Lsi04G002430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G002430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Aspergillus nuclease S(1)
Location	chr04: 2479181 .. 2480907 (-)
RNA-Seq Expression	Lsi04G002430
Synteny	Lsi04G002430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGCCTTTGTATCCTTGGTGTTCATCTTTCCTGTTAGCTTTGGCTGGGGAATTGACGGCCATTTTGCTGTTTGCAGAATCGCTCAGGTTCTTCTCTTCACATTCTTCCACAAACCCATATCTCAAATTTACCTTTTTTGATGAAGAATTTGATCTTTGTTTTGTTGCGACAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTGCAGGAACTGTTGCCAGAGTCTGCAAATGGTGATTTAGCGAGTGTTTGCATTTGAGCTGATAGAGTCAAGTTTCGCTATCATTGGTCCTCTGCACTTCATTTCATTGATACTCCAGATTCCCTTTGCACTTATCAGTATGACAGTTGAGTTACTTCCTCTGTCTCCATCCCTCTGTGTTATCTTTTCTAATTTCTCTGAGCCTTCTTTCTCCCCTGATTCTAAAATTTTGTCATTTTGAATCTCTAAAGGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGTGTTGCAGGCGCCATTAACAATTATACTTCCCAGCTTCTAAGCTACAACACTCAACGTTCTCACTCTGAATGTAATTTCCAAGAAAATGAGAATCTTACAAACTTTGCTTTCAGTGAAGAACGTATGAAATCGCTAATATGCTAAACCTTTTCATCATCAGATAACCTGACTGAAGCACTACTTTTTCTTTCACATTTCATGGGAGACATCCATCAGGTATGATATGAAGTTTATGTACATGTTGTGTGCAATTATTTACTGAAAATGAACTTACGTGTAAAATGTAGCCTCTGCATGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATTGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATGTAAATATAATAACCCAACTTCATCTCTGTGCCTTGGCAATTTCCAGCTTTGCATCAATGAAAATGCAAACTTTCTCGTGATTCTTACAGGTATGGGATAGCAACATAATAGGGACAGCAGAGGGAAAGTTCTATGACTTCAGTGTGGATGGCCTGGTTGATGCAATCCAAACGAATATTACGGTCACATAATTGATAATTCTAAGTCCAAGTCAACTTGAAAAACAAAGCTTGATTCTTATCGTTCCTTAGCAATTCTAATGCATTTTTGTTCCCCTTTGGCTGTTTCTGATCAACAGAAGGAGTGGGCAGATCAAGTTGAAGAATGGGAGAAGTGTAGTTCTAATGAGGTAGCATGCACAGAAATGTATGTGGCACAAGAAACAGTCTATTAATCCTTCAATTTTAGAACACAGCGTTCCAATAATTTACTATCAATTTTCATTGAAATATGCAGATATGCATCAGAAAGTATCCAGGCAGCCTGTGACTGGGCGTACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGGTAGATTGCTTATAAAGCTTGGTTCAGTTGTTCTTTGGGCAGAAGAGTTTTTAACTAAAAAAATTGCGTGCAGAGAAATATTTCTCCTCCCGTGTGCCAGTAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGTATATTTGTTTGACAGACATTCCTCTAGTTTATACTCTTGAGGAAAAGGCCTGCAAGAAATAGTTAGGTTTCAAGTTAGTGCTGAAAAGTTGACCTTAATATGTCAATGTATTCTTGTATAACAATCAGCGCCATGAGGTGAATAAAGTTCTTCCTGGAATATCATTCTCCTTGA

mRNA sequence

ATGGTGGCCTTTGTATCCTTGGTGTTCATCTTTCCTGTTAGCTTTGGCTGGGGAATTGACGGCCATTTTGCTGTTTGCAGAATCGCTCAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTGCAGGAACTGTTGCCAGAGTCTGCAAATGGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGTGTTGCAGGCGCCATTAACAATTATACTTCCCAGCTTCTAAGCTACAACACTCAACGTTCTCACTCTGAATATAACCTGACTGAAGCACTACTTTTTCTTTCACATTTCATGGGAGACATCCATCAGCCTCTGCATGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATTGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATGTATGGGATAGCAACATAATAGGGACAGCAGAGGGAAAGTTCTATGACTTCAGTGTGGATGGCCTGGTTGATGCAATCCAAACGAATATTACGAAGGAGTGGGCAGATCAAGTTGAAGAATGGGAGAAGTGTAGTTCTAATGAGGTAGCATGCACAGAAATATATGCATCAGAAAGTATCCAGGCAGCCTGTGACTGGGCGTACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGAGAAATATTTCTCCTCCCGTGTGCCAGTAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGTATATTTGTTTGACAGACATTCCTCTAGTTTATACTCTTGAGGAAAAGGCCTGCAAGAAATAGTTAGGTTTCAAGTTAGTGCTGAAAAGTTGACCTTAATATGTCAATGTATTCTTGTATAACAATCAGCGCCATGAGGTGAATAAAGTTCTTCCTGGAATATCATTCTCCTTGA

Coding sequence (CDS)

ATGGTGGCCTTTGTATCCTTGGTGTTCATCTTTCCTGTTAGCTTTGGCTGGGGAATTGACGGCCATTTTGCTGTTTGCAGAATCGCTCAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTGCAGGAACTGTTGCCAGAGTCTGCAAATGGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGTGTTGCAGGCGCCATTAACAATTATACTTCCCAGCTTCTAAGCTACAACACTCAACGTTCTCACTCTGAATATAACCTGACTGAAGCACTACTTTTTCTTTCACATTTCATGGGAGACATCCATCAGCCTCTGCATGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATTGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATGTATGGGATAGCAACATAATAGGGACAGCAGAGGGAAAGTTCTATGACTTCAGTGTGGATGGCCTGGTTGATGCAATCCAAACGAATATTACGAAGGAGTGGGCAGATCAAGTTGAAGAATGGGAGAAGTGTAGTTCTAATGAGGTAGCATGCACAGAAATATATGCATCAGAAAGTATCCAGGCAGCCTGTGACTGGGCGTACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGAGAAATATTTCTCCTCCCGTGTGCCAGTAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGTATATTTGTTTGA

Protein sequence

MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANGDCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIFV

Homology

[Close]

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 5.7e-87
Identity = 164/279 (58.78%), Postives = 197/279 (70.61%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           M+  V L++  P   GWG +GH  +C+IAQ+RL + AA AV+ELLPESA G         
Sbjct: 12  MIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWA 71

Query: 61  ----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNT-QR 120
                                       DCKDE+GEKGRCVAGAI NYT+QLLSY T   
Sbjct: 72  DRVKFRYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAAS 131

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHH+WDSNII T
Sbjct: 132 SQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIET 191

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AE   Y+ +++G+VDA++ NIT EWADQV+ WE C + + AC +IYASE IQAACDWAYK
Sbjct: 192 AEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDWAYK 251

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GV+EG TL ++YF SR+P+V  RLAQGGVRLAA LNRIF
Sbjct: 252 GVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.4e-63
Identity = 125/266 (46.99%), Postives = 162/266 (60.90%), Query Frame = 0


Query: 17  WGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------------------- 76
           WG +GH+ VC+IA+S   +    AV++LLP+SA+G                         
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 77  ---------------DCKDEAGEKGRCVAGAINNYTSQLLSYN-TQRSHSEYNLTEALLF 136
                          DC D    + RCV GAI NYT QL+S +    +   YNLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 137 LSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGL 196
           LSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHHVWD+ II +A   +Y+ S+  +
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 197 VDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYF 242
           ++A+Q N+T +W++ V  WE C  N+ AC   YASESI  AC +AY+  + G+TL + YF
Sbjct: 205 IEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYF 264

[Close]

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.6e-59
Identity = 121/283 (42.76%), Postives = 164/283 (57.95%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           +V+ + L  +   +  WG DGH+ VC++A+         AV++LLPES +G         
Sbjct: 5   IVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCSW 64

Query: 61  --------------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYN 120
                                           DC D    K  CV GAI NYT+QL+S +
Sbjct: 65  PDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSAS 124

Query: 121 -TQRSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSN 180
              ++   YNLTEALLFLSH+MGD+HQPLH GF GD GGNTI V+WY  K NLHHVWD+ 
Sbjct: 125 ENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDNM 184

Query: 181 IIGTAEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACD 240
           II +A   +Y+ S+  ++ A+Q  +   W++ V  W+ C  ++ AC  +YASESI  AC 
Sbjct: 185 IIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLACK 244

Query: 241 WAYKGVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           +AY+  + G+TL ++YF SR+PVV  RLAQGG+RLAA LNRIF
Sbjct: 245 YAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIF 287

[Close]

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-58
Identity = 122/261 (46.74%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 17  WGIDGHFAVCRIAQSRLSKAAADAVQELLPESANGD------------------------ 76
           WG  GH+AVC+IAQS   +    AV++LLPESANG+                        
Sbjct: 25  WGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPDEIKKLPQWRWTSALH 84

Query: 77  --------CKDEAGE---KGRCVAGAINNYTSQLLSYN-TQRSHSEYNLTEALLFLSHFM 136
                   C  E      K  CV GAI NYT+QL+S +   +S   YNLTEAL+FLSH+M
Sbjct: 85  FADTPDYKCNYEYSRDCPKDWCVTGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYM 144

Query: 137 GDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGLVDAIQ 196
           GDIHQPLH GF GD GGN I VHWY ++ NLH VWD  II +A   +Y+ S+  ++  +Q
Sbjct: 145 GDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQ 204

Query: 197 TNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYFSSRVP 242
             +   W++ V  WE C  N+ AC   YASESI  AC +AY+  + G+TL + YF SR+P
Sbjct: 205 AKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLACKYAYRNATAGTTLGDYYFVSRLP 264

[Close]

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.1e-57
Identity = 109/191 (57.07%), Postives = 134/191 (70.16%), Query Frame = 0


Query: 52  DCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSEYNLTEALLFLSHFMGDIHQPLHVGF 111
           DC D+ G K  CV GAI N+TSQL  Y    S   YN+TEALLFLSHFMGDIHQP+HVGF
Sbjct: 104 DCHDQHGLKDMCVDGAIQNFTSQLQHYGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 163

Query: 112 TGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGLVDAIQTNITKE-WADQ 171
           T D GGNTID+ WY  K NLHHVWD  II TA  + YD ++D L + ++ NIT   W D 
Sbjct: 164 TSDEGGNTIDLRWYKHKSNLHHVWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDD 223

Query: 172 VEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYFSSRVPVVNLRLAQGG 231
           +  W +C ++ +AC   YASESI+ AC W YKGV  G TL+E+YF++R+P+V  R+ QGG
Sbjct: 224 LSSWTEC-NDLIACPHKYASESIKLACKWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGG 283

Query: 232 VRLAAALNRIF 242
           VRLA  LNR+F
Sbjct: 284 VRLAMILNRVF 293

[Close]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL
Match: A0A5A7SZC9 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001090 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.1e-120
Identity = 223/279 (79.93%), Postives = 232/279 (83.15%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           MVAF+SLVF FPVSFGWGIDGHF VC+IAQSRLSKAAADAVQELLPE A G         
Sbjct: 8   MVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDLASVCIWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKDEAGEKGRCVAGAINNYTSQLL+YNTQ 
Sbjct: 68  DRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYNTQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHHVWDSNII T
Sbjct: 128 SNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVWDSNIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AEGKFYDFSVDGLVDAIQTNIT EWADQVEEWEKC S+EV CTEIYASESIQAACDWAYK
Sbjct: 188 AEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GVSEGSTLAE+YF+SRVPV+NLRLAQGGVRLAAALNRIF
Sbjct: 248 GVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIF 286

[Close]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL
Match: A0A1S3BFG6 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.1e-120
Identity = 223/279 (79.93%), Postives = 232/279 (83.15%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           MVAF+SLVF FPVSFGWGIDGHF VC+IAQSRLSKAAADAVQELLPE A G         
Sbjct: 8   MVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDLASVCIWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKDEAGEKGRCVAGAINNYTSQLL+YNTQ 
Sbjct: 68  DRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYNTQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHHVWDSNII T
Sbjct: 128 SNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVWDSNIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AEGKFYDFSVDGLVDAIQTNIT EWADQVEEWEKC S+EV CTEIYASESIQAACDWAYK
Sbjct: 188 AEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GVSEGSTLAE+YF+SRVPV+NLRLAQGGVRLAAALNRIF
Sbjct: 248 GVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIF 286

[Close]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL
Match: A0A6J1G0B6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.7e-105
Identity = 195/275 (70.91%), Postives = 217/275 (78.91%), Query Frame = 0


Query: 5   VSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------- 64
           V LVFIFPVSFGWG+DGHF +C+IAQSRLS+AAADAVQ LLPE ANG             
Sbjct: 13  VFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGELANVCIWADRIK 72

Query: 65  -------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSE 124
                                    DCKDE G +GRCVAGAINNYTSQLL+Y T+ S S+
Sbjct: 73  FRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQLLTYKTRSSSSQ 132

Query: 125 YNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGK 184
           YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHH+WDSNII TAE K
Sbjct: 133 YNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHIWDSNIIETAEEK 192

Query: 185 FYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSE 242
           FYD SVDGL+DAIQTNITKEW D+VEEWEKC+S ++ACT+IYASESI+AACDWAYKGV+E
Sbjct: 193 FYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIRAACDWAYKGVTE 252

[Close]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL
Match: A0A6J1HU04 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111467455 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.2e-103
Identity = 193/275 (70.18%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 5   VSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------- 64
           V LVFIFPVSFGWG+DGHF +C+IAQSRLS+AAADAVQ LLPE ANG             
Sbjct: 13  VFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGELANVCIWADRIK 72

Query: 65  -------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSE 124
                                    DCKDE G +GRCVAGAINNYTSQLL+Y T+ S S+
Sbjct: 73  FRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQLLTYKTRSSSSQ 132

Query: 125 YNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGK 184
           YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHH+WDSNII TAE K
Sbjct: 133 YNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHIWDSNIIETAEEK 192

Query: 185 FYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSE 242
           FYD SV+GL+DAIQ NITKEW D+VEEW KC+S  +ACT+IYASESI+AACDWAYKGV+E
Sbjct: 193 FYDSSVNGLIDAIQMNITKEWTDEVEEWVKCNSKTIACTDIYASESIRAACDWAYKGVTE 252

[Close]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL
Match: A0A6J1DBY6 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.7e-103
Identity = 196/279 (70.25%), Postives = 213/279 (76.34%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           + A + L FIFPV  GWG+DGH AVC+IAQSRLS+AAADAVQ+LLP+SA+G         
Sbjct: 8   VAALLLLGFIFPVISGWGVDGHAAVCKIAQSRLSRAAADAVQQLLPDSASGDLASVCSWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKD+AG  GRCVAGAINNYTSQLL Y  Q 
Sbjct: 68  DRVKFYYHWSSALHFIDTPDSLCSYQYDRDCKDQAGVIGRCVAGAINNYTSQLLKYQAQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S S+YNLT+ALLFLSHFMGDIHQPLHVGFT DRGGNTIDVHWYTRKQNLHH+WD +II T
Sbjct: 128 SQSKYNLTQALLFLSHFMGDIHQPLHVGFTTDRGGNTIDVHWYTRKQNLHHIWDDSIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AE KFY+FSVDGLVDAIQ NITK W + VEEWEKCSSNEVACTEIYASESIQAACDWAYK
Sbjct: 188 AEEKFYNFSVDGLVDAIQMNITKGWRNHVEEWEKCSSNEVACTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           G SEGS L +KYF SR P+VNLRLAQGGVRLAA LNRIF
Sbjct: 248 GASEGSILEDKYFFSRAPIVNLRLAQGGVRLAAVLNRIF 286

[Close]

BLAST of Lsi04G002430 vs. NCBI nr
Match: XP_008446611.1 (PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis melo var. makuwa] >TYK09118.1 endonuclease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 440.7 bits (1132), Expect = 8.4e-120
Identity = 223/279 (79.93%), Postives = 232/279 (83.15%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           MVAF+SLVF FPVSFGWGIDGHF VC+IAQSRLSKAAADAVQELLPE A G         
Sbjct: 8   MVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDLASVCIWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKDEAGEKGRCVAGAINNYTSQLL+YNTQ 
Sbjct: 68  DRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYNTQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHHVWDSNII T
Sbjct: 128 SNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVWDSNIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AEGKFYDFSVDGLVDAIQTNIT EWADQVEEWEKC S+EV CTEIYASESIQAACDWAYK
Sbjct: 188 AEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GVSEGSTLAE+YF+SRVPV+NLRLAQGGVRLAAALNRIF
Sbjct: 248 GVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIF 286

[Close]

BLAST of Lsi04G002430 vs. NCBI nr
Match: XP_004135088.1 (endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [Cucumis sativus])

HSP 1 Score: 437.6 bits (1124), Expect = 7.1e-119
Identity = 222/279 (79.57%), Postives = 231/279 (82.80%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           +VAF+SLVFIFPVSFGWGIDGHF VC+IAQSRLSKAAADAVQELLPESA G         
Sbjct: 8   IVAFLSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKDEAGEKGRCVAGAINNYTSQLL+YN Q 
Sbjct: 68  DRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYNAQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHH+WDSNII T
Sbjct: 128 SNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AEGKFYDFSVDGLVDAIQTNI  EWADQVEEWEKC S+EV CTEIYASESIQAACDWAYK
Sbjct: 188 AEGKFYDFSVDGLVDAIQTNIKNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GVSEGSTLAEKYF+SRVPV+ LRLAQGGVRLAAALNRIF
Sbjct: 248 GVSEGSTLAEKYFASRVPVLKLRLAQGGVRLAAALNRIF 286

[Close]

BLAST of Lsi04G002430 vs. NCBI nr
Match: XP_038892859.1 (endonuclease 2 [Benincasa hispida])

HSP 1 Score: 433.0 bits (1112), Expect = 1.8e-117
Identity = 221/279 (79.21%), Postives = 226/279 (81.00%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           ++A VSLVFIFPVSFGWGIDGHF VCRIAQSRLS+AA DAVQELLP  ANG         
Sbjct: 8   VMAIVSLVFIFPVSFGWGIDGHFTVCRIAQSRLSQAAEDAVQELLPHYANGDLASVCIWA 67

Query: 61  -----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQR 120
                                        DCKDE GEKGRCVAGAINNYTSQLLSY TQ 
Sbjct: 68  DRIKFRYHWSSALHFIDTPDSLCTYQYDRDCKDEGGEKGRCVAGAINNYTSQLLSYKTQP 127

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNII T
Sbjct: 128 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIET 187

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           +E KFYDFSVDGL+DAIQTNITKEWADQVEEWE C SNEV CTEIYASESIQAACDWAYK
Sbjct: 188 SEKKFYDFSVDGLIDAIQTNITKEWADQVEEWENCGSNEVPCTEIYASESIQAACDWAYK 247

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GV EGSTLAEKYFSSRVP VNLRLAQGGVRLAAALNRIF
Sbjct: 248 GVYEGSTLAEKYFSSRVPEVNLRLAQGGVRLAAALNRIF 286

[Close]

BLAST of Lsi04G002430 vs. NCBI nr
Match: KAG6573953.1 (Endonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 394.4 bits (1012), Expect = 6.9e-106
Identity = 195/269 (72.49%), Postives = 217/269 (80.67%), Query Frame = 0


Query: 5   VSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------- 64
           V LVFIFPVSFGWG+DGHF +C+IAQSRLS+AAADAVQ LLPE ANG             
Sbjct: 13  VFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGELANVCIWADRIK 72

Query: 65  -------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSEYNLTEA 124
                              DCKDE G +GRCVAGAINNYTSQLL+Y T+ S S+YNLTEA
Sbjct: 73  FRYRWSSALHYVNTPESLWDCKDENGVEGRCVAGAINNYTSQLLTYKTRSSSSQYNLTEA 132

Query: 125 LLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSV 184
           LLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHH+WDSNII TAE KFYD SV
Sbjct: 133 LLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHIWDSNIIETAEEKFYDSSV 192

Query: 185 DGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAE 242
           DGL+DAIQTNITKEW D+VEEWEKC+S ++ACT+IYASESI+AACDWAYKGV+EG+TLA 
Sbjct: 193 DGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIRAACDWAYKGVTEGATLAA 252

[Close]

BLAST of Lsi04G002430 vs. NCBI nr
Match: XP_022945222.1 (endonuclease 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 392.1 bits (1006), Expect = 3.4e-105
Identity = 195/275 (70.91%), Postives = 217/275 (78.91%), Query Frame = 0


Query: 5   VSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------- 64
           V LVFIFPVSFGWG+DGHF +C+IAQSRLS+AAADAVQ LLPE ANG             
Sbjct: 13  VFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGELANVCIWADRIK 72

Query: 65  -------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNTQRSHSE 124
                                    DCKDE G +GRCVAGAINNYTSQLL+Y T+ S S+
Sbjct: 73  FRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQLLTYKTRSSSSQ 132

Query: 125 YNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGK 184
           YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHH+WDSNII TAE K
Sbjct: 133 YNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHIWDSNIIETAEEK 192

Query: 185 FYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSE 242
           FYD SVDGL+DAIQTNITKEW D+VEEWEKC+S ++ACT+IYASESI+AACDWAYKGV+E
Sbjct: 193 FYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIRAACDWAYKGVTE 252

[Close]

BLAST of Lsi04G002430 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 322.0 bits (824), Expect = 4.1e-88
Identity = 164/279 (58.78%), Postives = 197/279 (70.61%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           M+  V L++  P   GWG +GH  +C+IAQ+RL + AA AV+ELLPESA G         
Sbjct: 12  MIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWA 71

Query: 61  ----------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYNT-QR 120
                                       DCKDE+GEKGRCVAGAI NYT+QLLSY T   
Sbjct: 72  DRVKFRYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAAS 131

Query: 121 SHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGT 180
           S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHH+WDSNII T
Sbjct: 132 SQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIET 191

Query: 181 AEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYK 240
           AE   Y+ +++G+VDA++ NIT EWADQV+ WE C + + AC +IYASE IQAACDWAYK
Sbjct: 192 AEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDWAYK 251

Query: 241 GVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           GV+EG TL ++YF SR+P+V  RLAQGGVRLAA LNRIF
Sbjct: 252 GVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of Lsi04G002430 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 242.7 bits (618), Expect = 3.1e-64
Identity = 125/266 (46.99%), Postives = 162/266 (60.90%), Query Frame = 0


Query: 17  WGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG------------------------- 76
           WG +GH+ VC+IA+S   +    AV++LLP+SA+G                         
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 77  ---------------DCKDEAGEKGRCVAGAINNYTSQLLSYN-TQRSHSEYNLTEALLF 136
                          DC D    + RCV GAI NYT QL+S +    +   YNLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 137 LSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGL 196
           LSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHHVWD+ II +A   +Y+ S+  +
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 197 VDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYF 242
           ++A+Q N+T +W++ V  WE C  N+ AC   YASESI  AC +AY+  + G+TL + YF
Sbjct: 205 IEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYF 264

[Close]

BLAST of Lsi04G002430 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 228.8 bits (582), Expect = 4.7e-60
Identity = 121/283 (42.76%), Postives = 164/283 (57.95%), Query Frame = 0


Query: 1   MVAFVSLVFIFPVSFGWGIDGHFAVCRIAQSRLSKAAADAVQELLPESANG--------- 60
           +V+ + L  +   +  WG DGH+ VC++A+         AV++LLPES +G         
Sbjct: 5   IVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCSW 64

Query: 61  --------------------------------DCKDEAGEKGRCVAGAINNYTSQLLSYN 120
                                           DC D    K  CV GAI NYT+QL+S +
Sbjct: 65  PDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSAS 124

Query: 121 -TQRSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSN 180
              ++   YNLTEALLFLSH+MGD+HQPLH GF GD GGNTI V+WY  K NLHHVWD+ 
Sbjct: 125 ENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDNM 184

Query: 181 IIGTAEGKFYDFSVDGLVDAIQTNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACD 240
           II +A   +Y+ S+  ++ A+Q  +   W++ V  W+ C  ++ AC  +YASESI  AC 
Sbjct: 185 IIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLACK 244

Query: 241 WAYKGVSEGSTLAEKYFSSRVPVVNLRLAQGGVRLAAALNRIF 242
           +AY+  + G+TL ++YF SR+PVV  RLAQGG+RLAA LNRIF
Sbjct: 245 YAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIF 287

[Close]

BLAST of Lsi04G002430 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-59
Identity = 122/261 (46.74%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 17  WGIDGHFAVCRIAQSRLSKAAADAVQELLPESANGD------------------------ 76
           WG  GH+AVC+IAQS   +    AV++LLPESANG+                        
Sbjct: 25  WGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPDEIKKLPQWRWTSALH 84

Query: 77  --------CKDEAGE---KGRCVAGAINNYTSQLLSYN-TQRSHSEYNLTEALLFLSHFM 136
                   C  E      K  CV GAI NYT+QL+S +   +S   YNLTEAL+FLSH+M
Sbjct: 85  FADTPDYKCNYEYSRDCPKDWCVTGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYM 144

Query: 137 GDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGLVDAIQ 196
           GDIHQPLH GF GD GGN I VHWY ++ NLH VWD  II +A   +Y+ S+  ++  +Q
Sbjct: 145 GDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQ 204

Query: 197 TNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYFSSRVP 242
             +   W++ V  WE C  N+ AC   YASESI  AC +AY+  + G+TL + YF SR+P
Sbjct: 205 AKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLACKYAYRNATAGTTLGDYYFVSRLP 264

[Close]

BLAST of Lsi04G002430 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-59
Identity = 122/261 (46.74%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 17  WGIDGHFAVCRIAQSRLSKAAADAVQELLPESANGD------------------------ 76
           WG  GH+AVC+IAQS   +    AV++LLPESANG+                        
Sbjct: 25  WGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPDEIKKLPQWRWTSALH 84

Query: 77  --------CKDEAGE---KGRCVAGAINNYTSQLLSYN-TQRSHSEYNLTEALLFLSHFM 136
                   C  E      K  CV GAI NYT+QL+S +   +S   YNLTEAL+FLSH+M
Sbjct: 85  FADTPDYKCNYEYSRDCPKDWCVTGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYM 144

Query: 137 GDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVWDSNIIGTAEGKFYDFSVDGLVDAIQ 196
           GDIHQPLH GF GD GGN I VHWY ++ NLH VWD  II +A   +Y+ S+  ++  +Q
Sbjct: 145 GDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQ 204

Query: 197 TNITKEWADQVEEWEKCSSNEVACTEIYASESIQAACDWAYKGVSEGSTLAEKYFSSRVP 242
             +   W++ V  WE C  N+ AC   YASESI  AC +AY+  + G+TL + YF SR+P
Sbjct: 205 AKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLACKYAYRNATAGTTLGDYYFVSRLP 264

The following BLAST results are available for this feature:

BLAST of Lsi04G002430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9G4	5.7e-87	58.78	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL0	4.4e-63	46.99	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	6.6e-59	42.76	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	1.5e-58	46.74	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]
Q9SXA6	2.1e-57	57.07	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]

BLAST of Lsi04G002430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SZC9	4.1e-120	79.93	Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3BFG6	4.1e-120	79.93	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1	[more]
A0A6J1G0B6	1.7e-105	70.91	Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=...	[more]
A0A6J1HU04	1.2e-103	70.18	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111467455 PE=3 SV=1	[more]
A0A6J1DBY6	2.7e-103	70.25	Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV...	[more]

BLAST of Lsi04G002430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446611.1	8.4e-120	79.93	PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis m...	[more]
XP_004135088.1	7.1e-119	79.57	endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [...	[more]
XP_038892859.1	1.8e-117	79.21	endonuclease 2 [Benincasa hispida]	[more]
KAG6573953.1	6.9e-106	72.49	Endonuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022945222.1	3.4e-105	70.91	endonuclease 2 isoform X2 [Cucurbita moschata]	[more]

BLAST of Lsi04G002430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68290.1	4.1e-88	58.78	endonuclease 2	[more]
AT4G21585.1	3.1e-64	46.99	endonuclease 4	[more]
AT4G21600.1	4.7e-60	42.76	endonuclease 5	[more]
AT4G21590.1	1.0e-59	46.74	endonuclease 3	[more]
AT4G21590.2	1.0e-59	46.74	endonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 50..242 e-value: 6.7E-73 score: 247.7
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 17..49 e-value: 3.3E-5 score: 25.7
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 17..240
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 50..241 e-value: 1.1E-57 score: 195.8
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 51..241 coord: 2..52
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 17..241 e-value: 1.32162E-67 score: 207.237
None	No IPR available	PANTHER	PTHR33146:SF2	ENDONUCLEASE 2	coord: 51..241 coord: 2..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G002430.1	Lsi04G002430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds