Lsi04G004180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G004180
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	chr04: 4034742 .. 4038033 (-)
RNA-Seq Expression	Lsi04G004180
Synteny	Lsi04G004180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCCAACCACACCCTTCTGAGCTTTCTTCTGCCCTCGAAAGGCTTGTTCTCAACAGAGACGTCTCTCTCATAGCACCCCTTTTCTTGGGTTTCGCCTCTATGATCCGGACATCCCAAAATCAAGAAATTGAAGCAACACCCACTACTTTGCCTGATCGATTCATCTTCTACAATCCCTTTGCCCATCAATTGATGGTGATCCAAGCCACTCCCAAGGACGGCCAACCCCCAGCCTCTAAAGCCTCCATTAAAGCCATGCCCTCTGTCCCTGTTTCTGAACTTACTGAATGTGTGATTTGTTTGGAGGAGATTGAAGTCGGTGGTCTCGCTAAACAAATGCCCTGTAACCATAAATTCCATGGTGATTGCATACAGAAATGGCTGAAGTTACATGGGTCGTGCCCTGTTTGTAGATATCAGATGCCAATTGATGGTGAAGATGAAGGGAAAAAGGTTGGTGATGAAGGGACAGAGAGTAGGGGAGAAACAGAGATTTGGGTGAGTTTTTCTTTTGATNAGGTATGATATGAATTGAATTCAACCCAACCCATCCCATTATAGACGTTTGACGACAACGTTTCACCAGATGGTTTAAAAAAAGAATTCTTTTTTTATGTATTTTGACAAATAAATAAACTCAAATTATTATTTTTTTAAAAGAAAAATATTAAAGAAATTGAATTTACCCTTTATTTTTAAAATATTTTTGTTCTTTCTTAAGTTACAATATTACTCTTGTTCTTTTATAAACGTTTCAAAACTTAATTCATTAAAATGTTAGATGATAATTGGGACTTGGAATAGTGTGTTGGCAACTTGAAATAAAAAATTGAAACTAGAACACCAACAAACGGTTCTAGGTCAATAACACATTTGTTCTAAGTTTCTAATTTTGTTTAATATTTTAATAAATTTCTTCTCCAATTGTAAGTTTGAAATGCTTATAAAAGTTTAAAGTTATTGTAGTAATTTTTGAAAAAAGTATAGGTATTTGTTAAACAAAAGTGAAATATATAAGGGTTGTTTGGTTGGTAATCCATATTCTATTTTATGTTTTCGACAAATATGTGTTTGACAGACAATTTAGATTTTGTTTTTTAAAACACTTTTGGATTATGTATCTAAACAGTTTAAATTTTGAAACCAATATTTTTATATTGTAAGTGTATTCAAATTTTAAAATGCAGAATTATATTAGATAAAGATAAAAACATTTAAAAATATGGTAGTTCATGTTATATACTCAATTCAATTTTTAAAATTAAAATATATAGTATGTAATGTATTGTAAATTATAAAATATTATAAACACAATAATTATATAAAATTTTTTTAACATAAAACTAGTTTATAGATAATACAACTAGTTTTATGGTTTATAAATATAATATCAAACAAATTTAAAATAATTGTTTAAATTAGAATCTAATTTCTTTGAGAATATGCTACCAAACACATATATGAAAACTTTTCATTGAATCTATTATTTTCAAATTTTTGTTTTTAGATTCTCTACCAAACCTATCCTAAGAGAATTTTTTTTTTAATTTTTAAATTTTTTAATATATATATATATATATATTTATTGTAATTTAAGGAAAAGAAACCAGGTAATGACATAGTTTTAGGTTGATGTTGAGTAGGAGTGTTCAAATGATCTGACAACTCGAACAATCCGGACTATCCAACCCAAAATGTAAGTATTGAGTTGGATTAGTTTTGTTCTTGGGTTGTATGGATTAATTTGTGAATTTTTTATATTTTTCTTTTAAAATTGTTATGATATTTATCTTTGTTTAAAGCTTATAATAATATTAGAGATATTTGGTATTTAACATTTAATTTTTATGAAATGTTATAGATAAATTTATATATTTTTTCTTAAAAAATAAAATTATAAACCAATAATCCAAACCAACCTAGATAGACTAGTAATGTTAGAGATTTTGTGTAAGGATGGAAAATGGAACTGCCGTCCAAGGCCAGCTATACTTTTTATTCCAAAATGTATATTAAAAGTGAAAACTTCGAGATAAGACTCTTCGTTAAAAGAAAAGATAAAAGAAAAAAGAAACGAACGACTCTTCTTCCAAAAAGTATCCAACCAGAATCAGCCACGTCAAACGGTAAATTTACCTTCTTTATAAAGATGGCTTTACGCTATTGGACTGTTGCGGAGAGAGGACGACCGACCGGGTTACACCCGAAGATAAGTTCCCTTCCCCGGTTCCCCCTCTTTTTAACTCTTTTTAAAATTATCAATTAAACGGTATCGTATCATTTTTTATTTATTTTTAATTTTAATTAAATGTAACGACAACTATTCCTATTGCCTTAAATTAATTTATGAAATTTCAAGCTTGAAGATCTGGGACTTGTTCAATATTGTTCTCCTTTTTCTCCTTTTTTTCTCTTTTTATATTTATTTGTTTTTCTATGCTTTGACGTCGACTCTAAGCTTGAAACGACAGCGATCTATTACTCACAGCAACCTCCCCTTTCTCTCTCTTTCTCCCTCATTTTCTTGCTTCTCCTTCTTCTTCCTCCTCCACCGTCTTGCCTTTGCTTTCATTTTCTCTGCATTTCACTCCGGCCGGACATCCTCTTCACATTTTCAAGGTAAATTTGCTACCTCTCTCTCTCTCTACTTTTCCTGTATACGTTCTTCTCCGATGAACTCTTGAGGTTTTCAGTGCTCTTCTATTGAACGGCTATACAATGTCTTTGGATCTCGTTGTGATTTCCCTACTTGATGAAGAACACGGTGTTTTGCTTTTCTACTTCTTCCTTTACTTTCCTCTTTGTTTTGCTTTTTGTTTTTATACTTGTTTCAGGTTCGAGAAAATTTTACCTGGTGAAGTGCGGATGAATTGTTTGTTGTTGTTTCTCTTCAGTCTTCGATTTCTTTTACTTCTCCATTTTCCTTTCTGGTACTGGTACTGGTACTGTTACTAATTCTATTTTGAGTTTAGTAATTTTGTTTTTGAGAAAATGTTTTGTTGCGATTTACGCGGGATGTAAGTTTGGAAAATTTATGTTAGCATTAATTGCAGAAACTAGTTGCCCTACTCTCTGATACATTGAGTAGTATAGTATTGATTTGATATTCTGCAATTAATGCATGTCGAAAGTCTGATTTAACTTATATCCACCATTTGTTCTAAGAGATATCTGTTCTTTATTGTCAATTTTTTTCCATGATTTCAGAATTAGTCTCGAGGAAGAAGGTTCCAGTTATTGGCATTTTAGTTTCGTGATGCTTAATGACGGTGGAATTTGGGATTAG

mRNA sequence

ATGGCTTCCCAACCACACCCTTCTGAGCTTTCTTCTGCCCTCGAAAGGCTTGTTCTCAACAGAGACGTCTCTCTCATAGCACCCCTTTTCTTGGGTTTCGCCTCTATGATCCGGACATCCCAAAATCAAGAAATTGAAGCAACACCCACTACTTTGCCTGATCGATTCATCTTCTACAATCCCTTTGCCCATCAATTGATGGTGATCCAAGCCACTCCCAAGGACGGCCAACCCCCAGCCTCTAAAGCCTCCATTAAAGCCATGCCCTCTGTCCCTGTTTCTGAACTTACTGAATGTGTGATTTGTTTGGAGGAGATTGAAGTCGGTGGTCTCGCTAAACAAATGCCCTGTAACCATAAATTCCATGGTGATTGCATACAGAAATGGCTGAAGTTACATGGGTCGTGCCCTGTTTGTAGATATCAGATGCCAATTGATGGTGAAGATGAAGGGAAAAAGGTTGGTGATGAAGGGACAGAGAGTAGGGGAGAAACAGAGATTTGGGTGAGTTTTTCTTTTGATNAGCGATCTATTACTCACAGCAACCTCCCCTTTCTCTCTCTTTCTCCCTCATTTTCTTGCTTCTCCTTCTTCTTCCTCCTCCACCGTCTTGCCTTTGCTTTCATTTTCTCTGCATTTCACTCCGGCCGGACATCCTCTTCACATTTTCAAGTCTTCGATTTCTTTTACTTCTCCATTTTCCTTTCTGGTACTGGTACTGGTACTGTTACTAATTCTATTTTGAGTTTAGTAATTTTGTTTTTGAGAAAATGTTTTGTTGCGATTTACGCGGGATGTAAAATTAGTCTCGAGGAAGAAGGTTCCAGTTATTGGCATTTTAGTTTCGTGATGCTTAATGACGGTGGAATTTGGGATTAG

Coding sequence (CDS)

ATGGCTTCCCAACCACACCCTTCTGAGCTTTCTTCTGCCCTCGAAAGGCTTGTTCTCAACAGAGACGTCTCTCTCATAGCACCCCTTTTCTTGGGTTTCGCCTCTATGATCCGGACATCCCAAAATCAAGAAATTGAAGCAACACCCACTACTTTGCCTGATCGATTCATCTTCTACAATCCCTTTGCCCATCAATTGATGGTGATCCAAGCCACTCCCAAGGACGGCCAACCCCCAGCCTCTAAAGCCTCCATTAAAGCCATGCCCTCTGTCCCTGTTTCTGAACTTACTGAATGTGTGATTTGTTTGGAGGAGATTGAAGTCGGTGGTCTCGCTAAACAAATGCCCTGTAACCATAAATTCCATGGTGATTGCATACAGAAATGGCTGAAGTTACATGGGTCGTGCCCTGTTTGTAGATATCAGATGCCAATTGATGGTGAAGATGAAGGGAAAAAGGTTGGTGATGAAGGGACAGAGAGTAGGGGAGAAACAGAGATTTGGGTGAGTTTTTCTTTTGATNAGCGATCTATTACTCACAGCAACCTCCCCTTTCTCTCTCTTTCTCCCTCATTTTCTTGCTTCTCCTTCTTCTTCCTCCTCCACCGTCTTGCCTTTGCTTTCATTTTCTCTGCATTTCACTCCGGCCGGACATCCTCTTCACATTTTCAAGTCTTCGATTTCTTTTACTTCTCCATTTTCCTTTCTGGTACTGGTACTGGTACTGTTACTAATTCTATTTTGAGTTTAGTAATTTTGTTTTTGAGAAAATGTTTTGTTGCGATTTACGCGGGATGTAAAATTAGTCTCGAGGAAGAAGGTTCCAGTTATTGGCATTTTAGTTTCGTGATGCTTAATGACGGTGGAATTTGGGATTAG

Protein sequence

MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFDXRSITHSNLPFLSLSPSFSCFSFFFLLHRLAFAFIFSAFHSGRTSSSHFQVFDFFYFSIFLSGTGTGTVTNSILSLVILFLRKCFVAIYAGCKISLEEEGSSYWHFSFVMLNDGGIWD

Homology

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BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.5e-33
Identity = 77/181 (42.54%), Postives = 105/181 (58.01%), Query Frame = 0


Query: 8   SELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYNPFAHQLM 67
           +E SS   R + NRD+ L  P  LGF+    ++ + +  A+     +R I  NPF   ++
Sbjct: 11  TETSSVSGRFLRNRDLYLFLPFLLGFSDQESSNGDDDDVASSR---ERIILVNPFTQGMI 70

Query: 68  VIQATP-------------KDGQPPASKASIKAMPSVPVSELT-ECVICLEEIEVGGLAK 127
           V++ +              ++G+PPASKASI AMP V +     ECVICLEE +     K
Sbjct: 71  VLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVK 130

Query: 128 QMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSF 175
           +MPC H+FHG CI+KWL  HGSCPVCRY+MP+DG++ GKK  D         EIWV FSF
Sbjct: 131 EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRND-------GNEIWVRFSF 181

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BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.9e-21
Identity = 42/91 (46.15%), Postives = 62/91 (68.13%), Query Frame = 0


Query: 74  KDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHKFHGDCIQKWLKLH 133
           ++G PPA K +++A+P+V + E+  C +CL+++EVG  AKQMPC HKFH  CI  WL+LH
Sbjct: 174 RNGTPPAKKEAVEALPTVKIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELH 233

Query: 134 GSCPVCRYQMPIDGEDEGKKVGDEGTESRGE 165
            SCPVCR+++P    +E K + +     R E
Sbjct: 234 SSCPVCRFELP---SEETKDLNEPSNIGRVE 261

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BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 8.6e-21
Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 0


Query: 58  FYNPFAHQLMVIQATPKD----GQPPASKASIKAMPSVPV------SELTECVICLEEIE 117
           F+ P   QL + Q    D    G PPASK++I A+P+V V      SE+ +C +C++E E
Sbjct: 166 FFGPGLEQL-IQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE 225

Query: 118 VGGLAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGED-EGKKVGDEGT-ESRGET 167
            G   KQMPC H FH DC+  WL+LH SCPVCR+++P D  D E +  G +G+ + +G  
Sbjct: 226 DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSV 285

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BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.3e-20
Identity = 52/122 (42.62%), Postives = 73/122 (59.84%), Query Frame = 0


Query: 49  PTTLPDRFIFYNPFAHQLMVIQATPKD----GQPPASKASIKAMPSVPV------SELTE 108
           P  + D FI   P   QL + Q    D    G PPASK++I+A+P V +      SE  +
Sbjct: 167 PANIGDYFI--GPGLEQL-IQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQ 226

Query: 109 CVICLEEIEVGGLAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKV-GDE 160
           C +C+++ E G  AKQMPC H +H DC+  WL+LH SCPVCR+++P D  D  ++V G +
Sbjct: 227 CAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQ 285

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BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 38/95 (40.00%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 58  FYNPFAHQL--MVIQATPKDGQPPASKASIKAMPSVPVSEL------TECVICLEEIEVG 117
           F+ P   +L   +   T + G PPA +++I A+P++ +++       + C +C +E E+G
Sbjct: 141 FFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELG 200

Query: 118 GLAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMP 145
             AKQMPCNH +H DCI  WL  H SCPVCR ++P
Sbjct: 201 SEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

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BLAST of Lsi04G004180 vs. ExPASy TrEMBL
Match: A0A5D3D3N1 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00370 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 6.5e-88
Identity = 156/174 (89.66%), Postives = 169/174 (97.13%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MAS P PS++SSALERL+LNRDVSLIAPLFLGFASMIR+SQN E E TPTTLPDRFIF+N
Sbjct: 1   MASPPDPSDISSALERLLLNRDVSLIAPLFLGFASMIRSSQNHENEPTPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PF+HQLMV+QATPKDGQPPASKASIKAMPS+PVSE+TECVICL+EIEVGGLAKQMPCNHK
Sbjct: 61  PFSHQLMVVQATPKDGQPPASKASIKAMPSLPVSEVTECVICLDEIEVGGLAKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHG+CIQKWL+LHGSCPVCRYQMPIDG+DEGKKVGDEGTESRGETEIWVSFSFD
Sbjct: 121 FHGECIQKWLELHGSCPVCRYQMPIDGDDEGKKVGDEGTESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. ExPASy TrEMBL
Match: A0A1S3BFQ2 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103489128 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 6.5e-88
Identity = 156/174 (89.66%), Postives = 169/174 (97.13%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MAS P PS++SSALERL+LNRDVSLIAPLFLGFASMIR+SQN E E TPTTLPDRFIF+N
Sbjct: 1   MASPPDPSDISSALERLLLNRDVSLIAPLFLGFASMIRSSQNHENEPTPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PF+HQLMV+QATPKDGQPPASKASIKAMPS+PVSE+TECVICL+EIEVGGLAKQMPCNHK
Sbjct: 61  PFSHQLMVVQATPKDGQPPASKASIKAMPSLPVSEVTECVICLDEIEVGGLAKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHG+CIQKWL+LHGSCPVCRYQMPIDG+DEGKKVGDEGTESRGETEIWVSFSFD
Sbjct: 121 FHGECIQKWLELHGSCPVCRYQMPIDGDDEGKKVGDEGTESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. ExPASy TrEMBL
Match: A0A0A0KU18 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605210 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 3.6e-86
Identity = 153/174 (87.93%), Postives = 167/174 (95.98%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MAS P PS+L+SALERL+LNRDVSLIAPLFLGFASM+R+SQNQE + TPTTLPDRFIF+N
Sbjct: 1   MASPPDPSDLASALERLLLNRDVSLIAPLFLGFASMVRSSQNQESDPTPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PF+HQLMV+QATPK GQPPASKASIKAMPS+PVSE+TECVICL+EIEVG LAKQMPCNHK
Sbjct: 61  PFSHQLMVVQATPKHGQPPASKASIKAMPSLPVSEVTECVICLDEIEVGRLAKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHGDCIQKWL+LHGSCPVCRYQMPIDG+DEGKKVGDEG ESRGETEIWVSFSFD
Sbjct: 121 FHGDCIQKWLELHGSCPVCRYQMPIDGDDEGKKVGDEGAESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. ExPASy TrEMBL
Match: A0A6J1GWR8 (E3 ubiquitin-protein ligase RING1-like OS=Cucurbita moschata OX=3662 GN=LOC111458226 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 5.0e-72
Identity = 140/179 (78.21%), Postives = 155/179 (86.59%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLV-LNRDVSLIAPLFLGFASMIRT----SQNQEIEATPTTLPDR 60
           MAS P  SELSSA E LV +NRD+S IAPLFLGFAS+IR+    S N EI+  P TLP+R
Sbjct: 1   MASDPVASELSSAFESLVFINRDISPIAPLFLGFASVIRSADPQSPNPEIQTIP-TLPNR 60

Query: 61  FIFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQM 120
           FIF+NPF  QLMVIQATPK+GQPPASKASIKAMPS+PVSELTECVICLEEIE G LAKQM
Sbjct: 61  FIFFNPFPPQLMVIQATPKEGQPPASKASIKAMPSLPVSELTECVICLEEIEAGDLAKQM 120

Query: 121 PCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           PC+HKFHGDCI+KWL+LHGSCP+CRYQMPID EDEGKKV +EG+E RGE EIWVSFSFD
Sbjct: 121 PCSHKFHGDCIEKWLELHGSCPICRYQMPIDDEDEGKKVDEEGSERRGEREIWVSFSFD 178

[Close]

BLAST of Lsi04G004180 vs. ExPASy TrEMBL
Match: A0A6J1HTX2 (E3 ubiquitin-protein ligase RING1-like OS=Cucurbita maxima OX=3661 GN=LOC111467433 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.7e-70
Identity = 136/179 (75.98%), Postives = 152/179 (84.92%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRT----SQNQEIEATPTTLPDRF 60
           M S+P PSELSSALE L+LN DVSL+AP FLGFA+MI T    S NQ IE  P TLPDRF
Sbjct: 1   MPSEPDPSELSSALETLILNTDVSLLAPHFLGFATMIHTIAPQSPNQGIETNP-TLPDRF 60

Query: 61  IFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMP 120
           IF+NPF HQL++IQ+TPK+ QPPASKASI AMPSV VS  TECVICL+EIEVGG+AK+MP
Sbjct: 61  IFFNPFLHQLIMIQSTPKNSQPPASKASIIAMPSVHVSAQTECVICLDEIEVGGVAKEMP 120

Query: 121 CNHKFHGDCIQKWLKLHGSCPVCRYQMPI-DGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           CNHKFHGDCI+KWL+LHGSCPVCRY+MP+ D EDEGKKV DEGTE  GE EIWVSFSFD
Sbjct: 121 CNHKFHGDCIEKWLELHGSCPVCRYEMPVDDDEDEGKKVEDEGTERMGEREIWVSFSFD 178

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BLAST of Lsi04G004180 vs. NCBI nr
Match: XP_038891708.1 (E3 ubiquitin-protein ligase MPSR1-like [Benincasa hispida])

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-90
Identity = 164/174 (94.25%), Postives = 170/174 (97.70%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MASQP PSEL SALERL LNRDVSLIAPLFLGFASMIRTSQ+QEIEATPTTLPDRFIF+N
Sbjct: 1   MASQPDPSELGSALERLFLNRDVSLIAPLFLGFASMIRTSQDQEIEATPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PFAHQL+VIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGL+KQMPCNHK
Sbjct: 61  PFAHQLIVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLSKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHGDCIQKWL+LHGSCPVCRYQMPIDGEDEGKKV +EGTESRGETEIWVSFSFD
Sbjct: 121 FHGDCIQKWLELHGSCPVCRYQMPIDGEDEGKKVDNEGTESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. NCBI nr
Match: XP_008446365.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0034425.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK17709.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 334.0 bits (855), Expect = 1.3e-87
Identity = 156/174 (89.66%), Postives = 169/174 (97.13%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MAS P PS++SSALERL+LNRDVSLIAPLFLGFASMIR+SQN E E TPTTLPDRFIF+N
Sbjct: 1   MASPPDPSDISSALERLLLNRDVSLIAPLFLGFASMIRSSQNHENEPTPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PF+HQLMV+QATPKDGQPPASKASIKAMPS+PVSE+TECVICL+EIEVGGLAKQMPCNHK
Sbjct: 61  PFSHQLMVVQATPKDGQPPASKASIKAMPSLPVSEVTECVICLDEIEVGGLAKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHG+CIQKWL+LHGSCPVCRYQMPIDG+DEGKKVGDEGTESRGETEIWVSFSFD
Sbjct: 121 FHGECIQKWLELHGSCPVCRYQMPIDGDDEGKKVGDEGTESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. NCBI nr
Match: XP_011655684.1 (E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN51927.1 hypothetical protein Csa_009131 [Cucumis sativus])

HSP 1 Score: 328.2 bits (840), Expect = 7.3e-86
Identity = 153/174 (87.93%), Postives = 167/174 (95.98%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYN 60
           MAS P PS+L+SALERL+LNRDVSLIAPLFLGFASM+R+SQNQE + TPTTLPDRFIF+N
Sbjct: 1   MASPPDPSDLASALERLLLNRDVSLIAPLFLGFASMVRSSQNQESDPTPTTLPDRFIFFN 60

Query: 61  PFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQMPCNHK 120
           PF+HQLMV+QATPK GQPPASKASIKAMPS+PVSE+TECVICL+EIEVG LAKQMPCNHK
Sbjct: 61  PFSHQLMVVQATPKHGQPPASKASIKAMPSLPVSEVTECVICLDEIEVGRLAKQMPCNHK 120

Query: 121 FHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           FHGDCIQKWL+LHGSCPVCRYQMPIDG+DEGKKVGDEG ESRGETEIWVSFSFD
Sbjct: 121 FHGDCIQKWLELHGSCPVCRYQMPIDGDDEGKKVGDEGAESRGETEIWVSFSFD 174

[Close]

BLAST of Lsi04G004180 vs. NCBI nr
Match: XP_022956506.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata] >KAG7032220.1 E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281.2 bits (718), Expect = 1.0e-71
Identity = 140/179 (78.21%), Postives = 155/179 (86.59%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLV-LNRDVSLIAPLFLGFASMIRT----SQNQEIEATPTTLPDR 60
           MAS P  SELSSA E LV +NRD+S IAPLFLGFAS+IR+    S N EI+  P TLP+R
Sbjct: 1   MASDPVASELSSAFESLVFINRDISPIAPLFLGFASVIRSADPQSPNPEIQTIP-TLPNR 60

Query: 61  FIFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQM 120
           FIF+NPF  QLMVIQATPK+GQPPASKASIKAMPS+PVSELTECVICLEEIE G LAKQM
Sbjct: 61  FIFFNPFPPQLMVIQATPKEGQPPASKASIKAMPSLPVSELTECVICLEEIEAGDLAKQM 120

Query: 121 PCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           PC+HKFHGDCI+KWL+LHGSCP+CRYQMPID EDEGKKV +EG+E RGE EIWVSFSFD
Sbjct: 121 PCSHKFHGDCIEKWLELHGSCPICRYQMPIDDEDEGKKVDEEGSERRGEREIWVSFSFD 178

[Close]

BLAST of Lsi04G004180 vs. NCBI nr
Match: KAG6601438.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.5 bits (711), Expect = 6.7e-71
Identity = 139/179 (77.65%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLV-LNRDVSLIAPLFLGFASMIRT----SQNQEIEATPTTLPDR 60
           MAS P  SELSSA E LV +NRD+S IAPLFLGFAS+IR+    S N EI+  P TLP+R
Sbjct: 1   MASDPVASELSSAFESLVFINRDISPIAPLFLGFASVIRSADPQSPNPEIQTIP-TLPNR 60

Query: 61  FIFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTECVICLEEIEVGGLAKQM 120
           FIF+NPF  QLMVIQATPK+G PPASKASIKAMPS+PVSELTECVICLEEIE G LAKQM
Sbjct: 61  FIFFNPFPPQLMVIQATPKEGLPPASKASIKAMPSLPVSELTECVICLEEIEAGDLAKQM 120

Query: 121 PCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSFD 175
           PC+HKFHGDCI+KWL+LHGSCP+CRYQMPID EDEGKKV +EG+E RGE EIWVSFSFD
Sbjct: 121 PCSHKFHGDCIEKWLELHGSCPICRYQMPIDDEDEGKKVDEEGSERRGEREIWVSFSFD 178

[Close]

BLAST of Lsi04G004180 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-34
Identity = 77/181 (42.54%), Postives = 105/181 (58.01%), Query Frame = 0


Query: 8   SELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTTLPDRFIFYNPFAHQLM 67
           +E SS   R + NRD+ L  P  LGF+    ++ + +  A+     +R I  NPF   ++
Sbjct: 11  TETSSVSGRFLRNRDLYLFLPFLLGFSDQESSNGDDDDVASSR---ERIILVNPFTQGMI 70

Query: 68  VIQATP-------------KDGQPPASKASIKAMPSVPVSELT-ECVICLEEIEVGGLAK 127
           V++ +              ++G+PPASKASI AMP V +     ECVICLEE +     K
Sbjct: 71  VLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVK 130

Query: 128 QMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVSFSF 175
           +MPC H+FHG CI+KWL  HGSCPVCRY+MP+DG++ GKK  D         EIWV FSF
Sbjct: 131 EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRND-------GNEIWVRFSF 181

[Close]

BLAST of Lsi04G004180 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.6e-22
Identity = 60/184 (32.61%), Postives = 89/184 (48.37%), Query Frame = 0


Query: 1   MASQPHPSELSSALERLVLNRDVSLIAPLFLGFASMIRTSQNQEIEATPTT-----LPDR 60
           M+S+    E SS  ER++  R   L   L +  A   R   + +     TT     L  R
Sbjct: 1   MSSENDFVEFSSMFERIIQGRGDGLSRFLPVIVALAAREDDDDQGSTDQTTRRGDPLSPR 60

Query: 61  FIFYNPFAHQLMVIQATPKDGQPPASKASIKAMPSVPVSELTE-----CVICLEEIEVGG 120
           F+     +          K G+ PA K+ ++ MP V + E  E     C ICL+E   G 
Sbjct: 61  FVMIGSRSGLDDFFSDGGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGD 120

Query: 121 LAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGEDEGKKVGDEGTESRGETEIWVS 175
           +A +MPC HKFH  C+++WL  H +CP+CRY+MP++  +E KK+G           IW+ 
Sbjct: 121 VAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEEKKIG-----------IWIG 173

[Close]

BLAST of Lsi04G004180 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 6.1e-22
Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 0


Query: 20  NRDVSLIAPLFLGFASMIRTSQNQ-EIEATPT-TLPDRFI--FYNPFAHQLMVIQATPKD 79
           N D S +  L   F   IR  Q+  +  + P+ +L D FI   +     +L       + 
Sbjct: 141 NPDNSELVVLINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRY 200

Query: 80  GQPPASKASIKAMPSVPVSE-LTECVICLEEIEVGGLAKQMPCNHKFHGDCIQKWLKLHG 139
           G PPA+K +++A+  V + + L +C +CL++ E+G  AK+MPC HKFH DC+  WL+LH 
Sbjct: 201 GTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHS 260

Query: 140 SCPVCRYQMPIDGEDEGK 153
           SCPVCRY +P   +DE K
Sbjct: 261 SCPVCRYLLPTGDDDEPK 278

[Close]

BLAST of Lsi04G004180 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 6.1e-22
Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 0


Query: 58  FYNPFAHQLMVIQATPKD----GQPPASKASIKAMPSVPV------SELTECVICLEEIE 117
           F+ P   QL + Q    D    G PPASK++I A+P+V V      SE+ +C +C++E E
Sbjct: 166 FFGPGLEQL-IQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE 225

Query: 118 VGGLAKQMPCNHKFHGDCIQKWLKLHGSCPVCRYQMPIDGED-EGKKVGDEGT-ESRGET 167
            G   KQMPC H FH DC+  WL+LH SCPVCR+++P D  D E +  G +G+ + +G  
Sbjct: 226 DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSV 285

[Close]

BLAST of Lsi04G004180 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 6.1e-22
Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 0


Query: 20  NRDVSLIAPLFLGFASMIRTSQNQ-EIEATPT-TLPDRFI--FYNPFAHQLMVIQATPKD 79
           N D S +  L   F   IR  Q+  +  + P+ +L D FI   +     +L       + 
Sbjct: 141 NPDNSELVVLINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRY 200

Query: 80  GQPPASKASIKAMPSVPVSE-LTECVICLEEIEVGGLAKQMPCNHKFHGDCIQKWLKLHG 139
           G PPA+K +++A+  V + + L +C +CL++ E+G  AK+MPC HKFH DC+  WL+LH 
Sbjct: 201 GTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHS 260

Query: 140 SCPVCRYQMPIDGEDEGK 153
           SCPVCRY +P   +DE K
Sbjct: 261 SCPVCRYLLPTGDDDEPK 278

The following BLAST results are available for this feature:

BLAST of Lsi04G004180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.5e-33	42.54	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AVN2	3.9e-21	46.15	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	8.6e-21	42.98	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	7.3e-20	42.62	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	1.3e-16	40.00	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Lsi04G004180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D3N1	6.5e-88	89.66	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BFQ2	6.5e-88	89.66	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103489128 P...	[more]
A0A0A0KU18	3.6e-86	87.93	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605210 P...	[more]
A0A6J1GWR8	5.0e-72	78.21	E3 ubiquitin-protein ligase RING1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1HTX2	2.7e-70	75.98	E3 ubiquitin-protein ligase RING1-like OS=Cucurbita maxima OX=3661 GN=LOC1114674...	[more]

BLAST of Lsi04G004180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891708.1	3.8e-90	94.25	E3 ubiquitin-protein ligase MPSR1-like [Benincasa hispida]	[more]
XP_008446365.1	1.3e-87	89.66	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0034425.1 E...	[more]
XP_011655684.1	7.3e-86	87.93	E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN51927.1 hypothetical pro...	[more]
XP_022956506.1	1.0e-71	78.21	E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata] >KAG7032220.1 E3 ubi...	[more]
KAG6601438.1	6.7e-71	77.65	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi04G004180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	1.1e-34	42.54	RING/U-box superfamily protein	[more]
AT1G14200.1	3.6e-22	32.61	RING/U-box superfamily protein	[more]
AT3G13430.1	6.1e-22	38.41	RING/U-box superfamily protein	[more]
AT3G19950.1	6.1e-22	42.98	RING/U-box superfamily protein	[more]
AT3G13430.2	6.1e-22	38.41	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 99..139 e-value: 3.7E-8 score: 43.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 98..140 e-value: 1.8E-13 score: 50.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 99..140 score: 12.681067
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 68..146 e-value: 7.9E-21 score: 75.5
None	No IPR available	PANTHER	PTHR15710:SF100	RING/U-BOX SUPERFAMILY PROTEIN-RELATED	coord: 67..179
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 67..179
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 98..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G004180.1	Lsi04G004180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity