 843
           DAK++A     F    WI +MQQASDKS++ISEILD RTKNL+S+S+ISDYVLSNELVSM
Sbjct: 760 DAKSSALRISGFPNCCWIRKMQQASDKSIVISEILDSRTKNLVSVSRISDYVLSNELVSM 819

Query: 844 ALAMVAEDRQINDVLEELFAEEALNIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHI 903
           ALAMVAED+QIN VL+ELFAE                                +GNEL I
Sbjct: 820 ALAMVAEDKQINRVLKELFAE--------------------------------KGNELCI 824

Query: 904 RQADLYLREGEELSFYEVLLRARQRREIVIGYRSANAERAVINPPAKNERRKWSVGDVFV 963
           R A+ Y+ + EE+ FY+++ RARQR+EI+IGYR A  E+AVINP  K++  KWS+ DVFV
Sbjct: 880 RPAEFYIYDQEEVCFYDIMRRARQRQEIIIGYRLAGMEQAVINPTDKSKLTKWSLDDVFV 824

Query: 964 VIAEKE 965
           VIA  +
Sbjct: 940 VIASSQ 824

BLAST of Lsi04G007020 vs. TAIR 10
Match: AT5G43745.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 185.7 bits (470), Expect = 1.8e-46
Identity = 187/762 (24.54%), Postives = 342/762 (44.88%), Query Frame = 0

Query: 230 LALLIATLLLIMLGGLALFGVTDD-SLVDCLWLSWTYVADAGNHANSEGIGPRLVSVSVS 289
           +AL+IA +  +++GGL  F    D  L DCLW +W  +  +  H   +    R++   ++
Sbjct: 167 VALMIACVSFVIIGGLLFFKFRKDLPLEDCLWEAWACLISSSTHLKQKTRIERVIGFVLA 226

Query: 290 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQNHTLILGWSDKLGSLLNQISIANE 349
             G+L ++ +L  +++        LR+G + +V+E +H +I G +  L  +L Q++  +E
Sbjct: 227 IWGILFYSRLLSTMTEQFRYNMTKLREGAQMQVLEADHIIICGINSHLPFILKQLNSYHE 286

Query: 350 ---SLGGGT-----VVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHT 409
               LG  T     +++M++  +++M+        DF    ++ +S              
Sbjct: 287 HAVRLGTATARKQRLLLMSDTPRKQMDKLAEAYSKDFNHIDILTKS-------------- 346

Query: 410 MSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLS 469
               S+N               L  S+ + + S ARAII++   G+  + D  A  +VL+
Sbjct: 347 ---CSLN---------------LTKSFERAAASMARAIIILPTKGDRYEVDTDAFLSVLA 406

Query: 470 LTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWE 529
           L  +++      +VE+S  +   L+K + G  VE V   +V  +L +QC+RQ  L +I+ 
Sbjct: 407 LQPIQKMESIPTIVEVSSPNTYDLLKSISGLKVEPV--ENVTSKLFVQCSRQKDLIKIYR 466

Query: 530 DILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEG 589
            +L +    F +  +P LVG ++  + + F + + CG+    R GK+  +P D+  L E 
Sbjct: 467 HLLNYSKNVFNLCSFPNLVGTKYRQLRLGFQEVVVCGL---LRDGKVNFHPNDNEELMET 526

Query: 590 DEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLP 649
           D++L IA   +      L T  +   I +   TRK    +    R  +  +I+  R SL 
Sbjct: 527 DKLLFIA-PLNWKKKQLLYTDMKLENITVPTDTRKQ---VFEKKRSRLSKIIMRPRKSLS 586

Query: 650 K-NFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPENE 709
           K +  V    E ILL GWR D+  MI              D +L PGS + + +DV   +
Sbjct: 587 KGSDSVKGPTESILLLGWRGDVVQMI-----------EEFDNYLGPGSSMEILSDVSLED 646

Query: 710 REKKLVDGGLDISRLENISLVDREGN---------AVIR-----RHLESLPLESFDSILI 769
           R +  V   +   +++NI +  + GN          ++R     R  +++PL    +IL+
Sbjct: 647 RRR--VGDSIGSVKIKNIQVSHKVGNPLNYDTLKQTIMRMKSKYRKGKNIPL----TILV 706

Query: 770 LADES-VEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQAS 829
           ++D   +     +AD +S  +LLL   I   ++ V+      H                 
Sbjct: 707 ISDRDWLLGDPSRADKQSAYSLLLAESI-CNKLGVK-----VHN---------------- 766

Query: 830 DKSVIISEILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEAL 889
               + SEI+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   +  
Sbjct: 767 ----LASEIVDSKLGKQITGLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDAD-- 810

Query: 890 NIPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQ 949
                                         G+E++++  +LY++EGE  SF E+  RA  
Sbjct: 827 ------------------------------GDEIYVKDVELYMKEGENPSFTELSERAWL 810

Query: 950 RREIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RRE+ IGY      + +INP  KNE     + D  +VI+E E
Sbjct: 887 RREVAIGY--IKGGKKMINPVPKNEPLSLEMDDSLIVISELE 810

BLAST of Lsi04G007020 vs. TAIR 10
Match: AT5G02940.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 179.9 bits (455), Expect = 9.9e-45
Identity = 182/761 (23.92%), Postives = 334/761 (43.89%), Query Frame = 0

Query: 230 LALLIATLLLIMLGGLALFGV-TDDSLVDCLWLSWTYVADAGNHANSEGIGPRLVSVSVS 289
           + LLI     +++GGL  F    D SL DCLW +W  + +A  H   +    RL+   ++
Sbjct: 163 VVLLITCFSFVIIGGLFFFKFRKDTSLEDCLWEAWACLVNADTHLEQKTRFERLIGFVLA 222

Query: 290 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQNHTLILGWSDKLGSLLNQIS---- 349
             G++ ++ +L  +++        +R+G   +V+E +H +I G +  L  +L Q++    
Sbjct: 223 IWGIVFYSRLLSTMTEQFRYHMKKVREGAHMQVLESDHIIICGINSHLPFILKQLNSYQQ 282

Query: 350 ----IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKTHT 409
               +   +    T+++M++  ++EM+        DF    ++ +S              
Sbjct: 283 HAVRLGTTTARKQTLLLMSDTPRKEMDKLAEAYAKDFDQLDILTKS-------------- 342

Query: 410 MSFWSMNTINFQLRISLFSLEVLLTSYIQVSVSKARAIIVIAEDGNADQSDARALRTVLS 469
               S+N               +  S+ + +   ARAII++   G+  + D  A  +VL+
Sbjct: 343 ---CSLN---------------MTKSFERAAACMARAIIILPTKGDRYEVDTDAFLSVLA 402

Query: 470 LTGVKEGLGGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWE 529
           L  +++      +VE+S  +   L+K + G  VE V   +   +L +QC+RQ  L +I+ 
Sbjct: 403 LEPIQKMESIPTIVEVSSSNMYDLLKSISGLKVEPV--ENSTSKLFVQCSRQKDLIKIYR 462

Query: 530 DILGFENCEFYIKRWPQLVGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEG 589
            +L +    F +  +P L GM++  + + F + + CGI    R GK+  +P D   L E 
Sbjct: 463 HLLNYSKNVFNLCSFPNLTGMKYRQLRLGFQEVVVCGI---LRDGKVNFHPNDDEELMET 522

Query: 590 DEVLVIAEDDDTYAPAALPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLP 649
           D++L IA     +    + T  E   +     TRK    +    +  ++ +I     SL 
Sbjct: 523 DKLLFIAPLKKDFLYTDMKT--ENMTVDETDDTRKQ---VYEEKKSRLEKIITRPSKSLS 582

Query: 650 K---NFIVPKSAERILLCGWRRDMEDMIMVMLLRSENMLPVLDAFLAPGSELWMFNDVPE 709
           K   +F  PK  E ILL GWR D+            NM+   D++L PGS L + +DVP 
Sbjct: 583 KGSDSFKGPK--ESILLLGWRGDV-----------VNMIKEFDSYLGPGSSLEILSDVPL 642

Query: 710 NEREKKLVDGGLDISRLENISLVDREGNAV-----------IRRHLESLPLESFDSILIL 769
            +R  + VD  +   +++NI +    GN +           ++   E    +   +I+++
Sbjct: 643 EDR--RGVDQSIATGKIKNIQVSHSVGNHMDYDTLKESIMHMQNKYEKGEEDIRLTIVVI 702

Query: 770 AD-ESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKTTAHGGSFSQGSWIGEMQQASD 829
           +D + +     +AD +S  TLLL   I   ++ V+      H                  
Sbjct: 703 SDRDLLLGDPSRADKQSAYTLLLAETI-CNKLGVK-----VHN----------------- 762

Query: 830 KSVIISEILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEALN 889
              + SEI+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   E   
Sbjct: 763 ---LASEIVDTKLGKQITRLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDAE--- 806

Query: 890 IPIISVLTCSFMRLLLWHTHNLLLFCYYQGNELHIRQADLYLREGEELSFYEVLLRARQR 949
                                        G+E++++  +LY++EGE  SF E+  RA  R
Sbjct: 823 -----------------------------GDEIYVKDIELYMKEGENPSFTELSERAWLR 806

Query: 950 REIVIGYRSANAERAVINPPAKNERRKWSVGDVFVVIAEKE 965
           RE+ IGY      + +INP  K E     + D  +VI+E E
Sbjct: 883 REVAIGY--IKGGKKIINPVPKTEPVSLEMEDSLIVISELE 806