Lsi04G006500 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G006500
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionF-box protein SKIP14
Locationchr04: 5946555 .. 5949320 (+)
RNA-Seq ExpressionLsi04G006500
SyntenyLsi04G006500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTGAATTTTTCGCATCGACCGATTTTTCCCCCACATCTTACGGAGGAGAATTTGGTTTCTTCGATGAGTATTTCCAGTGGTTTTGCTGTCGATGGTGTTTCCGAGAGGAATTCTGATGTTTATGGGACGTCCTGGCTGATTAATCGGGAGCTCGTGGATTGCCTTAATTTTTTAGAGGATACCTGCGAAGTGGGTAGGTCGCGAGATTGTGTCCCTGGGGATGTTTTGGATCTCTTGCCTTCAGATCCATTTGGTATGGATATGAGTACCACTTTTACAGCGATCACAGGCTGGCTTGATGATCTGAATGTAGACTATGGTCGATATGGGAGAGCCCCGAATTACGAGCTTTTTGCTGAATTTAACTACATTTGTAACAGTGCCTTTAGGTTCCAGGCGTTTTCCCTTGGAAACGAAGGAATTCTTCATAGTACTGGTGAGCTGGGGGGATTCGGTGCGTGGTCAGATGAGAGAAAAGCAGATGGTGTGTCCTGCCATTCTGATCCTGAATCACCATATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAAGCACCATATCTTGTGGATACATTTCAGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAATTACCCTATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTTAGTTCGTGGTCTGATGGGATAAAAGCTGGTTTATCCTGCCATTCTGATCCTGAATCACCATATCTTTTGGATGCTTTTCGGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCTGGTGGTTTATCCTGCCATTCTGATCCCGAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCAGGTGGGTTATCCTGCCATTCTGATCCCAAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGGTGGGATAAAAGTAGGTGGTTTATCCTGCCATACTGATCCTCAATCATCATATGTTGTGGATACAATTCAGACTTTTGGTATTGAACCTGAAATTTCTGGAGGGGGATTCAATGCGTGGTCTGATGGAAGAAAAGCAGGTGCTGTATCCTTCCATACTGATCCTCGATCATCATATGTTGTGGATACAATTCAGACTCTTGTTATCGAACCTGCAATTTCAGGAGGGGAATTCGGTGTGTGGTCTGATGCGATAGAAGTAGGTGGTTTATCCTGCCTTGCTGAGCCTGAAGCACCACCATATGTTGTGGATACAATTAAGACTCTCGGTATTGAACCTGAAATTTCTGGAGGGAGATTCAGTGTGTGGTCTGATGGGAGAAAAACAGGTCCCTTTTCTTGCCATACTGAACCTCAATCATCATATGTTGTGGATACAATTAAGACTCTTTGTAATGAATCTGAAATTTCTGGAGGGGTATTCGTTGAATGGTCTGATGGGAGACAATCAGGTGCTGTATCCTGTTTTACTGATCCTGAATCACCACCATATTTTGTCGATACATGCCAGACTCTTGGTACTGAATCTGAAACTTCTGGGGTGCAACCTGTAGTTCTTCATGAGGGAGACTGTGTCTCTTTTGATGCACGTGGACCTCCTCATGCAGCTTTAAATTTTGTGCTTGGTTATCTTGGCACGCGGGAACTTCTTGTTGTTGAATCAGTTTGCAAGTCCCTGCAGTCAACTGCGGAAGGGGACCCTTTCTTTTGGAGAAACATTAACATATGTGGAAAGCTGGATGTGAAAATTACAGACGATGTTCTCCTAAATTTGACTAGCAAGGCTCAAGGTGGTCTGGAAAGTCTGAGCCTTGTAAACTGCGTAATGATAAGTGATGATGGTCTCAAGGAGGTGCTTCTCAATAATCCTAAGGTCACAAAGGTAGGGTAGTTTTGCACCGTTACTGTACAAAAGCTTCCTTTTCATTTTTTCTAAAAAAAAATATCAATTTTAAATAGTCGTTAGATTCTAATAAATTTTACTTCTTTTCTGTAGTTGTGTGTTCCAGGATGTACAAGACTCACCATTGGAGGCATTGTTGATAATTTGAAGGCCTTCAAGTCGAGAGGTGGACCGGGAATAAAACATTTAAGTATAGCTGGAATGTATGGAGTAACAGAAGTGCATTTTAAGGAGTTGGAGAAGCTGTTAGTAGGTATTGAGAACTTTACTCACCTAAATGCTCGCGAACCTCGATTTTATCGAGGAGGTGAACCTCGTTTTCCATCAAGCAATGGTGGGCGTGCCATTGACATAGAAAGATGCCCAAAATGTATGAATATGAGGATCGTTTATGACTGCCCGGTGGTTGGCTGCAAAGGAATAAAAGAAGGTGACACCAACCCTGATGCCAACACGCCGAGATGCAGGGCATGCACCCTTTGTATAGCTCGGTGCAATTGGTGTGGACGCTGCATCGATGAGACAGTACATGAGGAGACATTTTGTCTGGACTTGCGTTGCATTGATTGTGGGAAGGAGATATCGAAATGTGAATAA

mRNA sequence

ATGGCCTTGAATTTTTCGCATCGACCGATTTTTCCCCCACATCTTACGGAGGAGAATTTGGTTTCTTCGATGAGTATTTCCAGTGGTTTTGCTGTCGATGGTGTTTCCGAGAGGAATTCTGATGTTTATGGGACGTCCTGGCTGATTAATCGGGAGCTCGTGGATTGCCTTAATTTTTTAGAGGATACCTGCGAAGTGGGTAGGTCGCGAGATTGTGTCCCTGGGGATGTTTTGGATCTCTTGCCTTCAGATCCATTTGGTATGGATATGAGTACCACTTTTACAGCGATCACAGGCTGGCTTGATGATCTGAATGTAGACTATGGTCGATATGGGAGAGCCCCGAATTACGAGCTTTTTGCTGAATTTAACTACATTTGTAACAGTGCCTTTAGGTTCCAGGCGTTTTCCCTTGGAAACGAAGGAATTCTTCATAGTACTGGTGAGCTGGGGGGATTCGGTGCGTGGTCAGATGAGAGAAAAGCAGATGGTGTGTCCTGCCATTCTGATCCTGAATCACCATATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAAGCACCATATCTTGTGGATACATTTCAGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAATTACCCTATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTTAGTTCGTGGTCTGATGGGATAAAAGCTGGTTTATCCTGCCATTCTGATCCTGAATCACCATATCTTTTGGATGCTTTTCGGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCTGGTGGTTTATCCTGCCATTCTGATCCCGAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCAGGTGGGTTATCCTGCCATTCTGATCCCAAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGGTGGGATAAAAGTAGGTGGTTTATCCTGCCATACTGATCCTCAATCATCATATGTTGTGGATACAATTCAGACTTTTGGTATTGAACCTGAAATTTCTGGAGGGGGATTCAATGCGTGGTCTGATGGAAGAAAAGCAGGTGCTGTATCCTTCCATACTGATCCTCGATCATCATATGTTGTGGATACAATTCAGACTCTTGTTATCGAACCTGCAATTTCAGGAGGGGAATTCGGTGTGTGGTCTGATGCGATAGAAGTAGGTGGTTTATCCTGCCTTGCTGAGCCTGAAGCACCACCATATGTTGTGGATACAATTAAGACTCTCGGTATTGAACCTGAAATTTCTGGAGGGAGATTCAGTGTGTGGTCTGATGGGAGAAAAACAGGTCCCTTTTCTTGCCATACTGAACCTCAATCATCATATGTTGTGGATACAATTAAGACTCTTTGTAATGAATCTGAAATTTCTGGAGGGGTATTCGTTGAATGGTCTGATGGGAGACAATCAGGTGCTGTATCCTGTTTTACTGATCCTGAATCACCACCATATTTTGTCGATACATGCCAGACTCTTGGTACTGAATCTGAAACTTCTGGGGTGCAACCTGTAGTTCTTCATGAGGGAGACTGTGTCTCTTTTGATGCACGTGGACCTCCTCATGCAGCTTTAAATTTTGTGCTTGGTTATCTTGGCACGCGGGAACTTCTTGTTGTTGAATCAGTTTGCAAGTCCCTGCAGTCAACTGCGGAAGGGGACCCTTTCTTTTGGAGAAACATTAACATATGTGGAAAGCTGGATGTGAAAATTACAGACGATGTTCTCCTAAATTTGACTAGCAAGGCTCAAGGTGGTCTGGAAAGTCTGAGCCTTGTAAACTGCGTAATGATAAGTGATGATGGTCTCAAGGAGGTGCTTCTCAATAATCCTAAGGTCACAAAGTTGTGTGTTCCAGGATGTACAAGACTCACCATTGGAGGCATTGTTGATAATTTGAAGGCCTTCAAGTCGAGAGGTGGACCGGGAATAAAACATTTAAGTATAGCTGGAATGTATGGAGTAACAGAAGTGCATTTTAAGGAGTTGGAGAAGCTGTTAGTAGGTATTGAGAACTTTACTCACCTAAATGCTCGCGAACCTCGATTTTATCGAGGAGGTGAACCTCGTTTTCCATCAAGCAATGGTGGGCGTGCCATTGACATAGAAAGATGCCCAAAATGTATGAATATGAGGATCGTTTATGACTGCCCGGTGGTTGGCTGCAAAGGAATAAAAGAAGGTGACACCAACCCTGATGCCAACACGCCGAGATGCAGGGCATGCACCCTTTGTATAGCTCGGTGCAATTGGTGTGGACGCTGCATCGATGAGACAGTACATGAGGAGACATTTTGTCTGGACTTGCGTTGCATTGATTGTGGGAAGGAGATATCGAAATGTGAATAA

Coding sequence (CDS)

ATGGCCTTGAATTTTTCGCATCGACCGATTTTTCCCCCACATCTTACGGAGGAGAATTTGGTTTCTTCGATGAGTATTTCCAGTGGTTTTGCTGTCGATGGTGTTTCCGAGAGGAATTCTGATGTTTATGGGACGTCCTGGCTGATTAATCGGGAGCTCGTGGATTGCCTTAATTTTTTAGAGGATACCTGCGAAGTGGGTAGGTCGCGAGATTGTGTCCCTGGGGATGTTTTGGATCTCTTGCCTTCAGATCCATTTGGTATGGATATGAGTACCACTTTTACAGCGATCACAGGCTGGCTTGATGATCTGAATGTAGACTATGGTCGATATGGGAGAGCCCCGAATTACGAGCTTTTTGCTGAATTTAACTACATTTGTAACAGTGCCTTTAGGTTCCAGGCGTTTTCCCTTGGAAACGAAGGAATTCTTCATAGTACTGGTGAGCTGGGGGGATTCGGTGCGTGGTCAGATGAGAGAAAAGCAGATGGTGTGTCCTGCCATTCTGATCCTGAATCACCATATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAAGCACCATATCTTGTGGATACATTTCAGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCTGTTCGTGGTCTGATGGGATAAAAGCAGGTGGTTTATCCTGCCATTCTGATCCTGAATTACCCTATCTTGTCGATACAATTCAGACTCTTGGTATTGAACCTGAAGTTTCTGGAGATGGATTTAGTTCGTGGTCTGATGGGATAAAAGCTGGTTTATCCTGCCATTCTGATCCTGAATCACCATATCTTTTGGATGCTTTTCGGAATCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCTGGTGGTTTATCCTGCCATTCTGATCCCGAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGATGGGATAAAAGCAGGTGGGTTATCCTGCCATTCTGATCCCAAATCACCATATCTTGTGGATACATTTCAGACTCTTGGTATTGAACCTGAAATTTCTGGAGATGGATTCAGTTCGTGGTCTGGTGGGATAAAAGTAGGTGGTTTATCCTGCCATACTGATCCTCAATCATCATATGTTGTGGATACAATTCAGACTTTTGGTATTGAACCTGAAATTTCTGGAGGGGGATTCAATGCGTGGTCTGATGGAAGAAAAGCAGGTGCTGTATCCTTCCATACTGATCCTCGATCATCATATGTTGTGGATACAATTCAGACTCTTGTTATCGAACCTGCAATTTCAGGAGGGGAATTCGGTGTGTGGTCTGATGCGATAGAAGTAGGTGGTTTATCCTGCCTTGCTGAGCCTGAAGCACCACCATATGTTGTGGATACAATTAAGACTCTCGGTATTGAACCTGAAATTTCTGGAGGGAGATTCAGTGTGTGGTCTGATGGGAGAAAAACAGGTCCCTTTTCTTGCCATACTGAACCTCAATCATCATATGTTGTGGATACAATTAAGACTCTTTGTAATGAATCTGAAATTTCTGGAGGGGTATTCGTTGAATGGTCTGATGGGAGACAATCAGGTGCTGTATCCTGTTTTACTGATCCTGAATCACCACCATATTTTGTCGATACATGCCAGACTCTTGGTACTGAATCTGAAACTTCTGGGGTGCAACCTGTAGTTCTTCATGAGGGAGACTGTGTCTCTTTTGATGCACGTGGACCTCCTCATGCAGCTTTAAATTTTGTGCTTGGTTATCTTGGCACGCGGGAACTTCTTGTTGTTGAATCAGTTTGCAAGTCCCTGCAGTCAACTGCGGAAGGGGACCCTTTCTTTTGGAGAAACATTAACATATGTGGAAAGCTGGATGTGAAAATTACAGACGATGTTCTCCTAAATTTGACTAGCAAGGCTCAAGGTGGTCTGGAAAGTCTGAGCCTTGTAAACTGCGTAATGATAAGTGATGATGGTCTCAAGGAGGTGCTTCTCAATAATCCTAAGGTCACAAAGTTGTGTGTTCCAGGATGTACAAGACTCACCATTGGAGGCATTGTTGATAATTTGAAGGCCTTCAAGTCGAGAGGTGGACCGGGAATAAAACATTTAAGTATAGCTGGAATGTATGGAGTAACAGAAGTGCATTTTAAGGAGTTGGAGAAGCTGTTAGTAGGTATTGAGAACTTTACTCACCTAAATGCTCGCGAACCTCGATTTTATCGAGGAGGTGAACCTCGTTTTCCATCAAGCAATGGTGGGCGTGCCATTGACATAGAAAGATGCCCAAAATGTATGAATATGAGGATCGTTTATGACTGCCCGGTGGTTGGCTGCAAAGGAATAAAAGAAGGTGACACCAACCCTGATGCCAACACGCCGAGATGCAGGGCATGCACCCTTTGTATAGCTCGGTGCAATTGGTGTGGACGCTGCATCGATGAGACAGTACATGAGGAGACATTTTGTCTGGACTTGCGTTGCATTGATTGTGGGAAGGAGATATCGAAATGTGAATAA

Protein sequence

MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFLEDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGRYGRAPNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPESPYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGDGFCSWSDGIKAGGLSCHSDPELPYLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDPESPYLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPKSPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCHTDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEPAISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGPFSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQTLGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE
Homology
BLAST of Lsi04G006500 vs. ExPASy Swiss-Prot
Match: Q9LU91 (F-box protein SKIP14 OS=Arabidopsis thaliana OX=3702 GN=SKIP14 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 5.5e-63
Identity = 122/259 (47.10%), Postives = 165/259 (63.71%), Query Frame = 0

Query: 623 HAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLTS 682
           H A+ F L +L  ++LL V  VCKSL +T   D   W++I+IC  L+ KIT++ LL+LT 
Sbjct: 201 HPAIGFCLYHLRGKDLLSVSMVCKSLHTTVCDDTLLWKHIHICRPLNEKITEEALLHLTE 260

Query: 683 KAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRGG 742
           +AQG ++ L +V+C  I+DD LK V+  N +V K+ VPGCTR+TI GI+  L+  KS G 
Sbjct: 261 RAQGTMQCLRIVDCCRITDDCLKRVVARNRQVVKIGVPGCTRITIDGILSVLRDLKSAGK 320

Query: 743 PGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAID 802
             +KHL + G++GVT+ H+ EL  LL  I+N      ++PRFY  GE    S +  RA+D
Sbjct: 321 LQVKHLQLRGLFGVTKDHYDELIDLL-NIDNKVKQTIQKPRFYHRGE-ACVSCDDDRALD 380

Query: 803 IERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCIDETVH 862
           IE CPKC N ++VYDCP   CKG K+G       +  CRAC+LCI RC  CGRCI +T +
Sbjct: 381 IEMCPKCQNFKLVYDCPAEDCKGKKKG-------SEECRACSLCIQRCYHCGRCIIDTEY 440

Query: 863 EETFCLDLRCIDCGKEISK 882
           EE FCL+L C  C K   K
Sbjct: 441 EEMFCLELLCAVCSKPTPK 450


HSP 2 Score: 90.5 bits (223), Expect = 1.0e-16
Identity = 75/208 (36.06%), Postives = 97/208 (46.63%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRP F  HL+EE     M I++G                +W        C +F 
Sbjct: 1   MALNFSHRP-FSSHLSEE----PMMIANG----------------NW--------CSSF- 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDY-GRYGR------ 120
            D        D    D+LD+LPSDPFGMD++ TFTAITGWL+DL  DY  +YGR      
Sbjct: 61  -DNGRKNTGGDASSVDILDVLPSDPFGMDINNTFTAITGWLEDLEDDYNNQYGRRRRDDI 120

Query: 121 ----APNYELFAEFNYICNSAFRFQA--FSLGNEGIL---------------HSTGELGG 180
                   +LFA  ++  N+A +FQ+  +S G+E +                 S+GE  G
Sbjct: 121 WIGDGNRQQLFAGLSFFWNNAMQFQSSGYSYGSESLFGGAFDGSLFSTCKFPESSGENNG 173

BLAST of Lsi04G006500 vs. ExPASy Swiss-Prot
Match: Q9LK24 (F-box protein At3g27290 OS=Arabidopsis thaliana OX=3702 GN=At3g27290 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 1.3e-43
Identity = 102/259 (39.38%), Postives = 141/259 (54.44%), Query Frame = 0

Query: 620 GPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGK-LDVKITDDVLL 679
           G  H A   VL YL  +E+L VE VC+SL+ +   +PFFW +I++    L  ++TD+ LL
Sbjct: 125 GEAHDAFELVLPYLELKEILAVEVVCRSLRDSVGKEPFFWTSIDLNDSFLQYRVTDESLL 184

Query: 680 NLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFK 739
            LT +A GG+  L+L  CV I+D GLK+VL +NP +TKL V GC RL+  G+V  L+  K
Sbjct: 185 KLTRRALGGVRCLNLGGCVGITDYGLKQVLASNPHLTKLSVSGCLRLSTAGLVSTLRDLK 244

Query: 740 SRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGG 799
           S    G+K L   G    T+  FKEL  LL+G +    L  R+ RFY      F   +  
Sbjct: 245 SSNRLGVKSLITGGALYFTKEQFKEL-NLLLGGDAKVGLQERKKRFYTSCRSEFYLED-D 304

Query: 800 RAIDIERCPKCMNMRIVYDCPVVGC--KGIKEGDTNPDANTPRCRACTLCIARCNWCGRC 859
           R  D+E CP C    +V+DCP   C  KG          +   CRAC +CI RC+ CG C
Sbjct: 305 RVTDLEICPWCEKPSLVFDCPADTCPLKG------QYPYSKSSCRACVVCIERCHECGSC 364

Query: 860 IDETVHEETFCLDLRCIDC 876
           +++    + FC    C+ C
Sbjct: 365 LND-CENKPFCFAFSCVVC 374

BLAST of Lsi04G006500 vs. ExPASy Swiss-Prot
Match: Q9ZU90 (F-box protein SKIP28 OS=Arabidopsis thaliana OX=3702 GN=SKIP28 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 8.3e-27
Identity = 83/265 (31.32%), Postives = 130/265 (49.06%), Query Frame = 0

Query: 623 HAALNFVLGYLGTR-ELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLT 682
           H  L  VL YL +  ELL +  V +SL+     +   W  + I   L  ++TDD+L   +
Sbjct: 17  HEVLLIVLPYLHSLFELLSMIRVSRSLRDAIRDETALWTKLVIEPPLSSRLTDDILSEFS 76

Query: 683 SKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRG 742
           SK+ G L++L L  C+M+++ GL+ V+  NP +TK+ VPGC+ LT  GI++ +++  S+ 
Sbjct: 77  SKSAGKLKTLILRQCLMVTNKGLRRVVDANPLITKIIVPGCSGLTPEGIMECVESL-SKN 136

Query: 743 GPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAI 802
              ++ L I G+ G T+ H   L         +T+L                SS G   I
Sbjct: 137 NHKLETLHINGVNGFTKQHLSAL---------YTYL----------------SSEG--TI 196

Query: 803 DIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCI--DE 862
           D+E CPKC  +R++  C    C          +    +CR C LCI RC  C  C+   +
Sbjct: 197 DLEVCPKCDEVRMIPSCSRESC----------NQKQRKCRGCWLCIPRCAECAVCLVGSD 243

Query: 863 TVHEETFC--LDLRCIDCGKEISKC 883
           T  +E  C   D+ C++C   + KC
Sbjct: 257 TESQEAACGNDDVLCLECWLVLPKC 243

BLAST of Lsi04G006500 vs. ExPASy TrEMBL
Match: A0A0A0KQ13 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590200 PE=4 SV=1)

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 654/890 (73.48%), Postives = 685/890 (76.97%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRPIFP HLTEENLVSSM+ISSGF VD VSERN DVYGTSWLINRELVDCLNFL
Sbjct: 1   MALNFSHRPIFPSHLTEENLVSSMNISSGFVVDDVSERNFDVYGTSWLINRELVDCLNFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGR-YGR------ 120
           EDTCE G +RDCVPGDVLDLLPSDPF MD+STT TAITGWLDDLNVDYG  YGR      
Sbjct: 61  EDTCEGGGTRDCVPGDVLDLLPSDPFDMDISTTVTAITGWLDDLNVDYGSGYGRDERVPV 120

Query: 121 APNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPES 180
             NYELFA  NYI N+AFRFQAF LGN G  H TGELGGFGAWSDER             
Sbjct: 121 DENYELFAGLNYIWNNAFRFQAFPLGNGGNFHGTGELGGFGAWSDER------------- 180

Query: 181 PYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGD 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 GFCSWSDGIKAGGLSCHSDPELPYLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDP 300
                    KAG  SCHSD ELPYLVDTIQT                             
Sbjct: 241 ---------KAGSASCHSDSELPYLVDTIQT----------------------------- 300

Query: 301 ESPYLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEIS 360
                      LGIEPEISGDG SSWSDGIKAG LSCHSD ES YLVDTFQTLGIE EIS
Sbjct: 301 -----------LGIEPEISGDGLSSWSDGIKAGALSCHSDAESTYLVDTFQTLGIEREIS 360

Query: 361 GDGFSSWSDGIKAGGLSCHSDPKSPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCH 420
           GDGFSSWSDG K GGLS HSDP+S YLVDTFQTLGIEPE SGDGFSSWSGGIK GGLSCH
Sbjct: 361 GDGFSSWSDGTKGGGLSSHSDPESQYLVDTFQTLGIEPE-SGDGFSSWSGGIKAGGLSCH 420

Query: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEP 480
           T+PQSSYVVDTIQTFG+EPEISGGGF+AWSDGRKAGAVSFHTDPRSSY VDTIQTL IEP
Sbjct: 421 TEPQSSYVVDTIQTFGMEPEISGGGFSAWSDGRKAGAVSFHTDPRSSYAVDTIQTLDIEP 480

Query: 481 AISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGP 540
           AISGG F VWS  +E GG+SCLAEP++P YVVDTI+T G+EPE SG  FSVWSDGRKTG 
Sbjct: 481 AISGGGFSVWSGGMEAGGVSCLAEPDSPTYVVDTIQTFGVEPEFSGEGFSVWSDGRKTGG 540

Query: 541 FSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQT 600
            +CHT+PQSSYV+D+I T   ESE  GGVFV WSDGR+SG +S FT PESPPY VDTCQT
Sbjct: 541 LTCHTDPQSSYVMDSIHTHTIESEFPGGVFVTWSDGRKSGVMSYFTVPESPPYLVDTCQT 600

Query: 601 LGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660
           LGTE ETSGVQPVVLHE DCV  DA GPPHAA +FVLGYLGTRELL+VESVCK LQSTAE
Sbjct: 601 LGTEPETSGVQPVVLHEEDCVPIDASGPPHAAFSFVLGYLGTRELLLVESVCKFLQSTAE 660

Query: 661 GDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPK 720
           GDPFFWRNINICGK DVKITDDVLL LTSKAQGGLESLSLVNCVMISDDGL +VLLNNPK
Sbjct: 661 GDPFFWRNINICGKPDVKITDDVLLRLTSKAQGGLESLSLVNCVMISDDGLNKVLLNNPK 720

Query: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIEN 780
           VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLS+AG+YGVTEVH+KELEKLLVGIEN
Sbjct: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSVAGIYGVTEVHYKELEKLLVGIEN 767

Query: 781 FTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNP 840
            T L+  +PRFYRGGEPRFPSS+GGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDT+P
Sbjct: 781 LTQLDTCQPRFYRGGEPRFPSSDGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTDP 767

Query: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 884
           D N  RCRACT+CI RCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 DTNMARCRACTICIPRCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 767

BLAST of Lsi04G006500 vs. ExPASy TrEMBL
Match: A0A6J1FZJ0 (uncharacterized protein LOC111449320 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449320 PE=4 SV=1)

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 634/947 (66.95%), Postives = 711/947 (75.08%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFS+RPIFP HL+EEN VS MSIS GF VDG  ERNSDVYG SWLINREL +CL+FL
Sbjct: 1   MALNFSYRPIFPAHLSEENSVSPMSISGGFVVDGAPERNSDVYGMSWLINRELENCLDFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGRYGRA------ 120
           EDT E G SRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYG  G        
Sbjct: 61  EDTYEGGGSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGGGGGGGGGGSG 120

Query: 121 --------PNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVS 180
                    + +LFA  NYI N+AFR Q+F  GNEGI+HS+GE GGF  WSD R   G+S
Sbjct: 121 RDERVLVDESNQLFAGLNYIWNNAFRLQSFPHGNEGIVHSSGERGGFSEWSDGRNVGGLS 180

Query: 181 CHSDPESPYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGI 240
           CH+DP+S Y+VDTIQTLGIEPE+SG GF SWSD   AGGLSCH+D E+P+++DT Q LGI
Sbjct: 181 CHTDPQSSYVVDTIQTLGIEPEISGGGFGSWSDERNAGGLSCHTDLESPFVLDTIQTLGI 240

Query: 241 EPEISGDGFCSWSDGIKAGGLSCHSDPELP-YLVDTIQTLGIEPEVSGDGFSSWSDGIKA 300
           EPEISG GF +WSDG  AG +S  +D E P ++VDTIQ   IEPE+SG GF +WSDG   
Sbjct: 241 EPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQAFSIEPEISGGGFGAWSDGRTV 300

Query: 301 G-LSCHSDPESP-YLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESP-YLVDT 360
           G +S  +D ESP +++D  + LGIEPEISG GF +WSDG  A  +S  +D ESP ++VDT
Sbjct: 301 GCVSLRTDLESPRHVMDTIQTLGIEPEISGGGFGAWSDGRTADCVSFRTDLESPRHVVDT 360

Query: 361 FQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPKSP-YLVDTFQTLGIEPEISGDGFSSW 420
            QTLGIEPEISG GF +WSDG  AG +S  +D +SP ++VDT QTLGIEPEISG GFS+W
Sbjct: 361 IQTLGIEPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQTLGIEPEISGGGFSTW 420

Query: 421 SGGIKVGGLSCHTDPQS------------------------------------------- 480
           S G   G +S  TD +S                                           
Sbjct: 421 SDGRTAGCVSFCTDLESPRHVVDTIRTYSIDPEISGRGFGAWSDGRTAACVSLRTDLESP 480

Query: 481 SYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRS-SYVVDTIQTLVIEPAISG 540
            +VVDTIQT+ IEPEISGGGF AWSDGR AG VS  TD  S  +VVDTIQT  I+P ISG
Sbjct: 481 RHVVDTIQTYSIEPEISGGGFGAWSDGRTAGCVSLRTDLESPRHVVDTIQTYSIDPEISG 540

Query: 541 GEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGPFSCH 600
             FG WSD    G +S   + E+P +VVDTI+T  IEPEISGGRF  WSDGR  G  S  
Sbjct: 541 RGFGAWSDGRTAGCVSLRTDLESPRHVVDTIQTFSIEPEISGGRFGAWSDGRTAGCVSLR 600

Query: 601 TEPQS-SYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQTLGT 660
           T+ +S  +VVDTI+    E EISGG F  WSDGR++G VSC TDPESPPY +D  +TL  
Sbjct: 601 TDLESPRHVVDTIQNFSIEPEISGGGFGAWSDGRRAGGVSCHTDPESPPYSLDIIRTLSI 660

Query: 661 ESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDP 720
           E E S VQPVVL EG+CVS DA  PPHAALNFVLGYLGTRELLV+ESVCKSLQSTAE DP
Sbjct: 661 EPEISEVQPVVLEEGNCVSSDAGEPPHAALNFVLGYLGTRELLVIESVCKSLQSTAEDDP 720

Query: 721 FFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTK 780
           FFWRNINICGK+DVKITDD+LL LTSKAQGGLESLSLVNCVMISD+GL EVL NNPKVTK
Sbjct: 721 FFWRNINICGKVDVKITDDILLKLTSKAQGGLESLSLVNCVMISDNGLNEVLFNNPKVTK 780

Query: 781 LCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTH 840
           LCVPGCTRLTIGGIVD+LKAFK +G PG+KHLSIAGMYGVTE HFKELEKLL+G +N T 
Sbjct: 781 LCVPGCTRLTIGGIVDSLKAFKLKGRPGLKHLSIAGMYGVTEAHFKELEKLLLGTDNLTQ 840

Query: 841 LNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDAN 884
           LN  EPRFYRGG   FPS N GRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDT+P+AN
Sbjct: 841 LNTHEPRFYRGG-IHFPSCNDGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTDPNAN 900

BLAST of Lsi04G006500 vs. ExPASy TrEMBL
Match: A0A5D3CZT6 (F-box protein SKIP14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001980 PE=4 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 630/867 (72.66%), Postives = 659/867 (76.01%), Query Frame = 0

Query: 24  MSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFLEDTCEVGRSRDCVPGDVLDLLPS 83
           M+ISSGF VD VSERN DVYGTSWLINRELVDCLNFLEDTCE G SRD VPGDVLDLLPS
Sbjct: 1   MNISSGFVVDDVSERNFDVYGTSWLINRELVDCLNFLEDTCEGGGSRDRVPGDVLDLLPS 60

Query: 84  DPFGMDMSTTFTAITGWLDDLNVDYGR-YGR------APNYELFAEFNYICNSAFRFQAF 143
           DPFGMD+STT TAITGWLDDLNVDYG  YGR        NYELFA  NYI N+AFRFQ+F
Sbjct: 61  DPFGMDISTTVTAITGWLDDLNVDYGSGYGRDERVPVDENYELFAGLNYIWNNAFRFQSF 120

Query: 144 SLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPESPYLVDTIQTLGIEPEVSGDGFCS 203
            LGN GI H TGELGGFGAWSDER                                    
Sbjct: 121 PLGNGGIFHGTGELGGFGAWSDER------------------------------------ 180

Query: 204 WSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGDGFCSWSDGIKAGGLSCHSDPELP 263
                                                         KAG  SCHSD ELP
Sbjct: 181 ----------------------------------------------KAGNASCHSDSELP 240

Query: 264 YLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDPESPYLLDAFRNLGIEPEISGDGF 323
           YLVDTIQT                                        LGIEPEISGDGF
Sbjct: 241 YLVDTIQT----------------------------------------LGIEPEISGDGF 300

Query: 324 SSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPK 383
           SSWSDGIKAG LSCHSD ES YLVDTFQTLGIEPEISGDGFSSWSDG   G    HSDP+
Sbjct: 301 SSWSDGIKAGALSCHSDAESTYLVDTFQTLGIEPEISGDGFSSWSDGTTGG----HSDPE 360

Query: 384 SPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCHTDPQSSYVVDTIQTFGIEPEISG 443
           S Y VDTFQTLGIEPE SGDGFSSWSGGIK GGLSCHT+PQSSY VDTIQTFG+EPEISG
Sbjct: 361 SHYFVDTFQTLGIEPE-SGDGFSSWSGGIKAGGLSCHTEPQSSYAVDTIQTFGMEPEISG 420

Query: 444 GGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEPAISGGEFGVWSDAIEVGGLSCLA 503
           GGF+AWSDGRKAGAVSFHTDPRSSY VDTIQTL IEPAISGG F VWS  +E GGLSCLA
Sbjct: 421 GGFSAWSDGRKAGAVSFHTDPRSSYAVDTIQTLDIEPAISGGGFSVWSGGMEAGGLSCLA 480

Query: 504 EPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGPFSCHTEPQSSYVVDTIKTLCNES 563
           EPE+P YVVDTI+T G+EPE SGG FSVWSDGRKTG  SCHT+PQSSYV+D+I T   ES
Sbjct: 481 EPESPTYVVDTIQTFGVEPEFSGGGFSVWSDGRKTGGLSCHTDPQSSYVMDSIHTHTIES 540

Query: 564 EISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQTLGTESETSGVQPVVLHEGDCVSF 623
           E  GGVFV WSDGR+SG +S F  PESPPY V T QTLGTE ETS VQPVVLHEGD V F
Sbjct: 541 EFPGGVFVTWSDGRKSGVISYFPVPESPPYLVGTRQTLGTEPETSEVQPVVLHEGDSVPF 600

Query: 624 DARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDV 683
           DA GPPHAA +FVLGYLGTRELLVVESVCK LQSTAEGDPFFW+NINICGK DVKITDDV
Sbjct: 601 DASGPPHAAFSFVLGYLGTRELLVVESVCKFLQSTAEGDPFFWKNINICGKPDVKITDDV 660

Query: 684 LLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKA 743
           LL LTSKAQGGLESLSLVNCVMISDDGL +VLLNNPKVTKLCVPGCTRLTIGGIVDNLKA
Sbjct: 661 LLRLTSKAQGGLESLSLVNCVMISDDGLNKVLLNNPKVTKLCVPGCTRLTIGGIVDNLKA 720

Query: 744 FKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSN 803
           FKS GGPGIKHLS+AG+YGVTEVH+KELEKLLVGIEN THL+  +PRFYRGGEPRFPSS+
Sbjct: 721 FKSIGGPGIKHLSVAGIYGVTEVHYKELEKLLVGIENLTHLDTCQPRFYRGGEPRFPSSD 739

Query: 804 GGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRC 863
           GGRAIDIERCPKCMNMRIVYDCPVVGCKGIK GDT+P+ NTPRCRACT+CI RCNWCGRC
Sbjct: 781 GGRAIDIERCPKCMNMRIVYDCPVVGCKGIK-GDTDPNTNTPRCRACTICIPRCNWCGRC 739

Query: 864 IDETVHEETFCLDLRCIDCGKEISKCE 884
           IDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 IDETVHEETFCLDLRCIDCGKEISKCE 739

BLAST of Lsi04G006500 vs. ExPASy TrEMBL
Match: A0A6J1FZG4 (uncharacterized protein LOC111449320 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449320 PE=4 SV=1)

HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 610/904 (67.48%), Postives = 685/904 (75.77%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFS+RPIFP HL+EEN VS MSIS GF VDG  ERNSDVYG SWLINREL +CL+FL
Sbjct: 1   MALNFSYRPIFPAHLSEENSVSPMSISGGFVVDGAPERNSDVYGMSWLINRELENCLDFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGRYGRA------ 120
           EDT E G SRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYG  G        
Sbjct: 61  EDTYEGGGSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGGGGGGGGGGSG 120

Query: 121 --------PNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVS 180
                    + +LFA  NYI N+AFR Q+F  GNEGI+HS+GE GGF  WSD R   G+S
Sbjct: 121 RDERVLVDESNQLFAGLNYIWNNAFRLQSFPHGNEGIVHSSGERGGFSEWSDGRNVGGLS 180

Query: 181 CHSDPESPYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGI 240
           CH+DP+S Y+VDTIQTLGIEPE+SG GF SWSD   AGGLSCH+D E+P+++DT Q LGI
Sbjct: 181 CHTDPQSSYVVDTIQTLGIEPEISGGGFGSWSDERNAGGLSCHTDLESPFVLDTIQTLGI 240

Query: 241 EPEISGDGFCSWSDGIKAGGLSCHSDPELP-YLVDTIQTLGIEPEVSGDGFSSWSDGIKA 300
           EPEISG GF +WSDG  AG +S  +D E P ++VDTIQ   IEPE+SG GF +WSDG   
Sbjct: 241 EPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQAFSIEPEISGGGFGAWSDGRTV 300

Query: 301 G-LSCHSDPESP-YLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESP-YLVDT 360
           G +S  +D ESP +++D  + LGIEPEISG GF +WSDG  A  +S  +D ESP ++VDT
Sbjct: 301 GCVSLRTDLESPRHVMDTIQTLGIEPEISGGGFGAWSDGRTADCVSFRTDLESPRHVVDT 360

Query: 361 FQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPKSP-YLVDTFQTLGIEPEISGDGFSSW 420
            QTLGIEPEISG GF +WSDG  AG +S  +D +SP ++VDT QTLGIEPEISG GFS+W
Sbjct: 361 IQTLGIEPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQTLGIEPEISGGGFSTW 420

Query: 421 SGGIKVGGLSCHTDPQS-SYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSS 480
           S G   G +S  TD +S  +VVDTI+T+ I+PEISG GF AWSDGR A  VS  TD    
Sbjct: 421 SDGRTAGCVSFCTDLESPRHVVDTIRTYSIDPEISGRGFGAWSDGRTAACVSLRTD---- 480

Query: 481 YVVDTIQTLVIEPAISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGG 540
                                                 E+P +VVDTI+T  IEPEISGG
Sbjct: 481 -------------------------------------LESPRHVVDTIQTFSIEPEISGG 540

Query: 541 RFSVWSDGRKTGPFSCHTEPQS-SYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFT 600
           RF  WSDGR  G  S  T+ +S  +VVDTI+    E EISGG F  WSDGR++G VSC T
Sbjct: 541 RFGAWSDGRTAGCVSLRTDLESPRHVVDTIQNFSIEPEISGGGFGAWSDGRRAGGVSCHT 600

Query: 601 DPESPPYFVDTCQTLGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELL 660
           DPESPPY +D  +TL  E E S VQPVVL EG+CVS DA  PPHAALNFVLGYLGTRELL
Sbjct: 601 DPESPPYSLDIIRTLSIEPEISEVQPVVLEEGNCVSSDAGEPPHAALNFVLGYLGTRELL 660

Query: 661 VVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMI 720
           V+ESVCKSLQSTAE DPFFWRNINICGK+DVKITDD+LL LTSKAQGGLESLSLVNCVMI
Sbjct: 661 VIESVCKSLQSTAEDDPFFWRNINICGKVDVKITDDILLKLTSKAQGGLESLSLVNCVMI 720

Query: 721 SDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEV 780
           SD+GL EVL NNPKVTKLCVPGCTRLTIGGIVD+LKAFK +G PG+KHLSIAGMYGVTE 
Sbjct: 721 SDNGLNEVLFNNPKVTKLCVPGCTRLTIGGIVDSLKAFKLKGRPGLKHLSIAGMYGVTEA 780

Query: 781 HFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCP 840
           HFKELEKLL+G +N T LN  EPRFYRGG   FPS N GRAIDIERCPKCMNMRIVYDCP
Sbjct: 781 HFKELEKLLLGTDNLTQLNTHEPRFYRGG-IHFPSCNDGRAIDIERCPKCMNMRIVYDCP 840

Query: 841 VVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEI 884
           VVGCKGIKEGDT+P+AN  RCR CTLCIARCNWCGRCIDETVHEETF LDLRCIDCGK+I
Sbjct: 841 VVGCKGIKEGDTDPNANAQRCRGCTLCIARCNWCGRCIDETVHEETFSLDLRCIDCGKKI 862

BLAST of Lsi04G006500 vs. ExPASy TrEMBL
Match: A0A6J1FZG1 (F-box protein SKIP14-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449320 PE=4 SV=1)

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 587/903 (65.01%), Postives = 657/903 (72.76%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFS+RPIFP HL+EEN VS MSIS GF VDG  ERNSDVYG SWLINREL +CL+FL
Sbjct: 1   MALNFSYRPIFPAHLSEENSVSPMSISGGFVVDGAPERNSDVYGMSWLINRELENCLDFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGRYGRA------ 120
           EDT E G SRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYG  G        
Sbjct: 61  EDTYEGGGSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGGGGGGGGGGSG 120

Query: 121 --------PNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVS 180
                    + +LFA  NYI N+AFR Q+F  GNEGI+HS+GE GGF  WSD R   G+S
Sbjct: 121 RDERVLVDESNQLFAGLNYIWNNAFRLQSFPHGNEGIVHSSGERGGFSEWSDGRNVGGLS 180

Query: 181 CHSDPESPYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGI 240
           CH+DP+S Y+VDTIQTLGIEPE+SG GF SWSD   AGGLSCH+D E+P+++DT Q LGI
Sbjct: 181 CHTDPQSSYVVDTIQTLGIEPEISGGGFGSWSDERNAGGLSCHTDLESPFVLDTIQTLGI 240

Query: 241 EPEISGDGFCSWSDGIKAGGLSCHSDPELP-YLVDTIQTLGIEPEVSGDGFSSWSDGIKA 300
           EPEISG GF +WSDG  AG +S  +D E P ++VDTIQ   IEPE+SG GF +WSDG   
Sbjct: 241 EPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQAFSIEPEISGGGFGAWSDGRTV 300

Query: 301 G-LSCHSDPESP-YLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESP-YLVDT 360
           G +S  +D ESP +++D  + LGIEPEISG GF +WSDG  A  +S  +D ESP ++VDT
Sbjct: 301 GCVSLRTDLESPRHVMDTIQTLGIEPEISGGGFGAWSDGRTADCVSFRTDLESPRHVVDT 360

Query: 361 FQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPKSP-YLVDTFQTLGIEPEISGDGFSSW 420
            QTLGIEPEISG GF +WSDG  AG +S  +D +SP ++VDT QTLGIEPEISG GFS+W
Sbjct: 361 IQTLGIEPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQTLGIEPEISGGGFSTW 420

Query: 421 SGGIKVGGLSCHTDPQS-SYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSS 480
           S G   G +S  TD +S  +VVDTI+T+ I+PEISG GF AWSDGR A  VS  TD    
Sbjct: 421 SDGRTAGCVSFCTDLESPRHVVDTIRTYSIDPEISGRGFGAWSDGRTAACVSLRTD---- 480

Query: 481 YVVDTIQTLVIEPAISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGG 540
                                                 E+P +VVDTI+   IEPEISGG
Sbjct: 481 -------------------------------------LESPRHVVDTIQNFSIEPEISGG 540

Query: 541 RFSVWSDGRKTGPFSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTD 600
            F  WSDGR+ G  SCH                                         TD
Sbjct: 541 GFGAWSDGRRAGGVSCH-----------------------------------------TD 600

Query: 601 PESPPYFVDTCQTLGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLV 660
           PESPPY +D  +TL  E E S VQPVVL EG+CVS DA  PPHAALNFVLGYLGTRELLV
Sbjct: 601 PESPPYSLDIIRTLSIEPEISEVQPVVLEEGNCVSSDAGEPPHAALNFVLGYLGTRELLV 660

Query: 661 VESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMIS 720
           +ESVCKSLQSTAE DPFFWRNINICGK+DVKITDD+LL LTSKAQGGLESLSLVNCVMIS
Sbjct: 661 IESVCKSLQSTAEDDPFFWRNINICGKVDVKITDDILLKLTSKAQGGLESLSLVNCVMIS 720

Query: 721 DDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVH 780
           D+GL EVL NNPKVTKLCVPGCTRLTIGGIVD+LKAFK +G PG+KHLSIAGMYGVTE H
Sbjct: 721 DNGLNEVLFNNPKVTKLCVPGCTRLTIGGIVDSLKAFKLKGRPGLKHLSIAGMYGVTEAH 780

Query: 781 FKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPV 840
           FKELEKLL+G +N T LN  EPRFYRGG   FPS N GRAIDIERCPKCMNMRIVYDCPV
Sbjct: 781 FKELEKLLLGTDNLTQLNTHEPRFYRGG-IHFPSCNDGRAIDIERCPKCMNMRIVYDCPV 820

Query: 841 VGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEIS 884
           VGCKGIKEGDT+P+AN  RCR CTLCIARCNWCGRCIDETVHEETF LDLRCIDCGK+IS
Sbjct: 841 VGCKGIKEGDTDPNANAQRCRGCTLCIARCNWCGRCIDETVHEETFSLDLRCIDCGKKIS 820

BLAST of Lsi04G006500 vs. NCBI nr
Match: XP_038892073.1 (uncharacterized protein LOC120081355 isoform X1 [Benincasa hispida])

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 680/890 (76.40%), Postives = 699/890 (78.54%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRPIFPP+LTEENLVSS++IS GF VDGVSERNSDVYGTSWLINRELVDCLNFL
Sbjct: 1   MALNFSHRPIFPPYLTEENLVSSVNISRGFVVDGVSERNSDVYGTSWLINRELVDCLNFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDY-GRYGRAPN--- 120
           EDTC+ G SRD V GDVLDLLPSDPFGMD+STTFTAITG LDDLNVDY G YGR      
Sbjct: 61  EDTCDGGGSRDRVRGDVLDLLPSDPFGMDISTTFTAITGLLDDLNVDYGGGYGRDERVPV 120

Query: 121 ---YELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPES 180
              YELFA  NYI  +AFRFQAF L NEGIL STG+LGGF  WSDER             
Sbjct: 121 DGCYELFAGLNYIWTNAFRFQAFPLRNEGILPSTGQLGGFDVWSDER------------- 180

Query: 181 PYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGD 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 GFCSWSDGIKAGGLSCHSDPELPYLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDP 300
                    KAGG SCHSD ELPYLVD IQT                             
Sbjct: 241 ---------KAGGASCHSDLELPYLVDIIQT----------------------------- 300

Query: 301 ESPYLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEIS 360
                      LGIEPE SGDGFSSWSDG+KAGGLSCHSDPES Y VDTFQ+LGIE +IS
Sbjct: 301 -----------LGIEPEFSGDGFSSWSDGMKAGGLSCHSDPESRYGVDTFQSLGIERKIS 360

Query: 361 GDGFSSWSDGIKAGGLSCHSDPKSPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCH 420
           GDGFSSWSDGIK GGLSCHSDP+SPYLVDTFQTLGIEPEISGDGFSSWSGGIK GGLSCH
Sbjct: 361 GDGFSSWSDGIKVGGLSCHSDPESPYLVDTFQTLGIEPEISGDGFSSWSGGIKAGGLSCH 420

Query: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEP 480
           TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDP+SSY VDTIQTLV EP
Sbjct: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPQSSYAVDTIQTLVTEP 480

Query: 481 AISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGP 540
           AISGG FG WSD IEVGGLSCL EPE PPYVVDT +TLGIEPEISGG FSVWS  RKTGP
Sbjct: 481 AISGGRFGAWSDGIEVGGLSCLVEPETPPYVVDTFQTLGIEPEISGGGFSVWSHRRKTGP 540

Query: 541 FSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQT 600
           FSCHT+PQSSYVV+ IKT   ESEI GGVFVEWSDGRQSGAVSCFTDPESPPYFVDT QT
Sbjct: 541 FSCHTDPQSSYVVEAIKTFTIESEIPGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTYQT 600

Query: 601 LGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660
           LGTE ETSG+QPVV HE D VS D  GPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE
Sbjct: 601 LGTEPETSGLQPVVPHEEDRVSSDESGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660

Query: 661 GDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPK 720
           GDPFFW+NINICGKLDVKITDDVLL LTSKAQGGLESLSLVNCVMISDDGL EVLL NPK
Sbjct: 661 GDPFFWKNINICGKLDVKITDDVLLKLTSKAQGGLESLSLVNCVMISDDGLNEVLLKNPK 720

Query: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIEN 780
           VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVH+KELEKLLVGIEN
Sbjct: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHYKELEKLLVGIEN 768

Query: 781 FTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNP 840
            TH N  +PRFYRGGEPRFPSSNGGR IDIERCPKCMNMRIVYDCPVVGCKGIKEGDTN 
Sbjct: 781 LTHPNTPQPRFYRGGEPRFPSSNGGRVIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNT 768

Query: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 884
           DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 768

BLAST of Lsi04G006500 vs. NCBI nr
Match: XP_004135509.1 (F-box protein SKIP14 [Cucumis sativus] >KGN51703.1 hypothetical protein Csa_009019 [Cucumis sativus])

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 654/890 (73.48%), Postives = 685/890 (76.97%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRPIFP HLTEENLVSSM+ISSGF VD VSERN DVYGTSWLINRELVDCLNFL
Sbjct: 1   MALNFSHRPIFPSHLTEENLVSSMNISSGFVVDDVSERNFDVYGTSWLINRELVDCLNFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGR-YGR------ 120
           EDTCE G +RDCVPGDVLDLLPSDPF MD+STT TAITGWLDDLNVDYG  YGR      
Sbjct: 61  EDTCEGGGTRDCVPGDVLDLLPSDPFDMDISTTVTAITGWLDDLNVDYGSGYGRDERVPV 120

Query: 121 APNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPES 180
             NYELFA  NYI N+AFRFQAF LGN G  H TGELGGFGAWSDER             
Sbjct: 121 DENYELFAGLNYIWNNAFRFQAFPLGNGGNFHGTGELGGFGAWSDER------------- 180

Query: 181 PYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGD 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 GFCSWSDGIKAGGLSCHSDPELPYLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDP 300
                    KAG  SCHSD ELPYLVDTIQT                             
Sbjct: 241 ---------KAGSASCHSDSELPYLVDTIQT----------------------------- 300

Query: 301 ESPYLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEIS 360
                      LGIEPEISGDG SSWSDGIKAG LSCHSD ES YLVDTFQTLGIE EIS
Sbjct: 301 -----------LGIEPEISGDGLSSWSDGIKAGALSCHSDAESTYLVDTFQTLGIEREIS 360

Query: 361 GDGFSSWSDGIKAGGLSCHSDPKSPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCH 420
           GDGFSSWSDG K GGLS HSDP+S YLVDTFQTLGIEPE SGDGFSSWSGGIK GGLSCH
Sbjct: 361 GDGFSSWSDGTKGGGLSSHSDPESQYLVDTFQTLGIEPE-SGDGFSSWSGGIKAGGLSCH 420

Query: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEP 480
           T+PQSSYVVDTIQTFG+EPEISGGGF+AWSDGRKAGAVSFHTDPRSSY VDTIQTL IEP
Sbjct: 421 TEPQSSYVVDTIQTFGMEPEISGGGFSAWSDGRKAGAVSFHTDPRSSYAVDTIQTLDIEP 480

Query: 481 AISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGP 540
           AISGG F VWS  +E GG+SCLAEP++P YVVDTI+T G+EPE SG  FSVWSDGRKTG 
Sbjct: 481 AISGGGFSVWSGGMEAGGVSCLAEPDSPTYVVDTIQTFGVEPEFSGEGFSVWSDGRKTGG 540

Query: 541 FSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQT 600
            +CHT+PQSSYV+D+I T   ESE  GGVFV WSDGR+SG +S FT PESPPY VDTCQT
Sbjct: 541 LTCHTDPQSSYVMDSIHTHTIESEFPGGVFVTWSDGRKSGVMSYFTVPESPPYLVDTCQT 600

Query: 601 LGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660
           LGTE ETSGVQPVVLHE DCV  DA GPPHAA +FVLGYLGTRELL+VESVCK LQSTAE
Sbjct: 601 LGTEPETSGVQPVVLHEEDCVPIDASGPPHAAFSFVLGYLGTRELLLVESVCKFLQSTAE 660

Query: 661 GDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPK 720
           GDPFFWRNINICGK DVKITDDVLL LTSKAQGGLESLSLVNCVMISDDGL +VLLNNPK
Sbjct: 661 GDPFFWRNINICGKPDVKITDDVLLRLTSKAQGGLESLSLVNCVMISDDGLNKVLLNNPK 720

Query: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIEN 780
           VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLS+AG+YGVTEVH+KELEKLLVGIEN
Sbjct: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSVAGIYGVTEVHYKELEKLLVGIEN 767

Query: 781 FTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNP 840
            T L+  +PRFYRGGEPRFPSS+GGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDT+P
Sbjct: 781 LTQLDTCQPRFYRGGEPRFPSSDGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTDP 767

Query: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 884
           D N  RCRACT+CI RCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 DTNMARCRACTICIPRCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 767

BLAST of Lsi04G006500 vs. NCBI nr
Match: XP_038892074.1 (uncharacterized protein LOC120081355 isoform X2 [Benincasa hispida])

HSP 1 Score: 1296.2 bits (3353), Expect = 0.0e+00
Identity = 656/890 (73.71%), Postives = 675/890 (75.84%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRPIFPP+LTEENLVSS++IS GF VDGVSERNSDVYGTSWLINRELVDCLNFL
Sbjct: 1   MALNFSHRPIFPPYLTEENLVSSVNISRGFVVDGVSERNSDVYGTSWLINRELVDCLNFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDY-GRYGRAPN--- 120
           EDTC+ G SRD V GDVLDLLPSDPFGMD+STTFTAITG LDDLNVDY G YGR      
Sbjct: 61  EDTCDGGGSRDRVRGDVLDLLPSDPFGMDISTTFTAITGLLDDLNVDYGGGYGRDERVPV 120

Query: 121 ---YELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPES 180
              YELFA  NYI  +AFRFQAF L NEGIL STG+LGGF  WSDERKA           
Sbjct: 121 DGCYELFAGLNYIWTNAFRFQAFPLRNEGILPSTGQLGGFDVWSDERKA----------- 180

Query: 181 PYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGD 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 GFCSWSDGIKAGGLSCHSDPELPYLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDP 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 301 ESPYLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEIS 360
                              GDGFSSWSDG+KAGGLSCHSDPES Y VDTFQ+LGIE +IS
Sbjct: 301 -------------------GDGFSSWSDGMKAGGLSCHSDPESRYGVDTFQSLGIERKIS 360

Query: 361 GDGFSSWSDGIKAGGLSCHSDPKSPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCH 420
           GDGFSSWSDGIK GGLSCHSDP+SPYLVDTFQTLGIEPEISGDGFSSWSGGIK GGLSCH
Sbjct: 361 GDGFSSWSDGIKVGGLSCHSDPESPYLVDTFQTLGIEPEISGDGFSSWSGGIKAGGLSCH 420

Query: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEP 480
           TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDP+SSY VDTIQTLV EP
Sbjct: 421 TDPQSSYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPQSSYAVDTIQTLVTEP 480

Query: 481 AISGGEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGP 540
           AISGG FG WSD IEVGGLSCL EPE PPYVVDT +TLGIEPEISGG FSVWS  RKTGP
Sbjct: 481 AISGGRFGAWSDGIEVGGLSCLVEPETPPYVVDTFQTLGIEPEISGGGFSVWSHRRKTGP 540

Query: 541 FSCHTEPQSSYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQT 600
           FSCHT+PQSSYVV+ IKT   ESEI GGVFVEWSDGRQSGAVSCFTDPESPPYFVDT QT
Sbjct: 541 FSCHTDPQSSYVVEAIKTFTIESEIPGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTYQT 600

Query: 601 LGTESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660
           LGTE ETSG+QPVV HE D VS D  GPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE
Sbjct: 601 LGTEPETSGLQPVVPHEEDRVSSDESGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAE 660

Query: 661 GDPFFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPK 720
           GDPFFW+NINICGKLDVKITDDVLL LTSKAQGGLESLSLVNCVMISDDGL EVLL NPK
Sbjct: 661 GDPFFWKNINICGKLDVKITDDVLLKLTSKAQGGLESLSLVNCVMISDDGLNEVLLKNPK 720

Query: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIEN 780
           VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVH+KELEKLLVGIEN
Sbjct: 721 VTKLCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHYKELEKLLVGIEN 740

Query: 781 FTHLNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNP 840
            TH N  +PRFYRGGEPRFPSSNGGR IDIERCPKCMNMRIVYDCPVVGCKGIKEGDTN 
Sbjct: 781 LTHPNTPQPRFYRGGEPRFPSSNGGRVIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNT 740

Query: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 884
           DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 DANTPRCRACTLCIARCNWCGRCIDETVHEETFCLDLRCIDCGKEISKCE 740

BLAST of Lsi04G006500 vs. NCBI nr
Match: XP_022944934.1 (uncharacterized protein LOC111449320 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 634/947 (66.95%), Postives = 711/947 (75.08%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFS+RPIFP HL+EEN VS MSIS GF VDG  ERNSDVYG SWLINREL +CL+FL
Sbjct: 1   MALNFSYRPIFPAHLSEENSVSPMSISGGFVVDGAPERNSDVYGMSWLINRELENCLDFL 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGRYGRA------ 120
           EDT E G SRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYG  G        
Sbjct: 61  EDTYEGGGSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDYGGGGGGGGGGSG 120

Query: 121 --------PNYELFAEFNYICNSAFRFQAFSLGNEGILHSTGELGGFGAWSDERKADGVS 180
                    + +LFA  NYI N+AFR Q+F  GNEGI+HS+GE GGF  WSD R   G+S
Sbjct: 121 RDERVLVDESNQLFAGLNYIWNNAFRLQSFPHGNEGIVHSSGERGGFSEWSDGRNVGGLS 180

Query: 181 CHSDPESPYLVDTIQTLGIEPEVSGDGFCSWSDGIKAGGLSCHSDPEAPYLVDTFQNLGI 240
           CH+DP+S Y+VDTIQTLGIEPE+SG GF SWSD   AGGLSCH+D E+P+++DT Q LGI
Sbjct: 181 CHTDPQSSYVVDTIQTLGIEPEISGGGFGSWSDERNAGGLSCHTDLESPFVLDTIQTLGI 240

Query: 241 EPEISGDGFCSWSDGIKAGGLSCHSDPELP-YLVDTIQTLGIEPEVSGDGFSSWSDGIKA 300
           EPEISG GF +WSDG  AG +S  +D E P ++VDTIQ   IEPE+SG GF +WSDG   
Sbjct: 241 EPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQAFSIEPEISGGGFGAWSDGRTV 300

Query: 301 G-LSCHSDPESP-YLLDAFRNLGIEPEISGDGFSSWSDGIKAGGLSCHSDPESP-YLVDT 360
           G +S  +D ESP +++D  + LGIEPEISG GF +WSDG  A  +S  +D ESP ++VDT
Sbjct: 301 GCVSLRTDLESPRHVMDTIQTLGIEPEISGGGFGAWSDGRTADCVSFRTDLESPRHVVDT 360

Query: 361 FQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPKSP-YLVDTFQTLGIEPEISGDGFSSW 420
            QTLGIEPEISG GF +WSDG  AG +S  +D +SP ++VDT QTLGIEPEISG GFS+W
Sbjct: 361 IQTLGIEPEISGGGFGAWSDGRTAGCVSFRTDLESPRHVVDTIQTLGIEPEISGGGFSTW 420

Query: 421 SGGIKVGGLSCHTDPQS------------------------------------------- 480
           S G   G +S  TD +S                                           
Sbjct: 421 SDGRTAGCVSFCTDLESPRHVVDTIRTYSIDPEISGRGFGAWSDGRTAACVSLRTDLESP 480

Query: 481 SYVVDTIQTFGIEPEISGGGFNAWSDGRKAGAVSFHTDPRS-SYVVDTIQTLVIEPAISG 540
            +VVDTIQT+ IEPEISGGGF AWSDGR AG VS  TD  S  +VVDTIQT  I+P ISG
Sbjct: 481 RHVVDTIQTYSIEPEISGGGFGAWSDGRTAGCVSLRTDLESPRHVVDTIQTYSIDPEISG 540

Query: 541 GEFGVWSDAIEVGGLSCLAEPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGPFSCH 600
             FG WSD    G +S   + E+P +VVDTI+T  IEPEISGGRF  WSDGR  G  S  
Sbjct: 541 RGFGAWSDGRTAGCVSLRTDLESPRHVVDTIQTFSIEPEISGGRFGAWSDGRTAGCVSLR 600

Query: 601 TEPQS-SYVVDTIKTLCNESEISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQTLGT 660
           T+ +S  +VVDTI+    E EISGG F  WSDGR++G VSC TDPESPPY +D  +TL  
Sbjct: 601 TDLESPRHVVDTIQNFSIEPEISGGGFGAWSDGRRAGGVSCHTDPESPPYSLDIIRTLSI 660

Query: 661 ESETSGVQPVVLHEGDCVSFDARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDP 720
           E E S VQPVVL EG+CVS DA  PPHAALNFVLGYLGTRELLV+ESVCKSLQSTAE DP
Sbjct: 661 EPEISEVQPVVLEEGNCVSSDAGEPPHAALNFVLGYLGTRELLVIESVCKSLQSTAEDDP 720

Query: 721 FFWRNINICGKLDVKITDDVLLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTK 780
           FFWRNINICGK+DVKITDD+LL LTSKAQGGLESLSLVNCVMISD+GL EVL NNPKVTK
Sbjct: 721 FFWRNINICGKVDVKITDDILLKLTSKAQGGLESLSLVNCVMISDNGLNEVLFNNPKVTK 780

Query: 781 LCVPGCTRLTIGGIVDNLKAFKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTH 840
           LCVPGCTRLTIGGIVD+LKAFK +G PG+KHLSIAGMYGVTE HFKELEKLL+G +N T 
Sbjct: 781 LCVPGCTRLTIGGIVDSLKAFKLKGRPGLKHLSIAGMYGVTEAHFKELEKLLLGTDNLTQ 840

Query: 841 LNAREPRFYRGGEPRFPSSNGGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDAN 884
           LN  EPRFYRGG   FPS N GRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDT+P+AN
Sbjct: 841 LNTHEPRFYRGG-IHFPSCNDGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTDPNAN 900

BLAST of Lsi04G006500 vs. NCBI nr
Match: KAA0034221.1 (F-box protein SKIP14 [Cucumis melo var. makuwa] >TYK15699.1 F-box protein SKIP14 [Cucumis melo var. makuwa])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 630/867 (72.66%), Postives = 659/867 (76.01%), Query Frame = 0

Query: 24  MSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFLEDTCEVGRSRDCVPGDVLDLLPS 83
           M+ISSGF VD VSERN DVYGTSWLINRELVDCLNFLEDTCE G SRD VPGDVLDLLPS
Sbjct: 1   MNISSGFVVDDVSERNFDVYGTSWLINRELVDCLNFLEDTCEGGGSRDRVPGDVLDLLPS 60

Query: 84  DPFGMDMSTTFTAITGWLDDLNVDYGR-YGR------APNYELFAEFNYICNSAFRFQAF 143
           DPFGMD+STT TAITGWLDDLNVDYG  YGR        NYELFA  NYI N+AFRFQ+F
Sbjct: 61  DPFGMDISTTVTAITGWLDDLNVDYGSGYGRDERVPVDENYELFAGLNYIWNNAFRFQSF 120

Query: 144 SLGNEGILHSTGELGGFGAWSDERKADGVSCHSDPESPYLVDTIQTLGIEPEVSGDGFCS 203
            LGN GI H TGELGGFGAWSDER                                    
Sbjct: 121 PLGNGGIFHGTGELGGFGAWSDER------------------------------------ 180

Query: 204 WSDGIKAGGLSCHSDPEAPYLVDTFQNLGIEPEISGDGFCSWSDGIKAGGLSCHSDPELP 263
                                                         KAG  SCHSD ELP
Sbjct: 181 ----------------------------------------------KAGNASCHSDSELP 240

Query: 264 YLVDTIQTLGIEPEVSGDGFSSWSDGIKAGLSCHSDPESPYLLDAFRNLGIEPEISGDGF 323
           YLVDTIQT                                        LGIEPEISGDGF
Sbjct: 241 YLVDTIQT----------------------------------------LGIEPEISGDGF 300

Query: 324 SSWSDGIKAGGLSCHSDPESPYLVDTFQTLGIEPEISGDGFSSWSDGIKAGGLSCHSDPK 383
           SSWSDGIKAG LSCHSD ES YLVDTFQTLGIEPEISGDGFSSWSDG   G    HSDP+
Sbjct: 301 SSWSDGIKAGALSCHSDAESTYLVDTFQTLGIEPEISGDGFSSWSDGTTGG----HSDPE 360

Query: 384 SPYLVDTFQTLGIEPEISGDGFSSWSGGIKVGGLSCHTDPQSSYVVDTIQTFGIEPEISG 443
           S Y VDTFQTLGIEPE SGDGFSSWSGGIK GGLSCHT+PQSSY VDTIQTFG+EPEISG
Sbjct: 361 SHYFVDTFQTLGIEPE-SGDGFSSWSGGIKAGGLSCHTEPQSSYAVDTIQTFGMEPEISG 420

Query: 444 GGFNAWSDGRKAGAVSFHTDPRSSYVVDTIQTLVIEPAISGGEFGVWSDAIEVGGLSCLA 503
           GGF+AWSDGRKAGAVSFHTDPRSSY VDTIQTL IEPAISGG F VWS  +E GGLSCLA
Sbjct: 421 GGFSAWSDGRKAGAVSFHTDPRSSYAVDTIQTLDIEPAISGGGFSVWSGGMEAGGLSCLA 480

Query: 504 EPEAPPYVVDTIKTLGIEPEISGGRFSVWSDGRKTGPFSCHTEPQSSYVVDTIKTLCNES 563
           EPE+P YVVDTI+T G+EPE SGG FSVWSDGRKTG  SCHT+PQSSYV+D+I T   ES
Sbjct: 481 EPESPTYVVDTIQTFGVEPEFSGGGFSVWSDGRKTGGLSCHTDPQSSYVMDSIHTHTIES 540

Query: 564 EISGGVFVEWSDGRQSGAVSCFTDPESPPYFVDTCQTLGTESETSGVQPVVLHEGDCVSF 623
           E  GGVFV WSDGR+SG +S F  PESPPY V T QTLGTE ETS VQPVVLHEGD V F
Sbjct: 541 EFPGGVFVTWSDGRKSGVISYFPVPESPPYLVGTRQTLGTEPETSEVQPVVLHEGDSVPF 600

Query: 624 DARGPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDV 683
           DA GPPHAA +FVLGYLGTRELLVVESVCK LQSTAEGDPFFW+NINICGK DVKITDDV
Sbjct: 601 DASGPPHAAFSFVLGYLGTRELLVVESVCKFLQSTAEGDPFFWKNINICGKPDVKITDDV 660

Query: 684 LLNLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKA 743
           LL LTSKAQGGLESLSLVNCVMISDDGL +VLLNNPKVTKLCVPGCTRLTIGGIVDNLKA
Sbjct: 661 LLRLTSKAQGGLESLSLVNCVMISDDGLNKVLLNNPKVTKLCVPGCTRLTIGGIVDNLKA 720

Query: 744 FKSRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSN 803
           FKS GGPGIKHLS+AG+YGVTEVH+KELEKLLVGIEN THL+  +PRFYRGGEPRFPSS+
Sbjct: 721 FKSIGGPGIKHLSVAGIYGVTEVHYKELEKLLVGIENLTHLDTCQPRFYRGGEPRFPSSD 739

Query: 804 GGRAIDIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRC 863
           GGRAIDIERCPKCMNMRIVYDCPVVGCKGIK GDT+P+ NTPRCRACT+CI RCNWCGRC
Sbjct: 781 GGRAIDIERCPKCMNMRIVYDCPVVGCKGIK-GDTDPNTNTPRCRACTICIPRCNWCGRC 739

Query: 864 IDETVHEETFCLDLRCIDCGKEISKCE 884
           IDETVHEETFCLDLRCIDCGKEISKCE
Sbjct: 841 IDETVHEETFCLDLRCIDCGKEISKCE 739

BLAST of Lsi04G006500 vs. TAIR 10
Match: AT3G26000.1 (Ribonuclease inhibitor )

HSP 1 Score: 244.2 bits (622), Expect = 3.9e-64
Identity = 122/259 (47.10%), Postives = 165/259 (63.71%), Query Frame = 0

Query: 623 HAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLTS 682
           H A+ F L +L  ++LL V  VCKSL +T   D   W++I+IC  L+ KIT++ LL+LT 
Sbjct: 201 HPAIGFCLYHLRGKDLLSVSMVCKSLHTTVCDDTLLWKHIHICRPLNEKITEEALLHLTE 260

Query: 683 KAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRGG 742
           +AQG ++ L +V+C  I+DD LK V+  N +V K+ VPGCTR+TI GI+  L+  KS G 
Sbjct: 261 RAQGTMQCLRIVDCCRITDDCLKRVVARNRQVVKIGVPGCTRITIDGILSVLRDLKSAGK 320

Query: 743 PGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAID 802
             +KHL + G++GVT+ H+ EL  LL  I+N      ++PRFY  GE    S +  RA+D
Sbjct: 321 LQVKHLQLRGLFGVTKDHYDELIDLL-NIDNKVKQTIQKPRFYHRGE-ACVSCDDDRALD 380

Query: 803 IERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCIDETVH 862
           IE CPKC N ++VYDCP   CKG K+G       +  CRAC+LCI RC  CGRCI +T +
Sbjct: 381 IEMCPKCQNFKLVYDCPAEDCKGKKKG-------SEECRACSLCIQRCYHCGRCIIDTEY 440

Query: 863 EETFCLDLRCIDCGKEISK 882
           EE FCL+L C  C K   K
Sbjct: 441 EEMFCLELLCAVCSKPTPK 450


HSP 2 Score: 90.5 bits (223), Expect = 7.3e-18
Identity = 75/208 (36.06%), Postives = 97/208 (46.63%), Query Frame = 0

Query: 1   MALNFSHRPIFPPHLTEENLVSSMSISSGFAVDGVSERNSDVYGTSWLINRELVDCLNFL 60
           MALNFSHRP F  HL+EE     M I++G                +W        C +F 
Sbjct: 1   MALNFSHRP-FSSHLSEE----PMMIANG----------------NW--------CSSF- 60

Query: 61  EDTCEVGRSRDCVPGDVLDLLPSDPFGMDMSTTFTAITGWLDDLNVDY-GRYGR------ 120
            D        D    D+LD+LPSDPFGMD++ TFTAITGWL+DL  DY  +YGR      
Sbjct: 61  -DNGRKNTGGDASSVDILDVLPSDPFGMDINNTFTAITGWLEDLEDDYNNQYGRRRRDDI 120

Query: 121 ----APNYELFAEFNYICNSAFRFQA--FSLGNEGIL---------------HSTGELGG 180
                   +LFA  ++  N+A +FQ+  +S G+E +                 S+GE  G
Sbjct: 121 WIGDGNRQQLFAGLSFFWNNAMQFQSSGYSYGSESLFGGAFDGSLFSTCKFPESSGENNG 173

BLAST of Lsi04G006500 vs. TAIR 10
Match: AT3G27290.1 (RNI-like superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 9.1e-45
Identity = 102/259 (39.38%), Postives = 141/259 (54.44%), Query Frame = 0

Query: 620 GPPHAALNFVLGYLGTRELLVVESVCKSLQSTAEGDPFFWRNINICGK-LDVKITDDVLL 679
           G  H A   VL YL  +E+L VE VC+SL+ +   +PFFW +I++    L  ++TD+ LL
Sbjct: 125 GEAHDAFELVLPYLELKEILAVEVVCRSLRDSVGKEPFFWTSIDLNDSFLQYRVTDESLL 184

Query: 680 NLTSKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFK 739
            LT +A GG+  L+L  CV I+D GLK+VL +NP +TKL V GC RL+  G+V  L+  K
Sbjct: 185 KLTRRALGGVRCLNLGGCVGITDYGLKQVLASNPHLTKLSVSGCLRLSTAGLVSTLRDLK 244

Query: 740 SRGGPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGG 799
           S    G+K L   G    T+  FKEL  LL+G +    L  R+ RFY      F   +  
Sbjct: 245 SSNRLGVKSLITGGALYFTKEQFKEL-NLLLGGDAKVGLQERKKRFYTSCRSEFYLED-D 304

Query: 800 RAIDIERCPKCMNMRIVYDCPVVGC--KGIKEGDTNPDANTPRCRACTLCIARCNWCGRC 859
           R  D+E CP C    +V+DCP   C  KG          +   CRAC +CI RC+ CG C
Sbjct: 305 RVTDLEICPWCEKPSLVFDCPADTCPLKG------QYPYSKSSCRACVVCIERCHECGSC 364

Query: 860 IDETVHEETFCLDLRCIDC 876
           +++    + FC    C+ C
Sbjct: 365 LND-CENKPFCFAFSCVVC 374

BLAST of Lsi04G006500 vs. TAIR 10
Match: AT2G01620.1 (RNI-like superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 5.9e-28
Identity = 83/265 (31.32%), Postives = 130/265 (49.06%), Query Frame = 0

Query: 623 HAALNFVLGYLGTR-ELLVVESVCKSLQSTAEGDPFFWRNINICGKLDVKITDDVLLNLT 682
           H  L  VL YL +  ELL +  V +SL+     +   W  + I   L  ++TDD+L   +
Sbjct: 17  HEVLLIVLPYLHSLFELLSMIRVSRSLRDAIRDETALWTKLVIEPPLSSRLTDDILSEFS 76

Query: 683 SKAQGGLESLSLVNCVMISDDGLKEVLLNNPKVTKLCVPGCTRLTIGGIVDNLKAFKSRG 742
           SK+ G L++L L  C+M+++ GL+ V+  NP +TK+ VPGC+ LT  GI++ +++  S+ 
Sbjct: 77  SKSAGKLKTLILRQCLMVTNKGLRRVVDANPLITKIIVPGCSGLTPEGIMECVESL-SKN 136

Query: 743 GPGIKHLSIAGMYGVTEVHFKELEKLLVGIENFTHLNAREPRFYRGGEPRFPSSNGGRAI 802
              ++ L I G+ G T+ H   L         +T+L                SS G   I
Sbjct: 137 NHKLETLHINGVNGFTKQHLSAL---------YTYL----------------SSEG--TI 196

Query: 803 DIERCPKCMNMRIVYDCPVVGCKGIKEGDTNPDANTPRCRACTLCIARCNWCGRCI--DE 862
           D+E CPKC  +R++  C    C          +    +CR C LCI RC  C  C+   +
Sbjct: 197 DLEVCPKCDEVRMIPSCSRESC----------NQKQRKCRGCWLCIPRCAECAVCLVGSD 243

Query: 863 TVHEETFC--LDLRCIDCGKEISKC 883
           T  +E  C   D+ C++C   + KC
Sbjct: 257 TESQEAACGNDDVLCLECWLVLPKC 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LU915.5e-6347.10F-box protein SKIP14 OS=Arabidopsis thaliana OX=3702 GN=SKIP14 PE=1 SV=1[more]
Q9LK241.3e-4339.38F-box protein At3g27290 OS=Arabidopsis thaliana OX=3702 GN=At3g27290 PE=2 SV=1[more]
Q9ZU908.3e-2731.32F-box protein SKIP28 OS=Arabidopsis thaliana OX=3702 GN=SKIP28 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQ130.0e+0073.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590200 PE=4 SV=1[more]
A0A6J1FZJ00.0e+0066.95uncharacterized protein LOC111449320 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5D3CZT60.0e+0072.66F-box protein SKIP14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
A0A6J1FZG40.0e+0067.48uncharacterized protein LOC111449320 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1FZG10.0e+0065.01F-box protein SKIP14-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114493... [more]
Match NameE-valueIdentityDescription
XP_038892073.10.0e+0076.40uncharacterized protein LOC120081355 isoform X1 [Benincasa hispida][more]
XP_004135509.10.0e+0073.48F-box protein SKIP14 [Cucumis sativus] >KGN51703.1 hypothetical protein Csa_0090... [more]
XP_038892074.10.0e+0073.71uncharacterized protein LOC120081355 isoform X2 [Benincasa hispida][more]
XP_022944934.10.0e+0066.95uncharacterized protein LOC111449320 isoform X1 [Cucurbita moschata][more]
KAA0034221.10.0e+0072.66F-box protein SKIP14 [Cucumis melo var. makuwa] >TYK15699.1 F-box protein SKIP14... [more]
Match NameE-valueIdentityDescription
AT3G26000.13.9e-6447.10Ribonuclease inhibitor [more]
AT3G27290.19.1e-4539.38RNI-like superfamily protein [more]
AT2G01620.15.9e-2831.32RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 638..781
e-value: 1.4E-9
score: 39.4
NoneNo IPR availablePANTHERPTHR13382:SF22F-BOX PROTEIN SKIP14coord: 1..883
NoneNo IPR availablePANTHERPTHR13382MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR Bcoord: 1..883
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 670..783
IPR001810F-box domainPROSITEPS50181FBOXcoord: 615..662
score: 8.570165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006500.1Lsi04G006500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding