Lsi04G004230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G004230
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease T(2)
Location	chr04: 4053694 .. 4055514 (-)
RNA-Seq Expression	Lsi04G004230
Synteny	Lsi04G004230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAAGATGGGGATTTAAAGAATTAAGAAATCCATCACTTGACGCAACCAATAATATGTTGTCGTACAAGCACAAAAAAGCATATCTTAAGCAATTTTCTCGCTTATCCTTTTTGCCTATAAAAGTTAATAACTTTTCATCGAACTCAACAAAAAAACATGAAGCCCTACTACTCAATTTTCATCTTCAAGCTTCTCCTTTTGCAATGTCTATCATCCCTTTGTCTTTCTCAGGACTTCGATTTCTTCTACTTTGTTCAGCAGGTCAGTCAAATTATCTTTATTTATTTATTTATTTATTTTCTTTTGTGAATTAATCAAAAATTAAATTAATTACTGTGTATTTTGAGAGATTTGCAGTGGCCGGGAGCATATTGTGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGGATTCATGGTCTTTGGCCTAATTACAATGATGGAACCTACCCATCCAACTGCGATCCCGACAGTCTTTTCGACCGAACTGAGGTATTTTAAAAGCTTGTTTGGATTGACTTAAAAAAAAAAATGTAAAATTTTTTTTATAGCTATGGTTTATGTATAATAATATAATTTATTCATATTTAGATCTCAGAAGTATTAAGCAGCATGCAAAAACACTGGCCATCGTTAAGCTGTCCAAGCAGCAATGGGCTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAATCTGAACTCGACCAGAAAGAATATTTTGAGGCAGCTATTAAACTCAAGGAGAAGGCCAATCTCCTTAAACTCCTTAATAATGCAGGTACCACGTAATCGTAAACCGTTAAAAGTATTGATATGATTAAATTTACCGTAACTCATTAGTTAGAGCTTTTTAATTGATTAGTAGTGATTTAAATAGTATTTAGGAGTTTCTATGTCATTTCCTCCGAGTAATTTTGATTTCAAGCCCATGAGAGAAGTATAGTTTTTGGGTTGGCAAAGCAGGAAAATTCTCATAATGAATGACAAAATTATTAAATATGTTGACGCACGATTTTGGCGCAAGTTGACTTGATCATAGAGAATCTACGTGTACTTACAAAACAAAATCTCAATATCGGTGTGATGCTCATTCCAATAGACTCCAATATTTAAGTTAGTATTGAACAAATAGAACTGAGTGGAGGATATTATGTACCTCTTGTAGCTTGGGATATTTTCCTATTTATATCCTTAAAATTCTTGACAAATATGCTTGTGGGCTTTGTTTGTGCCGGTGGGGAGACCAATCATCTGTTCTAACTTACAACTGGAGGTCGTTTTTACCTCTTTTGGGTTTGAGTCATTCCTCGCCTCAACTATGAGTCTGGGAAATTGCCCCTTTTTCTTTTCGACACTTGGGTTTATCACATCTCGTGGGTCGTAGCGAACGAAATTTGCACCACTTATTCTAAATTTTTGTTCATATGAAAGGTCTAGGAATTTTAACACAACAATTTTGACTTTAAGCATGTGAATGAATTGCAGGAATTGAAGCAAATGACGAGTTTTACAGTTTGGAGAGTGTAAGAAATGCCATAGAAGAGGGGATTGGATATACTCCAGGGATTGAATGTAACAAGGATTCAGCTGGCAATGCTCAACTTTATCAAATTTATTTATGTGTTGATACTTCTGGTTCTCAGTTCATCAAATGCCCAATTCTTCCCAAGGGAAAATGTGCTTCCACTATCCAATTCCCTAAGTTTTAAATTAATCACTTTCATCTCATCTACCTACACCTACATTAATTTGATTTTTTTTTTTTTTTTTTTAATTCCCTTG

mRNA sequence

AGAGAAGATGGGGATTTAAAGAATTAAGAAATCCATCACTTGACGCAACCAATAATATGTTGTCGTACAAGCACAAAAAAGCATATCTTAAGCAATTTTCTCGCTTATCCTTTTTGCCTATAAAAGTTAATAACTTTTCATCGAACTCAACAAAAAAACATGAAGCCCTACTACTCAATTTTCATCTTCAAGCTTCTCCTTTTGCAATGTCTATCATCCCTTTGTCTTTCTCAGGACTTCGATTTCTTCTACTTTGTTCAGCAGTGGCCGGGAGCATATTGTGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGGATTCATGGTCTTTGGCCTAATTACAATGATGGAACCTACCCATCCAACTGCGATCCCGACAGTCTTTTCGACCGAACTGAGATCTCAGAAGTATTAAGCAGCATGCAAAAACACTGGCCATCGTTAAGCTGTCCAAGCAGCAATGGGCTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAATCTGAACTCGACCAGAAAGAATATTTTGAGGCAGCTATTAAACTCAAGGAGAAGGCCAATCTCCTTAAACTCCTTAATAATGCAGGTACCACGTAATCGTAAACCGTTAAAAGTATTGATATGATTAAATTTACCGTAACTCATTAGTTAGAGCTTTTTAATTGATTAGTAGTGATTTAAATAGTATTTAGGAGTTTCTATGTCATTTCCTCCGAGTAATTTTGATTTCAAGCCCATGAGAGAAGTATAGTTTTTGGGTTGGCAAAGCAGGAAAATTCTCATAATGAATGACAAAATTATTAAATATGTTGACGCACGATTTTGGCGCAAGTTGACTTGATCATAGAGAATCTACGTGTACTTACAAAACAAAATCTCAATATCGGTGTGATGCTCATTCCAATAGACTCCAATATTTAAGTTAGTATTGAACAAATAGAACTGAGTGGAGGATATTATGTACCTCTTGTAGCTTGGGATATTTTCCTATTTATATCCTTAAAATTCTTGACAAATATGCTTGTGGGCTTTGTTTGTGCCGGTGGGGAGACCAATCATCTGTTCTAACTTACAACTGGAGGTCGTTTTTACCTCTTTTGGGTTTGAGTCATTCCTCGCCTCAACTATGAGTCTGGGAAATTGCCCCTTTTTCTTTTCGACACTTGGGTTTATCACATCTCGTGGGTCGTAGCGAACGAAATTTGCACCACTTATTCTAAATTTTTGTTCATATGAAAGGTCTAGGAATTTTAACACAACAATTTTGACTTTAAGCATGTGAATGAATTGCAGGAATTGAAGCAAATGACGAGTTTTACAGTTTGGAGAGTGTAAGAAATGCCATAGAAGAGGGGATTGGATATACTCCAGGGATTGAATGTAACAAGGATTCAGCTGGCAATGCTCAACTTTATCAAATTTATTTATGTGTTGATACTTCTGGTTCTCAGTTCATCAAATGCCCAATTCTTCCCAAGGGAAAATGTGCTTCCACTATCCAATTCCCTAAGTTTTAAATTAATCACTTTCATCTCATCTACCTACACCTACATTAATTTGATTTTTTTTTTTTTTTTTTTAATTCCCTTG

Coding sequence (CDS)

ATGAAGCCCTACTACTCAATTTTCATCTTCAAGCTTCTCCTTTTGCAATGTCTATCATCCCTTTGTCTTTCTCAGGACTTCGATTTCTTCTACTTTGTTCAGCAGTGGCCGGGAGCATATTGTGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGGATTCATGGTCTTTGGCCTAATTACAATGATGGAACCTACCCATCCAACTGCGATCCCGACAGTCTTTTCGACCGAACTGAGATCTCAGAAGTATTAAGCAGCATGCAAAAACACTGGCCATCGTTAAGCTGTCCAAGCAGCAATGGGCTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAATCTGAACTCGACCAGAAAGAATATTTTGAGGCAGCTATTAAACTCAAGGAGAAGGCCAATCTCCTTAAACTCCTTAATAATGCAGGTACCACGTAA

Protein sequence

MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAGTT

Homology

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BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 2.6e-56
Identity = 97/151 (64.24%), Postives = 126/151 (83.44%), Query Frame = 0


Query: 5   YSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHG 64
           +S+F+  L++ QCLS L  ++DFDFFYFVQQWPG+YCDTKQ  CCYP TG+PAADFGIHG
Sbjct: 7   FSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQS-CCYPTTGKPAADFGIHG 66

Query: 65  LWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTC 124
           LWPN NDGTYPSNCDP+S +D+++IS+++SSMQ++WP+L+CPS +G  FWSHEWEKHGTC
Sbjct: 67  LWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTC 126

Query: 125 SESEL-DQKEYFEAAIKLKEKANLLKLLNNA 155
           +ES L +Q  YF+ A+ LK + +LL +L  A
Sbjct: 127 AESVLTNQHAYFKKALDLKNQIDLLSILQGA 156

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BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 2.6e-56
Identity = 97/148 (65.54%), Postives = 121/148 (81.76%), Query Frame = 0


Query: 8   FIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWP 67
           FIF +L LQ L     +QDFDFFYFV QWPGAYCD++   CCYP+TG+PAADFGIHGLWP
Sbjct: 4   FIF-ILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHS-CCYPQTGKPAADFGIHGLWP 63

Query: 68  NYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSES 127
           NY  G +P NC+PDS FD   +S+++S +Q+ WP+LSCPS++G++FW+HEWEKHGTC+ES
Sbjct: 64  NYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAES 123

Query: 128 ELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           ELDQ +YFEA +KLK+KANLL  L NAG
Sbjct: 124 ELDQHDYFEAGLKLKQKANLLHALTNAG 149

[Close]

BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.9e-54
Identity = 93/144 (64.58%), Postives = 114/144 (79.17%), Query Frame = 0


Query: 12  LLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYND 71
           ++L    S+   S+DFDFFYFVQQWPG+YCDT Q++CCYP +G+PAADFGIHGLWPNY D
Sbjct: 15  VILPSVFSASSSSEDFDFFYFVQQWPGSYCDT-QKKCCYPNSGKPAADFGIHGLWPNYKD 74

Query: 72  GTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELDQ 131
           GTYPSNCD    FD + IS++L+SM+K WP+L+CPS +G  FW HEWEKHGTCSES +DQ
Sbjct: 75  GTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQ 134

Query: 132 KEYFEAAIKLKEKANLLKLLNNAG 156
            EYF+ A+ LK+K NLL  L  AG
Sbjct: 135 HEYFQTALNLKQKTNLLGALTKAG 157

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BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 3.0e-52
Identity = 91/148 (61.49%), Postives = 118/148 (79.73%), Query Frame = 0


Query: 9   IFKLLLLQCLSSLCL-SQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWP 68
           + K++++QCL  LC+ SQDFDFFYFVQQWP +YCDT++  CCYP TG+P  DF IHGLWP
Sbjct: 9   LIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRS-CCYPTTGKPDEDFSIHGLWP 68

Query: 69  NYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSES 128
           NY DG +P NCD +S  D +E S+++S+M+K+WPSL+CPSS+GL+FWSHEW KHGTC  S
Sbjct: 69  NYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--S 128

Query: 129 ELDQKEYFEAAIKLKEKANLLKLLNNAG 156
            L+Q  YF+ A+  K K+NLL+ LNNAG
Sbjct: 129 ALNQHAYFQTALDFKTKSNLLQNLNNAG 153

[Close]

BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.8e-24
Identity = 64/141 (45.39%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 25  QDFDFFYFVQQWPGAYCDTKQQRC----CYPKTGRPAADFGIHGLWPNYNDGTYPSNCDP 84
           ++FD+F    QWPG YC   +  C    C   +  P   F IHGLWP+YNDG++PS C  
Sbjct: 30  REFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAP-TQFTIHGLWPDYNDGSWPS-CCY 89

Query: 85  DSLFDRTEISEVLSSMQKHWPSLSC--PSS-NGLR--FWSHEWEKHGTCSESEL-DQKEY 144
            S F   EIS ++  ++K+WPSLSC  PSS NG +  FW HEWEKHGTCS     D+  Y
Sbjct: 90  RSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNY 149

Query: 145 FEAAIKLKEKANLLKLLNNAG 156
           F   + L  K N+  +L  AG
Sbjct: 150 FLTTLNLYLKHNVTDVLYQAG 168

[Close]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL
Match: A0A5A7SY93 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004270 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.0e-79
Identity = 140/155 (90.32%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MK  YSIFIF LLLLQ LSSLCLSQDFDFFYFVQQWPGAYCDTK+Q CCYPKTGRP ADF
Sbjct: 1   MKFNYSIFIFNLLLLQPLSSLCLSQDFDFFYFVQQWPGAYCDTKKQHCCYPKTGRPVADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DGTYPS+C+PDS+FDRT+ISEVLSSM+KHWPSLSCPSSNGLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGTYPSDCNPDSVFDRTQISEVLSSMEKHWPSLSCPSSNGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLL +LNNAG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLNVLNNAG 155

[Close]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL
Match: A0A1S3BEW0 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489123 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.0e-79
Identity = 140/155 (90.32%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MK  YSIFIF LLLLQ LSSLCLSQDFDFFYFVQQWPGAYCDTK+Q CCYPKTGRP ADF
Sbjct: 1   MKFNYSIFIFNLLLLQPLSSLCLSQDFDFFYFVQQWPGAYCDTKKQHCCYPKTGRPVADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DGTYPS+C+PDS+FDRT+ISEVLSSM+KHWPSLSCPSSNGLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGTYPSDCNPDSVFDRTQISEVLSSMEKHWPSLSCPSSNGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLL +LNNAG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLNVLNNAG 155

[Close]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL
Match: A0A0A0KVY9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605150 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.6e-79
Identity = 139/155 (89.68%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MK  YSIFIF LL+LQ LS+LCLSQDFDFFYFVQQWPGAYCDTK+Q CCYP+TGRPAADF
Sbjct: 1   MKFNYSIFIFNLLILQHLSTLCLSQDFDFFYFVQQWPGAYCDTKRQHCCYPETGRPAADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG+YPSNCDPDS+FDRT+ISEVLSSM KHWPSLSCPSSNGLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGSYPSNCDPDSVFDRTQISEVLSSMDKHWPSLSCPSSNGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLLK+LN+AG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKVLNSAG 155

[Close]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL
Match: A0A6J1H0Y9 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458576 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.6e-75
Identity = 134/155 (86.45%), Postives = 145/155 (93.55%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MKP YSIFIF LLLLQ LS LCL+QDFDFFYFVQQWPGAYC++KQQRCCYPKTGRP+ADF
Sbjct: 1   MKPSYSIFIFNLLLLQYLSPLCLAQDFDFFYFVQQWPGAYCNSKQQRCCYPKTGRPSADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG+YPSNC+ +S FDRT+ISE LSSMQK WPSLSCPSS+GLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGSYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAI+LKEKANLLKLL +AG
Sbjct: 121 HGTCSESELDQKEYFEAAIRLKEKANLLKLLYDAG 155

[Close]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL
Match: A0A6J1K2J7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111489512 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.4e-75
Identity = 134/155 (86.45%), Postives = 143/155 (92.26%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MKP YSIFIF LLLLQ LS LCL+QDFDFFYFVQQWPGAYC++KQ RCCYPKTGRP+ADF
Sbjct: 1   MKPSYSIFIFNLLLLQYLSPLCLAQDFDFFYFVQQWPGAYCNSKQHRCCYPKTGRPSADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG YPSNC+ +S FDRT+ISE LSSMQK WPSLSCPSS+GLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGGYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLL LLN+AG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLNLLNDAG 155

[Close]

BLAST of Lsi04G004230 vs. NCBI nr
Match: XP_038893027.1 (ribonuclease 3-like [Benincasa hispida])

HSP 1 Score: 307.8 bits (787), Expect = 5.5e-80
Identity = 143/156 (91.67%), Postives = 151/156 (96.79%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSS-LCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MKP YSIFIFKLLLLQ LSS LCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD
Sbjct: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60

Query: 61  FGIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNY DG+YPSNCD +S+FDRT+IS+VLSSM+KHWPSLSCPSSNGLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120

Query: 121 KHGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           KHGTCSESELDQ+EYFEAA+KLKEKANLLKLLNNAG
Sbjct: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAG 156

[Close]

BLAST of Lsi04G004230 vs. NCBI nr
Match: XP_008446356.1 (PREDICTED: ribonuclease 3-like [Cucumis melo] >KAA0034421.1 ribonuclease 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 303.5 bits (776), Expect = 1.0e-78
Identity = 140/155 (90.32%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MK  YSIFIF LLLLQ LSSLCLSQDFDFFYFVQQWPGAYCDTK+Q CCYPKTGRP ADF
Sbjct: 1   MKFNYSIFIFNLLLLQPLSSLCLSQDFDFFYFVQQWPGAYCDTKKQHCCYPKTGRPVADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DGTYPS+C+PDS+FDRT+ISEVLSSM+KHWPSLSCPSSNGLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGTYPSDCNPDSVFDRTQISEVLSSMEKHWPSLSCPSSNGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLL +LNNAG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLNVLNNAG 155

[Close]

BLAST of Lsi04G004230 vs. NCBI nr
Match: XP_004135175.1 (ribonuclease 3 [Cucumis sativus])

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 139/155 (89.68%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MK  YSIFIF LL+LQ LS+LCLSQDFDFFYFVQQWPGAYCDTK+Q CCYP+TGRPAADF
Sbjct: 1   MKFNYSIFIFNLLILQHLSTLCLSQDFDFFYFVQQWPGAYCDTKRQHCCYPETGRPAADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG+YPSNCDPDS+FDRT+ISEVLSSM KHWPSLSCPSSNGLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGSYPSNCDPDSVFDRTQISEVLSSMDKHWPSLSCPSSNGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLLK+LN+AG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKVLNSAG 155

[Close]

BLAST of Lsi04G004230 vs. NCBI nr
Match: XP_023549036.1 (ribonuclease 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-76
Identity = 136/155 (87.74%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MKP YSIFIF LLLLQ LS LCL+QDFDFFYFVQQWPGAYC++KQQRCCYPKTGRP+ADF
Sbjct: 1   MKPSYSIFIFNLLLLQYLSPLCLAQDFDFFYFVQQWPGAYCNSKQQRCCYPKTGRPSADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG+YPSNC+ +S FDRT+ISE LSSMQK WPSLSCPSS+GLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGSYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAIKLKEKANLLKLLN+AG
Sbjct: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNDAG 155

[Close]

BLAST of Lsi04G004230 vs. NCBI nr
Match: XP_022957089.1 (ribonuclease 3-like [Cucurbita moschata])

HSP 1 Score: 291.2 bits (744), Expect = 5.4e-75
Identity = 134/155 (86.45%), Postives = 145/155 (93.55%), Query Frame = 0


Query: 1   MKPYYSIFIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADF 60
           MKP YSIFIF LLLLQ LS LCL+QDFDFFYFVQQWPGAYC++KQQRCCYPKTGRP+ADF
Sbjct: 1   MKPSYSIFIFNLLLLQYLSPLCLAQDFDFFYFVQQWPGAYCNSKQQRCCYPKTGRPSADF 60

Query: 61  GIHGLWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEK 120
           GIHGLWPNY DG+YPSNC+ +S FDRT+ISE LSSMQK WPSLSCPSS+GLRFWSHEWEK
Sbjct: 61  GIHGLWPNYKDGSYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWEK 120

Query: 121 HGTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           HGTCSESELDQKEYFEAAI+LKEKANLLKLL +AG
Sbjct: 121 HGTCSESELDQKEYFEAAIRLKEKANLLKLLYDAG 155

[Close]

BLAST of Lsi04G004230 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 225.7 bits (574), Expect = 2.6e-59
Identity = 99/147 (67.35%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 9   IFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPN 68
           + KLL+ Q L      +DFDFFYFV QWPGAYCDT  + CCYP +G+PAADFGIHGLWPN
Sbjct: 10  LLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDT-SRACCYPTSGKPAADFGIHGLWPN 69

Query: 69  YNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSESE 128
           YN G++PSNCDPDS FDR++IS+++SS++K+WP+LSCPS+ G  FW HEWEKHGTCSES 
Sbjct: 70  YNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESV 129

Query: 129 LDQKEYFEAAIKLKEKANLLKLLNNAG 156
           +DQ EYFE A+KLK+KANLL++L N+G
Sbjct: 130 MDQHEYFENALKLKQKANLLQILKNSG 155

[Close]

BLAST of Lsi04G004230 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 219.5 bits (558), Expect = 1.8e-57
Identity = 97/148 (65.54%), Postives = 121/148 (81.76%), Query Frame = 0


Query: 8   FIFKLLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWP 67
           FIF +L LQ L     +QDFDFFYFV QWPGAYCD++   CCYP+TG+PAADFGIHGLWP
Sbjct: 4   FIF-ILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHS-CCYPQTGKPAADFGIHGLWP 63

Query: 68  NYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSES 127
           NY  G +P NC+PDS FD   +S+++S +Q+ WP+LSCPS++G++FW+HEWEKHGTC+ES
Sbjct: 64  NYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAES 123

Query: 128 ELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           ELDQ +YFEA +KLK+KANLL  L NAG
Sbjct: 124 ELDQHDYFEAGLKLKQKANLLHALTNAG 149

[Close]

BLAST of Lsi04G004230 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 213.4 bits (542), Expect = 1.3e-55
Identity = 93/144 (64.58%), Postives = 114/144 (79.17%), Query Frame = 0


Query: 12  LLLLQCLSSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYND 71
           ++L    S+   S+DFDFFYFVQQWPG+YCDT Q++CCYP +G+PAADFGIHGLWPNY D
Sbjct: 15  VILPSVFSASSSSEDFDFFYFVQQWPGSYCDT-QKKCCYPNSGKPAADFGIHGLWPNYKD 74

Query: 72  GTYPSNCDPDSLFDRTEISEVLSSMQKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELDQ 131
           GTYPSNCD    FD + IS++L+SM+K WP+L+CPS +G  FW HEWEKHGTCSES +DQ
Sbjct: 75  GTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQ 134

Query: 132 KEYFEAAIKLKEKANLLKLLNNAG 156
            EYF+ A+ LK+K NLL  L  AG
Sbjct: 135 HEYFQTALNLKQKTNLLGALTKAG 157

[Close]

BLAST of Lsi04G004230 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 144.8 bits (364), Expect = 5.8e-35
Identity = 74/154 (48.05%), Postives = 96/154 (62.34%), Query Frame = 0


Query: 7   IFIFKLLLLQCL--SSLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHG 66
           I I  LL+LQ L  SS     DF+FFY+V  WPGA CD+ Q+ CC P  G  A+DF IHG
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDS-QKGCCPPTKGNTASDFIIHG 63

Query: 67  LWPNYNDGTYPSNCDPDSLFDRTEISEVLSSMQKHWPS---LSCPSSNGLRFWSHEWEKH 126
           LWP +N+GT+P+ CD  +LFD ++IS+++  M+K W      +CP SN    W HEW KH
Sbjct: 64  LWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACP-SNETNLWEHEWNKH 123

Query: 127 GTCSESELDQKEYFEAAIKLKEKANLLKLLNNAG 156
           GTC +S  DQ  YF   +K K K +LL +L   G
Sbjct: 124 GTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKG 155

[Close]

BLAST of Lsi04G004230 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 112.1 bits (279), Expect = 4.1e-25
Identity = 64/141 (45.39%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 25  QDFDFFYFVQQWPGAYCDTKQQRC----CYPKTGRPAADFGIHGLWPNYNDGTYPSNCDP 84
           ++FD+F    QWPG YC   +  C    C   +  P   F IHGLWP+YNDG++PS C  
Sbjct: 30  REFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAP-TQFTIHGLWPDYNDGSWPS-CCY 89

Query: 85  DSLFDRTEISEVLSSMQKHWPSLSC--PSS-NGLR--FWSHEWEKHGTCSESEL-DQKEY 144
            S F   EIS ++  ++K+WPSLSC  PSS NG +  FW HEWEKHGTCS     D+  Y
Sbjct: 90  RSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNY 149

Query: 145 FEAAIKLKEKANLLKLLNNAG 156
           F   + L  K N+  +L  AG
Sbjct: 150 FLTTLNLYLKHNVTDVLYQAG 168

The following BLAST results are available for this feature:

BLAST of Lsi04G004230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P80022	2.6e-56	64.24	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	2.6e-56	65.54	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P42813	1.9e-54	64.58	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	3.0e-52	61.49	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42814	5.8e-24	45.39	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]

BLAST of Lsi04G004230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SY93	5.0e-79	90.32	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004...	[more]
A0A1S3BEW0	5.0e-79	90.32	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489123 PE=3 SV=1	[more]
A0A0A0KVY9	6.6e-79	89.68	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605150 PE=3 SV=1	[more]
A0A6J1H0Y9	2.6e-75	86.45	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458576 PE=3 SV=1	[more]
A0A6J1K2J7	4.4e-75	86.45	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111489512 PE=3 SV=1	[more]

BLAST of Lsi04G004230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893027.1	5.5e-80	91.67	ribonuclease 3-like [Benincasa hispida]	[more]
XP_008446356.1	1.0e-78	90.32	PREDICTED: ribonuclease 3-like [Cucumis melo] >KAA0034421.1 ribonuclease 3-like ...	[more]
XP_004135175.1	1.4e-78	89.68	ribonuclease 3 [Cucumis sativus]	[more]
XP_023549036.1	2.8e-76	87.74	ribonuclease 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957089.1	5.4e-75	86.45	ribonuclease 3-like [Cucurbita moschata]	[more]

BLAST of Lsi04G004230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	2.6e-59	67.35	Ribonuclease T2 family protein	[more]
AT1G26820.1	1.8e-57	65.54	ribonuclease 3	[more]
AT2G02990.1	1.3e-55	64.58	ribonuclease 1	[more]
AT1G14210.1	5.8e-35	48.05	Ribonuclease T2 family protein	[more]
AT2G39780.1	4.1e-25	45.39	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 21..157 e-value: 7.0E-54 score: 185.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 22..156
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 27..155 e-value: 1.1E-41 score: 143.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 6..155
None	No IPR available	PANTHER	PTHR11240:SF55	F7A19.32 PROTEIN	coord: 6..155
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 60..67
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 113..124
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 27..155 e-value: 8.09291E-55 score: 169.435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G004230.1	Lsi04G004230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding