Lsi04G006370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G006370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMethyltransf_11 domain-containing protein
Locationchr04: 5842860 .. 5843636 (+)
RNA-Seq ExpressionLsi04G006370
SyntenyLsi04G006370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGTATTCAAACAAAACCTCTTGTTATATCCAAATACTTTGTATTATCTCTCTTTTTCTCTCTTCCTTTCCTCCTCCTCCTCTCTCTCCGCCCTTACTACCACCGATTCTCCACGGCGGCCACCAACATTCCGCCGTCCGACAAATCCAACTCCGAATCTACCGGTTTAAGGATCCGACCCGGTTACACTTCCTACGAACGCTACATCAAGAAGCAACTCAACAAAACCCTAGACCCGAAGCTCCGAAAGATATGGACTACCAGAGATTGGGATCGGAAAATCCAAGTATTCTCCAGATTCTTCGACGGTTTGAAAGGGGAAGGGCTTCTTTCGAAGGAATCGAAAGCCCTATGCATCGGCGCTCGGGTCGGGCAAGAAGTGGAGGCGTTGAGGAAGATCGGAGTTTCCGATTCGATCGGAATGGACCTGGTTCCTTACCCGCCGCTTGTGGTTGAAGGCGATTTTCATAATCAGCCGTTTGGGAACAACAGTTTCGATTTCGAATTCTCCAATGTCTTCGATCACGCGCTCTACCCGGAGCGATTCGTGGCGGAGATCGAACGGACGTTGAAGCCCGGCGGGATTTGCGTCTTACACGTGGCGTTATCGCGACAGACAGATAAGTACTCCGCCAATGATCTTTACAGTGTAAAGCCGTTGGAGGCTCTGTTTCGGACATCGACGGTGGTCCGCGTTGGGAAGATCGACGGCTTTGGATTGGACACGGAGATTGTCTTCCGGAAAACGACGAACACGATCCAACGGTCTTGA

mRNA sequence

ATGAAAGGTATTCAAACAAAACCTCTTGTTATATCCAAATACTTTGTATTATCTCTCTTTTTCTCTCTTCCTTTCCTCCTCCTCCTCTCTCTCCGCCCTTACTACCACCGATTCTCCACGGCGGCCACCAACATTCCGCCGTCCGACAAATCCAACTCCGAATCTACCGGTTTAAGGATCCGACCCGGTTACACTTCCTACGAACGCTACATCAAGAAGCAACTCAACAAAACCCTAGACCCGAAGCTCCGAAAGATATGGACTACCAGAGATTGGGATCGGAAAATCCAAGTATTCTCCAGATTCTTCGACGGTTTGAAAGGGGAAGGGCTTCTTTCGAAGGAATCGAAAGCCCTATGCATCGGCGCTCGGGTCGGGCAAGAAGTGGAGGCGTTGAGGAAGATCGGAGTTTCCGATTCGATCGGAATGGACCTGGTTCCTTACCCGCCGCTTGTGGTTGAAGGCGATTTTCATAATCAGCCGTTTGGGAACAACAGTTTCGATTTCGAATTCTCCAATGTCTTCGATCACGCGCTCTACCCGGAGCGATTCGTGGCGGAGATCGAACGGACGTTGAAGCCCGGCGGGATTTGCGTCTTACACGTGGCGTTATCGCGACAGACAGATAAGTACTCCGCCAATGATCTTTACAGTGTAAAGCCGTTGGAGGCTCTGTTTCGGACATCGACGGTGGTCCGCGTTGGGAAGATCGACGGCTTTGGATTGGACACGGAGATTGTCTTCCGGAAAACGACGAACACGATCCAACGGTCTTGA

Coding sequence (CDS)

ATGAAAGGTATTCAAACAAAACCTCTTGTTATATCCAAATACTTTGTATTATCTCTCTTTTTCTCTCTTCCTTTCCTCCTCCTCCTCTCTCTCCGCCCTTACTACCACCGATTCTCCACGGCGGCCACCAACATTCCGCCGTCCGACAAATCCAACTCCGAATCTACCGGTTTAAGGATCCGACCCGGTTACACTTCCTACGAACGCTACATCAAGAAGCAACTCAACAAAACCCTAGACCCGAAGCTCCGAAAGATATGGACTACCAGAGATTGGGATCGGAAAATCCAAGTATTCTCCAGATTCTTCGACGGTTTGAAAGGGGAAGGGCTTCTTTCGAAGGAATCGAAAGCCCTATGCATCGGCGCTCGGGTCGGGCAAGAAGTGGAGGCGTTGAGGAAGATCGGAGTTTCCGATTCGATCGGAATGGACCTGGTTCCTTACCCGCCGCTTGTGGTTGAAGGCGATTTTCATAATCAGCCGTTTGGGAACAACAGTTTCGATTTCGAATTCTCCAATGTCTTCGATCACGCGCTCTACCCGGAGCGATTCGTGGCGGAGATCGAACGGACGTTGAAGCCCGGCGGGATTTGCGTCTTACACGTGGCGTTATCGCGACAGACAGATAAGTACTCCGCCAATGATCTTTACAGTGTAAAGCCGTTGGAGGCTCTGTTTCGGACATCGACGGTGGTCCGCGTTGGGAAGATCGACGGCTTTGGATTGGACACGGAGATTGTCTTCCGGAAAACGACGAACACGATCCAACGGTCTTGA

Protein sequence

MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRIRPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALCIGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALYPERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGFGLDTEIVFRKTTNTIQRS
Homology
BLAST of Lsi04G006370 vs. ExPASy TrEMBL
Match: A0A5D3CXL1 (Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001820 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.4e-126
Identity = 231/258 (89.53%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MK  Q+KPL+ISKYFVLSLFFSLPFLLLLSLRPYYHRFS+  TNIP  D        L+I
Sbjct: 1   MKPSQSKPLIISKYFVLSLFFSLPFLLLLSLRPYYHRFSSPPTNIPTPD--------LKI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSYE YIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLK EGL+S ESKALC
Sbjct: 61  RPGYTSYEHYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKREGLISTESKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEAL+KIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVEALKKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFR S VVR+GKIDGF
Sbjct: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRRSMVVRIGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTEIVFRKT NTIQRS
Sbjct: 241 GLDTEIVFRKTANTIQRS 250

BLAST of Lsi04G006370 vs. ExPASy TrEMBL
Match: A0A0A0KVD5 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G592800 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.3e-126
Identity = 231/258 (89.53%), Postives = 239/258 (92.64%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MK  Q+KPL+ISKYFVLSLFFSLPFLLLLSLRPYYHRFS+  T+IP  D        L+I
Sbjct: 1   MKPNQSKPLIISKYFVLSLFFSLPFLLLLSLRPYYHRFSSPPTDIPTPD--------LKI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSYE YIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLK EGL+S ESKALC
Sbjct: 61  RPGYTSYEHYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKREGLISTESKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEAL+KIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVEALKKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFR+STVVRVGKIDGF
Sbjct: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRSSTVVRVGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTEIVFRKT N IQRS
Sbjct: 241 GLDTEIVFRKTANIIQRS 250

BLAST of Lsi04G006370 vs. ExPASy TrEMBL
Match: A0A6J1GZ07 (uncharacterized protein LOC111458440 OS=Cucurbita moschata OX=3662 GN=LOC111458440 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.7e-124
Identity = 225/258 (87.21%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MKGIQTKPL ISKYFVLSLFFSLPFLLLLSLRPYYHRFST AT     D S  + + LRI
Sbjct: 1   MKGIQTKPLQISKYFVLSLFFSLPFLLLLSLRPYYHRFSTPAT-----DNSTPQPSDLRI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSY+RYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFF+GLKGEGLLS++SKALC
Sbjct: 61  RPGYTSYDRYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFEGLKGEGLLSEDSKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEV+ALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGN++FDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVQALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNHTFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           P+RFVAEIERTLKPGG+CVLHVALS  TDKYSANDL+SVKPLEALF+ S +VRVGKIDGF
Sbjct: 181 PDRFVAEIERTLKPGGVCVLHVALSGHTDKYSANDLHSVKPLEALFQRSAMVRVGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTE+VFRKTT+ IQRS
Sbjct: 241 GLDTEVVFRKTTDAIQRS 253

BLAST of Lsi04G006370 vs. ExPASy TrEMBL
Match: A0A6J1K870 (uncharacterized protein LOC111493119 OS=Cucurbita maxima OX=3661 GN=LOC111493119 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.5e-124
Identity = 225/258 (87.21%), Postives = 239/258 (92.64%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MKGIQTKPL ISKYFVLSLFFSLPFLLLLSLRPYYHRFST AT     D S  + + LRI
Sbjct: 1   MKGIQTKPLQISKYFVLSLFFSLPFLLLLSLRPYYHRFSTPAT-----DNSTPQPSDLRI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSY+RYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFF+GLKGEGLLS++SKALC
Sbjct: 61  RPGYTSYDRYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFEGLKGEGLLSEDSKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEV+ALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGN++FDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVQALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNHTFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           P+RFVAEIERTLKPGG+CVLHVALS  TDKYSANDL+SVKPLEALFR S VVRVGKIDGF
Sbjct: 181 PDRFVAEIERTLKPGGVCVLHVALSGHTDKYSANDLHSVKPLEALFRRSAVVRVGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTE+VFRK  + IQRS
Sbjct: 241 GLDTEVVFRKRRDAIQRS 253

BLAST of Lsi04G006370 vs. ExPASy TrEMBL
Match: A0A6J1G0Q0 (uncharacterized protein LOC111449615 OS=Cucurbita moschata OX=3662 GN=LOC111449615 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.5e-124
Identity = 225/258 (87.21%), Postives = 236/258 (91.47%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MKG QTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHR STAAT     D    ES  L+I
Sbjct: 39  MKGTQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRLSTAAT-----DNPQPESGDLKI 98

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSY RYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFF+GLKGEGL+S ESKALC
Sbjct: 99  RPGYTSYNRYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFEGLKGEGLISPESKALC 158

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEALR++GVSDSIGMDLVPYPPLV+ GDFHNQPFGN +FDFEFSNVFDHAL+
Sbjct: 159 IGARVGQEVEALRRVGVSDSIGMDLVPYPPLVIHGDFHNQPFGNETFDFEFSNVFDHALF 218

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGG+CVLHVALSRQTDKYSANDLYSV  LEALFR S VVRVGKIDGF
Sbjct: 219 PERFVAEIERTLKPGGVCVLHVALSRQTDKYSANDLYSVTALEALFRRSAVVRVGKIDGF 278

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTE+VFRKTTN I+RS
Sbjct: 279 GLDTEVVFRKTTNRIRRS 291

BLAST of Lsi04G006370 vs. NCBI nr
Match: XP_038891452.1 (uncharacterized protein LOC120080864 [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 4.4e-135
Identity = 245/259 (94.59%), Postives = 251/259 (96.91%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPP-SDKSNSESTGLR 60
           MKG QTKPLVISKYFVLSLFFSLPF+LLLSLRPYYHRFSTA TNIPP SDKSNSES  L+
Sbjct: 1   MKGAQTKPLVISKYFVLSLFFSLPFILLLSLRPYYHRFSTATTNIPPSSDKSNSESADLK 60

Query: 61  IRPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKAL 120
           IRPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGL+S ESKAL
Sbjct: 61  IRPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLISTESKAL 120

Query: 121 CIGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHAL 180
           CIGARVGQEVEALRKIGV DSIGMDLVPYPPLVVEGDFHNQPFGNN+FDFEFSNVFDHAL
Sbjct: 121 CIGARVGQEVEALRKIGVLDSIGMDLVPYPPLVVEGDFHNQPFGNNTFDFEFSNVFDHAL 180

Query: 181 YPERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDG 240
           YPERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPL+ALFR STVVRVGKIDG
Sbjct: 181 YPERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLKALFRRSTVVRVGKIDG 240

Query: 241 FGLDTEIVFRKTTNTIQRS 259
           FGLDTEIVFRKTT+TIQRS
Sbjct: 241 FGLDTEIVFRKTTSTIQRS 259

BLAST of Lsi04G006370 vs. NCBI nr
Match: TYK15684.1 (Methyltransferase type 11 [Cucumis melo var. makuwa])

HSP 1 Score: 461.5 bits (1186), Expect = 4.9e-126
Identity = 231/258 (89.53%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MK  Q+KPL+ISKYFVLSLFFSLPFLLLLSLRPYYHRFS+  TNIP  D        L+I
Sbjct: 1   MKPSQSKPLIISKYFVLSLFFSLPFLLLLSLRPYYHRFSSPPTNIPTPD--------LKI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSYE YIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLK EGL+S ESKALC
Sbjct: 61  RPGYTSYEHYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKREGLISTESKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEAL+KIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVEALKKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFR S VVR+GKIDGF
Sbjct: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRRSMVVRIGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTEIVFRKT NTIQRS
Sbjct: 241 GLDTEIVFRKTANTIQRS 250

BLAST of Lsi04G006370 vs. NCBI nr
Match: XP_004135292.1 (uncharacterized protein LOC101206192 [Cucumis sativus])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 231/258 (89.53%), Postives = 239/258 (92.64%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MK  Q+KPL+ISKYFVLSLFFSLPFLLLLSLRPYYHRFS+  T+IP  D        L+I
Sbjct: 1   MKPNQSKPLIISKYFVLSLFFSLPFLLLLSLRPYYHRFSSPPTDIPTPD--------LKI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSYE YIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLK EGL+S ESKALC
Sbjct: 61  RPGYTSYEHYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKREGLISTESKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEAL+KIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVEALKKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFR+STVVRVGKIDGF
Sbjct: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRSSTVVRVGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTEIVFRKT N IQRS
Sbjct: 241 GLDTEIVFRKTANIIQRS 250

BLAST of Lsi04G006370 vs. NCBI nr
Match: XP_022956890.1 (uncharacterized protein LOC111458440 [Cucurbita moschata] >KAG7032327.1 hypothetical protein SDJN02_06372, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 455.3 bits (1170), Expect = 3.5e-124
Identity = 225/258 (87.21%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MKGIQTKPL ISKYFVLSLFFSLPFLLLLSLRPYYHRFST AT     D S  + + LRI
Sbjct: 1   MKGIQTKPLQISKYFVLSLFFSLPFLLLLSLRPYYHRFSTPAT-----DNSTPQPSDLRI 60

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSY+RYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFF+GLKGEGLLS++SKALC
Sbjct: 61  RPGYTSYDRYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFEGLKGEGLLSEDSKALC 120

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEV+ALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGN++FDFEFSNVFDHALY
Sbjct: 121 IGARVGQEVQALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNHTFDFEFSNVFDHALY 180

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           P+RFVAEIERTLKPGG+CVLHVALS  TDKYSANDL+SVKPLEALF+ S +VRVGKIDGF
Sbjct: 181 PDRFVAEIERTLKPGGVCVLHVALSGHTDKYSANDLHSVKPLEALFQRSAMVRVGKIDGF 240

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTE+VFRKTT+ IQRS
Sbjct: 241 GLDTEVVFRKTTDAIQRS 253

BLAST of Lsi04G006370 vs. NCBI nr
Match: XP_022945362.1 (uncharacterized protein LOC111449615 [Cucurbita moschata])

HSP 1 Score: 453.4 bits (1165), Expect = 1.3e-123
Identity = 225/258 (87.21%), Postives = 236/258 (91.47%), Query Frame = 0

Query: 1   MKGIQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRI 60
           MKG QTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHR STAAT     D    ES  L+I
Sbjct: 39  MKGTQTKPLVISKYFVLSLFFSLPFLLLLSLRPYYHRLSTAAT-----DNPQPESGDLKI 98

Query: 61  RPGYTSYERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALC 120
           RPGYTSY RYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFF+GLKGEGL+S ESKALC
Sbjct: 99  RPGYTSYNRYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFEGLKGEGLISPESKALC 158

Query: 121 IGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALY 180
           IGARVGQEVEALR++GVSDSIGMDLVPYPPLV+ GDFHNQPFGN +FDFEFSNVFDHAL+
Sbjct: 159 IGARVGQEVEALRRVGVSDSIGMDLVPYPPLVIHGDFHNQPFGNETFDFEFSNVFDHALF 218

Query: 181 PERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 240
           PERFVAEIERTLKPGG+CVLHVALSRQTDKYSANDLYSV  LEALFR S VVRVGKIDGF
Sbjct: 219 PERFVAEIERTLKPGGVCVLHVALSRQTDKYSANDLYSVTALEALFRRSAVVRVGKIDGF 278

Query: 241 GLDTEIVFRKTTNTIQRS 259
           GLDTE+VFRKTTN I+RS
Sbjct: 279 GLDTEVVFRKTTNRIRRS 291

BLAST of Lsi04G006370 vs. TAIR 10
Match: AT1G24480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 5.7e-88
Identity = 156/244 (63.93%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 7   KPLVISKYFVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSESTGLRIRPGYTS 66
           +P  I KY ++S+F +LP +L  S++                     E   +RIRPGYTS
Sbjct: 3   QPKPILKYVLVSIFLTLPLILFFSIQ-----------------LRKPEKELIRIRPGYTS 62

Query: 67  YERYIKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALCIGARVG 126
           Y+ YI++QLNKTL+P+LR IW TRDWDRKI+VFSRFF  LK +GLLSK+SK LC+GARVG
Sbjct: 63  YDYYIQRQLNKTLNPRLRTIWMTRDWDRKIKVFSRFFQDLKRQGLLSKDSKCLCLGARVG 122

Query: 127 QEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALYPERFVA 186
           QEVEAL+++GV+DS+GMDLVPYPPLVV+GDFH+QPF + +FDFEFSNVFDHALYP++FV 
Sbjct: 123 QEVEALKRVGVNDSVGMDLVPYPPLVVKGDFHHQPFDDETFDFEFSNVFDHALYPDKFVG 182

Query: 187 EIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGFGLDTEI 246
           EIERTL+PGG+CVLHVALS ++DKYSANDL+SV+ L  LFR S VV V  +DGFGLDTE+
Sbjct: 183 EIERTLRPGGLCVLHVALSTRSDKYSANDLFSVEALVKLFRQSEVVHVRNVDGFGLDTEV 229

Query: 247 VFRK 251
           VFRK
Sbjct: 243 VFRK 229

BLAST of Lsi04G006370 vs. TAIR 10
Match: AT4G24805.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 5.9e-85
Identity = 163/243 (67.08%), Postives = 185/243 (76.13%), Query Frame = 0

Query: 15  FVLSLFFSLPFLLLLSLRPYYHRFSTAATNIPPSDKSNSEST---GLRIR-PGYTSYERY 74
           F LSLF SL FL++     +   F T     P S  S+  S    G+RIR  GY+SYE Y
Sbjct: 10  FTLSLFLSLSFLII-----FLFAFPTTFLRRPLSSSSSVTSVADEGIRIRHHGYSSYEAY 69

Query: 75  IKKQLNKTLDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALCIGARVGQEVE 134
           IK QLNKT +PKLRK+WTTRDWDRK++VFS FF  L   GLLS +SKAL IGARVGQEV 
Sbjct: 70  IKHQLNKTQNPKLRKVWTTRDWDRKVRVFSTFFRRLSDRGLLSNQSKALSIGARVGQEVA 129

Query: 135 ALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALYPERFVAEIER 194
           ALR IGV DS+G+DLVP PPLVV+GDFH QPF   +FDFEFSNVFDHALYPE+FV EIER
Sbjct: 130 ALRLIGVEDSVGIDLVPRPPLVVKGDFHAQPFDEETFDFEFSNVFDHALYPEKFVGEIER 189

Query: 195 TLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGFGLDTEIVFRK 254
           TLKPGG+CVLHV++S +TDKYSANDL SVKPL  LF+ S VV + KIDGFGLDTEIVFRK
Sbjct: 190 TLKPGGVCVLHVSISGKTDKYSANDLLSVKPLVKLFKRSKVVEMRKIDGFGLDTEIVFRK 247

BLAST of Lsi04G006370 vs. TAIR 10
Match: AT5G01710.1 (methyltransferases )

HSP 1 Score: 119.8 bits (299), Expect = 3.3e-27
Identity = 66/157 (42.04%), Postives = 89/157 (56.69%), Query Frame = 0

Query: 86  IWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALCIGARVGQEVEALRKIGVSDSIGMDL 145
           ++TTRDW + +Q +S  F  L  +G LS ESK LC+   +GQEV +LR+IGV +S+G+  
Sbjct: 81  LYTTRDWIKSVQFYSSIFQDLIADGYLSPESKTLCVETAIGQEVHSLREIGVKNSVGISK 140

Query: 146 VPYPPLVVEGDFHNQPFGNNSFDFEFS--NVFDHALYPERFVAEIERTLKPGGICVLHVA 205
               PLVV G+ H  PF +N+FDF FS  +    +L    F  EI RTLKP G  V+HV 
Sbjct: 141 KASRPLVVRGEGHAIPFEDNAFDFVFSGGDRLGKSLKQLEFADEITRTLKPEGFAVVHV- 200

Query: 206 LSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGF 241
                    A D YS      LF +  +V++  IDGF
Sbjct: 201 --------GATDTYSFNSFLDLFNSCRLVKMRDIDGF 228

BLAST of Lsi04G006370 vs. TAIR 10
Match: AT2G16030.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.4e-22
Identity = 62/172 (36.05%), Postives = 92/172 (53.49%), Query Frame = 0

Query: 79  LDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLLSKESKALCIGARVGQEVEALRKIGVS 138
           L  K  +IW++  W  ++  FS +F   +  G +   +KALC+ A  G  + AL +IG+S
Sbjct: 63  LPKKNARIWSSGAWKSRLYSFSTYFLRFRDLGFIQNHTKALCLSAGAGHALMALSQIGLS 122

Query: 139 DSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFSNVFDHALYPERFVAEIERTLKPGGIC 198
           D   ++LV   PLV   D HN PF +  FDF F+     AL+P +FV E+ERT++ GG C
Sbjct: 123 DVTAVELVDSIPLVKRADPHNLPFFDGVFDFAFTAHLAEALFPWQFVEEMERTVRRGGFC 182

Query: 199 VLHVALSRQTDKYSANDLYSVKPLEALFRTSTVVRVGKIDGFG-LDTEIVFR 250
           V+ V      D+   +D   V+ +  LF  S VV V  +   G   T I+F+
Sbjct: 183 VVSV------DECGGDD---VRDIARLFHNSKVVDVANVTLEGSKKTSILFK 225

BLAST of Lsi04G006370 vs. TAIR 10
Match: AT4G26730.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.1e-21
Identity = 62/185 (33.51%), Postives = 91/185 (49.19%), Query Frame = 0

Query: 68  ERYIKKQLNKT---------------LDPKLRKIWTTRDWDRKIQVFSRFFDGLKGEGLL 127
           ER ++K LN+                L  K  +IW +  W  ++  FS +F   +  G +
Sbjct: 2   ERNVEKMLNRVSVVFLSIGTVLMHLPLPKKNARIWFSGAWKSRLSSFSTYFLRFRDLGFI 61

Query: 128 SKESKALCIGARVGQEVEALRKIGVSDSIGMDLVPYPPLVVEGDFHNQPFGNNSFDFEFS 187
              +KALC+    G    AL +IG+SD   ++LV   PLV   D HN PF +  FDF F+
Sbjct: 62  QNHTKALCLSDGAGHAPMALSQIGLSDVTAVELVDSIPLVKRADPHNLPFFDRVFDFVFT 121

Query: 188 NVFDHALYPERFVAEIERTLKPGGICVLHVALSRQTDKYSANDLYSVKPLEALFRTSTVV 238
                AL+P RFV E+ERT++ GG CV+ V      D+ S +D   V+ +   F  S +V
Sbjct: 122 AHLAEALFPWRFVEEMERTVRRGGFCVVAV------DECSGDD---VRDIARFFHNSKIV 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CXL12.4e-12689.53Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0KVD55.3e-12689.53Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G59... [more]
A0A6J1GZ071.7e-12487.21uncharacterized protein LOC111458440 OS=Cucurbita moschata OX=3662 GN=LOC1114584... [more]
A0A6J1K8706.5e-12487.21uncharacterized protein LOC111493119 OS=Cucurbita maxima OX=3661 GN=LOC111493119... [more]
A0A6J1G0Q06.5e-12487.21uncharacterized protein LOC111449615 OS=Cucurbita moschata OX=3662 GN=LOC1114496... [more]
Match NameE-valueIdentityDescription
XP_038891452.14.4e-13594.59uncharacterized protein LOC120080864 [Benincasa hispida][more]
TYK15684.14.9e-12689.53Methyltransferase type 11 [Cucumis melo var. makuwa][more]
XP_004135292.11.1e-12589.53uncharacterized protein LOC101206192 [Cucumis sativus][more]
XP_022956890.13.5e-12487.21uncharacterized protein LOC111458440 [Cucurbita moschata] >KAG7032327.1 hypothet... [more]
XP_022945362.11.3e-12387.21uncharacterized protein LOC111449615 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G24480.15.7e-8863.93S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G24805.15.9e-8567.08S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G01710.13.3e-2742.04methyltransferases [more]
AT2G16030.11.4e-2236.05S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G26730.12.1e-2133.51S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 122..200
e-value: 2.9E-10
score: 40.7
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 79..223
e-value: 4.3E-11
score: 44.2
NoneNo IPR availablePANTHERPTHR45085:SF7S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN ISOFORM 1coord: 6..253
NoneNo IPR availablePANTHERPTHR45085F21J9.14coord: 6..253
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 152..204
e-value: 1.77712E-4
score: 38.1799
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 94..228

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006370.1Lsi04G006370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity