Lsi04G002750 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G002750
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
Locationchr04: 2793420 .. 2794475 (-)
RNA-Seq ExpressionLsi04G002750
SyntenyLsi04G002750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGACAAAAAGAATGCCTACCATTAACCTAGATCAAATTCCAAGTTTTGAAGAACATGGTAATGGCTTTAATCAACATGACGTGTTGGTTGAGGAGATTGATGGCCTGATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCCCAAATAGTTCCATGTGTCTCTAATGCATTGCCGCCAGAGCTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAACTGACCAACATTTGGGCTCGTTTCTATATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGATTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCAAAAGCCGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCAGCTCCTTATGAAGATGGATTAAAGACCCTTCAGTGGTTGAAACAAGTTGCTTTTGTAGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTAGTGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCCGCCGGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTAGTTGTTAAAGGTAACATTTTGATACAACCATTTTTTGGTGGAGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGTTGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCGGGACAAACCGTGACCACCCATGGTGCAATCCTTCGGCAAAAGGATTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAATTGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGCCGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAAACTCGGACCCTTGAAATGATTGATCATATCAAGGACTTCTTGTGTGCATAG

mRNA sequence

ATGAGGACAAAAAGAATGCCTACCATTAACCTAGATCAAATTCCAAGTTTTGAAGAACATGGTAATGGCTTTAATCAACATGACGTGTTGGTTGAGGAGATTGATGGCCTGATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCCCAAATAGTTCCATGTGTCTCTAATGCATTGCCGCCAGAGCTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAACTGACCAACATTTGGGCTCGTTTCTATATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGATTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCAAAAGCCGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCAGCTCCTTATGAAGATGGATTAAAGACCCTTCAGTGGTTGAAACAAGTTGCTTTTGTAGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTAGTGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCCGCCGGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTAGTTGTTAAAGGTAACATTTTGATACAACCATTTTTTGGTGGAGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGTTGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCGGGACAAACCGTGACCACCCATGGTGCAATCCTTCGGCAAAAGGATTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAATTGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGCCGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAAACTCGGACCCTTGAAATGATTGATCATATCAAGGACTTCTTGTGTGCATAG

Coding sequence (CDS)

ATGAGGACAAAAAGAATGCCTACCATTAACCTAGATCAAATTCCAAGTTTTGAAGAACATGGTAATGGCTTTAATCAACATGACGTGTTGGTTGAGGAGATTGATGGCCTGATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCCCAAATAGTTCCATGTGTCTCTAATGCATTGCCGCCAGAGCTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAACTGACCAACATTTGGGCTCGTTTCTATATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGATTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCAAAAGCCGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCAGCTCCTTATGAAGATGGATTAAAGACCCTTCAGTGGTTGAAACAAGTTGCTTTTGTAGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTAGTGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCCGCCGGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTAGTTGTTAAAGGTAACATTTTGATACAACCATTTTTTGGTGGAGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGTTGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCGGGACAAACCGTGACCACCCATGGTGCAATCCTTCGGCAAAAGGATTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAATTGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGCCGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAAACTCGGACCCTTGAAATGATTGATCATATCAAGGACTTCTTGTGTGCATAG

Protein sequence

MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCA
Homology
BLAST of Lsi04G002750 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.7e-95
Identity = 170/332 (51.20%), Postives = 228/332 (68.67%), Query Frame = 0

Query: 22  NGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTNIWAR 81
           N  N H  +V+E++GLI+VY +G+VER Q++PCV  +LP ELGVT  DVV+DKLTN+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 82  FYIPTQCQE----KLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAP 141
            Y+P    +    KLPLIVYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 142 ENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAGLG 201
           ENPLPA YEDG+  + WL +        N W++ CDF +I+L+GDSAGGNIA  VAA L 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 202 GKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALP 261
                +       L ++G ILIQPF+ GE RT+SE+ +     + LTLA SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 262 SGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLINYVV 321
            G NR+HP+C P       ++   V  +L+C++E+D+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNE---DVIKRVL 314

Query: 322 YEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lsi04G002750 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.7e-85
Identity = 162/336 (48.21%), Postives = 220/336 (65.48%), Query Frame = 0

Query: 21  GNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTN-IW 80
           G+  ++H  +VEEI+GLI+V+ +G VERP IVP VS  + P    T++D+   KL+N  W
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDI---KLSNDTW 78

Query: 81  ARFYIPTQCQEK----LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRL 140
            R YIP          LPL+VYFHGGGFCVGSAAWSCYH+FL  L+ KA C+I+SVNYRL
Sbjct: 79  TRVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRL 138

Query: 141 APENPLPAPYEDGLKTLQWL--KQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVA 200
           APE+ LPA Y+DG+  + WL  +Q++  G   +W S+ C+ + ++L+GDSAG NIA+ VA
Sbjct: 139 APEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVA 198

Query: 201 AGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWR 260
                +  ASG      L +KG ILI PFFGGESRT SEK       S LTL+ SD YWR
Sbjct: 199 V----RIMASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWR 258

Query: 261 LALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLI 320
           LALP G +RDHPWCNP    L      ++  +++ ++E DILK+RNLE C  +   GK +
Sbjct: 259 LALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRV 318

Query: 321 NYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
             +V+ GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 319 EGIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lsi04G002750 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.1e-50
Identity = 109/320 (34.06%), Postives = 174/320 (54.37%), Query Frame = 0

Query: 30  LVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSW--DVVVDKLTNIWARFYIPTQ 89
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K  N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 90  CQEK--LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 149
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 150 EDGLKTLQWLKQVAFVGAKQNWW--SRYCDFTKIYLSGDSAGGNIAFNVAAGLGGKTTAS 209
           ED    L WL   A      +W+      DF ++++ GDS+GGNIA  +A   G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 210 GAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALPSGTNRD 269
           G++ L P+ V+G +L+ PFFGGE RT SE     P  + L+L + D +WRL+LP+G  RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 270 HPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHR-AGKLINYVVYEGVG 329
           H   NP       +E + + P L+ +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 330 HAFQVLNKSQLSQTRTLEMI 343
           H F     S  +  + L +I
Sbjct: 310 HGFYSNYPSSEAAEQVLRII 321

BLAST of Lsi04G002750 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.1e-44
Identity = 106/301 (35.22%), Postives = 165/301 (54.82%), Query Frame = 0

Query: 46  VERPQIVPCVSNALPPELG--VTSWDVVVDKLTNIWARFYIPTQC---QEKLPLIVYFHG 105
           + RP  +P  + +  P     V + D+ ++ L N + R ++P        KLPL+VYFHG
Sbjct: 32  ITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHG 91

Query: 106 GGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF 165
           GGF + SAA + +H+F  +++  A  +I SV+YRLAPE+ LPA Y+D ++ LQW+K    
Sbjct: 92  GGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD--- 151

Query: 166 VGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQ 225
             ++  W + + DF+  ++ G+SAGGNIA++      G   A+ A  L PL +KG +L +
Sbjct: 152 --SRDEWLTNFADFSNCFIMGESAGGNIAYH-----AGLRAAAVADELLPLKIKGLVLDE 211

Query: 226 PFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKG--LFKVE 285
           P FGG  RT SE  L    R P    V D  W L+LP G +RDH +CNP+A+   L+  +
Sbjct: 212 PGFGGSKRTGSELRLANDSRLP--TFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFD 271

Query: 286 DLRVLPSLICI--SELDILKDRNLEFCSALHRAGKLINYVVYEGVG--HAFQVLNKSQLS 336
            +R L   + +     D + DR +E    L + G  ++ V    VG  HA ++ +  +  
Sbjct: 272 KIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKG--VDVVAQFDVGGYHAVKLEDPEKAK 318

BLAST of Lsi04G002750 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 5.3e-41
Identity = 108/336 (32.14%), Postives = 168/336 (50.00%), Query Frame = 0

Query: 30  LVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTNIWARFYIPTQC- 89
           L+   DG    +   +++R   VP  +N   P  GV S+DV++D+ TN+ +R Y P    
Sbjct: 33  LLRRPDGTFNRHLAEFLDRK--VPANAN---PVNGVFSFDVIIDRQTNLLSRVYRPADAG 92

Query: 90  -------------QEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRL 149
                         E +P+IV+FHGG F   SA  + Y     +L      +++SVNYR 
Sbjct: 93  TSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRR 152

Query: 150 APENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAG 209
           APEN  P  Y+DG   L+W+   +++ +K++         +I+L+GDS+GGNI  NVA  
Sbjct: 153 APENRYPCAYDDGWAVLKWVNSSSWLRSKKD------SKVRIFLAGDSSGGNIVHNVAV- 212

Query: 210 LGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLA 269
                      V   + V GNIL+ P FGG  RT+SEK L    +  +T+   D YWR  
Sbjct: 213 ---------RAVESRIDVLGNILLNPMFGGTERTESEKRL--DGKYFVTVRDRDWYWRAF 272

Query: 270 LPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLINY 329
           LP G +R+HP C+P       +E L    SL+ ++ LD+++D  L++   L +AG+ +  
Sbjct: 273 LPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKL 332

Query: 330 VVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCA 352
           +  E     F +L  +    T    ++D I  F+ A
Sbjct: 333 LYLEQATIGFYLLPNNNHFHT----VMDEIAAFVNA 341

BLAST of Lsi04G002750 vs. ExPASy TrEMBL
Match: A0A0A0KUG5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608500 PE=4 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.0e-200
Identity = 335/350 (95.71%), Postives = 342/350 (97.71%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLS
Sbjct: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLTL VSDTYWRLALPSGTNRDHPWCNPS KGLF VEDLRVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIK FLC
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLC 350

BLAST of Lsi04G002750 vs. ExPASy TrEMBL
Match: A0A5A7SV76 (Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00820 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 4.9e-199
Identity = 331/350 (94.57%), Postives = 340/350 (97.14%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTK+MPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKKMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAK+DCII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLS
Sbjct: 121 SAKSDCIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRT SEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPR 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLTL VSDTYWRLALPSGTNRDHPWCNPS KGLF VED RVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFLC
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLC 350

BLAST of Lsi04G002750 vs. ExPASy TrEMBL
Match: A0A1S3BFC8 (probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.3e-196
Identity = 327/345 (94.78%), Postives = 335/345 (97.10%), Query Frame = 0

Query: 6   MPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGV 65
           MPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALPPELGV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 66  TSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 125
           TSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAK+D
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAG 185
           CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLSGDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 186 GNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTL 245
           GNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRT SEKFLVQPPRSPLTL
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 246 AVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSA 305
            VSDTYWRLALPSGTNRDHPWCNPS KGLF VED RVLPSLICISE+DILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFLC
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLC 345

BLAST of Lsi04G002750 vs. ExPASy TrEMBL
Match: A0A6J1J448 (probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111481140 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 324/349 (92.84%), Postives = 335/349 (95.99%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEHGNGF+QH VLVEEI+GLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA+WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF GAKQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAVV+KPLV+KGNILIQPFFGGESRTKSEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPH 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLT AVSDTYWRLALPS  NRDHPWCNPSA G+F+VEDLRVLPSLICISE+DILKDR+L
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIKDFL
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348

BLAST of Lsi04G002750 vs. ExPASy TrEMBL
Match: A0A6J1H254 (probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111458883 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.6e-192
Identity = 324/349 (92.84%), Postives = 333/349 (95.42%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEHGNGF+QH VLVEEI+GLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF GAKQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAVV+KPLV+KGNILIQPFFGGESRT SEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPH 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLT AVSDTYWRLALPS  NRDHPWCNPSA G+F+VEDLRVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIKDFL
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348

BLAST of Lsi04G002750 vs. NCBI nr
Match: XP_038893358.1 (probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893360.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893361.1 probable carboxylesterase 6 [Benincasa hispida])

HSP 1 Score: 722.6 bits (1864), Expect = 1.6e-204
Identity = 342/350 (97.71%), Postives = 348/350 (99.43%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEHGNGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVV+DKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVMDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAVVLKPLV+KG+ILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVLKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 350

BLAST of Lsi04G002750 vs. NCBI nr
Match: XP_004135407.1 (probable carboxylesterase 6 [Cucumis sativus] >XP_011655765.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741302.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741303.1 probable carboxylesterase 6 [Cucumis sativus] >KGN52062.1 hypothetical protein Csa_008345 [Cucumis sativus])

HSP 1 Score: 708.0 bits (1826), Expect = 4.1e-200
Identity = 335/350 (95.71%), Postives = 342/350 (97.71%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLS
Sbjct: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLTL VSDTYWRLALPSGTNRDHPWCNPS KGLF VEDLRVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIK FLC
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLC 350

BLAST of Lsi04G002750 vs. NCBI nr
Match: KAA0034538.1 (putative carboxylesterase 6 [Cucumis melo var. makuwa] >TYK09092.1 putative carboxylesterase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 703.4 bits (1814), Expect = 1.0e-198
Identity = 331/350 (94.57%), Postives = 340/350 (97.14%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTK+MPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKKMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAK+DCII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLS
Sbjct: 121 SAKSDCIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRT SEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPR 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLTL VSDTYWRLALPSGTNRDHPWCNPS KGLF VED RVLPSLICISE+DILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFLC
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLC 350

BLAST of Lsi04G002750 vs. NCBI nr
Match: XP_008446570.1 (PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446572.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446573.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446575.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo])

HSP 1 Score: 694.5 bits (1791), Expect = 4.7e-196
Identity = 327/345 (94.78%), Postives = 335/345 (97.10%), Query Frame = 0

Query: 6   MPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGV 65
           MPTINLDQIPSFEEH NGF+QHDVLVEEI+GLIRVYGNGYVERPQIVPCVSNALPPELGV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 66  TSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 125
           TSWDVVVDKL NIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAK+D
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAG 185
           CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLSGDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 186 GNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTL 245
           GNIAFNVAA LGGKTTASGAV+LKPLV+KG+ILIQPFFGGESRT SEKFLVQPPRSPLTL
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 246 AVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSA 305
            VSDTYWRLALPSGTNRDHPWCNPS KGLF VED RVLPSLICISE+DILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLC 351
           LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFLC
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLC 345

BLAST of Lsi04G002750 vs. NCBI nr
Match: XP_022982294.1 (probable carboxylesterase 6 [Cucurbita maxima] >XP_022982375.1 probable carboxylesterase 6 [Cucurbita maxima] >XP_022982445.1 probable carboxylesterase 6 [Cucurbita maxima])

HSP 1 Score: 681.8 bits (1758), Expect = 3.1e-192
Identity = 324/349 (92.84%), Postives = 335/349 (95.99%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHGNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEHGNGF+QH VLVEEI+GLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLTNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKL NIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA+WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180
           SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF GAKQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAAGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAA LGGKTTASGAVV+KPLV+KGNILIQPFFGGESRTKSEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPH 240

Query: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNL 300
           SPLT AVSDTYWRLALPS  NRDHPWCNPSA G+F+VEDLRVLPSLICISE+DILKDR+L
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIKDFL
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348

BLAST of Lsi04G002750 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.6e-96
Identity = 170/332 (51.20%), Postives = 228/332 (68.67%), Query Frame = 0

Query: 22  NGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTNIWAR 81
           N  N H  +V+E++GLI+VY +G+VER Q++PCV  +LP ELGVT  DVV+DKLTN+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 82  FYIPTQCQE----KLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAP 141
            Y+P    +    KLPLIVYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 142 ENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAGLG 201
           ENPLPA YEDG+  + WL +        N W++ CDF +I+L+GDSAGGNIA  VAA L 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 202 GKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALP 261
                +       L ++G ILIQPF+ GE RT+SE+ +     + LTLA SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 262 SGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLINYVV 321
            G NR+HP+C P       ++   V  +L+C++E+D+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNE---DVIKRVL 314

Query: 322 YEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lsi04G002750 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 317.0 bits (811), Expect = 1.9e-86
Identity = 162/336 (48.21%), Postives = 220/336 (65.48%), Query Frame = 0

Query: 21  GNGFNQHDVLVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTN-IW 80
           G+  ++H  +VEEI+GLI+V+ +G VERP IVP VS  + P    T++D+   KL+N  W
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDI---KLSNDTW 78

Query: 81  ARFYIPTQCQEK----LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRL 140
            R YIP          LPL+VYFHGGGFCVGSAAWSCYH+FL  L+ KA C+I+SVNYRL
Sbjct: 79  TRVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRL 138

Query: 141 APENPLPAPYEDGLKTLQWL--KQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVA 200
           APE+ LPA Y+DG+  + WL  +Q++  G   +W S+ C+ + ++L+GDSAG NIA+ VA
Sbjct: 139 APEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVA 198

Query: 201 AGLGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWR 260
                +  ASG      L +KG ILI PFFGGESRT SEK       S LTL+ SD YWR
Sbjct: 199 V----RIMASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWR 258

Query: 261 LALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLI 320
           LALP G +RDHPWCNP    L      ++  +++ ++E DILK+RNLE C  +   GK +
Sbjct: 259 LALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRV 318

Query: 321 NYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 350
             +V+ GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 319 EGIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lsi04G002750 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-51
Identity = 109/320 (34.06%), Postives = 174/320 (54.37%), Query Frame = 0

Query: 30  LVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSW--DVVVDKLTNIWARFYIPTQ 89
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K  N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 90  CQEK--LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 149
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 150 EDGLKTLQWLKQVAFVGAKQNWW--SRYCDFTKIYLSGDSAGGNIAFNVAAGLGGKTTAS 209
           ED    L WL   A      +W+      DF ++++ GDS+GGNIA  +A   G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 210 GAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALPSGTNRD 269
           G++ L P+ V+G +L+ PFFGGE RT SE     P  + L+L + D +WRL+LP+G  RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 270 HPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHR-AGKLINYVVYEGVG 329
           H   NP       +E + + P L+ +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 330 HAFQVLNKSQLSQTRTLEMI 343
           H F     S  +  + L +I
Sbjct: 310 HGFYSNYPSSEAAEQVLRII 321

BLAST of Lsi04G002750 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-51
Identity = 109/320 (34.06%), Postives = 174/320 (54.37%), Query Frame = 0

Query: 30  LVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSW--DVVVDKLTNIWARFYIPTQ 89
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K  N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 90  CQEK--LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 149
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 150 EDGLKTLQWLKQVAFVGAKQNWW--SRYCDFTKIYLSGDSAGGNIAFNVAAGLGGKTTAS 209
           ED    L WL   A      +W+      DF ++++ GDS+GGNIA  +A   G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 210 GAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLALPSGTNRD 269
           G++ L P+ V+G +L+ PFFGGE RT SE     P  + L+L + D +WRL+LP+G  RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 270 HPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHR-AGKLINYVVYEGVG 329
           H   NP       +E + + P L+ +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 330 HAFQVLNKSQLSQTRTLEMI 343
           H F     S  +  + L +I
Sbjct: 310 HGFYSNYPSSEAAEQVLRII 321

BLAST of Lsi04G002750 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 3.7e-42
Identity = 108/336 (32.14%), Postives = 168/336 (50.00%), Query Frame = 0

Query: 30  LVEEIDGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLTNIWARFYIPTQC- 89
           L+   DG    +   +++R   VP  +N   P  GV S+DV++D+ TN+ +R Y P    
Sbjct: 33  LLRRPDGTFNRHLAEFLDRK--VPANAN---PVNGVFSFDVIIDRQTNLLSRVYRPADAG 92

Query: 90  -------------QEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRL 149
                         E +P+IV+FHGG F   SA  + Y     +L      +++SVNYR 
Sbjct: 93  TSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRR 152

Query: 150 APENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAAG 209
           APEN  P  Y+DG   L+W+   +++ +K++         +I+L+GDS+GGNI  NVA  
Sbjct: 153 APENRYPCAYDDGWAVLKWVNSSSWLRSKKD------SKVRIFLAGDSSGGNIVHNVAV- 212

Query: 210 LGGKTTASGAVVLKPLVVKGNILIQPFFGGESRTKSEKFLVQPPRSPLTLAVSDTYWRLA 269
                      V   + V GNIL+ P FGG  RT+SEK L    +  +T+   D YWR  
Sbjct: 213 ---------RAVESRIDVLGNILLNPMFGGTERTESEKRL--DGKYFVTVRDRDWYWRAF 272

Query: 270 LPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISELDILKDRNLEFCSALHRAGKLINY 329
           LP G +R+HP C+P       +E L    SL+ ++ LD+++D  L++   L +AG+ +  
Sbjct: 273 LPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKL 332

Query: 330 VVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCA 352
           +  E     F +L  +    T    ++D I  F+ A
Sbjct: 333 LYLEQATIGFYLLPNNNHFHT----VMDEIAAFVNA 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX253.7e-9551.20Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR72.7e-8548.21Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9FG132.1e-5034.06Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV75.1e-4435.22Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q940G65.3e-4132.14Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KUG52.0e-20095.71Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6085... [more]
A0A5A7SV764.9e-19994.57Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BFC82.3e-19694.78probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1[more]
A0A6J1J4481.5e-19292.84probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111481140 PE=4 SV=... [more]
A0A6J1H2542.6e-19292.84probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111458883 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_038893358.11.6e-20497.71probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxy... [more]
XP_004135407.14.1e-20095.71probable carboxylesterase 6 [Cucumis sativus] >XP_011655765.1 probable carboxyle... [more]
KAA0034538.11.0e-19894.57putative carboxylesterase 6 [Cucumis melo var. makuwa] >TYK09092.1 putative carb... [more]
XP_008446570.14.7e-19694.78PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446572.1 PREDICTED:... [more]
XP_022982294.13.1e-19292.84probable carboxylesterase 6 [Cucurbita maxima] >XP_022982375.1 probable carboxyl... [more]
Match NameE-valueIdentityDescription
AT1G68620.12.6e-9651.20alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.11.9e-8648.21carboxyesterase 17 [more]
AT5G06570.11.5e-5134.06alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.5e-5134.06alpha/beta-Hydrolases superfamily protein [more]
AT5G27320.13.7e-4232.14alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 95..326
e-value: 1.0E-53
score: 182.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 49..351
e-value: 8.0E-72
score: 244.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 30..349
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 20..349
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 20..349
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 177..189

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G002750.1Lsi04G002750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity