Lsi04G005480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G005480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Carbonic anhydrase
Location	chr04: 5029328 .. 5032808 (-)
RNA-Seq Expression	Lsi04G005480
Synteny	Lsi04G005480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CACTTCAGTTCATTATTTCGGGTCCAAACAGCTGCTTTAACCACCTCACATTCCCCTCTATTTCCAATCACAAATCACAGCCGTTCATCCAAATTCTTCTCCAAATCCACGGCTGTGATCAAATCAAGCTTTTCATTCACACCATTGAAAATTTATAGCACAATCACATTCACACCATCAAAATCACACATCTTTCCCTTTTGCAATTTGGATAATTCCAAAATTTCCAATTTGTATTATACTCTCTTCTTTTTTTTTTTAATTTCAAAATTTGTCAACTAATACGTATAATTACATGCCGATTATAGTATATTAGAATTACGCGTAAATGAATAATTAAATTTTGCAAGAAGCCAAAAGAAGGAGACTTTTTCCAACGTCATTTTCCCATTATCTAAAAATTATGTCCATTCTCTAAATAAGAAAATGCCAATTGGGTAATTAATTTCTTTAATTTCATCAATATCTGGTTGCTTCCATTATATATATGGGACTATCTTCAGCAACATGGTATAGCTTTTGTTTGCTGATTAAGTTTTCCTTTCCAACAACATTAATAATCTGATAAGGCCGCAGATTTTTACACACAATTTTAGAGGTTTGTTCATCAAATCGCTTCATTTTCTTCTTCTTCTTCTTCGGATCCTCATCATAATTCTCTTGATTTTCAGAAGAAAAATTATTGGGATTTCTTTTTATATCTATATTACATGATTCTTTATCAAAATTTTGTAGAAAAAATGATGATCTGAATCCTCCACCGACACCGTTTTTATTAGTGTTATTGCTCCGGCGATCTCTTAAGTTTTGTTTTTGAAATTCTTTTGGGGACTGATTTGGAATTATCAGGAAGACATGGCGCAAGAGTCGTACGAGGAAGCAATTGCCGGACTCGCAAAGCTTCTCAGGTACTGGATCCATTTGCTTCAATCCTTTCTGTTCTGTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTCCGTTTAATTCCCTAAAGCCGGCCACCGCTAATTAGGGCGGCGGCCCACCACGTAGGACCGACACGAATTGATTGTCCAAAAGAACGTTCCGTAACGGTTTCCGTTTTAAATGTCATTATGTAGTGAGAAAGCTGACCTTCAGGATGCTGCCGCCGCAAAGATCCGGCAAATAACCGCCGAATTGGCCGGGACCACTGCCGGTTCAAATGGCTTCGACCCGGTTGATAGAATCAGAACCGGGTTTACCCATTTCAAGAAATCCAAATTCGAGTACGTTTCTTTTTCTCCATCCGTCCTTTGAAATTTACTTCTTTTGATTTGTTTTTTTCGAATATGCTGTTTGGTTGATTCCCTCTTAATTATCCTTTAGATGATGAATCTGCCTGTCCTTATCCCATCACGCTTGTTCACAAATTCATTTCAAATCATAATTAATTATAATTAATTTTTTTATTGGGGTTCCCCAGGACGAATCCTGATTTGTATGGTCAACTTGCAAAAAGCCAAAGCCCAAAGGTAAGGAATTCACAATTTCAGAAAAATACCGTGGGGAATAAATGTAAATTATAATTTCTTCGAGGGCTATGATAAGGGCACCACCGCAATTATTGTGGCTAGATAAGGTGTTGAAATCAAAAAGCCAAAAAGGAGAAACCAGCCAATAACAAATGTCCACGTGTAAATGGCAGTTTTTGGTATTTGCGTGTTCGGATTCCCGAGTTTGCCCTTCGCACATATTGAATTTTCAACCAGGGGAGGCCTTTGTGGTTCGTAACATCGCCAGCATGGTCCCACCATTTGACAAGGTTGGCCTGTCGCTATCAATTTATTTCATTAATTAAATATAATTAATTTCAACTTATTAACTTATTATTTATTTATTTTAAATTTGGCTGTTTGTAGAAAAAATATTCAGGAGCGGGGGCTGCAATTGAATATGCAGTATTGCATCTTAAGGTGGAAAATTTTATATATTATTTAATAGTTTAATGATCACCCCCATTTTCTTGGGATTGTATTGTCATTATTATTTTTTATTCTAATATAAAAAAAAATCGAAATTTAATTAATGTAGGTGGAGAATATAGTGGTGATTGGACATAGCTGCTGTGGGGGCATTAAGGGCCTCATGTCCATCCCAGATGATGGAACCATTTCCAGGCAAGTTCCTCTTTTTTCTTTTCTTTTTTTTTTTTTTTTAATAAGTTTGATTTTGAAACAACATTATTCCTAAATTTTTAAAAATCTAATAAGTTTTTAAATTTTCAATTTACTGTCTAAAATTTTATTTATGTGAAAGATTTGTTAGACACAAAAATAAAAGTTGGTAGACTTATTAAACATAAATGTAAAAGTTCATAAGCTTATTTAATACATTCTTAAGTTGAGAGACAAAGTAGATAAGATTTTTATAATTTAATCAATAATGGATAAATTTAATAAATTTAAAGTTTTGATTAAGAGTTAATTTATTTTGACCCACTTGTAATACACATGTAAATAAAAGTTAGGTGGCAATCGGCGTAGGTGAGTTCTTCCCATGTGATGAAATGGGTAAAAAAAAAAATAGAGTGAGTTAGGGTTAGTTTAGGGTTGGCTTTTAATGTAGTGGTGAATCACAGAGGAATACTCTTTCTCAATAAAAGCCCTTTAACCTTTCCACTCTGGTGGTACCATATTCTTGGAGGAACATCTATATATATATATATATATATATATATATATATATAAAGCTTATAAATTGCAACTGATCAACTTTTGCATTTTACTATTTAGTGCGGAATGATAAGTCCAAGTCCTTTACTTCCCACCTTTTGTCTTTACTTTACAGATCAATCCTTTTCTTTTTGGACCTAAAAGAAGTCATAATAATTTTATTACTATTTTCTTTGGCAATTTTGTGGGAATTTTTCCATTAAAAAACCCACTTTTTAGTTTTCACCTATCTCTCAGCCTGGATAAGAGAAGTTACAGCTCATTCCAAATCCAGATCCTATTTTCTCTCTGTTTGATTCCAAGTGCAAGATATGTCTTGGGATTTACCTTTTTAAGCAAAAGTAACACAGTTTCTTTAATTTGCAGTGATTTCATTGAGAATTGGGTCCAAATTTGCACACCGGCCAAAACCAAGACCCAATCAGATTGCACCGATTTGAGTTTCGAGGACAAATGCACAAACTGCGAGAAGGTATATTAATAATTTCTAAAAATTCATTCTAGATTCACATACAAAATCGAAATTTTTTTTAGTTTATTTATCGTGGGTCATATTAACTTGTTTTTAAAAATAGAGTGAGACAGAATTTGATTCTTTGTAATACCAAACATTAATATACATTGCAGGAGGCTGTGAATGTTTCCCTTGGAAACTTGCTGTCATATCCTTTTGTAAGAGAAGCTGTCGTCAACAAGAAACTGTTCATAAGGGGTGCACATTACAACTTTGTTTCTGGAGCTTTTGAGCTTTGGAATCTTGACTTTAACATTTCAACTTCTCTAGCTGTT

mRNA sequence

CACTTCAGTTCATTATTTCGGGTCCAAACAGCTGCTTTAACCACCTCACATTCCCCTCTATTTCCAATCACAAATCACAGCCGTTCATCCAAATTCTTCTCCAAATCCACGGCTGTGATCAAATCAAGCTTTTCATTCACACCATTGAAAATTTATAGCACAATCACATTCACACCATCAAAATCACACATCTTTCCCTTTTGCAATTTGGATAATTCCAAAATTTCCAATTTAAAAAATGATGATCTGAATCCTCCACCGACACCGTTTTTATTAGAAGACATGGCGCAAGAGTCGTACGAGGAAGCAATTGCCGGACTCGCAAAGCTTCTCAGCCGGCCACCGCTAATTAGGGCGGCGGCCCACCACGTAGGACCGACACGAATTGATTGTCCAAAAGAACGTTCCGTAACGGTTTCCGTTTTAAATGTCATTATTGAGAAAGCTGACCTTCAGGATGCTGCCGCCGCAAAGATCCGGCAAATAACCGCCGAATTGGCCGGGACCACTGCCGGTTCAAATGGCTTCGACCCGGTTGATAGAATCAGAACCGGGTTTACCCATTTCAAGAAATCCAAATTCGAGACGAATCCTGATTTGTATGGTCAACTTGCAAAAAGCCAAAGCCCAAAGTTTTTGGTATTTGCGTGTTCGGATTCCCGAGTTTGCCCTTCGCACATATTGAATTTTCAACCAGGGGAGGCCTTTGTGGTTCGTAACATCGCCAGCATGGTCCCACCATTTGACAAGAAAAAATATTCAGGAGCGGGGGCTGCAATTGAATATGCAGTATTGCATCTTAAGGTGGAGAATATAGTGGTGATTGGACATAGCTGCTGTGGGGGCATTAAGGGCCTCATGTCCATCCCAGATGATGGAACCATTTCCAGGCAAGTGGCAATCGGCGTAGAATTGGGTCCAAATTTGCACACCGGCCAAAACCAAGACCCAATCAGATTGCACCGATTTGAGTTTCGAGGACAAATGCACAAACTGCGAGAAGCTGTCGTCAACAAGAAACTGTTCATAAGGGGTGCACATTACAACTTTGTTTCTGGAGCTTTTGAGCTTTGGAATCTTGACTTTAACATTTCAACTTCTCTAGCTGTT

Coding sequence (CDS)

CACTTCAGTTCATTATTTCGGGTCCAAACAGCTGCTTTAACCACCTCACATTCCCCTCTATTTCCAATCACAAATCACAGCCGTTCATCCAAATTCTTCTCCAAATCCACGGCTGTGATCAAATCAAGCTTTTCATTCACACCATTGAAAATTTATAGCACAATCACATTCACACCATCAAAATCACACATCTTTCCCTTTTGCAATTTGGATAATTCCAAAATTTCCAATTTAAAAAATGATGATCTGAATCCTCCACCGACACCGTTTTTATTAGAAGACATGGCGCAAGAGTCGTACGAGGAAGCAATTGCCGGACTCGCAAAGCTTCTCAGCCGGCCACCGCTAATTAGGGCGGCGGCCCACCACGTAGGACCGACACGAATTGATTGTCCAAAAGAACGTTCCGTAACGGTTTCCGTTTTAAATGTCATTATTGAGAAAGCTGACCTTCAGGATGCTGCCGCCGCAAAGATCCGGCAAATAACCGCCGAATTGGCCGGGACCACTGCCGGTTCAAATGGCTTCGACCCGGTTGATAGAATCAGAACCGGGTTTACCCATTTCAAGAAATCCAAATTCGAGACGAATCCTGATTTGTATGGTCAACTTGCAAAAAGCCAAAGCCCAAAGTTTTTGGTATTTGCGTGTTCGGATTCCCGAGTTTGCCCTTCGCACATATTGAATTTTCAACCAGGGGAGGCCTTTGTGGTTCGTAACATCGCCAGCATGGTCCCACCATTTGACAAGAAAAAATATTCAGGAGCGGGGGCTGCAATTGAATATGCAGTATTGCATCTTAAGGTGGAGAATATAGTGGTGATTGGACATAGCTGCTGTGGGGGCATTAAGGGCCTCATGTCCATCCCAGATGATGGAACCATTTCCAGGCAAGTGGCAATCGGCGTAGAATTGGGTCCAAATTTGCACACCGGCCAAAACCAAGACCCAATCAGATTGCACCGATTTGAGTTTCGAGGACAAATGCACAAACTGCGAGAAGCTGTCGTCAACAAGAAACTGTTCATAAGGGGTGCACATTACAACTTTGTTTCTGGAGCTTTTGAGCTTTGGAATCTTGACTTTAACATTTCAACTTCTCTAGCTGTT

Protein sequence

HFSSLFRVQTAALTTSHSPLFPITNHSRSSKFFSKSTAVIKSSFSFTPLKIYSTITFTPSKSHIFPFCNLDNSKISNLKNDDLNPPPTPFLLEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDAAAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVVIGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHTGQNQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV

Homology

[Close]

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot
Match: P27141 (Carbonic anhydrase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 8.0e-72
Identity = 165/358 (46.09%), Postives = 210/358 (58.66%), Query Frame = 0


Query: 51  IYSTITFTPSKSHI------FPFCNLDNSKISN-----LKNDDLNPPPT----PFLLEDM 110
           I S +T +P+++ +        F  L NS  S      ++N+ +   PT    P L E+M
Sbjct: 6   INSCLTISPAQASLKKPTRPVAFARLSNSSSSTSVPSLIRNEPVFAAPTPIINPILREEM 65

Query: 111 AQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDAA 170
           A+ESYE+AIA L KLLS                                  EK +L   A
Sbjct: 66  AKESYEQAIAALEKLLS----------------------------------EKGELGPIA 125

Query: 171 AAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLVF 230
           AA++ QITAEL  ++ GS  FDPV+ ++ GF HFK  K+E NP LYG+L+K QSPKF+VF
Sbjct: 126 AARVDQITAELQ-SSDGSKPFDPVEHMKAGFIHFKTEKYEKNPALYGELSKGQSPKFMVF 185

Query: 231 ACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVVI 290
           ACSDSRVCPSH+LNFQPGEAFVVRNIA+MVP +DK +YSG GAAIEYAVLHLKVENIVVI
Sbjct: 186 ACSDSRVCPSHVLNFQPGEAFVVRNIANMVPAYDKTRYSGVGAAIEYAVLHLKVENIVVI 245

Query: 291 GHSCCGGIKGLMSIPDDGT-----ISRQVAIGVELGPNLHTGQNQDPI------------ 350
           GHS CGGIKGLMS+P DG+     I   V IG+     +  G++ D              
Sbjct: 246 GHSACGGIKGLMSLPADGSESTAFIEDWVKIGLPAKAKVQ-GEHVDKCFADQCTACEKEA 305

Query: 351 ------RLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                  L  + F      +RE +V K L ++G HY+FV+G FELW L+F +S SL+V
Sbjct: 306 VNVSLGNLLTYPF------VREGLVKKTLALKGGHYDFVNGGFELWGLEFGLSPSLSV 321

[Close]

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot
Match: P27140 (Beta carbonic anhydrase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA1 PE=1 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-68
Identity = 162/370 (43.78%), Postives = 206/370 (55.68%), Query Frame = 0


Query: 30  SKFFSKSTAVIKSSFSFTPLKIYSTITFTP--SKSHIFPFCNLDNSKISNLKNDDLNPPP 89
           S FF  S +  +SS     L+  ST+   P  S S      +   S  + ++N+ +   P
Sbjct: 7   SGFFLTSLSPSQSSLQKLSLRTSSTVACLPPASSSSSSSSSSSSRSVPTLIRNEPVFAAP 66

Query: 90  TPFLL----EDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLN 149
            P +     E+M  E+Y+EAI  L KLL                                
Sbjct: 67  APIIAPYWSEEMGTEAYDEAIEALKKLL-------------------------------- 126

Query: 150 VIIEKADLQDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYG 209
             IEK +L+  AAAK+ QITA L  GT++    FDPV+ I+ GF  FKK K+ETNP LYG
Sbjct: 127 --IEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFIKFKKEKYETNPALYG 186

Query: 210 QLAKSQSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEY 269
           +LAK QSPK++VFACSDSRVCPSH+L+FQPG+AFVVRNIA+MVPPFDK KY G GAAIEY
Sbjct: 187 ELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPPFDKVKYGGVGAAIEY 246

Query: 270 AVLHLKVENIVVIGHSCCGGIKGLMSIPDDGT-------------ISRQVAIGVELGPNL 329
           AVLHLKVENIVVIGHS CGGIKGLMS P DG              +  +  +  ELG + 
Sbjct: 247 AVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVISELGDSA 306

Query: 330 HTGQ---------NQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNL 371
              Q         N     L  + F      +RE +V   L ++G +Y+FV GAFELW L
Sbjct: 307 FEDQCGRCEREAVNVSLANLLTYPF------VREGLVKGTLALKGGYYDFVKGAFELWGL 336

[Close]

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot
Match: P42737 (Beta carbonic anhydrase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA2 PE=1 SV=3)

HSP 1 Score: 258.1 bits (658), Expect = 1.6e-67
Identity = 148/291 (50.86%), Postives = 176/291 (60.48%), Query Frame = 0


Query: 92  LEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADL 151
           L  M  ESYE+AI  L KLL                                  IEK DL
Sbjct: 70  LRRMGNESYEDAIEALKKLL----------------------------------IEKDDL 129

Query: 152 QDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSP 211
           +D AAAK+++ITAEL A +++ S  FDPV+RI+ GF  FKK K+ETNP LYG+LAK QSP
Sbjct: 130 KDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFVTFKKEKYETNPALYGELAKGQSP 189

Query: 212 KFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVE 271
           K++VFACSDSRVCPSH+L+F PG+AFVVRNIA+MVPPFDK KY+G GAAIEYAVLHLKVE
Sbjct: 190 KYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKVE 249

Query: 272 NIVVIGHSCCGGIKGLMSIPDDGTISRQV----------AIGVELGPNLHTGQNQDPIRL 331
           NIVVIGHS CGGIKGLMS P DG  S             A    L  +  +       R 
Sbjct: 250 NIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVLAESESSAFEDQCGRC 309

Query: 332 HRFEFRGQMHKL------REAVVNKKLFIRGAHYNFVSGAFELWNLDFNIS 366
            R      +  L      RE VV   L ++G +Y+FV+G+FELW L F IS
Sbjct: 310 EREAVNVSLANLLTYPFVREGVVKGTLALKGGYYDFVNGSFELWELQFGIS 326

[Close]

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot
Match: P17067 (Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.2e-65
Identity = 156/361 (43.21%), Postives = 204/361 (56.51%), Query Frame = 0


Query: 32  FFSKSTAVIKSSFSFTPLKIY---STITFTPSKSHIFPFCNLDNSKISNLKNDDLNPPPT 91
           F   S +  K+S   T L+ +   S  T + S S  FP    D    ++      +P  T
Sbjct: 9   FSLSSLSPAKTSTKRTTLRPFVSASLNTSSSSSSSTFPSLIQDKPVFAS-----SSPIIT 68

Query: 92  PFLLEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEK 151
           P L E+M  + Y+EAI  L KLL                                   EK
Sbjct: 69  PVLREEMG-KGYDEAIEELQKLLR----------------------------------EK 128

Query: 152 ADLQDAAAAKIRQITAELAGTTAGSNGF---DPVDRIRTGFTHFKKSKFETNPDLYGQLA 211
            +L+  AA K+ QITA+L GTT+ S+G    +  +RI+TGF HFKK K++ NP LYG+LA
Sbjct: 129 TELKATAAEKVEQITAQL-GTTSSSDGIPKSEASERIKTGFLHFKKEKYDKNPALYGELA 188

Query: 212 KSQSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVL 271
           K QSP F+VFACSDSRVCPSH+L+FQPGEAFVVRN+A++VPP+D+ KY+G GAAIEYAVL
Sbjct: 189 KGQSPPFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQAKYAGTGAAIEYAVL 248

Query: 272 HLKVENIVVIGHSCCGGIKGLMSIPDDGT-----ISRQVAIGVELGPNLHTGQNQDPI-- 331
           HLKV NIVVIGHS CGGIKGL+S P DGT     I   V IG+     +       P   
Sbjct: 249 HLKVSNIVVIGHSACGGIKGLLSFPFDGTYSTDFIEEWVKIGLPAKAKVKAQHGDAPFAE 308

Query: 332 ---RLHRFEFRGQMHKL------REAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLA 371
                 +      +  L      RE +VNK L ++G +Y+FV G+FELW L+F +S++ +
Sbjct: 309 LCTHCEKEAVNASLGNLLTYPFVREGLVNKTLALKGGYYDFVKGSFELWGLEFGLSSTFS 328

[Close]

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot
Match: Q94CE4 (Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-64
Identity = 143/294 (48.64%), Postives = 175/294 (59.52%), Query Frame = 0


Query: 93  EDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQ 152
           ++MA ESYE AI GL  LLS                                   KADL 
Sbjct: 21  DEMATESYEAAIKGLNDLLS----------------------------------TKADLG 80

Query: 153 DAAAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKF 212
           + AAAKI+ +TAEL      S+  D ++RI+TGFT FK  K+  N  L+  LAK+Q+PKF
Sbjct: 81  NVAAAKIKALTAEL--KELDSSNSDAIERIKTGFTQFKTEKYLKNSTLFNHLAKTQTPKF 140

Query: 213 LVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENI 272
           LVFACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFD+K++SG GAA+EYAV+HLKVENI
Sbjct: 141 LVFACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDQKRHSGVGAAVEYAVVHLKVENI 200

Query: 273 VVIGHSCCGGIKGLMSIPDDG--TISRQVAIGVELGPNLHTGQNQDPIRLHRFEFRGQMH 332
           +VIGHSCCGGIKGLMSI DD   T S  +   V++G +      ++   L   +   +  
Sbjct: 201 LVIGHSCCGGIKGLMSIEDDAAPTQSDFIENWVKIGASARNKIKEEHKDLSYDDQCNKCE 260

Query: 333 K---------------LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLA 370
           K               +R  VV   L IRG HYNFV G F+LW LDF  + + A
Sbjct: 261 KEAVNVSLGNLLSYPFVRAEVVKNTLAIRGGHYNFVKGTFDLWELDFKTTPAFA 278

[Close]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL
Match: A0A5A7SWR5 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003160 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 3.3e-97
Identity = 202/292 (69.18%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHTGQNQDPIRLHRFEFR-------- 334
           IGHSCCGGIKGLMSIPDDG  SRQ  + +       T  N + +    FE +        
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSRQNWVQICTPAKNKTQSNCNDL---SFEDKCTECEKEA 240

Query: 335 -----GQMHK---LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                G +     +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 VNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 255

[Close]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL
Match: A0A1S3BEI1 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103489011 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 8.2e-96
Identity = 199/295 (67.46%), Postives = 211/295 (71.53%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHT-GQNQDPIRLHRFEFRGQMHK-- 334
           IGHSCCGGIKGLMSIPDDG  S      +E    + T  +N+     +   F  +  +  
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSSDF---IENWVQICTPAKNKTQSNCNDLSFEDKCTECE 240

Query: 335 ----------------LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                           +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 KEAVNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 258

[Close]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL
Match: A0A5D3CXD3 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001010 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 8.2e-96
Identity = 199/295 (67.46%), Postives = 211/295 (71.53%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHT-GQNQDPIRLHRFEFRGQMHK-- 334
           IGHSCCGGIKGLMSIPDDG  S      +E    + T  +N+     +   F  +  +  
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSSDF---IENWVQICTPAKNKTQSNCNDLSFEDKCTECE 240

Query: 335 ----------------LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                           +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 KEAVNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 258

[Close]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL
Match: E5GBJ5 (Carbonic anhydrase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 8.2e-96
Identity = 199/295 (67.46%), Postives = 211/295 (71.53%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHT-GQNQDPIRLHRFEFRGQMHK-- 334
           IGHSCCGGIKGLMSIPDDG  S      +E    + T  +N+     +   F  +  +  
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSSDF---IENWVQICTPAKNKTQSNCNDLSFEDKCTECE 240

Query: 335 ----------------LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                           +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 KEAVNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 258

[Close]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL
Match: A0A6J1DBH1 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111019165 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 5.3e-95
Identity = 196/300 (65.33%), Postives = 213/300 (71.00%), Query Frame = 0


Query: 88  TPFLLEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIE 147
           TP + EDMAQESYEEAIAGL KLLS                                  E
Sbjct: 14  TPKVSEDMAQESYEEAIAGLKKLLS----------------------------------E 73

Query: 148 KADLQDAAAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKS 207
           KADLQDAAAAKIRQITAEL+ TTAGS+ FDPVDRI+TGFTHFKKSKFETNPDL+GQLAK 
Sbjct: 74  KADLQDAAAAKIRQITAELSATTAGSDSFDPVDRIKTGFTHFKKSKFETNPDLFGQLAKG 133

Query: 208 QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHL 267
           QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIA+MVPP+DK KYSG GAA+EYA+LHL
Sbjct: 134 QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIANMVPPYDKTKYSGVGAAVEYAILHL 193

Query: 268 KVENIVVIGHSCCGGIKGLMSIPDDGTISRQV----------------AIGVELG-PNLH 327
           KVENIVVIGHSCCGGIKGLMSIPDDGTIS                   + G EL   +  
Sbjct: 194 KVENIVVIGHSCCGGIKGLMSIPDDGTISSDFIENWVKICAPAKTKIQSEGTELSFEDKC 253

Query: 328 TGQNQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
           T   ++ + +           +RE VVNKKLFIRGAHYNFVSGAFELWNLDF I+ SLAV
Sbjct: 254 TNCEKEAVNVSLGNLLSYPF-VRECVVNKKLFIRGAHYNFVSGAFELWNLDFKITPSLAV 278

[Close]

BLAST of Lsi04G005480 vs. NCBI nr
Match: XP_038893413.1 (carbonic anhydrase 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 370.9 bits (951), Expect = 1.3e-98
Identity = 208/295 (70.51%), Postives = 216/295 (73.22%), Query Frame = 0


Query: 93  EDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQ 152
           EDMAQESYEEAIAGL KLLS                                  EKADLQ
Sbjct: 18  EDMAQESYEEAIAGLTKLLS----------------------------------EKADLQ 77

Query: 153 DAAAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKF 212
           DAAAAKIRQIT ELAGTTA SNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAK QSPKF
Sbjct: 78  DAAAAKIRQITTELAGTTANSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKGQSPKF 137

Query: 213 LVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENI 272
           LVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENI
Sbjct: 138 LVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENI 197

Query: 273 VVIGHSCCGGIKGLMSIPDDGTISRQ-VAIGVELGPNLHTGQNQDPIRLHRFEFR----- 332
           VVIGHSCCGGIKGLMSIPDDGTIS   +   V++     T + Q       FE +     
Sbjct: 198 VVIGHSCCGGIKGLMSIPDDGTISSDFIENWVQICTPART-KTQSYCTDLSFEDKCTNCE 257

Query: 333 --------GQMHK---LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                   G +     +REAVVNK+LFIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 258 KEAVNVSLGNLLSYPFVREAVVNKQLFIRGAHYNFVSGAFELWNLDFNISPSLAV 277

[Close]

BLAST of Lsi04G005480 vs. NCBI nr
Match: XP_038893414.1 (carbonic anhydrase 2-like isoform X2 [Benincasa hispida] >XP_038893415.1 carbonic anhydrase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 366.7 bits (940), Expect = 2.4e-97
Identity = 206/293 (70.31%), Postives = 214/293 (73.04%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLTKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQIT ELAGTTA SNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITTELAGTTANSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQ-VAIGVELGPNLHTGQNQDPIRLHRFEFR------- 334
           IGHSCCGGIKGLMSIPDDGTIS   +   V++     T + Q       FE +       
Sbjct: 181 IGHSCCGGIKGLMSIPDDGTISSDFIENWVQICTPART-KTQSYCTDLSFEDKCTNCEKE 240

Query: 335 ------GQMHK---LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                 G +     +REAVVNK+LFIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 AVNVSLGNLLSYPFVREAVVNKQLFIRGAHYNFVSGAFELWNLDFNISPSLAV 258

[Close]

BLAST of Lsi04G005480 vs. NCBI nr
Match: KAA0034316.1 (carbonic anhydrase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 6.9e-97
Identity = 202/292 (69.18%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHTGQNQDPIRLHRFEFR-------- 334
           IGHSCCGGIKGLMSIPDDG  SRQ  + +       T  N + +    FE +        
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSRQNWVQICTPAKNKTQSNCNDL---SFEDKCTECEKEA 240

Query: 335 -----GQMHK---LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                G +     +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 VNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 255

[Close]

BLAST of Lsi04G005480 vs. NCBI nr
Match: XP_008446208.1 (PREDICTED: carbonic anhydrase 2-like [Cucumis melo] >XP_008446210.1 PREDICTED: carbonic anhydrase 2-like [Cucumis melo] >ADN33836.1 carbonic anhydrase [Cucumis melo subsp. melo] >TYK15604.1 carbonic anhydrase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 360.5 bits (924), Expect = 1.7e-95
Identity = 199/295 (67.46%), Postives = 211/295 (71.53%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           MAQESYEEAIAGL+KLLS                                  EKADLQDA
Sbjct: 1   MAQESYEEAIAGLSKLLS----------------------------------EKADLQDA 60

Query: 155 AAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFLV 214
           AAAKIRQITAEL GTTA SNGFDPVDRI+TGFTHFKKSKFETNPDLYGQLAK QSPKFLV
Sbjct: 61  AAAKIRQITAELGGTTACSNGFDPVDRIKTGFTHFKKSKFETNPDLYGQLAKGQSPKFLV 120

Query: 215 FACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIVV 274
           FACSDSRVCPSHILNFQPGEAFVVRNIA+MVPPFDK KYSGAGAAIEYAVLHLKVENIVV
Sbjct: 121 FACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDKTKYSGAGAAIEYAVLHLKVENIVV 180

Query: 275 IGHSCCGGIKGLMSIPDDGTISRQVAIGVELGPNLHT-GQNQDPIRLHRFEFRGQMHK-- 334
           IGHSCCGGIKGLMSIPDDG  S      +E    + T  +N+     +   F  +  +  
Sbjct: 181 IGHSCCGGIKGLMSIPDDGAFSSDF---IENWVQICTPAKNKTQSNCNDLSFEDKCTECE 240

Query: 335 ----------------LREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
                           +REAVVNKK+FIRGAHYNFVSGAFELWNLDFNIS SLAV
Sbjct: 241 KEAVNVSLGNLLSYPFVREAVVNKKVFIRGAHYNFVSGAFELWNLDFNISPSLAV 258

[Close]

BLAST of Lsi04G005480 vs. NCBI nr
Match: XP_022151178.1 (carbonic anhydrase 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 357.8 bits (917), Expect = 1.1e-94
Identity = 196/300 (65.33%), Postives = 213/300 (71.00%), Query Frame = 0


Query: 88  TPFLLEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIE 147
           TP + EDMAQESYEEAIAGL KLLS                                  E
Sbjct: 14  TPKVSEDMAQESYEEAIAGLKKLLS----------------------------------E 73

Query: 148 KADLQDAAAAKIRQITAELAGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKS 207
           KADLQDAAAAKIRQITAEL+ TTAGS+ FDPVDRI+TGFTHFKKSKFETNPDL+GQLAK 
Sbjct: 74  KADLQDAAAAKIRQITAELSATTAGSDSFDPVDRIKTGFTHFKKSKFETNPDLFGQLAKG 133

Query: 208 QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHL 267
           QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIA+MVPP+DK KYSG GAA+EYA+LHL
Sbjct: 134 QSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIANMVPPYDKTKYSGVGAAVEYAILHL 193

Query: 268 KVENIVVIGHSCCGGIKGLMSIPDDGTISRQV----------------AIGVELG-PNLH 327
           KVENIVVIGHSCCGGIKGLMSIPDDGTIS                   + G EL   +  
Sbjct: 194 KVENIVVIGHSCCGGIKGLMSIPDDGTISSDFIENWVKICAPAKTKIQSEGTELSFEDKC 253

Query: 328 TGQNQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNLDFNISTSLAV 371
           T   ++ + +           +RE VVNKKLFIRGAHYNFVSGAFELWNLDF I+ SLAV
Sbjct: 254 TNCEKEAVNVSLGNLLSYPF-VRECVVNKKLFIRGAHYNFVSGAFELWNLDFKITPSLAV 278

[Close]

BLAST of Lsi04G005480 vs. TAIR 10
Match: AT3G01500.2 (carbonic anhydrase 1 )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 162/370 (43.78%), Postives = 206/370 (55.68%), Query Frame = 0


Query: 30  SKFFSKSTAVIKSSFSFTPLKIYSTITFTP--SKSHIFPFCNLDNSKISNLKNDDLNPPP 89
           S FF  S +  +SS     L+  ST+   P  S S      +   S  + ++N+ +   P
Sbjct: 7   SGFFLTSLSPSQSSLQKLSLRTSSTVACLPPASSSSSSSSSSSSRSVPTLIRNEPVFAAP 66

Query: 90  TPFLL----EDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLN 149
            P +     E+M  E+Y+EAI  L KLL                                
Sbjct: 67  APIIAPYWSEEMGTEAYDEAIEALKKLL-------------------------------- 126

Query: 150 VIIEKADLQDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYG 209
             IEK +L+  AAAK+ QITA L  GT++    FDPV+ I+ GF  FKK K+ETNP LYG
Sbjct: 127 --IEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFIKFKKEKYETNPALYG 186

Query: 210 QLAKSQSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEY 269
           +LAK QSPK++VFACSDSRVCPSH+L+FQPG+AFVVRNIA+MVPPFDK KY G GAAIEY
Sbjct: 187 ELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPPFDKVKYGGVGAAIEY 246

Query: 270 AVLHLKVENIVVIGHSCCGGIKGLMSIPDDGT-------------ISRQVAIGVELGPNL 329
           AVLHLKVENIVVIGHS CGGIKGLMS P DG              +  +  +  ELG + 
Sbjct: 247 AVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVISELGDSA 306

Query: 330 HTGQ---------NQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNL 371
              Q         N     L  + F      +RE +V   L ++G +Y+FV GAFELW L
Sbjct: 307 FEDQCGRCEREAVNVSLANLLTYPF------VREGLVKGTLALKGGYYDFVKGAFELWGL 336

[Close]

BLAST of Lsi04G005480 vs. TAIR 10
Match: AT3G01500.3 (carbonic anhydrase 1 )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 162/370 (43.78%), Postives = 206/370 (55.68%), Query Frame = 0


Query: 30  SKFFSKSTAVIKSSFSFTPLKIYSTITFTP--SKSHIFPFCNLDNSKISNLKNDDLNPPP 89
           S FF  S +  +SS     L+  ST+   P  S S      +   S  + ++N+ +   P
Sbjct: 7   SGFFLTSLSPSQSSLQKLSLRTSSTVACLPPASSSSSSSSSSSSRSVPTLIRNEPVFAAP 66

Query: 90  TPFLL----EDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLN 149
            P +     E+M  E+Y+EAI  L KLL                                
Sbjct: 67  APIIAPYWSEEMGTEAYDEAIEALKKLL-------------------------------- 126

Query: 150 VIIEKADLQDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYG 209
             IEK +L+  AAAK+ QITA L  GT++    FDPV+ I+ GF  FKK K+ETNP LYG
Sbjct: 127 --IEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFIKFKKEKYETNPALYG 186

Query: 210 QLAKSQSPKFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEY 269
           +LAK QSPK++VFACSDSRVCPSH+L+FQPG+AFVVRNIA+MVPPFDK KY G GAAIEY
Sbjct: 187 ELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPPFDKVKYGGVGAAIEY 246

Query: 270 AVLHLKVENIVVIGHSCCGGIKGLMSIPDDGT-------------ISRQVAIGVELGPNL 329
           AVLHLKVENIVVIGHS CGGIKGLMS P DG              +  +  +  ELG + 
Sbjct: 247 AVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVISELGDSA 306

Query: 330 HTGQ---------NQDPIRLHRFEFRGQMHKLREAVVNKKLFIRGAHYNFVSGAFELWNL 371
              Q         N     L  + F      +RE +V   L ++G +Y+FV GAFELW L
Sbjct: 307 FEDQCGRCEREAVNVSLANLLTYPF------VREGLVKGTLALKGGYYDFVKGAFELWGL 336

[Close]

BLAST of Lsi04G005480 vs. TAIR 10
Match: AT5G14740.1 (carbonic anhydrase 2 )

HSP 1 Score: 258.1 bits (658), Expect = 1.1e-68
Identity = 148/291 (50.86%), Postives = 176/291 (60.48%), Query Frame = 0


Query: 92  LEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADL 151
           L  M  ESYE+AI  L KLL                                  IEK DL
Sbjct: 70  LRRMGNESYEDAIEALKKLL----------------------------------IEKDDL 129

Query: 152 QDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSP 211
           +D AAAK+++ITAEL A +++ S  FDPV+RI+ GF  FKK K+ETNP LYG+LAK QSP
Sbjct: 130 KDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFVTFKKEKYETNPALYGELAKGQSP 189

Query: 212 KFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVE 271
           K++VFACSDSRVCPSH+L+F PG+AFVVRNIA+MVPPFDK KY+G GAAIEYAVLHLKVE
Sbjct: 190 KYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKVE 249

Query: 272 NIVVIGHSCCGGIKGLMSIPDDGTISRQV----------AIGVELGPNLHTGQNQDPIRL 331
           NIVVIGHS CGGIKGLMS P DG  S             A    L  +  +       R 
Sbjct: 250 NIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVLAESESSAFEDQCGRC 309

Query: 332 HRFEFRGQMHKL------REAVVNKKLFIRGAHYNFVSGAFELWNLDFNIS 366
            R      +  L      RE VV   L ++G +Y+FV+G+FELW L F IS
Sbjct: 310 EREAVNVSLANLLTYPFVREGVVKGTLALKGGYYDFVNGSFELWELQFGIS 326

[Close]

BLAST of Lsi04G005480 vs. TAIR 10
Match: AT5G14740.2 (carbonic anhydrase 2 )

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-68
Identity = 147/288 (51.04%), Postives = 175/288 (60.76%), Query Frame = 0


Query: 95  MAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADLQDA 154
           M  ESYE+AI  L KLL                                  IEK DL+D 
Sbjct: 1   MGNESYEDAIEALKKLL----------------------------------IEKDDLKDV 60

Query: 155 AAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSPKFL 214
           AAAK+++ITAEL A +++ S  FDPV+RI+ GF  FKK K+ETNP LYG+LAK QSPK++
Sbjct: 61  AAAKVKKITAELQAASSSDSKSFDPVERIKEGFVTFKKEKYETNPALYGELAKGQSPKYM 120

Query: 215 VFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVENIV 274
           VFACSDSRVCPSH+L+F PG+AFVVRNIA+MVPPFDK KY+G GAAIEYAVLHLKVENIV
Sbjct: 121 VFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKVENIV 180

Query: 275 VIGHSCCGGIKGLMSIPDDGTISRQV----------AIGVELGPNLHTGQNQDPIRLHRF 334
           VIGHS CGGIKGLMS P DG  S             A    L  +  +       R  R 
Sbjct: 181 VIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVLAESESSAFEDQCGRCERE 240

Query: 335 EFRGQMHKL------REAVVNKKLFIRGAHYNFVSGAFELWNLDFNIS 366
                +  L      RE VV   L ++G +Y+FV+G+FELW L F IS
Sbjct: 241 AVNVSLANLLTYPFVREGVVKGTLALKGGYYDFVNGSFELWELQFGIS 254

[Close]

BLAST of Lsi04G005480 vs. TAIR 10
Match: AT5G14740.3 (carbonic anhydrase 2 )

HSP 1 Score: 256.1 bits (653), Expect = 4.2e-68
Identity = 148/290 (51.03%), Postives = 176/290 (60.69%), Query Frame = 0


Query: 92  LEDMAQESYEEAIAGLAKLLSRPPLIRAAAHHVGPTRIDCPKERSVTVSVLNVIIEKADL 151
           L  M  ESYE+AI  L KLL                                  IEK DL
Sbjct: 70  LRRMGNESYEDAIEALKKLL----------------------------------IEKDDL 129

Query: 152 QDAAAAKIRQITAEL-AGTTAGSNGFDPVDRIRTGFTHFKKSKFETNPDLYGQLAKSQSP 211
           +D AAAK+++ITAEL A +++ S  FDPV+RI+ GF  FKK K+ETNP LYG+LAK QSP
Sbjct: 130 KDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFVTFKKEKYETNPALYGELAKGQSP 189

Query: 212 KFLVFACSDSRVCPSHILNFQPGEAFVVRNIASMVPPFDKKKYSGAGAAIEYAVLHLKVE 271
           K++VFACSDSRVCPSH+L+F PG+AFVVRNIA+MVPPFDK KY+G GAAIEYAVLHLKVE
Sbjct: 190 KYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKVE 249

Query: 272 NIVVIGHSCCGGIKGLMSIPDDGTISRQV----------AIGVELGPNLHTGQNQDPIRL 331
           NIVVIGHS CGGIKGLMS P DG  S             A    L  +  +       R 
Sbjct: 250 NIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVLAESESSAFEDQCGRC 309

Query: 332 HRFEFRGQMHKL-----REAVVNKKLFIRGAHYNFVSGAFELWNLDFNIS 366
            R       + L     RE VV   L ++G +Y+FV+G+FELW L F IS
Sbjct: 310 ERVLNVSLANLLTYPFVREGVVKGTLALKGGYYDFVNGSFELWELQFGIS 325

The following BLAST results are available for this feature:

BLAST of Lsi04G005480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P27141	8.0e-72	46.09	Carbonic anhydrase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P27140	3.1e-68	43.78	Beta carbonic anhydrase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA1...	[more]
P42737	1.6e-67	50.86	Beta carbonic anhydrase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA2...	[more]
P17067	4.2e-65	43.21	Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1	[more]
Q94CE4	2.7e-64	48.64	Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1	[more]

BLAST of Lsi04G005480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SWR5	3.3e-97	69.18	Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3BEI1	8.2e-96	67.46	Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103489011 PE=3 SV=1	[more]
A0A5D3CXD3	8.2e-96	67.46	Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00...	[more]
E5GBJ5	8.2e-96	67.46	Carbonic anhydrase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A6J1DBH1	5.3e-95	65.33	Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111019165 PE=3 SV=1	[more]

BLAST of Lsi04G005480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893413.1	1.3e-98	70.51	carbonic anhydrase 2-like isoform X1 [Benincasa hispida]	[more]
XP_038893414.1	2.4e-97	70.31	carbonic anhydrase 2-like isoform X2 [Benincasa hispida] >XP_038893415.1 carboni...	[more]
KAA0034316.1	6.9e-97	69.18	carbonic anhydrase 2-like [Cucumis melo var. makuwa]	[more]
XP_008446208.1	1.7e-95	67.46	PREDICTED: carbonic anhydrase 2-like [Cucumis melo] >XP_008446210.1 PREDICTED: c...	[more]
XP_022151178.1	1.1e-94	65.33	carbonic anhydrase 2-like isoform X1 [Momordica charantia]	[more]

BLAST of Lsi04G005480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01500.2	2.2e-69	43.78	carbonic anhydrase 1	[more]
AT3G01500.3	2.2e-69	43.78	carbonic anhydrase 1	[more]
AT5G14740.1	1.1e-68	50.86	carbonic anhydrase 2	[more]
AT5G14740.2	1.9e-68	51.04	carbonic anhydrase 2	[more]
AT5G14740.3	4.2e-68	51.03	carbonic anhydrase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001765	Carbonic anhydrase	SMART	SM00947	Pro_CA_2	coord: 205..359 e-value: 3.8E-42 score: 156.0
IPR001765	Carbonic anhydrase	PFAM	PF00484	Pro_CA	coord: 213..308 e-value: 1.3E-27 score: 97.0
IPR036874	Carbonic anhydrase superfamily	GENE3D	3.40.1050.10	Carbonic anhydrase	coord: 171..370 e-value: 2.6E-53 score: 182.6
IPR036874	Carbonic anhydrase superfamily	SUPERFAMILY	53056	beta-carbonic anhydrase, cab	coord: 175..366
None	No IPR available	PANTHER	PTHR11002	CARBONIC ANHYDRASE	coord: 141..297
None	No IPR available	PANTHER	PTHR11002	CARBONIC ANHYDRASE	coord: 332..370
None	No IPR available	PANTHER	PTHR11002:SF67	CARBONIC ANHYDRASE	coord: 141..297
None	No IPR available	PANTHER	PTHR11002:SF67	CARBONIC ANHYDRASE	coord: 332..370
IPR015892	Carbonic anhydrase, prokaryotic-like, conserved site	PROSITE	PS00705	PROK_CO2_ANHYDRASE_2	coord: 261..281
IPR015892	Carbonic anhydrase, prokaryotic-like, conserved site	PROSITE	PS00704	PROK_CO2_ANHYDRASE_1	coord: 217..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G005480.1	Lsi04G005480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015976	carbon utilization
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding