Lsi04G006470 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G006470 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.8e-67
Identity = 149/436 (34.17%), Postives = 242/436 (55.50%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYP---AGSTTPAEIA 60
           MA Q++ILS+E I+PSSPTP+ L+ + L+ LDQV +  + P++ FYP     +   A+ +
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRS 60

Query: 61  DHLKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNK 120
           ++LK+SLS VLT +YPLAGR+N     +DCND GVPF+E RV+ +LS+ +      ELN+
Sbjct: 61  ENLKRSLSTVLTQFYPLAGRININS-SVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 120

Query: 121 YLPF-------ELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYC 180
           YLPF       E     +I + V+++ FECGG A+G+C+SHKI+DALS    +N W A C
Sbjct: 121 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATC 180

Query: 181 RGDKKALRAHLS-SAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPA 240
           + +   ++ +    +  FPP ++ +           V +R+  +   +E+++A+ A   +
Sbjct: 181 QEETDIVQPNFDLGSHHFPPMES-IPAPEFLPDENIVMKRFVFDKEKLEALKAQLAS--S 240

Query: 241 MENQRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPP 300
               +  +RV+ +   I+ +FI   +A              K   +   +VN+RSR++PP
Sbjct: 241 ATEVKNSSRVQIVIAVIWKQFIDVTRA----------KFDTKNKLVAAQAVNLRSRMNPP 300

Query: 301 VPDYAFGNYYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMK 360
            P  A GN     +AV  E    D    DLV   +  + KID +++K LQ+G  +LD   
Sbjct: 301 FPQSAMGNIATMAYAVAEE----DKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYEA 360

Query: 361 KTAAQFSTGELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVD 420
           +    F        SF+S CR+  YD DFGWGKP  + +  +  KNL  ++D ++ DG++
Sbjct: 361 EPQELF--------SFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGME 410

Query: 421 VYVHLKEEHMNKFEAD 426
            ++ + E+ M+   +D
Sbjct: 421 AWISMAEDEMSMLSSD 410

BLAST of Lsi04G006470 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 2.3e-62
Identity = 149/433 (34.41%), Postives = 233/433 (53.81%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYP---AGSTTPAEIA 60
           MA Q+E +S+E I PSSPTP+ L+ Y ++ LDQ+ +  + P + FYP     +  PA+ +
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS 60

Query: 61  DHLKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSD-VMNTPFPSELN 120
            HLK+SLS VLTH+YPLAGR+N     +DCND GVPF+E RV  +LS  + N     +L+
Sbjct: 61  QHLKQSLSKVLTHFYPLAGRINVNS-SVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 120

Query: 121 KYLPF------ELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYC 180
           +YLP       +++  +++ + V+++ FECGG A+G+ +SHKI+D LS    +N W A C
Sbjct: 121 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 180

Query: 181 RGDKKALRAHLS-SAELFPPTKTGLYNTRTSIF---RQRVARRYQINAADVESIRAKYAE 240
           RG+ + +  +   +A  FPP      NT +         V +R+     D E I A  A+
Sbjct: 181 RGETEIVLPNFDLAARHFPPVD----NTPSPELVPDENVVMKRF---VFDKEKIGALRAQ 240

Query: 241 CPAMENQRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRL 300
             +   ++  +RV+ +  +I+   I   +A              K  F+V  +VN+RSR+
Sbjct: 241 ASSASEEKNFSRVQLVVAYIWKHVIDVTRA----------KYGAKNKFVVVQAVNLRSRM 300

Query: 301 DPPVPDYAFGNYYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLD 360
           +PP+P YA GN     FA    +   D    DL+   R  + K + D+   L +G   L 
Sbjct: 301 NPPLPHYAMGNIATLLFAAVDAEW--DKDFPDLIGPLRTSLEKTEDDHNHELLKGMTCLY 360

Query: 361 SMKKTAAQFSTGELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGD 420
            ++         EL+  SFTS CR+  YD DFGWGKP    +     +N  +++D + GD
Sbjct: 361 ELEPQ-------ELL--SFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGD 404

BLAST of Lsi04G006470 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.4e-56
Identity = 156/471 (33.12%), Postives = 241/471 (51.17%), Query Frame = 0

Query: 5   IEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGSTTPAEIADH----- 64
           +E++SKETIKP +PTP  LR Y+++ LDQ +  VY P++ FYPA S   +  + H     
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 65  -LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKY 124
            LK+SLS+ L H+YP+AGR+      +DCND+G+ F E R+  R+ D M     + L+  
Sbjct: 69  LLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFM-MKSDAHLSLL 128

Query: 125 LPFEL---DELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYC----R 184
           LP E+   + + E  + VQ+N+F+CGG A+  C+S+KI+DA +    +   A       R
Sbjct: 129 LPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIARR 188

Query: 185 GDKKA-------LRAHLSSAELFPPTK----TGLYNTRTSIFRQR-VARRYQINAADVES 244
           G   A       L     S  LFPP++       Y T+ S    + V++R+  +AA + S
Sbjct: 189 GSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKITS 248

Query: 245 IRAKYAECPAMENQ-RRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCH 304
            R K       + +  RPTRVE +S  I+    +A+K           S     I  V H
Sbjct: 249 AREKLQSLMHDKYKCHRPTRVEVVSALIWK---SAVK-----------SAPPGSISTVTH 308

Query: 305 SVNIRSRLDPPVPDYAFGNYYRTTFAV----------PSEKILND---------DYCYDL 364
           ++N R ++DPP+ D +FGN      AV          P+ K ++          D   D 
Sbjct: 309 AMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDF 368

Query: 365 VKQAREEIGKI--DKDYLKRLQEGSKFLDSMKKTAAQFSTGE--LISCSFTSLCRMPIYD 424
           V   R EI K+  DK  ++++ +  KF+     + A+ S  E  + +   TS C+   Y+
Sbjct: 369 VALLRREIDKVKGDKGCMEKIIQ--KFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYE 428

BLAST of Lsi04G006470 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-51
Identity = 139/444 (31.31%), Postives = 231/444 (52.03%), Query Frame = 0

Query: 5   IEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAG-STTPAEIADHLKKS 64
           +EI+SK  IKPSSPTP+ LRRY+L+ +DQ        + +FY    S   ++I D LK S
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGDLLKNS 65

Query: 65  LSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYLPFEL 124
           LS  L  YY  AGR+   + +IDCND+GV F+E R++ R++D++        +  LP  +
Sbjct: 66  LSKTLVSYYQFAGRLIEND-YIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLPTRI 125

Query: 125 DELDEIS-MGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRAHLS 184
             L E S + VQL+ F+CGG+A+G   SHK+SD +S  + +  WA+            L 
Sbjct: 126 IALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKPTPLL 185

Query: 185 SAE-LFPPTKTGLYNTRTSI-FRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRVE 244
           +A+ +FPP    L + ++ + F+Q + +R+  +   +E +++K  E       ++P+RVE
Sbjct: 186 TADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YGIQKPSRVE 245

Query: 245 ALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYR 304
            ++ F+        +  ++  L        KK + +  +VN+R  L   +P  + GN + 
Sbjct: 246 VVTAFL-------CQCAANCDL------PRKKPYAIISAVNLRPYL--ALPQNSIGNIFS 305

Query: 305 TTFAVPSEKILNDDYCYDLVKQAREE--------IGKIDKDYLKRLQEGSKFLDSMKKTA 364
             F +  E +  D+    L+ + R          I K    Y  ++QE +K LD +    
Sbjct: 306 FYFCINDEGM--DNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQL---- 365

Query: 365 AQFSTGELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYV 424
              S+ +   CS  S CR P+YD DFGWGKP  +       K+L +++D  +GDG++  +
Sbjct: 366 -NISSLDTYFCS--SWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALI 420

Query: 425 HLKEEHMNKFEADEDFLKYAKLPS 437
            ++E+ M  FE +E+   +A L S
Sbjct: 426 TMEEKKMAAFEKNEELRSFAYLDS 420

BLAST of Lsi04G006470 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 5.4e-51
Identity = 148/477 (31.03%), Postives = 244/477 (51.15%), Query Frame = 0

Query: 5   IEILSKETIKPSSPTPEHLRRYSLAFLDQ-VTVDVYNPMVYFYPA------GSTTPAEIA 64
           +E++SKETIKP++PTP  L+ ++L+ LDQ   +  Y P++ FYPA      GS+   +  
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 65  DHLKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNK 124
           D LK SLS  L H+YP+AGR+      +DC+D G+ F + ++  ++ + M+ P    L++
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRM-IDNILVDCHDQGINFYKVKIRGKMCEFMSQP-DVPLSQ 128

Query: 125 YLPFELDELD---EISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDK 184
            LP E+       E  + VQ+N+F+CGG A+   VSHKI+DA +    +  WA+  +  +
Sbjct: 129 LLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSR 188

Query: 185 ----------KALRAHLSSAELFPPTK-----TGLYNTRTSIFRQ------RVARRYQIN 244
                     + L     SA LFPP++     +G+     S   +       V++R+  +
Sbjct: 189 SGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRFVFD 248

Query: 245 AADVESIRAKYAECPAMEN--QRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEK 304
            A + S+R K  +    +N   RR TRVE +++ I+    + +K+  +  L         
Sbjct: 249 FAKITSVREK-LQVLMHDNYKSRRQTRVEVVTSLIWK---SVMKSTPAGFLP-------- 308

Query: 305 KIFLVCHSVNIRSRLDPPVPDYAFGNY------------YRTTFAV---------PSEKI 364
              +V H+VN+R ++DPP+ D +FGN               TT AV          S+ +
Sbjct: 309 ---VVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVV 368

Query: 365 LNDDYCYDLVKQAREEIGKI--DKDYLKRLQEGSKFLDSMKKTAAQFSTGELISCSFTSL 424
           L++   +D + Q R EI K+  DK  L+++ +   F      +  + +  E+I+   +S 
Sbjct: 369 LHE--LHDFIAQMRSEIDKVKGDKGSLEKVIQ--NFASGHDASIKKINDVEVINFWISSW 428

BLAST of Lsi04G006470 vs. ExPASy TrEMBL
Match: A0A0A0KPY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.5e-229
Identity = 388/438 (88.58%), Postives = 413/438 (94.29%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADH 60
           MAV+IEI+SKETIKPSSPTPEHLRRYSL+FLDQVTVDVYNPMVYFYPA GS TPAEI+DH
Sbjct: 1   MAVEIEIVSKETIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LK SLS VLTHYYPLAG+VNY EFFIDCND+GVPFIET++NCRLSDVMNTPFPSE+NK+L
Sbjct: 61  LKTSLSHVLTHYYPLAGKVNYDEFFIDCNDNGVPFIETKINCRLSDVMNTPFPSEVNKFL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LDE+SMGVQLNVFECGGV +GICVSHKISDALS FIVVN WAAYCRG+K+ +RA
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVVIGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAELFPPTKTGLYNTRTSIFRQRVARRY+I+  +VESIRAKYAEC AMENQRRP+RV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGENVESIRAKYAECSAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALS FIYSRFIAAIKAVSS  LENG S SEKKIFLVCHSVNIRSRLDPPV DYAFGNYY
Sbjct: 241 EALSAFIYSRFIAAIKAVSSSELENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFAVPSE ILND+YCYDLVKQAR EIGKIDKDYLKRLQEGSKFLDSMKKTA QFSTGE
Sbjct: 301 RTTFAVPSENILNDNYCYDLVKQARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           L+SCSFTSLCRMPIYDADFGWGKP WI SPAL+FKNLFV IDKKDGDGVD+YVHLKEEHM
Sbjct: 361 LVSCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
           NKFE DE+FLKYAKLPSN
Sbjct: 421 NKFEVDEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. ExPASy TrEMBL
Match: A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.8e-226
Identity = 381/438 (86.99%), Postives = 414/438 (94.52%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGS-TTPAEIADH 60
           MA QI+I+SKETIKPSSPTP+HLRRYSL+FLDQVTVDVYNPMVYF+ AGS TTPAEIAD 
Sbjct: 1   MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIADR 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LK SLSDVLTHYYPLAGR+NY EFFIDCNDDGVPFIETRVNC LSDVMNTPFPSELN+ L
Sbjct: 61  LKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRLL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LD++SMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRG+K+ALRA
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEALRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAE+FPPTKT ++NTRTSIFRQRV RRY I+AA+VE+IRAKYAECPAMENQRRP+RV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALSTFIYSRFIAAIKAVS   LENGSSE+EKKIFLVCHSVNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFA PSE+ILND+YCYDLVKQ REEIGKI+KDYLKRLQE SKF++SMKKTA+QF TGE
Sbjct: 301 RTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           L+SCSFTSLCRMPIYD DFGWG+PDWI SPAL+FKNLFV IDKKDGDG+++YVHL EEHM
Sbjct: 361 LVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
            KFE DE+FLKYAKLPSN
Sbjct: 421 KKFEIDEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. ExPASy TrEMBL
Match: A0A1S3BDS3 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.8e-226
Identity = 381/438 (86.99%), Postives = 414/438 (94.52%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGS-TTPAEIADH 60
           MA QI+I+SKETIKPSSPTP+HLRRYSL+FLDQVTVDVYNPMVYF+ AGS TTPAEIAD 
Sbjct: 1   MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIADR 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LK SLSDVLTHYYPLAGR+NY EFFIDCNDDGVPFIETRVNC LSDVMNTPFPSELN+ L
Sbjct: 61  LKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRLL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LD++SMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRG+K+ALRA
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEALRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAE+FPPTKT ++NTRTSIFRQRV RRY I+AA+VE+IRAKYAECPAMENQRRP+RV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALSTFIYSRFIAAIKAVS   LENGSSE+EKKIFLVCHSVNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFA PSE+ILND+YCYDLVKQ REEIGKI+KDYLKRLQE SKF++SMKKTA+QF TGE
Sbjct: 301 RTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           L+SCSFTSLCRMPIYD DFGWG+PDWI SPAL+FKNLFV IDKKDGDG+++YVHL EEHM
Sbjct: 361 LVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
            KFE DE+FLKYAKLPSN
Sbjct: 421 KKFEIDEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. ExPASy TrEMBL
Match: A0A5D3CUW9 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001950 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.2e-225
Identity = 386/440 (87.73%), Postives = 413/440 (93.86%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADH 60
           MAVQIEI+SK  IKPSSPTPEHLRRYSL+FLDQVTVDVYNPMVYFYPA GS TPAEI+DH
Sbjct: 1   MAVQIEIVSKAIIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LKKSLS VLTHYYPLAG+VNY EFF+DCNDDGVPFIET++NCR+S VMNTPFPSELNK+L
Sbjct: 61  LKKSLSHVLTHYYPLAGKVNYDEFFVDCNDDGVPFIETKINCRMSGVMNTPFPSELNKFL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LDE+SMGVQLNVFECGGVAVGICVSHKISDALS FIVVN WAAYCRG+K+ +RA
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVAVGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAELFPPTKTGLYNTRTSIFRQRVARRY+I+  +VESIRAKYAEC AME+QRRPTRV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGGNVESIRAKYAECSAMESQRRPTRV 240

Query: 241 EALSTFIYSRFIAAIKA-VSSRGLENG-SSESEKKIFLVCHSVNIRSRLDPPVPDYAFGN 300
           EALS FIY RFIAAIKA VSS  LENG S+E EK IFLVCHSVNIRSRLDPPV DYAFGN
Sbjct: 241 EALSAFIYGRFIAAIKAVVSSSELENGRSTELEKNIFLVCHSVNIRSRLDPPVVDYAFGN 300

Query: 301 YYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFST 360
           YYRTTFAVP+E ILND YCYDLVKQAR+EIGKIDK+YLKRLQ+GSKFLDSMKKTAAQFST
Sbjct: 301 YYRTTFAVPAENILNDTYCYDLVKQARDEIGKIDKEYLKRLQQGSKFLDSMKKTAAQFST 360

Query: 361 GELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEE 420
           GELISCSFTSLCRMPIYDADFGWGKP WIGSPAL+FKNLFV IDKKDGDGVD+YVHLK+E
Sbjct: 361 GELISCSFTSLCRMPIYDADFGWGKPAWIGSPALMFKNLFVFIDKKDGDGVDIYVHLKQE 420

Query: 421 HMNKFEADEDFLKYAKLPSN 438
           HMNKFE DE+FLKYAKLPSN
Sbjct: 421 HMNKFEMDEEFLKYAKLPSN 440

BLAST of Lsi04G006470 vs. ExPASy TrEMBL
Match: A0A1S3BF32 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488921 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.2e-225
Identity = 386/440 (87.73%), Postives = 413/440 (93.86%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADH 60
           MAVQIEI+SK  IKPSSPTPEHLRRYSL+FLDQVTVDVYNPMVYFYPA GS TPAEI+DH
Sbjct: 1   MAVQIEIVSKAIIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LKKSLS VLTHYYPLAG+VNY EFF+DCNDDGVPFIET++NCR+S VMNTPFPSELNK+L
Sbjct: 61  LKKSLSHVLTHYYPLAGKVNYDEFFVDCNDDGVPFIETKINCRMSGVMNTPFPSELNKFL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LDE+SMGVQLNVFECGGVAVGICVSHKISDALS FIVVN WAAYCRG+K+ +RA
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVAVGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAELFPPTKTGLYNTRTSIFRQRVARRY+I+  +VESIRAKYAEC AME+QRRPTRV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGGNVESIRAKYAECSAMESQRRPTRV 240

Query: 241 EALSTFIYSRFIAAIKA-VSSRGLENG-SSESEKKIFLVCHSVNIRSRLDPPVPDYAFGN 300
           EALS FIY RFIAAIKA VSS  LENG S+E EK IFLVCHSVNIRSRLDPPV DYAFGN
Sbjct: 241 EALSAFIYGRFIAAIKAVVSSSELENGRSTELEKNIFLVCHSVNIRSRLDPPVVDYAFGN 300

Query: 301 YYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFST 360
           YYRTTFAVP+E ILND YCYDLVKQAR+EIGKIDK+YLKRLQ+GSKFLDSMKKTAAQFST
Sbjct: 301 YYRTTFAVPAENILNDTYCYDLVKQARDEIGKIDKEYLKRLQQGSKFLDSMKKTAAQFST 360

Query: 361 GELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEE 420
           GELISCSFTSLCRMPIYDADFGWGKP WIGSPAL+FKNLFV IDKKDGDGVD+YVHLK+E
Sbjct: 361 GELISCSFTSLCRMPIYDADFGWGKPAWIGSPALMFKNLFVFIDKKDGDGVDIYVHLKQE 420

Query: 421 HMNKFEADEDFLKYAKLPSN 438
           HMNKFE DE+FLKYAKLPSN
Sbjct: 421 HMNKFEMDEEFLKYAKLPSN 440

BLAST of Lsi04G006470 vs. NCBI nr
Match: XP_038893435.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 811.6 bits (2095), Expect = 3.3e-231
Identity = 393/437 (89.93%), Postives = 417/437 (95.42%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGSTTPAEIADHL 60
           MAV+I+I+SKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFY AGS TPAEIA+HL
Sbjct: 1   MAVEIQIISKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYTAGSATPAEIANHL 60

Query: 61  KKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYLP 120
           KKSLSDVLTHYYPLAGRVNY EFFIDCNDDG+PFIETRVNCRLSDVMNTPFPSELNK+LP
Sbjct: 61  KKSLSDVLTHYYPLAGRVNYDEFFIDCNDDGIPFIETRVNCRLSDVMNTPFPSELNKFLP 120

Query: 121 FELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRAH 180
           FELDELDE+SMGVQLNVFECGGVAVGIC+SHKISDALSFFIVVNGWAAYCRG+K+ALR H
Sbjct: 121 FELDELDEVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAYCRGEKEALRTH 180

Query: 181 LSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRVE 240
           LSSAELFPP KTGLYNTRTSIFRQRVARRYQI+A +VESIRAKYAECPAM+NQRR +RVE
Sbjct: 181 LSSAELFPPAKTGLYNTRTSIFRQRVARRYQIDAVNVESIRAKYAECPAMQNQRRASRVE 240

Query: 241 ALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYR 300
           ALS FIYSRFIAAIKAVS   +E+ SSESEKKIFLVCH+VNIRSRLDPPVP+YAFGNYYR
Sbjct: 241 ALSAFIYSRFIAAIKAVS---MEDRSSESEKKIFLVCHAVNIRSRLDPPVPEYAFGNYYR 300

Query: 301 TTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGEL 360
           +TFAVPSE ILND+YCYDLVK AREEIGKIDK YL+RLQE SKFLDSMKK AAQFSTGEL
Sbjct: 301 STFAVPSEGILNDNYCYDLVKLAREEIGKIDKHYLQRLQESSKFLDSMKKAAAQFSTGEL 360

Query: 361 ISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHMN 420
           ISCSFTSLCRMPIYD DFGWGKP+WI SPALIFKNLFV IDKKDGDGVD+YVHLKEEHMN
Sbjct: 361 ISCSFTSLCRMPIYDVDFGWGKPEWISSPALIFKNLFVFIDKKDGDGVDIYVHLKEEHMN 420

Query: 421 KFEADEDFLKYAKLPSN 438
           KFEADE+FLKYAKLPSN
Sbjct: 421 KFEADEEFLKYAKLPSN 434

BLAST of Lsi04G006470 vs. NCBI nr
Match: XP_004135508.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51705.1 hypothetical protein Csa_008756 [Cucumis sativus])

HSP 1 Score: 804.3 bits (2076), Expect = 5.2e-229
Identity = 388/438 (88.58%), Postives = 413/438 (94.29%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADH 60
           MAV+IEI+SKETIKPSSPTPEHLRRYSL+FLDQVTVDVYNPMVYFYPA GS TPAEI+DH
Sbjct: 1   MAVEIEIVSKETIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LK SLS VLTHYYPLAG+VNY EFFIDCND+GVPFIET++NCRLSDVMNTPFPSE+NK+L
Sbjct: 61  LKTSLSHVLTHYYPLAGKVNYDEFFIDCNDNGVPFIETKINCRLSDVMNTPFPSEVNKFL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LDE+SMGVQLNVFECGGV +GICVSHKISDALS FIVVN WAAYCRG+K+ +RA
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVVIGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAELFPPTKTGLYNTRTSIFRQRVARRY+I+  +VESIRAKYAEC AMENQRRP+RV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGENVESIRAKYAECSAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALS FIYSRFIAAIKAVSS  LENG S SEKKIFLVCHSVNIRSRLDPPV DYAFGNYY
Sbjct: 241 EALSAFIYSRFIAAIKAVSSSELENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFAVPSE ILND+YCYDLVKQAR EIGKIDKDYLKRLQEGSKFLDSMKKTA QFSTGE
Sbjct: 301 RTTFAVPSENILNDNYCYDLVKQARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           L+SCSFTSLCRMPIYDADFGWGKP WI SPAL+FKNLFV IDKKDGDGVD+YVHLKEEHM
Sbjct: 361 LVSCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
           NKFE DE+FLKYAKLPSN
Sbjct: 421 NKFEVDEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. NCBI nr
Match: XP_008446095.1 (PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034224.1 vinorine synthase-like [Cucumis melo var. makuwa] >TYK15696.1 vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 381/438 (86.99%), Postives = 414/438 (94.52%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGS-TTPAEIADH 60
           MA QI+I+SKETIKPSSPTP+HLRRYSL+FLDQVTVDVYNPMVYF+ AGS TTPAEIAD 
Sbjct: 1   MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIADR 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LK SLSDVLTHYYPLAGR+NY EFFIDCNDDGVPFIETRVNC LSDVMNTPFPSELN+ L
Sbjct: 61  LKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRLL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LD++SMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRG+K+ALRA
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEALRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAE+FPPTKT ++NTRTSIFRQRV RRY I+AA+VE+IRAKYAECPAMENQRRP+RV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALSTFIYSRFIAAIKAVS   LENGSSE+EKKIFLVCHSVNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFA PSE+ILND+YCYDLVKQ REEIGKI+KDYLKRLQE SKF++SMKKTA+QF TGE
Sbjct: 301 RTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           L+SCSFTSLCRMPIYD DFGWG+PDWI SPAL+FKNLFV IDKKDGDG+++YVHL EEHM
Sbjct: 361 LVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
            KFE DE+FLKYAKLPSN
Sbjct: 421 KKFEIDEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. NCBI nr
Match: XP_008446094.1 (PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034223.1 vinorine synthase-like [Cucumis melo var. makuwa] >TYK15697.1 vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 791.2 bits (2042), Expect = 4.6e-225
Identity = 386/440 (87.73%), Postives = 413/440 (93.86%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADH 60
           MAVQIEI+SK  IKPSSPTPEHLRRYSL+FLDQVTVDVYNPMVYFYPA GS TPAEI+DH
Sbjct: 1   MAVQIEIVSKAIIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LKKSLS VLTHYYPLAG+VNY EFF+DCNDDGVPFIET++NCR+S VMNTPFPSELNK+L
Sbjct: 61  LKKSLSHVLTHYYPLAGKVNYDEFFVDCNDDGVPFIETKINCRMSGVMNTPFPSELNKFL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LDE+SMGVQLNVFECGGVAVGICVSHKISDALS FIVVN WAAYCRG+K+ +RA
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVAVGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAELFPPTKTGLYNTRTSIFRQRVARRY+I+  +VESIRAKYAEC AME+QRRPTRV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGGNVESIRAKYAECSAMESQRRPTRV 240

Query: 241 EALSTFIYSRFIAAIKA-VSSRGLENG-SSESEKKIFLVCHSVNIRSRLDPPVPDYAFGN 300
           EALS FIY RFIAAIKA VSS  LENG S+E EK IFLVCHSVNIRSRLDPPV DYAFGN
Sbjct: 241 EALSAFIYGRFIAAIKAVVSSSELENGRSTELEKNIFLVCHSVNIRSRLDPPVVDYAFGN 300

Query: 301 YYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFST 360
           YYRTTFAVP+E ILND YCYDLVKQAR+EIGKIDK+YLKRLQ+GSKFLDSMKKTAAQFST
Sbjct: 301 YYRTTFAVPAENILNDTYCYDLVKQARDEIGKIDKEYLKRLQQGSKFLDSMKKTAAQFST 360

Query: 361 GELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEE 420
           GELISCSFTSLCRMPIYDADFGWGKP WIGSPAL+FKNLFV IDKKDGDGVD+YVHLK+E
Sbjct: 361 GELISCSFTSLCRMPIYDADFGWGKPAWIGSPALMFKNLFVFIDKKDGDGVDIYVHLKQE 420

Query: 421 HMNKFEADEDFLKYAKLPSN 438
           HMNKFE DE+FLKYAKLPSN
Sbjct: 421 HMNKFEMDEEFLKYAKLPSN 440

BLAST of Lsi04G006470 vs. NCBI nr
Match: XP_004135298.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51706.1 hypothetical protein Csa_007767 [Cucumis sativus])

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-220
Identity = 374/438 (85.39%), Postives = 410/438 (93.61%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGS-TTPAEIADH 60
           MAVQI+I+SKE IKPSSPTP+HLRRY L+FLDQVTVDVYNPMVYFY AGS TTP EIAD 
Sbjct: 1   MAVQIDIVSKEIIKPSSPTPDHLRRYCLSFLDQVTVDVYNPMVYFYVAGSATTPVEIADR 60

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYL 120
           LKKSLSD LT+YYPLAGR+N  E FIDCNDDGVPFIET VNC LSDVMNT FPSELNK L
Sbjct: 61  LKKSLSDFLTYYYPLAGRLNSDELFIDCNDDGVPFIETSVNCPLSDVMNTSFPSELNKLL 120

Query: 121 PFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDKKALRA 180
           PFELD+LD++SMGVQLNVFECGGVAVGICVSHKISDALSFFIVVN WAAYCRG+K+A+R 
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNEWAAYCRGEKEAVRV 180

Query: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMENQRRPTRV 240
           HLSSAE+FPPTKT ++NTRTSIFRQRV RRY I+AA+VE+IRAKYA+CPAMENQRRP+RV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYADCPAMENQRRPSRV 240

Query: 241 EALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300
           EALSTFIYSRFIAAIKAVS+  +ENGSS+SEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSNDRMENGSSDSEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 301 RTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSKFLDSMKKTAAQFSTGE 360
           RTTFAVPSE++LND+YCYDLVKQ REEIGKI+KDYLKRLQ+ SKF++SMKKTA+QF+TGE
Sbjct: 301 RTTFAVPSEEVLNDEYCYDLVKQVREEIGKINKDYLKRLQDSSKFIESMKKTASQFATGE 360

Query: 361 LISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHLKEEHM 420
           LISCSFTSLCRMPIYD DFGWG+PDWI SPALIFKNLFV IDKKDGDGV++YVHL EEHM
Sbjct: 361 LISCSFTSLCRMPIYDIDFGWGQPDWISSPALIFKNLFVFIDKKDGDGVEIYVHLTEEHM 420

Query: 421 NKFEADEDFLKYAKLPSN 438
            KFEADE+FLKYAKLPSN
Sbjct: 421 KKFEADEEFLKYAKLPSN 438

BLAST of Lsi04G006470 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 340.5 bits (872), Expect = 2.0e-93
Identity = 181/437 (41.42%), Postives = 266/437 (60.87%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLR-RYSLAFLDQVTVDVYNPMVYFY-PAGSTTPAEIAD 60
           M ++I + S+E +KPS     H    + L+FLDQ+   ++ P ++FY    + +  E +D
Sbjct: 1   MGLEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSD 60

Query: 61  HLKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKY 120
           H+K SLS++L  YYPLAGR+      + CND GV F+E + +C +S ++  P P+ELNK 
Sbjct: 61  HIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNKL 120

Query: 121 LPFELDELDEISMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGD-KKAL 180
            PFE  E+ ++ + VQL  FECGG+A+GI +SHK+ DALS  I VN WAA+ RG   + +
Sbjct: 121 HPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQTDEII 180

Query: 181 RAHLSSAELFPPTKTGLYNTRTSIFRQR-VARRYQINAADVESIRAKYAECPAMENQRRP 240
                 A++FPP      N  T I ++  V RR+    + VES+R +++       + R 
Sbjct: 181 TPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSG----NKKIRA 240

Query: 241 TRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPDYAFG 300
           TRVE LS FI+SRF+A+       G          KI+ + H VN+R + DP +PD  FG
Sbjct: 241 TRVEVLSVFIWSRFMASTNHDDKTG----------KIYTLIHPVNLRRQADPDIPDNMFG 300

Query: 301 NYYRTTFAVPSEKI-LNDDYCYDLVKQAREEIGKIDKDYLKRLQEGSK-FLDSMKKTAAQ 360
           N  R +  VP   I  ND+    LV Q REEI KID  Y+K+LQE ++  L+ + K A+ 
Sbjct: 301 NIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASG 360

Query: 361 FSTGELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGVDVYVHL 420
           F  GE++S SFTSLC+ P+Y+ADFGWGKP W+ S  + +KNL   ID K+GDG++ +++L
Sbjct: 361 FVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINL 420

Query: 421 KEEHMNKFEADEDFLKY 432
            +  M++FEADE+ L+Y
Sbjct: 421 DQNDMSRFEADEELLRY 423

BLAST of Lsi04G006470 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.8e-71
Identity = 150/446 (33.63%), Postives = 258/446 (57.85%), Query Frame = 0

Query: 3   VQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPA-GSTTPAEIADHLK 62
           ++++++S++ IKPSSPTP HL+++ L+ L+Q+   ++ PMV+FY A  S  P E    LK
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLK 60

Query: 63  KSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSELNKYLPF 122
           KSLS+ LTH+YPLAGR+  G   IDCND G  F+E RVN  LS+++  P    L + +P 
Sbjct: 61  KSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIPT 120

Query: 123 ELDELDEIS--MGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAY-CRGDKKALR 182
            +D ++  +  +  Q + FECG +++G+C+SHK++DA S  + +  WAA   RG  K + 
Sbjct: 121 SVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKTIG 180

Query: 183 AHL-SSAELFPP-----TKTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECPAMEN 242
           A +  + ++FPP     T          +  Q +++R+  +++ +++++AK +       
Sbjct: 181 APVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSFEV--- 240

Query: 243 QRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDPPVPD 302
             +PTRVEA+S  I+   + A + VS      G+S    K  ++ +SV++RSR+ PP   
Sbjct: 241 -NQPTRVEAVSALIWKSAMKATRTVS------GTS----KPSILANSVSLRSRVSPPFTK 300

Query: 303 YAFGNYYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRL---QEGSKFLDSMK 362
            + GN   + FA  +E+ +N      LV + R+   +    ++ +L      ++ + S +
Sbjct: 301 NSIGNLV-SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQ 360

Query: 363 KTAA-QFSTGELISCSFTSLCRMPIYDADFGWGKPDWIGSPALIFKNLFVIIDKKDGDGV 422
           K A    ++G+     F+S CR  +Y+ DFGWGKP W+G P++  KN+  ++D K+  G+
Sbjct: 361 KEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGI 420

Query: 423 DVYVHLKEEHMNKFEADEDFLKYAKL 435
           + +V+L E+ MN FE D + L++A L
Sbjct: 421 EAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of Lsi04G006470 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-55
Identity = 143/455 (31.43%), Postives = 238/455 (52.31%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAG-----STTPAE 60
           M   +EILS+E +KPSSPTP+  R  +L+ LD ++  +Y   + FY A        +  E
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEE 60

Query: 61  IADHLKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRVNCRLSDVMNTPFPSEL 120
            +  LKKSLS  L  +YPLAGR+  G  F++CND+G  FIE RV+  LS+ +  P P  L
Sbjct: 61  TSLKLKKSLSKTLPIFYPLAGRI-IGS-FVECNDEGAVFIEARVDHLLSEFLKCPVPESL 120

Query: 121 NKYLPFELDELDEIS---MGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRG 180
              +P E    + ++   + +Q N F CGG+ + ICVSHKI+DA S  + + GWA   RG
Sbjct: 121 ELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 180

Query: 181 DKKALRAHLSSAELFP------PTKTGLYNTRTSIFRQR-VARRYQINAADVESIRAKYA 240
               L    +++E+FP      P+K    + +  +     V +R+  +A+ ++ +RAK  
Sbjct: 181 LGITLIPSFTASEVFPKPLDELPSKP--MDRKEEVEEMSCVTKRFVFDASKIKKLRAK-- 240

Query: 241 ECPAMENQRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSR 300
              +    + PTRVEA+ T ++ R +  +  +SS            +  ++   VN+R +
Sbjct: 241 --ASRNLVKNPTRVEAV-TALFWRCVTKVSRLSS---------LTPRTSVLQILVNLRGK 300

Query: 301 LDPPVPDYAFGNYYRTTFAVPSEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQE----G 360
           +D  + +   GN            + N++   + ++   +EI +  + +    +E     
Sbjct: 301 VD-SLCENTIGNMLSLMI------LKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSS 360

Query: 361 SKFLDSMKKTAAQFSTG-ELISCSFTSLCRMPIYDADFGWGKPDWI-GSPALIFKNLFVI 420
           S+  + +++    +  G E+      S C++ +YDADFGWGKP W+ G     FKNL ++
Sbjct: 361 SRIFELLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLL 420

Query: 421 IDKKDGDGVDVYVHLKEEHMNKFEADEDFLKYAKL 435
           ID KDG+G++ ++ L EE M+ FE D++ L+ A L
Sbjct: 421 IDTKDGEGIEAWITLTEEQMSLFECDQELLESASL 430

BLAST of Lsi04G006470 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-55
Identity = 138/455 (30.33%), Postives = 232/455 (50.99%), Query Frame = 0

Query: 1   MAVQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGSTTPAEIADH- 60
           M +++E +SKE IKPSSPTP +L+   L+  D +   VY     FY        E   H 
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHK 63

Query: 61  LKKSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRV-NCRLSDVMNTPFPSELNKY 120
           LK SLS+ LT +YPLAGR+      +DC D+G  F++ RV NC L++ +  P    L + 
Sbjct: 64  LKTSLSETLTKFYPLAGRIT--GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 123

Query: 121 LPFELDELDEISMG------VQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRG 180
           LP ++ +   ++        V+   F CGG+A+GIC++HKI+DA S    +  WAA  RG
Sbjct: 124 LPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARG 183

Query: 181 DKKALRAH---LSSAELFPPTKTG--LYNTRTSIFRQRVARRYQINAADVESIRAKYAEC 240
           +  A        + A  +PP      L     +  R  + +R+   A+ VE +R K A  
Sbjct: 184 ENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAA-- 243

Query: 241 PAMENQRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLD 300
            + E   +PTRVE+++  I+  F+A+ K          ++  + K+ +     N+RS++ 
Sbjct: 244 -SEETVDQPTRVESVTALIWKCFVASSK----------TTTCDHKVLV--QLANLRSKIP 303

Query: 301 PPVPDYAFGNYYRTTFAVP---SEKILNDDYCYDLVKQAREEIGKIDKDYLKRLQE---- 360
             + + + GN   ++  +      ++  ++   DL K+ +EE+G +  D           
Sbjct: 304 SLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKK-KEELGTVILDEGGSSDSSSMI 363

Query: 361 GSKFLDSMKKTAAQFSTGELISCSFTSLCRMPIYDADFGWGKPDW-IGSPALIFKNLFVI 420
           GSK  + M    ++ S       + +S C++P+Y+A FGW  P W +G+ + +  NL ++
Sbjct: 364 GSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAML 423

Query: 421 IDKKDGDGVDVYVHLKEEHMNKFEADEDFLKYAKL 435
           ID KDG G++ +V L EE+M+ FE + + L +A +
Sbjct: 424 IDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of Lsi04G006470 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.5e-51
Identity = 135/452 (29.87%), Postives = 233/452 (51.55%), Query Frame = 0

Query: 3   VQIEILSKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYPAGSTTPAEIADH-LK 62
           +++E ++KE IKPSS TP  L+   L+  D +   VY     FY        E + H LK
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLK 63

Query: 63  KSLSDVLTHYYPLAGRVNYGEFFIDCNDDGVPFIETRV-NCRLSDVMNTPFPSELNKYLP 122
            SL++ LT +YPLAGR+      IDCND+G  F++ RV N  LSD + +P    L + LP
Sbjct: 64  TSLAETLTKFYPLAGRIK--GVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLP 123

Query: 123 FE-LDELDEIS-----MGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGDK 182
            + +D+  E +     + V+   F CGG+A+G+C++HKI+DA S    +  WAA  RG+ 
Sbjct: 124 LDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGEA 183

Query: 183 KALRAH--LSSAELFPPT----KTGLYNTRTSIFRQRVARRYQINAADVESIRAKYAECP 242
             + A    ++A  +PP     K  +      I   ++ +R+  +A+ +E +R K A   
Sbjct: 184 GDIVADPVFAAANFYPPANELFKFPVDENANKI--SQITKRFVFSASKLEELRTKAA--- 243

Query: 243 AMENQRRPTRVEALSTFIYSRFIAAIKAVSSRGLENGSSESEKKIFLVCHSVNIRSRLDP 302
           + +   RP RVE+++  ++  F+AA          + ++  + K+ +     N+R ++  
Sbjct: 244 SEDFVGRPKRVESVTALLWKAFVAA--------ASSNTNACDMKVLI--QPSNLRPKIPS 303

Query: 303 PVPDYAFGNYYRTTFAV---PSEKILNDDYCYDLVKQAREEIGKIDKDY--LKRLQEGSK 362
            + +   GN   ++  +     E+I  +    DL KQ  +++  + K+         GSK
Sbjct: 304 LLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQG-DDLQNVIKEEGGSSSAMIGSK 363

Query: 363 FLDSMKKTAAQFSTGELISCSFTSLCRMPIYDADFGWGKPDWI-GSPALIFKNLFVIIDK 422
             + M    ++ S       + +S C++P+Y+A FGWG P WI G+ A + +N+ ++ID 
Sbjct: 364 LANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDS 423

Query: 423 KDGDGVDVYVHLKEEHMNKFEADEDFLKYAKL 435
           KDG G++ +V L EE+M   E + + L +A +
Sbjct: 424 KDGQGIEAFVTLPEENMLSLEQNTELLAFASV 437