Lsi04G006460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G006460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionvinorine synthase-like
Locationchr04: 5921239 .. 5921769 (+)
RNA-Seq ExpressionLsi04G006460
SyntenyLsi04G006460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATGGAAGCTCAGAATCGGAGAAGAAGATATTCCTAGTATGCCACGCTGTAAACATACGCTCAAGACTAGACCCACCGGTCCCCGATTACGCATTTGGAAATTATTACAGATCCACATTTGCAGTTCCCTCAGAGGAGATTCTCAATGACAACTACTGTTATGGTCTTGTGAAGCAAGCGAGGGAAGAAATTGGGAAGATCAACGAAGACTATCTCAAAAGGCTTCAAGAAAGCTCCAAATTCCTCGACTACATGAAGAAAGCGGCCGCCCAATTCTCCACCGGAGAACTCATCTCTTGCTCTTTCACCAGCCTTTGTCGGATGCCCATTTACGACGTCGATTTCGGGTGGGGCAAACCCGATTGGATCAGCTCCCCGGCCTTGATCTTCAAGAATCTCTTTGTCTTCATTGACAAGAAAGATGGCGATGGAGTTGATGTTTATGTTCATCTCAAAGAAGAGCACATGAATAAGTTTGAAGCAGATGAAGAGTTCCTCATGTATGCCAAGTTGCCTACTATTTAA

mRNA sequence

ATGGAGAATGGAAGCTCAGAATCGGAGAAGAAGATATTCCTAGTATGCCACGCTGTAAACATACGCTCAAGACTAGACCCACCGGTCCCCGATTACGCATTTGGAAATTATTACAGATCCACATTTGCAGTTCCCTCAGAGGAGATTCTCAATGACAACTACTGTTATGGTCTTGTGAAGCAAGCGAGGGAAGAAATTGGGAAGATCAACGAAGACTATCTCAAAAGGCTTCAAGAAAGCTCCAAATTCCTCGACTACATGAAGAAAGCGGCCGCCCAATTCTCCACCGGAGAACTCATCTCTTGCTCTTTCACCAGCCTTTGTCGGATGCCCATTTACGACGTCGATTTCGGGTGGGGCAAACCCGATTGGATCAGCTCCCCGGCCTTGATCTTCAAGAATCTCTTTGTCTTCATTGACAAGAAAGATGGCGATGGAGTTGATGTTTATGTTCATCTCAAAGAAGAGCACATGAATAAGTTTGAAGCAGATGAAGAGTTCCTCATGTATGCCAAGTTGCCTACTATTTAA

Coding sequence (CDS)

ATGGAGAATGGAAGCTCAGAATCGGAGAAGAAGATATTCCTAGTATGCCACGCTGTAAACATACGCTCAAGACTAGACCCACCGGTCCCCGATTACGCATTTGGAAATTATTACAGATCCACATTTGCAGTTCCCTCAGAGGAGATTCTCAATGACAACTACTGTTATGGTCTTGTGAAGCAAGCGAGGGAAGAAATTGGGAAGATCAACGAAGACTATCTCAAAAGGCTTCAAGAAAGCTCCAAATTCCTCGACTACATGAAGAAAGCGGCCGCCCAATTCTCCACCGGAGAACTCATCTCTTGCTCTTTCACCAGCCTTTGTCGGATGCCCATTTACGACGTCGATTTCGGGTGGGGCAAACCCGATTGGATCAGCTCCCCGGCCTTGATCTTCAAGAATCTCTTTGTCTTCATTGACAAGAAAGATGGCGATGGAGTTGATGTTTATGTTCATCTCAAAGAAGAGCACATGAATAAGTTTGAAGCAGATGAAGAGTTCCTCATGTATGCCAAGTTGCCTACTATTTAA

Protein sequence

MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPTI
Homology
BLAST of Lsi04G006460 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.4e-20
Identity = 51/155 (32.90%), Postives = 83/155 (53.55%), Query Frame = 0

Query: 10  KKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKI 69
           K   +   AVN+RSR++PP P  A GN     +AV  E    D     LV   +  + KI
Sbjct: 268 KNKLVAAQAVNLRSRMNPPFPQSAMGNIATMAYAVAEE----DKDFSDLVGPLKTSLAKI 327

Query: 70  NEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWISSPA 129
           +++++K LQ+   +LDY  +    F        SF+S CR+  YD+DFGWGKP  + +  
Sbjct: 328 DDEHVKELQKGVTYLDYEAEPQELF--------SFSSWCRLGFYDLDFGWGKPVSVCTTT 387

Query: 130 LIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEAD 165
           +  KNL   +D ++ DG++ ++ + E+ M+   +D
Sbjct: 388 VPMKNLVYLMDTRNEDGMEAWISMAEDEMSMLSSD 410

BLAST of Lsi04G006460 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.0e-16
Identity = 54/172 (31.40%), Postives = 87/172 (50.58%), Query Frame = 0

Query: 10  KKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKI 69
           KK + +  AVN+R  L   +P  + GN +   F +  E +  DN    L+ + R    K+
Sbjct: 258 KKPYAIISAVNLRPYL--ALPQNSIGNIFSFYFCINDEGM--DNQFSALISKLRNGKQKL 317

Query: 70  NED--------YLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGK 129
            E+        Y  ++QE +K LD       Q +   L +   +S CR P+YD+DFGWGK
Sbjct: 318 LENIISKEKLTYESQMQELTKCLD-------QLNISSLDTYFCSSWCRFPVYDIDFGWGK 377

Query: 130 PDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKL 174
           P  +S      K+L + +D  +GDG++  + ++E+ M  FE +EE   +A L
Sbjct: 378 PILVSPFQPHVKDLILLMDSPEGDGIEALITMEEKKMAAFEKNEELRSFAYL 418

BLAST of Lsi04G006460 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 8.6e-16
Identity = 51/170 (30.00%), Postives = 82/170 (48.24%), Query Frame = 0

Query: 14  LVCHAVNIRSRLDPPVPDYAFGN-------------------YYRSTFAVPSEEILNDNY 73
           +V HAVN+R ++DPP+ D +FGN                     ++  +  SE  +  + 
Sbjct: 295 VVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHE 354

Query: 74  CYGLVKQAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYD 133
            +  + Q R EI K+  D     +    F      +  + +  E+I+   +S CRM +Y+
Sbjct: 355 LHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWCRMGLYE 414

Query: 134 VDFGWGKPDWISSPALI--FKNLFVFIDKKDGDGVDVYVHLKEEHMNKFE 163
           +DFGWGKP W++    I   KN F   D K G+G++V+    E+ M KFE
Sbjct: 415 IDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFE 464

BLAST of Lsi04G006460 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 8.6e-16
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 0

Query: 10  KKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKI 69
           K  F+V  AVN+RSR++PP+P YA GN     FA    E   D     L+   R  + K 
Sbjct: 267 KNKFVVVQAVNLRSRMNPPLPHYAMGNIATLLFAAVDAEW--DKDFPDLIGPLRTSLEKT 326

Query: 70  NEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWISSPA 129
            +D+   L +    L        +    EL+  SFTS CR+  YD+DFGWGKP    +  
Sbjct: 327 EDDHNHELLKGMTCL-------YELEPQELL--SFTSWCRLGFYDLDFGWGKPLSACTTT 386

Query: 130 LIFKNLFVFIDKKDGDGVDVYVHLKEEHM 159
              +N  + +D + GDGV+ ++ + E+ M
Sbjct: 387 FPKRNAALLMDTRSGDGVEAWLPMAEDEM 404

BLAST of Lsi04G006460 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.7e-14
Identity = 52/175 (29.71%), Postives = 82/175 (46.86%), Query Frame = 0

Query: 12  IFLVCHAVNIRSRLDPPVPDYAFGNY-------------------YRSTFAVPSEEILND 71
           I  V HA+N R ++DPP+ D +FGN                     +   +  +EE +  
Sbjct: 287 ISTVTHAMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVAL 346

Query: 72  NYCYGLVKQAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGE--LISCSFTSLCRM 131
           +     V   R EI K+  D     +   KF+     + A+ S  E  + +   TS C+ 
Sbjct: 347 DELSDFVALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKF 406

Query: 132 PIYDVDFGWGKPDWISSPALI---FKNLFVFIDKKDGDGVDVYVHLKEEHMNKFE 163
             Y+ DFGWG P W+++  LI   +KN+    D K G+G++V+V+  E+ M KFE
Sbjct: 407 GFYEADFGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWVNFLEDDMTKFE 461

BLAST of Lsi04G006460 vs. ExPASy TrEMBL
Match: A0A0A0KVC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590720 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.5e-87
Identity = 152/175 (86.86%), Postives = 167/175 (95.43%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           MENGSS+SEKKIFLVCH+VNIRSRLDPPVPDYAFGNYYR+TFAVPSEE+LND YCY LVK
Sbjct: 263 MENGSSDSEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYRTTFAVPSEEVLNDEYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           Q REEIGKIN+DYLKRLQ+SSKF++ MKK A+QF+TGELISCSFTSLCRMPIYD+DFGWG
Sbjct: 323 QVREEIGKINKDYLKRLQDSSKFIESMKKTASQFATGELISCSFTSLCRMPIYDIDFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           +PDWISSPALIFKNLFVFIDKKDGDGV++YVHL EEHM KFEADEEFL YAKLP+
Sbjct: 383 QPDWISSPALIFKNLFVFIDKKDGDGVEIYVHLTEEHMKKFEADEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. ExPASy TrEMBL
Match: A0A0A0KPY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.8e-85
Identity = 152/175 (86.86%), Postives = 161/175 (92.00%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           +ENG S SEKKIFLVCH+VNIRSRLDPPV DYAFGNYYR+TFAVPSE ILNDNYCY LVK
Sbjct: 263 LENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYYRTTFAVPSENILNDNYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           QAR EIGKI++DYLKRLQE SKFLD MKK A QFSTGEL+SCSFTSLCRMPIYD DFGWG
Sbjct: 323 QARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGELVSCSFTSLCRMPIYDADFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           KP WISSPAL+FKNLFVFIDKKDGDGVD+YVHLKEEHMNKFE DEEFL YAKLP+
Sbjct: 383 KPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHMNKFEVDEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. ExPASy TrEMBL
Match: A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.4e-85
Identity = 148/175 (84.57%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           +ENGSSE+EKKIFLVCH+VNIRSRLDPPVPDYAFGNYYR+TFA PSEEILND YCY LVK
Sbjct: 263 LENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYRTTFAPPSEEILNDEYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           Q REEIGKIN+DYLKRLQESSKF++ MKK A+QF TGEL+SCSFTSLCRMPIYD+DFGWG
Sbjct: 323 QVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGELVSCSFTSLCRMPIYDIDFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           +PDWISSPAL+FKNLFVFIDKKDGDG+++YVHL EEHM KFE DEEFL YAKLP+
Sbjct: 383 QPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHMKKFEIDEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. ExPASy TrEMBL
Match: A0A1S3BDS3 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.4e-85
Identity = 148/175 (84.57%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           +ENGSSE+EKKIFLVCH+VNIRSRLDPPVPDYAFGNYYR+TFA PSEEILND YCY LVK
Sbjct: 263 LENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYRTTFAPPSEEILNDEYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           Q REEIGKIN+DYLKRLQESSKF++ MKK A+QF TGEL+SCSFTSLCRMPIYD+DFGWG
Sbjct: 323 QVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGELVSCSFTSLCRMPIYDIDFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           +PDWISSPAL+FKNLFVFIDKKDGDG+++YVHL EEHM KFE DEEFL YAKLP+
Sbjct: 383 QPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHMKKFEIDEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. ExPASy TrEMBL
Match: A0A5D3CUW9 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001950 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.3e-82
Identity = 147/176 (83.52%), Postives = 161/176 (91.48%), Query Frame = 0

Query: 1   MENG-SSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLV 60
           +ENG S+E EK IFLVCH+VNIRSRLDPPV DYAFGNYYR+TFAVP+E ILND YCY LV
Sbjct: 264 LENGRSTELEKNIFLVCHSVNIRSRLDPPVVDYAFGNYYRTTFAVPAENILNDTYCYDLV 323

Query: 61  KQAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGW 120
           KQAR+EIGKI+++YLKRLQ+ SKFLD MKK AAQFSTGELISCSFTSLCRMPIYD DFGW
Sbjct: 324 KQARDEIGKIDKEYLKRLQQGSKFLDSMKKTAAQFSTGELISCSFTSLCRMPIYDADFGW 383

Query: 121 GKPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           GKP WI SPAL+FKNLFVFIDKKDGDGVD+YVHLK+EHMNKFE DEEFL YAKLP+
Sbjct: 384 GKPAWIGSPALMFKNLFVFIDKKDGDGVDIYVHLKQEHMNKFEMDEEFLKYAKLPS 439

BLAST of Lsi04G006460 vs. NCBI nr
Match: XP_038893435.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 334.7 bits (857), Expect = 4.7e-88
Identity = 160/175 (91.43%), Postives = 168/175 (96.00%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           ME+ SSESEKKIFLVCHAVNIRSRLDPPVP+YAFGNYYRSTFAVPSE ILNDNYCY LVK
Sbjct: 259 MEDRSSESEKKIFLVCHAVNIRSRLDPPVPEYAFGNYYRSTFAVPSEGILNDNYCYDLVK 318

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
            AREEIGKI++ YL+RLQESSKFLD MKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG
Sbjct: 319 LAREEIGKIDKHYLQRLQESSKFLDSMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 378

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           KP+WISSPALIFKNLFVFIDKKDGDGVD+YVHLKEEHMNKFEADEEFL YAKLP+
Sbjct: 379 KPEWISSPALIFKNLFVFIDKKDGDGVDIYVHLKEEHMNKFEADEEFLKYAKLPS 433

BLAST of Lsi04G006460 vs. NCBI nr
Match: XP_004135298.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51706.1 hypothetical protein Csa_007767 [Cucumis sativus])

HSP 1 Score: 331.3 bits (848), Expect = 5.2e-87
Identity = 152/175 (86.86%), Postives = 167/175 (95.43%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           MENGSS+SEKKIFLVCH+VNIRSRLDPPVPDYAFGNYYR+TFAVPSEE+LND YCY LVK
Sbjct: 263 MENGSSDSEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYRTTFAVPSEEVLNDEYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           Q REEIGKIN+DYLKRLQ+SSKF++ MKK A+QF+TGELISCSFTSLCRMPIYD+DFGWG
Sbjct: 323 QVREEIGKINKDYLKRLQDSSKFIESMKKTASQFATGELISCSFTSLCRMPIYDIDFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           +PDWISSPALIFKNLFVFIDKKDGDGV++YVHL EEHM KFEADEEFL YAKLP+
Sbjct: 383 QPDWISSPALIFKNLFVFIDKKDGDGVEIYVHLTEEHMKKFEADEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. NCBI nr
Match: XP_004135508.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51705.1 hypothetical protein Csa_008756 [Cucumis sativus])

HSP 1 Score: 325.1 bits (832), Expect = 3.7e-85
Identity = 152/175 (86.86%), Postives = 161/175 (92.00%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           +ENG S SEKKIFLVCH+VNIRSRLDPPV DYAFGNYYR+TFAVPSE ILNDNYCY LVK
Sbjct: 263 LENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYYRTTFAVPSENILNDNYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           QAR EIGKI++DYLKRLQE SKFLD MKK A QFSTGEL+SCSFTSLCRMPIYD DFGWG
Sbjct: 323 QARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGELVSCSFTSLCRMPIYDADFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           KP WISSPAL+FKNLFVFIDKKDGDGVD+YVHLKEEHMNKFE DEEFL YAKLP+
Sbjct: 383 KPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHMNKFEVDEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. NCBI nr
Match: XP_008446095.1 (PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034224.1 vinorine synthase-like [Cucumis melo var. makuwa] >TYK15696.1 vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 324.7 bits (831), Expect = 4.9e-85
Identity = 148/175 (84.57%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MENGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVK 60
           +ENGSSE+EKKIFLVCH+VNIRSRLDPPVPDYAFGNYYR+TFA PSEEILND YCY LVK
Sbjct: 263 LENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYYRTTFAPPSEEILNDEYCYDLVK 322

Query: 61  QAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWG 120
           Q REEIGKIN+DYLKRLQESSKF++ MKK A+QF TGEL+SCSFTSLCRMPIYD+DFGWG
Sbjct: 323 QVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGELVSCSFTSLCRMPIYDIDFGWG 382

Query: 121 KPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           +PDWISSPAL+FKNLFVFIDKKDGDG+++YVHL EEHM KFE DEEFL YAKLP+
Sbjct: 383 QPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHMKKFEIDEEFLKYAKLPS 437

BLAST of Lsi04G006460 vs. NCBI nr
Match: XP_008446094.1 (PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034223.1 vinorine synthase-like [Cucumis melo var. makuwa] >TYK15697.1 vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 1.9e-81
Identity = 147/176 (83.52%), Postives = 161/176 (91.48%), Query Frame = 0

Query: 1   MENG-SSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLV 60
           +ENG S+E EK IFLVCH+VNIRSRLDPPV DYAFGNYYR+TFAVP+E ILND YCY LV
Sbjct: 264 LENGRSTELEKNIFLVCHSVNIRSRLDPPVVDYAFGNYYRTTFAVPAENILNDTYCYDLV 323

Query: 61  KQAREEIGKINEDYLKRLQESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGW 120
           KQAR+EIGKI+++YLKRLQ+ SKFLD MKK AAQFSTGELISCSFTSLCRMPIYD DFGW
Sbjct: 324 KQARDEIGKIDKEYLKRLQQGSKFLDSMKKTAAQFSTGELISCSFTSLCRMPIYDADFGW 383

Query: 121 GKPDWISSPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKLPT 176
           GKP WI SPAL+FKNLFVFIDKKDGDGVD+YVHLK+EHMNKFE DEEFL YAKLP+
Sbjct: 384 GKPAWIGSPALMFKNLFVFIDKKDGDGVDIYVHLKQEHMNKFEMDEEFLKYAKLPS 439

BLAST of Lsi04G006460 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 162.5 bits (410), Expect = 3.0e-40
Identity = 77/162 (47.53%), Postives = 110/162 (67.90%), Query Frame = 0

Query: 11  KIFLVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEI-LNDNYCYGLVKQAREEIGKI 70
           KI+ + H VN+R + DP +PD  FGN  R +  VP   I  ND     LV Q REEI KI
Sbjct: 262 KIYTLIHPVNLRRQADPDIPDNMFGNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKI 321

Query: 71  NEDYLKRLQESSK-FLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWISSP 130
           +  Y+K+LQE ++  L+++ K A+ F  GE++S SFTSLC+ P+Y+ DFGWGKP W++S 
Sbjct: 322 DAVYVKKLQEDNRGHLEFLNKQASGFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASA 381

Query: 131 ALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMY 171
            + +KNL  FID K+GDG++ +++L +  M++FEADEE L Y
Sbjct: 382 RMSYKNLVAFIDTKEGDGIEAWINLDQNDMSRFEADEELLRY 423

BLAST of Lsi04G006460 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-23
Identity = 53/164 (32.32%), Postives = 90/164 (54.88%), Query Frame = 0

Query: 14  LVCHAVNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKINEDY 73
           ++ ++V++RSR+ PP    + GN   S FA  +EE +N      LV + R+   +  + +
Sbjct: 268 ILANSVSLRSRVSPPFTKNSIGNLV-SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIH 327

Query: 74  LKRL----QESSKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWISSPA 133
           + +L      +     Y K+A    ++G+     F+S CR  +Y+ DFGWGKP W+  P+
Sbjct: 328 IPKLVGNPNATEIICSYQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPS 387

Query: 134 LIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKL 174
           +  KN+   +D K+  G++ +V+L E+ MN FE D E L +A L
Sbjct: 388 VRQKNIVTLLDTKEAGGIEAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of Lsi04G006460 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-15
Identity = 50/161 (31.06%), Postives = 84/161 (52.17%), Query Frame = 0

Query: 19  VNIRSRLDPPVPDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKINEDYLKRLQ 78
           VN+R ++D  + +   GN   S   + +EE   +      ++   +EI +  E +    +
Sbjct: 277 VNLRGKVD-SLCENTIGNML-SLMILKNEEAAIER-----IQDVVDEIRRAKEIFSLNCK 336

Query: 79  E----SSKFLDYMKKAAAQFSTG-ELISCSFTSLCRMPIYDVDFGWGKPDWISSPALI-F 138
           E    SS+  + +++    +  G E+      S C++ +YD DFGWGKP W++      F
Sbjct: 337 EMSKSSSRIFELLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHF 396

Query: 139 KNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKL 174
           KNL + ID KDG+G++ ++ L EE M+ FE D+E L  A L
Sbjct: 397 KNLMLLIDTKDGEGIEAWITLTEEQMSLFECDQELLESASL 430

BLAST of Lsi04G006460 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-12
Identity = 46/167 (27.54%), Postives = 82/167 (49.10%), Query Frame = 0

Query: 20  NIRSRLDPPVPDYAFGNYYRSTFA--------VPSEEILNDNYCYGLVKQAREEIGKINE 79
           N+RS++   + + + GN   S+          V  EE + D      +++ +EE+G +  
Sbjct: 280 NLRSKIPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRD------LRKKKEELGTVIL 339

Query: 80  DYLKRLQES----SKFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDW-IS 139
           D       S    SK  + M    ++ S       + +S C++P+Y+  FGW  P W + 
Sbjct: 340 DEGGSSDSSSMIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVG 399

Query: 140 SPALIFKNLFVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKL 174
           + + +  NL + ID KDG G++ +V L EE+M+ FE + E L +A +
Sbjct: 400 NVSPVLGNLAMLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of Lsi04G006460 vs. TAIR 10
Match: AT4G15400.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.5e-12
Identity = 52/163 (31.90%), Postives = 86/163 (52.76%), Query Frame = 0

Query: 18  AVNIRSRLDPPV-PDYAFGNYYRSTFAVPSEEILNDNYCYGLVKQAREEIGKINEDYLKR 77
           A++IR R+   V P    GN  +S F++  ++  ++     +V   R+   ++NE   + 
Sbjct: 271 AMDIRLRIPSSVAPKDVIGN-LQSGFSL-KKDAESEFEIPEIVATFRKNKERVNEMIKES 330

Query: 78  LQESS---KFLDYMKKAAAQFSTGELISCSFTSLCRMPIYDVDFGWGKPDWIS-SPALIF 137
           LQ ++     L  M +  ++  + E+     +S CR P Y+VDFG G P W+  +   I+
Sbjct: 331 LQGNTIGQSLLSLMAETVSE--STEIDRYIMSSWCRKPFYEVDFGSGSPVWVGYASHTIY 390

Query: 138 KNL--FVFIDKKDGDGVDVYVHLKEEHMNKFEADEEFLMYAKL 174
            N+   V ID K+GDGV+ ++ L EE M+ F  D+E L YA L
Sbjct: 391 DNMVGVVLIDSKEGDGVEAWISLPEEDMSVFVDDQELLAYAVL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2P1GIW74.4e-2032.90Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
A0A2K8FQU55.0e-1631.40Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
Q94FT48.6e-1630.00Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
Q70PR78.6e-1634.23Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
I3PLR44.7e-1429.71(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
Match NameE-valueIdentityDescription
A0A0A0KVC62.5e-8786.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590720 PE=3 SV=1[more]
A0A0A0KPY91.8e-8586.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1[more]
A0A5D3CZ302.4e-8584.57Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A1S3BDS32.4e-8584.57vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1[more]
A0A5D3CUW99.3e-8283.52Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
Match NameE-valueIdentityDescription
XP_038893435.14.7e-8891.43stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
XP_004135298.15.2e-8786.86stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51706.1 hypothetical prot... [more]
XP_004135508.13.7e-8586.86stemmadenine O-acetyltransferase [Cucumis sativus] >KGN51705.1 hypothetical prot... [more]
XP_008446095.14.9e-8584.57PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034224.1 vinorine synthase... [more]
XP_008446094.11.9e-8183.52PREDICTED: vinorine synthase-like [Cucumis melo] >KAA0034223.1 vinorine synthase... [more]
Match NameE-valueIdentityDescription
AT1G24430.13.0e-4047.53HXXXD-type acyl-transferase family protein [more]
AT3G26040.11.5e-2332.32HXXXD-type acyl-transferase family protein [more]
AT1G24420.12.0e-1531.06HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.2e-1227.54HXXXD-type acyl-transferase family protein [more]
AT4G15400.14.5e-1231.90HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 10..167
e-value: 4.4E-25
score: 88.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 2..171
e-value: 1.1E-43
score: 150.8
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 7..172
NoneNo IPR availablePANTHERPTHR31623:SF46ANTHRANILATE N-BENZOYLTRANSFERASEcoord: 7..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006460.1Lsi04G006460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups