Lsi04G003390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G003390
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlycosyltransferase
Locationchr04: 3410018 .. 3411418 (+)
RNA-Seq ExpressionLsi04G003390
SyntenyLsi04G003390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCGTTGCCTTCGTCGCCATTACCCCACCTGCTGGTTTTTCCATACCCAGCTCAGGGCCATATGCTCCCACTCCTCGATCTCACCCATTTCTTAGCTTCTTATGGCTTCACCATCACTGTTTTGGTTACCCCCAAAAACCTCCCCATCCTTCAACCCCTTCTTTCAGCCCACCCCTTAGTTCAAACCCTTGTTCTCCCTTTCCCTTCTGTCCCTGATTTGCCTCCCGGTGTTGAAAATGTCAAAGACATCGGCGGCCAAGGCAACGTCCCCATCATGGTGGCCCTCCGCCAACTCCAAGACCCCATTGTTGAGTGGTTCAAATCCCACCCTTCACCTCCCTCCGCCATTATTGCTGATTTCTTCCTCGGATGGACCGAATCCCTCGCCCGTAAGCTTCAAATCCCCAGAATCACTTTCTACTCCTCTGGTGCTTGGGTTGTCAATATCCTCGATTACTGTTGGCGTTACGTACAATCTGAAGATTTCCGTAAATCTCCAGTCAAGCATATGGCTGAATTGCCCAATTCCCCCACCTTCACTAATCAGGATTTGCCGGAATTTGTCACTATATACGAAGATTCTGACCCCCGATGGCATGCTATAAGATCCGACTGGCTTGAAACTAGTACCTGTTGGGCGGGTGTTTTCAATACCTTCGAGGCGTTGGAGCAGGACTATTTGGACTACTTCAGGAAATTAAACGGCGGGAGTGTATTTGGAGTCGGACCGCTAAGCCTCATCCATAGCCACAGCCCACTTGCAGATTCGAGTTCAGATGAGATACTGAAATGGCTCGACGGATGCCCTGATGATTCGGTGCTATACGTTAGCTTTGGGAGTCAAAAGCAGCTGAATCAACAGCAAATGGAAGCACTAGCATCCGGGCTTGAGAAAAGCGGGATTCGATTCCTGTGGGTGGTTAAGACAATTCATCAAACAGTTGGTGAGTCGGACGGAATCCCAATTGGGTTTGAGGATCGGGTGTTGGATCGTGGAATCGTGGTAAGGAGATGGGCTCCGCAGGTGGCGATACTGAATCACCGAGCCGTGGGAGGGTTTCTGAGCCACTGCGGGTGGAACTCTGTACTGGAGAGCTTAGTGAGCGGAGTTCCAATATTTGCTTGGGCCATGGAAGGGGATCAAATGATGAACTCAAAGATTTTGGTGGATCACGTTGGAGTGGCGGTGCAAGTGTGCCATGGAGAGAACTCGGTGCCGGACTCGGACCAACTGGGGAAGGTCATCGCCGAGTCTTTCAACTCCGACGAACTGAAAGCTAAAGCCAAAGCTCTGAGCAAAGCTGTGGTGGACGCAGCTGGCCCCAATGGAAGTTCAGTGAGAGCCCTCCAAGAATTTGCCAATAAGCTGGCTACCCTTCCTCCACCATCCAATTGA

mRNA sequence

ATGGCATCGTTGCCTTCGTCGCCATTACCCCACCTGCTGGTTTTTCCATACCCAGCTCAGGGCCATATGCTCCCACTCCTCGATCTCACCCATTTCTTAGCTTCTTATGGCTTCACCATCACTGTTTTGGTTACCCCCAAAAACCTCCCCATCCTTCAACCCCTTCTTTCAGCCCACCCCTTAGTTCAAACCCTTGTTCTCCCTTTCCCTTCTGTCCCTGATTTGCCTCCCGGTGTTGAAAATGTCAAAGACATCGGCGGCCAAGGCAACGTCCCCATCATGGTGGCCCTCCGCCAACTCCAAGACCCCATTGTTGAGTGGTTCAAATCCCACCCTTCACCTCCCTCCGCCATTATTGCTGATTTCTTCCTCGGATGGACCGAATCCCTCGCCCGTAAGCTTCAAATCCCCAGAATCACTTTCTACTCCTCTGGTGCTTGGGTTGTCAATATCCTCGATTACTGTTGGCGTTACGTACAATCTGAAGATTTCCGTAAATCTCCAGTCAAGCATATGGCTGAATTGCCCAATTCCCCCACCTTCACTAATCAGGATTTGCCGGAATTTGTCACTATATACGAAGATTCTGACCCCCGATGGCATGCTATAAGATCCGACTGGCTTGAAACTAGTACCTGTTGGGCGGGTGTTTTCAATACCTTCGAGGCGTTGGAGCAGGACTATTTGGACTACTTCAGGAAATTAAACGGCGGGAGTGTATTTGGAGTCGGACCGCTAAGCCTCATCCATAGCCACAGCCCACTTGCAGATTCGAGTTCAGATGAGATACTGAAATGGCTCGACGGATGCCCTGATGATTCGGTGCTATACGTTAGCTTTGGGAGTCAAAAGCAGCTGAATCAACAGCAAATGGAAGCACTAGCATCCGGGCTTGAGAAAAGCGGGATTCGATTCCTGTGGGTGGTTAAGACAATTCATCAAACAGTTGGTGAGTCGGACGGAATCCCAATTGGGTTTGAGGATCGGGTGTTGGATCGTGGAATCGTGGTAAGGAGATGGGCTCCGCAGGTGGCGATACTGAATCACCGAGCCGTGGGAGGGTTTCTGAGCCACTGCGGGTGGAACTCTGTACTGGAGAGCTTAGTGAGCGGAGTTCCAATATTTGCTTGGGCCATGGAAGGGGATCAAATGATGAACTCAAAGATTTTGGTGGATCACGTTGGAGTGGCGGTGCAAGTGTGCCATGGAGAGAACTCGGTGCCGGACTCGGACCAACTGGGGAAGGTCATCGCCGAGTCTTTCAACTCCGACGAACTGAAAGCTAAAGCCAAAGCTCTGAGCAAAGCTGTGGTGGACGCAGCTGGCCCCAATGGAAGTTCAGTGAGAGCCCTCCAAGAATTTGCCAATAAGCTGGCTACCCTTCCTCCACCATCCAATTGA

Coding sequence (CDS)

ATGGCATCGTTGCCTTCGTCGCCATTACCCCACCTGCTGGTTTTTCCATACCCAGCTCAGGGCCATATGCTCCCACTCCTCGATCTCACCCATTTCTTAGCTTCTTATGGCTTCACCATCACTGTTTTGGTTACCCCCAAAAACCTCCCCATCCTTCAACCCCTTCTTTCAGCCCACCCCTTAGTTCAAACCCTTGTTCTCCCTTTCCCTTCTGTCCCTGATTTGCCTCCCGGTGTTGAAAATGTCAAAGACATCGGCGGCCAAGGCAACGTCCCCATCATGGTGGCCCTCCGCCAACTCCAAGACCCCATTGTTGAGTGGTTCAAATCCCACCCTTCACCTCCCTCCGCCATTATTGCTGATTTCTTCCTCGGATGGACCGAATCCCTCGCCCGTAAGCTTCAAATCCCCAGAATCACTTTCTACTCCTCTGGTGCTTGGGTTGTCAATATCCTCGATTACTGTTGGCGTTACGTACAATCTGAAGATTTCCGTAAATCTCCAGTCAAGCATATGGCTGAATTGCCCAATTCCCCCACCTTCACTAATCAGGATTTGCCGGAATTTGTCACTATATACGAAGATTCTGACCCCCGATGGCATGCTATAAGATCCGACTGGCTTGAAACTAGTACCTGTTGGGCGGGTGTTTTCAATACCTTCGAGGCGTTGGAGCAGGACTATTTGGACTACTTCAGGAAATTAAACGGCGGGAGTGTATTTGGAGTCGGACCGCTAAGCCTCATCCATAGCCACAGCCCACTTGCAGATTCGAGTTCAGATGAGATACTGAAATGGCTCGACGGATGCCCTGATGATTCGGTGCTATACGTTAGCTTTGGGAGTCAAAAGCAGCTGAATCAACAGCAAATGGAAGCACTAGCATCCGGGCTTGAGAAAAGCGGGATTCGATTCCTGTGGGTGGTTAAGACAATTCATCAAACAGTTGGTGAGTCGGACGGAATCCCAATTGGGTTTGAGGATCGGGTGTTGGATCGTGGAATCGTGGTAAGGAGATGGGCTCCGCAGGTGGCGATACTGAATCACCGAGCCGTGGGAGGGTTTCTGAGCCACTGCGGGTGGAACTCTGTACTGGAGAGCTTAGTGAGCGGAGTTCCAATATTTGCTTGGGCCATGGAAGGGGATCAAATGATGAACTCAAAGATTTTGGTGGATCACGTTGGAGTGGCGGTGCAAGTGTGCCATGGAGAGAACTCGGTGCCGGACTCGGACCAACTGGGGAAGGTCATCGCCGAGTCTTTCAACTCCGACGAACTGAAAGCTAAAGCCAAAGCTCTGAGCAAAGCTGTGGTGGACGCAGCTGGCCCCAATGGAAGTTCAGTGAGAGCCCTCCAAGAATTTGCCAATAAGCTGGCTACCCTTCCTCCACCATCCAATTGA

Protein sequence

MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHPLVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGGSVFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEKSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAESFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPSN
Homology
BLAST of Lsi04G003390 vs. ExPASy Swiss-Prot
Match: Q9LZD8 (UDP-glycosyltransferase 89A2 OS=Arabidopsis thaliana OX=3702 GN=UGT89A2 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.1e-118
Identity = 231/464 (49.78%), Postives = 304/464 (65.52%), Query Frame = 0

Query: 6   SSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP-LVQT 65
           +S  PH++VFP+PAQGH+LPLLDLTH L   GF ++V+VTP NL  L PLLSAHP  V +
Sbjct: 14  NSKPPHIVVFPFPAQGHLLPLLDLTHQLCLRGFNVSVIVTPGNLTYLSPLLSAHPSSVTS 73

Query: 66  LVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFL 125
           +V PFP  P L PGVENVKD+G  GN+PIM +LRQL++PI+ WF+SHP+PP A+I+DFFL
Sbjct: 74  VVFPFPPHPSLSPGVENVKDVGNSGNLPIMASLRQLREPIINWFQSHPNPPIALISDFFL 133

Query: 126 GWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKS--PVKHMAELPNSPTFT 185
           GWT  L  ++ IPR  F+S   ++V++L +C+  +   D  KS  P+ H+ +LP +P F 
Sbjct: 134 GWTHDLCNQIGIPRFAFFSISFFLVSVLQFCFENI---DLIKSTDPI-HLLDLPRAPIFK 193

Query: 186 NQDLPEFV-TIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSV 245
            + LP  V    +   P   +I+ D+      +  VFN+ E LE DYL Y ++  G   V
Sbjct: 194 EEHLPSIVRRSLQTPSPDLESIK-DFSMNLLSYGSVFNSSEILEDDYLQYVKQRMGHDRV 253

Query: 246 FGVGPLSLIHSHSPLADSSSD-EILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 305
           + +GPL  I S       S D  +L WLDG P+ SVLYV FGSQK L + Q +ALA GLE
Sbjct: 254 YVIGPLCSIGSGLKSNSGSVDPSLLSWLDGSPNGSVLYVCFGSQKALTKDQCDALALGLE 313

Query: 306 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 365
           KS  RF+WVVK         D IP GFEDRV  RG+VVR W  Q+A+L H AVGGFLSHC
Sbjct: 314 KSMTRFVWVVK--------KDPIPDGFEDRVSGRGLVVRGWVSQLAVLRHVAVGGFLSHC 373

Query: 366 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 425
           GWNSVLE + SG  I  W ME DQ +N+++LV+H+GVAV+VC G  +VPDSD+LG+VIAE
Sbjct: 374 GWNSVLEGITSGAVILGWPMEADQFVNARLLVEHLGVAVRVCEGGETVPDSDELGRVIAE 433

Query: 426 SFNSD--ELKAKAKALSK----AVVDAAGPNGSSVRALQEFANK 458
           +      E+ A+A+ + +    AV +A   NGSSV  +Q    +
Sbjct: 434 TMGEGGREVAARAEEIRRKTEAAVTEA---NGSSVENVQRLVKE 461

BLAST of Lsi04G003390 vs. ExPASy Swiss-Prot
Match: Q6VAA5 (UDP-glycosyltransferase 89B2 OS=Stevia rebaudiana OX=55670 GN=UGT89B2 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 9.0e-105
Identity = 208/460 (45.22%), Postives = 282/460 (61.30%), Query Frame = 0

Query: 11  HLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP-LVQTLVLPF 70
           H+LVFPYPAQGHML LLDLTH LA    TIT+LVTPKNLP + PLL+AHP  V  L+LP 
Sbjct: 11  HILVFPYPAQGHMLTLLDLTHQLAIRNLTITILVTPKNLPTISPLLAAHPTTVSALLLPL 70

Query: 71  PSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFLGWTES 130
           P  P +P G+ENVKD+       +MVAL  L +P+ +WF++ P+PP AII+DFFLGWT  
Sbjct: 71  PPHPAIPSGIENVKDLPNDAFKAMMVALGDLYNPLRDWFRNQPNPPVAIISDFFLGWTHH 130

Query: 131 LARKLQIPRITFYSSGAWVVNILDYCWRYVQSE---DFRKSPVKHMAELPNSPTFTNQDL 190
           LA +L I R TF  SGA  ++++   WRY       +  K  +K   ++PNSP +    L
Sbjct: 131 LAVELGIRRYTFSPSGALALSVIFSLWRYQPKRIDVENEKEAIK-FPKIPNSPEYPWWQL 190

Query: 191 PEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFR-KLNGGSVFGVGP 250
                 Y + DP    I+  +L     W  V N+F  LEQ Y+D+ + +L    VF VGP
Sbjct: 191 SPIYRSYVEGDPDSEFIKDGFLADIASWGIVINSFTELEQVYVDHLKHELGHDQVFAVGP 250

Query: 251 -LSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEKSGIR 310
            L      S    SSS+++L WLD C D +V+YV FGSQ  L   QME +A GLEKS ++
Sbjct: 251 LLPPGDKTSGRGGSSSNDVLSWLDTCADRTVVYVCFGSQMVLTNGQMEVVALGLEKSRVK 310

Query: 311 FLWVVK--TIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCGWN 370
           F+W VK  T+         +P GFEDRV  RG+V+R W PQVAIL+H +VG FL+HCGWN
Sbjct: 311 FVWSVKEPTVGHEAANYGRVPPGFEDRVSGRGLVIRGWVPQVAILSHDSVGVFLTHCGWN 370

Query: 371 SVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAESFN 430
           SV+E++ + V +  W M  DQ  N+ +L   + V ++VC G N VP+SD+L ++ ++S  
Sbjct: 371 SVMEAVAAEVLMLTWPMSADQFSNATLL-HELKVGIKVCEGSNIVPNSDELAELFSKSL- 430

Query: 431 SDEL---KAKAKALSKAVVDAAGPNGSSVRALQEFANKLA 460
           SDE    + + K  +K+  +A GP GSSV  L+   + L+
Sbjct: 431 SDETRLERKRVKEFAKSAKEAVGPKGSSVGELERLVDNLS 467

BLAST of Lsi04G003390 vs. ExPASy Swiss-Prot
Match: Q9C9B0 (Flavonol 3-O-glucosyltransferase UGT89B1 OS=Arabidopsis thaliana OX=3702 GN=UGT89B1 PE=1 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 2.2e-103
Identity = 202/461 (43.82%), Postives = 279/461 (60.52%), Query Frame = 0

Query: 11  HLLVFPYPAQGHMLPLLDLTHFLASYG---FTITVLVTPKNLPILQPLLSAHPLVQTLVL 70
           H+L+FP+PAQGHM+PLLD TH LA  G     ITVLVTPKNLP L PLLSA   ++ L+L
Sbjct: 14  HVLIFPFPAQGHMIPLLDFTHRLALRGGAALKITVLVTPKNLPFLSPLLSAVVNIEPLIL 73

Query: 71  PFPSVPDLPPGVENVKDIGGQGNVPIMV-ALRQLQDPIVEWFKSHPSPPSAIIADFFLGW 130
           PFPS P +P GVENV+D+   G  P+M+ AL  L  P++ W  SHPSPP AI++DFFLGW
Sbjct: 74  PFPSHPSIPSGVENVQDLPPSG-FPLMIHALGNLHAPLISWITSHPSPPVAIVSDFFLGW 133

Query: 131 TESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSE--DFRKSPVKHMAELPNSPTFTNQ 190
           T++L     IPR  F  S A    IL+  W  + ++  +   + + H  ++PN P +   
Sbjct: 134 TKNLG----IPRFDFSPSAAITCCILNTLWIEMPTKINEDDDNEILHFPKIPNCPKYRFD 193

Query: 191 DLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYF-RKLNGGSVFGV 250
            +      Y   DP W  IR  + +    W  V N+F A+E  YL++  R++    V+ V
Sbjct: 194 QISSLYRSYVHGDPAWEFIRDSFRDNVASWGLVVNSFTAMEGVYLEHLKREMGHDRVWAV 253

Query: 251 GPLSLI--HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEKS 310
           GP+  +   +       S D ++ WLD   D+ V+YV FGSQ  L ++Q  ALASGLEKS
Sbjct: 254 GPIIPLSGDNRGGPTSVSVDHVMSWLDAREDNHVVYVCFGSQVVLTKEQTLALASGLEKS 313

Query: 311 GIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCGW 370
           G+ F+W VK   +       I  GF+DRV  RG+V+R WAPQVA+L HRAVG FL+HCGW
Sbjct: 314 GVHFIWAVKEPVEKDSTRGNILDGFDDRVAGRGLVIRGWAPQVAVLRHRAVGAFLTHCGW 373

Query: 371 NSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAESF 430
           NSV+E++V+GV +  W M  DQ  ++ ++VD + V V+ C G ++VPD D+L +V A+S 
Sbjct: 374 NSVVEAVVAGVLMLTWPMRADQYTDASLVVDELKVGVRACEGPDTVPDPDELARVFADSV 433

Query: 431 NSDEL-KAKAKALSKAVVDAAGPNGSSVRALQEFANKLATL 462
             ++  + KA  L KA +DA    GSSV  L  F   + +L
Sbjct: 434 TGNQTERIKAVELRKAALDAIQERGSSVNDLDGFIQHVVSL 469

BLAST of Lsi04G003390 vs. ExPASy Swiss-Prot
Match: Q9LNE6 (Flavonol 7-O-rhamnosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT89C1 PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 8.5e-71
Identity = 173/469 (36.89%), Postives = 247/469 (52.67%), Query Frame = 0

Query: 10  PHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAH--PLVQTLVL 69
           PH+LV P+P  GHM+P LDLTH +   G T+TVLVTPKN   L  L S H     +TL+L
Sbjct: 9   PHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLIL 68

Query: 70  PFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSP--PSAIIADFFLG 129
           PFPS P +P GVE+++ +  +  V +  AL +L DP+V++    P    P AI+   FL 
Sbjct: 69  PFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLS 128

Query: 130 -WTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTNQ 189
            W   +A    I  I+F    A  ++++   W     ED                +F N 
Sbjct: 129 PWINKVADAFSIKSISFLPINAHSISVM---W---AQED---------------RSFFND 188

Query: 190 DLPEFVTIYEDSDPRWHAIRSDWLETST--CWAGVFNTFEALEQDYLDYF--RKLNGGSV 249
                                  LET+T   +  V N+F  LE ++++    R LN   +
Sbjct: 189 -----------------------LETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRI 248

Query: 250 FGVGPLSLIHSHSPLADSSS---DEILKWLDGCPDD-SVLYVSFGSQKQLNQQQMEALAS 309
           + VGPL    +       SS    ++  WLD CP+D SV+YV FGSQ +L  +Q  ALA+
Sbjct: 249 WTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAA 308

Query: 310 GLEKSGIRFLWVVKTIHQTVGESDG------IPIGFEDRVLDRGIVVRRWAPQVAILNHR 369
            LEKS +RF+W V+   + V  SD       IP GFE+RV ++G+V+R WAPQ  IL HR
Sbjct: 309 ALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHR 368

Query: 370 AVGGFLSHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDS 429
           AVG +L+H GW SVLE +V GV + AW M+ D   N+ ++VD +  AV+V    +SVPDS
Sbjct: 369 AVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDS 428

Query: 430 DQLGKVIAESFNSD-ELKAKAKALSKAVVDAAGPNGSSVRALQEFANKL 459
           D+L +++AES   D   +     L +  ++A    GSS + L E   ++
Sbjct: 429 DKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEM 433

BLAST of Lsi04G003390 vs. ExPASy Swiss-Prot
Match: K4GKX2 (UDP-glycosyltransferase 73C10 OS=Barbarea vulgaris OX=50459 GN=UGT73C10 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.2e-62
Identity = 156/497 (31.39%), Postives = 250/497 (50.30%), Query Frame = 0

Query: 6   SSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLP----ILQPLLSAHPL 65
           S PL H ++FP+ AQGHM+P++D+   LA  G  IT++ TP N      +L   + +   
Sbjct: 9   SYPL-HFVLFPFMAQGHMIPMVDIARLLAQRGVKITIVTTPHNAARFENVLSRAIESGLP 68

Query: 66  VQTLVLPFPS-VPDLPPGVENVKD-IGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAII 125
           +  + +  PS    LP G E     +  +  VP   A+  L++P+ + F+     PS II
Sbjct: 69  ISIVQVKLPSQEAGLPEGNETFDSLVSTKLLVPFFKAVNMLEEPVQKLFEEMSPQPSCII 128

Query: 126 ADFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKH--MAELPN 185
           +DF L +T  +A+K  IP+I F+    + +  +    +  +  +  KS  +H  +   P+
Sbjct: 129 SDFCLPYTSKIAKKFNIPKILFHGMCCFCLLCMHVLRKNREILENLKSDKEHFVVPYFPD 188

Query: 186 SPTFTNQDLPEFVTIYEDSDPRWHAIRSDWLET-STCWAGVFNTFEALEQDYLDYFRKLN 245
              FT   +P  +  Y   +  WH I+ D +E   T +  + NT++ LE  Y + +++  
Sbjct: 189 RVEFTRPQVP--LATYVPGE--WHEIKEDMVEADKTSYGVIVNTYQELEPAYANGYKEAR 248

Query: 246 GGSVFGVGPLSLIH-------SHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQ 305
            G  + +GP+SL +            AD   DE LKWLD   + SVLYV  GS   L   
Sbjct: 249 SGKAWTIGPVSLCNKVGADKAERGNKADIDQDECLKWLDSKEEGSVLYVCLGSICSLPLS 308

Query: 306 QMEALASGLEKSGIRFLWVV------KTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQ 365
           Q++ L  GLE+S   F+WVV      K + +   ES     GFE+RV DRG++++ W+PQ
Sbjct: 309 QLKELGLGLEESQRPFIWVVRGWEKNKELLEWFSES-----GFEERVKDRGLLIKGWSPQ 368

Query: 366 VAILNHRAVGGFLSHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHG 425
           + IL H +VGGFL+HCGWNS LE + SGVP+  W + GDQ  N K++V  + V V     
Sbjct: 369 MLILAHHSVGGFLTHCGWNSTLEGITSGVPLLTWPLFGDQFCNQKLVVQVLKVGVSAGVE 428

Query: 426 E-NSVPDSDQLG-------------KVIAESFNSDELKAKAKALSKAVVDAAGPNGSSVR 467
           E  +  + +++G             +++ ES ++ E++ + K L +    A    GSS  
Sbjct: 429 EVTNWGEEEKIGVLVDKEGVKKAVEELMGESDDAKEIRKRVKELGQLAHKAVEEGGSSHS 488

BLAST of Lsi04G003390 vs. ExPASy TrEMBL
Match: A0A6J1GYG2 (Glycosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111458431 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 7.4e-227
Identity = 377/466 (80.90%), Postives = 424/466 (90.99%), Query Frame = 0

Query: 1   MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP 60
           MAS PSSPLPHLL+FPYPAQGHMLPLLDLTHFLASYGF ITVLVTPKNLPILQPLLSAHP
Sbjct: 1   MAS-PSSPLPHLLLFPYPAQGHMLPLLDLTHFLASYGFPITVLVTPKNLPILQPLLSAHP 60

Query: 61  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 120
            VQTLVLPFPSVP LPPGVEN+KDIGG+GNVPI+VALRQLQDPI+EWFKSHPSPPSAII+
Sbjct: 61  SVQTLVLPFPSVPGLPPGVENIKDIGGEGNVPIIVALRQLQDPIIEWFKSHPSPPSAIIS 120

Query: 121 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 180
           DFFLGWT+SLA+KLQIPR++F+SSG WVV++LDYCWR+V SE+FRKSPVKHMAELPNSP+
Sbjct: 121 DFFLGWTQSLAQKLQIPRVSFFSSGCWVVDVLDYCWRHVPSEEFRKSPVKHMAELPNSPS 180

Query: 181 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGG-S 240
           FTNQDLPEFVT+Y DSDP +  IR+DW E  TCWA VFNTF+ LE +YL+++RKLN   +
Sbjct: 181 FTNQDLPEFVTVYRDSDPLFSTIRADWFEAGTCWANVFNTFDELEPEYLEFYRKLNNNEN 240

Query: 241 VFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 300
           VFGVGPLSL+  H+P  +SSSDE+LKWLD CPD SVLY+SFGSQKQLNQQQMEALASG+E
Sbjct: 241 VFGVGPLSLVKGHNPTVESSSDEVLKWLDECPDGSVLYISFGSQKQLNQQQMEALASGIE 300

Query: 301 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 360
           KSG RF+WVVKTI QT G SDGIPIGFEDRV  RG+VV+RW PQ AIL HRAV GFLSHC
Sbjct: 301 KSGTRFVWVVKTIRQTDGGSDGIPIGFEDRVSGRGMVVKRWVPQEAILRHRAVRGFLSHC 360

Query: 361 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 420
           GWNS+LESL SGVPIFAWAMEGDQM+NSK+LVD +GVAVQVCHGENSVPD D+LG+V+AE
Sbjct: 361 GWNSILESLASGVPIFAWAMEGDQMINSKVLVDQIGVAVQVCHGENSVPDPDKLGEVLAE 420

Query: 421 SFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPS 466
           SFNSD+LKAKA+ALSKAV DA GP+GSS+R LQEFA KLA+LPPP+
Sbjct: 421 SFNSDKLKAKARALSKAVADATGPSGSSLRNLQEFAKKLASLPPPT 465

BLAST of Lsi04G003390 vs. ExPASy TrEMBL
Match: A0A6J1IRU4 (Glycosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111477873 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.6e-224
Identity = 377/466 (80.90%), Postives = 419/466 (89.91%), Query Frame = 0

Query: 1   MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP 60
           MAS PSSPLPHLL+FPYPAQGHMLPLLDLTHFLASYGF ITVLVTPKNLPILQPLLSAHP
Sbjct: 1   MAS-PSSPLPHLLLFPYPAQGHMLPLLDLTHFLASYGFPITVLVTPKNLPILQPLLSAHP 60

Query: 61  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 120
            VQTLVLPFPSVP LPPGVEN+KDIG +GNVPI+VALRQLQDPIV WFKSHPSPPSAII+
Sbjct: 61  AVQTLVLPFPSVPGLPPGVENIKDIGNEGNVPIIVALRQLQDPIVAWFKSHPSPPSAIIS 120

Query: 121 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 180
           DFFLGWT+SLA+KLQIPR++F+SSG WVV++LDYCWR+V SE+FRKSPVKHMAELPNSP+
Sbjct: 121 DFFLGWTQSLAQKLQIPRVSFFSSGCWVVDVLDYCWRHVPSEEFRKSPVKHMAELPNSPS 180

Query: 181 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGG-S 240
           FTNQDLPEF T Y DSDP +  IR+DW E  TCWA VFNTF+ LE +YL+++RKLN   +
Sbjct: 181 FTNQDLPEFATAYRDSDPLFSTIRADWFEARTCWAHVFNTFDELEPEYLEFYRKLNNNKN 240

Query: 241 VFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 300
           VFGVGPLSL+  H+P  +SSSDE+LKWLD  PD SVLY+SFGSQKQLNQQQMEALASG+E
Sbjct: 241 VFGVGPLSLVKGHNPTVESSSDEVLKWLDERPDGSVLYISFGSQKQLNQQQMEALASGIE 300

Query: 301 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 360
           KSG RF+WVVKTI QT G SDGIPIGFEDRV  RG+VV+RW PQ AIL HRAV GFLSHC
Sbjct: 301 KSGTRFVWVVKTIRQTDGGSDGIPIGFEDRVSGRGMVVKRWVPQEAILRHRAVRGFLSHC 360

Query: 361 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 420
           GWNS+LESL SGVPIFAWAMEGDQMMNSKILVD +GVAVQVCHG+NSVPD DQLG+V+AE
Sbjct: 361 GWNSILESLASGVPIFAWAMEGDQMMNSKILVDQIGVAVQVCHGDNSVPDPDQLGEVLAE 420

Query: 421 SFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPS 466
           SFNSD+LKAKA+ALSKAV DA GPNGSS+R LQEFA KLA+LPPP+
Sbjct: 421 SFNSDKLKAKARALSKAVADATGPNGSSLRNLQEFAKKLASLPPPT 465

BLAST of Lsi04G003390 vs. ExPASy TrEMBL
Match: A0A6J1FZE5 (UDP-glycosyltransferase 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111449303 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 2.2e-170
Identity = 301/464 (64.87%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 5   PSSPLPHLLVFPYPAQGHMLPLLDLTHFLASY-GFTITVLVTPKNLPILQPLLSAHPLVQ 64
           PS+  PHLLVFPYPAQGHM+ LLDLTH+LAS+ GFTITVLVTPKNLP+L+PLL AHP + 
Sbjct: 10  PSTTTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLEPLLQAHPSIH 69

Query: 65  TLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFF 124
           TLVLPFP+ P+LP G E+V  IG  GN PIMVALRQL DPIV WF SHP+PP+AII+D F
Sbjct: 70  TLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAAIISDSF 129

Query: 125 LGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTN 184
           LGWT+ LA  LQIPRI +YS GA++VN+L Y W +++++ FR SPV H  E+PNSP+F  
Sbjct: 130 LGWTQHLADHLQIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPNSPSFKK 189

Query: 185 QDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSVFG 244
             LP  +  Y+DS+P W  +R D L  ++ W  VFN+FE LE ++L++FR LNG G VFG
Sbjct: 190 DHLPSLIKRYQDSNPDWDLLRDDALANTSGWVSVFNSFETLEGEFLEHFRDLNGEGRVFG 249

Query: 245 VGPLSLIHS---HSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 304
           +GPL+LI +    S + DSSS E+L WLDGC D SVLYV FGSQKQLNQQQMEALASGLE
Sbjct: 250 IGPLNLIRAGDGRSSIRDSSS-EVLTWLDGCADSSVLYVCFGSQKQLNQQQMEALASGLE 309

Query: 305 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 364
           +SG RF+WVVKT +QTVG  +GIPIGFEDRV DRGIVV+ W PQVAILNHRAVGGFLSHC
Sbjct: 310 RSGARFVWVVKTFNQTVGGCNGIPIGFEDRVSDRGIVVKEWVPQVAILNHRAVGGFLSHC 369

Query: 365 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 424
           GWNSVLES+ +GV I  W ME DQ +N+K+LVD +GVAV+VC G NSVPDSD+LGKVIA 
Sbjct: 370 GWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDELGKVIAM 429

Query: 425 SFNSD-ELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLP 463
           S   D   K KA AL +A VDA   NGSS + ++ F +KL  LP
Sbjct: 430 SLRGDCPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLKQLP 472

BLAST of Lsi04G003390 vs. ExPASy TrEMBL
Match: A0A346A6C1 (Flavonoids UDP-glycosyltransferase OS=Siraitia grosvenorii OX=190515 GN=UGT89M1 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 5.4e-169
Identity = 303/464 (65.30%), Postives = 355/464 (76.51%), Query Frame = 0

Query: 5   PSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHPLVQT 64
           PS+   HLLVFPYPAQGHMLPLLDLT  LASYGFTITVLVTPKNL +LQPLLS HP V T
Sbjct: 10  PSTSSRHLLVFPYPAQGHMLPLLDLTRRLASYGFTITVLVTPKNLSLLQPLLSTHPSVHT 69

Query: 65  LVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFL 124
           LVLPFP  P LPPGVE+V+DIG  GN PIM+ALR+L  PI+ WF SHPSPP AII+DFFL
Sbjct: 70  LVLPFPPHPKLPPGVEHVRDIGNLGNFPIMLALRELHGPILHWFDSHPSPPVAIISDFFL 129

Query: 125 GWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTNQ 184
           GWT+ LA  L IPRI FYSSGA+ VN++DYCW +++++ FR SPV  +  +P SP+F  +
Sbjct: 130 GWTQRLADDLGIPRIVFYSSGAFFVNVVDYCWVHIKTDYFRSSPVIEICSIPKSPSFKKE 189

Query: 185 DLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSVFGV 244
            LP  V  Y+DSDP    +R   +   + WA +FNTFE LE ++L++ RK+ G GSVFGV
Sbjct: 190 HLPSMVRAYKDSDPDSDLLRDSAMINKSSWATIFNTFEKLEHEHLEHLRKVRGEGSVFGV 249

Query: 245 GPLSLI---HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEK 304
           GPL LI      +P+ D SSD +LKWLDGCPD SVLYV FGSQKQLNQQQ EALASGLEK
Sbjct: 250 GPLCLIGAEDGRNPIRDLSSD-VLKWLDGCPDGSVLYVCFGSQKQLNQQQTEALASGLEK 309

Query: 305 SGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCG 364
           SG RF+WVVKTI QT G SDGIPIGFEDRV DRG VV+ WAPQV ILNH+AVGGFLSHCG
Sbjct: 310 SGARFVWVVKTI-QTEGGSDGIPIGFEDRVSDRGFVVKEWAPQVLILNHQAVGGFLSHCG 369

Query: 365 WNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAES 424
           WNSVLES+V+GV I  W ME DQ +N+++LVD +GV+V+VC G NSVPD D+LGKVIA+S
Sbjct: 370 WNSVLESIVAGVMILGWPMEADQFINARLLVDDMGVSVRVCEGANSVPDPDELGKVIAKS 429

Query: 425 FNSDE-LKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPP 464
              +   K KAKAL +  VDA G NGSS   + +  NKL  LPP
Sbjct: 430 LRGESPEKMKAKALRREAVDAMGTNGSSWNDMHDLVNKLNELPP 471

BLAST of Lsi04G003390 vs. ExPASy TrEMBL
Match: A0A6J1HY91 (UDP-glycosyltransferase 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111467348 PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 9.1e-169
Identity = 300/467 (64.24%), Postives = 362/467 (77.52%), Query Frame = 0

Query: 2   ASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASY-GFTITVLVTPKNLPILQPLLSAHP 61
           +S PS+  PHLLVFPYPAQGHM+ LLDLTH+LAS+ GFTITVLVTPKNLP+LQPLL AHP
Sbjct: 3   SSPPSTTTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLQPLLQAHP 62

Query: 62  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 121
            + TLVLPFP+ P+LP G E+V  IG  GN PIMVALRQL DPIV WF SHP+PP+AII+
Sbjct: 63  SIHTLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAAIIS 122

Query: 122 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 181
           DFFLGWT+ LA  L+IPRI +YS GA++VN+L Y W +++++ FR SPV H  E+PNSP+
Sbjct: 123 DFFLGWTQHLADHLRIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPNSPS 182

Query: 182 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GS 241
           F    LP  +  Y+DS+  W  +R+D L  ++ W  VFN+FE LE ++L++FR LNG G 
Sbjct: 183 FKKDHLPSLIKRYQDSNLDWDLLRNDALANTSGWVSVFNSFETLEGEFLEHFRDLNGEGR 242

Query: 242 VFGVGPLSLI---HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALAS 301
           VFG+GPL+LI      S + DSSS E+L WLDGC D SVLYV FGSQKQLNQQQM+ALAS
Sbjct: 243 VFGIGPLNLIGAGDGRSSIRDSSS-EVLTWLDGCADSSVLYVCFGSQKQLNQQQMKALAS 302

Query: 302 GLEKSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFL 361
           GLEKSG RF+WVVKT +QTVG  + IPIGFEDRV DRGIVV+ W PQVAILNH+AVGGFL
Sbjct: 303 GLEKSGARFVWVVKTFNQTVGGCNEIPIGFEDRVSDRGIVVKEWVPQVAILNHQAVGGFL 362

Query: 362 SHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKV 421
           SHCGWNSVLES+ +GV I  W ME DQ +N+K+LVD +GVAV+VC G NSVPDSD+LGKV
Sbjct: 363 SHCGWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDELGKV 422

Query: 422 IAESFNSDE-LKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLP 463
           IA S + D   K KA AL +A VDA   NGSS + ++ F +KL  LP
Sbjct: 423 IAMSLSGDRPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLNQLP 468

BLAST of Lsi04G003390 vs. NCBI nr
Match: XP_022956873.1 (UDP-glycosyltransferase 89A2-like [Cucurbita moschata] >XP_023536862.1 UDP-glycosyltransferase 89A2-like [Cucurbita pepo subsp. pepo] >KAG7032141.1 UDP-glycosyltransferase 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796.2 bits (2055), Expect = 1.5e-226
Identity = 377/466 (80.90%), Postives = 424/466 (90.99%), Query Frame = 0

Query: 1   MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP 60
           MAS PSSPLPHLL+FPYPAQGHMLPLLDLTHFLASYGF ITVLVTPKNLPILQPLLSAHP
Sbjct: 1   MAS-PSSPLPHLLLFPYPAQGHMLPLLDLTHFLASYGFPITVLVTPKNLPILQPLLSAHP 60

Query: 61  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 120
            VQTLVLPFPSVP LPPGVEN+KDIGG+GNVPI+VALRQLQDPI+EWFKSHPSPPSAII+
Sbjct: 61  SVQTLVLPFPSVPGLPPGVENIKDIGGEGNVPIIVALRQLQDPIIEWFKSHPSPPSAIIS 120

Query: 121 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 180
           DFFLGWT+SLA+KLQIPR++F+SSG WVV++LDYCWR+V SE+FRKSPVKHMAELPNSP+
Sbjct: 121 DFFLGWTQSLAQKLQIPRVSFFSSGCWVVDVLDYCWRHVPSEEFRKSPVKHMAELPNSPS 180

Query: 181 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGG-S 240
           FTNQDLPEFVT+Y DSDP +  IR+DW E  TCWA VFNTF+ LE +YL+++RKLN   +
Sbjct: 181 FTNQDLPEFVTVYRDSDPLFSTIRADWFEAGTCWANVFNTFDELEPEYLEFYRKLNNNEN 240

Query: 241 VFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 300
           VFGVGPLSL+  H+P  +SSSDE+LKWLD CPD SVLY+SFGSQKQLNQQQMEALASG+E
Sbjct: 241 VFGVGPLSLVKGHNPTVESSSDEVLKWLDECPDGSVLYISFGSQKQLNQQQMEALASGIE 300

Query: 301 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 360
           KSG RF+WVVKTI QT G SDGIPIGFEDRV  RG+VV+RW PQ AIL HRAV GFLSHC
Sbjct: 301 KSGTRFVWVVKTIRQTDGGSDGIPIGFEDRVSGRGMVVKRWVPQEAILRHRAVRGFLSHC 360

Query: 361 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 420
           GWNS+LESL SGVPIFAWAMEGDQM+NSK+LVD +GVAVQVCHGENSVPD D+LG+V+AE
Sbjct: 361 GWNSILESLASGVPIFAWAMEGDQMINSKVLVDQIGVAVQVCHGENSVPDPDKLGEVLAE 420

Query: 421 SFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPS 466
           SFNSD+LKAKA+ALSKAV DA GP+GSS+R LQEFA KLA+LPPP+
Sbjct: 421 SFNSDKLKAKARALSKAVADATGPSGSSLRNLQEFAKKLASLPPPT 465

BLAST of Lsi04G003390 vs. NCBI nr
Match: KAG6601355.1 (UDP-glycosyltransferase 89A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793.5 bits (2048), Expect = 9.9e-226
Identity = 376/466 (80.69%), Postives = 423/466 (90.77%), Query Frame = 0

Query: 1   MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP 60
           MAS PSSPLPHLL+FPYPAQGHMLPLLDLTHFLASYGF ITVLVTPKNLPILQPLLSAHP
Sbjct: 1   MAS-PSSPLPHLLLFPYPAQGHMLPLLDLTHFLASYGFPITVLVTPKNLPILQPLLSAHP 60

Query: 61  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 120
            VQTLVLPFPSVP LPPGVEN+KDIGG+GNVPI+VALRQLQDPI+EWFKSHPSPPSAII+
Sbjct: 61  SVQTLVLPFPSVPGLPPGVENIKDIGGEGNVPIIVALRQLQDPIIEWFKSHPSPPSAIIS 120

Query: 121 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 180
           DFFLGWT+SLA+KLQIPR++F+SSG WVV++LDYCWR+V SE+FRKSPVKHMAELPNSP+
Sbjct: 121 DFFLGWTQSLAQKLQIPRVSFFSSGCWVVDVLDYCWRHVPSEEFRKSPVKHMAELPNSPS 180

Query: 181 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGG-S 240
           FTNQDLPEFVT+Y DSDP +  IR+DW E  TCWA VFNTF+ LE +YL+++RKLN   +
Sbjct: 181 FTNQDLPEFVTVYRDSDPLFSTIRADWFEAGTCWANVFNTFDELEPEYLEFYRKLNNNEN 240

Query: 241 VFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 300
           VFGVGPLSL+  H+P  +SSSDE+LKWLD CP  SVLY+SFGSQKQLNQQQMEALASG+E
Sbjct: 241 VFGVGPLSLVKGHNPTVESSSDEVLKWLDECPHGSVLYISFGSQKQLNQQQMEALASGIE 300

Query: 301 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 360
           KSG RF+WVVKTI QT G SDGIPIGFEDRV  RG+VV+RW PQ AIL HRAV GFLSHC
Sbjct: 301 KSGTRFVWVVKTIRQTDGGSDGIPIGFEDRVSGRGMVVKRWVPQEAILRHRAVRGFLSHC 360

Query: 361 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 420
           GWNS+LESL SGVPIFAWAMEGDQM+NSK+LVD +GVAVQVCHGENSVPD D+LG+V+AE
Sbjct: 361 GWNSILESLASGVPIFAWAMEGDQMINSKVLVDQIGVAVQVCHGENSVPDPDKLGEVLAE 420

Query: 421 SFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPS 466
           SFNSD+LKAKA+ALSKAV DA GP+GSS+R LQEFA KLA+LPPP+
Sbjct: 421 SFNSDKLKAKARALSKAVADATGPSGSSLRNLQEFAKKLASLPPPT 465

BLAST of Lsi04G003390 vs. NCBI nr
Match: XP_022977594.1 (UDP-glycosyltransferase 89A2-like [Cucurbita maxima])

HSP 1 Score: 787.7 bits (2033), Expect = 5.4e-224
Identity = 377/466 (80.90%), Postives = 419/466 (89.91%), Query Frame = 0

Query: 1   MASLPSSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP 60
           MAS PSSPLPHLL+FPYPAQGHMLPLLDLTHFLASYGF ITVLVTPKNLPILQPLLSAHP
Sbjct: 1   MAS-PSSPLPHLLLFPYPAQGHMLPLLDLTHFLASYGFPITVLVTPKNLPILQPLLSAHP 60

Query: 61  LVQTLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIA 120
            VQTLVLPFPSVP LPPGVEN+KDIG +GNVPI+VALRQLQDPIV WFKSHPSPPSAII+
Sbjct: 61  AVQTLVLPFPSVPGLPPGVENIKDIGNEGNVPIIVALRQLQDPIVAWFKSHPSPPSAIIS 120

Query: 121 DFFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPT 180
           DFFLGWT+SLA+KLQIPR++F+SSG WVV++LDYCWR+V SE+FRKSPVKHMAELPNSP+
Sbjct: 121 DFFLGWTQSLAQKLQIPRVSFFSSGCWVVDVLDYCWRHVPSEEFRKSPVKHMAELPNSPS 180

Query: 181 FTNQDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNGG-S 240
           FTNQDLPEF T Y DSDP +  IR+DW E  TCWA VFNTF+ LE +YL+++RKLN   +
Sbjct: 181 FTNQDLPEFATAYRDSDPLFSTIRADWFEARTCWAHVFNTFDELEPEYLEFYRKLNNNKN 240

Query: 241 VFGVGPLSLIHSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 300
           VFGVGPLSL+  H+P  +SSSDE+LKWLD  PD SVLY+SFGSQKQLNQQQMEALASG+E
Sbjct: 241 VFGVGPLSLVKGHNPTVESSSDEVLKWLDERPDGSVLYISFGSQKQLNQQQMEALASGIE 300

Query: 301 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 360
           KSG RF+WVVKTI QT G SDGIPIGFEDRV  RG+VV+RW PQ AIL HRAV GFLSHC
Sbjct: 301 KSGTRFVWVVKTIRQTDGGSDGIPIGFEDRVSGRGMVVKRWVPQEAILRHRAVRGFLSHC 360

Query: 361 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 420
           GWNS+LESL SGVPIFAWAMEGDQMMNSKILVD +GVAVQVCHG+NSVPD DQLG+V+AE
Sbjct: 361 GWNSILESLASGVPIFAWAMEGDQMMNSKILVDQIGVAVQVCHGDNSVPDPDQLGEVLAE 420

Query: 421 SFNSDELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLPPPS 466
           SFNSD+LKAKA+ALSKAV DA GPNGSS+R LQEFA KLA+LPPP+
Sbjct: 421 SFNSDKLKAKARALSKAVADATGPNGSSLRNLQEFAKKLASLPPPT 465

BLAST of Lsi04G003390 vs. NCBI nr
Match: XP_022944915.1 (UDP-glycosyltransferase 89A2-like [Cucurbita moschata])

HSP 1 Score: 608.6 bits (1568), Expect = 4.5e-170
Identity = 301/464 (64.87%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 5   PSSPLPHLLVFPYPAQGHMLPLLDLTHFLASY-GFTITVLVTPKNLPILQPLLSAHPLVQ 64
           PS+  PHLLVFPYPAQGHM+ LLDLTH+LAS+ GFTITVLVTPKNLP+L+PLL AHP + 
Sbjct: 10  PSTTTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLEPLLQAHPSIH 69

Query: 65  TLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFF 124
           TLVLPFP+ P+LP G E+V  IG  GN PIMVALRQL DPIV WF SHP+PP+AII+D F
Sbjct: 70  TLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAAIISDSF 129

Query: 125 LGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTN 184
           LGWT+ LA  LQIPRI +YS GA++VN+L Y W +++++ FR SPV H  E+PNSP+F  
Sbjct: 130 LGWTQHLADHLQIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPNSPSFKK 189

Query: 185 QDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSVFG 244
             LP  +  Y+DS+P W  +R D L  ++ W  VFN+FE LE ++L++FR LNG G VFG
Sbjct: 190 DHLPSLIKRYQDSNPDWDLLRDDALANTSGWVSVFNSFETLEGEFLEHFRDLNGEGRVFG 249

Query: 245 VGPLSLIHS---HSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 304
           +GPL+LI +    S + DSSS E+L WLDGC D SVLYV FGSQKQLNQQQMEALASGLE
Sbjct: 250 IGPLNLIRAGDGRSSIRDSSS-EVLTWLDGCADSSVLYVCFGSQKQLNQQQMEALASGLE 309

Query: 305 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 364
           +SG RF+WVVKT +QTVG  +GIPIGFEDRV DRGIVV+ W PQVAILNHRAVGGFLSHC
Sbjct: 310 RSGARFVWVVKTFNQTVGGCNGIPIGFEDRVSDRGIVVKEWVPQVAILNHRAVGGFLSHC 369

Query: 365 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 424
           GWNSVLES+ +GV I  W ME DQ +N+K+LVD +GVAV+VC G NSVPDSD+LGKVIA 
Sbjct: 370 GWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDELGKVIAM 429

Query: 425 SFNSD-ELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLP 463
           S   D   K KA AL +A VDA   NGSS + ++ F +KL  LP
Sbjct: 430 SLRGDCPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLKQLP 472

BLAST of Lsi04G003390 vs. NCBI nr
Match: XP_023542842.1 (UDP-glycosyltransferase 89A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.2 bits (1567), Expect = 5.9e-170
Identity = 299/464 (64.44%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 5   PSSPLPHLLVFPYPAQGHMLPLLDLTHFLASY-GFTITVLVTPKNLPILQPLLSAHPLVQ 64
           PS+  PHLLVFPYPAQGHM+ LLDLTH+LAS+ GFTITVL+TPKNLP+LQPLL AHP + 
Sbjct: 10  PSTTTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLITPKNLPLLQPLLQAHPSIH 69

Query: 65  TLVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFF 124
           TLVLPFP+ P+LP G E+V  IG  GN PIMVALRQL DPIV WF SHP+PP+AII+D F
Sbjct: 70  TLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAAIISDSF 129

Query: 125 LGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTN 184
           LGWT+ LA  LQIPRI +YS GA++VN+L Y W +++++ FR SPV H  E+PNSP+F  
Sbjct: 130 LGWTQHLADHLQIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPNSPSFKK 189

Query: 185 QDLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSVFG 244
             LP  +  Y+DS+P W  +R D L  ++CW  VFN+FE LE ++L++FR LNG G VFG
Sbjct: 190 DHLPSLIKRYQDSNPDWDLLRDDALANTSCWVSVFNSFETLEGEFLEHFRDLNGEGRVFG 249

Query: 245 VGPLSLI---HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 304
           +GPL+LI      S + DS+S E+L WLDGC D SVLYV FGSQKQLNQQQMEALASGLE
Sbjct: 250 IGPLNLIGAADGRSSIRDSTS-EVLTWLDGCADSSVLYVCFGSQKQLNQQQMEALASGLE 309

Query: 305 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 364
           +SG RF+WVVKT +QTVG  + IPIGFEDRV DRGIVV+ W PQVAILNHRAVGGFLSHC
Sbjct: 310 RSGARFVWVVKTFNQTVGGCNEIPIGFEDRVSDRGIVVKEWVPQVAILNHRAVGGFLSHC 369

Query: 365 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 424
           GWNSVLES+ +GV I  W ME DQ +N+K+LVD +GVAV+VC G NSVPDSD+LGKVIA 
Sbjct: 370 GWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDELGKVIAM 429

Query: 425 SFNSD-ELKAKAKALSKAVVDAAGPNGSSVRALQEFANKLATLP 463
           S + D   K KA AL +A VD+   NGSS + ++ F +KL  LP
Sbjct: 430 SLSGDCPEKMKAGALKRAAVDSVRSNGSSWKDMRGFVDKLKQLP 472

BLAST of Lsi04G003390 vs. TAIR 10
Match: AT5G03490.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 7.8e-120
Identity = 231/464 (49.78%), Postives = 304/464 (65.52%), Query Frame = 0

Query: 6   SSPLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP-LVQT 65
           +S  PH++VFP+PAQGH+LPLLDLTH L   GF ++V+VTP NL  L PLLSAHP  V +
Sbjct: 14  NSKPPHIVVFPFPAQGHLLPLLDLTHQLCLRGFNVSVIVTPGNLTYLSPLLSAHPSSVTS 73

Query: 66  LVLPFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFL 125
           +V PFP  P L PGVENVKD+G  GN+PIM +LRQL++PI+ WF+SHP+PP A+I+DFFL
Sbjct: 74  VVFPFPPHPSLSPGVENVKDVGNSGNLPIMASLRQLREPIINWFQSHPNPPIALISDFFL 133

Query: 126 GWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKS--PVKHMAELPNSPTFT 185
           GWT  L  ++ IPR  F+S   ++V++L +C+  +   D  KS  P+ H+ +LP +P F 
Sbjct: 134 GWTHDLCNQIGIPRFAFFSISFFLVSVLQFCFENI---DLIKSTDPI-HLLDLPRAPIFK 193

Query: 186 NQDLPEFV-TIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYFRKLNG-GSV 245
            + LP  V    +   P   +I+ D+      +  VFN+ E LE DYL Y ++  G   V
Sbjct: 194 EEHLPSIVRRSLQTPSPDLESIK-DFSMNLLSYGSVFNSSEILEDDYLQYVKQRMGHDRV 253

Query: 246 FGVGPLSLIHSHSPLADSSSD-EILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLE 305
           + +GPL  I S       S D  +L WLDG P+ SVLYV FGSQK L + Q +ALA GLE
Sbjct: 254 YVIGPLCSIGSGLKSNSGSVDPSLLSWLDGSPNGSVLYVCFGSQKALTKDQCDALALGLE 313

Query: 306 KSGIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHC 365
           KS  RF+WVVK         D IP GFEDRV  RG+VVR W  Q+A+L H AVGGFLSHC
Sbjct: 314 KSMTRFVWVVK--------KDPIPDGFEDRVSGRGLVVRGWVSQLAVLRHVAVGGFLSHC 373

Query: 366 GWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAE 425
           GWNSVLE + SG  I  W ME DQ +N+++LV+H+GVAV+VC G  +VPDSD+LG+VIAE
Sbjct: 374 GWNSVLEGITSGAVILGWPMEADQFVNARLLVEHLGVAVRVCEGGETVPDSDELGRVIAE 433

Query: 426 SFNSD--ELKAKAKALSK----AVVDAAGPNGSSVRALQEFANK 458
           +      E+ A+A+ + +    AV +A   NGSSV  +Q    +
Sbjct: 434 TMGEGGREVAARAEEIRRKTEAAVTEA---NGSSVENVQRLVKE 461

BLAST of Lsi04G003390 vs. TAIR 10
Match: AT1G51210.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.2e-117
Identity = 215/429 (50.12%), Postives = 289/429 (67.37%), Query Frame = 0

Query: 10  PHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAHP-LVQTLVLP 69
           PH++VFPYPAQGH+LPLLDLTH L   G T++++VTPKNLP L PLLSAHP  V  + LP
Sbjct: 19  PHIMVFPYPAQGHLLPLLDLTHQLCLRGLTVSIIVTPKNLPYLSPLLSAHPSAVSVVTLP 78

Query: 70  FPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIADFFLGWTE 129
           FP  P +P GVENVKD+GG GN  IM +LRQL++PIV W  SHP+PP A+I+DFFLGWT+
Sbjct: 79  FPHHPLIPSGVENVKDLGGYGNPLIMASLRQLREPIVNWLSSHPNPPVALISDFFLGWTK 138

Query: 130 SLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTNQDLPE 189
            L     IPR  F+SSGA++ +IL +     +   F  +    +++LP SP F  + LP 
Sbjct: 139 DLG----IPRFAFFSSGAFLASILHFV--SDKPHLFESTEPVCLSDLPRSPVFKTEHLPS 198

Query: 190 FVTIYEDSDPRWHAIRS--DWLETSTCWAGVFNTFEALEQDYLDYFR-KLNGGSVFGVGP 249
            +       P    + S  D     + +  +FNT E LE+DY++Y + K++   VFGVGP
Sbjct: 199 LI----PQSPLSQDLESVKDSTMNFSSYGCIFNTCECLEEDYMEYVKQKVSENRVFGVGP 258

Query: 250 LSLI--HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEKSGI 309
           LS +       +++  +  +L WLDGCPDDSVLY+ FGSQK L ++Q + LA GLEKS  
Sbjct: 259 LSSVGLSKEDSVSNVDAKALLSWLDGCPDDSVLYICFGSQKVLTKEQCDDLALGLEKSMT 318

Query: 310 RFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCGWNS 369
           RF+WVVK         D IP GFEDRV  RG++VR WAPQVA+L+H AVGGFL HCGWNS
Sbjct: 319 RFVWVVK--------KDPIPDGFEDRVAGRGMIVRGWAPQVAMLSHVAVGGFLIHCGWNS 378

Query: 370 VLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAESFNS 429
           VLE++ SG  I AW ME DQ ++++++V+H+GVAV VC G  +VPD  ++G++IA++   
Sbjct: 379 VLEAMASGTMILAWPMEADQFVDARLVVEHMGVAVSVCEGGKTVPDPYEMGRIIADTMGE 429

Query: 430 DELKAKAKA 433
              +A+A+A
Sbjct: 439 SGGEARARA 429

BLAST of Lsi04G003390 vs. TAIR 10
Match: AT1G73880.1 (UDP-glucosyl transferase 89B1 )

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-104
Identity = 202/461 (43.82%), Postives = 279/461 (60.52%), Query Frame = 0

Query: 11  HLLVFPYPAQGHMLPLLDLTHFLASYG---FTITVLVTPKNLPILQPLLSAHPLVQTLVL 70
           H+L+FP+PAQGHM+PLLD TH LA  G     ITVLVTPKNLP L PLLSA   ++ L+L
Sbjct: 14  HVLIFPFPAQGHMIPLLDFTHRLALRGGAALKITVLVTPKNLPFLSPLLSAVVNIEPLIL 73

Query: 71  PFPSVPDLPPGVENVKDIGGQGNVPIMV-ALRQLQDPIVEWFKSHPSPPSAIIADFFLGW 130
           PFPS P +P GVENV+D+   G  P+M+ AL  L  P++ W  SHPSPP AI++DFFLGW
Sbjct: 74  PFPSHPSIPSGVENVQDLPPSG-FPLMIHALGNLHAPLISWITSHPSPPVAIVSDFFLGW 133

Query: 131 TESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSE--DFRKSPVKHMAELPNSPTFTNQ 190
           T++L     IPR  F  S A    IL+  W  + ++  +   + + H  ++PN P +   
Sbjct: 134 TKNLG----IPRFDFSPSAAITCCILNTLWIEMPTKINEDDDNEILHFPKIPNCPKYRFD 193

Query: 191 DLPEFVTIYEDSDPRWHAIRSDWLETSTCWAGVFNTFEALEQDYLDYF-RKLNGGSVFGV 250
            +      Y   DP W  IR  + +    W  V N+F A+E  YL++  R++    V+ V
Sbjct: 194 QISSLYRSYVHGDPAWEFIRDSFRDNVASWGLVVNSFTAMEGVYLEHLKREMGHDRVWAV 253

Query: 251 GPLSLI--HSHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQMEALASGLEKS 310
           GP+  +   +       S D ++ WLD   D+ V+YV FGSQ  L ++Q  ALASGLEKS
Sbjct: 254 GPIIPLSGDNRGGPTSVSVDHVMSWLDAREDNHVVYVCFGSQVVLTKEQTLALASGLEKS 313

Query: 311 GIRFLWVVKTIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVAILNHRAVGGFLSHCGW 370
           G+ F+W VK   +       I  GF+DRV  RG+V+R WAPQVA+L HRAVG FL+HCGW
Sbjct: 314 GVHFIWAVKEPVEKDSTRGNILDGFDDRVAGRGLVIRGWAPQVAVLRHRAVGAFLTHCGW 373

Query: 371 NSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDSDQLGKVIAESF 430
           NSV+E++V+GV +  W M  DQ  ++ ++VD + V V+ C G ++VPD D+L +V A+S 
Sbjct: 374 NSVVEAVVAGVLMLTWPMRADQYTDASLVVDELKVGVRACEGPDTVPDPDELARVFADSV 433

Query: 431 NSDEL-KAKAKALSKAVVDAAGPNGSSVRALQEFANKLATL 462
             ++  + KA  L KA +DA    GSSV  L  F   + +L
Sbjct: 434 TGNQTERIKAVELRKAALDAIQERGSSVNDLDGFIQHVVSL 469

BLAST of Lsi04G003390 vs. TAIR 10
Match: AT1G06000.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 6.0e-72
Identity = 173/469 (36.89%), Postives = 247/469 (52.67%), Query Frame = 0

Query: 10  PHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNLPILQPLLSAH--PLVQTLVL 69
           PH+LV P+P  GHM+P LDLTH +   G T+TVLVTPKN   L  L S H     +TL+L
Sbjct: 9   PHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLIL 68

Query: 70  PFPSVPDLPPGVENVKDIGGQGNVPIMVALRQLQDPIVEWFKSHPSP--PSAIIADFFLG 129
           PFPS P +P GVE+++ +  +  V +  AL +L DP+V++    P    P AI+   FL 
Sbjct: 69  PFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLS 128

Query: 130 -WTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKHMAELPNSPTFTNQ 189
            W   +A    I  I+F    A  ++++   W     ED                +F N 
Sbjct: 129 PWINKVADAFSIKSISFLPINAHSISVM---W---AQED---------------RSFFND 188

Query: 190 DLPEFVTIYEDSDPRWHAIRSDWLETST--CWAGVFNTFEALEQDYLDYF--RKLNGGSV 249
                                  LET+T   +  V N+F  LE ++++    R LN   +
Sbjct: 189 -----------------------LETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRI 248

Query: 250 FGVGPLSLIHSHSPLADSSS---DEILKWLDGCPDD-SVLYVSFGSQKQLNQQQMEALAS 309
           + VGPL    +       SS    ++  WLD CP+D SV+YV FGSQ +L  +Q  ALA+
Sbjct: 249 WTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAA 308

Query: 310 GLEKSGIRFLWVVKTIHQTVGESDG------IPIGFEDRVLDRGIVVRRWAPQVAILNHR 369
            LEKS +RF+W V+   + V  SD       IP GFE+RV ++G+V+R WAPQ  IL HR
Sbjct: 309 ALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHR 368

Query: 370 AVGGFLSHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVDHVGVAVQVCHGENSVPDS 429
           AVG +L+H GW SVLE +V GV + AW M+ D   N+ ++VD +  AV+V    +SVPDS
Sbjct: 369 AVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDS 428

Query: 430 DQLGKVIAESFNSD-ELKAKAKALSKAVVDAAGPNGSSVRALQEFANKL 459
           D+L +++AES   D   +     L +  ++A    GSS + L E   ++
Sbjct: 429 DKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEM 433

BLAST of Lsi04G003390 vs. TAIR 10
Match: AT2G36770.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 5.7e-62
Identity = 152/476 (31.93%), Postives = 240/476 (50.42%), Query Frame = 0

Query: 8   PLPHLLVFPYPAQGHMLPLLDLTHFLASYGFTITVLVTPKNL----PILQPLLSAHPLVQ 67
           P  H ++FP+ AQGHM+P++D+   LA  G T+T++ T  N      +L   + +   + 
Sbjct: 11  PPLHFILFPFMAQGHMIPMIDIARLLAQRGATVTIVTTRYNAGRFENVLSRAMESGLPIN 70

Query: 68  TLVLPFPSVP-DLPPGVENVKDIGG-QGNVPIMVALRQLQDPIVEWFKSHPSPPSAIIAD 127
            + + FP     LP G EN+      +  VP   A+  L+DP+++  +     PS II+D
Sbjct: 71  IVHVNFPYQEFGLPEGKENIDSYDSMELMVPFFQAVNMLEDPVMKLMEEMKPRPSCIISD 130

Query: 128 FFLGWTESLARKLQIPRITFYSSGAWVVNILDYCWRYVQSEDFRKSPVKH--MAELPNSP 187
             L +T  +ARK  IP+I F+ +G + +  +    R ++     KS   +  +   P+  
Sbjct: 131 LLLPYTSKIARKFSIPKIVFHGTGCFNLLCMHVLRRNLEILKNLKSDKDYFLVPSFPDRV 190

Query: 188 TFTNQDLPEFVTIYEDSDPRWHAIRSDWLETS-TCWAGVFNTFEALEQDYLDYFRKLNGG 247
            FT   +P   T   D    W A   + +E   T +  + NTF+ LE  Y+  + K   G
Sbjct: 191 EFTKPQVPVETTASGD----WKAFLDEMVEAEYTSYGVIVNTFQELEPAYVKDYTKARAG 250

Query: 248 SVFGVGPLSLIH-------SHSPLADSSSDEILKWLDGCPDDSVLYVSFGSQKQLNQQQM 307
            V+ +GP+SL +            A    DE L+WLD   D SVLYV  GS   L   Q+
Sbjct: 251 KVWSIGPVSLCNKAGADKAERGNQAAIDQDECLQWLDSKEDGSVLYVCLGSICNLPLSQL 310

Query: 308 EALASGLEKSGIRFLWVVK------TIHQTVGESDGIPIGFEDRVLDRGIVVRRWAPQVA 367
           + L  GLEKS   F+WV++       +++ + ES     GFE+R+ +RG++++ W+PQV 
Sbjct: 311 KELGLGLEKSQRSFIWVIRGWEKYNELYEWMMES-----GFEERIKERGLLIKGWSPQVL 370

Query: 368 ILNHRAVGGFLSHCGWNSVLESLVSGVPIFAWAMEGDQMMNSKILVD------HVGVAVQ 427
           IL+H +VGGFL+HCGWNS LE + SG+P+  W + GDQ  N K++V         GV   
Sbjct: 371 ILSHPSVGGFLTHCGWNSTLEGITSGIPLITWPLFGDQFCNQKLVVQVLKAGVSAGVEEV 430

Query: 428 VCHGENS----VPDSDQLGKVIAE----SFNSDELKAKAKALSKAVVDAAGPNGSS 448
           +  GE      + D + + K + E    S ++ E + + K L ++   A    GSS
Sbjct: 431 MKWGEEEKIGVLVDKEGVKKAVEELMGASDDAKERRRRVKELGESAHKAVEEGGSS 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZD81.1e-11849.78UDP-glycosyltransferase 89A2 OS=Arabidopsis thaliana OX=3702 GN=UGT89A2 PE=2 SV=... [more]
Q6VAA59.0e-10545.22UDP-glycosyltransferase 89B2 OS=Stevia rebaudiana OX=55670 GN=UGT89B2 PE=2 SV=1[more]
Q9C9B02.2e-10343.82Flavonol 3-O-glucosyltransferase UGT89B1 OS=Arabidopsis thaliana OX=3702 GN=UGT8... [more]
Q9LNE68.5e-7136.89Flavonol 7-O-rhamnosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT89C1 PE=... [more]
K4GKX27.2e-6231.39UDP-glycosyltransferase 73C10 OS=Barbarea vulgaris OX=50459 GN=UGT73C10 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1GYG27.4e-22780.90Glycosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111458431 PE=3 SV=1[more]
A0A6J1IRU42.6e-22480.90Glycosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111477873 PE=3 SV=1[more]
A0A6J1FZE52.2e-17064.87UDP-glycosyltransferase 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111449303 ... [more]
A0A346A6C15.4e-16965.30Flavonoids UDP-glycosyltransferase OS=Siraitia grosvenorii OX=190515 GN=UGT89M1 ... [more]
A0A6J1HY919.1e-16964.24UDP-glycosyltransferase 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111467348 PE... [more]
Match NameE-valueIdentityDescription
XP_022956873.11.5e-22680.90UDP-glycosyltransferase 89A2-like [Cucurbita moschata] >XP_023536862.1 UDP-glyco... [more]
KAG6601355.19.9e-22680.69UDP-glycosyltransferase 89A2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022977594.15.4e-22480.90UDP-glycosyltransferase 89A2-like [Cucurbita maxima][more]
XP_022944915.14.5e-17064.87UDP-glycosyltransferase 89A2-like [Cucurbita moschata][more]
XP_023542842.15.9e-17064.44UDP-glycosyltransferase 89A2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G03490.17.8e-12049.78UDP-Glycosyltransferase superfamily protein [more]
AT1G51210.11.2e-11750.12UDP-Glycosyltransferase superfamily protein [more]
AT1G73880.11.6e-10443.82UDP-glucosyl transferase 89B1 [more]
AT1G06000.16.0e-7236.89UDP-Glycosyltransferase superfamily protein [more]
AT2G36770.15.7e-6231.93UDP-Glycosyltransferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002213UDP-glucuronosyl/UDP-glucosyltransferasePFAMPF00201UDPGTcoord: 256..409
e-value: 1.7E-21
score: 76.6
IPR002213UDP-glucuronosyl/UDP-glucosyltransferaseCDDcd03784GT1_Gtf-likecoord: 10..419
e-value: 2.35757E-66
score: 216.649
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 9..264
e-value: 3.9E-51
score: 175.9
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 265..441
e-value: 4.2E-57
score: 195.0
NoneNo IPR availablePANTHERPTHR48047GLYCOSYLTRANSFERASEcoord: 9..459
NoneNo IPR availablePANTHERPTHR48047:SF28UDP-GLYCOSYLTRANSFERASE 89A2coord: 9..459
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 10..459
IPR035595UDP-glycosyltransferase family, conserved sitePROSITEPS00375UDPGTcoord: 340..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G003390.1Lsi04G003390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009536 plastid
molecular_function GO:0008194 UDP-glycosyltransferase activity