Homology
BLAST of Lsi04G005670 vs. ExPASy Swiss-Prot
Match:
Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)
HSP 1 Score: 326.6 bits (836), Expect = 4.5e-88
Identity = 187/463 (40.39%), Postives = 255/463 (55.08%), Query Frame = 0
Query: 12 LFLFLVVSLLVSSSIVRVEARKQYHSKKGKH-----------QKQKGAGNGNGSGSAPAP 71
+ + ++S ++ V AR +YH KG+H + A + S P P
Sbjct: 7 ILVIYIISTVLFGHFTNV-ARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66
Query: 72 LPD---YNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 131
PD + VF++ SFGA G+G DD+ A AWKAAC + + P F I
Sbjct: 67 SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKIT 126
Query: 132 PITLNGPCMPQLVVQIDGAVLAPPKVSTWP-KSSLFQWLNFKWLHNFTIQGSGTVDGQGF 191
+GPC P LV Q+DG ++ P WP K + QWL F L FT G GTV+G G
Sbjct: 127 STIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQ 186
Query: 192 NWWTL--STFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVK 251
WW L P PT +RF+ S N+ V+ + I NSPQ H+KFD V
Sbjct: 187 KWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVL 246
Query: 252 VDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGH 311
++ I ISSP+ SPNTDGIHL NT+ V I +S + GDDC+SI TGCS++ I + CGP H
Sbjct: 247 INEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSH 306
Query: 312 GISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKV 371
GIS+G LG S ACVSNI V+N I+++ +G+R+KTWQGG GSV N+ F NIQ+ +V
Sbjct: 307 GISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLN 366
Query: 372 PIMIDQYYCDKYRCKNQTGAVAISGITYDQIIGSYTVQ--PIHLACSSQIPCVDVDLIDI 431
I++DQYYC C+N+T AV + + Y I G+Y V+ PIH ACS + C ++ + ++
Sbjct: 367 CIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEV 426
Query: 432 QLKPSSESRGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAV 456
+L P E C N+YGK + +P ID CL G V
Sbjct: 427 ELLP-EEGELVDDPFCWNAYGKQETLTIP-PID-CLLDGSPVV 465
BLAST of Lsi04G005670 vs. ExPASy Swiss-Prot
Match:
Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)
HSP 1 Score: 295.8 bits (756), Expect = 8.5e-79
Identity = 150/374 (40.11%), Postives = 222/374 (59.36%), Query Frame = 0
Query: 71 VFNILSFGAKGNGISDDSKALLAAWKAACEIPGAT-LEIPSQYKFLIKPITLNGPCMPQL 130
+ ++ +FGAKGNG++DD+KA AWK AC T + +P Y L++PI L+GPC +L
Sbjct: 52 LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111
Query: 131 VVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKK 190
+QI G ++AP W + +WL F L T++G GTV+G G WW S + +
Sbjct: 112 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 171
Query: 191 KLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNT 250
+ PTAL F+ N+ V ++ +I+S Q H+ + V + + + +P SPNT
Sbjct: 172 PCRG----APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNT 231
Query: 251 DGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAAC 310
DGIH+ ++ + I ++ + GDDC+SI + I I +I CGPGHGIS+G LGK KS
Sbjct: 232 DGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEE 291
Query: 311 VSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYR-C 370
V +I V I +T +GVRIKTWQGG G V + F NI++++V PI+IDQYYCD + C
Sbjct: 292 VRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPC 351
Query: 371 KNQTGAVAISGITYDQIIG-SYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSL 430
NQT A++I I++ + G S + + I ++CS PC ++ L DI L+PS+ GF S
Sbjct: 352 ANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSN-GDGFTESF 411
Query: 431 CLNSYGKSQAPLLP 442
C +YG S + P
Sbjct: 412 CWEAYGSSSGQVYP 420
BLAST of Lsi04G005670 vs. ExPASy Swiss-Prot
Match:
P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)
HSP 1 Score: 290.8 bits (743), Expect = 2.7e-77
Identity = 153/396 (38.64%), Postives = 231/396 (58.33%), Query Frame = 0
Query: 71 VFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLV 130
+FN+ +GA G+G D ++A AW+AAC+ P A L +P KF++ + NGPC P
Sbjct: 58 IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117
Query: 131 VQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKK 190
++DG + A ++W + + WL F L FT+ G G +DGQG WW + N ++
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177
Query: 191 LKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTD 250
+ + D +PTA++F S + ++ + ++NSP+ HL F N VK+ I+I++P +SPNTD
Sbjct: 178 ICNDRD-RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTD 237
Query: 251 GIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACV 310
GI + +++ +Q + IG GDDCV+I TG SNI I + CGPGHGIS+G LG++ S A V
Sbjct: 238 GIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEV 297
Query: 311 SNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYC-DKYRCK 370
S + V +T +G+RIKTWQGG G ++ + N+++ + + PI+I+Q+YC C+
Sbjct: 298 SYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQ 357
Query: 371 NQTGAVAISGITYDQIIG-SYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLC 430
NQ AV I +TY I G S T I L CS +PC D+ L DI LK +S S L
Sbjct: 358 NQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGK--IASCLN 417
Query: 431 LNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDH 465
N+ G ++P C A R+ ++SH H
Sbjct: 418 DNANGYFSGHVIPA----CKNLSPSAKRKESKSHKH 444
BLAST of Lsi04G005670 vs. ExPASy Swiss-Prot
Match:
Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)
HSP 1 Score: 286.2 bits (731), Expect = 6.7e-76
Identity = 147/376 (39.10%), Postives = 221/376 (58.78%), Query Frame = 0
Query: 69 STVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQ 128
+TVFN+ +GA G+G D ++A W AAC+ A L +P+ KF + + GPC P
Sbjct: 56 ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115
Query: 129 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNK 188
L ++DG ++A P + W S + WL F L +F + G+G +DGQG WW N
Sbjct: 116 LSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNG 175
Query: 189 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 248
+ + + + +PTA++ S +VTV+++T++NSP+ HL F VK+ + I +P +SPN
Sbjct: 176 RTVCNDRN-RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPN 235
Query: 249 TDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAA 308
TDGI + ++ I+ IG GDDC++I TG SNI I + CGPGHGIS+G LG+D S A
Sbjct: 236 TDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRA 295
Query: 309 CVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYC-DKYR 368
VS++ V +T +G+RIKTWQGG G +++ N+++ + + PI+I+Q+YC
Sbjct: 296 EVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASA 355
Query: 369 CKNQTGAVAISGITYDQIIG-SYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSS 428
C+NQ AV I G+TY I G S T I L CS +PC + L ++ LK +S S
Sbjct: 356 CQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPA--SC 415
Query: 429 LCLNSYGKSQAPLLPT 443
+ N+ G L+PT
Sbjct: 416 VDKNARGFYSGRLIPT 426
BLAST of Lsi04G005670 vs. ExPASy Swiss-Prot
Match:
Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)
HSP 1 Score: 279.3 bits (713), Expect = 8.2e-74
Identity = 146/351 (41.60%), Postives = 210/351 (59.83%), Query Frame = 0
Query: 70 TVFNILSFGAKGNGISDDSKALLAAWKAACEIPGA-TLEIPSQYKFLIKPITLNGPCMPQ 129
T ++ FGAKG+G +DD++A + AWK AC GA L +P +L+K I L GPC
Sbjct: 66 TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 125
Query: 130 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQG--SGTVDGQGFNWWTLSTFYT 189
L VQI G + A K S + + +W+ F ++N ++ G +G VDG G WW S
Sbjct: 126 LTVQIFGTLSASQKRSDY--KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSC--- 185
Query: 190 NKKKLKHIPDMK-PTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPEN 249
K+ K P K PTAL FY+S ++ V+++ + N+ Q + + V+V N+ +++P +
Sbjct: 186 --KRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPAD 245
Query: 250 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 309
SPNTDGIH+ NTQ++ + S IG GDDC+SI++G N+ I+ I CGPGHGIS+G LG D
Sbjct: 246 SPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDN 305
Query: 310 SAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK 369
S A VS + V + T +GVRIKT+QGG G+ N+ F NIQ+ +VK PI+IDQ YCDK
Sbjct: 306 SKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDK 365
Query: 370 YRCKNQTGAVAISGITYDQIIG-SYTVQPIHLACSSQIPCVDVDLIDIQLK 416
+C + AV + + Y I G S + I CS PC + L + +K
Sbjct: 366 SKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK 409
BLAST of Lsi04G005670 vs. ExPASy TrEMBL
Match:
A0A1S3BEF8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1)
HSP 1 Score: 872.5 bits (2253), Expect = 8.1e-250
Identity = 423/451 (93.79%), Postives = 440/451 (97.56%), Query Frame = 0
Query: 17 VVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILS 76
+VSLLVSSS+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILS
Sbjct: 1 MVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILS 60
Query: 77 FGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGA 136
FGAKGNG+SDDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGA
Sbjct: 61 FGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGA 120
Query: 137 VLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPD 196
VLAPPKVS+WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPD
Sbjct: 121 VLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPD 180
Query: 197 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQN 256
MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQN
Sbjct: 181 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQN 240
Query: 257 TQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 316
T+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ
Sbjct: 241 TRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 300
Query: 317 NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVA 376
NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQTGAVA
Sbjct: 301 NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVA 360
Query: 377 ISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQ 436
ISGITYDQIIGSY+VQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQ+SLC NSYGKSQ
Sbjct: 361 ISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQ 420
Query: 437 APLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
APLLPTSID CLRRGGGAVRRIARSHD+VCY
Sbjct: 421 APLLPTSIDSCLRRGGGAVRRIARSHDYVCY 450
BLAST of Lsi04G005670 vs. ExPASy TrEMBL
Match:
A0A5D3CVS9 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001190 PE=3 SV=1)
HSP 1 Score: 860.1 bits (2221), Expect = 4.2e-246
Identity = 415/442 (93.89%), Postives = 431/442 (97.51%), Query Frame = 0
Query: 26 IVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILSFGAKGNGIS 85
+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILSFGAKGNG+S
Sbjct: 3 LVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVS 62
Query: 86 DDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVST 145
DDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGAVLAPPKVS+
Sbjct: 63 DDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSS 122
Query: 146 WPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPDMKPTALRFY 205
WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPDMKPTALRFY
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFY 182
Query: 206 SSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHS 265
SSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQNT+DVEIQHS
Sbjct: 183 SSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHS 242
Query: 266 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 325
NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS
Sbjct: 243 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 302
Query: 326 GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGITYDQI 385
GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQTGAVAISGITYDQI
Sbjct: 303 GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQI 362
Query: 386 IGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQAPLLPTSID 445
IGSY+VQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQ+SLC NSYGKSQAPLLPTSID
Sbjct: 363 IGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSID 422
Query: 446 YCLRRGGGAVRRIARSHDHVCY 468
CLRRGGGAVRRIARSHD+VCY
Sbjct: 423 SCLRRGGGAVRRIARSHDYVCY 443
BLAST of Lsi04G005670 vs. ExPASy TrEMBL
Match:
A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)
HSP 1 Score: 844.3 bits (2180), Expect = 2.4e-241
Identity = 409/467 (87.58%), Postives = 432/467 (92.51%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAW AAC+IPGATL+IP YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPIT 120
Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180
Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
+ST Y NKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNITI
Sbjct: 181 VSTLYNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITI 240
Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
SSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 360
LGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQ
Sbjct: 301 LGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQ 360
Query: 361 YYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSES 420
YYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS S
Sbjct: 361 YYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGS 420
Query: 421 RGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
RGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 RGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462
BLAST of Lsi04G005670 vs. ExPASy TrEMBL
Match:
A0A6J1FZF6 (polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)
HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 409/467 (87.58%), Postives = 432/467 (92.51%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120
Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180
Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
+ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITI
Sbjct: 181 VSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITI 240
Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
SSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGG 300
Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 360
LGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQ
Sbjct: 301 LGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQ 360
Query: 361 YYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSES 420
YYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS S
Sbjct: 361 YYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGS 420
Query: 421 RGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
RGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 RGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462
BLAST of Lsi04G005670 vs. ExPASy TrEMBL
Match:
A0A6J1FZB5 (polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)
HSP 1 Score: 837.4 bits (2162), Expect = 2.9e-239
Identity = 409/468 (87.39%), Postives = 432/468 (92.31%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120
Query: 121 LNGPCMPQLVV-QIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWW 180
LNGPCMPQLV+ QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180
Query: 181 TLSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
T+ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 TVSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240
Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLG 300
Query: 301 GLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMID 360
GLGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
Query: 361 QYYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSE 420
QYYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420
Query: 421 SRGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
SRGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463
BLAST of Lsi04G005670 vs. NCBI nr
Match:
XP_008446184.2 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo])
HSP 1 Score: 872.5 bits (2253), Expect = 1.7e-249
Identity = 423/451 (93.79%), Postives = 440/451 (97.56%), Query Frame = 0
Query: 17 VVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILS 76
+VSLLVSSS+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILS
Sbjct: 1 MVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILS 60
Query: 77 FGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGA 136
FGAKGNG+SDDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGA
Sbjct: 61 FGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGA 120
Query: 137 VLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPD 196
VLAPPKVS+WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPD
Sbjct: 121 VLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPD 180
Query: 197 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQN 256
MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQN
Sbjct: 181 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQN 240
Query: 257 TQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 316
T+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ
Sbjct: 241 TRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 300
Query: 317 NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVA 376
NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQTGAVA
Sbjct: 301 NISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVA 360
Query: 377 ISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQ 436
ISGITYDQIIGSY+VQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQ+SLC NSYGKSQ
Sbjct: 361 ISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQ 420
Query: 437 APLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
APLLPTSID CLRRGGGAVRRIARSHD+VCY
Sbjct: 421 APLLPTSIDSCLRRGGGAVRRIARSHDYVCY 450
BLAST of Lsi04G005670 vs. NCBI nr
Match:
TYK15622.1 (polygalacturonase [Cucumis melo var. makuwa])
HSP 1 Score: 860.1 bits (2221), Expect = 8.6e-246
Identity = 415/442 (93.89%), Postives = 431/442 (97.51%), Query Frame = 0
Query: 26 IVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILSFGAKGNGIS 85
+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILSFGAKGNG+S
Sbjct: 3 LVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVS 62
Query: 86 DDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVST 145
DDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGAVLAPPKVS+
Sbjct: 63 DDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSS 122
Query: 146 WPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPDMKPTALRFY 205
WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPDMKPTALRFY
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFY 182
Query: 206 SSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHS 265
SSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQNT+DVEIQHS
Sbjct: 183 SSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHS 242
Query: 266 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 325
NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS
Sbjct: 243 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 302
Query: 326 GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGITYDQI 385
GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQTGAVAISGITYDQI
Sbjct: 303 GVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQI 362
Query: 386 IGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQAPLLPTSID 445
IGSY+VQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQ+SLC NSYGKSQAPLLPTSID
Sbjct: 363 IGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSID 422
Query: 446 YCLRRGGGAVRRIARSHDHVCY 468
CLRRGGGAVRRIARSHD+VCY
Sbjct: 423 SCLRRGGGAVRRIARSHDYVCY 443
BLAST of Lsi04G005670 vs. NCBI nr
Match:
XP_023542214.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-241
Identity = 411/467 (88.01%), Postives = 433/467 (92.72%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQKG G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120
Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180
Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
+ST Y NKKK KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKVDNITI
Sbjct: 181 VSTLYNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITI 240
Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
SSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGG 300
Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 360
LGKDKSAACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQ
Sbjct: 301 LGKDKSAACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQ 360
Query: 361 YYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSES 420
YYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS S
Sbjct: 361 YYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGS 420
Query: 421 RGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
RGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 RGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462
BLAST of Lsi04G005670 vs. NCBI nr
Match:
XP_022966725.1 (polygalacturonase At1g48100 [Cucurbita maxima])
HSP 1 Score: 844.3 bits (2180), Expect = 4.9e-241
Identity = 409/467 (87.58%), Postives = 432/467 (92.51%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAW AAC+IPGATL+IP YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPIT 120
Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180
Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
+ST Y NKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNITI
Sbjct: 181 VSTLYNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITI 240
Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
SSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 360
LGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQ
Sbjct: 301 LGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQ 360
Query: 361 YYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSES 420
YYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS S
Sbjct: 361 YYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGS 420
Query: 421 RGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
RGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 RGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462
BLAST of Lsi04G005670 vs. NCBI nr
Match:
XP_022944899.1 (polygalacturonase At1g48100 isoform X4 [Cucurbita moschata])
HSP 1 Score: 842.0 bits (2174), Expect = 2.4e-240
Identity = 409/467 (87.58%), Postives = 432/467 (92.51%), Query Frame = 0
Query: 1 MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
M + SK LTL L VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS APAP
Sbjct: 1 MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60
Query: 61 LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61 LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120
Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180
Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
+ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITI
Sbjct: 181 VSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITI 240
Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300
SSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGG 300
Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 360
LGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQ
Sbjct: 301 LGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQ 360
Query: 361 YYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSES 420
YYCDKYRCKNQTGAVAISGI YDQIIGSYTVQPIHLACSSQIPC+DVDLI IQL PSS S
Sbjct: 361 YYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGS 420
Query: 421 RGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGAVRRIARSHDHVCY 468
RGFQS+LC NSYGKSQAPL+P+SIDYCLRRG GAVRRIARSHDH CY
Sbjct: 421 RGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462
BLAST of Lsi04G005670 vs. TAIR 10
Match:
AT3G26610.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 612.8 bits (1579), Expect = 2.2e-175
Identity = 296/469 (63.11%), Postives = 376/469 (80.17%), Query Frame = 0
Query: 6 KNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYN 65
K+L + L V+ + + S V VE RK +H KK K + ++ + N APAP P N
Sbjct: 5 KSLPILAMLLGVIIVAAAISTVSVEGRK-HHVKKIKPKHRRHSKNTPTGSPAPAPYPSTN 64
Query: 66 QTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPC 125
+ VFNI S+GAKG+G+SDDSKAL+ AWKAAC++ G +EIP+ +FL+K +TL GPC
Sbjct: 65 E---GVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPC 124
Query: 126 MPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFY 185
+ VVQI+G ++AP K+ +WP SSLFQWLNFKW+ + TIQGSGT++G+G+NWW L T+
Sbjct: 125 KEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTYQ 184
Query: 186 TNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPEN 245
T + K+IP MKPTALRFYSS NVTVRDI+I+NSP CHLKFD+S VKV+NITISSPEN
Sbjct: 185 TQTRN-KYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPEN 244
Query: 246 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 305
SPNTDGIHLQNT++VEIQHSNI CGDDCVSIQTG SN+HIHHINCGPGHGIS+GGLGKDK
Sbjct: 245 SPNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDK 304
Query: 306 SAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK 365
S ACVS+I+V++ISIQNTL+GVRIKTWQGG+G VKN++FSNIQV DVKVPI+IDQYYCDK
Sbjct: 305 SVACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDK 364
Query: 366 YRCKNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQS 425
+CKNQT AV+ISG+ Y+ I+GS+TVQP+ +ACS+ +PC+DVDL+DI+L+PS RG Q+
Sbjct: 365 SKCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQT 424
Query: 426 -----SLCLNSYGKSQAPLLPTSIDYCLRR---GGGAVRRIARSHDHVC 467
+LC NSYGK+Q PL+P+SI YCLR+ GG ++++RS+D +C
Sbjct: 425 HQQQQALCWNSYGKTQGPLVPSSIGYCLRKSNIGGYYSQKVSRSYDKIC 468
BLAST of Lsi04G005670 vs. TAIR 10
Match:
AT1G10640.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 434.1 bits (1115), Expect = 1.4e-121
Identity = 213/432 (49.31%), Postives = 286/432 (66.20%), Query Frame = 0
Query: 58 PAPLPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 117
P PLP Q S VFN++ FGAKG+G DD+KA AAW AAC+I + + +P +Y +L+
Sbjct: 107 PPPLPP--QKGSQVFNVMDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVG 166
Query: 118 PITLNGP-CMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGF 177
PI+ +GP C +V Q+DG ++AP TW K L W++F L +QG G +DG+G
Sbjct: 167 PISFSGPYCQANIVFQLDGTIIAPTDSKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGRGS 226
Query: 178 NWWTL-STFYTNKKKL---------------------KHIPDMKPTALRFYSSYNVTVRD 237
WW S F + KL + +P +KPTALRF S+ V V
Sbjct: 227 GWWQQDSPFIDSDTKLIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTG 286
Query: 238 ITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCV 297
ITI NSPQCHLKFD+ V V +I +SSP +SPNTDGIHLQNT+DV I + + CGDDC+
Sbjct: 287 ITIQNSPQCHLKFDDCVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCI 346
Query: 298 SIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQG 357
SIQTGCSN+ +H++NCGPGHGIS+G LGK+ + ACVSNI V+++++ NT++GVRIKTWQG
Sbjct: 347 SIQTGCSNVFVHNVNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQG 406
Query: 358 GIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGITYDQIIGSYTVQPI 417
G+GSVK + FSNIQ++ V++PI I+Q+YCD +CKNQT AVA+ G+TY++I G+YTV+P+
Sbjct: 407 GVGSVKGIIFSNIQLNQVQIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPV 466
Query: 418 HLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQAPLLPTSIDYCLRRGGGA 467
H ACS PCVDV L I+LKP E + C ++G+ P LP ID CL+ G
Sbjct: 467 HFACSDNFPCVDVQLSSIELKPVQEKYRMYDAYCWQTFGELNTPTLP-PID-CLKIGKPP 526
BLAST of Lsi04G005670 vs. TAIR 10
Match:
AT1G60590.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 424.9 bits (1091), Expect = 8.6e-119
Identity = 208/432 (48.15%), Postives = 285/432 (65.97%), Query Frame = 0
Query: 58 PAPLPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 117
P PLP S FN+L FGAKG+G+SDD++A AAW +AC++ +T+ IP Y FL+
Sbjct: 109 PTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVG 168
Query: 118 PITLNGP-CMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGF 177
PI+ +GP C +V Q++G ++AP +W L W+ F L TIQG+G +DG+G
Sbjct: 169 PISFSGPYCQANIVFQLEGMIVAPTDTESW-GGGLMWWIEFTKLSGITIQGNGVIDGRGT 228
Query: 178 NWWT---LSTFYTN---------------------KKKLK-HIPDMKPTALRFYSSYNVT 237
WW LS + + + +L +P +KPTALRFY S +VT
Sbjct: 229 VWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVT 288
Query: 238 VRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGD 297
V ITI NSPQCHLKFDN V V ++ +SSP +SPNTDGIHLQNT+DV I + + CGD
Sbjct: 289 VTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGD 348
Query: 298 DCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKT 357
DC+SIQTGCSN+++H++NCGPGHGIS+G LGKD + ACVSNI V+++ + NT++GVRIKT
Sbjct: 349 DCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKT 408
Query: 358 WQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGITYDQIIGSYTV 417
WQGGIGSVK + FSNIQ+++V++PI+IDQ+YCD +C N T AV++ G+TY++I G+YTV
Sbjct: 409 WQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTV 468
Query: 418 QPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQAPLLPTSIDYCLRRG 464
+P+H ACS PC+DV L I+LKP C ++G+ + +P ID CL+ G
Sbjct: 469 KPVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVP-PID-CLQIG 528
BLAST of Lsi04G005670 vs. TAIR 10
Match:
AT5G14650.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 393.7 bits (1010), Expect = 2.1e-109
Identity = 205/433 (47.34%), Postives = 270/433 (62.36%), Query Frame = 0
Query: 10 LSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQS 69
LSL L++SL SS +AR + K + +G +G
Sbjct: 9 LSLNFLLLISLF-SSRFGTCDARYSVYWKGNRRSIAEGGSSG------------------ 68
Query: 70 TVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNG-PCMPQ 129
N+L GAKG+G SDD+KA AW+ AC++ +TL +PS FL+ P++ G C +
Sbjct: 69 -TINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKEK 128
Query: 130 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNK 189
+V Q++G ++AP S W S L QW+ FK L TI+G G +DG+G WW N
Sbjct: 129 IVFQLEGKIIAPTSASAW-GSGLLQWIEFKALQGITIKGKGIIDGRGSVWW-------ND 188
Query: 190 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 249
+P KPTALRFY S VTV ITI NSPQ HLKFDN +++V + T SSP +SPN
Sbjct: 189 MMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPN 248
Query: 250 TDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAA 309
TDGIHLQN+QD I S + CGDDC+SIQTGCSNI+IH ++CGPGHGIS+GGLGKD + A
Sbjct: 249 TDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKA 308
Query: 310 CVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRC 369
CVSNI V+++++ T +GVRIK+WQGG GSVK V FSNIQVS+V PI+IDQYYCD C
Sbjct: 309 CVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGC 368
Query: 370 KNQTGAVAISGITYDQIIGSYTVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQSSLC 429
N+T AVA+S I Y I G+YT +P+ ACS +PC + L I+LKP++ C
Sbjct: 369 HNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPFC 413
Query: 430 LNSYGKSQAPLLP 442
++G+ + LP
Sbjct: 429 WKAHGELKTKTLP 413
BLAST of Lsi04G005670 vs. TAIR 10
Match:
AT1G56710.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 346.7 bits (888), Expect = 3.0e-95
Identity = 180/404 (44.55%), Postives = 248/404 (61.39%), Query Frame = 0
Query: 57 APAPLPDYNQTQS---TVFNILSFGAKGNGISDDSKALLAAWKAACEIPG---ATLEIPS 116
+PAP P YN + TVF++ SFGA G+ +DD+ A AW AAC G A L +P
Sbjct: 30 SPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVPY 89
Query: 117 QYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGT 176
+ FL+KP T NGPC LV+QIDG +++P +WP + QW+ F ++ +IQGSG
Sbjct: 90 TFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSGV 149
Query: 177 VDGQGFNWWTLSTFYTNKKKLKHI----PDMKPTALRFYSSYNVTVRDITIINSPQCHLK 236
++G+G WW L K L P P A+R + S V ++ I +NS Q H++
Sbjct: 150 INGRGQKWWNLPC--KPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVR 209
Query: 237 FDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIH 296
FDN V VD++ I +P +SPNTDGIH++NT +V+I++S I GDDC+SI GC N+ I
Sbjct: 210 FDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIK 269
Query: 297 HINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSN 356
++ CGP HGIS+G LG S A VSNI V N +I N+ +GVRIKTWQGG GSV + FSN
Sbjct: 270 NVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSN 329
Query: 357 IQVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGITYDQIIGSYTVQ--PIHLACSSQIPC 416
I + +V+ PIMIDQYYC C NQT AV IS + Y I G+Y ++ PIH CS +PC
Sbjct: 330 ILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPC 389
Query: 417 VDVDLIDIQLKPSSESRGFQSSLCLNSYGKSQAPLLPTSIDYCL 449
++ L ++ L P S+ + ++ C N+YG + + P YCL
Sbjct: 390 TNLTLTEVDLFP-SKGQHLENPFCWNAYGSMKITVPPV---YCL 427
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q949Z1 | 4.5e-88 | 40.39 | Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... | [more] |
| Q94AJ5 | 8.5e-79 | 40.11 | Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... | [more] |
| P43212 | 2.7e-77 | 38.64 | Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1 | [more] |
| Q7M1E7 | 6.7e-76 | 39.10 | Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1 | [more] |
| Q8RY29 | 8.2e-74 | 41.60 | Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3BEF8 | 8.1e-250 | 93.79 | polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1 | [more] |
| A0A5D3CVS9 | 4.2e-246 | 93.89 | Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... | [more] |
| A0A6J1HSE8 | 2.4e-241 | 87.58 | polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=... | [more] |
| A0A6J1FZF6 | 1.2e-240 | 87.58 | polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11144... | [more] |
| A0A6J1FZB5 | 2.9e-239 | 87.39 | polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11144... | [more] |
