Lsi04G003720 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G003720
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	t-SNARE coiled-coil homology domain-containing protein
Location	chr04: 3709630 .. 3712738 (+)
RNA-Seq Expression	Lsi04G003720
Synteny	Lsi04G003720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATCGCAATCTGAGAATCCCAACGGTCTAAAATTGTAAGGGAGGACAACCAATTTCAAAGTGAAAATTTTGTATCTTGATTGAACCTTTAATTTTTTTTTTAGCGAGTCAGATCGGAGACGTACAGTTCTCTGCTTTTCCGCTTCGCCTCCCATCAATCTGTTACGGATTTTCAATTTTATCTCTGTTTAAGCATCGAACACGCTGATAATTGTCAAGAACAAAAGCCCCATTATTTCGCTTTCGTTTCATATTTTCTTCCTGCTTCCGATTTTCAGGTGCCATTTCCTCTCATTTTCCCCTTCTTTTCGTTGTTTGTTGATCTTATATTTGTATTTACACTGGCATTTCTGTGGGTTTCCTCCGTTTTTCAACCCTCATTTTCCATTCATATGGTTTATCTGTATCGTTATTGGCTAAATTGCTAAGAATTTCGGTTTCGTTTAACTTTCGATCCCTAATTTTCCGCTTGAATTACATTCTGCATTCTTATTTCCAGTTGCTTGGTAATGTTAATTTGTTTTGTTTACTGCGAATCCGTTGTCCTCTAAAGAGTTAAGTTTCTATTGGAAATGTTGTTCTTACAGTCCTTTCCCTAAATATGCTCAATACTATTTTGCAGGTAACAGTTGTATTCACCAGATCTTTGTTTTAGAGCCGCTTACCCAATAGTTCATTTAAATTCAAGAATCCCTTTGTACAGCCAGGATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGCAGATCCTGAATTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATGCTGGGCCTGAAGCCAAACAAACTCTTAATGGTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAACTCCATGTCCTCTAACCCTTTTGATGATGATGATGATGGTTTTGTTGGAAAAAGGACTGCAACTTCTTCAGCCTCTAAAGACAGGTTCAAAAATGATTTTCGCGACTCGGGAGGACTTGAAAACCAGAGTGTGCAGGAATTGGAAAACTATGCTGCGTATAAAGCTGAGGAGACCACAAAATCTGTTAATAACTGCTTAAAGATTGCTGAGGACATAAGGGAAGATGCTACCAGAACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGTAGGTTCTTTTCTAGTTTCCATCAGACGCCTTTCCCATATATTTTCTGTCCTATGTTGATATCCTGAAATTACTAGTTGGCCCATGTTGATAAGATATATTTGGCTTGCAAGTTTTTGTGATGATTAGATTGTGGATGTCCTACTCTGTCAGAGATGGAAAATGATTAAGAACAAAGAACCTTTTAGTTTGCTAGGAATTCGATAAGCAAGCCAGAGATCGAATGAATATATTTTCGTCTTGCTTTTTCATGTTTATGTGTCCTTTTTTCATGATACCTTGATTGCTCCTCCATGTGTTTGTTAATAGTTTCTTTTATTCATCATTGTTAATTACAGGGTGAGAAGCTTCTGAATAATCTTGGAGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGGTTATATCTTCACTGATTCTTTTGCTCAATAAACATTGTCATTCTTGTATCTATCTTGCTGTCACTCTCATTATCTGCTATTGATTATATCTATGCATTTAGGCTGTCCAGATAATTCCTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGTATCTACGGGCAAAAAACAGTCTCAAACACCACCCTCTGAACCATCAGGTGCAATGCAGAAAGTTGAGGTGAGATCATCGGCTGTTGTTATTTCACTGTCTAAGTGCACGTTTGAGAATGATTTTAAAAATGTACAGATTTAAAATCACTCCAAAAATACACTTTTAATCACTTAAAGCTAATTTAAACTTTTATTTTACACTTTAAAATGCAAATTTCATATCATCAAAGTTGGTTCTAAATGATTAAAAGCATGTTTCAAAATGGTTATGGAAATGACAAAAGTGATTTTAACCATTTCACAATCACTCCCAAACATTGCCTTAAAGTAGAGCATAGCATTATCATGGTTTCTAATTTAACCAATATGTAAGAATGTTAACACAGGCTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGATATGGGAAGCGAGCTTGACAGGTCATACATGCCTTCCTCTTTTCTTAATTTGAATATTGTTTGTATTATGATCTCATCTCATCAATCTGTTAGCTTATTACATAGTTCTGAGTGGATACTTTCTGGAAAATGCAGGCAAAACAAAGCTCTGGATCATCTAAACGACGATGTCGACGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGATCGAGAAGTAAATCAGACACTTGTTCTTTTTCCCACCTCTTCCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGTTTAAACATTCTTTAATTGTTGGTGTACTTTTCTTTTTCTCTTCTTTTTGATCTTGTGGTTTTATAAGGTTGTAATAAGTACAAAGCTTTGCTTCTATTCTTTCAAAGAAAAAGCGTATATCCCCTCATCTTGTTGAAGATAGTTAGCAGCCATGAATTTAGACAATAAGAGCTGAACATTTAGTAGTTGTCACCGTTTGCTTTTCTTTTCTTTTCTTTTTCATGGGATTGACTAAAATGGAAGATAAAGCTACAATACACGAGAGAAAAGGGAAGATGTAAAAGAAAACTCAAATGGAGCTTTTACGTACATATATATTTCCATGATTCAGACAGGACAGCCTATACAGCAAAATTACATTAATTCCATAATCAATAAAAAAGAGATGTGAAAGTTCCATAGTACCCCTCCAAAGAAATAAAACCTCATGAATCCTGCCATGTCGGCATTTTGTACCTTATACCTCCAAAATTAATGAAACTCATGGGTTTCTTTTGAATCTTGCCTCCGGACCCGACCACCTATGACCTTTTTTTTTTAAGAAAAAA

mRNA sequence

AAAATCGCAATCTGAGAATCCCAACGGTCTAAAATTGTAAGGGAGGACAACCAATTTCAAAGTGAAAATTTTGTATCTTGATTGAACCTTTAATTTTTTTTTTAGCGAGTCAGATCGGAGACGTACAGTTCTCTGCTTTTCCGCTTCGCCTCCCATCAATCTGTTACGGATTTTCAATTTTATCTCTGTTTAAGCATCGAACACGCTGATAATTGTCAAGAACAAAAGCCCCATTATTTCGCTTTCGTTTCATATTTTCTTCCTGCTTCCGATTTTCAGGTAACAGTTGTATTCACCAGATCTTTGTTTTAGAGCCGCTTACCCAATAGTTCATTTAAATTCAAGAATCCCTTTGTACAGCCAGGATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGCAGATCCTGAATTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATGCTGGGCCTGAAGCCAAACAAACTCTTAATGGTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAACTCCATGTCCTCTAACCCTTTTGATGATGATGATGATGGTTTTGTTGGAAAAAGGACTGCAACTTCTTCAGCCTCTAAAGACAGGTTCAAAAATGATTTTCGCGACTCGGGAGGACTTGAAAACCAGAGTGTGCAGGAATTGGAAAACTATGCTGCGTATAAAGCTGAGGAGACCACAAAATCTGTTAATAACTGCTTAAAGATTGCTGAGGACATAAGGGAAGATGCTACCAGAACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGGTGAGAAGCTTCTGAATAATCTTGGAGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGGCTGTCCAGATAATTCCTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGCTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGATATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTGGATCATCTAAACGACGATGTCGACGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGATCGAGAAGTAAATCAGACACTTGTTCTTTTTCCCACCTCTTCCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGTTTAAACATTCTTTAATTGTTGGTGTACTTTTCTTTTTCTCTTCTTTTTGATCTTGTGGTTTTATAAGGTTGTAATAAGTACAAAGCTTTGCTTCTATTCTTTCAAAGAAAAAGCGTATATCCCCTCATCTTGTTGAAGATAGTTAGCAGCCATGAATTTAGACAATAAGAGCTGAACATTTAGTAGTTGTCACCGTTTGCTTTTCTTTTCTTTTCTTTTTCATGGGATTGACTAAAATGGAAGATAAAGCTACAATACACGAGAGAAAAGGGAAGATGTAAAAGAAAACTCAAATGGAGCTTTTACGTACATATATATTTCCATGATTCAGACAGGACAGCCTATACAGCAAAATTACATTAATTCCATAATCAATAAAAAAGAGATGTGAAAGTTCCATAGTACCCCTCCAAAGAAATAAAACCTCATGAATCCTGCCATGTCGGCATTTTGTACCTTATACCTCCAAAATTAATGAAACTCATGGGTTTCTTTTGAATCTTGCCTCCGGACCCGACCACCTATGACCTTTTTTTTTTAAGAAAAAA

Coding sequence (CDS)

ATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGCAGATCCTGAATTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATGCTGGGCCTGAAGCCAAACAAACTCTTAATGGTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAACTCCATGTCCTCTAACCCTTTTGATGATGATGATGATGGTTTTGTTGGAAAAAGGACTGCAACTTCTTCAGCCTCTAAAGACAGGTTCAAAAATGATTTTCGCGACTCGGGAGGACTTGAAAACCAGAGTGTGCAGGAATTGGAAAACTATGCTGCGTATAAAGCTGAGGAGACCACAAAATCTGTTAATAACTGCTTAAAGATTGCTGAGGACATAAGGGAAGATGCTACCAGAACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGGTGAGAAGCTTCTGAATAATCTTGGAGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGGCTGTCCAGATAATTCCTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGCTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGATATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTGGATCATCTAAACGACGATGTCGACGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGATCGAGAAGTAA

Protein sequence

MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSMSSNPFDDDDDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLGAEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRARRLIEK

Homology

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BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 6.4e-82
Identity = 175/305 (57.38%), Postives = 201/305 (65.90%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MF F KSP                 G N   +++      T+   RRTSSEP+L+ P+  
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITPD-- 60

Query: 61  SSNPFDDDDDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKSV 120
               FDDD                D++KN F DSGGL++Q+ +ELE YA YKAEETTK V
Sbjct: 61  ----FDDD----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGV 120

Query: 121 NNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 180
           NNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKPW
Sbjct: 121 NNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPW 180

Query: 181 KPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG------------------------ 240
           KPKKT+ ITGP+IT   PD  S ++EN+KE+REKLG                        
Sbjct: 181 KPKKTKNITGPMIT---PDKPSKKSENHKEEREKLGLGAKGRSSSQPALDQPTNALQKVE 240

Query: 241 AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRAR 282
            EK KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR
Sbjct: 241 QEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRAR 263

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BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.4e-76
Identity = 174/311 (55.95%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MF   KSPA + K NS D +    S  NPFDSD   + K TLN ++RT+SEP L    + 
Sbjct: 1   MFGLRKSPANLPKHNSVDLK---SSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SSNPFDDD----DDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEET 120
            +NPF  +     D    K++  S+ SK ++KN+FRDSGG+ENQSVQELE YA YKAEET
Sbjct: 61  MTNPFGGERVQKGDSSSSKQSLFSN-SKYQYKNNFRDSGGIENQSVQELEGYAVYKAEET 120

Query: 121 TKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 180
           TKSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMF
Sbjct: 121 TKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMF 180

Query: 181 SKPWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG-------------------- 240
           SK WKPKKTR I GP++T    D+S  R  N+ E+REKLG                    
Sbjct: 181 SKTWKPKKTRPINGPVVTR---DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESAD 240

Query: 241 ------AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKG 282
                  EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL+DDVDELN RV+ 
Sbjct: 241 AYQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQ 300

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BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.5e-54
Identity = 124/244 (50.82%), Postives = 161/244 (65.98%), Query Frame = 0


Query: 59  SMSSNPFDDDDDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 118
           S S NPFDDDDD             + RF +  + SGG ENQ+VQELE+YA Y +EETTK
Sbjct: 20  SFSLNPFDDDDD---------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEETTK 79

Query: 119 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 178
           +V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+FS+
Sbjct: 80  TVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSR 139

Query: 179 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------A 238
            WKPKK+R ITGP+IT G   +S  R   + + REKLG                      
Sbjct: 140 TWKPKKSRSITGPVITKG---DSPKRKVIDLKTREKLGLNPSLKPKSKTLPEAVDAYQKT 199

Query: 239 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRARR 282
           +  KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQRAR 
Sbjct: 200 QIAKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRARY 251

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BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot
Match: P36977 (Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 58/191 (30.37%), Postives = 99/191 (51.83%), Query Frame = 0


Query: 102 VQELENYAAYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKD 161
           + +++  A   A+E+ +S    L++ E+ ++   RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LSDMQQRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 162 LSKGEKLLNNLG---GMFSKPW-KPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG- 221
           +   EK LN+LG   G+ S P  K K            G   +   R  + +EQ    G 
Sbjct: 71  MKDAEKNLNDLGKFCGLCSCPCNKMKSGGSKAWGNNQDGVVASQPARVVDEREQMAISGG 130

Query: 222 --------AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRV 280
                   A + + D+ L  +  I+G+L+ MA+DMG+E+D QN+ +D + +  D   +R+
Sbjct: 131 FIRRVTDDARENEMDENLEQVGGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRI 190

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BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 57/193 (29.53%), Postives = 101/193 (52.33%), Query Frame = 0


Query: 102 VQELENYAAYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKD 161
           ++E++  A   A+E+ +S    L++ E+ ++   RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 162 LSKGEKLLNNLG---GMFSKPWKPKKTREITGPL---ITAGCPDNSSGRTENNKEQREKL 221
           + + EK L +LG   G+   P    K+ +           G   +   R  + +EQ    
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAIS 130

Query: 222 G---------AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNS 280
           G         A + + D+ L  +S I+G+L+ MA+DMG+E+D QN+ +D + +  D   +
Sbjct: 131 GGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKT 190

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BLAST of Lsi04G003720 vs. ExPASy TrEMBL
Match: A0A6J1J9F6 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111482502 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.1e-127
Identity = 258/308 (83.77%), Postives = 269/308 (87.34%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS D ELS GSGTNPFDSD+GPEAKQTLN ARRTSSEPVLVIPNS+
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SSNPF-DDDDDGFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
           S+N F DDDDDGFVGKR TATS+ASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSGRTENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
              A+KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

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BLAST of Lsi04G003720 vs. ExPASy TrEMBL
Match: A0A6J1H000 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111458786 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.5e-126
Identity = 255/308 (82.79%), Postives = 269/308 (87.34%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS D ELS GSGTNPFDSD+GPEAKQTLN ARRTSSEPVL+IPNS+
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSI 60

Query: 61  SSNPF-DDDDDGFVGK-RTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
           S+N F DDDDDGFVGK RTATS+ASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSGRTENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
              A+KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEAQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

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BLAST of Lsi04G003720 vs. ExPASy TrEMBL
Match: A0A0A0KQT5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606590 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.5e-126
Identity = 253/308 (82.14%), Postives = 265/308 (86.04%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS DPELSTGSGTNPFDSD GP+AKQTLN ARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60

Query: 61  SSNPFDDDDD-GFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
            +NPFDDDDD GFVG++ TATSS SKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  KANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSG+TENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
               EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

[Close]

BLAST of Lsi04G003720 vs. ExPASy TrEMBL
Match: A0A1S3BF13 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103489173 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.5e-126
Identity = 253/307 (82.41%), Postives = 265/307 (86.32%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS DPELSTGSGTNPFDSD GP+AKQTLN ARRTSSEPVL IPN  
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPN-- 60

Query: 61  SSNPFDDDDDGFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKS 120
            +NPF DDDDGFVG++ TATSS SKDR+KNDFRDSGGLENQSVQELENYA YKAEETTKS
Sbjct: 61  -ANPF-DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG----------------------- 240
           WKPKKT+EITGPLITA   D+SSG+TENNK+QREKLG                       
Sbjct: 181 WKPKKTKEITGPLITA---DHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQK 240

Query: 241 --AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQR 282
              EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQR
Sbjct: 241 VEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 300

[Close]

BLAST of Lsi04G003720 vs. ExPASy TrEMBL
Match: A0A6J1G0W1 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111449670 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 246/319 (77.12%), Postives = 258/319 (80.88%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSP KV+KQNS D  L+ GSGTNPFDSD  PE  QTLN ARRTSSEP LVIPNS+
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGLA-GSGTNPFDSDTEPETNQTLNPARRTSSEPALVIPNSI 60

Query: 61  SSNPF--DDDDDGFVGKR-------------TATSSASKDRFKNDFRDSGGLENQSVQEL 120
           S+NPF  DDDDDGFVG+R              ATSSASK+ +KNDFRDSGG ENQSVQEL
Sbjct: 61  SANPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQEL 120

Query: 121 ENYAAYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYA YKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLGA-------- 240
           EKLLNNLGGMFSKPWKPKKT+EITGPLITA   DNSSGRTENNKE+REKLG         
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITA---DNSSGRTENNKEEREKLGLSTGKKQSA 240

Query: 241 -----------------EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDD 280
                            EKEKQDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHL+DD
Sbjct: 241 TRTPPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDD 300

[Close]

BLAST of Lsi04G003720 vs. NCBI nr
Match: XP_038891847.1 (putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 putative SNAP25 homologous protein SNAP30 [Benincasa hispida])

HSP 1 Score: 486.5 bits (1251), Expect = 1.6e-133
Identity = 262/307 (85.34%), Postives = 272/307 (88.60%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MF FMKSPAKVTKQNS DPELSTGSGTNPFDSD GP+AKQTLN ARRTSSEPVL++PNSM
Sbjct: 1   MFGFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLIMPNSM 60

Query: 61  SSNPFDDDDDGFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKS 120
           SSNPFDDDD+GFVG+R TATSSASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTKS
Sbjct: 61  SSNPFDDDDEGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG----------------------- 240
           WKPKKTREITGPLITA   DNSSGRTENNKEQREKLG                       
Sbjct: 181 WKPKKTREITGPLITA---DNSSGRTENNKEQREKLGVSTGKKQSATQTPPSEQSGAMQK 240

Query: 241 --AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQR 282
             AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQR
Sbjct: 241 VEAEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 300

[Close]

BLAST of Lsi04G003720 vs. NCBI nr
Match: XP_022984084.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 466.1 bits (1198), Expect = 2.2e-127
Identity = 258/308 (83.77%), Postives = 269/308 (87.34%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS D ELS GSGTNPFDSD+GPEAKQTLN ARRTSSEPVLVIPNS+
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SSNPF-DDDDDGFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
           S+N F DDDDDGFVGKR TATS+ASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSGRTENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
              A+KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

[Close]

BLAST of Lsi04G003720 vs. NCBI nr
Match: XP_022957363.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 putative SNAP25-likeous protein SNAP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462.2 bits (1188), Expect = 3.1e-126
Identity = 255/308 (82.79%), Postives = 269/308 (87.34%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS D ELS GSGTNPFDSD+GPEAKQTLN ARRTSSEPVL+IPNS+
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSI 60

Query: 61  SSNPF-DDDDDGFVGK-RTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
           S+N F DDDDDGFVGK RTATS+ASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSGRTENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
              A+KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEAQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

[Close]

BLAST of Lsi04G003720 vs. NCBI nr
Match: KAG6601467.1 (putative SNAP25-likeous protein SNAP30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 460.7 bits (1184), Expect = 9.1e-126
Identity = 255/308 (82.79%), Postives = 268/308 (87.01%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS D ELS GSGTNPFDSD+GPEAKQTLN ARRTSSEPVLVIPNS+
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SSNPF-DDDDDGFVGK-RTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
           S+N F DDDDDGFVGK R ATS+ASKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRAATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSGRTENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
              A+KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEAQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

[Close]

BLAST of Lsi04G003720 vs. NCBI nr
Match: XP_004135151.1 (putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_031740914.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >KGN51968.1 hypothetical protein Csa_009347 [Cucumis sativus])

HSP 1 Score: 459.9 bits (1182), Expect = 1.6e-125
Identity = 253/308 (82.14%), Postives = 265/308 (86.04%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MFSFMKSPAKVTKQNS DPELSTGSGTNPFDSD GP+AKQTLN ARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60

Query: 61  SSNPFDDDDD-GFVGKR-TATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 120
            +NPFDDDDD GFVG++ TATSS SKDR+KNDFRDSGGLENQSVQELENYA YKAEETTK
Sbjct: 61  KANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------- 240
           PWKPKKT+EITGPLITA   D+SSG+TENNKEQREKLG                      
Sbjct: 181 PWKPKKTKEITGPLITA---DHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQ 240

Query: 241 ---AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQ 282
               EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHL+DDVDELNSRVKGANQ
Sbjct: 241 KVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQ 300

[Close]

BLAST of Lsi04G003720 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 305.4 bits (781), Expect = 4.6e-83
Identity = 175/305 (57.38%), Postives = 201/305 (65.90%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MF F KSP                 G N   +++      T+   RRTSSEP+L+ P+  
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITPD-- 60

Query: 61  SSNPFDDDDDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKSV 120
               FDDD                D++KN F DSGGL++Q+ +ELE YA YKAEETTK V
Sbjct: 61  ----FDDD----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGV 120

Query: 121 NNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 180
           NNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKPW
Sbjct: 121 NNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPW 180

Query: 181 KPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG------------------------ 240
           KPKKT+ ITGP+IT   PD  S ++EN+KE+REKLG                        
Sbjct: 181 KPKKTKNITGPMIT---PDKPSKKSENHKEEREKLGLGAKGRSSSQPALDQPTNALQKVE 240

Query: 241 AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRAR 282
            EK KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR
Sbjct: 241 QEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRAR 263

[Close]

BLAST of Lsi04G003720 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 287.7 bits (735), Expect = 9.9e-78
Identity = 174/311 (55.95%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSADPELSTGSGTNPFDSDAGPEAKQTLNGARRTSSEPVLVIPNSM 60
           MF   KSPA + K NS D +    S  NPFDSD   + K TLN ++RT+SEP L    + 
Sbjct: 1   MFGLRKSPANLPKHNSVDLK---SSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SSNPFDDD----DDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEET 120
            +NPF  +     D    K++  S+ SK ++KN+FRDSGG+ENQSVQELE YA YKAEET
Sbjct: 61  MTNPFGGERVQKGDSSSSKQSLFSN-SKYQYKNNFRDSGGIENQSVQELEGYAVYKAEET 120

Query: 121 TKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 180
           TKSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMF
Sbjct: 121 TKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMF 180

Query: 181 SKPWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG-------------------- 240
           SK WKPKKTR I GP++T    D+S  R  N+ E+REKLG                    
Sbjct: 181 SKTWKPKKTRPINGPVVTR---DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESAD 240

Query: 241 ------AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKG 282
                  EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL+DDVDELN RV+ 
Sbjct: 241 AYQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQ 300

[Close]

BLAST of Lsi04G003720 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-55
Identity = 124/244 (50.82%), Postives = 161/244 (65.98%), Query Frame = 0


Query: 59  SMSSNPFDDDDDGFVGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTK 118
           S S NPFDDDDD             + RF +  + SGG ENQ+VQELE+YA Y +EETTK
Sbjct: 20  SFSLNPFDDDDD---------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEETTK 79

Query: 119 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 178
           +V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+FS+
Sbjct: 80  TVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSR 139

Query: 179 PWKPKKTREITGPLITAGCPDNSSGRTENNKEQREKLG---------------------A 238
            WKPKK+R ITGP+IT G   +S  R   + + REKLG                      
Sbjct: 140 TWKPKKSRSITGPVITKG---DSPKRKVIDLKTREKLGLNPSLKPKSKTLPEAVDAYQKT 199

Query: 239 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRARR 282
           +  KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQRAR 
Sbjct: 200 QIAKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRARY 251

[Close]

BLAST of Lsi04G003720 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 42.0 bits (97), Expect = 9.4e-04
Identity = 63/231 (27.27%), Postives = 94/231 (40.69%), Query Frame = 0


Query: 73  VGKRTATSSASKDRFKNDFRDSGGLENQSVQELENYAAYKAEETTKSVNNCLKIAEDIRE 132
           V K+   + +  D F    R  G  E Q    LE     KAE  TK  N    +A +   
Sbjct: 22  VDKQREANISGDDAFA---RLYGAFETQIETALE-----KAELVTKEKNRAAAVAMNAEI 81

Query: 133 DATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK----------- 192
             T+      +  E++ +  R+A    K L+  E    N   + + P +           
Sbjct: 82  RRTKA-----RLSEEVPKLQRLAVKRVKGLTTEELAARN-DLVLALPARIEAIPDGTAGG 141

Query: 193 PKKTREITGPLITAGCPD---NSSGRTENNKEQ--------REKLGAEKEKQDDALSDLS 252
           PK T   T P  T   PD   +S GR +++  Q        R++    K KQ+  L  +S
Sbjct: 142 PKSTSAWT-PSSTTSRPDIKFDSDGRFDDDYFQESNESSQFRQEYEMRKIKQEQGLDMIS 201

Query: 253 NILGDLKSMAVDMGSELDRQNKALDHLNDDVDELNSRVKGANQRARRLIEK 282
             L  LK+MA DM  ELDRQ   +D ++  VD   S +K  N R +  + +
Sbjct: 202 EGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATSDLKNTNVRLKDTVNQ 237

The following BLAST results are available for this feature:

BLAST of Lsi04G003720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMG8	6.4e-82	57.38	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9S7P9	1.4e-76	55.95	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9SD96	2.5e-54	50.82	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
P36977	1.2e-14	30.37	Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=...	[more]
Q17QQ3	1.2e-14	29.53	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]

BLAST of Lsi04G003720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J9F6	1.1e-127	83.77	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1H000	1.5e-126	82.79	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0KQT5	7.5e-126	82.14	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A1S3BF13	7.5e-126	82.41	putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10348917...	[more]
A0A6J1G0W1	1.1e-119	77.12	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Lsi04G003720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891847.1	1.6e-133	85.34	putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 pu...	[more]
XP_022984084.1	2.2e-127	83.77	putative SNAP25 homologous protein SNAP30 [Cucurbita maxima]	[more]
XP_022957363.1	3.1e-126	82.79	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 put...	[more]
KAG6601467.1	9.1e-126	82.79	putative SNAP25-likeous protein SNAP30, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_004135151.1	1.6e-125	82.14	putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 puta...	[more]

BLAST of Lsi04G003720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13890.1	4.6e-83	57.38	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G61210.1	9.9e-78	55.95	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT5G07880.1	1.8e-55	50.82	synaptosomal-associated protein SNAP25-like 29	[more]
AT3G09740.1	9.4e-04	27.27	syntaxin of plants 71	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 247..281
None	No IPR available	COILS	Coil	Coil	coord: 124..144
None	No IPR available	GENE3D	1.20.5.110		coord: 96..174 e-value: 3.7E-22 score: 80.1
None	No IPR available	GENE3D	1.20.5.110		coord: 199..279 e-value: 4.9E-23 score: 83.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..65
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..96
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 218..281
None	No IPR available	PANTHER	PTHR19305:SF25	SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATED	coord: 1..218
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 1..218
None	No IPR available	PANTHER	PTHR19305:SF25	SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATED	coord: 218..281
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 219..277 e-value: 6.54638E-17 score: 71.0504
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 207..278
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 100..171
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 211..278 e-value: 7.3E-12 score: 55.4 coord: 105..172 e-value: 0.047 score: 22.8
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 216..278 score: 17.685278
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 107..171 e-value: 2.23184E-21 score: 83.3915

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G003720.1	Lsi04G003720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity