Lsi04G004250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G004250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Transmembrane amino acid transporter family protein
Location	chr04: 4068219 .. 4070530 (-)
RNA-Seq Expression	Lsi04G004250
Synteny	Lsi04G004250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTGTCTTATTTATTTAGATTTCTAGTGACTCTTTAGATCTAGTAGCAATTAGGCCAACTTGTAGTGATAAAATGGAGAGCCAAAACCAGGTTCCATCTACACAGGTTTTGGTTAAAGGCACCACCTTCTTAAGAACTTGTATCAATGGAATCAATGCATTATCAGGTTTCATATATATTTTTCTCCCTAAATCTCTATCTATATTATTAAATGGATGAACTTTGCTTTTTTTTTTTTTTTTTTTTTTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTAAAAAAAAAATATATTGTTACTAATTTTGAACATTTATAGGTGTGTGGGAATATTATCAATTCCTTTTGCCCTTTCTCAAGGAGGGTGGCTTAGCTTGATTCTTCTATTATTGGTAGCAACTATTTGTTGGTATACAGGTTTACTCTAATTAAAACATTGTATAGATTCAAATCCATTAATTGTAAAGACTTATCCTGATATATTGGTGGAGTAGCTTTTGGGTTCAAAGGAAAAATATTGGTTTCAAATTTTGTTTACTTGGAATTATATTTGGTGGCTGTTGAGTTCTTGATATTAGAAGGTGATATAATCTAGAGAAGCTTTTTCCAAGCCCAAATGGTTTCAAAATTGGGAGTATAAAAGTTGAAGGAAAGAAGATGTATATGATATTGACTTCTCTTTTAATATTGCCAACCACATGGATGAAGAGTTTGGGATGTCTAGCTAGCGTATGTTTCTTTTGGTGGTGTTTTGGCTTCTGTTGTTTTGATTTTGTGTGTTGCTTGGGTTGCTGCAATTGATGGTGTTGGGTTTAACCAAAGAGATGATCTTTTCAAACTACAAGGATTGCCCACTACTATAAGCTTGTTTGTCTTTTGTTATTGTGGACATTCTGTTTTTCCTATGCTTTGCAATTCCATGAAGGATAAAACCCAAGGTAAACTTCTTGATCATAAGCTTACTTATAATTAAGCTCAATTATAGTATCATATTAGGCCATGAGAAACTCAATTTTTACTATGTGTCTATCCACTTTTTTCCATTAAACTTTGAATTTTGATGAATTTTATCTTTTCAACTTTCAAATCTAGTAAGATTTAGAGTCCAAGTGGTGTCATTTTTACCTTTTATTTGACTAAATTCACCAGGGTCAATAACAAGAAGCTATAAAAAAAAATAACTTTATAAATTCATTAATTTCAACAAAAATAAAAATAGCACACAGAATGATTACTTAAAATCAATTGTGGAATAGATCTCTTAAAACTCATAAATTTTAACAAATATCAAACAAATTTAGTATTGGTTCTTATATTTTTTAGTATAAGTGAAGGTAGAGGTATTCGAACCACTGGTCACTAACACATACACATATGTCAGTTGAGTTATGTTCACTTTGGCTAGTATTGTTTTTCATTGAAGTACGTCCCTTAAAATTTGTAGTAAACATATACATCGTAATTCATTGCAAAACACAAAAAAAGCCCACATAAAAGAAAAATACTTTTTTAAAAAAAAATAATTCCTCAAATCAAAGTCTATGAAAAGTTTAAAAAAAAAAAATTGAATATATGCTTATGTAAATCTTTATTCAATCAAGTTTATAGTAAATTTATTTAAGAAAATGAGTATAATTCAACTAGCCTGAAACACGTACTATAAACTTCGAGGTTAAATGTTCCCTCTCATCTCACATATTAAAAAAATAATAATAATAAAGTATCTTTTTTCATTTTTATTTTAATTTATAAATTTGGAGAATCATACAAATAAATAAAAAATTGTTATAATATAGTTTTGCATTATTTGCAGGTTCTAGTAGTTTGCTTTGTGGGAAGCACGGTGAGTTATAGTTCGATGGCTATTTTAGGTTACCGAATGTATGGGGAAAACATAAAATCACAAGTAATATTAAACCTTCCAATTCAGAAAATGAGCACAAAAATAGCGATTTACACAACCCTTATCAATCCCATTACCAAATATGCAGCCATAACAAACCCAATTGCTATTGCTATTGAAGATTCAACTCCTTTATTATATTCACCACTCCAAAAATGGCCATTCTCATGA

mRNA sequence

ATGAGTGACTCTTTAGATCTAGTAGCAATTAGGCCAACTTGTAGTGATAAAATGGAGAGCCAAAACCAGGTTCCATCTACACAGGTTTTGGTTAAAGGCACCACCTTCTTAAGAACTTGTATCAATGGAATCAATGCATTATCAGAGAAGCTTTTTCCAAGCCCAAATGGTTTCAAAATTGGGAGTATAAAAGTTGAAGGAAAGAAGATGTATATGATATTGACTTCTCTTTTAATATTGCCAACCACATGGATGAAGACGTATGTTTCTTTTGGTGGTGTTTTGGCTTCTGTTGTTTTGATTTTGTGTGTTGCTTGGGTTGCTGCAATTGATGGTGTTGGGTTTAACCAAAGAGATGATCTTTTCAAACTACAAGGATTGCCCACTACTATAAGCTTGTTTGTCTTTTGTTATTGTGGACATTCTGTTTTTCCTATGCTTTGCAATTCCATGAAGGATAAAACCCAAGGTTCTAGTAGTTTGCTTTGTGGGAAGCACGGTTACCGAATGTATGGGGAAAACATAAAATCACAAGTAATATTAAACCTTCCAATTCAGAAAATGAGCACAAAAATAGCGATTTACACAACCCTTATCAATCCCATTACCAAATATGCAGCCATAACAAACCCAATTGCTATTGCTATTGAAGATTCAACTCCTTTATTATATTCACCACTCCAAAAATGGCCATTCTCATGA

Coding sequence (CDS)

ATGAGTGACTCTTTAGATCTAGTAGCAATTAGGCCAACTTGTAGTGATAAAATGGAGAGCCAAAACCAGGTTCCATCTACACAGGTTTTGGTTAAAGGCACCACCTTCTTAAGAACTTGTATCAATGGAATCAATGCATTATCAGAGAAGCTTTTTCCAAGCCCAAATGGTTTCAAAATTGGGAGTATAAAAGTTGAAGGAAAGAAGATGTATATGATATTGACTTCTCTTTTAATATTGCCAACCACATGGATGAAGACGTATGTTTCTTTTGGTGGTGTTTTGGCTTCTGTTGTTTTGATTTTGTGTGTTGCTTGGGTTGCTGCAATTGATGGTGTTGGGTTTAACCAAAGAGATGATCTTTTCAAACTACAAGGATTGCCCACTACTATAAGCTTGTTTGTCTTTTGTTATTGTGGACATTCTGTTTTTCCTATGCTTTGCAATTCCATGAAGGATAAAACCCAAGGTTCTAGTAGTTTGCTTTGTGGGAAGCACGGTTACCGAATGTATGGGGAAAACATAAAATCACAAGTAATATTAAACCTTCCAATTCAGAAAATGAGCACAAAAATAGCGATTTACACAACCCTTATCAATCCCATTACCAAATATGCAGCCATAACAAACCCAATTGCTATTGCTATTGAAGATTCAACTCCTTTATTATATTCACCACTCCAAAAATGGCCATTCTCATGA

Protein sequence

MSDSLDLVAIRPTCSDKMESQNQVPSTQVLVKGTTFLRTCINGINALSEKLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKTYVSFGGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLLCGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNPIAIAIEDSTPLLYSPLQKWPFS

Homology

[Close]

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot
Match: F4J1Q9 (Amino acid transporter AVT1I OS=Arabidopsis thaliana OX=3702 GN=AVT1I PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.0e-33
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0


Query: 51  LFPSPNGFKIGSI--KVEGKKMYMILTSLLILPTTW-----MKTYVSFGGVLASVVLILC 110
           LFP   GF I  I  ++ GK+ +M   + +I+PT W     + +YVS  GVLA+ V +  
Sbjct: 121 LFP---GFTIEMIGLRLNGKQAFMATVAFVIMPTLWWDNLSVLSYVSMSGVLATTVTLGS 180

Query: 111 VAWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL- 170
           ++W+ A DG+GF+Q+  L    G+PT +SL+ FCY  H V P L +SMK K Q ++ LL 
Sbjct: 181 ISWIGAFDGIGFHQKGKLINWSGIPTALSLYAFCYGAHPVLPTLYSSMKSKHQFNNVLLI 240

Query: 171 -----------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNP 226
                          GY MYG    SQ+ LNLPI K S+K+AIYTTL+NP+ KYA +  P
Sbjct: 241 CFILCTIGYTSMAVLGYLMYGSQTLSQITLNLPIHKTSSKVAIYTTLVNPVAKYALMITP 300

[Close]

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot
Match: Q8GYS4 (Amino acid transporter AVT1D OS=Arabidopsis thaliana OX=3702 GN=AVT1D PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-32
Identity = 78/202 (38.61%), Postives = 121/202 (59.90%), Query Frame = 0


Query: 40  CINGINALSEK---LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSF 99
           C+  I  +S+    LFP+ +      I ++  +++ ILT+LL+LPT W+K     +Y+S 
Sbjct: 250 CVEYIIMMSDNLSGLFPNVSLSIASGISLDSPQIFAILTTLLVLPTVWLKDLSLLSYLSV 309

Query: 100 GGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSM 159
           GGVLAS++L +C+ WV A+DG+GF+    +F L  LP TI +F F Y GHSVFP + +SM
Sbjct: 310 GGVLASILLGICLFWVGAVDGIGFHATGRVFDLSNLPVTIGIFGFGYSGHSVFPNIYSSM 369

Query: 160 KDKTQGSSSL-LCGKH-----------GYRMYGENIKSQVILNLPIQKMSTKIAIYTTLI 219
           KD ++    L +C              GY M+GE ++SQ  LN+P     +K+A++T +I
Sbjct: 370 KDPSRFPLVLVICFSFCTVLYIAVAVCGYTMFGEAVESQFTLNMPKHFFPSKVAVWTAVI 429

Query: 220 NPITKYAAITNPIAIAIEDSTP 222
            P+TKYA    PI +++E+  P
Sbjct: 430 TPMTKYALTITPIVMSLEELIP 451

[Close]

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot
Match: Q9LXF8 (Amino acid transporter AVT1J OS=Arabidopsis thaliana OX=3702 GN=AVT1J PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.7e-32
Identity = 77/185 (41.62%), Postives = 116/185 (62.70%), Query Frame = 0


Query: 50  KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCV 109
           KLF S  G     ++ +GK+M++I+ +L+ILP+ W+      +YVS  GV AS V++  +
Sbjct: 137 KLF-SNVGLNFMGLEFQGKQMFIIMVALIILPSVWLDNMRILSYVSASGVFASGVILASI 196

Query: 110 AWVAAIDGVGFNQRD-DLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL- 169
             V A +GVGF   D ++F+L G+ T++SL+ FCYC H VFP L  SMK+K Q S+ ++ 
Sbjct: 197 FSVGAFEGVGFKNNDSEVFRLNGVATSVSLYAFCYCAHPVFPTLYTSMKNKRQFSNVMII 256

Query: 170 -----------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNP 217
                          GY MYG +++SQ+ LNLP  K+S+K+AI+TTL+NPI K+A +  P
Sbjct: 257 CFTICTFIYASVAVLGYLMYGSDVESQITLNLPTDKLSSKVAIWTTLVNPIAKFALMVTP 316

[Close]

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot
Match: F4JE35 (Amino acid transporter AVT1B OS=Arabidopsis thaliana OX=3702 GN=AVT1B PE=3 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 8.6e-32
Identity = 70/191 (36.65%), Postives = 116/191 (60.73%), Query Frame = 0


Query: 51  LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCVA 110
           +FP+ +   IG   ++  +++ +LT+L +LPT W++     +Y+S GGV+ASV+++LC+ 
Sbjct: 255 MFPNAS-LSIGGFHLDAPRLFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLF 314

Query: 111 WVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLLC-- 170
           WV  +D VG + +     L  LP ++ L+ +CY GH VFP +  SM   +Q S+ LL   
Sbjct: 315 WVGLVDDVGIHSKGTPLNLATLPVSVGLYGYCYSGHGVFPNIYTSMAKPSQFSAVLLASF 374

Query: 171 ----------GKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNPIA 225
                        GY M+GE+ +SQ  LNLP   +++KIA++TT++NP TKYA   +P+A
Sbjct: 375 GICTLMYAGVAVMGYSMFGESTESQFTLNLPQDLVASKIALWTTVVNPFTKYALTLSPVA 434

[Close]

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot
Match: F4IUW3 (Amino acid transporter AVT1C OS=Arabidopsis thaliana OX=3702 GN=AVT1C PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.6e-32
Identity = 69/188 (36.70%), Postives = 114/188 (60.64%), Query Frame = 0


Query: 51  LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCVA 110
           L+P+     IG  +++ + ++ +LT+L +LPT W++     +Y+S GGV+ASV+++LC+ 
Sbjct: 265 LYPNA-ALSIGGFQLDARHLFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLF 324

Query: 111 WVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL--- 170
           W+  +D VG + +     L  LP  I L+ +CY GH+VFP +  SM   +Q  + LL   
Sbjct: 325 WIGLVDEVGIHSKGTTLNLSTLPVAIGLYGYCYSGHAVFPNIYTSMAKPSQYPAVLLTCF 384

Query: 171 ---------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNPIA 222
                        GY M+GE+ +SQ  LNLP   ++TKIA++TT++NP TKYA   +P+A
Sbjct: 385 GICTLMYAGVAVMGYTMFGESTQSQFTLNLPQDLIATKIAVWTTVVNPFTKYALTISPVA 444

[Close]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL
Match: A0A6J1J733 (amino acid transporter AVT1I-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484061 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 8.3e-70
Identity = 156/289 (53.98%), Postives = 183/289 (63.32%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PS Q  +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSPQFQLKGTTFLRTCINGINALSVVCWYTGLLLKRCMDANPSVRTYSDIGWL 60

Query: 78  ---------------------------------EKLFPSPNGFKIGSIKVEGKKMYMILT 137
                                            +KLFPS +GFK+GS+ VEGK+MYM+L 
Sbjct: 61  AFGFKGKIMVSIFVYMELYLVAVEFLILEGDNLQKLFPS-SGFKVGSVSVEGKQMYMVLA 120

Query: 138 SLLILPTTWMKT-----YVSFGGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTT 197
           ++ ILPTTW+K+     YVSFGGVLAS VL+LCVAWV A+DGVGFNQRD+L KLQGLPTT
Sbjct: 121 AVFILPTTWLKSLGLLAYVSFGGVLASAVLVLCVAWVGAVDGVGFNQRDELLKLQGLPTT 180

Query: 198 ISLFVFCYCGHSVFPMLCNSMKDKTQ----------------GSSSLLCGKHGYRMYGEN 224
           +SLF+FCYCGH+VFPMLCNSM +KTQ                GS ++L    GY MYGEN
Sbjct: 181 LSLFLFCYCGHAVFPMLCNSMNNKTQFPKVLMVCFVASTMSYGSMAIL----GYLMYGEN 240

[Close]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL
Match: A0A6J1H1R5 (amino acid transporter AVT1J-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458792 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 7.0e-69
Identity = 154/288 (53.47%), Postives = 184/288 (63.89%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PSTQ+ +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSTQLQLKGTTFLRTCINGINALSAVCWYTGLLLKRCMDANPSVRTYPDIGWL 60

Query: 78  ---------------------------------EKLFPSPNGFKIGSIKVEGKKMYMILT 137
                                            +KLFPS +GFK+GS+ VEGK+MYM+L 
Sbjct: 61  AFGFKGKIMVSVFVYMELYLVAVEFLILEGDNLQKLFPS-SGFKVGSVSVEGKQMYMVLA 120

Query: 138 SLLILPTTWMKT-----YVSFGGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTT 197
           ++ ILPTTW+K+     YVSFGGVLASVVL+LCVAWV A+DGVGFNQR++L KLQGLPTT
Sbjct: 121 AVFILPTTWLKSLGLLAYVSFGGVLASVVLVLCVAWVGAVDGVGFNQRNELLKLQGLPTT 180

Query: 198 ISLFVFCYCGHSVFPMLCNSMKDKTQ----------------GSSSLLCGKHGYRMYGEN 223
           +SLF+FCYCGH+VFPML NSM +KTQ                GS ++L    GY MYG+N
Sbjct: 181 LSLFLFCYCGHAVFPMLSNSMNNKTQFPKVLMVCFVTSTMSYGSMAIL----GYLMYGDN 240

[Close]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL
Match: A0A6J1JG00 (amino acid transporter AVT1I-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484061 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.6e-67
Identity = 156/315 (49.52%), Postives = 183/315 (58.10%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PS Q  +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSPQFQLKGTTFLRTCINGINALSGVGTLSIPFALSQGGWLSLILLSLVAVVC 60

Query: 78  -----------------------------------------------------------E 137
                                                                      +
Sbjct: 61  WYTGLLLKRCMDANPSVRTYSDIGWLAFGFKGKIMVSIFVYMELYLVAVEFLILEGDNLQ 120

Query: 138 KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKT-----YVSFGGVLASVVLILCV 197
           KLFPS +GFK+GS+ VEGK+MYM+L ++ ILPTTW+K+     YVSFGGVLAS VL+LCV
Sbjct: 121 KLFPS-SGFKVGSVSVEGKQMYMVLAAVFILPTTWLKSLGLLAYVSFGGVLASAVLVLCV 180

Query: 198 AWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQ-------- 224
           AWV A+DGVGFNQRD+L KLQGLPTT+SLF+FCYCGH+VFPMLCNSM +KTQ        
Sbjct: 181 AWVGAVDGVGFNQRDELLKLQGLPTTLSLFLFCYCGHAVFPMLCNSMNNKTQFPKVLMVC 240

[Close]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL
Match: A0A6J1H012 (amino acid transporter AVT1J-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458792 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 7.3e-66
Identity = 154/314 (49.04%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PSTQ+ +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSTQLQLKGTTFLRTCINGINALSGVGILSIPFALSQGGWLSLILLSLVAAVC 60

Query: 78  -----------------------------------------------------------E 137
                                                                      +
Sbjct: 61  WYTGLLLKRCMDANPSVRTYPDIGWLAFGFKGKIMVSVFVYMELYLVAVEFLILEGDNLQ 120

Query: 138 KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKT-----YVSFGGVLASVVLILCV 197
           KLFPS +GFK+GS+ VEGK+MYM+L ++ ILPTTW+K+     YVSFGGVLASVVL+LCV
Sbjct: 121 KLFPS-SGFKVGSVSVEGKQMYMVLAAVFILPTTWLKSLGLLAYVSFGGVLASVVLVLCV 180

Query: 198 AWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQ-------- 223
           AWV A+DGVGFNQR++L KLQGLPTT+SLF+FCYCGH+VFPML NSM +KTQ        
Sbjct: 181 AWVGAVDGVGFNQRNELLKLQGLPTTLSLFLFCYCGHAVFPMLSNSMNNKTQFPKVLMVC 240

[Close]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL
Match: A0A0A0KSP8 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605130 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.0e-64
Identity = 162/321 (50.47%), Postives = 180/321 (56.07%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           MESQNQ+PSTQ L KGTTFLRTCINGINALS                             
Sbjct: 1   MESQNQLPSTQFLAKGTTFLRTCINGINALSGVGILSIPFAISQGGWVSLILLLMVAIIC 60

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 61  CYTASLLKHCMDANPLMVRTYTDIGGLAFGYKGRILVSVFVYLELYLVAVEFLILEGDNL 120

Query: 138 EKLFPSPN---GFKIGSIKV-EG----KKMYMILTSLLILPTTWMK-----TYVSFGGVL 197
           EKLFPS +   G KIGS+KV EG    KKMYMIL+++LILPTTW+K      YVSFGGVL
Sbjct: 121 EKLFPSSSPLFGLKIGSLKVDEGMMNYKKMYMILSAVLILPTTWVKNLGSLAYVSFGGVL 180

Query: 198 ASVVLILCVAWVAAIDGVGFNQRDD-----LFKLQGLPTTISLFVFCYCGHSVFPMLCNS 220
           AS+VL+LCV W+ A DG GFNQR D     +  L GLPTTISLFVFCYCGHSVFPMLCNS
Sbjct: 181 ASIVLVLCVGWIGATDGFGFNQRSDNDHHRVLNLHGLPTTISLFVFCYCGHSVFPMLCNS 240

[Close]

BLAST of Lsi04G004250 vs. NCBI nr
Match: XP_022986262.1 (amino acid transporter AVT1I-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 273.5 bits (698), Expect = 1.7e-69
Identity = 156/289 (53.98%), Postives = 183/289 (63.32%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PS Q  +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSPQFQLKGTTFLRTCINGINALSVVCWYTGLLLKRCMDANPSVRTYSDIGWL 60

Query: 78  ---------------------------------EKLFPSPNGFKIGSIKVEGKKMYMILT 137
                                            +KLFPS +GFK+GS+ VEGK+MYM+L 
Sbjct: 61  AFGFKGKIMVSIFVYMELYLVAVEFLILEGDNLQKLFPS-SGFKVGSVSVEGKQMYMVLA 120

Query: 138 SLLILPTTWMKT-----YVSFGGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTT 197
           ++ ILPTTW+K+     YVSFGGVLAS VL+LCVAWV A+DGVGFNQRD+L KLQGLPTT
Sbjct: 121 AVFILPTTWLKSLGLLAYVSFGGVLASAVLVLCVAWVGAVDGVGFNQRDELLKLQGLPTT 180

Query: 198 ISLFVFCYCGHSVFPMLCNSMKDKTQ----------------GSSSLLCGKHGYRMYGEN 224
           +SLF+FCYCGH+VFPMLCNSM +KTQ                GS ++L    GY MYGEN
Sbjct: 181 LSLFLFCYCGHAVFPMLCNSMNNKTQFPKVLMVCFVASTMSYGSMAIL----GYLMYGEN 240

[Close]

BLAST of Lsi04G004250 vs. NCBI nr
Match: XP_022957374.1 (amino acid transporter AVT1J-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 270.4 bits (690), Expect = 1.5e-68
Identity = 154/288 (53.47%), Postives = 184/288 (63.89%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PSTQ+ +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSTQLQLKGTTFLRTCINGINALSAVCWYTGLLLKRCMDANPSVRTYPDIGWL 60

Query: 78  ---------------------------------EKLFPSPNGFKIGSIKVEGKKMYMILT 137
                                            +KLFPS +GFK+GS+ VEGK+MYM+L 
Sbjct: 61  AFGFKGKIMVSVFVYMELYLVAVEFLILEGDNLQKLFPS-SGFKVGSVSVEGKQMYMVLA 120

Query: 138 SLLILPTTWMKT-----YVSFGGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTT 197
           ++ ILPTTW+K+     YVSFGGVLASVVL+LCVAWV A+DGVGFNQR++L KLQGLPTT
Sbjct: 121 AVFILPTTWLKSLGLLAYVSFGGVLASVVLVLCVAWVGAVDGVGFNQRNELLKLQGLPTT 180

Query: 198 ISLFVFCYCGHSVFPMLCNSMKDKTQ----------------GSSSLLCGKHGYRMYGEN 223
           +SLF+FCYCGH+VFPML NSM +KTQ                GS ++L    GY MYG+N
Sbjct: 181 LSLFLFCYCGHAVFPMLSNSMNNKTQFPKVLMVCFVTSTMSYGSMAIL----GYLMYGDN 240

[Close]

BLAST of Lsi04G004250 vs. NCBI nr
Match: XP_022986253.1 (amino acid transporter AVT1I-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 263.5 bits (672), Expect = 1.8e-66
Identity = 156/315 (49.52%), Postives = 183/315 (58.10%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PS Q  +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSPQFQLKGTTFLRTCINGINALSGVGTLSIPFALSQGGWLSLILLSLVAVVC 60

Query: 78  -----------------------------------------------------------E 137
                                                                      +
Sbjct: 61  WYTGLLLKRCMDANPSVRTYSDIGWLAFGFKGKIMVSIFVYMELYLVAVEFLILEGDNLQ 120

Query: 138 KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKT-----YVSFGGVLASVVLILCV 197
           KLFPS +GFK+GS+ VEGK+MYM+L ++ ILPTTW+K+     YVSFGGVLAS VL+LCV
Sbjct: 121 KLFPS-SGFKVGSVSVEGKQMYMVLAAVFILPTTWLKSLGLLAYVSFGGVLASAVLVLCV 180

Query: 198 AWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQ-------- 224
           AWV A+DGVGFNQRD+L KLQGLPTT+SLF+FCYCGH+VFPMLCNSM +KTQ        
Sbjct: 181 AWVGAVDGVGFNQRDELLKLQGLPTTLSLFLFCYCGHAVFPMLCNSMNNKTQFPKVLMVC 240

[Close]

BLAST of Lsi04G004250 vs. NCBI nr
Match: KAG6601432.1 (Amino acid transporter AVT1J, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 262.3 bits (669), Expect = 4.0e-66
Identity = 155/314 (49.36%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PSTQ+ +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSTQLQLKGTTFLRTCINGINALSGVGILSIPFALSQGGWLSLILLSLVAAVC 60

Query: 78  -----------------------------------------------------------E 137
                                                                      +
Sbjct: 61  WYTGLLLKRCMDANPSVRTYPDIGWLAFGFKGKIMVSIFVYMELYLVAVEFLILEGDNLQ 120

Query: 138 KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKT-----YVSFGGVLASVVLILCV 197
           KLFPS +GFK+GS+ VEGK+MYM+L ++ ILPTTW+K+     YVSFGGVLASVVL+LCV
Sbjct: 121 KLFPS-SGFKVGSVSVEGKQMYMVLAAVFILPTTWLKSLGLLAYVSFGGVLASVVLVLCV 180

Query: 198 AWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQ-------- 223
           AWV A+DGVGFNQRD+L KLQGLPTT+SLF+FCYCGH+VFPML NSM +KTQ        
Sbjct: 181 AWVGAVDGVGFNQRDELLKLQGLPTTLSLFLFCYCGHAVFPMLSNSMNNKTQFPKVLMVC 240

[Close]

BLAST of Lsi04G004250 vs. NCBI nr
Match: XP_023512552.1 (amino acid transporter AVT1I-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.9 bits (668), Expect = 5.2e-66
Identity = 157/314 (50.00%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 18  MESQNQVPSTQVLVKGTTFLRTCINGINALS----------------------------- 77
           ME+Q+Q+PSTQ+ +KGTTFLRTCINGINALS                             
Sbjct: 1   METQSQLPSTQLQLKGTTFLRTCINGINALSGVGILSIPFALSQGGWLSLILLSLVAAVC 60

Query: 78  -----------------------------------------------------------E 137
                                                                      +
Sbjct: 61  WYTGLLLKRCMDANPSVRTYPDIGWLAFGFKGKIMVSVFVYMELYLVAVEFLILEGDNLQ 120

Query: 138 KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMKT-----YVSFGGVLASVVLILCV 197
           KLFPS +GFK GS+ VEGK+MYM+L ++LILPTT +K+     YVSFGGVLASVVL+LCV
Sbjct: 121 KLFPS-SGFKFGSVSVEGKQMYMVLAAVLILPTTCLKSLGLLAYVSFGGVLASVVLVLCV 180

Query: 198 AWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQ-------- 223
           AWV A+DGVGFNQRD+L KLQGLPTT+SLF+FCYCGH+VFPMLCNSM +KTQ        
Sbjct: 181 AWVGAVDGVGFNQRDELLKLQGLPTTLSLFLFCYCGHAVFPMLCNSMNNKTQFPKVLMVC 240

[Close]

BLAST of Lsi04G004250 vs. TAIR 10
Match: AT3G28960.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 142.5 bits (358), Expect = 4.3e-34
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0


Query: 51  LFPSPNGFKIGSI--KVEGKKMYMILTSLLILPTTW-----MKTYVSFGGVLASVVLILC 110
           LFP   GF I  I  ++ GK+ +M   + +I+PT W     + +YVS  GVLA+ V +  
Sbjct: 121 LFP---GFTIEMIGLRLNGKQAFMATVAFVIMPTLWWDNLSVLSYVSMSGVLATTVTLGS 180

Query: 111 VAWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL- 170
           ++W+ A DG+GF+Q+  L    G+PT +SL+ FCY  H V P L +SMK K Q ++ LL 
Sbjct: 181 ISWIGAFDGIGFHQKGKLINWSGIPTALSLYAFCYGAHPVLPTLYSSMKSKHQFNNVLLI 240

Query: 171 -----------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNP 226
                          GY MYG    SQ+ LNLPI K S+K+AIYTTL+NP+ KYA +  P
Sbjct: 241 CFILCTIGYTSMAVLGYLMYGSQTLSQITLNLPIHKTSSKVAIYTTLVNPVAKYALMITP 300

[Close]

BLAST of Lsi04G004250 vs. TAIR 10
Match: AT5G02180.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 141.7 bits (356), Expect = 7.3e-34
Identity = 78/202 (38.61%), Postives = 121/202 (59.90%), Query Frame = 0


Query: 40  CINGINALSEK---LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSF 99
           C+  I  +S+    LFP+ +      I ++  +++ ILT+LL+LPT W+K     +Y+S 
Sbjct: 250 CVEYIIMMSDNLSGLFPNVSLSIASGISLDSPQIFAILTTLLVLPTVWLKDLSLLSYLSV 309

Query: 100 GGVLASVVLILCVAWVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSM 159
           GGVLAS++L +C+ WV A+DG+GF+    +F L  LP TI +F F Y GHSVFP + +SM
Sbjct: 310 GGVLASILLGICLFWVGAVDGIGFHATGRVFDLSNLPVTIGIFGFGYSGHSVFPNIYSSM 369

Query: 160 KDKTQGSSSL-LCGKH-----------GYRMYGENIKSQVILNLPIQKMSTKIAIYTTLI 219
           KD ++    L +C              GY M+GE ++SQ  LN+P     +K+A++T +I
Sbjct: 370 KDPSRFPLVLVICFSFCTVLYIAVAVCGYTMFGEAVESQFTLNMPKHFFPSKVAVWTAVI 429

Query: 220 NPITKYAAITNPIAIAIEDSTP 222
            P+TKYA    PI +++E+  P
Sbjct: 430 TPMTKYALTITPIVMSLEELIP 451

[Close]

BLAST of Lsi04G004250 vs. TAIR 10
Match: AT5G15240.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-33
Identity = 77/185 (41.62%), Postives = 116/185 (62.70%), Query Frame = 0


Query: 50  KLFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCV 109
           KLF S  G     ++ +GK+M++I+ +L+ILP+ W+      +YVS  GV AS V++  +
Sbjct: 137 KLF-SNVGLNFMGLEFQGKQMFIIMVALIILPSVWLDNMRILSYVSASGVFASGVILASI 196

Query: 110 AWVAAIDGVGFNQRD-DLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL- 169
             V A +GVGF   D ++F+L G+ T++SL+ FCYC H VFP L  SMK+K Q S+ ++ 
Sbjct: 197 FSVGAFEGVGFKNNDSEVFRLNGVATSVSLYAFCYCAHPVFPTLYTSMKNKRQFSNVMII 256

Query: 170 -----------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNP 217
                          GY MYG +++SQ+ LNLP  K+S+K+AI+TTL+NPI K+A +  P
Sbjct: 257 CFTICTFIYASVAVLGYLMYGSDVESQITLNLPTDKLSSKVAIWTTLVNPIAKFALMVTP 316

[Close]

BLAST of Lsi04G004250 vs. TAIR 10
Match: AT2G39130.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 138.7 bits (348), Expect = 6.1e-33
Identity = 69/188 (36.70%), Postives = 114/188 (60.64%), Query Frame = 0


Query: 51  LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCVA 110
           L+P+     IG  +++ + ++ +LT+L +LPT W++     +Y+S GGV+ASV+++LC+ 
Sbjct: 265 LYPNA-ALSIGGFQLDARHLFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLF 324

Query: 111 WVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLL--- 170
           W+  +D VG + +     L  LP  I L+ +CY GH+VFP +  SM   +Q  + LL   
Sbjct: 325 WIGLVDEVGIHSKGTTLNLSTLPVAIGLYGYCYSGHAVFPNIYTSMAKPSQYPAVLLTCF 384

Query: 171 ---------CGKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITNPIA 222
                        GY M+GE+ +SQ  LNLP   ++TKIA++TT++NP TKYA   +P+A
Sbjct: 385 GICTLMYAGVAVMGYTMFGESTQSQFTLNLPQDLIATKIAVWTTVVNPFTKYALTISPVA 444

[Close]

BLAST of Lsi04G004250 vs. TAIR 10
Match: AT3G54830.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 65/177 (36.72%), Postives = 105/177 (59.32%), Query Frame = 0


Query: 51  LFPSPNGFKIGSIKVEGKKMYMILTSLLILPTTWMK-----TYVSFGGVLASVVLILCVA 110
           +FP+ +   IG   ++  +++ +LT+L +LPT W++     +Y+S GGV+ASV+++LC+ 
Sbjct: 255 MFPNAS-LSIGGFHLDAPRLFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLF 314

Query: 111 WVAAIDGVGFNQRDDLFKLQGLPTTISLFVFCYCGHSVFPMLCNSMKDKTQGSSSLLC-- 170
           WV  +D VG + +     L  LP ++ L+ +CY GH VFP +  SM   +Q S+ LL   
Sbjct: 315 WVGLVDDVGIHSKGTPLNLATLPVSVGLYGYCYSGHGVFPNIYTSMAKPSQFSAVLLASF 374

Query: 171 ----------GKHGYRMYGENIKSQVILNLPIQKMSTKIAIYTTLINPITKYAAITN 211
                        GY M+GE+ +SQ  LNLP   +++KIA++TT++NP TKY    N
Sbjct: 375 GICTLMYAGVAVMGYSMFGESTESQFTLNLPQDLVASKIALWTTVVNPFTKYPLSRN 430

The following BLAST results are available for this feature:

BLAST of Lsi04G004250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4J1Q9	6.0e-33	41.75	Amino acid transporter AVT1I OS=Arabidopsis thaliana OX=3702 GN=AVT1I PE=3 SV=1	[more]
Q8GYS4	1.0e-32	38.61	Amino acid transporter AVT1D OS=Arabidopsis thaliana OX=3702 GN=AVT1D PE=2 SV=1	[more]
Q9LXF8	1.7e-32	41.62	Amino acid transporter AVT1J OS=Arabidopsis thaliana OX=3702 GN=AVT1J PE=2 SV=1	[more]
F4JE35	8.6e-32	36.65	Amino acid transporter AVT1B OS=Arabidopsis thaliana OX=3702 GN=AVT1B PE=3 SV=2	[more]
F4IUW3	8.6e-32	36.70	Amino acid transporter AVT1C OS=Arabidopsis thaliana OX=3702 GN=AVT1C PE=1 SV=1	[more]

BLAST of Lsi04G004250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J733	8.3e-70	53.98	amino acid transporter AVT1I-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1H1R5	7.0e-69	53.47	amino acid transporter AVT1J-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1JG00	8.6e-67	49.52	amino acid transporter AVT1I-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1H012	7.3e-66	49.04	amino acid transporter AVT1J-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KSP8	1.0e-64	50.47	Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605130 PE...	[more]

BLAST of Lsi04G004250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986262.1	1.7e-69	53.98	amino acid transporter AVT1I-like isoform X2 [Cucurbita maxima]	[more]
XP_022957374.1	1.5e-68	53.47	amino acid transporter AVT1J-like isoform X2 [Cucurbita moschata]	[more]
XP_022986253.1	1.8e-66	49.52	amino acid transporter AVT1I-like isoform X1 [Cucurbita maxima]	[more]
KAG6601432.1	4.0e-66	49.36	Amino acid transporter AVT1J, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023512552.1	5.2e-66	50.00	amino acid transporter AVT1I-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi04G004250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28960.1	4.3e-34	41.75	Transmembrane amino acid transporter family protein	[more]
AT5G02180.1	7.3e-34	38.61	Transmembrane amino acid transporter family protein	[more]
AT5G15240.1	1.2e-33	41.62	Transmembrane amino acid transporter family protein	[more]
AT2G39130.1	6.1e-33	36.70	Transmembrane amino acid transporter family protein	[more]
AT3G54830.1	1.8e-29	36.72	Transmembrane amino acid transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013057	Amino acid transporter, transmembrane domain	PFAM	PF01490	Aa_trans	coord: 68..218 e-value: 1.9E-10 score: 40.0
None	No IPR available	PANTHER	PTHR48017	OS05G0424000 PROTEIN-RELATED	coord: 49..222
None	No IPR available	PANTHER	PTHR48017:SF109	OS06G0228500 PROTEIN	coord: 49..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G004250.1	Lsi04G004250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane