Lsi04G006220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G006220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cytochrome P450 71B37-like
Location	chr04: 5677055 .. 5678955 (-)
RNA-Seq Expression	Lsi04G006220
Synteny	Lsi04G006220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGTAAAGTTTCAACTCAATATAACACATCTCCCTTTCAAACCCCTTCACAAGGTTTTCACAAGTGGAAGACCAATCATTTATATTTGGATAATTTTTAAAAATAGAAAAATAAGGAAAATTATTTACACAAAATAGCAAAATTTTTAGATAGTTGTGTGATAGATGCTGAAAAAAGTTTATCAAGGTCTATCAGTGATAGAAATGATAGAAGTCTATCACTGATAGAAGTTTATCAATGTTTATCAGTGGTTTTTTTTGCTATTTTCTGTAAATACTTTGATATTTTTTTCTACCTGTGAAAATTTTCCTTTATATTTTTCCATATATCTTTTAAACTTTATGAGTTTTGATACCATATTTAAATAAATATGAAATTTTTATTTCAAGATTAATTAAATATGAAAGAGTAACTCGTGTCATATATATAATAATGCAAGTTTTGAATTCCTCTAGTTTTCTAATTTAGGATCTCGAAGATATTAAGATTTTTCTTGTATTCTCAATAAGTTATAGATTGCTAACCTTTGTGAGTTTTTTTCTTTCTTCTTATTTTCTTTTGAGTTCTTAATTTTTGGAAGGGTGTGTGTTTCCAGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

mRNA sequence

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

Coding sequence (CDS)

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

Protein sequence

MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWREVRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSFVVAKKSPLQLVPICYFNSI

Homology

[Close]

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot
Match: Q9LIP4 (Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 9.2e-120
Identity = 212/438 (48.40%), Postives = 301/438 (68.72%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK++LK++DL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+ +RVQSFQ I+++E+  L++S+S+S+S  T +NLSEKF S T+ +T + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
            FG  F+G  +++D  E +I    + L SFSA+D+FP  GWIIDWL+G H + E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LL+  +E+      K   + IKA+LM++L+ 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I K+  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  +P L+PR+ MS F+LN Y I  KT +Y+NVWAIGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  +FELL FGSGRR+CP M M T M+E  LANML  FDWK+P  M ++D+ +EE   
Sbjct: 426 DAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP+ Y +
Sbjct: 486 LNASKKNELVLVPLKYLD 499

[Close]

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot
Match: Q9LIP3 (Cytochrome P450 71B37 OS=Arabidopsis thaliana OX=3702 GN=CYP71B37 PE=3 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 3.0e-118
Identity = 215/438 (49.09%), Postives = 297/438 (67.81%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK+ LK++DL+CCSRP L      SY YLDIV   + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R++CV  LF+ ++V   Q IR+EE+  L+NS S+S++  T +NLSEK  S T+ +  + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F+G  +++DN + +I  A + L SFSA+D+FP VGWIIDWL+G   + E+S   L
Sbjct: 186 AFGVSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LLK  +E+      K   + IKA+LM++LL 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I  +  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLL+PRE MS F++NGY I  KT +Y+NVW IGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRR+CP M M T M+E  LAN+L  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP  Y N
Sbjct: 486 LNASKKNELVLVPRKYLN 499

[Close]

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 1.1e-117
Identity = 203/434 (46.77%), Postives = 297/434 (68.43%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           + L+LGSVP +VISSS AA+ +LK NDL CCSRP+    G+ SY + DI    YG++WRE
Sbjct: 66  VFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWRE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRK+ V+ LF++++VQSF+ IR+EE+D ++  +S+S+   + ++LS+ F+S T ++  R+
Sbjct: 126 VRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRV 185

Query: 121 AFGKKFR--GGEIDNDNIENVIRRAVVVLKSFSATDFFP-RVGWIIDWLSGFHTRMEKSF 180
           A G+ F   G  ID D IE ++  +   L +F+ +DFFP  +G  +DWL   H ++ K F
Sbjct: 186 ALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVF 245

Query: 181 TELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAG 240
            ELD ++QHV+D H+K       D    I+D++ K+   D  K + D +KA++MD+ LAG
Sbjct: 246 KELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAG 305

Query: 241 VETGANTLVWALTELVKNPKVMKKLQDEIRSC--IKEDRVKESDLEKLKYLKVVMKEVLR 300
           ++T A T++WA+TEL++NP+VMKK Q+ IR+   +K++R+ E DL K++YL  ++KE  R
Sbjct: 306 IDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   P ++PRETMSH K+ GYDI PKT + +NVW IGRDPK W +PEEF PERF  S +
Sbjct: 366 LHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSSV 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D++  +F+LL FGSGRRICP M MA   +EL L N+L  FDW +P   K +D+ +EE  +
Sbjct: 426 DFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGN 485

Query: 421 FVVAKKSPLQLVPI 430
             + KK PLQLVP+
Sbjct: 486 ISIVKKIPLQLVPV 499

[Close]

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot
Match: O64718 (Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 423.7 bits (1088), Expect = 2.5e-117
Identity = 214/436 (49.08%), Postives = 291/436 (66.74%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+V+SSS  AK++LK+NDL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+A+RV S Q I++EE+  L+ S ++S+S  + +NLSEKF   T+++  + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AF   F    ++ND  + +I  A + L SFSA++FFP  GWIIDWL+G   R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D ++Q + D H +  K       E+ VD+LLK  +E+      K   + +KA+LM++LL 
Sbjct: 246 DVFYQQMFDLHKQENK----QGVEDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRS-CIKEDRVKESDLEKLKYLKVVMKEVLR 300
            + T A T+ WA+ EL++NP+VMKK+Q EIR+  I +  +   D++ L YLK+V+KE  R
Sbjct: 306 AINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPRE MS F++NGY I PKTL+Y+NVWAIGRDP  W + + F PERF+ + I
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRRICP M M T M+E  LANML  FDW++P  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICY 432
             V KK+ L LVP+ Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

[Close]

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot
Match: Q9LTL0 (Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.5e-114
Identity = 214/438 (48.86%), Postives = 294/438 (67.12%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLG VPTL++SSS  AK+ L+  DL CCSRP L  G   SY  LD+    Y ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+C   LF+A ++QS Q I+ EE+  +++SI++SSS    +NLS+ F + T ++  + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F G  +++D    ++R    +L SFSA+DF P VGWIID  +G     +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H K  K  G +D   +VDVLL+  +E++     K   + IKA+LM+ILL 
Sbjct: 246 DAFYEQIFDLH-KEEKEVGSED---LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIK-EDRVKESDLEKLKYLKVVMKEVLR 300
           G++T A T+ WA+ EL KNP+VMKK+Q EIR+ IK ++R+   D +KL+YLK+V+KE  R
Sbjct: 306 GIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPR+ ++ F++NGY I  KT +++NVWAIGRDP  W +PE FLPERF  S I
Sbjct: 366 LHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELLSFGSGRRICP + M T M+E  LANML  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
             V+KKS L LVP+ Y +
Sbjct: 486 LTVSKKSELVLVPVKYLD 499

[Close]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL
Match: A0A5D3CWC3 (Cytochrome P450 71B37-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001670 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.6e-167
Identity = 301/446 (67.49%), Postives = 358/446 (80.27%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LGS+PT+V+SS+AAAKE+LKV+DL+ CSRPR  A  RFSY YLDI L  YG+HWRE
Sbjct: 45  MLLQLGSIPTVVVSSAAAAKEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWRE 104

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSS-SSATSINLSEKFYSFTINMTTR 120
           VRKICV+ LF+ARRVQSFQ IR+EEI V+LNSISQSS  S   I+LSEKFYS T N+ TR
Sbjct: 105 VRKICVLELFSARRVQSFQKIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITR 164

Query: 121 IAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTE 180
           IAFGKKFRGGE+DN+N + VIRRA+  + S SA D+FP+VGWIIDW++G H R+E SF E
Sbjct: 165 IAFGKKFRGGELDNENFQKVIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNE 224

Query: 181 LDGYFQHVLDYHIKFRKF------SGCDDEENIVDVLLKKAREDVP----KFDADCIKAL 240
           LD +FQH++D  IKFR+       +  D++ENIVDVLLK  +        K   DCIKAL
Sbjct: 225 LDIFFQHIVDDRIKFRESPRSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKAL 284

Query: 241 LMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKV 300
           +MDI LAGVETGA TLVWA+TEL+KNPKVMKKLQ+EIR+C+KE   VKESDL+ L+YLK 
Sbjct: 285 IMDIFLAGVETGATTLVWAMTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKA 344

Query: 301 VMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPE 360
           V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +++NVWAIGRD ++WTNPEEF+PE
Sbjct: 345 VVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPE 404

Query: 361 RFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDV 420
           RFIGS IDYK  NFE L FGSGRRICP MNMA+  +EL LANMLLCFDWKLP  +K +D+
Sbjct: 405 RFIGSNIDYKGQNFEFLPFGSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDI 464

Query: 421 GVEEDSSFVVAKKSPLQLVPICYFNS 435
            +EE+     +KKSPLQLVP+ YFNS
Sbjct: 465 DMEEELGLTASKKSPLQLVPVHYFNS 490

[Close]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL
Match: A0A1S4DW34 (cytochrome P450 71B37-like OS=Cucumis melo OX=3656 GN=LOC103488948 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.6e-167
Identity = 301/446 (67.49%), Postives = 358/446 (80.27%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LGS+PT+V+SS+AAAKE+LKV+DL+ CSRPR  A  RFSY YLDI L  YG+HWRE
Sbjct: 98  MLLQLGSIPTVVVSSAAAAKEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWRE 157

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSS-SSATSINLSEKFYSFTINMTTR 120
           VRKICV+ LF+ARRVQSFQ IR+EEI V+LNSISQSS  S   I+LSEKFYS T N+ TR
Sbjct: 158 VRKICVLELFSARRVQSFQKIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITR 217

Query: 121 IAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTE 180
           IAFGKKFRGGE+DN+N + VIRRA+  + S SA D+FP+VGWIIDW++G H R+E SF E
Sbjct: 218 IAFGKKFRGGELDNENFQKVIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNE 277

Query: 181 LDGYFQHVLDYHIKFRKF------SGCDDEENIVDVLLKKAREDVP----KFDADCIKAL 240
           LD +FQH++D  IKFR+       +  D++ENIVDVLLK  +        K   DCIKAL
Sbjct: 278 LDIFFQHIVDDRIKFRESPRSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKAL 337

Query: 241 LMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKV 300
           +MDI LAGVETGA TLVWA+TEL+KNPKVMKKLQ+EIR+C+KE   VKESDL+ L+YLK 
Sbjct: 338 IMDIFLAGVETGATTLVWAMTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKA 397

Query: 301 VMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPE 360
           V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +++NVWAIGRD ++WTNPEEF+PE
Sbjct: 398 VVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPE 457

Query: 361 RFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDV 420
           RFIGS IDYK  NFE L FGSGRRICP MNMA+  +EL LANMLLCFDWKLP  +K +D+
Sbjct: 458 RFIGSNIDYKGQNFEFLPFGSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDI 517

Query: 421 GVEEDSSFVVAKKSPLQLVPICYFNS 435
            +EE+     +KKSPLQLVP+ YFNS
Sbjct: 518 DMEEELGLTASKKSPLQLVPVHYFNS 543

[Close]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL
Match: A0A6J1DCP0 (cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019806 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.8e-161
Identity = 286/435 (65.75%), Postives = 348/435 (80.00%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+VISS+AAA+E+L+V+DL+CCSRPRL A  RFSY +LDI L  Y DHWRE
Sbjct: 45  MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWRE 104

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRKI V+ LF+ARRVQSFQ+IR+EE+ +LL S+ +SSSSA  ++LSEK Y+ T N+ TRI
Sbjct: 105 VRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRI 164

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK FRGGE+DN N   VIRRA+  L SFS TDFFP VGWIID +SG H R+EKSF EL
Sbjct: 165 AFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAEL 224

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D + QHV+D  I F   S   + ENI+DVLL+  RE    D  +   DCIKA++MDI +A
Sbjct: 225 DAFLQHVVDDRINFMATS---ENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIA 284

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GV  GA T++WA+ EL++NP+VMKKLQ EIRSCIKED+VKESDLEKL+YL++V+KEVLRL
Sbjct: 285 GVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRL 344

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           HA APLLLPRETMSHFKLNGYDI P   +++NVWAIGRDP  W+NPEEFLPERF+ S +D
Sbjct: 345 HAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVD 404

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           ++  NFELL FG+GRR CP +NM  I +EL LAN+LLCFDWKLP  MK +DV +EED+  
Sbjct: 405 FRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMK-EDVDMEEDAGL 464

Query: 421 VVAKKSPLQLVPICY 432
            VAKKSPL+LVP+CY
Sbjct: 465 TVAKKSPLKLVPVCY 475

[Close]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL
Match: A0A6J1DEX7 (cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019806 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.8e-161
Identity = 286/435 (65.75%), Postives = 348/435 (80.00%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+VISS+AAA+E+L+V+DL+CCSRPRL A  RFSY +LDI L  Y DHWRE
Sbjct: 51  MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWRE 110

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRKI V+ LF+ARRVQSFQ+IR+EE+ +LL S+ +SSSSA  ++LSEK Y+ T N+ TRI
Sbjct: 111 VRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRI 170

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK FRGGE+DN N   VIRRA+  L SFS TDFFP VGWIID +SG H R+EKSF EL
Sbjct: 171 AFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAEL 230

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D + QHV+D  I F   S   + ENI+DVLL+  RE    D  +   DCIKA++MDI +A
Sbjct: 231 DAFLQHVVDDRINFMATS---ENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIA 290

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GV  GA T++WA+ EL++NP+VMKKLQ EIRSCIKED+VKESDLEKL+YL++V+KEVLRL
Sbjct: 291 GVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRL 350

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           HA APLLLPRETMSHFKLNGYDI P   +++NVWAIGRDP  W+NPEEFLPERF+ S +D
Sbjct: 351 HAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVD 410

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           ++  NFELL FG+GRR CP +NM  I +EL LAN+LLCFDWKLP  MK +DV +EED+  
Sbjct: 411 FRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMK-EDVDMEEDAGL 470

Query: 421 VVAKKSPLQLVPICY 432
            VAKKSPL+LVP+CY
Sbjct: 471 TVAKKSPLKLVPVCY 481

[Close]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL
Match: A0A6J1GWP7 (cytochrome P450 71B26-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458149 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.5e-160
Identity = 287/436 (65.83%), Postives = 348/436 (79.82%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LGSVPT+VISS+AAA+E+ K +DL+ CSRPRLT  GRFSY +LD+ L  YG+ WRE
Sbjct: 71  MLLQLGSVPTIVISSAAAARELFKFHDLASCSRPRLTGSGRFSYNFLDLNLSPYGERWRE 130

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RKIC+  LF ARRVQSF  IR+EE+  L+ SISQSSSS+ SINLSEK YS T N+TTR+
Sbjct: 131 LRKICMHELFTARRVQSFWEIREEEVGRLVKSISQSSSSSASINLSEKSYSLTANITTRV 190

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F GG++D+++ ++VIRRAV  + SFS TDF P  GWIID L+G H R+EKSF E+
Sbjct: 191 AFGSIFSGGKLDDEHFQHVIRRAVAAIGSFSMTDFIPTFGWIIDRLTGVHGRLEKSFGEM 250

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D +FQHV+D  I F+K S   +EENIVDVLL+  RE    DV +   DC+KAL+MDI LA
Sbjct: 251 DAFFQHVVDDRINFKKSS--KNEENIVDVLLRMERESSESDVLQVTQDCVKALIMDIFLA 310

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GVETGA T+VW +TELV+NP+VMKKLQDEIRSCIKED VKE DLEKL+YLK+V+KE LRL
Sbjct: 311 GVETGATTIVWIMTELVRNPRVMKKLQDEIRSCIKEDSVKEMDLEKLEYLKMVVKEALRL 370

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           H   PLLLPR+T+S FKLNGY+IDPKT ++IN+WAIGRDP+ WT PEEF PERFIGS ID
Sbjct: 371 HPPVPLLLPRKTISSFKLNGYNIDPKTHLHINMWAIGRDPQSWTKPEEFFPERFIGSNID 430

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           YK  NFEL+ FGSGRRICP M+MAT+ +EL LAN+LLCFDWKLP  MK +DV +EE    
Sbjct: 431 YKGQNFELIPFGSGRRICPGMSMATLTVELALANLLLCFDWKLPDGMKEEDVDMEEADGL 490

Query: 421 VVAKKSPLQLVPICYF 433
              KKSPL+L+P+ YF
Sbjct: 491 AAPKKSPLRLIPVLYF 504

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: XP_038892966.1 (cytochrome P450 71B37-like [Benincasa hispida])

HSP 1 Score: 641.3 bits (1653), Expect = 5.8e-180
Identity = 324/440 (73.64%), Postives = 366/440 (83.18%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           M LKLGSVPT+VISS+ AAKE+LKV+DL+CCSRP+  AG RFSY YLDI L  YG++WRE
Sbjct: 81  MFLKLGSVPTVVISSADAAKEVLKVHDLACCSRPQSAAGARFSYNYLDIGLAPYGEYWRE 140

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRKICV+ LFN RRVQSFQMIR+EEI VLLNSISQSSSSAT I+LSEKFYS T NM TRI
Sbjct: 141 VRKICVLKLFNVRRVQSFQMIREEEIGVLLNSISQSSSSATPIDLSEKFYSLTANMITRI 200

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK FRGGE+DN+N + VIRRAV  L SFSATDFFPRVGWIID +SG H R+E SF EL
Sbjct: 201 AFGKSFRGGELDNENFQKVIRRAVAALGSFSATDFFPRVGWIIDRISGVHKRLEMSFAEL 260

Query: 181 DGYFQHVLDYHIKFRKFSGCDD--EENIVDVLLKKARE----DVPKFDADCIKALLMDIL 240
           D + QHV+D   KFR+ S  +D  +ENIVDVLLK  ++    DV K   DCIKAL+MDI 
Sbjct: 261 DAFLQHVVDDRTKFRESSSHNDDYQENIVDVLLKMEKDGSEFDVVKLTRDCIKALVMDIF 320

Query: 241 LAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVL 300
           L GVETGANTLVWA+TEL+KNPKVMKKLQDEIRSCI+E+ VKESDL+KL+YLK V+KEVL
Sbjct: 321 LGGVETGANTLVWAMTELIKNPKVMKKLQDEIRSCIRENVVKESDLDKLEYLKAVVKEVL 380

Query: 301 RLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSG 360
           RLH   PLLLPRETMSHFKLNGYDI PKT +++NVWAIGRDP+ WTNPE FLPERFIGS 
Sbjct: 381 RLHTPVPLLLPRETMSHFKLNGYDIHPKTHIHVNVWAIGRDPESWTNPEAFLPERFIGSN 440

Query: 361 IDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDS 420
           IDYK  NFELL FG+GRRICP M+MATI +EL LAN+LLCFDWKLP  MK KDV +EE+S
Sbjct: 441 IDYKGQNFELLPFGTGRRICPGMSMATITVELALANILLCFDWKLPNGMKEKDVDMEEES 500

Query: 421 SFVVAKKSPLQLVPICYFNS 435
              VAKKSPLQL+PI Y NS
Sbjct: 501 GIAVAKKSPLQLLPIPYLNS 520

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: KAA0034250.1 (cytochrome P450 71B37-like [Cucumis melo var. makuwa] >TYK15670.1 cytochrome P450 71B37-like [Cucumis melo var. makuwa])

HSP 1 Score: 599.0 bits (1543), Expect = 3.3e-167
Identity = 301/446 (67.49%), Postives = 358/446 (80.27%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LGS+PT+V+SS+AAAKE+LKV+DL+ CSRPR  A  RFSY YLDI L  YG+HWRE
Sbjct: 45  MLLQLGSIPTVVVSSAAAAKEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWRE 104

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSS-SSATSINLSEKFYSFTINMTTR 120
           VRKICV+ LF+ARRVQSFQ IR+EEI V+LNSISQSS  S   I+LSEKFYS T N+ TR
Sbjct: 105 VRKICVLELFSARRVQSFQKIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITR 164

Query: 121 IAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTE 180
           IAFGKKFRGGE+DN+N + VIRRA+  + S SA D+FP+VGWIIDW++G H R+E SF E
Sbjct: 165 IAFGKKFRGGELDNENFQKVIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNE 224

Query: 181 LDGYFQHVLDYHIKFRKF------SGCDDEENIVDVLLKKAREDVP----KFDADCIKAL 240
           LD +FQH++D  IKFR+       +  D++ENIVDVLLK  +        K   DCIKAL
Sbjct: 225 LDIFFQHIVDDRIKFRESPRSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKAL 284

Query: 241 LMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKV 300
           +MDI LAGVETGA TLVWA+TEL+KNPKVMKKLQ+EIR+C+KE   VKESDL+ L+YLK 
Sbjct: 285 IMDIFLAGVETGATTLVWAMTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKA 344

Query: 301 VMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPE 360
           V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +++NVWAIGRD ++WTNPEEF+PE
Sbjct: 345 VVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPE 404

Query: 361 RFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDV 420
           RFIGS IDYK  NFE L FGSGRRICP MNMA+  +EL LANMLLCFDWKLP  +K +D+
Sbjct: 405 RFIGSNIDYKGQNFEFLPFGSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDI 464

Query: 421 GVEEDSSFVVAKKSPLQLVPICYFNS 435
            +EE+     +KKSPLQLVP+ YFNS
Sbjct: 465 DMEEELGLTASKKSPLQLVPVHYFNS 490

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: XP_016900197.1 (PREDICTED: cytochrome P450 71B37-like [Cucumis melo])

HSP 1 Score: 599.0 bits (1543), Expect = 3.3e-167
Identity = 301/446 (67.49%), Postives = 358/446 (80.27%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LGS+PT+V+SS+AAAKE+LKV+DL+ CSRPR  A  RFSY YLDI L  YG+HWRE
Sbjct: 98  MLLQLGSIPTVVVSSAAAAKEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWRE 157

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSS-SSATSINLSEKFYSFTINMTTR 120
           VRKICV+ LF+ARRVQSFQ IR+EEI V+LNSISQSS  S   I+LSEKFYS T N+ TR
Sbjct: 158 VRKICVLELFSARRVQSFQKIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITR 217

Query: 121 IAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTE 180
           IAFGKKFRGGE+DN+N + VIRRA+  + S SA D+FP+VGWIIDW++G H R+E SF E
Sbjct: 218 IAFGKKFRGGELDNENFQKVIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNE 277

Query: 181 LDGYFQHVLDYHIKFRKF------SGCDDEENIVDVLLKKAREDVP----KFDADCIKAL 240
           LD +FQH++D  IKFR+       +  D++ENIVDVLLK  +        K   DCIKAL
Sbjct: 278 LDIFFQHIVDDRIKFRESPRSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKAL 337

Query: 241 LMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKV 300
           +MDI LAGVETGA TLVWA+TEL+KNPKVMKKLQ+EIR+C+KE   VKESDL+ L+YLK 
Sbjct: 338 IMDIFLAGVETGATTLVWAMTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKA 397

Query: 301 VMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPE 360
           V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +++NVWAIGRD ++WTNPEEF+PE
Sbjct: 398 VVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPE 457

Query: 361 RFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDV 420
           RFIGS IDYK  NFE L FGSGRRICP MNMA+  +EL LANMLLCFDWKLP  +K +D+
Sbjct: 458 RFIGSNIDYKGQNFEFLPFGSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDI 517

Query: 421 GVEEDSSFVVAKKSPLQLVPICYFNS 435
            +EE+     +KKSPLQLVP+ YFNS
Sbjct: 518 DMEEELGLTASKKSPLQLVPVHYFNS 543

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: XP_004135500.2 (cytochrome P450 71B37-like [Cucumis sativus] >KAE8648691.1 hypothetical protein Csa_009174 [Cucumis sativus])

HSP 1 Score: 598.6 bits (1542), Expect = 4.3e-167
Identity = 301/448 (67.19%), Postives = 356/448 (79.46%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+V+SS+ AAKE+LKV+DL+ CSRPR TA  RFSY YLDI    YGDHWR+
Sbjct: 80  MLLKLGSIPTVVVSSATAAKEVLKVHDLASCSRPRSTANARFSYNYLDIGFAPYGDHWRK 139

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSS--SSATSINLSEKFYSFTINMTT 120
           VRKI V+ LF+ARRVQSFQ IR+EEI V+LNSISQSS   S   ++LSEKFYS T NM T
Sbjct: 140 VRKISVLELFSARRVQSFQNIREEEIGVMLNSISQSSLLLSNDPVDLSEKFYSLTANMIT 199

Query: 121 RIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFT 180
           RIAFGKKFRGGE+DN+N + VIRRA+  + SFSA D+ P VGWIIDW+SG H+R+E SF 
Sbjct: 200 RIAFGKKFRGGELDNENFQKVIRRAMAAVGSFSAADYLPIVGWIIDWVSGVHSRLETSFN 259

Query: 181 ELDGYFQHVLDYHIKFRKFSGCD-------DEENIVDVLLKKAREDVP----KFDADCIK 240
           ELD +FQH++D  I FR+            D+ENIVDVLLK  +        K   DCIK
Sbjct: 260 ELDAFFQHIVDDRINFRESCSSHNNNGDGYDQENIVDVLLKMEKNSSQYGEVKLTRDCIK 319

Query: 241 ALLMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYL 300
           AL+MDI LAGVETGA+TLVW + EL+KNPKVMKKLQ+EIR+C+KE++ VKE+DL+ L+YL
Sbjct: 320 ALIMDIFLAGVETGASTLVWTMAELIKNPKVMKKLQNEIRNCVKENKMVKENDLQNLEYL 379

Query: 301 KVVMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFL 360
           K V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +Y+N WAIGRDP++WTNPEEF+
Sbjct: 380 KAVVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIYVNAWAIGRDPEIWTNPEEFI 439

Query: 361 PERFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSK 420
           PERFIGS IDYK  NFELL FGSGRRICP MNMA+  +EL LAN+LLCFDWKL   MK +
Sbjct: 440 PERFIGSNIDYKGQNFELLPFGSGRRICPGMNMASFTVELALANVLLCFDWKLANGMKEE 499

Query: 421 DVGVEEDSSFVVAKKSPLQLVPICYFNS 435
           DV +EE++   VAKKSPLQLVP+ YFNS
Sbjct: 500 DVDMEEETGLAVAKKSPLQLVPVHYFNS 527

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: XP_022151972.1 (cytochrome P450 71B34-like isoform X1 [Momordica charantia] >XP_022151976.1 cytochrome P450 71B34-like isoform X1 [Momordica charantia])

HSP 1 Score: 577.8 bits (1488), Expect = 7.9e-161
Identity = 286/435 (65.75%), Postives = 348/435 (80.00%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+VISS+AAA+E+L+V+DL+CCSRPRL A  RFSY +LDI L  Y DHWRE
Sbjct: 51  MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWRE 110

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRKI V+ LF+ARRVQSFQ+IR+EE+ +LL S+ +SSSSA  ++LSEK Y+ T N+ TRI
Sbjct: 111 VRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRI 170

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK FRGGE+DN N   VIRRA+  L SFS TDFFP VGWIID +SG H R+EKSF EL
Sbjct: 171 AFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAEL 230

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D + QHV+D  I F   S   + ENI+DVLL+  RE    D  +   DCIKA++MDI +A
Sbjct: 231 DAFLQHVVDDRINFMATS---ENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIA 290

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GV  GA T++WA+ EL++NP+VMKKLQ EIRSCIKED+VKESDLEKL+YL++V+KEVLRL
Sbjct: 291 GVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRL 350

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           HA APLLLPRETMSHFKLNGYDI P   +++NVWAIGRDP  W+NPEEFLPERF+ S +D
Sbjct: 351 HAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVD 410

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           ++  NFELL FG+GRR CP +NM  I +EL LAN+LLCFDWKLP  MK +DV +EED+  
Sbjct: 411 FRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMK-EDVDMEEDAGL 470

Query: 421 VVAKKSPLQLVPICY 432
            VAKKSPL+LVP+CY
Sbjct: 471 TVAKKSPLKLVPVCY 481

[Close]

BLAST of Lsi04G006220 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 431.8 bits (1109), Expect = 6.5e-121
Identity = 212/438 (48.40%), Postives = 301/438 (68.72%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK++LK++DL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+ +RVQSFQ I+++E+  L++S+S+S+S  T +NLSEKF S T+ +T + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
            FG  F+G  +++D  E +I    + L SFSA+D+FP  GWIIDWL+G H + E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LL+  +E+      K   + IKA+LM++L+ 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I K+  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  +P L+PR+ MS F+LN Y I  KT +Y+NVWAIGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  +FELL FGSGRR+CP M M T M+E  LANML  FDWK+P  M ++D+ +EE   
Sbjct: 426 DAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP+ Y +
Sbjct: 486 LNASKKNELVLVPLKYLD 499

[Close]

BLAST of Lsi04G006220 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 426.8 bits (1096), Expect = 2.1e-119
Identity = 215/438 (49.09%), Postives = 297/438 (67.81%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK+ LK++DL+CCSRP L      SY YLDIV   + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R++CV  LF+ ++V   Q IR+EE+  L+NS S+S++  T +NLSEK  S T+ +  + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F+G  +++DN + +I  A + L SFSA+D+FP VGWIIDWL+G   + E+S   L
Sbjct: 186 AFGVSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LLK  +E+      K   + IKA+LM++LL 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I  +  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLL+PRE MS F++NGY I  KT +Y+NVW IGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRR+CP M M T M+E  LAN+L  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP  Y N
Sbjct: 486 LNASKKNELVLVPRKYLN 499

[Close]

BLAST of Lsi04G006220 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 424.9 bits (1091), Expect = 8.0e-119
Identity = 203/434 (46.77%), Postives = 297/434 (68.43%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           + L+LGSVP +VISSS AA+ +LK NDL CCSRP+    G+ SY + DI    YG++WRE
Sbjct: 66  VFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWRE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRK+ V+ LF++++VQSF+ IR+EE+D ++  +S+S+   + ++LS+ F+S T ++  R+
Sbjct: 126 VRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRV 185

Query: 121 AFGKKFR--GGEIDNDNIENVIRRAVVVLKSFSATDFFP-RVGWIIDWLSGFHTRMEKSF 180
           A G+ F   G  ID D IE ++  +   L +F+ +DFFP  +G  +DWL   H ++ K F
Sbjct: 186 ALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVF 245

Query: 181 TELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAG 240
            ELD ++QHV+D H+K       D    I+D++ K+   D  K + D +KA++MD+ LAG
Sbjct: 246 KELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAG 305

Query: 241 VETGANTLVWALTELVKNPKVMKKLQDEIRSC--IKEDRVKESDLEKLKYLKVVMKEVLR 300
           ++T A T++WA+TEL++NP+VMKK Q+ IR+   +K++R+ E DL K++YL  ++KE  R
Sbjct: 306 IDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   P ++PRETMSH K+ GYDI PKT + +NVW IGRDPK W +PEEF PERF  S +
Sbjct: 366 LHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSSV 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D++  +F+LL FGSGRRICP M MA   +EL L N+L  FDW +P   K +D+ +EE  +
Sbjct: 426 DFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGN 485

Query: 421 FVVAKKSPLQLVPI 430
             + KK PLQLVP+
Sbjct: 486 ISIVKKIPLQLVPV 499

[Close]

BLAST of Lsi04G006220 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 423.7 bits (1088), Expect = 1.8e-118
Identity = 214/436 (49.08%), Postives = 291/436 (66.74%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+V+SSS  AK++LK+NDL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+A+RV S Q I++EE+  L+ S ++S+S  + +NLSEKF   T+++  + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AF   F    ++ND  + +I  A + L SFSA++FFP  GWIIDWL+G   R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D ++Q + D H +  K       E+ VD+LLK  +E+      K   + +KA+LM++LL 
Sbjct: 246 DVFYQQMFDLHKQENK----QGVEDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRS-CIKEDRVKESDLEKLKYLKVVMKEVLR 300
            + T A T+ WA+ EL++NP+VMKK+Q EIR+  I +  +   D++ L YLK+V+KE  R
Sbjct: 306 AINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPRE MS F++NGY I PKTL+Y+NVWAIGRDP  W + + F PERF+ + I
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRRICP M M T M+E  LANML  FDW++P  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICY 432
             V KK+ L LVP+ Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

[Close]

BLAST of Lsi04G006220 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 414.5 bits (1064), Expect = 1.1e-115
Identity = 214/438 (48.86%), Postives = 294/438 (67.12%), Query Frame = 0


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLG VPTL++SSS  AK+ L+  DL CCSRP L  G   SY  LD+    Y ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+C   LF+A ++QS Q I+ EE+  +++SI++SSS    +NLS+ F + T ++  + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F G  +++D    ++R    +L SFSA+DF P VGWIID  +G     +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H K  K  G +D   +VDVLL+  +E++     K   + IKA+LM+ILL 
Sbjct: 246 DAFYEQIFDLH-KEEKEVGSED---LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIK-EDRVKESDLEKLKYLKVVMKEVLR 300
           G++T A T+ WA+ EL KNP+VMKK+Q EIR+ IK ++R+   D +KL+YLK+V+KE  R
Sbjct: 306 GIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPR+ ++ F++NGY I  KT +++NVWAIGRDP  W +PE FLPERF  S I
Sbjct: 366 LHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELLSFGSGRRICP + M T M+E  LANML  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
             V+KKS L LVP+ Y +
Sbjct: 486 LTVSKKSELVLVPVKYLD 499

The following BLAST results are available for this feature:

BLAST of Lsi04G006220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIP4	9.2e-120	48.40	Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1	[more]
Q9LIP3	3.0e-118	49.09	Cytochrome P450 71B37 OS=Arabidopsis thaliana OX=3702 GN=CYP71B37 PE=3 SV=2	[more]
O65788	1.1e-117	46.77	Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2	[more]
O64718	2.5e-117	49.08	Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3	[more]
Q9LTL0	1.5e-114	48.86	Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1	[more]

BLAST of Lsi04G006220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CWC3	1.6e-167	67.49	Cytochrome P450 71B37-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S4DW34	1.6e-167	67.49	cytochrome P450 71B37-like OS=Cucumis melo OX=3656 GN=LOC103488948 PE=3 SV=1	[more]
A0A6J1DCP0	3.8e-161	65.75	cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1DEX7	3.8e-161	65.75	cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1GWP7	1.5e-160	65.83	cytochrome P450 71B26-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458...	[more]

BLAST of Lsi04G006220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892966.1	5.8e-180	73.64	cytochrome P450 71B37-like [Benincasa hispida]	[more]
KAA0034250.1	3.3e-167	67.49	cytochrome P450 71B37-like [Cucumis melo var. makuwa] >TYK15670.1 cytochrome P45...	[more]
XP_016900197.1	3.3e-167	67.49	PREDICTED: cytochrome P450 71B37-like [Cucumis melo]	[more]
XP_004135500.2	4.3e-167	67.19	cytochrome P450 71B37-like [Cucumis sativus] >KAE8648691.1 hypothetical protein ...	[more]
XP_022151972.1	7.9e-161	65.75	cytochrome P450 71B34-like isoform X1 [Momordica charantia] >XP_022151976.1 cyto...	[more]

BLAST of Lsi04G006220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26320.1	6.5e-121	48.40	cytochrome P450, family 71, subfamily B, polypeptide 36	[more]
AT3G26330.1	2.1e-119	49.09	cytochrome P450, family 71, subfamily B, polypeptide 37	[more]
AT1G13080.1	8.0e-119	46.77	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT2G02580.1	1.8e-118	49.08	cytochrome P450, family 71, subfamily B, polypeptide 9	[more]
AT3G26290.1	1.1e-115	48.86	cytochrome P450, family 71, subfamily B, polypeptide 26	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 225..242 score: 29.98 coord: 364..374 score: 50.56 coord: 374..397 score: 24.91 coord: 112..130 score: 22.29 coord: 18..39 score: 23.84 coord: 245..271 score: 35.71 coord: 287..305 score: 35.91 coord: 328..352 score: 40.03
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 288..299 score: 36.25 coord: 365..374 score: 53.41 coord: 236..253 score: 40.31
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..408 e-value: 1.9E-80 score: 270.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..427 e-value: 2.4E-101 score: 341.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..427
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 1..431
None	No IPR available	PANTHER	PTHR47956:SF5	CYTOCHROME P450 71B16-RELATED	coord: 1..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G006220.1	Lsi04G006220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen