Lsi04G005370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G005370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationchr04: 4965797 .. 4967024 (-)
RNA-Seq ExpressionLsi04G005370
SyntenyLsi04G005370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAAGGATGGGATTTTCCAGAGGAATAATGGAATTGCCCCTTTTGAGCTTCATCTTTTTGTGCATGTTATTTGCAGTCCCTTCTTGTGGTAGATATTACACACCTCCGAACGTCCCACGTTTGACGGATCTTGTTTCTCATGTCTCAATCGATCAATGCTTTGCTAAGATTTTTGGGGCCTCAAATATCCAGCTGAGGAATAATGGGTCCTCTGTCGATCTCACTCTCGACAAAGTTTCTGGTAAATTTCACCTTTGGATCTTCGCATTTCTTTTTGGTCCAATTCCATTTTCATTCATAACTTTTTCTCTGTTCCTTTCTGGAGGTGCTGGTCTGGTCTCGAGAAACAAGTATCATTATGGATTCTTCAGTGCTTCAATTAAGCTCCCATCAGGTCTCACTTCAGGGGTTGTAGTGGCTTTTTATGTAAGTTTTTCTTTTTTTCCCATTTCCATTCTCTGTTTGCCTTACCAGTTTGGTGAATTGCTAACAAGACGAGGGCTGTGTTCAGTTGTCAAATGCAGATGTTTATCCCCATTCTCATGACGAGATCGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATATATGCCAATGGAAGCGTTAAGACTGGAAGAGAAGAAAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCATGATTACACCATCATTTGGAACAACCATCATACAGTGTAAGTGCATGAAATTCAATCAGAATCAAACCAAAAGGCAAAATCATTTTTTTTCTTTTCAAAAAAGGGTCTTTGTATTTCCCTAGTTTTCTAAAAGGTGCCTCGAAATGCAAGCAGGTTTCTGGTGGATAACGTTCCAGTCAGAGAGCTGAGAAACTATGAAGCTTTTTATCCATCAAAGCCCATGTCAGTTTTCGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAATACCCAGTTGACTACAAGCATGCACCATATGTAGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTATATCAACACCAACAGCCACTGTTCCTTCAGGTTCCAAAACCAATGTCTCGAGCCCAGATACTGTGGAGGGTCCAGAATTTATCAAGCTATCACAGCAGCAAATAGATGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAG

mRNA sequence

ATGATAAGGATGGGATTTTCCAGAGGAATAATGGAATTGCCCCTTTTGAGCTTCATCTTTTTGTGCATGTTATTTGCAGTCCCTTCTTGTGGTAGATATTACACACCTCCGAACGTCCCACGTTTGACGGATCTTGTTTCTCATGTCTCAATCGATCAATGCTTTGCTAAGATTTTTGGGGCCTCAAATATCCAGCTGAGGAATAATGGGTCCTCTGTCGATCTCACTCTCGACAAAGTTTCTGGTAAATTTCACCTTTGGATCTTCGCATTTCTTTTTGGTCCAATTCCATTTTCATTCATAACTTTTTCTCTGTTCCTTTCTGGAGGTGCTGGTCTGGTCTCGAGAAACAAGTATCATTATGGATTCTTCAGTGCTTCAATTAAGCTCCCATCAGGTCTCACTTCAGGGGTTGTAGTGGCTTTTTATTTGTCAAATGCAGATGTTTATCCCCATTCTCATGACGAGATCGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATATATGCCAATGGAAGCGTTAAGACTGGAAGAGAAGAAAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCATGATTACACCATCATTTGGAACAACCATCATACAGTGTTTCTGGTGGATAACGTTCCAGTCAGAGAGCTGAGAAACTATGAAGCTTTTTATCCATCAAAGCCCATGTCAGTTTTCGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAATACCCAGTTGACTACAAGCATGCACCATATGTAGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTATATCAACACCAACAGCCACTGTTCCTTCAGGTTCCAAAACCAATGTCTCGAGCCCAGATACTGTGGAGGGTCCAGAATTTATCAAGCTATCACAGCAGCAAATAGATGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAG

Coding sequence (CDS)

ATGATAAGGATGGGATTTTCCAGAGGAATAATGGAATTGCCCCTTTTGAGCTTCATCTTTTTGTGCATGTTATTTGCAGTCCCTTCTTGTGGTAGATATTACACACCTCCGAACGTCCCACGTTTGACGGATCTTGTTTCTCATGTCTCAATCGATCAATGCTTTGCTAAGATTTTTGGGGCCTCAAATATCCAGCTGAGGAATAATGGGTCCTCTGTCGATCTCACTCTCGACAAAGTTTCTGGTAAATTTCACCTTTGGATCTTCGCATTTCTTTTTGGTCCAATTCCATTTTCATTCATAACTTTTTCTCTGTTCCTTTCTGGAGGTGCTGGTCTGGTCTCGAGAAACAAGTATCATTATGGATTCTTCAGTGCTTCAATTAAGCTCCCATCAGGTCTCACTTCAGGGGTTGTAGTGGCTTTTTATTTGTCAAATGCAGATGTTTATCCCCATTCTCATGACGAGATCGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATATATGCCAATGGAAGCGTTAAGACTGGAAGAGAAGAAAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCATGATTACACCATCATTTGGAACAACCATCATACAGTGTTTCTGGTGGATAACGTTCCAGTCAGAGAGCTGAGAAACTATGAAGCTTTTTATCCATCAAAGCCCATGTCAGTTTTCGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAATACCCAGTTGACTACAAGCATGCACCATATGTAGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTATATCAACACCAACAGCCACTGTTCCTTCAGGTTCCAAAACCAATGTCTCGAGCCCAGATACTGTGGAGGGTCCAGAATTTATCAAGCTATCACAGCAGCAAATAGATGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAG

Protein sequence

MIRMGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK
Homology
BLAST of Lsi04G005370 vs. ExPASy Swiss-Prot
Match: Q8LC45 (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana OX=3702 GN=XTH33 PE=1 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 6.6e-105
Identity = 190/327 (58.10%), Postives = 229/327 (70.03%), Query Frame = 0

Query: 21  LCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASNIQLRNNGSSVDLTLDKV 80
           LC L  V S  R +T PNV RLTD  S ++I+  F++ FGA NIQ+  NGS   LTLDK 
Sbjct: 13  LCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLDK- 72

Query: 81  SGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGFFSASIKLPSGLTSGVVV 140
                                      S GAGLVS+NKYHYGFFSA +KLP+G  SGVVV
Sbjct: 73  ---------------------------SSGAGLVSKNKYHYGFFSARLKLPAGFASGVVV 132

Query: 141 AFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKY 200
           AFYLSNA+ YP SHDEIDIELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +
Sbjct: 133 AFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAF 192

Query: 201 HDYTIIWNNHHTVFLVDNVPVRELRNYEAF---YPSKPMSVFVTIWDGSEWATHGGKYPV 260
           HDYT+IWN+HHTVFLVDN+PVR+  N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV
Sbjct: 193 HDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPV 252

Query: 261 DYKHAPYVASFEEMEINGSISTPTATVPSGSKT----NVSSPDTVEGPEFIKLSQQQIDA 320
           +YK+AP+V S  ++E++G      ++  SG  T    ++SS D V+G +F  LS+ QI+A
Sbjct: 253 NYKYAPFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINA 309

Query: 321 MDWARRKLMFYSYCKDTSRYKVLPPEC 341
           MDWARRKLMFYSYC D  RYKV+P EC
Sbjct: 313 MDWARRKLMFYSYCSDKPRYKVMPAEC 309

BLAST of Lsi04G005370 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 128/294 (43.54%), Postives = 183/294 (62.24%), Query Frame = 0

