Lsi04G002460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G002460
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	adenylate isopentenyltransferase-like
Location	chr04: 2510424 .. 2511446 (-)
RNA-Seq Expression	Lsi04G002460
Synteny	Lsi04G002460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTGTTGATTCAACGTCTTGCTCTAATCTTATGACACTCCCTTCTTCTTCTTCTTCTTATTCTTCAGCTAAAATCTCCTTTCTAAAGCTTCCCTTTTCTCGCCGGAGAAAATGGCAGCGAGTGAAGGCCATTGCCGGCGCCGCCGGGAAAGAGATCAAGATTCTCGTGATTATGGGCGCCACCGGGTGCGGCAAATCCGGCTTATCCGTTCAACTAGCCTCTCATTTTCCATCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAAAGGTCTTGACATTACGACTAACAAGATTCCCTTTCACGAACGACATGACGTCCCACACCATTTACTCGGCGACGTCGATTCCCTTCACGAAGAATTCACTCCCTTTCATTACAGAGTCCGTGCCGACAATGTCGTTTCAGATATAGCCTGTAGGAAGAAACTACCTATTCTCGTAGGTGGGTCCAATTCCTTCATTCACGCATTGCTCGTGAACCATTTCAATCCAAACGACGACGTTTTCTCTCATATTCCCAAATCCGTTATTTCATCCGATTTACGTTACCGTTGTTGCTTTCTCTGGTTAGACGTCGCGTTTCCCATTTTGTCAGAATATCTTTCAATCCGAGTTGATGAAATGCTCCAACTCGGAATGTTTGAGGAATTAGCCGAGTTTTATGATCCCGAAACGGCGGAAACGACGCCGTATACTGGGATTAGAAAAGCAATAGGAGTGCCGGAGTTTGATAGGTACTTCAAAAAAAACCCGCCGAGTAAAAAAAACGGGATTGATAGAGAGGCGTTTGAAGAAGCGGTTAATGCCATAAAAAGAAACACGCACGTGCTGGCGGAGAGACAGATAAGGAAAATTTTGAAACTGAAAGGGGCGGGCTGGGATATGCACGTGCTCAAGGCGACGGAGGCGTTTAGGGCGGTGGTGGAACCGGGATCCCGCCGGAACCGGAAGGAGATTTGGGAGAAGCAGATTTTGGAACCGAGTTTGAGGATTGTAAAGCGCTTCTTGGAGGAGTAG

mRNA sequence

ATGCGTGTTGATTCAACGTCTTGCTCTAATCTTATGACACTCCCTTCTTCTTCTTCTTCTTATTCTTCAGCTAAAATCTCCTTTCTAAAGCTTCCCTTTTCTCGCCGGAGAAAATGGCAGCGAGTGAAGGCCATTGCCGGCGCCGCCGGGAAAGAGATCAAGATTCTCGTGATTATGGGCGCCACCGGGTGCGGCAAATCCGGCTTATCCGTTCAACTAGCCTCTCATTTTCCATCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAAAGGTCTTGACATTACGACTAACAAGATTCCCTTTCACGAACGACATGACGTCCCACACCATTTACTCGGCGACGTCGATTCCCTTCACGAAGAATTCACTCCCTTTCATTACAGAGTCCGTGCCGACAATGTCGTTTCAGATATAGCCTGTAGGAAGAAACTACCTATTCTCGTAGGTGGGTCCAATTCCTTCATTCACGCATTGCTCGTGAACCATTTCAATCCAAACGACGACGTTTTCTCTCATATTCCCAAATCCGTTATTTCATCCGATTTACGTTACCGTTGTTGCTTTCTCTGGTTAGACGTCGCGTTTCCCATTTTGTCAGAATATCTTTCAATCCGAGTTGATGAAATGCTCCAACTCGGAATGTTTGAGGAATTAGCCGAGTTTTATGATCCCGAAACGGCGGAAACGACGCCGTATACTGGGATTAGAAAAGCAATAGGAGTGCCGGAGTTTGATAGGTACTTCAAAAAAAACCCGCCGAGTAAAAAAAACGGGATTGATAGAGAGGCGTTTGAAGAAGCGGTTAATGCCATAAAAAGAAACACGCACGTGCTGGCGGAGAGACAGATAAGGAAAATTTTGAAACTGAAAGGGGCGGGCTGGGATATGCACGTGCTCAAGGCGACGGAGGCGTTTAGGGCGGTGGTGGAACCGGGATCCCGCCGGAACCGGAAGGAGATTTGGGAGAAGCAGATTTTGGAACCGAGTTTGAGGATTGTAAAGCGCTTCTTGGAGGAGTAG

Coding sequence (CDS)

ATGCGTGTTGATTCAACGTCTTGCTCTAATCTTATGACACTCCCTTCTTCTTCTTCTTCTTATTCTTCAGCTAAAATCTCCTTTCTAAAGCTTCCCTTTTCTCGCCGGAGAAAATGGCAGCGAGTGAAGGCCATTGCCGGCGCCGCCGGGAAAGAGATCAAGATTCTCGTGATTATGGGCGCCACCGGGTGCGGCAAATCCGGCTTATCCGTTCAACTAGCCTCTCATTTTCCATCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAAAGGTCTTGACATTACGACTAACAAGATTCCCTTTCACGAACGACATGACGTCCCACACCATTTACTCGGCGACGTCGATTCCCTTCACGAAGAATTCACTCCCTTTCATTACAGAGTCCGTGCCGACAATGTCGTTTCAGATATAGCCTGTAGGAAGAAACTACCTATTCTCGTAGGTGGGTCCAATTCCTTCATTCACGCATTGCTCGTGAACCATTTCAATCCAAACGACGACGTTTTCTCTCATATTCCCAAATCCGTTATTTCATCCGATTTACGTTACCGTTGTTGCTTTCTCTGGTTAGACGTCGCGTTTCCCATTTTGTCAGAATATCTTTCAATCCGAGTTGATGAAATGCTCCAACTCGGAATGTTTGAGGAATTAGCCGAGTTTTATGATCCCGAAACGGCGGAAACGACGCCGTATACTGGGATTAGAAAAGCAATAGGAGTGCCGGAGTTTGATAGGTACTTCAAAAAAAACCCGCCGAGTAAAAAAAACGGGATTGATAGAGAGGCGTTTGAAGAAGCGGTTAATGCCATAAAAAGAAACACGCACGTGCTGGCGGAGAGACAGATAAGGAAAATTTTGAAACTGAAAGGGGCGGGCTGGGATATGCACGTGCTCAAGGCGACGGAGGCGTTTAGGGCGGTGGTGGAACCGGGATCCCGCCGGAACCGGAAGGAGATTTGGGAGAAGCAGATTTTGGAACCGAGTTTGAGGATTGTAAAGCGCTTCTTGGAGGAGTAG

Protein sequence

MRVDSTSCSNLMTLPSSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAAGKEIKILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE

Homology

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BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.5e-93
Identity = 181/299 (60.54%), Postives = 219/299 (73.24%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K+LV+MGATG GKS LS+ LA+HFP E+INSDKMQVYKGLDITTNKI   +R  VPHHLL
Sbjct: 31  KLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHLL 90

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPN-DDVFS 173
           G+VD    E TP  +R  A   VS+I  R+KLP+LVGGSNSFIHALLV+ F+ +   VF 
Sbjct: 91  GEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVFE 150

Query: 174 HIPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPE----TA 233
               SV+SS+LRY CCFLW+DV+  +L++YL+ RVD+ML+LGMF+ELAEFY PE      
Sbjct: 151 EGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHDE 210

Query: 234 ETTPYTGIRKAIGVPEFDRYFKKNPPSKKNGID-------REAFEEAVNAIKRNTHVLAE 293
           ++   TG+RKAIGVPEFDRYF+K  P    G D       R AFEEAV AIK NT  LA+
Sbjct: 211 DSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHLAK 270

Query: 294 RQIRKILKLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           RQI KIL+LKGAGWD+  L ATE+FRA +   S     EIWEKQ+LEPS++IV RFL+E
Sbjct: 271 RQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFLDE 329

[Close]

BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 172/335 (51.34%), Postives = 230/335 (68.66%), Query Frame = 0


Query: 13  TLPSSSSSYSSAKISFLKLPFSRRRKWQRVKAI----AGAAGKEIKILVIMGATGCGKSG 72
           T   S SS+SS+  S L     RR+    V +I    + +  ++ K++VI+GATG GKS 
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSR 80

Query: 73  LSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHY 132
           LSV LA+ FPSEIINSDK+QVY+GL+ITTN+I   +R  VPHHLLG ++  H E T   +
Sbjct: 81  LSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEF 140

Query: 133 RVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLRYRCC 192
           R  A NVV +I  R+K+PI+ GGSNSF+HALL   F+P  D FS     +ISSDLRY CC
Sbjct: 141 RSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSS-GSCLISSDLRYECC 200

Query: 193 FLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRY 252
           F+W+DV+  +L EYL  RVDEM+  GMFEEL+ FYDP  +      GIRKAIGVPEFD Y
Sbjct: 201 FIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGY 260

Query: 253 FKKNPPSKK----NGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 312
           FK+ PP KK    + + + A+++AV+ IKRNT  LA+RQ++KI  LK AGW++  + AT 
Sbjct: 261 FKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATA 320

Query: 313 AFRAV-VEPGSRRNRKEIWEKQILEPSLRIVKRFL 339
           +F+AV ++  S +  +E WE+Q+LEPS++IVKR L
Sbjct: 321 SFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.6e-73
Identity = 146/292 (50.00%), Postives = 193/292 (66.10%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K++VIMGATG GKS LS+ LA+ F  EI+NSDK+Q Y GL +TTN++   ER  VPHHLL
Sbjct: 44  KVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLL 103

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH 173
           G++     E T   +R  A   +S+I  R  LPI+ GGSNSFIHALLV+ F+P    FS 
Sbjct: 104 GELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFS- 163

Query: 174 IPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPE-TAETTP 233
             ++ ISS LRY CCFLW+DV+  +L EYLS RVD+M++ GMFEELA FYDP  +     
Sbjct: 164 -SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 223

Query: 234 YTGIRKAIGVPEFDRYFKKNPPSKKNGID------REAFEEAVNAIKRNTHVLAERQIRK 293
             GI K IG+PEFDRYF   PP +K  +       + A++EAV  IK NT  LA++QI +
Sbjct: 224 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 283

Query: 294 ILKLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFL 339
           I+KLK +GWD+  L AT +F         R+ +EIW+  +L+ S+++VKRFL
Sbjct: 284 IMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

[Close]

BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 147/296 (49.66%), Postives = 195/296 (65.88%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K++VIMGATG GKS LSV LA HF +EIINSDKMQ Y GL ITTN+    +R  VPHHLL
Sbjct: 6   KMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLL 65

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH 173
           G+++    E T   +RV A   +S+I  RKKLPIL GGSNS+IHALL   ++P +  FS 
Sbjct: 66  GELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSD 125

Query: 174 IPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPY 233
             K  I S+L+Y CCF+W+DV   +L EYLS+R+D M++ GMFEE+AEF+  + A   P 
Sbjct: 126 -HKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP- 185

Query: 234 TGIRKAIGVPEFDRYFK----KNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILK 293
            GI KAIGV EFD Y K     N   K + + +EA+E+AV AIK NT  L + QI KI K
Sbjct: 186 LGIWKAIGVQEFDDYLKMYKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 245

Query: 294 LKGAGWDMHVLKATEAFRAVVE-----PGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           L+ AGWD+  + AT +FR  +       G    +++IW K++LEP ++IV+  L++
Sbjct: 246 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 9.9e-69
Identity = 157/340 (46.18%), Postives = 204/340 (60.00%), Query Frame = 0


Query: 11  LMTLPSSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAAGKEIKILVIMGATGCGKSGLS 70
           LMTL S   S+SS  +  +   F   R        AG    + K+++I G TG GKS LS
Sbjct: 4   LMTLLSPPLSHSSL-LPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLS 63

Query: 71  VQLASH-FPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYR 130
           V LA+  FP+EIINSDKMQ+YKG +I TN IP HE+  VPHHLLG       E TP  +R
Sbjct: 64  VDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFR 123

Query: 131 VRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFS-HIPKSVISSDLRYRCC 190
             A   +S +   KKLPI+VGGSNSF HALL   F+P+ D FS     S I SDLRY+CC
Sbjct: 124 SLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCC 183

Query: 191 FLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRY 250
            LW+DV  P+L ++L  RVD+M++ G+ E+LAE YDP   ++    G+RK IGV EFDRY
Sbjct: 184 ILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRY 243

Query: 251 FKKNPPSKKNGI----DREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 310
           F+  P     GI     + A+EE V  +K  T  L ++Q  KI+KL   GW++  L AT 
Sbjct: 244 FRVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATA 303

Query: 311 AFRAVVE----PGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           A  A +      G  +N +EIWEK I++ S+ IVK+FL E
Sbjct: 304 AIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL
Match: A0A0A0KR70 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609740 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.1e-152
Identity = 284/352 (80.68%), Postives = 308/352 (87.50%), Query Frame = 0


Query: 1   MRVDSTSCSNLMTL----------PSSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAA- 60
           MRV S S  NLMTL           SSSSS SS+KI  L +PFSRR+K Q+VKAIA AA 
Sbjct: 1   MRVGSASSFNLMTLSSSSYSSSSSSSSSSSSSSSKIFILNIPFSRRKKRQQVKAIAAAAT 60

Query: 61  -GKEIKILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDV 120
            GK+  ILVIMGATGCGKSGLSVQLASH+ SEIIN DKMQVYKGLDITTNKIP HERHDV
Sbjct: 61  TGKDQNILVIMGATGCGKSGLSVQLASHYQSEIINCDKMQVYKGLDITTNKIPLHERHDV 120

Query: 121 PHHLLGDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPND 180
           PHHLLGDVDS+H++FTPFH+R+RADNVVSDIA R KLPILVGGSNSFIHA+LVNHFNPN 
Sbjct: 121 PHHLLGDVDSIHQDFTPFHFRLRADNVVSDIASRNKLPILVGGSNSFIHAMLVNHFNPNH 180

Query: 181 DVFSHIPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETA 240
           DVF+  PKS+ISS LRYRCCFLWLDV FPIL+EYLSIRVDEMLQ+GMFEELAEFYDP TA
Sbjct: 181 DVFT--PKSLISSHLRYRCCFLWLDVEFPILAEYLSIRVDEMLQIGMFEELAEFYDPHTA 240

Query: 241 ETTPYTGIRKAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKIL 300
           ET PYTGIRKAIGVPEFD+YF+KNPPSKK+GIDREAFEEAV AIKRNTHVLAERQI KIL
Sbjct: 241 ETMPYTGIRKAIGVPEFDKYFQKNPPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKIL 300

Query: 301 KLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           KLK AGWD++VL ATEAFRAVVEPG+ RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 301 KLKEAGWDINVLNATEAFRAVVEPGTGRNRKEIWEKEILEPSFRIVNRFLKE 350

[Close]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL
Match: A0A1S4DW42 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990777 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.4e-152
Identity = 280/328 (85.37%), Postives = 298/328 (90.85%), Query Frame = 0


Query: 16  SSSSSYSSAKISFLKLPFSRRRKWQRVKAI--AGAAGKEIKILVIMGATGCGKSGLSVQL 75
           SSSSSYSS KI  L +PFSRRRK  +VKAI  A AAGKE KILVIMGATGCGKSGLSVQL
Sbjct: 5   SSSSSYSSTKIFILNIPFSRRRKRHQVKAIAAAAAAGKE-KILVIMGATGCGKSGLSVQL 64

Query: 76  ASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYRVRAD 135
           ASH+ +EIIN DKMQVYKGLDITTNKIP HERHDVPHHLLGDVDS+HE+FTPFH+R RAD
Sbjct: 65  ASHYQAEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGDVDSIHEDFTPFHFRPRAD 124

Query: 136 NVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH-IPKSVISSDLRYRCCFLWL 195
           NVVS+IA R KLPILVGGSNSFIHA+LVN FNPN DVF++ IPK +ISSDLRYRCCFLWL
Sbjct: 125 NVVSNIASRNKLPILVGGSNSFIHAMLVNRFNPNHDVFTNCIPKPLISSDLRYRCCFLWL 184

Query: 196 DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRYFKKN 255
           DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDP TAET PYTGIRKAIGVPEFD+YFKKN
Sbjct: 185 DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPHTAETMPYTGIRKAIGVPEFDKYFKKN 244

Query: 256 PPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATEAFRAVVEP 315
           PPSKK+GIDREAFEEAV AIKRNTHVLAERQI KILKLK AGWD++VL ATEAFRAVVEP
Sbjct: 245 PPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKILKLKEAGWDINVLNATEAFRAVVEP 304

Query: 316 GSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           G  RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 305 GIGRNRKEIWEKEILEPSFRIVNRFLKE 331

[Close]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL
Match: A0A5A7STW3 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001060 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.6e-138
Identity = 247/283 (87.28%), Postives = 263/283 (92.93%), Query Frame = 0


Query: 59  MGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDS 118
           MGATGCGKSGLSVQLASH+ +EIIN DKMQVYKGLDITTNKIP HERHDVPHHLLGDVDS
Sbjct: 1   MGATGCGKSGLSVQLASHYQAEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGDVDS 60

Query: 119 LHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH-IPKS 178
           +HE+FTPFH+R RADNVVS+IA R KLPILVGGSNSFIHA+LVN FNPN DVF++ IPK 
Sbjct: 61  IHEDFTPFHFRPRADNVVSNIASRNKLPILVGGSNSFIHAMLVNRFNPNHDVFTNCIPKP 120

Query: 179 VISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIR 238
           +ISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDP TAET PYTGIR
Sbjct: 121 LISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPHTAETMPYTGIR 180

Query: 239 KAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDM 298
           KAIGVPEFD+YFKKNPPSKK+GIDREAFEEAV AIKRNTHVLAERQI KILKLK AGWD+
Sbjct: 181 KAIGVPEFDKYFKKNPPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKILKLKEAGWDI 240

Query: 299 HVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           +VL ATEAFRAVVEPG  RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 241 NVLNATEAFRAVVEPGIGRNRKEIWEKEILEPSFRIVNRFLKE 283

[Close]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL
Match: A0A6J1HWS1 (adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111467363 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.4e-136
Identity = 258/336 (76.79%), Postives = 284/336 (84.52%), Query Frame = 0


Query: 12  MTLPSSSSSYS--SAKISFLKLPFSRRRKWQR-VKAIAGAA----GKEIKILVIMGATGC 71
           MTLPSSSSSY   S++ISFLK P   RR+ +R V AIA AA    GKE KILVI+GATGC
Sbjct: 1   MTLPSSSSSYCSFSSRISFLKAPLIPRRRERRFVNAIAAAATTIGGKE-KILVILGATGC 60

Query: 72  GKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFT 131
           GKSGLSV+L +HFP+EIINSDKMQVY GLDITTNKIP HERHDVPHH+LGDVDS   EFT
Sbjct: 61  GKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDVDSDKGEFT 120

Query: 132 PFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLR 191
           P  +R+RADNVVSDIA R KLPILVGGSNSFIHA+LV HFNPNDDVF+     +ISSDLR
Sbjct: 121 PLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSDLR 180

Query: 192 YRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPE 251
           YRCCFLWLDVAFPIL+EYLSIRVDEMLQLGMFEELAEFY+P TAET  Y+GIRKAIGVPE
Sbjct: 181 YRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYNPTTAETASYSGIRKAIGVPE 240

Query: 252 FDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 311
           FD+YFKK PP++ N I + AF +AVN IK NTHVLAERQI KILKLK AGWD++VL AT 
Sbjct: 241 FDKYFKKYPPARNNKIHKSAFNDAVNEIKLNTHVLAERQIGKILKLKQAGWDINVLNATA 300

Query: 312 AFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           AFRA VEPGSR NR+EIW+ QI EPSLRIV RFLEE
Sbjct: 301 AFRAAVEPGSRMNREEIWKNQIWEPSLRIVNRFLEE 335

[Close]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL
Match: A0A6J1G1X1 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449909 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.7e-132
Identity = 246/323 (76.16%), Postives = 275/323 (85.14%), Query Frame = 0


Query: 23  SAKISFLKLPFSRRRKWQR-VKAIAGAA----GKEIKILVIMGATGCGKSGLSVQLASHF 82
           S++ISFLK P   RR+ +R V AIA AA    GKE KILVI+GATGCGKSGLSV+L +HF
Sbjct: 37  SSRISFLKSPLIPRRRERRFVNAIAAAATTTGGKE-KILVILGATGCGKSGLSVRLGTHF 96

Query: 83  PSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYRVRADNVVS 142
           P+EIINSDKMQVY GLDITTNKIP HERHDVPHH+LGD+DS   EFTP  +R+RADNVVS
Sbjct: 97  PAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDIDSDKGEFTPLDFRIRADNVVS 156

Query: 143 DIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLRYRCCFLWLDVAFP 202
           DIA R KLPILVGGSNSFIHA+LV HFNPNDDVF+     +ISSDLRYRCCFLWLDVAFP
Sbjct: 157 DIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSDLRYRCCFLWLDVAFP 216

Query: 203 ILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRYFKKNPPSKK 262
           IL+EYLSIRVDEMLQLGMFEELAEFYDP TAET  Y+GIRKAIGVPEFD+YFKK PP++ 
Sbjct: 217 ILAEYLSIRVDEMLQLGMFEELAEFYDPTTAETASYSGIRKAIGVPEFDKYFKKYPPARN 276

Query: 263 NGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATEAFRAVVEPGSRRN 322
           N I + AF++AVN IK NTHVLA+RQI KILKLK AGWD++VL AT AFRA VEPG+R N
Sbjct: 277 NKIHKSAFDDAVNEIKLNTHVLAKRQIGKILKLKQAGWDINVLNATAAFRAAVEPGNRMN 336

Query: 323 RKEIWEKQILEPSLRIVKRFLEE 341
           R+EIW+ QI EPSLRIV RFLEE
Sbjct: 337 REEIWKNQIWEPSLRIVNRFLEE 358

[Close]

BLAST of Lsi04G002460 vs. NCBI nr
Match: XP_038893291.1 (adenylate isopentenyltransferase [Benincasa hispida])

HSP 1 Score: 575.9 bits (1483), Expect = 2.4e-160
Identity = 295/347 (85.01%), Postives = 314/347 (90.49%), Query Frame = 0


Query: 1   MRVDSTSCSNLMTLP-----SSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAAGKEIKI 60
           MR+DSTS  NLMTLP     SSS+S SS   + L LPFSRRRKWQRVKAIAG  G++ KI
Sbjct: 1   MRIDSTSSFNLMTLPPPPSSSSSTSSSSYSSALLNLPFSRRRKWQRVKAIAG--GEKEKI 60

Query: 61  LVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGD 120
           LVIMGATGCGKSGLSVQLASHF SEIIN DKMQVYKGLDITTNKIP HERHDVPHHLLGD
Sbjct: 61  LVIMGATGCGKSGLSVQLASHFQSEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGD 120

Query: 121 VDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVF---S 180
           VDSLHEEFTPFH+R+RADNVVSDI  R KLPILVGGSNSFIHA+LVNHFNP DDVF   S
Sbjct: 121 VDSLHEEFTPFHFRLRADNVVSDICSRNKLPILVGGSNSFIHAMLVNHFNPMDDVFLTHS 180

Query: 181 HIPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTP 240
           HIPKS+ISSDLRYRCCFLWLDV+FPILSEYLSIRVDEMLQLGMFEELAEFY P+TAETTP
Sbjct: 181 HIPKSLISSDLRYRCCFLWLDVSFPILSEYLSIRVDEMLQLGMFEELAEFYHPDTAETTP 240

Query: 241 YTGIRKAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKG 300
           YTGIRKAIGVPEFD YFKK PP +KNGI+REAFEEAV+AIKRNTHVLAERQI KILKLKG
Sbjct: 241 YTGIRKAIGVPEFDNYFKKYPPGQKNGINREAFEEAVDAIKRNTHVLAERQIGKILKLKG 300

Query: 301 AGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLE 340
           AGWD+HVL ATEAFRAVV+PG+ RNRK+IWEKQIL+PSLRIVKRFLE
Sbjct: 301 AGWDIHVLNATEAFRAVVQPGTGRNRKQIWEKQILKPSLRIVKRFLE 345

[Close]

BLAST of Lsi04G002460 vs. NCBI nr
Match: XP_031741216.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN52088.1 hypothetical protein Csa_008393 [Cucumis sativus])

HSP 1 Score: 549.3 bits (1414), Expect = 2.4e-152
Identity = 284/352 (80.68%), Postives = 308/352 (87.50%), Query Frame = 0


Query: 1   MRVDSTSCSNLMTL----------PSSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAA- 60
           MRV S S  NLMTL           SSSSS SS+KI  L +PFSRR+K Q+VKAIA AA 
Sbjct: 1   MRVGSASSFNLMTLSSSSYSSSSSSSSSSSSSSSKIFILNIPFSRRKKRQQVKAIAAAAT 60

Query: 61  -GKEIKILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDV 120
            GK+  ILVIMGATGCGKSGLSVQLASH+ SEIIN DKMQVYKGLDITTNKIP HERHDV
Sbjct: 61  TGKDQNILVIMGATGCGKSGLSVQLASHYQSEIINCDKMQVYKGLDITTNKIPLHERHDV 120

Query: 121 PHHLLGDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPND 180
           PHHLLGDVDS+H++FTPFH+R+RADNVVSDIA R KLPILVGGSNSFIHA+LVNHFNPN 
Sbjct: 121 PHHLLGDVDSIHQDFTPFHFRLRADNVVSDIASRNKLPILVGGSNSFIHAMLVNHFNPNH 180

Query: 181 DVFSHIPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETA 240
           DVF+  PKS+ISS LRYRCCFLWLDV FPIL+EYLSIRVDEMLQ+GMFEELAEFYDP TA
Sbjct: 181 DVFT--PKSLISSHLRYRCCFLWLDVEFPILAEYLSIRVDEMLQIGMFEELAEFYDPHTA 240

Query: 241 ETTPYTGIRKAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKIL 300
           ET PYTGIRKAIGVPEFD+YF+KNPPSKK+GIDREAFEEAV AIKRNTHVLAERQI KIL
Sbjct: 241 ETMPYTGIRKAIGVPEFDKYFQKNPPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKIL 300

Query: 301 KLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           KLK AGWD++VL ATEAFRAVVEPG+ RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 301 KLKEAGWDINVLNATEAFRAVVEPGTGRNRKEIWEKEILEPSFRIVNRFLKE 350

[Close]

BLAST of Lsi04G002460 vs. NCBI nr
Match: XP_016900206.1 (PREDICTED: adenylate isopentenyltransferase-like, partial [Cucumis melo])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 280/328 (85.37%), Postives = 298/328 (90.85%), Query Frame = 0


Query: 16  SSSSSYSSAKISFLKLPFSRRRKWQRVKAI--AGAAGKEIKILVIMGATGCGKSGLSVQL 75
           SSSSSYSS KI  L +PFSRRRK  +VKAI  A AAGKE KILVIMGATGCGKSGLSVQL
Sbjct: 5   SSSSSYSSTKIFILNIPFSRRRKRHQVKAIAAAAAAGKE-KILVIMGATGCGKSGLSVQL 64

Query: 76  ASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYRVRAD 135
           ASH+ +EIIN DKMQVYKGLDITTNKIP HERHDVPHHLLGDVDS+HE+FTPFH+R RAD
Sbjct: 65  ASHYQAEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGDVDSIHEDFTPFHFRPRAD 124

Query: 136 NVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH-IPKSVISSDLRYRCCFLWL 195
           NVVS+IA R KLPILVGGSNSFIHA+LVN FNPN DVF++ IPK +ISSDLRYRCCFLWL
Sbjct: 125 NVVSNIASRNKLPILVGGSNSFIHAMLVNRFNPNHDVFTNCIPKPLISSDLRYRCCFLWL 184

Query: 196 DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRYFKKN 255
           DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDP TAET PYTGIRKAIGVPEFD+YFKKN
Sbjct: 185 DVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPHTAETMPYTGIRKAIGVPEFDKYFKKN 244

Query: 256 PPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATEAFRAVVEP 315
           PPSKK+GIDREAFEEAV AIKRNTHVLAERQI KILKLK AGWD++VL ATEAFRAVVEP
Sbjct: 245 PPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKILKLKEAGWDINVLNATEAFRAVVEP 304

Query: 316 GSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           G  RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 305 GIGRNRKEIWEKEILEPSFRIVNRFLKE 331

[Close]

BLAST of Lsi04G002460 vs. NCBI nr
Match: KAA0034562.1 (adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK09115.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 502.3 bits (1292), Expect = 3.3e-138
Identity = 247/283 (87.28%), Postives = 263/283 (92.93%), Query Frame = 0


Query: 59  MGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDS 118
           MGATGCGKSGLSVQLASH+ +EIIN DKMQVYKGLDITTNKIP HERHDVPHHLLGDVDS
Sbjct: 1   MGATGCGKSGLSVQLASHYQAEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGDVDS 60

Query: 119 LHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH-IPKS 178
           +HE+FTPFH+R RADNVVS+IA R KLPILVGGSNSFIHA+LVN FNPN DVF++ IPK 
Sbjct: 61  IHEDFTPFHFRPRADNVVSNIASRNKLPILVGGSNSFIHAMLVNRFNPNHDVFTNCIPKP 120

Query: 179 VISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIR 238
           +ISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDP TAET PYTGIR
Sbjct: 121 LISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPHTAETMPYTGIR 180

Query: 239 KAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDM 298
           KAIGVPEFD+YFKKNPPSKK+GIDREAFEEAV AIKRNTHVLAERQI KILKLK AGWD+
Sbjct: 181 KAIGVPEFDKYFKKNPPSKKSGIDREAFEEAVEAIKRNTHVLAERQIGKILKLKEAGWDI 240

Query: 299 HVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           +VL ATEAFRAVVEPG  RNRKEIWEK+ILEPS RIV RFL+E
Sbjct: 241 NVLNATEAFRAVVEPGIGRNRKEIWEKEILEPSFRIVNRFLKE 283

[Close]

BLAST of Lsi04G002460 vs. NCBI nr
Match: XP_022967993.1 (adenylate isopentenyltransferase-like [Cucurbita maxima])

HSP 1 Score: 494.2 bits (1271), Expect = 9.0e-136
Identity = 258/336 (76.79%), Postives = 284/336 (84.52%), Query Frame = 0


Query: 12  MTLPSSSSSYS--SAKISFLKLPFSRRRKWQR-VKAIAGAA----GKEIKILVIMGATGC 71
           MTLPSSSSSY   S++ISFLK P   RR+ +R V AIA AA    GKE KILVI+GATGC
Sbjct: 1   MTLPSSSSSYCSFSSRISFLKAPLIPRRRERRFVNAIAAAATTIGGKE-KILVILGATGC 60

Query: 72  GKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFT 131
           GKSGLSV+L +HFP+EIINSDKMQVY GLDITTNKIP HERHDVPHH+LGDVDS   EFT
Sbjct: 61  GKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDVDSDKGEFT 120

Query: 132 PFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLR 191
           P  +R+RADNVVSDIA R KLPILVGGSNSFIHA+LV HFNPNDDVF+     +ISSDLR
Sbjct: 121 PLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSDLR 180

Query: 192 YRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPE 251
           YRCCFLWLDVAFPIL+EYLSIRVDEMLQLGMFEELAEFY+P TAET  Y+GIRKAIGVPE
Sbjct: 181 YRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYNPTTAETASYSGIRKAIGVPE 240

Query: 252 FDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 311
           FD+YFKK PP++ N I + AF +AVN IK NTHVLAERQI KILKLK AGWD++VL AT 
Sbjct: 241 FDKYFKKYPPARNNKIHKSAFNDAVNEIKLNTHVLAERQIGKILKLKQAGWDINVLNATA 300

Query: 312 AFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           AFRA VEPGSR NR+EIW+ QI EPSLRIV RFLEE
Sbjct: 301 AFRAAVEPGSRMNREEIWKNQIWEPSLRIVNRFLEE 335

[Close]

BLAST of Lsi04G002460 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-86
Identity = 172/335 (51.34%), Postives = 230/335 (68.66%), Query Frame = 0


Query: 13  TLPSSSSSYSSAKISFLKLPFSRRRKWQRVKAI----AGAAGKEIKILVIMGATGCGKSG 72
           T   S SS+SS+  S L     RR+    V +I    + +  ++ K++VI+GATG GKS 
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSR 80

Query: 73  LSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHY 132
           LSV LA+ FPSEIINSDK+QVY+GL+ITTN+I   +R  VPHHLLG ++  H E T   +
Sbjct: 81  LSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEF 140

Query: 133 RVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSHIPKSVISSDLRYRCC 192
           R  A NVV +I  R+K+PI+ GGSNSF+HALL   F+P  D FS     +ISSDLRY CC
Sbjct: 141 RSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSS-GSCLISSDLRYECC 200

Query: 193 FLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRY 252
           F+W+DV+  +L EYL  RVDEM+  GMFEEL+ FYDP  +      GIRKAIGVPEFD Y
Sbjct: 201 FIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGY 260

Query: 253 FKKNPPSKK----NGIDREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 312
           FK+ PP KK    + + + A+++AV+ IKRNT  LA+RQ++KI  LK AGW++  + AT 
Sbjct: 261 FKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATA 320

Query: 313 AFRAV-VEPGSRRNRKEIWEKQILEPSLRIVKRFL 339
           +F+AV ++  S +  +E WE+Q+LEPS++IVKR L
Sbjct: 321 SFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of Lsi04G002460 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 275.8 bits (704), Expect = 4.7e-74
Identity = 146/292 (50.00%), Postives = 193/292 (66.10%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K++VIMGATG GKS LS+ LA+ F  EI+NSDK+Q Y GL +TTN++   ER  VPHHLL
Sbjct: 44  KVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLL 103

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH 173
           G++     E T   +R  A   +S+I  R  LPI+ GGSNSFIHALLV+ F+P    FS 
Sbjct: 104 GELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFS- 163

Query: 174 IPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPE-TAETTP 233
             ++ ISS LRY CCFLW+DV+  +L EYLS RVD+M++ GMFEELA FYDP  +     
Sbjct: 164 -SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 223

Query: 234 YTGIRKAIGVPEFDRYFKKNPPSKKNGID------REAFEEAVNAIKRNTHVLAERQIRK 293
             GI K IG+PEFDRYF   PP +K  +       + A++EAV  IK NT  LA++QI +
Sbjct: 224 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 283

Query: 294 ILKLKGAGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFL 339
           I+KLK +GWD+  L AT +F         R+ +EIW+  +L+ S+++VKRFL
Sbjct: 284 IMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

[Close]

BLAST of Lsi04G002460 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 268.1 bits (684), Expect = 9.8e-72
Identity = 147/296 (49.66%), Postives = 195/296 (65.88%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K++VIMGATG GKS LSV LA HF +EIINSDKMQ Y GL ITTN+    +R  VPHHLL
Sbjct: 6   KMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLL 65

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH 173
           G+++    E T   +RV A   +S+I  RKKLPIL GGSNS+IHALL   ++P +  FS 
Sbjct: 66  GELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSD 125

Query: 174 IPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPY 233
             K  I S+L+Y CCF+W+DV   +L EYLS+R+D M++ GMFEE+AEF+  + A   P 
Sbjct: 126 -HKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP- 185

Query: 234 TGIRKAIGVPEFDRYFK----KNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILK 293
            GI KAIGV EFD Y K     N   K + + +EA+E+AV AIK NT  L + QI KI K
Sbjct: 186 LGIWKAIGVQEFDDYLKMYKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 245

Query: 294 LKGAGWDMHVLKATEAFRAVVE-----PGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           L+ AGWD+  + AT +FR  +       G    +++IW K++LEP ++IV+  L++
Sbjct: 246 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Lsi04G002460 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 261.9 bits (668), Expect = 7.0e-70
Identity = 157/340 (46.18%), Postives = 204/340 (60.00%), Query Frame = 0


Query: 11  LMTLPSSSSSYSSAKISFLKLPFSRRRKWQRVKAIAGAAGKEIKILVIMGATGCGKSGLS 70
           LMTL S   S+SS  +  +   F   R        AG    + K+++I G TG GKS LS
Sbjct: 4   LMTLLSPPLSHSSL-LPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLS 63

Query: 71  VQLASH-FPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLLGDVDSLHEEFTPFHYR 130
           V LA+  FP+EIINSDKMQ+YKG +I TN IP HE+  VPHHLLG       E TP  +R
Sbjct: 64  VDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFR 123

Query: 131 VRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFS-HIPKSVISSDLRYRCC 190
             A   +S +   KKLPI+VGGSNSF HALL   F+P+ D FS     S I SDLRY+CC
Sbjct: 124 SLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCC 183

Query: 191 FLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPYTGIRKAIGVPEFDRY 250
            LW+DV  P+L ++L  RVD+M++ G+ E+LAE YDP   ++    G+RK IGV EFDRY
Sbjct: 184 ILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRY 243

Query: 251 FKKNPPSKKNGI----DREAFEEAVNAIKRNTHVLAERQIRKILKLKGAGWDMHVLKATE 310
           F+  P     GI     + A+EE V  +K  T  L ++Q  KI+KL   GW++  L AT 
Sbjct: 244 FRVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATA 303

Query: 311 AFRAVVE----PGSRRNRKEIWEKQILEPSLRIVKRFLEE 341
           A  A +      G  +N +EIWEK I++ S+ IVK+FL E
Sbjct: 304 AIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Lsi04G002460 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 216.1 bits (549), Expect = 4.4e-56
Identity = 119/286 (41.61%), Postives = 170/286 (59.44%), Query Frame = 0


Query: 54  KILVIMGATGCGKSGLSVQLASHFPSEIINSDKMQVYKGLDITTNKIPFHERHDVPHHLL 113
           K++ +MGATG GKS L++ LA+ FP+EI+NSDK+QVYKGLDI TNK+   E   VPHHLL
Sbjct: 34  KVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHLL 93

Query: 114 GDVDSLHEEFTPFHYRVRADNVVSDIACRKKLPILVGGSNSFIHALLVNHFNPNDDVFSH 173
           G V   +E+FT   ++  A   V  I  R ++PI+ GGSNS+I AL+      ND     
Sbjct: 94  GTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV------ND----- 153

Query: 174 IPKSVISSDLRYRCCFLWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPETAETTPY 233
                +   LRY CCFLW+DV+ P+L  ++S RVD+M+ +G+ +E+   +DP +++ +  
Sbjct: 154 ----CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYS-- 213

Query: 234 TGIRKAIGVPEFDRYFKKNPPSKKNGIDREAFEEAVNAIKRNTHVLAERQIRKILKL-KG 293
            GIR+AIGVPE D + +    +          E A+  IK NT +LA RQ++KI +L K 
Sbjct: 214 AGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQ 273

Query: 294 AGWDMHVLKATEAFRAVVEPGSRRNRKEIWEKQILEPSLRIVKRFL 339
             W+MH + ATE F    E        E W+  +  PS   V++FL
Sbjct: 274 WKWNMHRVDATEVFLRRGEEAD-----EAWDNSVAHPSALAVEKFL 297

The following BLAST results are available for this feature:

BLAST of Lsi04G002460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5GHF7	7.5e-93	60.54	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	2.0e-85	51.34	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9LJL4	6.6e-73	50.00	Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9SB60	1.4e-70	49.66	Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ...	[more]
Q9C6L1	9.9e-69	46.18	Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Lsi04G002460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KR70	1.1e-152	80.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609740 PE=4 SV=1	[more]
A0A1S4DW42	7.4e-152	85.37	adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990777 PE...	[more]
A0A5A7STW3	1.6e-138	87.28	Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1HWS1	4.4e-136	76.79	adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11146736...	[more]
A0A6J1G1X1	1.7e-132	76.16	adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]

BLAST of Lsi04G002460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893291.1	2.4e-160	85.01	adenylate isopentenyltransferase [Benincasa hispida]	[more]
XP_031741216.1	2.4e-152	80.68	adenylate isopentenyltransferase [Cucumis sativus] >KGN52088.1 hypothetical prot...	[more]
XP_016900206.1	1.5e-151	85.37	PREDICTED: adenylate isopentenyltransferase-like, partial [Cucumis melo]	[more]
KAA0034562.1	3.3e-138	87.28	adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK09115.1 ade...	[more]
XP_022967993.1	9.0e-136	76.79	adenylate isopentenyltransferase-like [Cucurbita maxima]	[more]

BLAST of Lsi04G002460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68460.1	1.4e-86	51.34	isopentenyltransferase 1	[more]
AT3G19160.1	4.7e-74	50.00	ATP/ADP isopentenyltransferases	[more]
AT4G24650.1	9.8e-72	49.66	isopentenyltransferase 4	[more]
AT1G25410.1	7.0e-70	46.18	isopentenyltransferase 6	[more]
AT5G19040.1	4.4e-56	41.61	isopentenyltransferase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 54..338 e-value: 5.9E-120 score: 401.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 53..230
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 196..297 e-value: 5.9E-120 score: 401.5
None	No IPR available	PFAM	PF01715	IPPT	coord: 88..165 e-value: 1.0E-17 score: 64.7 coord: 181..285 e-value: 9.1E-11 score: 41.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR11088:SF65	ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATED	coord: 47..339
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 47..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G002460.1	Lsi04G002460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity