Lsi04G003440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G003440
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description1-phosphatidylinositol 4-kinase
Locationchr04: 3427395 .. 3429332 (-)
RNA-Seq ExpressionLsi04G003440
SyntenyLsi04G003440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGAATTACCGCTCGAGAAATGGAATGGACGAAAAACAGCCTGTTGGAAGGAGGCAAGTTTTTGTGCAGACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGTAATGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCGCTGACATTTGGGGATGTGATACTAAAGAATGATCTAAGTGCCATTCGGAATGATTCTCCTCTTCTCCTCACGAGAAATCTCTTGCACAGAAGCTCATCGACTCCATGTCTCTCACCCACTGGGAAGGATCTCCGGCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATTCAGATCGTTTTTCTGGCACAAAACGGTTGGTCAAAGAGATTGTGAAAGCTATTAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGAGGCGCATACTATTTTAGGAATGGGAAAGGTGAAAGTGTGGCAATTGTGAAGCCAACCGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTCGGACAACCTGGATTTAAACGTTCTGTGCGTGTTGGTGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAGCATTTTGCAAATGTGCCTCCGACAGCCTTGGTGAAGATCACTCACTCAATCTTCAATGTGAATGATTGGGTGAATGGTAACAAGCCTCAGGGTCACAAGAAGAAGTCGGTTAGTAAAATTGCTTCCTTCCAGCAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGCTTCCCCGTCGCTGCTGTCCATCGTATAGGTATTCTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGATGGAATAGGAAGGTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAGACTTTAGAGGATCCATACTTTGAGTGGATTCACTGGCCACAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATCAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGCCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAAATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAGGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATCATAGCAGAAAGGGAGTTCTGTTCTCCCACTTCTGATTGGAGCAATGAGGAATTCCAGTTTGATTTAGACTGTGAGGATGGAGAATTAGATTATAACTCAGGTATGGCAGCGGACCAGTGTTTGGGCTGGTCACATTACCAGTTTGGTGGACTTGGAAGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAAGAGGAGGGAGAAGATAGTGGAGGGGAGGAGCAAGGAGAAACAGCTACCTTTCTTCCTTCTGAACCCATTTCTGTTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCGATAAGAATCAGAAAACGCAAAAGTACCAGGTCACAAAACCTGAATATGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAATGAGCAGTTTCCTCCAAGTGTCAGCTTTGTTAAGCTCGGGGATATGAACGAGGAAGAGTGGTCACTTTTTCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCATTTGCCAAACGCAAAGCTGCGGCTATGGGGCAGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

mRNA sequence

ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGAATTACCGCTCGAGAAATGGAATGGACGAAAAACAGCCTGTTGGAAGGAGGCAAGTTTTTGTGCAGACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGTAATGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCGCTGACATTTGGGGATGTGATACTAAAGAATGATCTAAGTGCCATTCGGAATGATTCTCCTCTTCTCCTCACGAGAAATCTCTTGCACAGAAGCTCATCGACTCCATGTCTCTCACCCACTGGGAAGGATCTCCGGCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATTCAGATCGTTTTTCTGGCACAAAACGGTTGGTCAAAGAGATTGTGAAAGCTATTAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGAGGCGCATACTATTTTAGGAATGGGAAAGGTGAAAGTGTGGCAATTGTGAAGCCAACCGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTCGGACAACCTGGATTTAAACGTTCTGTGCGTGTTGGTGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAGCATTTTGCAAATGTGCCTCCGACAGCCTTGGTGAAGATCACTCACTCAATCTTCAATGTGAATGATTGGGTGAATGGTAACAAGCCTCAGGGTCACAAGAAGAAGTCGGTTAGTAAAATTGCTTCCTTCCAGCAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGCTTCCCCGTCGCTGCTGTCCATCGTATAGGTATTCTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGATGGAATAGGAAGGTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAGACTTTAGAGGATCCATACTTTGAGTGGATTCACTGGCCACAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATCAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGCCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAAATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAGGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATCATAGCAGAAAGGGAGTTCTGTTCTCCCACTTCTGATTGGAGCAATGAGGAATTCCAGTTTGATTTAGACTGTGAGGATGGAGAATTAGATTATAACTCAGGTATGGCAGCGGACCAGTGTTTGGGCTGGTCACATTACCAGTTTGGTGGACTTGGAAGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAAGAGGAGGGAGAAGATAGTGGAGGGGAGGAGCAAGGAGAAACAGCTACCTTTCTTCCTTCTGAACCCATTTCTGTTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCGATAAGAATCAGAAAACGCAAAAGTACCAGGTCACAAAACCTGAATATGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAATGAGCAGTTTCCTCCAAGTGTCAGCTTTGTTAAGCTCGGGGATATGAACGAGGAAGAGTGGTCACTTTTTCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCATTTGCCAAACGCAAAGCTGCGGCTATGGGGCAGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

Coding sequence (CDS)

ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGAATTACCGCTCGAGAAATGGAATGGACGAAAAACAGCCTGTTGGAAGGAGGCAAGTTTTTGTGCAGACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGTAATGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCGCTGACATTTGGGGATGTGATACTAAAGAATGATCTAAGTGCCATTCGGAATGATTCTCCTCTTCTCCTCACGAGAAATCTCTTGCACAGAAGCTCATCGACTCCATGTCTCTCACCCACTGGGAAGGATCTCCGGCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATTCAGATCGTTTTTCTGGCACAAAACGGTTGGTCAAAGAGATTGTGAAAGCTATTAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGAGGCGCATACTATTTTAGGAATGGGAAAGGTGAAAGTGTGGCAATTGTGAAGCCAACCGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTCGGACAACCTGGATTTAAACGTTCTGTGCGTGTTGGTGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAGCATTTTGCAAATGTGCCTCCGACAGCCTTGGTGAAGATCACTCACTCAATCTTCAATGTGAATGATTGGGTGAATGGTAACAAGCCTCAGGGTCACAAGAAGAAGTCGGTTAGTAAAATTGCTTCCTTCCAGCAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGCTTCCCCGTCGCTGCTGTCCATCGTATAGGTATTCTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGATGGAATAGGAAGGTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAGACTTTAGAGGATCCATACTTTGAGTGGATTCACTGGCCACAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATCAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGCCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAAATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAGGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATCATAGCAGAAAGGGAGTTCTGTTCTCCCACTTCTGATTGGAGCAATGAGGAATTCCAGTTTGATTTAGACTGTGAGGATGGAGAATTAGATTATAACTCAGGTATGGCAGCGGACCAGTGTTTGGGCTGGTCACATTACCAGTTTGGTGGACTTGGAAGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAAGAGGAGGGAGAAGATAGTGGAGGGGAGGAGCAAGGAGAAACAGCTACCTTTCTTCCTTCTGAACCCATTTCTGTTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCGATAAGAATCAGAAAACGCAAAAGTACCAGGTCACAAAACCTGAATATGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAATGAGCAGTTTCCTCCAAGTGTCAGCTTTGTTAAGCTCGGGGATATGAACGAGGAAGAGTGGTCACTTTTTCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCATTTGCCAAACGCAAAGCTGCGGCTATGGGGCAGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

Protein sequence

MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRNDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF
Homology
BLAST of Lsi04G003440 vs. ExPASy Swiss-Prot
Match: Q9SI52 (Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 PE=1 SV=2)

HSP 1 Score: 877.1 bits (2265), Expect = 1.2e-253
Identity = 459/666 (68.92%), Postives = 529/666 (79.43%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+   +       K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRNDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDR+DN HTVKRRLQ+ALN PTEESSLT+GD++L NDLSA+RNDSPLLL RN +HRSS
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRSS 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGA 180
           STPCLSPTG+DL+ +D+SGPIEILGHSD FS  K +VK+IVKA+K+G++P+PVHSGLGGA
Sbjct: 121 STPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGGA 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
           YYFRN +GESVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLD
Sbjct: 181 YYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP SD   E EFQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGET 540
           DC+D E  Y+S +        +   F      +GR+ L KLEESI+EE ED   EE+ +T
Sbjct: 481 DCDDLESVYSSKIQL------TDDYFTKNPFSNGRSSLGKLEESIKEEEEDE-EEEEDKT 540

Query: 541 ATFLP--------------SEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITN 600
              +P               +    +SKLS S+KNT L D  +K  K  +T+   G   N
Sbjct: 541 ENTVPMIIMKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKSEN 600

Query: 601 TSAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRL 646
           TS+GH+SANEQ P S SFVK+ DM E+EW LFL++F ELL PAFAKRK A +   +RQRL
Sbjct: 601 TSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRL 650

BLAST of Lsi04G003440 vs. ExPASy Swiss-Prot
Match: Q9C671 (Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 PE=1 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 1.1e-249
Identity = 447/649 (68.88%), Postives = 523/649 (80.59%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSP++TQMAVA +KSPL+  +R  N +  K PVGRR+VFVQT+TGCVLG+ELDR+
Sbjct: 1   MSRKLDSPIKTQMAVALVKSPLNGEFREFNKVGMKTPVGRRRVFVQTETGCVLGLELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKR+LQ+ALN P EESSLTFGD++LKNDL+A+R+DSPLLLTRN  HRSSSTPCLS
Sbjct: 61  DNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPCLS 120

Query: 121 PTGKDL-RLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRN 180
           P   DL + RD+S PIEILG+S  FS  +++ K+I KA+K GIDP+ V+SGLGGAYYF+N
Sbjct: 121 PMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYFKN 180

Query: 181 GKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFA 240
            +GESVAIVKPTDEEP+APNNPKGFVGKALGQPG KRSVRVGETG+REVAAYLLD EHFA
Sbjct: 181 SRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEHFA 240

Query: 241 NVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPV 300
           NVPPTALVKITHSIFNVND V  +KP   +K  VSKIAS QQFIPHD+DAS+HGTS+FPV
Sbjct: 241 NVPPTALVKITHSIFNVNDGVKASKPM--EKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 301 AAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWI 360
           +AVHRIGILDIRI NTDRH+GNLLV+KLDG G FGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 361 HWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLC 420
           HWPQASIPFS+DELKYI NLDP+ DCEMLR ELPM+REA LRVLVLCTIFLKEAAA+GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 421 LAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDLDCEDG 480
           LAEIGEMM+RE R G+EEPSE+E+VCLEA  +I E++  SP SD  N+ EFQFD+DCE+ 
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEE- 480

Query: 481 ELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIE--EEGEDSGGEEQGETATF 540
             D    +A             GL   + R  LSK+EE+ E  EE E+   EE+      
Sbjct: 481 VTDCTKKLALPL----------GLTFGNARGQLSKVEETTEDGEEEEEEDREEEENDRAD 540

Query: 541 LPSEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVS 600
           L   P     KLSMSLK+T+LG+K+QK QK+   + E  Y    S+ HRSA+EQ P S S
Sbjct: 541 LEKMP---TIKLSMSLKSTLLGEKSQKYQKHPGARVESAY---ASSAHRSADEQIPSSTS 600

Query: 601 FVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           FVKL DM+EEEW++FL+K+ ELL PA  KRK+  +GQ+QRQRLGTSCQF
Sbjct: 601 FVKLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of Lsi04G003440 vs. ExPASy Swiss-Prot
Match: Q8W4R8 (Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 3.4e-235
Identity = 422/645 (65.43%), Postives = 497/645 (77.05%), Query Frame = 0

Query: 13  MAVAALKSPLSRNYRSRNGMD---------EKQPVGRRQVFVQTDTGCVLGMELDRNDNA 72
           MA+A  K+PL   +     M+         ++Q  GRR+VFVQTDTGCVLG+ELDRNDN 
Sbjct: 1   MAMAVFKAPLKGEFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNV 60

Query: 73  HTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTG 132
           HTVK+RLQ+A N PTEESSLTFGD++LKNDLSA+RNDSPLLL RNL+HRSSSTPCLSPTG
Sbjct: 61  HTVKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTG 120

Query: 133 KDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNGKGE 192
            DL+ +D+SGPIEIL HS  F   K+   +IVKA+K+G++PIPV+ GLGGAYYFR+ KG+
Sbjct: 121 NDLQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQ 180

Query: 193 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPP 252
           SVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLDY+HFANVPP
Sbjct: 181 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPP 240

Query: 253 TALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVAAVH 312
           TALVKITHS+FNVND ++GNK +  KK   SKIASFQ+F+PHDFDASDHGTSSFPVA+VH
Sbjct: 241 TALVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVH 300

Query: 313 RIGILDIRIFNTDRHAGNLLVRKLD--GIGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 372
           RIGILDIRI NTDRH GNLLV+KLD  G+GRFGQVELIPIDHGLCLPETLEDPYFEWIHW
Sbjct: 301 RIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 360

Query: 373 PQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLA 432
           PQASIPFS++EL YI++LDP+KDCEMLR ELPMIREACLRVLVLCT+FLKEAA  GLCLA
Sbjct: 361 PQASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLA 420

Query: 433 EIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDLDCEDGEL 492
           EIGEMM+REFR+GEEEPSELE++C+EA+++  E++  SP SD   E EFQFD+D  + + 
Sbjct: 421 EIGEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDS 480

Query: 493 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 552
            Y S    D+    + +        +GR+ L +L+ESI EE ED   EE+ +    L   
Sbjct: 481 VYGSETETDEFFAKNPFS-------NGRSSLGELKESIAEEEEDD--EEEAKLTLSL--- 540

Query: 553 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 612
                SKLS S+KN +    N     Y +  P+          H+SAN Q P SV+FVKL
Sbjct: 541 -----SKLSTSMKNNL---SNTMGSGY-LKPPKDNQTDKALVSHKSANVQLPLSVNFVKL 600

Query: 613 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
            DM E EW +FL++F ELL  AFA+RK   M  R  QRLGTSC+F
Sbjct: 601 ADMKEVEWVVFLERFQELLYSAFAERK--TMTLRNTQRLGTSCKF 622

BLAST of Lsi04G003440 vs. ExPASy Swiss-Prot
Match: O22199 (Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.5e-102
Identity = 229/544 (42.10%), Postives = 306/544 (56.25%), Query Frame = 0

Query: 110 LHRSSSTPCLS---PTGKDLRLRDQSGP-IEILGHSDRFSGTKRLVKEIVKAIKLGIDPI 169
           +HRS S+PC S       +  L+  + P IEILG   R    + LV E+  A+  G  P+
Sbjct: 67  IHRSVSSPCFSIAASANMEEDLKATTAPRIEILG-GQRVPTVRALVAEVTMAMVSGAQPL 126

Query: 170 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 229
            + SG+GGAY  + GKG ++A+ KP DEEP A NNPK      LGQPG K S+ VGETG 
Sbjct: 127 LLPSGMGGAYLLQTGKGHNIAVAKPVDEEPLAFNNPKKSGNLMLGQPGMKHSIPVGETGI 186

Query: 230 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPH 289
           RE+AAYLLDY+ F+ VPPTALV I+H  F+V+D  + +           K+AS Q+F+ H
Sbjct: 187 RELAAYLLDYQGFSGVPPTALVSISHVPFHVSDAFSFS-------SMPYKVASLQRFVGH 246

Query: 290 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLD---GIGRFGQVELIPID 349
           DFDA + G  SF   +VHRIGILD+R+ N DRHAGN+LV++ D      R G  EL+PID
Sbjct: 247 DFDAGELGPGSFTATSVHRIGILDVRLLNLDRHAGNMLVKRCDKKEAYNRLGTAELVPID 306

Query: 350 HGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRV 409
           HGLCLPE L+DPYFEW++WPQA +PFSD EL YI NLDP KD E+LR EL  + E+ +RV
Sbjct: 307 HGLCLPECLDDPYFEWLNWPQALVPFSDTELDYISNLDPFKDAELLRTELHSLPESAIRV 366

Query: 410 LVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTS 469
           LV+CT+FLK+AAA+GLCLAEIGE M+R+F  GEE  S LE +C +A+  +  +   S  S
Sbjct: 367 LVVCTVFLKQAAAAGLCLAEIGEKMTRDFSKGEESFSLLETLCTKAKASVFGK--TSEDS 426

Query: 470 DWSNEEFQFDLDCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEG 529
           D+S+E  + + + + G   ++ G                          +  E  I E  
Sbjct: 427 DYSHEGNEVNTELQCGMFKFDGGD-------------------------TPCEAEISEVF 486

Query: 530 EDSGGE--EQGETATFLPSEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNT 589
             S      +G  A  +P++   V + +S S    I   +    +K +  K E   + + 
Sbjct: 487 HVSKPPLVPRGPRANTIPND---VTASMSSSQNQRITHQEKSAKEKKRGGKQERCTVRSK 546

Query: 590 SAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLG 645
           S    S N      VSFV   DM   EW  FL  F  LL+ A +K            RLG
Sbjct: 547 SP-PISPNHDESKGVSFV---DMTTVEWDTFLQSFQTLLQDALSKGST--------PRLG 560

BLAST of Lsi04G003440 vs. ExPASy Swiss-Prot
Match: Q0WMZ6 (Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 PE=2 SV=2)

HSP 1 Score: 369.0 bits (946), Expect = 1.1e-100
Identity = 199/390 (51.03%), Postives = 261/390 (66.92%), Query Frame = 0

Query: 106 TRNLLHRSSSTPCLSPTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPI 165
           + +L +RS STPC S +G +L     +  IEILG   R    + LV E+  AI  G  P+
Sbjct: 48  SHSLNNRSVSTPCFSISGSNLD-GSATPHIEILG-GQRVPTVRSLVAEVTIAIVSGAQPL 107

Query: 166 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 225
            + SGLGGAY  +  KG ++A+ KP DEEP A NNPKG  G  LGQPG KRS+RVGE+G 
Sbjct: 108 LLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNPKGSGGLTLGQPGMKRSIRVGESGI 167

Query: 226 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPH 285
           RE+AAYLLD++ F++VPPTALV+I+H  F+       ++   H   +  K+AS Q+F+ H
Sbjct: 168 RELAAYLLDHQGFSSVPPTALVRISHVPFH-------DRGSDH---AAYKVASLQRFVGH 227

Query: 286 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDG------IGRFGQVELI 345
           DFDA + G  SF V +VHRIGILD+R+ N DRHAGN+LV+K+            G  EL+
Sbjct: 228 DFDAGELGPGSFTVVSVHRIGILDVRVLNLDRHAGNMLVKKIHDQDETTCSNGVGAAELV 287

Query: 346 PIDHGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREAC 405
           PIDHGLCLPE L+DPYFEW++WPQAS+PF+D EL+YI NLDP KD E+LR EL  I+E+ 
Sbjct: 288 PIDHGLCLPECLDDPYFEWLNWPQASVPFTDIELQYISNLDPFKDAELLRTELDSIQESS 347

Query: 406 LRVLVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEAR--QIIAEREF 465
           LRVL++CTIFLKEAAA+GL LAEIGE M+R+   GEE  S LE++C +A+   +    + 
Sbjct: 348 LRVLIVCTIFLKEAAAAGLSLAEIGEKMTRDICRGEESSSVLEILCNKAKASAVSGSDDD 407

Query: 466 CSPTSDWSNEE-------FQFDLDCEDGEL 481
              +S+W+  E       FQFD + E  EL
Sbjct: 408 DDYSSEWNEVEAELECGIFQFDDEVECKEL 425

BLAST of Lsi04G003440 vs. ExPASy TrEMBL
Match: A0A1S3BFU2 (1-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103489203 PE=3 SV=1)

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 633/645 (98.14%), Postives = 638/645 (98.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+Y S+NG DEKQPVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQ HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREF SPTSDWSNEEFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DY+SGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETA FLPSE
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PISVVSKLSMSLKNTILGDKNQK QKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMNEEEWSLFLDKFPELLRPAFAKRK+A +GQRQRQRLGTSCQF
Sbjct: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Lsi04G003440 vs. ExPASy TrEMBL
Match: A0A5D3CB14 (1-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00300 PE=3 SV=1)

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 633/645 (98.14%), Postives = 638/645 (98.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+Y S+NG DEKQPVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQ HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREF SPTSDWSNEEFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DY+SGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETA FLPSE
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PISVVSKLSMSLKNTILGDKNQK QKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMNEEEWSLFLDKFPELLRPAFAKRK+A +GQRQRQRLGTSCQF
Sbjct: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Lsi04G003440 vs. ExPASy TrEMBL
Match: A0A0A0KWA5 (1-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G606910 PE=3 SV=1)

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 625/645 (96.90%), Postives = 636/645 (98.60%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+Y S+NG DEK PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGTKRLVKEIVKAIKLG+DPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTKRLVKEIVKAIKLGVDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNK Q HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKSQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGTFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREF SPTS+WSNEEFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSEWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DY+SGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETA+FLPSE
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETASFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           P+SVVSKLSMSLKNTILGDKNQK QKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PVSVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMNEEEWS+FLDKFPELLRPAFAKRK+A +GQRQRQRLGTSCQF
Sbjct: 601 GDMNEEEWSIFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Lsi04G003440 vs. ExPASy TrEMBL
Match: A0A6J1HTY7 (1-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111467446 PE=3 SV=1)

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 630/645 (97.67%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+YR+RNGM++K PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYRARNGMEDKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQ+ALNIPTEESSLTFGDVILKNDL+AIR+DSPLLLTRNL+HRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQIALNIPTEESSLTFGDVILKNDLTAIRSDSPLLLTRNLMHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGT+RLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTRRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKP  HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKP--HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFS+DELKYIENL+PIKDCEMLRMELPMIR+ACLRVLVLCTIFLKEAAA GLCL
Sbjct: 361 WPQASIPFSEDELKYIENLNPIKDCEMLRMELPMIRDACLRVLVLCTIFLKEAAACGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERE CSP  DW N+EFQFDLDCE+ EL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERELCSPVDDWGNDEFQFDLDCEEREL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DYNS +AADQCLGWSHYQFGGLG +HGRNPLSKLEESIEEEGEDS GEEQGETATF PSE
Sbjct: 481 DYNSSVAADQCLGWSHYQFGGLGGMHGRNPLSKLEESIEEEGEDSAGEEQGETATFPPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PIS VSKLSMSLKNTILGDKNQK QKYQV+KPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISAVSKLSMSLKNTILGDKNQKHQKYQVSKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMN+EEW+LFLDKFPE+LRPAFAKR++AA+GQRQRQRLGTSCQF
Sbjct: 601 GDMNDEEWTLFLDKFPEVLRPAFAKRQSAALGQRQRQRLGTSCQF 643

BLAST of Lsi04G003440 vs. ExPASy TrEMBL
Match: A0A6J1FZC8 (1-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111449302 PE=3 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 630/645 (97.67%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+YR+RNGM+EK PVGRRQVFVQTD+GCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYRARNGMEEKLPVGRRQVFVQTDSGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQ+ALNIPTEESSLTFGDVILKNDL+AIR+DSPLLLTRNL+HRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQIALNIPTEESSLTFGDVILKNDLTAIRSDSPLLLTRNLMHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGT+RLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTRRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKP  HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKP--HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFS+DELKYIENL+PIKDCEMLRMELPMIR+ACLRVLVLCTIFLKEAAA GLCL
Sbjct: 361 WPQASIPFSEDELKYIENLNPIKDCEMLRMELPMIRDACLRVLVLCTIFLKEAAACGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERE CSP  DW N+EFQFDLDCE+ EL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERELCSPVDDWGNDEFQFDLDCEEREL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DYNS +AADQCLGWSHYQFGGLG +HGRNPLSKLEESIEEEGEDS GEEQGETATF PSE
Sbjct: 481 DYNSSVAADQCLGWSHYQFGGLGGMHGRNPLSKLEESIEEEGEDSAGEEQGETATFPPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PIS VSKLSMSLKNTILGDKNQK QKYQV+KPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISAVSKLSMSLKNTILGDKNQKHQKYQVSKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMN+EEW+LFLDKFPE+LRPAFAKR++AA+GQRQRQRLGTSCQF
Sbjct: 601 GDMNDEEWTLFLDKFPEVLRPAFAKRQSAALGQRQRQRLGTSCQF 643

BLAST of Lsi04G003440 vs. NCBI nr
Match: XP_008446479.1 (PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >XP_008446480.1 PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >KAA0034489.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis melo var. makuwa] >TYK09043.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 633/645 (98.14%), Postives = 638/645 (98.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+Y S+NG DEKQPVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQ HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREF SPTSDWSNEEFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DY+SGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETA FLPSE
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PISVVSKLSMSLKNTILGDKNQK QKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMNEEEWSLFLDKFPELLRPAFAKRK+A +GQRQRQRLGTSCQF
Sbjct: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Lsi04G003440 vs. NCBI nr
Match: XP_004135137.1 (phosphatidylinositol 4-kinase gamma 5 [Cucumis sativus] >XP_004135138.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis sativus] >XP_031741067.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis sativus] >KGN52001.1 hypothetical protein Csa_008681 [Cucumis sativus])

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 625/645 (96.90%), Postives = 636/645 (98.60%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+Y S+NG DEK PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGTKRLVKEIVKAIKLG+DPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTKRLVKEIVKAIKLGVDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNK Q HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKSQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGTFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREF SPTS+WSNEEFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSEWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DY+SGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETA+FLPSE
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETASFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           P+SVVSKLSMSLKNTILGDKNQK QKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PVSVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMNEEEWS+FLDKFPELLRPAFAKRK+A +GQRQRQRLGTSCQF
Sbjct: 601 GDMNEEEWSIFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Lsi04G003440 vs. NCBI nr
Match: KAG6574027.1 (Phosphatidylinositol 4-kinase gamma 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013086.1 Phosphatidylinositol 4-kinase gamma 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 631/645 (97.83%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+YR+RNGM+EK PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYRARNGMEEKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQ+ALNIPTEESSLTFGDVILKNDL+AIR+DSPLLLTRNL+HRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQIALNIPTEESSLTFGDVILKNDLTAIRSDSPLLLTRNLMHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGT+RLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTRRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKP  HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKP--HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFS+DELKYIENL+PIKDCEMLRMELPMIR+ACLRVLVLCTIFLKEAAA GLCL
Sbjct: 361 WPQASIPFSEDELKYIENLNPIKDCEMLRMELPMIRDACLRVLVLCTIFLKEAAACGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERE CSP  DW N+EFQFDLDCE+ EL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERELCSPIDDWGNDEFQFDLDCEEREL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DYNS +AADQCLGWSHYQFGGLG +HGRNPLSKLEESIEEEGEDS GEEQG+TATFLPSE
Sbjct: 481 DYNSSVAADQCLGWSHYQFGGLGGMHGRNPLSKLEESIEEEGEDSAGEEQGDTATFLPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PIS V+KLSMSLKNTILGDKNQK QKYQV+KPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISAVTKLSMSLKNTILGDKNQKHQKYQVSKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMN+EEW+LFLDKFPE+LRPAFAKR++AA+GQRQRQRLGTSCQF
Sbjct: 601 GDMNDEEWTLFLDKFPEVLRPAFAKRQSAALGQRQRQRLGTSCQF 643

BLAST of Lsi04G003440 vs. NCBI nr
Match: XP_022968111.1 (phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022968112.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022968113.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 630/645 (97.67%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+YR+RNGM++K PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYRARNGMEDKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQ+ALNIPTEESSLTFGDVILKNDL+AIR+DSPLLLTRNL+HRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQIALNIPTEESSLTFGDVILKNDLTAIRSDSPLLLTRNLMHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGT+RLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTRRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKP  HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKP--HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFS+DELKYIENL+PIKDCEMLRMELPMIR+ACLRVLVLCTIFLKEAAA GLCL
Sbjct: 361 WPQASIPFSEDELKYIENLNPIKDCEMLRMELPMIRDACLRVLVLCTIFLKEAAACGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERE CSP  DW N+EFQFDLDCE+ EL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERELCSPVDDWGNDEFQFDLDCEEREL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DYNS +AADQCLGWSHYQFGGLG +HGRNPLSKLEESIEEEGEDS GEEQGETATF PSE
Sbjct: 481 DYNSSVAADQCLGWSHYQFGGLGGMHGRNPLSKLEESIEEEGEDSAGEEQGETATFPPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PIS VSKLSMSLKNTILGDKNQK QKYQV+KPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISAVSKLSMSLKNTILGDKNQKHQKYQVSKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMN+EEW+LFLDKFPE+LRPAFAKR++AA+GQRQRQRLGTSCQF
Sbjct: 601 GDMNDEEWTLFLDKFPEVLRPAFAKRQSAALGQRQRQRLGTSCQF 643

BLAST of Lsi04G003440 vs. NCBI nr
Match: XP_023542840.1 (phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo] >XP_023542841.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 630/645 (97.67%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSPVQTQMAVAALKSPLSR+YR+RNGM+EK PVGRRQVFVQTDTGCVLGMELDRN
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYRARNGMEEKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQ+ALNIPTEESSLTFGDVILKNDL+AIR+DSPLLLTRNL+HRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQIALNIPTEESSLTFGDVILKNDLTAIRSDSPLLLTRNLMHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHS+RFSGT+RLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTRRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKP  HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKP--HKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFS+DELKYIENL+PIKDCEMLRMELPMIR+ACLRVLVLCTIFLKEAAA GLCL
Sbjct: 361 WPQASIPFSEDELKYIENLNPIKDCEMLRMELPMIRDACLRVLVLCTIFLKEAAACGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNEEFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERE CSP  DW N+EFQFDLDCE+ EL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAERELCSPVDDWGNDEFQFDLDCEEREL 480

Query: 481 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 540
           DYNS +AADQCLGWSHYQFGGLG +HGRNPLSKLEESIEEEGEDS GEEQGETATF PSE
Sbjct: 481 DYNSSVAADQCLGWSHYQFGGLGGMHGRNPLSKLEESIEEEGEDSAGEEQGETATFPPSE 540

Query: 541 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600
           PIS V+KLSMSLKNTILGDKNQK QKYQV+KPEYGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 PISAVTKLSMSLKNTILGDKNQKHQKYQVSKPEYGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           GDMN+EEW+LFLDKFPE+LRPAFAKR++AA+GQRQRQRLGTSCQF
Sbjct: 601 GDMNDEEWTLFLDKFPEVLRPAFAKRQSAALGQRQRQRLGTSCQF 643

BLAST of Lsi04G003440 vs. TAIR 10
Match: AT2G03890.1 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 877.1 bits (2265), Expect = 8.7e-255
Identity = 459/666 (68.92%), Postives = 529/666 (79.43%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+   +       K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRNDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDR+DN HTVKRRLQ+ALN PTEESSLT+GD++L NDLSA+RNDSPLLL RN +HRSS
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRSS 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGA 180
           STPCLSPTG+DL+ +D+SGPIEILGHSD FS  K +VK+IVKA+K+G++P+PVHSGLGGA
Sbjct: 121 STPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGGA 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
           YYFRN +GESVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLD
Sbjct: 181 YYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP SD   E EFQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGET 540
           DC+D E  Y+S +        +   F      +GR+ L KLEESI+EE ED   EE+ +T
Sbjct: 481 DCDDLESVYSSKIQL------TDDYFTKNPFSNGRSSLGKLEESIKEEEEDE-EEEEDKT 540

Query: 541 ATFLP--------------SEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITN 600
              +P               +    +SKLS S+KNT L D  +K  K  +T+   G   N
Sbjct: 541 ENTVPMIIMKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKSEN 600

Query: 601 TSAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRL 646
           TS+GH+SANEQ P S SFVK+ DM E+EW LFL++F ELL PAFAKRK A +   +RQRL
Sbjct: 601 TSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRL 650

BLAST of Lsi04G003440 vs. TAIR 10
Match: AT1G26270.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 864.0 bits (2231), Expect = 7.7e-251
Identity = 447/649 (68.88%), Postives = 523/649 (80.59%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEKQPVGRRQVFVQTDTGCVLGMELDRN 60
           MSRKLDSP++TQMAVA +KSPL+  +R  N +  K PVGRR+VFVQT+TGCVLG+ELDR+
Sbjct: 1   MSRKLDSPIKTQMAVALVKSPLNGEFREFNKVGMKTPVGRRRVFVQTETGCVLGLELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKR+LQ+ALN P EESSLTFGD++LKNDL+A+R+DSPLLLTRN  HRSSSTPCLS
Sbjct: 61  DNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPCLS 120

Query: 121 PTGKDL-RLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRN 180
           P   DL + RD+S PIEILG+S  FS  +++ K+I KA+K GIDP+ V+SGLGGAYYF+N
Sbjct: 121 PMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYFKN 180

Query: 181 GKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFA 240
            +GESVAIVKPTDEEP+APNNPKGFVGKALGQPG KRSVRVGETG+REVAAYLLD EHFA
Sbjct: 181 SRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEHFA 240

Query: 241 NVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPV 300
           NVPPTALVKITHSIFNVND V  +KP   +K  VSKIAS QQFIPHD+DAS+HGTS+FPV
Sbjct: 241 NVPPTALVKITHSIFNVNDGVKASKPM--EKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 301 AAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWI 360
           +AVHRIGILDIRI NTDRH+GNLLV+KLDG G FGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 361 HWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLC 420
           HWPQASIPFS+DELKYI NLDP+ DCEMLR ELPM+REA LRVLVLCTIFLKEAAA+GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 421 LAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDLDCEDG 480
           LAEIGEMM+RE R G+EEPSE+E+VCLEA  +I E++  SP SD  N+ EFQFD+DCE+ 
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEE- 480

Query: 481 ELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIE--EEGEDSGGEEQGETATF 540
             D    +A             GL   + R  LSK+EE+ E  EE E+   EE+      
Sbjct: 481 VTDCTKKLALPL----------GLTFGNARGQLSKVEETTEDGEEEEEEDREEEENDRAD 540

Query: 541 LPSEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVS 600
           L   P     KLSMSLK+T+LG+K+QK QK+   + E  Y    S+ HRSA+EQ P S S
Sbjct: 541 LEKMP---TIKLSMSLKSTLLGEKSQKYQKHPGARVESAY---ASSAHRSADEQIPSSTS 600

Query: 601 FVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
           FVKL DM+EEEW++FL+K+ ELL PA  KRK+  +GQ+QRQRLGTSCQF
Sbjct: 601 FVKLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of Lsi04G003440 vs. TAIR 10
Match: AT1G13640.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 815.8 bits (2106), Expect = 2.4e-236
Identity = 422/645 (65.43%), Postives = 497/645 (77.05%), Query Frame = 0

Query: 13  MAVAALKSPLSRNYRSRNGMD---------EKQPVGRRQVFVQTDTGCVLGMELDRNDNA 72
           MA+A  K+PL   +     M+         ++Q  GRR+VFVQTDTGCVLG+ELDRNDN 
Sbjct: 1   MAMAVFKAPLKGEFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNV 60

Query: 73  HTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTG 132
           HTVK+RLQ+A N PTEESSLTFGD++LKNDLSA+RNDSPLLL RNL+HRSSSTPCLSPTG
Sbjct: 61  HTVKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTG 120

Query: 133 KDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNGKGE 192
            DL+ +D+SGPIEIL HS  F   K+   +IVKA+K+G++PIPV+ GLGGAYYFR+ KG+
Sbjct: 121 NDLQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQ 180

Query: 193 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPP 252
           SVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLDY+HFANVPP
Sbjct: 181 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPP 240

Query: 253 TALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVAAVH 312
           TALVKITHS+FNVND ++GNK +  KK   SKIASFQ+F+PHDFDASDHGTSSFPVA+VH
Sbjct: 241 TALVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVH 300

Query: 313 RIGILDIRIFNTDRHAGNLLVRKLD--GIGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 372
           RIGILDIRI NTDRH GNLLV+KLD  G+GRFGQVELIPIDHGLCLPETLEDPYFEWIHW
Sbjct: 301 RIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 360

Query: 373 PQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLA 432
           PQASIPFS++EL YI++LDP+KDCEMLR ELPMIREACLRVLVLCT+FLKEAA  GLCLA
Sbjct: 361 PQASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLA 420

Query: 433 EIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDLDCEDGEL 492
           EIGEMM+REFR+GEEEPSELE++C+EA+++  E++  SP SD   E EFQFD+D  + + 
Sbjct: 421 EIGEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDS 480

Query: 493 DYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGETATFLPSE 552
            Y S    D+    + +        +GR+ L +L+ESI EE ED   EE+ +    L   
Sbjct: 481 VYGSETETDEFFAKNPFS-------NGRSSLGELKESIAEEEEDD--EEEAKLTLSL--- 540

Query: 553 PISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVKL 612
                SKLS S+KN +    N     Y +  P+          H+SAN Q P SV+FVKL
Sbjct: 541 -----SKLSTSMKNNL---SNTMGSGY-LKPPKDNQTDKALVSHKSANVQLPLSVNFVKL 600

Query: 613 GDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLGTSCQF 646
            DM E EW +FL++F ELL  AFA+RK   M  R  QRLGTSC+F
Sbjct: 601 ADMKEVEWVVFLERFQELLYSAFAERK--TMTLRNTQRLGTSCKF 622

BLAST of Lsi04G003440 vs. TAIR 10
Match: AT2G03890.2 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 620.2 bits (1598), Expect = 1.9e-177
Identity = 362/666 (54.35%), Postives = 419/666 (62.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRNYRSRNGMDEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+   +       K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRNDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDR+DN HTVKRRLQ+ALN PTEESSLT+GD++L NDLS+                  
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSS------------------ 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGA 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
                                                     SVRVGETGFREVAAYLLD
Sbjct: 181 ------------------------------------------SVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTSDWSNE-EFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP SD   E EFQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEGEDSGGEEQGET 540
           DC+D E  Y+S +        +   F      +GR+ L KLEESI+EE ED   EE+ +T
Sbjct: 481 DCDDLESVYSSKIQL------TDDYFTKNPFSNGRSSLGKLEESIKEEEEDE-EEEEDKT 530

Query: 541 ATFLP--------------SEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITN 600
              +P               +    +SKLS S+KNT L D  +K  K  +T+   G   N
Sbjct: 541 ENTVPMIIMKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKSEN 530

Query: 601 TSAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRL 646
           TS+GH+SANEQ P S SFVK+ DM E+EW LFL++F ELL PAFAKRK A +   +RQRL
Sbjct: 601 TSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRL 530

BLAST of Lsi04G003440 vs. TAIR 10
Match: AT2G40850.1 (phosphoinositide 4-kinase gamma 1 )

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-103
Identity = 229/544 (42.10%), Postives = 306/544 (56.25%), Query Frame = 0

Query: 110 LHRSSSTPCLS---PTGKDLRLRDQSGP-IEILGHSDRFSGTKRLVKEIVKAIKLGIDPI 169
           +HRS S+PC S       +  L+  + P IEILG   R    + LV E+  A+  G  P+
Sbjct: 67  IHRSVSSPCFSIAASANMEEDLKATTAPRIEILG-GQRVPTVRALVAEVTMAMVSGAQPL 126

Query: 170 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 229
            + SG+GGAY  + GKG ++A+ KP DEEP A NNPK      LGQPG K S+ VGETG 
Sbjct: 127 LLPSGMGGAYLLQTGKGHNIAVAKPVDEEPLAFNNPKKSGNLMLGQPGMKHSIPVGETGI 186

Query: 230 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQGHKKKSVSKIASFQQFIPH 289
           RE+AAYLLDY+ F+ VPPTALV I+H  F+V+D  + +           K+AS Q+F+ H
Sbjct: 187 RELAAYLLDYQGFSGVPPTALVSISHVPFHVSDAFSFS-------SMPYKVASLQRFVGH 246

Query: 290 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLD---GIGRFGQVELIPID 349
           DFDA + G  SF   +VHRIGILD+R+ N DRHAGN+LV++ D      R G  EL+PID
Sbjct: 247 DFDAGELGPGSFTATSVHRIGILDVRLLNLDRHAGNMLVKRCDKKEAYNRLGTAELVPID 306

Query: 350 HGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRV 409
           HGLCLPE L+DPYFEW++WPQA +PFSD EL YI NLDP KD E+LR EL  + E+ +RV
Sbjct: 307 HGLCLPECLDDPYFEWLNWPQALVPFSDTELDYISNLDPFKDAELLRTELHSLPESAIRV 366

Query: 410 LVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFCSPTS 469
           LV+CT+FLK+AAA+GLCLAEIGE M+R+F  GEE  S LE +C +A+  +  +   S  S
Sbjct: 367 LVVCTVFLKQAAAAGLCLAEIGEKMTRDFSKGEESFSLLETLCTKAKASVFGK--TSEDS 426

Query: 470 DWSNEEFQFDLDCEDGELDYNSGMAADQCLGWSHYQFGGLGSLHGRNPLSKLEESIEEEG 529
           D+S+E  + + + + G   ++ G                          +  E  I E  
Sbjct: 427 DYSHEGNEVNTELQCGMFKFDGGD-------------------------TPCEAEISEVF 486

Query: 530 EDSGGE--EQGETATFLPSEPISVVSKLSMSLKNTILGDKNQKTQKYQVTKPEYGYITNT 589
             S      +G  A  +P++   V + +S S    I   +    +K +  K E   + + 
Sbjct: 487 HVSKPPLVPRGPRANTIPND---VTASMSSSQNQRITHQEKSAKEKKRGGKQERCTVRSK 546

Query: 590 SAGHRSANEQFPPSVSFVKLGDMNEEEWSLFLDKFPELLRPAFAKRKAAAMGQRQRQRLG 645
           S    S N      VSFV   DM   EW  FL  F  LL+ A +K            RLG
Sbjct: 547 SP-PISPNHDESKGVSFV---DMTTVEWDTFLQSFQTLLQDALSKGST--------PRLG 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SI521.2e-25368.92Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 ... [more]
Q9C6711.1e-24968.88Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 ... [more]
Q8W4R83.4e-23565.43Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 ... [more]
O221991.5e-10242.10Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 ... [more]
Q0WMZ61.1e-10051.03Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 ... [more]
Match NameE-valueIdentityDescription
A0A1S3BFU20.0e+0098.141-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103489203 PE=3 SV=... [more]
A0A5D3CB140.0e+0098.141-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KWA50.0e+0096.901-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G606910 PE=3 ... [more]
A0A6J1HTY70.0e+0093.951-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111467446 PE=3... [more]
A0A6J1FZC80.0e+0093.951-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111449302 PE... [more]
Match NameE-valueIdentityDescription
XP_008446479.10.0e+0098.14PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >XP_008446480.1 ... [more]
XP_004135137.10.0e+0096.90phosphatidylinositol 4-kinase gamma 5 [Cucumis sativus] >XP_004135138.1 phosphat... [more]
KAG6574027.10.0e+0093.95Phosphatidylinositol 4-kinase gamma 5, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022968111.10.0e+0093.95phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022968112.1 ph... [more]
XP_023542840.10.0e+0093.95phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
AT2G03890.18.7e-25568.92phosphoinositide 4-kinase gamma 7 [more]
AT1G26270.17.7e-25168.88Phosphatidylinositol 3- and 4-kinase family protein [more]
AT1G13640.12.4e-23665.43Phosphatidylinositol 3- and 4-kinase family protein [more]
AT2G03890.21.9e-17754.35phosphoinositide 4-kinase gamma 7 [more]
AT2G40850.11.1e-10342.10phosphoinositide 4-kinase gamma 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPFAMPF00454PI3_PI4_kinasecoord: 171..423
e-value: 1.5E-49
score: 169.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 515..536
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..40
NoneNo IPR availablePANTHERPTHR45800:SF11PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 7coord: 6..645
NoneNo IPR availableCDDcd17039Ubl_ubiquitin_likecoord: 43..105
e-value: 6.6535E-4
score: 36.4202
IPR044571Phosphatidylinositol 4-kinase gamma 1-8PANTHERPTHR45800PHOSPHATIDYLINOSITOL 4-KINASE GAMMAcoord: 6..645
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 41..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G003440.1Lsi04G003440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0016310 phosphorylation
molecular_function GO:0004430 1-phosphatidylinositol 4-kinase activity