Lsi04G006850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G006850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	alpha-ketoglutarate-dependent dioxygenase alkB
Location	chr04: 6276120 .. 6276427 (-)
RNA-Seq Expression	Lsi04G006850
Synteny	Lsi04G006850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGTGATTCATTCGTAGAAAGATTGTTAACTCCCCAACACATGCTGTGGGTGTCTTGTAGGTGTACCACGGATCTTCATCCATGAAGAAAGTGAAGAAATATCTTTTCTTGAAACACAGTTCTCAAATCAAAATGATATGCACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGGCAGGTTTTCTGATTGATTGGACCTGTTGTTATCTTTCAAGCTTCAAGATGGTCTTTGGATTGCTAGAAACTTTTGCCGGGATGATGGGGAAGGGAGCGGCTCTGTAAATTCATAG

mRNA sequence

ATGCCGAGTGTACCACGGATCTTCATCCATGAAGAAAGTGAAGAAATATCTTTTCTTGAAACACAGTTCTCAAATCAAAATGATATGCACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGGCAGCTTCAAGATGGTCTTTGGATTGCTAGAAACTTTTGCCGGGATGATGGGGAAGGGAGCGGCTCTGTAAATTCATAG

Coding sequence (CDS)

ATGCCGAGTGTACCACGGATCTTCATCCATGAAGAAAGTGAAGAAATATCTTTTCTTGAAACACAGTTCTCAAATCAAAATGATATGCACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGGCAGCTTCAAGATGGTCTTTGGATTGCTAGAAACTTTTGCCGGGATGATGGGGAAGGGAGCGGCTCTGTAAATTCATAG

Protein sequence

MPSVPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQLQDGLWIARNFCRDDGEGSGSVNS

Homology

[Close]

BLAST of Lsi04G006850 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 5.5e-06
Identity = 24/44 (54.55%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           +PRIF  EE+ +I  LE++ S+++   F EYI+TSRININIRQ+
Sbjct: 301 IPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIRQV 344

[Close]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-10
Identity = 37/44 (84.09%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLE   SNQ+D+HFLEYIRTSRININIRQ+
Sbjct: 319 VPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIRQV 362

[Close]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.8e-10
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLET  +NQ+D+H LEYIRTSRININIRQ+
Sbjct: 319 VPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQV 362

[Close]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-09
Identity = 34/44 (77.27%), Postives = 39/44 (88.64%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI  E++E S LE QFSNQ+D+HFLEYIRTSRININIRQ+
Sbjct: 318 VPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQV 361

[Close]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-09
Identity = 34/44 (77.27%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIF  EESEEIS LE +FS+++D+HFLEYIRTSRININIRQ+
Sbjct: 318 VPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQV 361

[Close]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 7.1e-09
Identity = 33/44 (75.00%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIF  EESEEIS LE +FS+++D+HFLEY+RTSRININIRQ+
Sbjct: 318 VPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQV 361

[Close]

BLAST of Lsi04G006850 vs. NCBI nr
Match: XP_008446031.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis melo])

HSP 1 Score: 75.5 bits (184), Expect = 2.1e-10
Identity = 37/44 (84.09%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLE   SNQ+D+HFLEYIRTSRININIRQ+
Sbjct: 319 VPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIRQV 362

[Close]

BLAST of Lsi04G006850 vs. NCBI nr
Match: XP_004135327.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus])

HSP 1 Score: 73.6 bits (179), Expect = 7.8e-10
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLET  +NQ+D+H LEYIRTSRININIRQ+
Sbjct: 319 VPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQV 362

[Close]

BLAST of Lsi04G006850 vs. NCBI nr
Match: XP_031740902.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X2 [Cucumis sativus])

HSP 1 Score: 73.6 bits (179), Expect = 7.8e-10
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLET  +NQ+D+H LEYIRTSRININIRQ+
Sbjct: 316 VPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQV 359

[Close]

BLAST of Lsi04G006850 vs. NCBI nr
Match: KAE8648677.1 (hypothetical protein Csa_009164 [Cucumis sativus])

HSP 1 Score: 73.6 bits (179), Expect = 7.8e-10
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEISFLET  +NQ+D+H LEYIRTSRININIRQ+
Sbjct: 211 VPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQV 254

[Close]

BLAST of Lsi04G006850 vs. NCBI nr
Match: XP_038891364.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida])

HSP 1 Score: 72.4 bits (176), Expect = 1.7e-09
Identity = 35/44 (79.55%), Postives = 39/44 (88.64%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           VPRIFI EESEEIS LE  FSNQ+D+HFLEYI+ SRININIRQ+
Sbjct: 319 VPRIFIDEESEEISLLERHFSNQDDLHFLEYIKNSRININIRQV 362

[Close]

BLAST of Lsi04G006850 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 51.2 bits (121), Expect = 3.9e-07
Identity = 24/44 (54.55%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 4   VPRIFIHEESEEISFLETQFSNQNDMHFLEYIRTSRININIRQL 48
           +PRIF  EE+ +I  LE++ S+++   F EYI+TSRININIRQ+
Sbjct: 301 IPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIRQV 344

The following BLAST results are available for this feature:

BLAST of Lsi04G006850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA98	5.5e-06	54.55	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Lsi04G006850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BE32	1.0e-10	84.09	LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m...	[more]
A0A0A0KS00	3.8e-10	81.82	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1DG90	3.2e-09	77.27	alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1FZQ9	3.2e-09	77.27	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQB4	7.1e-09	75.00	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Lsi04G006850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446031.1	2.1e-10	84.09	PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [...	[more]
XP_004135327.1	7.8e-10	81.82	alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus]	[more]
XP_031740902.1	7.8e-10	81.82	alpha-ketoglutarate-dependent dioxygenase alkB isoform X2 [Cucumis sativus]	[more]
KAE8648677.1	7.8e-10	81.82	hypothetical protein Csa_009164 [Cucumis sativus]	[more]
XP_038891364.1	1.7e-09	79.55	alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida]	[more]

BLAST of Lsi04G006850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11780.1	3.9e-07	54.55	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G006850.1	Lsi04G006850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0035513	oxidative RNA demethylation
biological_process	GO:0035552	oxidative single-stranded DNA demethylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0035516	oxidative DNA demethylase activity
molecular_function	GO:0035515	oxidative RNA demethylase activity