Lsi04G005100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G005100
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 4
Locationchr04: 4748600 .. 4751807 (+)
RNA-Seq ExpressionLsi04G005100
SyntenyLsi04G005100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCTTTCGGAGAGGACCAGTCTTTCTTTCTTACCACTCCTTATCTTAGTGATTTTCTTGGATTGGGAATTTTTTGTTTGCTTGTGTAGTCCTAAAAAAAAAAACTATCCGCCGAAGACTCAAATGGGCACGAGAAGAAACTGTTCATCAATTGTGTACAAATAGAAGTTAGTTTCGATTTACTACAACGTATTTCTCTCCCATATGGAGTTTCTGTACATAATTATCACTTCCATTTTTTTGCCTTTAAGCTTCTCTCTTAACCTTGAAGCTACTCTCCATGGAACCACACCGAGTTCTCTTCAAACCAATCATAATTCTTCACTCAGACACCAAATTCTGGACCTTGCCAATCATCCCACCACTGTAAATTGGATGAAGACCATAAGGAGAAAAATCCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAAGCTTATTAGACAGGAGCTTGACAATCTCCACGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCGTAGTGGCTTTCGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGTCAGATTTTGGATAATTTCATATTTCTCGTTCCTCTCCCATTAAATGCTATCTTCTGCTCGATGAAATGACCCAATGAACTTCGTTGGTAATTTTTCATTTCCATTCTTGAATCCGGCTTAATGGGAATCTTCTGTTTGTTCTCATCAGTTTATTAATCGCATCTTTTTTTTTCTTTTTTTGATATGACCGACAAAATTGGATTATGAATGCACCCTGGAAGAAATGCAAATTCAAAGTCTAGAAAAGAACTAAAGATGTCTAAGACATGACAGAATTGTTAGTTTAACAAACAACTTAATAAATCTCATAGATAGTCTATGATTTGATATAGTTCCTACCATAGAAACTATTTAGGATGGTTTTATAAAACCACCCAGACTAAATTCTATAGAGAACAAATTCATTATTTAACATTTTTTCAGAGGGTGTCAGATGTACCAGTGTCTGTACGATAAGTAATGACTAGCCATTGAGATTATAGATTAAAAAAATAATCTTTCTCATTTTACTGTGGAACTGGGGCATATAATGATGTACAGTGTAATGCAGCAAGAGAAAGAAGTTGTGAGGTTTTCAAATATAAGATGGGTGAAAATCTAAAATCAAGACAGTTTTGTTTTTAATTCTAAATTGATTTTGATATAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTTGTGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAGATTCTGAATCAACTGCGACAGAAATTACAGGTTCTCAAAATACATTGCAACTACTCCCCACTTCCAATAACTTTCTGATAATTAATCTGTTCGTAAAGGCGATGATTATGACTCAATCTTGTATACCTTTTCTTGTTAATATGTACATTTCTCTTCCATGCAGGGCACTGTTGTTCTTATATTTCAGCCAGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGTCCTTGATGGTGTAGAAGCTATATTTGGGCTGCACCTTGTGCATAAATATCCAGTCGGCATTGTCGCTTCACGGCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCCATTGATCCTATTTTGGCAGCTTCAGCAGCAATTATTAGTTTGCAAAGCATTGTCTCAAGAGAGATAGACCCTTTAGATTCTCAGGTACTTTGTTTCAGATATGAAAAAGTCTCTGGCCGGATCTGATCATACAAACAAAACTGTCTGTTTCTTTTCATCTTAAACAAGAGTTGAAAGTTGCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAGCTGCACTCAATGTCATCCCGGACTCGGCGACTATTGTCGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGGTAAGTCCGAGTACAGGGTCTTCTACAAGACAGAAGAATTGTCATTTGTGACTGAACTTTTATTGTTCATTTCTTGTGACAGATCATAAACGGGCAGGCAGCGGTACATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCAACTATAAATGATGAACAAATTTATGAACACGTTCGGCGTGTCTCAATGGAAATTGTAGGAAAAGAAAAAACAGAAGTATCTCCAAACTTTATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAAAGTGCCAGGATCTTTCTTATTTCTAGGCACATATAATGAGAGGATTGGAGCTATACATCCTCCACACAGCCCTCACTATAAAATTGATGAGAATGTGCTTCCTATTGGTGCAGCAATTCATGCAGCACTTGCATATTCTTATATCTTGAATTCATCATCAACATCCCATTCTTTCTCTGACCACTGAATAATTCAGTGCATTCTTATAAGTACTCAGTCTTTTTTCCCCCCTATTCTACAAACTTGAATAGGGAAATTTCAAGATCCGGTGGGGTTTAGTACAAAGTATGATTGGTTTTTCCTTATTACAAGAGAGTTTTGCTTTCAGTTTCTTTTAGTCAATGTCGTTCCAATAAGTCATTTGGATTGTGGAATATTATTATACATATCAAAAGAAAACCAACAAACGAAGCCTAGGTGAAGAGGAGAAGGATAACAACATTCGTAGAGATGAAACAACACCAAGAAGACAACAGAAAAGAAAGAGAGCTATGGAATACATAACATCTCAGATTCTTTCCTTGACAATGAACATGACAAGAAGGATAAACAGGAGATCAAGCAGCAACGATGGACTGGTAAAGCTGCTGGTCGAGCTGAGGGACGTCATGTTTCACTTATAAATTTAACTTTCATAGGTTAAATTCATGTGTTAAATTTGTAATTTCTTCCGTTTGAATGAGTTAGGTTTGATTTTAGTTAAATAATAACTTTATTGAGTTAAATTTGATAAATTTTGTATTTATTTTATTTTATTTTTTATTTTGGAGAGTTACTAAAGGCTTGTTTGATAAAGAATCTAAAACAAGGAAATTGAAAAGGAGGGATTCAATGAAAACATATTGTATTTCATGGTTTGATTCAGAAATTGAATTCTAATTTAAACAA

mRNA sequence

AAGCTTTCGGAGAGGACCAGTCTTTCTTTCTTACCACTCCTTATCTTAGTGATTTTCTTGGATTGGGAATTTTTTGTTTGCTTGTGTAGTCCTAAAAAAAAAAACTATCCGCCGAAGACTCAAATGGGCACGAGAAGAAACTGTTCATCAATTGTGTACAAATAGAAGTTAGTTTCGATTTACTACAACGTATTTCTCTCCCATATGGAGTTTCTGTACATAATTATCACTTCCATTTTTTTGCCTTTAAGCTTCTCTCTTAACCTTGAAGCTACTCTCCATGGAACCACACCGAGTTCTCTTCAAACCAATCATAATTCTTCACTCAGACACCAAATTCTGGACCTTGCCAATCATCCCACCACTGTAAATTGGATGAAGACCATAAGGAGAAAAATCCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAAGCTTATTAGACAGGAGCTTGACAATCTCCACGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCGTAGTGGCTTTCGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTTGTGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAGATTCTGAATCAACTGCGACAGAAATTACAGGGCACTGTTGTTCTTATATTTCAGCCAGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGTCCTTGATGGTGTAGAAGCTATATTTGGGCTGCACCTTGTGCATAAATATCCAGTCGGCATTGTCGCTTCACGGCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCCATTGATCCTATTTTGGCAGCTTCAGCAGCAATTATTAGTTTGCAAAGCATTGTCTCAAGAGAGATAGACCCTTTAGATTCTCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAGCTGCACTCAATGTCATCCCGGACTCGGCGACTATTGTCGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGATCATAAACGGGCAGGCAGCGGTACATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCAACTATAAATGATGAACAAATTTATGAACACGTTCGGCGTGTCTCAATGGAAATTGTAGGAAAAGAAAAAACAGAAGTATCTCCAAACTTTATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAAAGTGCCAGGATCTTTCTTATTTCTAGGCACATATAATGAGAGGATTGGAGCTATACATCCTCCACACAGCCCTCACTATAAAATTGATGAGAATGTGCTTCCTATTGGTGCAGCAATTCATGCAGCACTTGCATATTCTTATATCTTGAATTCATCATCAACATCCCATTCTTTCTCTGACCACTGAATAATTCAGTGCATTCTTATAAGTACTCAGTCTTTTTTCCCCCCTATTCTACAAACTTGAATAGGGAAATTTCAAGATCCGGTGGGGTTTAGTACAAAGTATGATTGGTTTTTCCTTATTACAAGAGAGTTTTGCTTTCAGTTTCTTTTAGTCAATGTCGTTCCAATAAGTCATTTGGATTGTGGAATATTATTATACATATCAAAAGAAAACCAACAAACGAAGCCTAGGTGAAGAGGAGAAGGATAACAACATTCGTAGAGATGAAACAACACCAAGAAGACAACAGAAAAGAAAGAGAGCTATGGAATACATAACATCTCAGATTCTTTCCTTGACAATGAACATGACAAGAAGGATAAACAGGAGATCAAGCAGCAACGATGGACTGGTAAAGCTGCTGGTCGAGCTGAGGGACGTCATGTTTCACTTATAAATTTAACTTTCATAGGTTAAATTCATGTGTTAAATTTGTAATTTCTTCCGTTTGAATGAGTTAGGTTTGATTTTAGTTAAATAATAACTTTATTGAGTTAAATTTGATAAATTTTGTATTTATTTTATTTTATTTTTTATTTTGGAGAGTTACTAAAGGCTTGTTTGATAAAGAATCTAAAACAAGGAAATTGAAAAGGAGGGATTCAATGAAAACATATTGTATTTCATGGTTTGATTCAGAAATTGAATTCTAATTTAAACAA

Coding sequence (CDS)

ATGGAGTTTCTGTACATAATTATCACTTCCATTTTTTTGCCTTTAAGCTTCTCTCTTAACCTTGAAGCTACTCTCCATGGAACCACACCGAGTTCTCTTCAAACCAATCATAATTCTTCACTCAGACACCAAATTCTGGACCTTGCCAATCATCCCACCACTGTAAATTGGATGAAGACCATAAGGAGAAAAATCCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAAGCTTATTAGACAGGAGCTTGACAATCTCCACGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCGTAGTGGCTTTCGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTTGTGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAGATTCTGAATCAACTGCGACAGAAATTACAGGGCACTGTTGTTCTTATATTTCAGCCAGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGTCCTTGATGGTGTAGAAGCTATATTTGGGCTGCACCTTGTGCATAAATATCCAGTCGGCATTGTCGCTTCACGGCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCCATTGATCCTATTTTGGCAGCTTCAGCAGCAATTATTAGTTTGCAAAGCATTGTCTCAAGAGAGATAGACCCTTTAGATTCTCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAGCTGCACTCAATGTCATCCCGGACTCGGCGACTATTGTCGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGATCATAAACGGGCAGGCAGCGGTACATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCAACTATAAATGATGAACAAATTTATGAACACGTTCGGCGTGTCTCAATGGAAATTGTAGGAAAAGAAAAAACAGAAGTATCTCCAAACTTTATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAAAGTGCCAGGATCTTTCTTATTTCTAGGCACATATAATGAGAGGATTGGAGCTATACATCCTCCACACAGCCCTCACTATAAAATTGATGAGAATGTGCTTCCTATTGGTGCAGCAATTCATGCAGCACTTGCATATTCTTATATCTTGAATTCATCATCAACATCCCATTCTTTCTCTGACCACTGA

Protein sequence

MEFLYIIITSIFLPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALAYSYILNSSSTSHSFSDH
Homology
BLAST of Lsi04G005100 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-130
Identity = 231/391 (59.08%), Postives = 290/391 (74.17%), Query Frame = 0

Query: 45  ILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVA 104
           +L  A       WM  +RR+IHENPEL +EEF TS+L+R+ELD L + YR P A TGVVA
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 105 FVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLR 164
            VG+G PPFVALRADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 165 QKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAG 224
            +L+GTVVL+FQPAEE GGGAK MI++G ++ +EAIFG+H+    P+G+VASRPG  +AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 225 CGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGA 284
            G F+A I GKGGHAA+P H+IDPILAAS  I+SLQ +VSRE DPLDSQVV+V   Q G 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 285 ALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPT 344
           A NVIPDS TI GTFRAF K+SFN L+ RIEE+I  QA+V RC A +DFL K+ P  PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 345 INDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGS-FLFLGTYNERIGAI 404
           IN   +++   +V+ E+VG +        MG+EDFAF++D +P + + FLG YNE  G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 405 HPPHSPHYKIDENVLPIGAAIHAALAYSYIL 435
            P HSP++ I+E+ LP GAA+ A+LA  Y+L
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLATRYLL 426

BLAST of Lsi04G005100 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-130
Identity = 231/391 (59.08%), Postives = 290/391 (74.17%), Query Frame = 0

Query: 45  ILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVA 104
           +L  A       WM  +RR+IHENPEL +EEF TS+L+R+ELD L + YR P A TGVVA
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 105 FVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLR 164
            VG+G PPFVALRADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 165 QKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAG 224
            +L+GTVVL+FQPAEE GGGAK MI++G ++ +EAIFG+H+    P+G+VASRPG  +AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 225 CGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGA 284
            G F+A I GKGGHAA+P H+IDPILAAS  I+SLQ +VSRE DPLDSQVV+V   Q G 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 285 ALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPT 344
           A NVIPDS TI GTFRAF K+SFN L+ RIEE+I  QA+V RC A +DFL K+ P  PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 345 INDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGS-FLFLGTYNERIGAI 404
           IN   +++   +V+ E+VG +        MG+EDFAF++D +P + + FLG YNE  G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 405 HPPHSPHYKIDENVLPIGAAIHAALAYSYIL 435
            P HSP++ I+E+ LP GAA+ A+LA  Y+L
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLAARYLL 426

BLAST of Lsi04G005100 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 2.2e-129
Identity = 236/431 (54.76%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 10  SIFLPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENP 69
           S F  +SF L L   L   T  S  +N  S +  + L LA      +WM  IRR+IHENP
Sbjct: 2   SFFKWVSFVLILH--LLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENP 61

Query: 70  ELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELV 129
           EL +EE ETSKL+R EL+ + VSY++PVA TGVV +VG+G  PFVALRADMDAL ++E+V
Sbjct: 62  ELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMV 121

Query: 130 EWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMI 189
           EWEHKSKV GKMHAC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK ++
Sbjct: 122 EWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIV 181

Query: 190 NEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPI 249
             GVL+ V AIFGLH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDPI
Sbjct: 182 EAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPI 241

Query: 250 LAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNA 309
           LAAS  I+SLQ +VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  
Sbjct: 242 LAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQ 301

Query: 310 LRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEV 369
           L+ RIE++I  QA+V+ C A +DF+ +E P  PPT+ND+ +++  + VS +++G E    
Sbjct: 302 LKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVE 361

Query: 370 SPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALA 429
               MGSEDF+F+   +PG F F+G  N+    +  PHSP+++++E +LP GA++HA++A
Sbjct: 362 MQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMA 421

Query: 430 YSYILNSSSTS 441
             Y+L   +++
Sbjct: 422 TRYLLELKAST 430

BLAST of Lsi04G005100 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 448.0 bits (1151), Expect = 1.3e-124
Identity = 232/426 (54.46%), Postives = 300/426 (70.42%), Query Frame = 0

Query: 13  LPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENPELA 72
           L L+F L L   L  ++ S       S ++ ++L+ A  P   +WM  IRRKIHENPEL 
Sbjct: 7   LSLTFQL-LLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELG 66

Query: 73  FEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWE 132
           +EE ETSKLIR EL+ + + YR+PVA TGV+ ++G+G PPFVALRADMDALPI+E VEWE
Sbjct: 67  YEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWE 126

Query: 133 HKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMINEG 192
           HKSK+ GKMHAC HD HV MLLGA KIL++ R  LQGTVVLIFQPAEE   GAK M  EG
Sbjct: 127 HKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEG 186

Query: 193 VLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPILAA 252
            L  VEAIFG+HL  + P G  ASR G FLAG G F+A I GKGGHAAIPQH+IDP++AA
Sbjct: 187 ALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAA 246

Query: 253 SAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNALRD 312
           S+ ++SLQ +VSRE DPLDS+VV+V+ V  G A NVIPDS TI GT RAF+   F  L+ 
Sbjct: 247 SSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQ 306

Query: 313 RIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEVSPN 372
           R++E+I  QAAVHRC A ++        +PPT+N++ +Y+  ++V  +++G+E    +  
Sbjct: 307 RVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAP 366

Query: 373 FMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALAYSY 432
            MGSEDF++F++ +PG F  LG  +E  G     HSP Y+I+E+VLP GAAIHA++A  Y
Sbjct: 367 VMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQY 426

Query: 433 ILNSSS 439
           +   +S
Sbjct: 427 LKEKAS 428

BLAST of Lsi04G005100 vs. ExPASy Swiss-Prot
Match: Q5N8F2 (IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL2 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.1e-123
Identity = 225/392 (57.40%), Postives = 278/392 (70.92%), Query Frame = 0

Query: 45  ILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVA 104
           +LD A  P    WM  +R  IHE PELAFEE ETS+L+R ELD + V+YR PVAGTGVVA
Sbjct: 50  VLDRARRPEFAAWMAGVRWAIHERPELAFEEIETSRLVRAELDAMGVAYRHPVAGTGVVA 109

Query: 105 FVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLR 164
            VG+G PPFVALRADMDALP++E V+WEHKSKV  KMHAC HDAH  MLLGA +IL + R
Sbjct: 110 TVGTGRPPFVALRADMDALPMQEEVQWEHKSKVAMKMHACGHDAHTTMLLGAARILQERR 169

Query: 165 QKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAG 224
            +LQGTVVL+FQP EE G GA+ M+  G +D VEAIFG H+  + P G+V SRPG  LAG
Sbjct: 170 HELQGTVVLLFQPGEEVGTGARRMVEAGAVDNVEAIFGFHVSVELPTGVVGSRPGPLLAG 229

Query: 225 CGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGA 284
           CG F+A I GKGGHAA P  S+DPILAAS  +++LQ +VSRE DPL++QVV+V    AG 
Sbjct: 230 CGFFEAVITGKGGHAAHPHASVDPILAASTVVLALQGLVSREADPLEAQVVTVTRFLAGD 289

Query: 285 ALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPT 344
           ALNVIP+S TI GTFR FS + F  L+ RIEE+I  Q+AV+RC A +DF     P +PPT
Sbjct: 290 ALNVIPESITIGGTFRVFSNEGFLRLKRRIEEVIVAQSAVYRCAAAVDFHAGGRPLLPPT 349

Query: 345 INDEQIYEHVRRVSMEIVGKEKTEVS--PNFMGSEDFAFFSDKVPGS-FLFLGTYNERIG 404
           IN   ++ H + V+ E +G     +      MGSEDFA FS+ VP S F F+G  NE  G
Sbjct: 350 INSAALHAHFQAVAAETLGASAAVLGAMEPCMGSEDFAVFSEAVPASHFYFVGVRNEAEG 409

Query: 405 AIHPPHSPHYKIDENVLPIGAAIHAALAYSYI 434
            +H  HSPH+++D+  LP GAA+HA+LA  Y+
Sbjct: 410 LVHLAHSPHFRVDDAALPYGAALHASLAMRYL 441

BLAST of Lsi04G005100 vs. ExPASy TrEMBL
Match: A0A5A7ST02 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003470 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.3e-230
Identity = 410/448 (91.52%), Postives = 427/448 (95.31%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPLSFSLNLE+  L+ TT  SL TNH SSLRHQILDLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VHKYPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKE+PTIPP INDE+IYEHVRRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP+ MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           PIGAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lsi04G005100 vs. ExPASy TrEMBL
Match: A0A5D3CVM4 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00690 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.0e-229
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPLSFSLNLE+  L+ TT  SL TNH SSLRHQILDLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VHKYPVG+VASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKE+PTIPP INDE+IYEHVRRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP+ MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           PIGAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lsi04G005100 vs. ExPASy TrEMBL
Match: A0A0A0KQC3 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.8e-229
Identity = 405/448 (90.40%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPL+FSLNLE+  LHGTT  SL TNH SSL HQI+DLANHPT VNWM
Sbjct: 35  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 94

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 95  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 154

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 155 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 214

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VH+YPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 215 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 274

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 275 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 334

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKEHPTIPP +NDE+IYEHVRRVS
Sbjct: 335 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 394

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP  MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 395 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 454

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           P+GAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 455 PLGAAIHAAVAYSYLLNSSSTSHSFFDH 482

BLAST of Lsi04G005100 vs. ExPASy TrEMBL
Match: A0A1S3BE49 (LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103489042 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.4e-227
Identity = 406/448 (90.62%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPLSFSLNLE+  L+ TT  SL TNH SSLRHQILDLANHPTTVNWM
Sbjct: 1   MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 60

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VHKYPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKE+PTIPP INDE+IYEHVRRVS
Sbjct: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 360

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEK +     MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL
Sbjct: 361 MEIVGKEKQKYLQAXMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           PIGAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 448

BLAST of Lsi04G005100 vs. ExPASy TrEMBL
Match: A0A6J1FSW3 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111446993 PE=3 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.6e-215
Identity = 380/441 (86.17%), Postives = 409/441 (92.74%), Query Frame = 0

Query: 1   MEFLY-IIITSIFLPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMK 60
           ME LY +II SIF        LEA +HGTT SS+QT+ NSSLRHQIL+LAN PTT NWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRAD 120
           TIRRKIHENPELA+EEFETS+LIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKIL+QLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMI EGVLDGVE I GLH++H+YPVG+VASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTF 300
           AIP HSIDP+LAAS AI+SLQ+IVSREIDPLDSQVVSVAMVQAG ALNVIPDS TI GTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSM 360
           RAF KKSFNALRDRIEEIINGQAAVHRCT EIDFLGKEHPTIPPTIN+E++Y+H RRVS+
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLP 420
           EIVGKE T+VSPNFMGSEDFAFFSD+VPGSF+FLGTYNERIGAI+PPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAALAYSYILNSSSTS 441
           IGAAIHAA AYSY+LNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lsi04G005100 vs. NCBI nr
Match: XP_038893400.1 (IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida])

HSP 1 Score: 823.9 bits (2127), Expect = 6.5e-235
Identity = 417/446 (93.50%), Postives = 425/446 (95.29%), Query Frame = 0

Query: 1   MEFLYIIITSIFLPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKT 60
           MEFLYIIITSIFLPL+FSLN EATLH TTP SLQTNH+SSLRHQILD ANHPTTVNWMKT
Sbjct: 1   MEFLYIIITSIFLPLTFSLNFEATLHVTTPISLQTNHDSSLRHQILDFANHPTTVNWMKT 60

Query: 61  IRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADM 120
           IRRKIHENPELAFEEFETS+LIR ELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRADM
Sbjct: 61  IRRKIHENPELAFEEFETSRLIRHELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRADM 120

Query: 121 DALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEE 180
           DALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGA KILNQLRQKLQGTVVLIFQPAEE
Sbjct: 121 DALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGAAKILNQLRQKLQGTVVLIFQPAEE 180

Query: 181 RGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAA 240
           RG GAKDMI EGVLDGVEAIF LHLVHKYPVG+VASR GEFLAGCGSFK KIKGKGGHAA
Sbjct: 181 RGRGAKDMIKEGVLDGVEAIFALHLVHKYPVGVVASRAGEFLAGCGSFKVKIKGKGGHAA 240

Query: 241 IPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFR 300
           IPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIPDSATI GTFR
Sbjct: 241 IPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTFR 300

Query: 301 AFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSME 360
           AFSKKSFNALRDRIEE+INGQAAVHRCTAEIDFLGKEHPTIPPTINDE+IYEHVRRVSME
Sbjct: 301 AFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRRVSME 360

Query: 361 IVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPI 420
           IVGKEK E SP+ MGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPI
Sbjct: 361 IVGKEKIEESPSLMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPI 420

Query: 421 GAAIHAALAYSYILNSSSTSHSFSDH 447
           GAAIHAA AYSY+LNSSSTSHSF DH
Sbjct: 421 GAAIHAAFAYSYLLNSSSTSHSFFDH 446

BLAST of Lsi04G005100 vs. NCBI nr
Match: KAA0034344.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 807.7 bits (2085), Expect = 4.8e-230
Identity = 410/448 (91.52%), Postives = 427/448 (95.31%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPLSFSLNLE+  L+ TT  SL TNH SSLRHQILDLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VHKYPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKE+PTIPP INDE+IYEHVRRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP+ MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           PIGAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lsi04G005100 vs. NCBI nr
Match: TYK15575.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 804.7 bits (2077), Expect = 4.1e-229
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPLSFSLNLE+  L+ TT  SL TNH SSLRHQILDLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VHKYPVG+VASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKE+PTIPP INDE+IYEHVRRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP+ MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           PIGAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lsi04G005100 vs. NCBI nr
Match: XP_004135471.3 (IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 hypothetical protein Csa_009018 [Cucumis sativus])

HSP 1 Score: 803.1 bits (2073), Expect = 1.2e-228
Identity = 405/448 (90.40%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPL+FSLNLE+  LHGTT  SL TNH SSL HQI+DLANHPT VNWM
Sbjct: 38  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 97

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 98  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 157

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 158 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 217

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VH+YPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 218 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 277

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGT 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG ALNVIP+SATI GT
Sbjct: 278 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 337

Query: 301 FRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVS 360
           FRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKEHPTIPP +NDE+IYEHVRRVS
Sbjct: 338 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 397

Query: 361 MEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420
           MEIVGKEKT+VSP  MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 398 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 457

Query: 421 PIGAAIHAALAYSYILNSSSTSHSFSDH 447
           P+GAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 458 PLGAAIHAAVAYSYLLNSSSTSHSFFDH 485

BLAST of Lsi04G005100 vs. NCBI nr
Match: XP_031741348.1 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 798.1 bits (2060), Expect = 3.8e-227
Identity = 405/450 (90.00%), Postives = 424/450 (94.22%), Query Frame = 0

Query: 1   MEFLYII-ITSIFLPLSFSLNLEA-TLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWM 60
           MEFLYI+   SIFLPL+FSLNLE+  LHGTT  SL TNH SSL HQI+DLANHPT VNWM
Sbjct: 38  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 97

Query: 61  KTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETS+LIRQELDNL VSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 98  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 157

Query: 121 DMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV GKMHACSHDAHVAMLLGATKILNQLR KLQGTVVL+FQPA
Sbjct: 158 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 217

Query: 181 EERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMINEG LDGVEAIFGLH+VH+YPVG+VASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 218 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 277

Query: 241 AAIPQHSIDPILAASAAIISLQSIVSREIDPLDS--QVVSVAMVQAGAALNVIPDSATIV 300
           AAIPQ SIDPILAASAAIISLQSIVSREIDPLDS  QVVSVAMVQAG ALNVIP+SATI 
Sbjct: 278 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQLQVVSVAMVQAGTALNVIPESATIA 337

Query: 301 GTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRR 360
           GTFRAFSKKSFNALRDRIEE+INGQA VHRCTAEIDFLGKEHPTIPP +NDE+IYEHVRR
Sbjct: 338 GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRR 397

Query: 361 VSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDEN 420
           VSMEIVGKEKT+VSP  MGSEDFAFF+DKVPGSFLFLGTYNERIGAIHPPHSP YKIDEN
Sbjct: 398 VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDEN 457

Query: 421 VLPIGAAIHAALAYSYILNSSSTSHSFSDH 447
           VLP+GAAIHAA+AYSY+LNSSSTSHSF DH
Sbjct: 458 VLPLGAAIHAAVAYSYLLNSSSTSHSFFDH 487

BLAST of Lsi04G005100 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 463.8 bits (1192), Expect = 1.6e-130
Identity = 236/431 (54.76%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 10  SIFLPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENP 69
           S F  +SF L L   L   T  S  +N  S +  + L LA      +WM  IRR+IHENP
Sbjct: 2   SFFKWVSFVLILH--LLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENP 61

Query: 70  ELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELV 129
           EL +EE ETSKL+R EL+ + VSY++PVA TGVV +VG+G  PFVALRADMDAL ++E+V
Sbjct: 62  ELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMV 121

Query: 130 EWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMI 189
           EWEHKSKV GKMHAC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK ++
Sbjct: 122 EWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIV 181

Query: 190 NEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPI 249
             GVL+ V AIFGLH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDPI
Sbjct: 182 EAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPI 241

Query: 250 LAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNA 309
           LAAS  I+SLQ +VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  
Sbjct: 242 LAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQ 301

Query: 310 LRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEV 369
           L+ RIE++I  QA+V+ C A +DF+ +E P  PPT+ND+ +++  + VS +++G E    
Sbjct: 302 LKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVE 361

Query: 370 SPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALA 429
               MGSEDF+F+   +PG F F+G  N+    +  PHSP+++++E +LP GA++HA++A
Sbjct: 362 MQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMA 421

Query: 430 YSYILNSSSTS 441
             Y+L   +++
Sbjct: 422 TRYLLELKAST 430

BLAST of Lsi04G005100 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 448.0 bits (1151), Expect = 9.1e-126
Identity = 232/426 (54.46%), Postives = 300/426 (70.42%), Query Frame = 0

Query: 13  LPLSFSLNLEATLHGTTPSSLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENPELA 72
           L L+F L L   L  ++ S       S ++ ++L+ A  P   +WM  IRRKIHENPEL 
Sbjct: 7   LSLTFQL-LLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELG 66

Query: 73  FEEFETSKLIRQELDNLHVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWE 132
           +EE ETSKLIR EL+ + + YR+PVA TGV+ ++G+G PPFVALRADMDALPI+E VEWE
Sbjct: 67  YEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWE 126

Query: 133 HKSKVVGKMHACSHDAHVAMLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMINEG 192
           HKSK+ GKMHAC HD HV MLLGA KIL++ R  LQGTVVLIFQPAEE   GAK M  EG
Sbjct: 127 HKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEG 186

Query: 193 VLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPILAA 252
            L  VEAIFG+HL  + P G  ASR G FLAG G F+A I GKGGHAAIPQH+IDP++AA
Sbjct: 187 ALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAA 246

Query: 253 SAAIISLQSIVSREIDPLDSQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNALRD 312
           S+ ++SLQ +VSRE DPLDS+VV+V+ V  G A NVIPDS TI GT RAF+   F  L+ 
Sbjct: 247 SSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQ 306

Query: 313 RIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEVSPN 372
           R++E+I  QAAVHRC A ++        +PPT+N++ +Y+  ++V  +++G+E    +  
Sbjct: 307 RVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAP 366

Query: 373 FMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALAYSY 432
            MGSEDF++F++ +PG F  LG  +E  G     HSP Y+I+E+VLP GAAIHA++A  Y
Sbjct: 367 VMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQY 426

Query: 433 ILNSSS 439
           +   +S
Sbjct: 427 LKEKAS 428

BLAST of Lsi04G005100 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 444.1 bits (1141), Expect = 1.3e-124
Identity = 228/393 (58.02%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 46  LDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVVAF 105
           L+LA  P   + M  IRRKIHENPEL +EEFETSK IR ELD + V YR+PVA TG++ +
Sbjct: 38  LELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFPVAITGIIGY 97

Query: 106 VGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQLRQ 165
           +G+G PPFVALRADMDALPI+E VEWEHKSK  GKMHAC HD HVAMLLGA KIL Q RQ
Sbjct: 98  IGTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQ 157

Query: 166 KLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLAGC 225
            LQGTVVLIFQPAEE   GAK M  EG L  VEAIFG+HL  + P G  AS  G F+AG 
Sbjct: 158 HLQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGA 217

Query: 226 GSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGAA 285
           G+F+A I GKGGHAAIPQH+IDP++AAS+ ++SLQ +VSRE DP DS+VV+V  V  G A
Sbjct: 218 GAFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNA 277

Query: 286 LNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPPTI 345
            NVIPDS TI GT RAF+   F  L++RI+EII  QAAVHRC A ++     +  +PPT+
Sbjct: 278 FNVIPDSITIGGTLRAFT--GFTQLQERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTV 337

Query: 346 NDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHP 405
           N+  +Y+  ++V  +++G+E    +   MGSEDF++F++ +PG F  LG  +E  G    
Sbjct: 338 NNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YAS 397

Query: 406 PHSPHYKIDENVLPIGAAIHAALAYSYILNSSS 439
            HSPHY+I+E+VLP GAAIHA +A  Y+ + +S
Sbjct: 398 SHSPHYRINEDVLPYGAAIHATMAVQYLKDKAS 427

BLAST of Lsi04G005100 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 220/408 (53.92%), Postives = 291/408 (71.32%), Query Frame = 0

Query: 32  SLQTNHNSSLRHQILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHV 91
           S  +N  S +    L LA      +WM  IRR+IHENPEL +EE ETSKL++ ELD + V
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 92  SYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVA 151
           SY+ PVA TGV+ +VG+G  PFVALRADMDALPI+E+VEWEHKSK+ GKMHAC HDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 152 MLLGATKILNQLRQKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPV 211
           MLLGA K+L + +++LQGTV+L+FQPAEE G GAK ++  GVL+ V AIFGLH+ +   +
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 212 GIVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLD 271
           G ++SR G  +AG G FKA I GKGGHAA+PQ +IDP+LAAS  I+SLQ +VSRE DPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 272 SQVVSVAMVQAGAALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEI 331
           SQVV+VA  +   A NVIPDS TI GTFRA   KSF  L+ RI ++I  QA+V+ C A +
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 332 DFLGKEHPTIPPTINDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFL 391
           DFL  E P  PPT+N++ ++   + VS++++G E    +   M SEDFAF+   +PG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 392 FLGTYNERIGAIHPPHSPHYKIDENVLPIGAAIHAALAYSYILNSSST 440
           F+G  N+    +  PHSP ++++E +LP GA++ A+LA  Y+L+SSS+
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSSS 429

BLAST of Lsi04G005100 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.6e-122
Identity = 212/395 (53.67%), Postives = 281/395 (71.14%), Query Frame = 0

Query: 44  QILDLANHPTTVNWMKTIRRKIHENPELAFEEFETSKLIRQELDNLHVSYRWPVAGTGVV 103
           +IL L   P  V W+K +RR IHENPELAFEE+ETS+LIR ELD + + YR+P+A TG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 104 AFVGSGSPPFVALRADMDALPIEELVEWEHKSKVVGKMHACSHDAHVAMLLGATKILNQL 163
           A++GSG PPFVA+RADMDALPI+E VEWEH SKV GKMHAC HDAHV MLLGA  IL   
Sbjct: 135 AWIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAR 194

Query: 164 RQKLQGTVVLIFQPAEERGGGAKDMINEGVLDGVEAIFGLHLVHKYPVGIVASRPGEFLA 223
              L+GTVVL+FQPAEE G GAK+MI +G LD VEAIF +H+ H +P G++ SR G  LA
Sbjct: 195 EHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLA 254

Query: 224 GCGSFKAKIKGKGGHAAIPQHSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAG 283
           GCG F+A I  +    A      + +LAAS+A+ISLQ IVSRE  PLDSQVVSV     G
Sbjct: 255 GCGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGG 314

Query: 284 AALNVIPDSATIVGTFRAFSKKSFNALRDRIEEIINGQAAVHRCTAEIDFLGKEHPTIPP 343
            +L+V PD+  + GTFRAFS  SF  L+ RI+E++  Q  V  C A ++F  K++   PP
Sbjct: 315 HSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPP 374

Query: 344 TINDEQIYEHVRRVSMEIVGKEKTEVSPNFMGSEDFAFFSDKVPGSFLFLGTYNERIGAI 403
           T N++  Y H+++V+++++G     ++P  MG+EDFAF+S+ +P +F F+G  NE +G++
Sbjct: 375 TTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSV 434

Query: 404 HPPHSPHYKIDENVLPIGAAIHAALAYSYILNSSS 439
           H  HSPH+ IDE+ LP+GAA+HAA+A  Y+ +  S
Sbjct: 435 HIAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKHS 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84XG91.6e-13059.08IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S41.6e-13059.08IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
O043732.2e-12954.76IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
P549701.3e-12454.46IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Q5N8F21.1e-12357.40IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7ST022.3e-23091.52IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CVM42.0e-22991.29IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KQC35.8e-22990.40M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 P... [more]
A0A1S3BE495.4e-22790.63LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=365... [more]
A0A6J1FSW33.6e-21586.17IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
Match NameE-valueIdentityDescription
XP_038893400.16.5e-23593.50IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida][more]
KAA0034344.14.8e-23091.52IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa][more]
TYK15575.14.1e-22991.29IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa][more]
XP_004135471.31.2e-22890.40IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 ... [more]
XP_031741348.13.8e-22790.00IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G51760.11.6e-13054.76peptidase M20/M25/M40 family protein [more]
AT5G56660.19.1e-12654.46IAA-leucine resistant (ILR)-like 2 [more]
AT5G56650.11.3e-12458.02IAA-leucine resistant (ILR)-like 1 [more]
AT1G51780.11.2e-12253.92IAA-leucine resistant (ILR)-like gene 5 [more]
AT1G44350.11.6e-12253.67IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 224..323
e-value: 3.2E-11
score: 43.1
NoneNo IPR availableGENE3D3.30.70.360coord: 223..341
e-value: 1.5E-140
score: 470.1
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 60..418
e-value: 1.5E-140
score: 470.1
NoneNo IPR availablePANTHERPTHR11014:SF147IAA-AMINO ACID HYDROLASE ILR1-LIKE 2coord: 6..441
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 54..435
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 59..421
e-value: 6.0E-119
score: 395.4
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 45..436
e-value: 1.5E-127
score: 423.7
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 6..441
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 115..429
e-value: 1.9E-33
score: 116.2
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 58..433
e-value: 0.0
score: 644.758
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 223..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G005100.1Lsi04G005100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity