Lsi04G005770 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G005770
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAP-1 complex subunit mu-2-like
Locationchr04: 5266171 .. 5270366 (-)
RNA-Seq ExpressionLsi04G005770
SyntenyLsi04G005770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAATATGAATTTATTTATCAGAAAAGGGAAATGAAAGGGAAAACTCCCAATTGCTAACTAAAATAACTCGGCCATTGGAACAGCTTGCCGCAGATCGTCCTCTAGTAATGGTTTGAGCGTCGGCATCCAGAGGACACAAGTTTCTGGCTCGACGATGTGTCGAATTGATTCAACTAGATCTACCCTTCTACCCAGTTGATTCTTCTGCTGAACATCGCGAGATCTCCTTCAATTTCTTATGTTCTCCTTGATTTCGTTAACAACCTAGCTAGCCGGAGGAAGAATTGTTTCTACTAGTGATTTACAGACCGCTTTCTGATTTGATTCAATGGCGGGGGCGGCCTCCGCGCTGTTCCTATTAGACATCAAAGGGCGTGTTCTCATTTGGCGCGACTATCGTGGCGATGTCTCAGCAGTTCAGGCCGAGCGCTTCTTCACCAAGCTCATCGAGAAGGAGGTTCTGGATCTTTGTTTTATTTAATCAATCCCTGATAGAATCGTACTCTTATTCGCTTTTTAGGACGAGTGCTTTAGTTTTTGTTCACTAGTAAATTGATGATATATGCGACTGAATCAGAGAAATTGGAGGTCCTCGTTTCGGATTTGTTCTTTTATGTCATTAGGAAGCAGATTCAAATAAAGAACTGGCTAAATAAAATGATTTGAATCTCTACCTGACAGATTCTTCAAAATCTTTTGATGTCAGGGTGATCCGCAATCGCAAGATCCAGTAGTTTACGATAATGGCATAAGCTACATGTTCATTCAGCACAATAATGTGTACCTGATGGTTGCTGCGAGGCAGAATTGCAATGCTGCAAGTCTTCTTTCCTTTTTGCATCGAGTAGTTGATGTAAGTTGTATATATGATCATAGCGTTTTCCAACAAAGCATTTTGGTATTTCAAACATGTTTAATTTGGATTAATGACAGTGTACACATTATCTTAGTTCCTGAAGTTACATGGTTCAGTAAAATTTCGGATTAGATTTCTTAATATTTGAGGCTACTTAGTATCTTCTTTCTATCAACAATAGGAGAATAATTCTTTTTCCCATTAGTTTACTTTTCTGCATTGATTTCGGTGGTAAATGGTAATGAATTTTCTATTTTGTTTTCCTTATAAAATTTAGGTAGATTTTGGGACTTCCGAGCAAATATATTTCGTGATGAGAAAGAAAAGTAATAAATGATTGACGTGAGTCATTATGCTACAGGTCTTCAAGCATTATTTTGAAGAGTTGGAAGAAGAATCGCTTAGAGACAACTTTGTTGTGGTGGTAAGATATGCCTTTCACAGATCAAATCCTTATAAGTTTCTTAAATTGTTCACCATATCATGTATTGTGATTTATGTCAGGTGGTGAATGATAATATTAATACTTATATTCTTTGTAGTATGAGTTGCTTGATGAAATCATGGACTTTGGGTATCCTCAATACACCGAGGCCAAAATTCTTAGTGAGTTTATAAAGACTGATGCTTATAGAATGGAGGTCGCTCAGAGGCCACCCATGGCAGTAACAAATGCAGTTTCTTGGCGGAGTGAAGGGATAAATTACAAGAAGAATGAGGTATGTGTTCCTTGAAAGAATCAGGTGGATTTGTTCATTTGTAATTTTTAAACCTTAGTAGGTAATGTCCTGTTCTTATTTCTCCAGGTCTTTTTGGATGTGGTGGAGAGTGTTAATATACTTGTCAACAGCAACGGGCAAATAATTAGGTCAGATGTTGTGGGCGCATTGAAGATGAGAACTTACTTAAGGTTTGCTCACAATGCTTTGAGAAATTTATGTCTTGACACGGAAAAAGCTTGTACTTTCTTCACTTGCATATTGCACAATAATATCCTCAAAAGTACTTTTCCTGGTAATCAAGAAAAGTTTTTTAGTCATGCATACATCCTATTATTGGTTTGCTCTCTCTTTTACTTTCACATAAGAAACGATTTTAAATTGTTTTAGCAAACTCTAAACAGGGAAACTAAACCATTCAGAAAGTATGATCTATTTAATGATTCTAGCAAGCTTAGAAGAAGTGTTAACTTTTGAAACATGTGAATATTGTCTAAGTGGTATTGTTGAAATCTTCATTTCTTTGCTTTCTGTTTTCGTATTTCATTGCTTTATTATTATATGAATTCGTACTTATTCGTAACTTGGCAATGTGGTATTGTTGAAACTTTCATTTCCTTGCTTTCCAGTCTCCTGTCAATGATTTTTTATAATATCCTTCTCATTCCATTTGTATCTTTGCTAGTCGACAAGCAGAAATTAAGGTTTGGTTTTATCTGTTGTTGTAGTGGTATGCCTGAGTGTAAGCTTGGGCTCAATGATAGAGTATTATTGGAAGCACAAGGCCGAACCACAAAAGGGAAAGCTATTGATTTGGAAGATATCAAATTTCACCAGTATGTATCAAACCATATGGTGCATTTATTTTCCTGTACTAGTAATATCTGTGTATTGTGTTGGGCACATAAGCTAATACAATAATGTAGACTTCATAAGCATTATGCCAGTTTCTATGTTATCTAAGTATACATATGCTTTTTTTTTTTTGTGGGGCTTTAGGTGCGTACGCTTGGCCCGCTTTGAAAATGATCGGACCATATCCTTCATACCTCCAGATGGATCTTTTGATCTCATGACTTATAGACTAAGCACTCAGGTTAAATATATCCACGTAGATGCTATTTTCTAAATCACCGTTGGTTTATTCATTACTTCCTTCTATCTCGTTATTTGATGTGCTTCATCCTTTTTGTTGACAGGTAAAACCTCTTATTTGGGTAGAAGCTCAAGTTGAAAGGCATTCAAAAAGTCGTATTGAGATTATGGTGAAAGCAAGGAGCCAGTTCAAGGAGCGAAGGTATTATAAAATGTCTTTTTATTTTTTTTGAGGGGGGTCAGTAAAAATCTTATCTTCCTAGTTTCGTTTTACTAATAAAAGGTTCATCATGCAGCACCGCAACCAATGTTGAGATTGAGCTGCCTGTGCCAGCAGATGCTACCAACCCGAATGTTCGGACCTCAATGGGGTCTGCTTCTTATGCACCAGAAAATGATGCATTATGCTGGAAAATAAGATCGTTTCCAGGTGGCAAGGTATTTTAAATTTTATTTGCTTCTGTCAATGATACCTTACCTTATGCATGAATAGCGGATGATTCAGGAAGCTAATTTTCTTGATCTTAATCTGGAAAATTCAGGAGTATATGTTAAGGGCAGAGTTTCGACTTCCTTCAATTACATCTGAAGAAGCAACTCCAGAGAGAAAAGCGCCAATTCGTGTAAAGTTCGAGATACCATACTTCACTGTTTCTGGAATCCAGGTACACTTCCATATGCTTGCTCTGGTTATTCCTGTTTACCATTTAGTGTCTTATGAAAAGTTGGAGTATTTTTCTTGTAGTAAACCGACCTCATCTTGGATTTATTCTGTTATTTGTGATATCGGATATGTGAACACAGTTAGATCTGGTAGACTTACATTAGTTTCAGGCTCAAATATAAAAGCAAAAATCTGTTCTTTTTGTTTGTTACATCGTATTGGTGGAGAATAAGGATAACAGGGTGAATTATATGACCATTTTGTATTTGCATAAGTATAAACTTCGGAAGATATGATGTTCCTCTGTATCAGTATGAAACTAGGAGTGCAGATATGATATTTTAGATGTTAATGTGGAATACTATGATTTCGTAGGTTCGTTACCTGAAGATTATTGAGAAAAGTGGGTACCAGGCTCTTCCGTGGGTGAGATACATCACAATGGCTGGAGAGTATGAACTAAGGCTCATATGAAAATTACTTACTTTGATTTATAGACATTTCTGCAGAGTTCATATGTCGTGAATTATTCAGTTAATAATCACAGGATGAGTTAAGATTTGTTGTTGTAGAGGATTTGGTGGGGAAAAGGAAAAAAAAAAAAAAAAAAATCTGTTTTCTACTCTTCTTTTGTTTGTGAAGCATATTGCAGCTTTTGTAAGTCCATAGTAACTGAGAAGGGTTTCTTCCTTTTTTTTTTCTTTTTTTCTTTCTTACTATACTAGGTGGTCGGGTTGAATTCTATTTTGCTCATATATTGCTTTCAACTAGCACCATTTTTATCTGTTTAATTTGTTATATAACCATTGCCATTTTCCAAATGCTCACTCTTGAATTTTTTATGAACACAAATGAACCAA

mRNA sequence

CTGAATATGAATTTATTTATCAGAAAAGGGAAATGAAAGGGAAAACTCCCAATTGCTAACTAAAATAACTCGGCCATTGGAACAGCTTGCCGCAGATCGTCCTCTAGTAATGGTTTGAGCGTCGGCATCCAGAGGACACAAGTTTCTGGCTCGACGATGTGTCGAATTGATTCAACTAGATCTACCCTTCTACCCAGTTGATTCTTCTGCTGAACATCGCGAGATCTCCTTCAATTTCTTATGTTCTCCTTGATTTCGTTAACAACCTAGCTAGCCGGAGGAAGAATTGTTTCTACTAGTGATTTACAGACCGCTTTCTGATTTGATTCAATGGCGGGGGCGGCCTCCGCGCTGTTCCTATTAGACATCAAAGGGCGTGTTCTCATTTGGCGCGACTATCGTGGCGATGTCTCAGCAGTTCAGGCCGAGCGCTTCTTCACCAAGCTCATCGAGAAGGAGGGTGATCCGCAATCGCAAGATCCAGTAGTTTACGATAATGGCATAAGCTACATGTTCATTCAGCACAATAATGTGTACCTGATGGTTGCTGCGAGGCAGAATTGCAATGCTGCAAGTCTTCTTTCCTTTTTGCATCGAGTAGTTGATGTCTTCAAGCATTATTTTGAAGAGTTGGAAGAAGAATCGCTTAGAGACAACTTTGTTGTGGTGTATGAGTTGCTTGATGAAATCATGGACTTTGGGTATCCTCAATACACCGAGGCCAAAATTCTTAGTGAGTTTATAAAGACTGATGCTTATAGAATGGAGGTCGCTCAGAGGCCACCCATGGCAGTAACAAATGCAGTTTCTTGGCGGAGTGAAGGGATAAATTACAAGAAGAATGAGGTCTTTTTGGATGTGGTGGAGAGTGTTAATATACTTGTCAACAGCAACGGGCAAATAATTAGGTCAGATGTTGTGGGCGCATTGAAGATGAGAACTTACTTAAGTGGTATGCCTGAGTGTAAGCTTGGGCTCAATGATAGAGTATTATTGGAAGCACAAGGCCGAACCACAAAAGGGAAAGCTATTGATTTGGAAGATATCAAATTTCACCAGTGCGTACGCTTGGCCCGCTTTGAAAATGATCGGACCATATCCTTCATACCTCCAGATGGATCTTTTGATCTCATGACTTATAGACTAAGCACTCAGGTAAAACCTCTTATTTGGGTAGAAGCTCAAGTTGAAAGGCATTCAAAAAGTCGTATTGAGATTATGGTGAAAGCAAGGAGCCAGTTCAAGGAGCGAAGGTATTATAAAATCACCGCAACCAATGTTGAGATTGAGCTGCCTGTGCCAGCAGATGCTACCAACCCGAATGTTCGGACCTCAATGGGGTCTGCTTCTTATGCACCAGAAAATGATGCATTATGCTGGAAAATAAGATCGTTTCCAGGTGGCAAGGAGTATATGTTAAGGGCAGAGTTTCGACTTCCTTCAATTACATCTGAAGAAGCAACTCCAGAGAGAAAAGCGCCAATTCGTGTAAAGTTCGAGATACCATACTTCACTGTTTCTGGAATCCAGGTTCGTTACCTGAAGATTATTGAGAAAAGTGGGTACCAGGCTCTTCCGTGGGTGAGATACATCACAATGGCTGGAGAGTATGAACTAAGGCTCATATGAAAATTACTTACTTTGATTTATAGACATTTCTGCAGAGTTCATATGTCGTGAATTATTCAGTTAATAATCACAGGATGAGTTAAGATTTGTTGTTGTAGAGGATTTGGTGGGGAAAAGGAAAAAAAAAAAAAAAAAAATCTGTTTTCTACTCTTCTTTTGTTTGTGAAGCATATTGCAGCTTTTGTAAGTCCATAGTAACTGAGAAGGGTTTCTTCCTTTTTTTTTTCTTTTTTTCTTTCTTACTATACTAGGTGGTCGGGTTGAATTCTATTTTGCTCATATATTGCTTTCAACTAGCACCATTTTTATCTGTTTAATTTGTTATATAACCATTGCCATTTTCCAAATGCTCACTCTTGAATTTTTTATGAACACAAATGAACCAA

Coding sequence (CDS)

ATGGCGGGGGCGGCCTCCGCGCTGTTCCTATTAGACATCAAAGGGCGTGTTCTCATTTGGCGCGACTATCGTGGCGATGTCTCAGCAGTTCAGGCCGAGCGCTTCTTCACCAAGCTCATCGAGAAGGAGGGTGATCCGCAATCGCAAGATCCAGTAGTTTACGATAATGGCATAAGCTACATGTTCATTCAGCACAATAATGTGTACCTGATGGTTGCTGCGAGGCAGAATTGCAATGCTGCAAGTCTTCTTTCCTTTTTGCATCGAGTAGTTGATGTCTTCAAGCATTATTTTGAAGAGTTGGAAGAAGAATCGCTTAGAGACAACTTTGTTGTGGTGTATGAGTTGCTTGATGAAATCATGGACTTTGGGTATCCTCAATACACCGAGGCCAAAATTCTTAGTGAGTTTATAAAGACTGATGCTTATAGAATGGAGGTCGCTCAGAGGCCACCCATGGCAGTAACAAATGCAGTTTCTTGGCGGAGTGAAGGGATAAATTACAAGAAGAATGAGGTCTTTTTGGATGTGGTGGAGAGTGTTAATATACTTGTCAACAGCAACGGGCAAATAATTAGGTCAGATGTTGTGGGCGCATTGAAGATGAGAACTTACTTAAGTGGTATGCCTGAGTGTAAGCTTGGGCTCAATGATAGAGTATTATTGGAAGCACAAGGCCGAACCACAAAAGGGAAAGCTATTGATTTGGAAGATATCAAATTTCACCAGTGCGTACGCTTGGCCCGCTTTGAAAATGATCGGACCATATCCTTCATACCTCCAGATGGATCTTTTGATCTCATGACTTATAGACTAAGCACTCAGGTAAAACCTCTTATTTGGGTAGAAGCTCAAGTTGAAAGGCATTCAAAAAGTCGTATTGAGATTATGGTGAAAGCAAGGAGCCAGTTCAAGGAGCGAAGGTATTATAAAATCACCGCAACCAATGTTGAGATTGAGCTGCCTGTGCCAGCAGATGCTACCAACCCGAATGTTCGGACCTCAATGGGGTCTGCTTCTTATGCACCAGAAAATGATGCATTATGCTGGAAAATAAGATCGTTTCCAGGTGGCAAGGAGTATATGTTAAGGGCAGAGTTTCGACTTCCTTCAATTACATCTGAAGAAGCAACTCCAGAGAGAAAAGCGCCAATTCGTGTAAAGTTCGAGATACCATACTTCACTGTTTCTGGAATCCAGGTTCGTTACCTGAAGATTATTGAGAAAAGTGGGTACCAGGCTCTTCCGTGGGTGAGATACATCACAATGGCTGGAGAGTATGAACTAAGGCTCATATGA

Protein sequence

MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISYMFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEIMDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVESVNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKARSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKEYMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITMAGEYELRLI
Homology
BLAST of Lsi04G005770 vs. ExPASy Swiss-Prot
Match: O22715 (AP-1 complex subunit mu-2 OS=Arabidopsis thaliana OX=3702 GN=AP1M2 PE=1 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 3.9e-227
Identity = 387/432 (89.58%), Postives = 412/432 (95.37%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVL+WRDYRGDVSA QAERFFTKLIEKEGD QS DPV YDNG++Y
Sbjct: 1   MAGAASALFLLDIKGRVLVWRDYRGDVSAAQAERFFTKLIEKEGDSQSNDPVAYDNGVTY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MF+QH+NVYLM+A+RQNCNAASLL FLHRVVDVFKHYFEELEEESLRDNFVVVYELLDE+
Sbjct: 61  MFVQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEM 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEA+ILSEFIKTDAYRMEV QRPPMAVTNAVSWRSEGI YKKNEVFLDV+E+
Sbjct: 121 MDFGYPQYTEARILSEFIKTDAYRMEVTQRPPMAVTNAVSWRSEGIQYKKNEVFLDVIEN 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQI+RSDVVGALKMRTYL+GMPECKLGLNDRVLLEAQGR TKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIVRSDVVGALKMRTYLTGMPECKLGLNDRVLLEAQGRATKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQ+E HS+SR+E+++KA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIESHSRSRVEMLIKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVP DA+NP VRTS+GSASYAPE DAL WKI+SFPG KE
Sbjct: 301 RSQFKERS----TATNVEIELPVPTDASNPTVRTSLGSASYAPEKDALVWKIKSFPGNKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEF LPSIT+EEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFHLPSITAEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLV 428

BLAST of Lsi04G005770 vs. ExPASy Swiss-Prot
Match: Q9SAC9 (AP-1 complex subunit mu-1 OS=Arabidopsis thaliana OX=3702 GN=AP1M1 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 2.3e-216
Identity = 369/432 (85.42%), Postives = 403/432 (93.29%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVL+WRDYRGDV+A QAERFFTKLIE EGD QS DPV YDNG++Y
Sbjct: 1   MAGAASALFLLDIKGRVLVWRDYRGDVTAAQAERFFTKLIETEGDSQSNDPVAYDNGVTY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MF+QH+N+YLM+A+RQNCNAASLL FLHRVVDVFKHYFEELEEESLRDNFVVVYELLDE+
Sbjct: 61  MFVQHSNIYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEM 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQ+TEA+ILSEFIKTDAYRMEV QRPPMAVTN+VSWRSEG+ +KKNEVFLDV+ES
Sbjct: 121 MDFGYPQFTEARILSEFIKTDAYRMEVTQRPPMAVTNSVSWRSEGLKFKKNEVFLDVIES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQI+RSDVVGALKMRTYLSGMPECKLGLNDR+LLEAQGR  KGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIVRSDVVGALKMRTYLSGMPECKLGLNDRILLEAQGRAIKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEA +ERHS+SR+E++VKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAHIERHSRSRVEMLVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFK+R Y    AT+VEIELPVP DA NP+VRTS+GSA+YAPE DAL WKI+ F G KE
Sbjct: 301 RSQFKDRSY----ATSVEIELPVPTDAYNPDVRTSLGSAAYAPEKDALVWKIQYFYGNKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           + L+A+F LPSI +EEATPERKAPIRVKFEIP F VSGIQVRYLKIIEKSGYQA PWVRY
Sbjct: 361 HTLKADFHLPSIAAEEATPERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSGYQAHPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLM 428

BLAST of Lsi04G005770 vs. ExPASy Swiss-Prot
Match: Q2KJ81 (AP-1 complex subunit mu-1 OS=Bos taurus OX=9913 GN=AP1M1 PE=1 SV=3)

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-154
Identity = 262/428 (61.21%), Postives = 343/428 (80.14%), Query Frame = 0

Query: 4   AASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISYMFI 63
           +ASA+++LD+KG+VLI R+YRGDV   + E F   L+EKE +     P++   G+ +M+I
Sbjct: 2   SASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKE-EEGMLSPILAHGGVRFMWI 61

Query: 64  QHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEIMDF 123
           +HNN+YL+  +++N   + + SFL++VV VF  YF+ELEEES+RDNFV++YELLDE+MDF
Sbjct: 62  KHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDF 121

Query: 124 GYPQYTEAKILSEFIKTDAYRMEV-AQRPPMAVTNAVSWRSEGINYKKNEVFLDVVESVN 183
           GYPQ T++KIL E+I  + +++E  A RPP  VTNAVSWRSEGI Y+KNEVFLDV+ESVN
Sbjct: 122 GYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVN 181

Query: 184 ILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIKFH 243
           +LV++NG ++RS++VG++KMR +LSGMPE +LGLND+VL +  GR  K K+++LED+KFH
Sbjct: 182 LLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGR-GKSKSVELEDVKFH 241

Query: 244 QCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKARS 303
           QCVRL+RFENDRTISFIPPDG F+LM+YRL+T VKPLIW+E+ +E+HS SRIE M+KA+S
Sbjct: 242 QCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKS 301

Query: 304 QFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKEYM 363
           QFK R     TA NVEI +PVP DA +P  +T++GS  + PEN  + W I+SFPGGKEY+
Sbjct: 302 QFKRRS----TANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYL 361

Query: 364 LRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYIT 423
           +RA F LPS+ +E+   E K PI VKFEIPYFT SGIQVRYLKIIEKSGYQALPWVRYIT
Sbjct: 362 MRAHFGLPSVEAED--KEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYIT 421

Query: 424 MAGEYELR 431
             G+Y+LR
Sbjct: 422 QNGDYQLR 421

BLAST of Lsi04G005770 vs. ExPASy Swiss-Prot
Match: Q9BXS5 (AP-1 complex subunit mu-1 OS=Homo sapiens OX=9606 GN=AP1M1 PE=1 SV=3)

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-154
Identity = 262/428 (61.21%), Postives = 343/428 (80.14%), Query Frame = 0

Query: 4   AASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISYMFI 63
           +ASA+++LD+KG+VLI R+YRGDV   + E F   L+EKE +     P++   G+ +M+I
Sbjct: 2   SASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKE-EEGMLSPILAHGGVRFMWI 61

Query: 64  QHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEIMDF 123
           +HNN+YL+  +++N   + + SFL++VV VF  YF+ELEEES+RDNFV++YELLDE+MDF
Sbjct: 62  KHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDF 121

Query: 124 GYPQYTEAKILSEFIKTDAYRMEV-AQRPPMAVTNAVSWRSEGINYKKNEVFLDVVESVN 183
           GYPQ T++KIL E+I  + +++E  A RPP  VTNAVSWRSEGI Y+KNEVFLDV+ESVN
Sbjct: 122 GYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVN 181

Query: 184 ILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIKFH 243
           +LV++NG ++RS++VG++KMR +LSGMPE +LGLND+VL +  GR  K K+++LED+KFH
Sbjct: 182 LLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGR-GKSKSVELEDVKFH 241

Query: 244 QCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKARS 303
           QCVRL+RFENDRTISFIPPDG F+LM+YRL+T VKPLIW+E+ +E+HS SRIE M+KA+S
Sbjct: 242 QCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKS 301

Query: 304 QFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKEYM 363
           QFK R     TA NVEI +PVP DA +P  +T++GS  + PEN  + W I+SFPGGKEY+
Sbjct: 302 QFKRRS----TANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYL 361

Query: 364 LRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYIT 423
           +RA F LPS+ +E+   E K PI VKFEIPYFT SGIQVRYLKIIEKSGYQALPWVRYIT
Sbjct: 362 MRAHFGLPSVEAED--KEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYIT 421

Query: 424 MAGEYELR 431
             G+Y+LR
Sbjct: 422 QNGDYQLR 421

BLAST of Lsi04G005770 vs. ExPASy Swiss-Prot
Match: P35602 (AP-1 complex subunit mu-1-I OS=Caenorhabditis elegans OX=6239 GN=unc-101 PE=1 SV=2)

HSP 1 Score: 540.0 bits (1390), Expect = 2.4e-152
Identity = 258/428 (60.28%), Postives = 338/428 (78.97%), Query Frame = 0

Query: 4   AASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISYMFI 63
           A SA+F+LD+KG+ +I R+YRGD+     ++F   L+EKE +  S  PV+     +++FI
Sbjct: 2   ATSAMFILDLKGKTIISRNYRGDIDMTAIDKFIHLLMEKE-EEGSAAPVLTYQDTNFVFI 61

Query: 64  QHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEIMDF 123
           +H N+YL+ A R N N   +LSFL++ V+VF  YF+++EEES+RDNFVV+YELLDE+MDF
Sbjct: 62  KHTNIYLVSACRSNVNVTMILSFLYKCVEVFSEYFKDVEEESVRDNFVVIYELLDEMMDF 121

Query: 124 GYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVESVNI 183
           G+PQ TE++IL E+I  +  ++  A RPPMAVTNAVSWRSEGI Y+KNEVFLDV+ESVN+
Sbjct: 122 GFPQTTESRILQEYITQEGQKLISAPRPPMAVTNAVSWRSEGIKYRKNEVFLDVIESVNM 181

Query: 184 LVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIKFHQ 243
           L ++NG +++S++VG++KMR YL+GMPE +LGLND+VL E  GR  K K+++LED+KFHQ
Sbjct: 182 LASANGTVLQSEIVGSVKMRVYLTGMPELRLGLNDKVLFEGSGR-GKSKSVELEDVKFHQ 241

Query: 244 CVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKARSQ 303
           CVRL+RF+ DRTISFIPPDG+F+LM+YRL+T VKPLIW+E  +ERHS SR+  ++KA+SQ
Sbjct: 242 CVRLSRFDTDRTISFIPPDGAFELMSYRLTTVVKPLIWIETSIERHSHSRVSFIIKAKSQ 301

Query: 304 FKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKEYML 363
           FK R     TA NVEI +PVP+DA +P  +TS+GS  Y PE  A  W I++FPGGKEY+L
Sbjct: 302 FKRRS----TANNVEIIIPVPSDADSPKFKTSIGSVKYTPEQSAFVWTIKNFPGGKEYLL 361

Query: 364 RAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITM 423
            A   LPS+ SEE+  E + PI+VKFEIPYFT SGIQVRYLKIIEKSGYQALPWVRYIT 
Sbjct: 362 TAHLSLPSVMSEES--EGRPPIKVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQ 421

Query: 424 AGEYELRL 432
            GEYE+R+
Sbjct: 422 NGEYEMRM 421

BLAST of Lsi04G005770 vs. ExPASy TrEMBL
Match: A0A6J1HTL5 (AP-1 complex subunit mu-2-like OS=Cucurbita maxima OX=3661 GN=LOC111466478 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.1e-240
Identity = 425/432 (98.38%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPS+TSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSVTSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. ExPASy TrEMBL
Match: A0A6J1IIB8 (AP-1 complex subunit mu-2 OS=Cucurbita maxima OX=3661 GN=LOC111477743 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.4e-240
Identity = 424/432 (98.15%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQ+ERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLV 428

BLAST of Lsi04G005770 vs. ExPASy TrEMBL
Match: A0A6J1G0D9 (AP-1 complex subunit mu-2-like OS=Cucurbita moschata OX=3662 GN=LOC111449546 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.4e-240
Identity = 424/432 (98.15%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHR+VDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRLVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPS+TSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSVTSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. ExPASy TrEMBL
Match: A0A5A7SYD0 (AP-1 complex subunit mu-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001290 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.2e-240
Identity = 425/432 (98.38%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPE+DALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPEHDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. ExPASy TrEMBL
Match: A0A0A0KTL2 (MHD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G599290 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.2e-240
Identity = 425/432 (98.38%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYD+GISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDSGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. NCBI nr
Match: XP_038892524.1 (AP-1 complex subunit mu-2 [Benincasa hispida])

HSP 1 Score: 843.6 bits (2178), Expect = 7.7e-241
Identity = 427/432 (98.84%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. NCBI nr
Match: XP_022966913.1 (AP-1 complex subunit mu-2-like [Cucurbita maxima])

HSP 1 Score: 842.0 bits (2174), Expect = 2.2e-240
Identity = 425/432 (98.38%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPS+TSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSVTSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. NCBI nr
Match: XP_022977397.1 (AP-1 complex subunit mu-2 [Cucurbita maxima] >XP_023544774.1 AP-1 complex subunit mu-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 841.6 bits (2173), Expect = 2.9e-240
Identity = 424/432 (98.15%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQ+ERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLV 428

BLAST of Lsi04G005770 vs. NCBI nr
Match: XP_022945245.1 (AP-1 complex subunit mu-2-like [Cucurbita moschata] >XP_023542291.1 AP-1 complex subunit mu-2-like [Cucurbita pepo subsp. pepo] >KAG6573718.1 AP-1 complex subunit mu-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012794.1 AP-1 complex subunit mu-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.9 bits (2171), Expect = 5.0e-240
Identity = 424/432 (98.15%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHR+VDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRLVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPS+TSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSVTSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRLI
Sbjct: 421 ITMAGEYELRLI 428

BLAST of Lsi04G005770 vs. NCBI nr
Match: XP_022957314.1 (AP-1 complex subunit mu-2 [Cucurbita moschata] >KAG6601524.1 AP-1 complex subunit mu-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032303.1 AP-1 complex subunit mu-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.5 bits (2170), Expect = 6.5e-240
Identity = 423/432 (97.92%), Postives = 427/432 (98.84%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY
Sbjct: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MFIQHNNVYLMVAARQNCNAASLLSFLHR+VDVFKHYFEELEEESLRDNFVVVYELLDEI
Sbjct: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRLVDVFKHYFEELEEESLRDNFVVVYELLDEI 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES
Sbjct: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQ+ERHSKSRIEIMVKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIERHSKSRIEIMVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE
Sbjct: 301 RSQFKERS----TATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLV 428

BLAST of Lsi04G005770 vs. TAIR 10
Match: AT1G60780.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 788.5 bits (2035), Expect = 2.7e-228
Identity = 387/432 (89.58%), Postives = 412/432 (95.37%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVL+WRDYRGDVSA QAERFFTKLIEKEGD QS DPV YDNG++Y
Sbjct: 1   MAGAASALFLLDIKGRVLVWRDYRGDVSAAQAERFFTKLIEKEGDSQSNDPVAYDNGVTY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MF+QH+NVYLM+A+RQNCNAASLL FLHRVVDVFKHYFEELEEESLRDNFVVVYELLDE+
Sbjct: 61  MFVQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEM 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQYTEA+ILSEFIKTDAYRMEV QRPPMAVTNAVSWRSEGI YKKNEVFLDV+E+
Sbjct: 121 MDFGYPQYTEARILSEFIKTDAYRMEVTQRPPMAVTNAVSWRSEGIQYKKNEVFLDVIEN 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQI+RSDVVGALKMRTYL+GMPECKLGLNDRVLLEAQGR TKGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIVRSDVVGALKMRTYLTGMPECKLGLNDRVLLEAQGRATKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDG+FDLMTYRLSTQVKPLIWVEAQ+E HS+SR+E+++KA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIESHSRSRVEMLIKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFKER     TATNVEIELPVP DA+NP VRTS+GSASYAPE DAL WKI+SFPG KE
Sbjct: 301 RSQFKERS----TATNVEIELPVPTDASNPTVRTSLGSASYAPEKDALVWKIKSFPGNKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           YMLRAEF LPSIT+EEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY
Sbjct: 361 YMLRAEFHLPSITAEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLV 428

BLAST of Lsi04G005770 vs. TAIR 10
Match: AT1G10730.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 752.7 bits (1942), Expect = 1.7e-217
Identity = 369/432 (85.42%), Postives = 403/432 (93.29%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           MAGAASALFLLDIKGRVL+WRDYRGDV+A QAERFFTKLIE EGD QS DPV YDNG++Y
Sbjct: 1   MAGAASALFLLDIKGRVLVWRDYRGDVTAAQAERFFTKLIETEGDSQSNDPVAYDNGVTY 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEI 120
           MF+QH+N+YLM+A+RQNCNAASLL FLHRVVDVFKHYFEELEEESLRDNFVVVYELLDE+
Sbjct: 61  MFVQHSNIYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEM 120

Query: 121 MDFGYPQYTEAKILSEFIKTDAYRMEVAQRPPMAVTNAVSWRSEGINYKKNEVFLDVVES 180
           MDFGYPQ+TEA+ILSEFIKTDAYRMEV QRPPMAVTN+VSWRSEG+ +KKNEVFLDV+ES
Sbjct: 121 MDFGYPQFTEARILSEFIKTDAYRMEVTQRPPMAVTNSVSWRSEGLKFKKNEVFLDVIES 180

Query: 181 VNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRTTKGKAIDLEDIK 240
           VNILVNSNGQI+RSDVVGALKMRTYLSGMPECKLGLNDR+LLEAQGR  KGKAIDLEDIK
Sbjct: 181 VNILVNSNGQIVRSDVVGALKMRTYLSGMPECKLGLNDRILLEAQGRAIKGKAIDLEDIK 240

Query: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQVERHSKSRIEIMVKA 300
           FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEA +ERHS+SR+E++VKA
Sbjct: 241 FHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAHIERHSRSRVEMLVKA 300

Query: 301 RSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPENDALCWKIRSFPGGKE 360
           RSQFK+R Y    AT+VEIELPVP DA NP+VRTS+GSA+YAPE DAL WKI+ F G KE
Sbjct: 301 RSQFKDRSY----ATSVEIELPVPTDAYNPDVRTSLGSAAYAPEKDALVWKIQYFYGNKE 360

Query: 361 YMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRY 420
           + L+A+F LPSI +EEATPERKAPIRVKFEIP F VSGIQVRYLKIIEKSGYQA PWVRY
Sbjct: 361 HTLKADFHLPSIAAEEATPERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSGYQAHPWVRY 420

Query: 421 ITMAGEYELRLI 433
           ITMAGEYELRL+
Sbjct: 421 ITMAGEYELRLM 428

BLAST of Lsi04G005770 vs. TAIR 10
Match: AT5G46630.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 357.8 bits (917), Expect = 1.2e-98
Identity = 189/444 (42.57%), Postives = 274/444 (61.71%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           M  AASA++ L+++G VLI R YR DV     + F T +++ +       PV    G S+
Sbjct: 1   MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTK--ELGNCPVRQIGGCSF 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYF-EELEEESLRDNFVVVYELLDE 120
           ++++ +NVY+++    N N A    F+   V +FK YF    +E+++R+NFV++YELLDE
Sbjct: 61  VYMRISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDE 120

Query: 121 IMDFGYPQYTEAKILSEFIKTDAYRMEVAQRP--------PMAVTNAVSWRSEGINYKKN 180
           IMDFGYPQ    +IL  +I  +  R   + +P         + VT AV WR EG+ YKKN
Sbjct: 121 IMDFGYPQNLSPEILKLYITQEGVRSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKN 180

Query: 181 EVFLDVVESVNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGR---- 240
           EVFLD+VESVN+L++S G ++R DV G + M+ +LSGMP+ KLGLND++ LE +      
Sbjct: 181 EVFLDIVESVNLLMSSKGNVLRCDVTGKVLMKCFLSGMPDLKLGLNDKIGLEKESEMKSR 240

Query: 241 -TTKGKAIDLEDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQV 300
               GK I+L+D+ FHQCV L RF +++T+SF+PPDG F+LM YR++  V     V   +
Sbjct: 241 PAKSGKTIELDDVTFHQCVNLTRFNSEKTVSFVPPDGEFELMKYRITEGVNLPFRVLPTI 300

Query: 301 ERHSKSRIEIMVKARSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPEND 360
           +   ++R+E+ VK +S F      K+ A  V +++PVP      N + + G A Y P  D
Sbjct: 301 KELGRTRMEVNVKVKSVFGA----KMFALGVVVKIPVPKQTAKTNFQVTTGRAKYNPSID 360

Query: 361 ALCWKIRSFPGGKEYMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKI 420
            L WKIR FPG  E  L AE  L S   E+ +  R  PI+++F++P FT SG++VR+LK+
Sbjct: 361 CLVWKIRKFPGQTESTLSAEIELISTMGEKKSWTR-PPIQMEFQVPMFTASGLRVRFLKV 420

Query: 421 IEKSGYQALPWVRYITMAGEYELR 431
            EKSGY  + WVRYIT AG YE+R
Sbjct: 421 WEKSGYNTVEWVRYITKAGSYEIR 437

BLAST of Lsi04G005770 vs. TAIR 10
Match: AT5G46630.2 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.3e-88
Identity = 173/420 (41.19%), Postives = 256/420 (60.95%), Query Frame = 0

Query: 1   MAGAASALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYDNGISY 60
           M  AASA++ L+++G VLI R YR DV     + F T +++ +       PV    G S+
Sbjct: 1   MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTK--ELGNCPVRQIGGCSF 60

Query: 61  MFIQHNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYF-EELEEESLRDNFVVVYELLDE 120
           ++++ +NVY+++    N N A    F+   V +FK YF    +E+++R+NFV++YELLDE
Sbjct: 61  VYMRISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDE 120

Query: 121 IMDFGYPQYTEAKILSEFIKTDAYRMEVAQRP--------PMAVTNAVSWRSEGINYKKN 180
           IMDFGYPQ    +IL  +I  +  R   + +P         + VT AV WR EG+ YKKN
Sbjct: 121 IMDFGYPQNLSPEILKLYITQEGVRSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKN 180

Query: 181 EVFLDVVESVNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGR---- 240
           EVFLD+VESVN+L++S G ++R DV G + M+ +LSGMP+ KLGLND++ LE +      
Sbjct: 181 EVFLDIVESVNLLMSSKGNVLRCDVTGKVLMKCFLSGMPDLKLGLNDKIGLEKESEMKSR 240

Query: 241 -TTKGKAIDLEDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQV 300
               GK I+L+D+ FHQCV L RF +++T+SF+PPDG F+LM YR++  V     V   +
Sbjct: 241 PAKSGKTIELDDVTFHQCVNLTRFNSEKTVSFVPPDGEFELMKYRITEGVNLPFRVLPTI 300

Query: 301 ERHSKSRIEIMVKARSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSASYAPEND 360
           +   ++R+E+ VK +S F      K+ A  V +++PVP      N + + G A Y P  D
Sbjct: 301 KELGRTRMEVNVKVKSVFGA----KMFALGVVVKIPVPKQTAKTNFQVTTGRAKYNPSID 360

Query: 361 ALCWKIRSFPGGKEYMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSGIQVRYLKI 407
            L WKIR FPG  E  L AE  L S   E+ +  R  PI+++F++P FT SG++VR+LK+
Sbjct: 361 CLVWKIRKFPGQTESTLSAEIELISTMGEKKSWTR-PPIQMEFQVPMFTASGLRVRFLKV 413

BLAST of Lsi04G005770 vs. TAIR 10
Match: AT4G24550.2 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 258.8 bits (660), Expect = 7.5e-69
Identity = 144/454 (31.72%), Postives = 245/454 (53.96%), Query Frame = 0

Query: 6   SALFLLDIKGRVLIWRDYRGDVSAVQAERFFTKLIEKEGDPQSQDPVVYD-NGISYMFIQ 65
           S  F+L  +G  +++RDYR +V     E FF K+   + D  ++ P +++ +G++Y  ++
Sbjct: 4   SQFFVLSQRGDNIVFRDYRAEVPKGSTETFFRKVKFWKEDGNAEAPPIFNVDGVNYFHVK 63

Query: 66  HNNVYLMVAARQNCNAASLLSFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEIMDFG 125
              +Y +   R N + + +L  L R+  V K Y   L E+S R NFV+VYELLDE++DFG
Sbjct: 64  VVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELLDEVIDFG 123

Query: 126 YPQYTEAKILSEFIKTDAYRMEVAQRPPM----------------AVTNAVSWRSEGINY 185
           Y Q T  ++L  +I  +   +  A+  P+                AVT +V     G   
Sbjct: 124 YVQTTSTEVLKSYIFNEPIVVSPARLQPIDPAAIFTQGAKRMPGTAVTKSVVANDPG-GR 183

Query: 186 KKNEVFLDVVESVNILVNSNGQIIRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQG-- 245
           ++ E+F+D++E +++  +S+G I+ S++ G ++M++YLSG PE +L LN+ + +   G  
Sbjct: 184 RREEIFVDIIEKISVTFSSSGYILTSEIDGTIQMKSYLSGNPEIRLALNEDLNIGRGGRS 243

Query: 246 ----RTTKGKAIDLEDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWV 305
               R++ G  + L+D  FH+ VRL  F++DRT+S +PPDG F +M YR++ + KP   V
Sbjct: 244 VYDYRSSSGSGVILDDCNFHESVRLDSFDSDRTLSLVPPDGEFPVMNYRMTQEFKPPFHV 303

Query: 306 EAQVERHSKSRIEIMVKARSQFKERRYYKITATNVEIELPVPADATNPNVRTSMGSA--- 365
              +E   + + E+++K R++F       I A  + +++P+P   +  +     G+A   
Sbjct: 304 NTLIEEAGRLKAEVIIKIRAEFPS----DIIANTITVQMPLPNYTSRASFELEPGAAGQR 363

Query: 366 -SYAPENDALCWKIRSFPGGKEYMLRAEFRLPSITSEEATPERKAPIRVKFEIPYFTVSG 425
             +   N  L W ++   GG E+ LRA+           T E   P+ + F IP + VS 
Sbjct: 364 TDFKESNKMLEWNLKKIVGGGEHTLRAKLTFSQEFHGNITKE-AGPVSMTFTIPMYNVSK 423

Query: 426 IQVRYLKIIEK-SGYQALPWVRYITMAGEYELRL 432
           +QV+YL+I +K S Y    WVRY+T A  Y  R+
Sbjct: 424 LQVKYLQIAKKSSSYNPYRWVRYVTQANSYVARI 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O227153.9e-22789.58AP-1 complex subunit mu-2 OS=Arabidopsis thaliana OX=3702 GN=AP1M2 PE=1 SV=1[more]
Q9SAC92.3e-21685.42AP-1 complex subunit mu-1 OS=Arabidopsis thaliana OX=3702 GN=AP1M1 PE=3 SV=1[more]
Q2KJ812.0e-15461.21AP-1 complex subunit mu-1 OS=Bos taurus OX=9913 GN=AP1M1 PE=1 SV=3[more]
Q9BXS52.0e-15461.21AP-1 complex subunit mu-1 OS=Homo sapiens OX=9606 GN=AP1M1 PE=1 SV=3[more]
P356022.4e-15260.28AP-1 complex subunit mu-1-I OS=Caenorhabditis elegans OX=6239 GN=unc-101 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1HTL51.1e-24098.38AP-1 complex subunit mu-2-like OS=Cucurbita maxima OX=3661 GN=LOC111466478 PE=3 ... [more]
A0A6J1IIB81.4e-24098.15AP-1 complex subunit mu-2 OS=Cucurbita maxima OX=3661 GN=LOC111477743 PE=3 SV=1[more]
A0A6J1G0D92.4e-24098.15AP-1 complex subunit mu-2-like OS=Cucurbita moschata OX=3662 GN=LOC111449546 PE=... [more]
A0A5A7SYD03.2e-24098.38AP-1 complex subunit mu-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0KTL23.2e-24098.38MHD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G599290 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_038892524.17.7e-24198.84AP-1 complex subunit mu-2 [Benincasa hispida][more]
XP_022966913.12.2e-24098.38AP-1 complex subunit mu-2-like [Cucurbita maxima][more]
XP_022977397.12.9e-24098.15AP-1 complex subunit mu-2 [Cucurbita maxima] >XP_023544774.1 AP-1 complex subuni... [more]
XP_022945245.15.0e-24098.15AP-1 complex subunit mu-2-like [Cucurbita moschata] >XP_023542291.1 AP-1 complex... [more]
XP_022957314.16.5e-24097.92AP-1 complex subunit mu-2 [Cucurbita moschata] >KAG6601524.1 AP-1 complex subuni... [more]
Match NameE-valueIdentityDescription
AT1G60780.12.7e-22889.58Clathrin adaptor complexes medium subunit family protein [more]
AT1G10730.11.7e-21785.42Clathrin adaptor complexes medium subunit family protein [more]
AT5G46630.11.2e-9842.57Clathrin adaptor complexes medium subunit family protein [more]
AT5G46630.23.3e-8841.19Clathrin adaptor complexes medium subunit family protein [more]
AT4G24550.27.5e-6931.72Clathrin adaptor complexes medium subunit family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001392Clathrin adaptor, mu subunitPRINTSPR00314CLATHRINADPTcoord: 349..360
score: 64.39
coord: 161..189
score: 69.91
coord: 103..130
score: 74.68
coord: 309..324
score: 44.32
coord: 236..263
score: 77.27
coord: 15..35
score: 49.35
IPR001392Clathrin adaptor, mu subunitPIRSFPIRSF005992AP_complex_mucoord: 4..432
e-value: 1.1E-143
score: 477.6
NoneNo IPR availableGENE3D2.60.40.1170Mu homology domain, subdomain Bcoord: 166..426
e-value: 4.5E-103
score: 346.0
NoneNo IPR availableGENE3D3.30.450.60coord: 5..145
e-value: 2.5E-46
score: 159.0
NoneNo IPR availableGENE3D2.60.40.1170Mu homology domain, subdomain Bcoord: 278..430
e-value: 4.5E-103
score: 346.0
NoneNo IPR availablePANTHERPTHR10529:SF357ASSEMBLY PROTEIN AP47, PUTATIVE-RELATEDcoord: 1..431
NoneNo IPR availablePANTHERPTHR10529AP COMPLEX SUBUNIT MUcoord: 1..431
NoneNo IPR availableCDDcd09250AP-1_Mu1_Ctermcoord: 156..430
e-value: 0.0
score: 523.704
NoneNo IPR availableCDDcd14835AP1_Mu_Ncoord: 6..145
e-value: 1.3885E-84
score: 253.238
IPR022775AP complex, mu/sigma subunitPFAMPF01217Clat_adaptor_scoord: 7..131
e-value: 1.3E-7
score: 31.7
IPR028565Mu homology domainPFAMPF00928Adap_comp_subcoord: 159..430
e-value: 1.7E-90
score: 303.0
IPR028565Mu homology domainPROSITEPS51072MHDcoord: 170..430
score: 44.712765
IPR018240Clathrin adaptor, mu subunit, conserved sitePROSITEPS00991CLAT_ADAPTOR_M_2coord: 256..270
IPR018240Clathrin adaptor, mu subunit, conserved sitePROSITEPS00990CLAT_ADAPTOR_M_1coord: 159..179
IPR011012Longin-like domain superfamilySUPERFAMILY64356SNARE-likecoord: 6..141
IPR036168AP-2 complex subunit mu, C-terminal superfamilySUPERFAMILY49447Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptorcoord: 159..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G005770.1Lsi04G005770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0030131 clathrin adaptor complex
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0005794 Golgi apparatus