Query: 50  SIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSG 109
           S ++ + ++FG  N+ +  +G SV LTLD+ +                            
Sbjct: 30  SFEESYTQLFGDKNLFVHQDGKSVRLTLDERT---------------------------- 89

Query: 110 GAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKD 169
           G+G VS + Y +GFFSASIKLPS  T+GVVVAFY+SN D+Y  +HDEID E LG+ + K+
Sbjct: 90  GSGFVSNDYYLHGFFSASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKE 149

Query: 170 WVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELR---N 229
           W +QTNIY NGS  +GREE++ LWFDP+  +H Y+I+W++ H +F VDNVP+RE++    
Sbjct: 150 WRVQTNIYGNGSTHSGREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAE 209

Query: 230 YEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPS 289
               +PSKPMS++ TIWDGS+WAT+GGKY V+YK+APY+A F ++ ++G    P    P 
Sbjct: 210 MGGHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP- 269

Query: 290 GSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
             + +  + + +   + I  SQ+    MD  RR+LM YSYC D +RY V   EC
Sbjct: 270 --RCDEGAAEDMRAAQEITPSQR--SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Lsi04G005370 vs. ExPASy Swiss-Prot
Match: Q38909 (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana OX=3702 GN=XTH28 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.1e-65
Identity = 124/295 (42.03%), Postives = 181/295 (61.36%), Query Frame = 0

Query: 49  VSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLS 108
           +  D+ + ++FG  N+ +  +G SV LTLD+ +                           
Sbjct: 29  IQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERT--------------------------- 88

Query: 109 GGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRK 168
            G+G VS + Y +GFFS+SIKLP+  ++GVV+AFYLSN D+Y  +HDEID E LG+ + +
Sbjct: 89  -GSGFVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGR 148

Query: 169 DWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYE 228
           +W IQTNIY NGS   GREE++ LWFDP+  +H Y+I+W+  H +F VDNVP+RE++   
Sbjct: 149 EWRIQTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTA 208

Query: 229 AF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVP 288
           +    +P+KPMS++ TIWDGS+WAT GGKY V+YK+APYV+ F ++ ++G    PT   P
Sbjct: 209 SMGGDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFP 268

Query: 289 SGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
           S     V +          ++++ Q + M+  R+K M YSYC D  RYKV+  EC
Sbjct: 269 SCKDEAVQNLRLAS-----EITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Lsi04G005370 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 2.8e-63
Identity = 121/295 (41.02%), Postives = 173/295 (58.64%), Query Frame = 0

Query: 49  VSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLS 108
           +S ++  + +FG +N+    +  SV L LD+ +                           
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYT--------------------------- 89

Query: 109 GGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRK 168
            G+G +S N Y +GF+S+ IKLP+  T+GVVVAFY SN DV+  +HDE+DIE LG+ K K
Sbjct: 90  -GSGFISSNMYQHGFYSSMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGK 149

Query: 169 DWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYE 228
            W  QTN+Y NGS   GREE++ LWFDPS ++H Y+I+W  H  +F VD+VP+RE+   +
Sbjct: 150 PWRFQTNLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRND 209

Query: 229 AF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVP 288
           A    YP+KPM+++ TIWD S+WAT GGKY  +YK AP+VA F+   ++G    P   VP
Sbjct: 210 AMGADYPAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVP 269

Query: 289 SGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
                +V   D +E  ++  ++  Q  AM   R++ M+YSYC DT RY    PEC
Sbjct: 270 MDCSDSV---DFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Lsi04G005370 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.2e-59
Identity = 121/306 (39.54%), Postives = 174/306 (56.86%), Query Frame = 0

Query: 46  VSHVSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSL 105
           ++ +  D+  + +FG  N+    +  SV L LDK +                        
Sbjct: 35  INPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYT------------------------ 94

Query: 106 FLSGGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHD 165
               G+G +S + Y +GFFS+ IKLP   T+G+VVAFY SN DV+   HDE+DIE LG+ 
Sbjct: 95  ----GSGFISSSMYQHGFFSSLIKLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNL 154

Query: 166 KRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELR 225
           + K W  QTN+Y NGS   GREE++ LWFDPS ++H Y+I+W     +F VD+VP+RE+ 
Sbjct: 155 EGKPWRFQTNMYGNGSTNRGREERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREIL 214

Query: 226 NYEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEING---SIST 285
             E     YP KPMS++ TIWD S WAT GGK+ VDY  +P+V+ F+++ ++G   S S 
Sbjct: 215 RKEEMNGDYPQKPMSLYATIWDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSF 274

Query: 286 PTATVPS--GSKTNVS---SPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYK 341
           P     +  G+  N++   S   +   ++  +S +Q  AM   R + M+YSYC DT RY 
Sbjct: 275 PGENNNNNIGNYNNINCSVSDQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYS 312

BLAST of Lsi04G005370 vs. ExPASy TrEMBL
Match: A0A0A0KQA3 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G602120 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.8e-167
Identity = 290/338 (85.80%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSRGIMELPLLSFIFLCMLF  PSC RYYTPP+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRGIMELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPS+KYHDYTIIWNN+HTVFLVDNVPVRELRN E FYPSKPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TP ATVPS SK NVS PDT EGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPKATVPSSSKANVSGPDTAEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. ExPASy TrEMBL
Match: A0A5D3CYT9 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00930 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 292/338 (86.39%), Postives = 295/338 (87.28%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSR IMELPLLSFIFLCMLFAVPSC RYYT P+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRVIMELPLLSFIFLCMLFAVPSCSRYYTAPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYP SHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPDSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDNVPVRELRN E FYP KPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPLKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TPTATVPSGSK NVS PDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSGSKANVSGPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. ExPASy TrEMBL
Match: E5GBK2 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 292/338 (86.39%), Postives = 295/338 (87.28%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSR IMELPLLSFIFLCMLFAVPSC RYYT P+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRVIMELPLLSFIFLCMLFAVPSCSRYYTAPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYP SHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPDSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDNVPVRELRN E FYP KPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPLKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TPTATVPSGSK NVS PDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSGSKANVSGPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. ExPASy TrEMBL
Match: A0A1S3BEI9 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103489017 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 292/338 (86.39%), Postives = 295/338 (87.28%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSR IMELPLLSFIFLCMLFAVPSC RYYT P+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRVIMELPLLSFIFLCMLFAVPSCSRYYTAPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYP SHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPDSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDNVPVRELRN E FYP KPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPLKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TPTATVPSGSK NVS PDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSGSKANVSGPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. ExPASy TrEMBL
Match: N0DX87 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 284/331 (85.80%), Postives = 288/331 (87.01%), Query Frame = 0

Query: 11  MELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASNIQLRNNG 70
           MELPLLSFIFLCMLF  PSC RYYTPP+VPRLTDLV  VS DQCFAKIFGASNIQLRNNG
Sbjct: 1   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 60

Query: 71  SSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGFFSASIKL 130
           SSVDLTLDKVS                            GAGLVSRNKYHYGFFSASIKL
Sbjct: 61  SSVDLTLDKVS----------------------------GAGLVSRNKYHYGFFSASIKL 120

Query: 131 PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF 190
           PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF
Sbjct: 121 PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF 180

Query: 191 YLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIWDGSEWAT 250
           YLWFDPS+KYHDYTIIWNN+HTVFLVDNVPVRELRN E FYPSKPMSVFVTIWDGSEWAT
Sbjct: 181 YLWFDPSLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWAT 240

Query: 251 HGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEFIKLSQQQ 310
           HGGKYPVDYKHAPY ASFEEMEING I TPTATVPS SK NVS PDT EGPEFIKLSQQQ
Sbjct: 241 HGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSSSKANVSGPDTAEGPEFIKLSQQQ 300

Query: 311 IDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           +DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 VDAMDWARRKLMFYSYCKDTSRYKVLPPECK 303

BLAST of Lsi04G005370 vs. NCBI nr
Match: XP_038892285.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X1 [Benincasa hispida])

HSP 1 Score: 668.3 bits (1723), Expect = 3.5e-188
Identity = 320/338 (94.67%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSRGIMELPLLSFIFLC+LFAVPSC RYYTPP+VPRLTDLV HVSIDQCFAKIFGASN
Sbjct: 1   MGFSRGIMELPLLSFIFLCILFAVPSCARYYTPPSVPRLTDLVPHVSIDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVSGKF+L I AF+FGP  FSFITFSL + GGAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVSGKFYLLILAFIFGPTLFSFITFSLLVFGGAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLP+GLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPAGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDN+PVRELRN EAFYPSKPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNIPVRELRNSEAFYPSKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQIDAMDWARRKLMFYSYCKDTSRY+VLPPECK
Sbjct: 301 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYRVLPPECK 338

BLAST of Lsi04G005370 vs. NCBI nr
Match: XP_038892286.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Benincasa hispida])

HSP 1 Score: 619.0 bits (1595), Expect = 2.4e-173
Identity = 301/338 (89.05%), Postives = 307/338 (90.83%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSRGIMELPLLSFIFLC+LFAVPSC RYYTPP+VPRLTDLV HVSIDQCFAKIFGASN
Sbjct: 1   MGFSRGIMELPLLSFIFLCILFAVPSCARYYTPPSVPRLTDLVPHVSIDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLP+GLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPAGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDN+PVRELRN EAFYPSKPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNIPVRELRNSEAFYPSKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQIDAMDWARRKLMFYSYCKDTSRY+VLPPECK
Sbjct: 301 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYRVLPPECK 310

BLAST of Lsi04G005370 vs. NCBI nr
Match: XP_004135238.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis sativus] >KGN51813.1 hypothetical protein Csa_008560 [Cucumis sativus])

HSP 1 Score: 597.0 bits (1538), Expect = 9.9e-167
Identity = 290/338 (85.80%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSRGIMELPLLSFIFLCMLF  PSC RYYTPP+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRGIMELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPS+KYHDYTIIWNN+HTVFLVDNVPVRELRN E FYPSKPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TP ATVPS SK NVS PDT EGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPKATVPSSSKANVSGPDTAEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. NCBI nr
Match: XP_008446221.1 (PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis melo] >ADN33843.1 xyloglucan endotransglycosylase hydrolase [Cucumis melo subsp. melo] >KAA0034323.1 putative xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis melo var. makuwa] >TYK15596.1 putative xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis melo var. makuwa])

HSP 1 Score: 595.1 bits (1533), Expect = 3.8e-166
Identity = 292/338 (86.39%), Postives = 295/338 (87.28%), Query Frame = 0

Query: 4   MGFSRGIMELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASN 63
           MGFSR IMELPLLSFIFLCMLFAVPSC RYYT P+VPRLTDLV  VS DQCFAKIFGASN
Sbjct: 1   MGFSRVIMELPLLSFIFLCMLFAVPSCSRYYTAPSVPRLTDLVPRVSTDQCFAKIFGASN 60

Query: 64  IQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGF 123
           IQLRNNGSSVDLTLDKVS                            GAGLVSRNKYHYGF
Sbjct: 61  IQLRNNGSSVDLTLDKVS----------------------------GAGLVSRNKYHYGF 120

Query: 124 FSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 183
           FSASIKLPSGLTSGVVVAFYLSNADVYP SHDEIDIELLGHDKRKDWVIQTNIYANGSVK
Sbjct: 121 FSASIKLPSGLTSGVVVAFYLSNADVYPDSHDEIDIELLGHDKRKDWVIQTNIYANGSVK 180

Query: 184 TGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIW 243
           TGREEKFYLWFDPSVKYHDYTIIWNN+HTVFLVDNVPVRELRN E FYP KPMSVFVTIW
Sbjct: 181 TGREEKFYLWFDPSVKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPLKPMSVFVTIW 240

Query: 244 DGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEF 303
           DGSEWATHGGKYPVDYKHAPY ASFEEMEING I TPTATVPSGSK NVS PDTVEGPEF
Sbjct: 241 DGSEWATHGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSGSKANVSGPDTVEGPEF 300

Query: 304 IKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           IKLSQQQ+DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 IKLSQQQVDAMDWARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Lsi04G005370 vs. NCBI nr
Match: CCH26639.1 (putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus])

HSP 1 Score: 585.5 bits (1508), Expect = 3.0e-163
Identity = 284/331 (85.80%), Postives = 288/331 (87.01%), Query Frame = 0

Query: 11  MELPLLSFIFLCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASNIQLRNNG 70
           MELPLLSFIFLCMLF  PSC RYYTPP+VPRLTDLV  VS DQCFAKIFGASNIQLRNNG
Sbjct: 1   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 60

Query: 71  SSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGFFSASIKL 130
           SSVDLTLDKVS                            GAGLVSRNKYHYGFFSASIKL
Sbjct: 61  SSVDLTLDKVS----------------------------GAGLVSRNKYHYGFFSASIKL 120

Query: 131 PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF 190
           PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF
Sbjct: 121 PSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKF 180

Query: 191 YLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYEAFYPSKPMSVFVTIWDGSEWAT 250
           YLWFDPS+KYHDYTIIWNN+HTVFLVDNVPVRELRN E FYPSKPMSVFVTIWDGSEWAT
Sbjct: 181 YLWFDPSLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWAT 240

Query: 251 HGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEFIKLSQQQ 310
           HGGKYPVDYKHAPY ASFEEMEING I TPTATVPS SK NVS PDT EGPEFIKLSQQQ
Sbjct: 241 HGGKYPVDYKHAPYTASFEEMEINGGILTPTATVPSSSKANVSGPDTAEGPEFIKLSQQQ 300

Query: 311 IDAMDWARRKLMFYSYCKDTSRYKVLPPECK 342
           +DAMDWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 VDAMDWARRKLMFYSYCKDTSRYKVLPPECK 303

BLAST of Lsi04G005370 vs. TAIR 10
Match: AT1G10550.1 (xyloglucan:xyloglucosyl transferase 33 )

HSP 1 Score: 382.1 bits (980), Expect = 4.7e-106
Identity = 190/327 (58.10%), Postives = 229/327 (70.03%), Query Frame = 0

Query: 21  LCMLFAVPSCGRYYTPPNVPRLTDLVSHVSIDQCFAKIFGASNIQLRNNGSSVDLTLDKV 80
           LC L  V S  R +T PNV RLTD  S ++I+  F++ FGA NIQ+  NGS   LTLDK 
Sbjct: 13  LCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLDK- 72

Query: 81  SGKFHLWIFAFLFGPIPFSFITFSLFLSGGAGLVSRNKYHYGFFSASIKLPSGLTSGVVV 140
                                      S GAGLVS+NKYHYGFFSA +KLP+G  SGVVV
Sbjct: 73  ---------------------------SSGAGLVSKNKYHYGFFSARLKLPAGFASGVVV 132

Query: 141 AFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKY 200
           AFYLSNA+ YP SHDEIDIELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +
Sbjct: 133 AFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAF 192

Query: 201 HDYTIIWNNHHTVFLVDNVPVRELRNYEAF---YPSKPMSVFVTIWDGSEWATHGGKYPV 260
           HDYT+IWN+HHTVFLVDN+PVR+  N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV
Sbjct: 193 HDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPV 252

Query: 261 DYKHAPYVASFEEMEINGSISTPTATVPSGSKT----NVSSPDTVEGPEFIKLSQQQIDA 320
           +YK+AP+V S  ++E++G      ++  SG  T    ++SS D V+G +F  LS+ QI+A
Sbjct: 253 NYKYAPFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINA 309

Query: 321 MDWARRKLMFYSYCKDTSRYKVLPPEC 341
           MDWARRKLMFYSYC D  RYKV+P EC
Sbjct: 313 MDWARRKLMFYSYCSDKPRYKVMPAEC 309

BLAST of Lsi04G005370 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 258.5 bits (659), Expect = 7.8e-69
Identity = 128/294 (43.54%), Postives = 183/294 (62.24%), Query Frame = 0

Query: 50  SIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLSG 109
           S ++ + ++FG  N+ +  +G SV LTLD+ +                            
Sbjct: 30  SFEESYTQLFGDKNLFVHQDGKSVRLTLDERT---------------------------- 89

Query: 110 GAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKD 169
           G+G VS + Y +GFFSASIKLPS  T+GVVVAFY+SN D+Y  +HDEID E LG+ + K+
Sbjct: 90  GSGFVSNDYYLHGFFSASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKE 149

Query: 170 WVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELR---N 229
           W +QTNIY NGS  +GREE++ LWFDP+  +H Y+I+W++ H +F VDNVP+RE++    
Sbjct: 150 WRVQTNIYGNGSTHSGREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAE 209

Query: 230 YEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVPS 289
               +PSKPMS++ TIWDGS+WAT+GGKY V+YK+APY+A F ++ ++G    P    P 
Sbjct: 210 MGGHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP- 269

Query: 290 GSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
             + +  + + +   + I  SQ+    MD  RR+LM YSYC D +RY V   EC
Sbjct: 270 --RCDEGAAEDMRAAQEITPSQR--SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Lsi04G005370 vs. TAIR 10
Match: AT1G14720.1 (xyloglucan endotransglucosylase/hydrolase 28 )

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-66
Identity = 124/295 (42.03%), Postives = 181/295 (61.36%), Query Frame = 0

Query: 49  VSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLS 108
           +  D+ + ++FG  N+ +  +G SV LTLD+ +                           
Sbjct: 29  IQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERT--------------------------- 88

Query: 109 GGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRK 168
            G+G VS + Y +GFFS+SIKLP+  ++GVV+AFYLSN D+Y  +HDEID E LG+ + +
Sbjct: 89  -GSGFVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGR 148

Query: 169 DWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYE 228
           +W IQTNIY NGS   GREE++ LWFDP+  +H Y+I+W+  H +F VDNVP+RE++   
Sbjct: 149 EWRIQTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTA 208

Query: 229 AF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVP 288
           +    +P+KPMS++ TIWDGS+WAT GGKY V+YK+APYV+ F ++ ++G    PT   P
Sbjct: 209 SMGGDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFP 268

Query: 289 SGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
           S     V +          ++++ Q + M+  R+K M YSYC D  RYKV+  EC
Sbjct: 269 SCKDEAVQNLRLAS-----EITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Lsi04G005370 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 121/295 (41.02%), Postives = 173/295 (58.64%), Query Frame = 0

Query: 49  VSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSLFLS 108
           +S ++  + +FG +N+    +  SV L LD+ +                           
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYT--------------------------- 89

Query: 109 GGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRK 168
            G+G +S N Y +GF+S+ IKLP+  T+GVVVAFY SN DV+  +HDE+DIE LG+ K K
Sbjct: 90  -GSGFISSNMYQHGFYSSMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGK 149

Query: 169 DWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELRNYE 228
            W  QTN+Y NGS   GREE++ LWFDPS ++H Y+I+W  H  +F VD+VP+RE+   +
Sbjct: 150 PWRFQTNLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRND 209

Query: 229 AF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEINGSISTPTATVP 288
           A    YP+KPM+++ TIWD S+WAT GGKY  +YK AP+VA F+   ++G    P   VP
Sbjct: 210 AMGADYPAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVP 269

Query: 289 SGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 341
                +V   D +E  ++  ++  Q  AM   R++ M+YSYC DT RY    PEC
Sbjct: 270 MDCSDSV---DFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Lsi04G005370 vs. TAIR 10
Match: AT4G18990.1 (xyloglucan endotransglucosylase/hydrolase 29 )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 121/306 (39.54%), Postives = 174/306 (56.86%), Query Frame = 0

Query: 46  VSHVSIDQCFAKIFGASNIQLRNNGSSVDLTLDKVSGKFHLWIFAFLFGPIPFSFITFSL 105
           ++ +  D+  + +FG  N+    +  SV L LDK +                        
Sbjct: 35  INPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYT------------------------ 94

Query: 106 FLSGGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHD 165
               G+G +S + Y +GFFS+ IKLP   T+G+VVAFY SN DV+   HDE+DIE LG+ 
Sbjct: 95  ----GSGFISSSMYQHGFFSSLIKLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNL 154

Query: 166 KRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNNHHTVFLVDNVPVRELR 225
           + K W  QTN+Y NGS   GREE++ LWFDPS ++H Y+I+W     +F VD+VP+RE+ 
Sbjct: 155 EGKPWRFQTNMYGNGSTNRGREERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREIL 214

Query: 226 NYEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASFEEMEING---SIST 285
             E     YP KPMS++ TIWD S WAT GGK+ VDY  +P+V+ F+++ ++G   S S 
Sbjct: 215 RKEEMNGDYPQKPMSLYATIWDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSF 274

Query: 286 PTATVPS--GSKTNVS---SPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYK 341
           P     +  G+  N++   S   +   ++  +S +Q  AM   R + M+YSYC DT RY 
Sbjct: 275 PGENNNNNIGNYNNINCSVSDQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LC456.6e-10558.10Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha... [more]
Q8LDS21.1e-6743.54Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q389095.1e-6542.03Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha... [more]
Q389082.8e-6341.02Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8L7H33.2e-5939.54Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A0A0KQA34.8e-16785.80Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G60... [more]
A0A5D3CYT91.8e-16686.39Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695... [more]
E5GBK21.8e-16686.39Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo subsp. melo OX=412675 ... [more]
A0A1S3BEI91.8e-16686.39Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10348901... [more]
N0DX871.4e-16385.80Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE... [more]
Match NameE-valueIdentityDescription
XP_038892285.13.5e-18894.67probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X1 [Beninc... [more]
XP_038892286.12.4e-17389.05probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Beninc... [more]
XP_004135238.19.9e-16785.80probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis sativus] ... [more]
XP_008446221.13.8e-16686.39PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumi... [more]
CCH26639.13.0e-16385.80putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G10550.14.7e-10658.10xyloglucan:xyloglucosyl transferase 33 [more]
AT2G01850.17.8e-6943.54endoxyloglucan transferase A3 [more]
AT1G14720.13.6e-6642.03xyloglucan endotransglucosylase/hydrolase 28 [more]
AT1G32170.12.0e-6441.02xyloglucan endotransglucosylase/hydrolase 30 [more]
AT4G18990.12.3e-6039.54xyloglucan endotransglucosylase/hydrolase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 305..340
e-value: 4.4E-14
score: 52.1
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 98..341
e-value: 2.3E-93
score: 310.5
coord: 18..101
e-value: 0.0033
score: 14.4
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 56..260
e-value: 6.0E-47
score: 159.4
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 32..268
score: 25.476505
NoneNo IPR availableGENE3D2.60.120.200coord: 36..340
e-value: 4.9E-84
score: 283.9
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 101..341
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 13..82
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 13..82
coord: 101..341
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 108..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G005370.1Lsi04G005370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0009831 plant-type cell wall modification involved in multidimensional cell growth
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0005887 integral component of plasma membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity