Lsi04G001120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G001120
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description4-coumarate--CoA ligase
Locationchr04: 1223900 .. 1228217 (+)
RNA-Seq ExpressionLsi04G001120
SyntenyLsi04G001120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCCGCCCATTTCTGATTCCCTCGCTAACGAACCGATTCTTGATCCTTCACTCATCAACGCAAGAACATGTCCGGCGAAGAATCCTGCAATGCGCCGGCCGCTGTGGAATTTCAGCCGCAGAACTCTCCTGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGCTTGACGAAGCTCCTAAATTCTCCACCAGGCCAGACCTCGACACAGCCACTTTCGTCTTATCGCAGTTTCCCACGGCCAAATCGCGAGTTGCTCTCATCGACTCACTCACGAACTCGCGAGTTACTTATGGACAACTCAGTGTGTGGATTCGCTCCCTTGCTTGCGGACTTTATCACGCACTCGGAGTTCGAAAAGGCGACGTGGTGTTCGTTTTATCGTCGAATTCTGTTCTGTATCCGGTGATCTGTTTGGCTGTATTGTCGATTGGTGCGGTGATAACGACAGCCAATCCAATCAATACCGCGTCGGAAATCGGAAAGCAAGTGCGTGATTCAGGTGCGAAACTCGCTATTTCGGCACCGGAGGAGCTTCACAAATTAAGTCCGACTGGAATTCCGACGATCCTAACTACTCGGTCTTCCTATGGCGATGTTTTGTCCGTGGAAGAATTGATAGAATCATGTACCGAATCGTTTGAGCCATTGCTGAAAACAGAAGTAACTCAGTCGGATACGGCGGCGATCTTGTACTCGTCAGGAACGACTGGGACGAGTAAAGGAGTAGTTCTAACTCATTCAAATCTCATCTCTGTTATCGAACTTCTGACCTGGTGCGTCGATTCAACTTCCTCTCAACACGACGTGTTTTTGTGCTTCATTCCCATGTTTCACATCTACGGCCTCGTCTTCTTCGCACTCGGACTGTTCTGCAGAGGAACCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAACGTACAAAATCAATAACATACCGGCAGTACCTCCAGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTTTCATCGCTAAGACGTGTCGGATCTGGAGCTGCACCGCTGGGAAAAGACGTCGAAGAAGCTTTCCGGGAGAAATTTCCATGGGTGGAGCTTCGTCCAGGATATGGCTTAACAGAGAGTACTGGTGCAGCGACTTGGATGGTCACAGACAACGATGCAAAGGCTCATCCAGGTACTAAAATTCATATAAATTCTTAATCCTCTTGCATTTGTGGATCTGAAAATTAGTATTAGCTAGATCGAACAAATGAAATTGTGGTATATCATATCTAACCATTGATTCACATTTTTAACAAGGTTCTTGTGGAATGTTGATGCCGAGTTTTTATGCAAAGATTGTAGACATTGAAACAGGAGAGGGGCTGCCGCCGATGAAGGAAGGCGAGTTGTGGCTGAAGAGTCCAACGATTATGAAAGGATATCTTGGAAACAAGGAAGCAACAGAAGCCACCATGGACGAAGAAGGATGGTTGAAAACAGGAGATCTCGGGTACATAGATGAAGATGGCTTCCTTTACATCGTTGATCGAATCAAAGAACTGATCAAACACAATGGATATCAGGTACAAACAAACACAACAATTCGAATCCTATAAAATTAAGTTAATTCATAAGTTAAATAGTTCATAACTTACAAAATTATTTAATAGGTAATAAAATCTAATAAATCTTAAACTCACACTTTTGAGTCTAACAAGTCTCTTCAATTCCAGAATTGGAATCTTATATCATAACTATTTAGTTATCATTTGAAAACCATTTATTTTTGTCGTGAGTTTTTCGAAAATTAAGCTCAACACTTTTTTACCTCTAAATTGTTTGTTTTATTATCTTTCTGCCAATATTTTAAAAAATTAACAAAAAAAATTTGATGTTTTTTCTTTTTTTTTTGAAAAAAGTAGTTTTAAAAAAAATTATTTTCAAATTTTGGCTTAGAATCCAAACTATTATAAAAAATTGATAGAAAATAGACTTAATTTTCAAAAATTAAATAGTCAATATCTTGATTTTTGGTTTAAGCTTTTAAACATTCTTTCACATATTAATTTTTTTGTGTGTATTATCTATTTTTTAATATGAGTGTTTTCAAAAATCAAGTCAAATTTTAAAAAAAAAAATTAAAATAAGAGTTTTTTGAAAATTTGACCAATCTATCCAAATGTTTAGTTAGGAAAAATGGAAACTATGACAAAGAAGTAGAAAACAAATATAATTTTTAAGAACTAAATGATTATGAAATGTGCCTAAATTTTTAATTTTGTGTTAAGTACATACATAAATTTTAAAATTTTCAAAAGTGAAAACTAGATTGGTAATTTTTTAAAGTTGAGAGACTAAATAGATACCAATTTTAAAAATGTATGGACTAGATTTATACTTTAATCTATAAAATTATCATATCATTATCTGATAATGACAAGAACTTCAAACTTTGAATGAAGGTTGCTCCAGCAGAACTCGAAACAATTCTTTTGAGCCATGCAGAAATTCTGGACGCTGCGGTTATACCGTGAGTTGTTTAGTCTGTTTCTCTTAATTCTCTCTTAAAACTTATAAATCAAAGGCTGAACATTGAGAATGAACTGTCTTTGCAGAATGGAGGATGAGGCCGCTGGACAGATTCCGGTGGCTTGTGTGGTGAAAGCACCCACTTGTAAGCTCACAGAAGAACAAGTCATTCAGTTTGTAGCTTCTCAGGTACTTTCTCTTTGCACCCACTTCTAAATTTTATTGGGCCCTTACCTCCATCTAAATGGGCCTGACCTAGCCCACGCAACCCGTATGCATTTTCCAAACTTTGATTAGGGACTAGGGCGGGGACGGGATGAGGACGGCTAACATAGGCTAATGAATTAAATATTGTAAAAATAGTAGTAGTAAATATAAAAGGTCAAAAGAAAAAGAAAGAAAACAGAAAAGGAAAAAACTCAAGGTTTGAGGTTTGAATCCCGATTCCTTCAGTCCCATTGGGTAACTATTTCGTTTTTTATTTTTGTTTTGAAAACTAAGTCTATTTTCTCTTCATTTCTTACATTGATTTGCATCTCTATTAAGTACAATAGTTGAATTCTTTGTCAAATTCTAAAAACAAAAAAAATATTTAAAATCTACTTTTTTTAGTTTTCAAATTTTTGCTTAGTTTTTTAAATCATTGGTAAAAAGTAGATAACAAAGGAATAAATTTGTCTATAAACTTAATTTTCAAAAACAAAAACAAAAAACGAAATAGTTACAAAACGAAGCCTTAATTTTTAGAGAAATAACAATTTAATCATTAAATTTTATAAATTTGCAATGATTTAGTCATTTATAGAAATAAATCTCATAAAAAAATTTCTATTAAAAAAATCATCAAAATTAGTTGTGAATTTTTAAAAGTGTGTTAATCGAGTATAGCTCAACTAACATGAACACATATCTCCCACACCACATATTATCTAAAAGAAAAAAAAATATATATTTACTTTTAATTTTGTGCCTAATTATTTTCGGACAAATTCAATTTCTCTAAAAATTAATTGATTTTAATTAGATATAAATTTGGATTTTATGGTTAATGAAACCTTAAATTTTTAAATTTGTGTCTAATAGATTCGAATTTTAAAAAATTTAAAAGCTCAAAAACTAAATTTGCAAATTTAGTTGAATTACATTCTCGCCCTTACACTACTAAACTCCAAAGTAATATATTATCATGTTCATGATAATGGATCTAATCATAAATTGAAAAGATTTGTTGAAATTATGGATGACTAATATTCTCACACACACATATATAAAGAATGACATGTAATGCAATAAAAGGATTGATTTTATTATGTGAGAAAATAAATGAAGTATAAAAAAAAATTCAGGTGGCACCTTACAGGAAGATAAGAGGAGTGAGATTCATTAGTGTCATTCCAAGGTCACTTGCTGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAAAAACAGCCTCAACATAAAATTATTATTGATAGTTATATCTATGGTATATGATGGGCAATAATTTTGAACCATCTCTCTACCTCCTCAAAGAGAGAGACAACAAATGCATTGTAACTATCCTATAATAATTAATAGCAAAAATTCACCAAAAGATTCCAAGTGTATGGTTGTAAATTGATCTGTCTTTCATCAGCTCAAAAATAAATTTTTCAATTTTTACCTTAAGTAGATTATATGGTAATGTTAAAATGATTCTAAAGTTGCCTCCATCTTCTTGTAGTTTTCTCTAATCACAGAAAAAAATAAAAA

mRNA sequence

ATCCCGCCCATTTCTGATTCCCTCGCTAACGAACCGATTCTTGATCCTTCACTCATCAACGCAAGAACATGTCCGGCGAAGAATCCTGCAATGCGCCGGCCGCTGTGGAATTTCAGCCGCAGAACTCTCCTGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGCTTGACGAAGCTCCTAAATTCTCCACCAGGCCAGACCTCGACACAGCCACTTTCGTCTTATCGCAGTTTCCCACGGCCAAATCGCGAGTTGCTCTCATCGACTCACTCACGAACTCGCGAGTTACTTATGGACAACTCAGTGTGTGGATTCGCTCCCTTGCTTGCGGACTTTATCACGCACTCGGAGTTCGAAAAGGCGACGTGGTGTTCGTTTTATCGTCGAATTCTGTTCTGTATCCGGTGATCTGTTTGGCTGTATTGTCGATTGGTGCGGTGATAACGACAGCCAATCCAATCAATACCGCGTCGGAAATCGGAAAGCAAGTGCGTGATTCAGGTGCGAAACTCGCTATTTCGGCACCGGAGGAGCTTCACAAATTAAGTCCGACTGGAATTCCGACGATCCTAACTACTCGGTCTTCCTATGGCGATGTTTTGTCCGTGGAAGAATTGATAGAATCATGTACCGAATCGTTTGAGCCATTGCTGAAAACAGAAGTAACTCAGTCGGATACGGCGGCGATCTTGTACTCGTCAGGAACGACTGGGACGAGTAAAGGAGTAGTTCTAACTCATTCAAATCTCATCTCTGTTATCGAACTTCTGACCTGGTGCGTCGATTCAACTTCCTCTCAACACGACGTGTTTTTGTGCTTCATTCCCATGTTTCACATCTACGGCCTCGTCTTCTTCGCACTCGGACTGTTCTGCAGAGGAACCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAACGTACAAAATCAATAACATACCGGCAGTACCTCCAGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTTTCATCGCTAAGACGTGTCGGATCTGGAGCTGCACCGCTGGGAAAAGACGTCGAAGAAGCTTTCCGGGAGAAATTTCCATGGGTGGAGCTTCGTCCAGGATATGGCTTAACAGAGAGTACTGGTGCAGCGACTTGGATGGTCACAGACAACGATGCAAAGGCTCATCCAGGTTCTTGTGGAATGTTGATGCCGAGTTTTTATGCAAAGATTGTAGACATTGAAACAGGAGAGGGGCTGCCGCCGATGAAGGAAGGCGAGTTGTGGCTGAAGAGTCCAACGATTATGAAAGGATATCTTGGAAACAAGGAAGCAACAGAAGCCACCATGGACGAAGAAGGATGGTTGAAAACAGGAGATCTCGGAATGGAGGATGAGGCCGCTGGACAGATTCCGGTGGCTTGTGTGGTGAAAGCACCCACTTGTAAGCTCACAGAAGAACAAGTCATTCAGTTTGTAGCTTCTCAGGTGGCACCTTACAGGAAGATAAGAGGAGTGAGATTCATTAGTGTCATTCCAAGGTCACTTGCTGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAAAAACAGCCTCAACATAAAATTATTATTGATAGTTATATCTATGGTATATGATGGGCAATAATTTTGAACCATCTCTCTACCTCCTCAAAGAGAGAGACAACAAATGCATTGTAACTATCCTATAATAATTAATAGCAAAAATTCACCAAAAGATTCCAAGTGTATGGTTGTAAATTGATCTGTCTTTCATCAGCTCAAAAATAAATTTTTCAATTTTTACCTTAAGTAGATTATATGGTAATGTTAAAATGATTCTAAAGTTGCCTCCATCTTCTTGTAGTTTTCTCTAATCACAGAAAAAAATAAAAA

Coding sequence (CDS)

ATGTCCGGCGAAGAATCCTGCAATGCGCCGGCCGCTGTGGAATTTCAGCCGCAGAACTCTCCTGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGCTTGACGAAGCTCCTAAATTCTCCACCAGGCCAGACCTCGACACAGCCACTTTCGTCTTATCGCAGTTTCCCACGGCCAAATCGCGAGTTGCTCTCATCGACTCACTCACGAACTCGCGAGTTACTTATGGACAACTCAGTGTGTGGATTCGCTCCCTTGCTTGCGGACTTTATCACGCACTCGGAGTTCGAAAAGGCGACGTGGTGTTCGTTTTATCGTCGAATTCTGTTCTGTATCCGGTGATCTGTTTGGCTGTATTGTCGATTGGTGCGGTGATAACGACAGCCAATCCAATCAATACCGCGTCGGAAATCGGAAAGCAAGTGCGTGATTCAGGTGCGAAACTCGCTATTTCGGCACCGGAGGAGCTTCACAAATTAAGTCCGACTGGAATTCCGACGATCCTAACTACTCGGTCTTCCTATGGCGATGTTTTGTCCGTGGAAGAATTGATAGAATCATGTACCGAATCGTTTGAGCCATTGCTGAAAACAGAAGTAACTCAGTCGGATACGGCGGCGATCTTGTACTCGTCAGGAACGACTGGGACGAGTAAAGGAGTAGTTCTAACTCATTCAAATCTCATCTCTGTTATCGAACTTCTGACCTGGTGCGTCGATTCAACTTCCTCTCAACACGACGTGTTTTTGTGCTTCATTCCCATGTTTCACATCTACGGCCTCGTCTTCTTCGCACTCGGACTGTTCTGCAGAGGAACCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAACGTACAAAATCAATAACATACCGGCAGTACCTCCAGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTTTCATCGCTAAGACGTGTCGGATCTGGAGCTGCACCGCTGGGAAAAGACGTCGAAGAAGCTTTCCGGGAGAAATTTCCATGGGTGGAGCTTCGTCCAGGATATGGCTTAACAGAGAGTACTGGTGCAGCGACTTGGATGGTCACAGACAACGATGCAAAGGCTCATCCAGGTTCTTGTGGAATGTTGATGCCGAGTTTTTATGCAAAGATTGTAGACATTGAAACAGGAGAGGGGCTGCCGCCGATGAAGGAAGGCGAGTTGTGGCTGAAGAGTCCAACGATTATGAAAGGATATCTTGGAAACAAGGAAGCAACAGAAGCCACCATGGACGAAGAAGGATGGTTGAAAACAGGAGATCTCGGAATGGAGGATGAGGCCGCTGGACAGATTCCGGTGGCTTGTGTGGTGAAAGCACCCACTTGTAAGCTCACAGAAGAACAAGTCATTCAGTTTGTAGCTTCTCAGGTGGCACCTTACAGGAAGATAAGAGGAGTGAGATTCATTAGTGTCATTCCAAGGTCACTTGCTGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAA

Protein sequence

MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKTGDLGMEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDLVSQFKQQQLLSKL
Homology
[Close]
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.3e-96
Identity = 214/539 (39.70%), Postives = 310/539 (57.51%), Query Frame = 0 

Query: 23  GYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQL 82
           GY  + ++++S  +  + P+      +D  TF+ S+      ++A ID+ T   +T+ QL
Sbjct: 14  GYCKSNSIFYSKREPVQLPQ---NHSVDVTTFISSR--AHHGKIAFIDAATGRHLTFPQL 73

Query: 83  SVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIG 142
              + S+A  L  A+G+RKGDV+ +LS NS+ +PV+CLAV+S+GA+ITT NP+NT  EI 
Sbjct: 74  WRAVDSVATCL-SAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIA 133

Query: 143 KQVRDSGAKLAISAPEELHKLSPTGIPTIL---TTRSSYGDVLS-VEELIESCTESFEP- 202
           KQ+ DS   LA + P+ + K++ + +P ++     +SS    L+ V  L E   +   P 
Sbjct: 134 KQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPN 193

Query: 203 LLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIP 262
            +   V Q DTA +LYSSGTTG SKGVV +H NLI++++ +     +   +H  F+C +P
Sbjct: 194 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEH-TFICTVP 253

Query: 263 MFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSG-- 322
           MFHIYGL  FA+GL   G+T V++ +F    M+ AIE Y+   +P VPP+++ L+K+   
Sbjct: 254 MFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANH 313

Query: 323 --GGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAK 382
                D SSL+ V SG APL K+V E F E +P V +  GYGLTESTG         +++
Sbjct: 314 IRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESR 373

Query: 383 AHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEG 442
            + G+ GML PS  AKIV+ ETGE L   + GELWL+ PTIMKGY  N+EAT +T+D EG
Sbjct: 374 RY-GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEG 433

Query: 443 WLKTGDLGMEDE-----------------------------------------------A 502
           WL+TGDL   DE                                                
Sbjct: 434 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKE 493

Query: 503 AGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDLV 506
           AGQ P+A VV+     L+E  V+ F+A  VAPY++IR V F++ IP++ +GKILRKDL+
Sbjct: 494 AGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLI 544

[Close]
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 352.1 bits (902), Expect = 1.1e-95
Identity = 212/542 (39.11%), Postives = 303/542 (55.90%), Query Frame = 0 

Query: 19  NSPGGY-NVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRV 78
           NS  G+ N N+  Y     +   P     P LD  TF+ SQ    + R+A ID+ T   +
Sbjct: 5   NSRSGFCNSNSTFYSKRTPIPLPP----NPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64

Query: 79  TYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINT 138
           T+ +L   + S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65  TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124

Query: 139 ASEIGKQVRDSGAKLAISAPEELHKLSPTG--IPTILTTRSSYGDVLSVEELIESC-TES 198
           ++EI KQ++DS   LA +  + L K+S     +P +L        V  V  L+E    E 
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184

Query: 199 FEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLC 258
               +K  V Q DTA +LYSSGTTG SKGV+ +H NLI++++ +     S   +   F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244

Query: 259 FIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKS 318
            +PMFHIYGL  FA GL   G+T +++ +F    M+ AI  Y+  ++P VPP+++ +V  
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304

Query: 319 G----GGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDN 378
                   D SS+  V  G APL K+V E F EK+P V++  GYGLTESTG      T  
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364

Query: 379 DAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMD 438
           +++ + G+ G L  S   +IVD  TG+ L P + GELWLK P+IMKGY  N+EAT +T+D
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424

Query: 439 EEGWLKTGDL-----------------------------------------------GME 498
            EGWL+TGDL                                                  
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484

Query: 499 DEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKD 506
           D+  GQ P+A VV+     L+E+ +++FVA QVAPY++IR V F+S IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537

[Close]
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.7e-94
Identity = 204/539 (37.85%), Postives = 296/539 (54.92%), Query Frame = 0 

Query: 30  VYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSL 89
           +Y SL      PK    P+    +F+     +  S++A+ DS T   +T+ QL   +  L
Sbjct: 11  IYRSLRPTLVLPK---DPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 70

Query: 90  ACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSG 149
           A G +H LG+RK DVV + + NS  +P+  LAV +IG V TTANP+ T +E+ KQ++DS 
Sbjct: 71  AHG-FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSN 130

Query: 150 AKLAISAPEELHKLSPTGIPTIL--------TTRSSYGDVLSVEELIESCTESFEPLLKT 209
            K+ IS  +   K+    +P +L            S   +LS + ++E  +E        
Sbjct: 131 PKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFV 190

Query: 210 EVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIPMFHI 269
           E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++T   D     H VFLCF+PMFH+
Sbjct: 191 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHV 250

Query: 270 YGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSG--GGSD 329
           +GL         RG   V M RF  + ++  IE +++ ++  VPPV L L K       D
Sbjct: 251 FGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFD 310

Query: 330 FSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPGSC 389
            SSL+ +GSGAAPLGKD+ E      P V L  GYG+TE+ G  + +      K + GS 
Sbjct: 311 LSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVS-VEDPRLGKRNSGSA 370

Query: 390 GMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKTGD 449
           GML P   A+IV +ETG+  PP ++GE+W++ P +MKGYL N +AT+ T+D++ W+ TGD
Sbjct: 371 GMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGD 430

Query: 450 LG-----------------------------------------------MEDEAAGQIPV 509
           LG                                                 DE AG++P+
Sbjct: 431 LGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPI 490

Query: 510 ACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDLVSQFKQQ 512
           A VV++P   +TE+ + +F+A QVAPY+++R V FIS++P+S AGKILR++LV Q + +
Sbjct: 491 AFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543

[Close]
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 1.1e-92
Identity = 210/545 (38.53%), Postives = 305/545 (55.96%), Query Frame = 0 

Query: 18  QNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRV 77
           QN P  ++  T +Y S       P     P+LD  + + S         ALIDSLT   +
Sbjct: 19  QNPPFWFSSKTGIYTSKFPSLHLP---VDPNLDAVSALFSH--KHHGDTALIDSLTGFSI 78

Query: 78  TYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINT 137
           ++ +L + ++S+A G+YH LGVR+GDVV ++  NSV +P+I L+++S+GA++TT NP ++
Sbjct: 79  SHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSS 138

Query: 138 ASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVE--ELIESCTESF 197
             EI KQV +    LA ++ E + KLS  G+  I  + S   D + +E  +      ESF
Sbjct: 139 LGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESF 198

Query: 198 EPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHD----- 257
             + K  + Q D AAI+YSSGTTG SKGV+LTH NLI+ +EL    V   +SQ++     
Sbjct: 199 GFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELF---VRFEASQYEYPGSS 258

Query: 258 -VFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVIL 317
            V+L  +P+ HIYGL  F +GL   G+T V+M+RF+   +++ IE +KI + P VPP+++
Sbjct: 259 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 318

Query: 318 GLVKSGG---GSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWM 377
            L K      G  F SL++V SGAAPL +   E F +  P V+L  GYG+TEST   T  
Sbjct: 319 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT-R 378

Query: 378 VTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATE 437
             +++  +   S G+L P+  AK+VD  +G  LPP   GELW++ P +MKGYL N +AT+
Sbjct: 379 GFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQ 438

Query: 438 ATMDEEGWLKTGDLGMED------------------------------------------ 497
            ++ E+ WL+TGD+   D                                          
Sbjct: 439 MSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAV 498

Query: 498 -----EAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKI 505
                E  G+IPVA VV+     L+EE VI +VASQVAPYRK+R V  ++ IP+S  GKI
Sbjct: 499 TAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 554

[Close]
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match: Q10S72 (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 4.1e-90
Identity = 203/516 (39.34%), Postives = 293/516 (56.78%), Query Frame = 0 

Query: 47  PDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGL-YHALGVRKGDVV 106
           P++D  +F+ S+       VAL+D+ T  R+T+ +L   +   A  L  H + +RKG V 
Sbjct: 35  PEVDVVSFLASR--RHSGVVALVDAATGRRITFTELWRAVAGAASALAAHPVSLRKGHVA 94

Query: 107 FVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSP 166
            +LS NSV +PV  LA +S+GAV+TTANP+NT +EI KQV D+   LA +  E L KL  
Sbjct: 95  LILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAEIAKQVADARPVLAFTTRELLPKLPR 154

Query: 167 TGIPTILTTRSSY--GD------VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGT 226
                ++   S+   GD      V ++EE+  S T       K  VTQ D A +LYSSGT
Sbjct: 155 AHDLRVVLLESARLPGDSSDPRIVATIEEI--SATTPDPARRKDRVTQDDPATLLYSSGT 214

Query: 227 TGTSKGVVLTHSNLISVIE-LLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGT 286
           TG SKGVV TH +LIS+++ ++T      S + + FLC +PMFH+YGLV FA GL   G 
Sbjct: 215 TGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTETFLCTVPMFHVYGLVAFATGLLGCGA 274

Query: 287 TTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKD 346
           T V++ ++    M+ +I  Y +  +P VPP+++ +V          +R+V SG APLGK+
Sbjct: 275 TVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAMVAHPKPLPLGQMRKVLSGGAPLGKE 334

Query: 347 VEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETG 406
           + E FREK+P VE+  GYGLTEST       +  +++ + G+ G+L P+  AKIVD ++G
Sbjct: 335 LIEGFREKYPQVEILQGYGLTESTAIGASTDSAEESRRY-GTAGLLSPNTEAKIVDPDSG 394

Query: 407 EGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKTGDLGMEDE----------- 466
           E LP  + GELW++ P +MKGY  N EAT++T+  +GWLKTGDL   DE           
Sbjct: 395 EALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTPDGWLKTGDLCYIDEDGYLFVVDRLK 454

Query: 467 ------------------------------------AAGQIPVACVVKAPTCKLTEEQVI 506
                                                 GQ P+A +V+     L+E +V+
Sbjct: 455 ELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPDREVGQFPMAYIVRKKGSNLSEREVM 514

[Close]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match: A0A1S3BFZ3 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.4e-257
Identity = 470/566 (83.04%), Postives = 491/566 (86.75%), Query Frame = 0 

Query: 1   MSGEESCN-APAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQF 60
           MS EESC+  PAA+EFQP+NSPGGYNVNTAVYHSLI  DEAP  STR DLDTAT+VLSQF
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQF 60

Query: 61  PTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVIC 120
           PTA+SRVALIDS+T+ RVTYGQL V IRSLACGLYHALGVRKGDVVFVLS+N VLYPVIC
Sbjct: 61  PTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVIC 120

Query: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYG 180
           LAVLSIGAVITTANPINTASEIGKQVRDSGAKLA+SAPEEL KL PTGIPTILTTRSS+ 
Sbjct: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFS 180

Query: 181 DVLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240
           D LSVEELIESC+ES EPL KTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT
Sbjct: 181 DALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240

Query: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKIN 300
           WCVDSTSSQHDVFLCFIPMFHIYGLVFF LGLFCRG TTVLMQRFNFQSMIDAIE YKIN
Sbjct: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKIN 300

Query: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360
           NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES
Sbjct: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360

Query: 361 TGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYL 420
           TGAATWM+TD DAKAHPGSCGMLMPSFY KIVD+ETGEGLPPMK+GELWLKSPTIMK YL
Sbjct: 361 TGAATWMITDKDAKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYL 420

Query: 421 GNKEATEATMDEEGWLKTGDLG-------------------------------------- 480
           GN+EATEAT+DEEGWLKTGDLG                                      
Sbjct: 421 GNREATEATLDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHT 480

Query: 481 ---------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIP 518
                    +EDEAAGQIPVACVVKAP+C LTE+QVIQFVASQVAPY+K+RGVRFIS IP
Sbjct: 481 EILDAAVIPIEDEAAGQIPVACVVKAPSCVLTEQQVIQFVASQVAPYKKVRGVRFISAIP 540

[Close]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match: A0A0A0KRI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.1e-250
Identity = 457/553 (82.64%), Postives = 480/553 (86.80%), Query Frame = 0 

Query: 13  VEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSL 72
           VEFQPQ+SPGGYNVNTAVYHSL+ LDEAP  STR DLDTAT+VLSQFPTA+SRVALIDS+
Sbjct: 7   VEFQPQSSPGGYNVNTAVYHSLLHLDEAPVISTRSDLDTATYVLSQFPTAESRVALIDSV 66

Query: 73  TNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTA 132
           T+ RVTYGQLSV IRSLACGLYHALGVRKGDVVFVLS+N VLYPVICLAVLSIGAVITTA
Sbjct: 67  TSLRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVLSIGAVITTA 126

Query: 133 NPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVEELIESCT 192
           NP+NT SEIGKQVRDSGAKLA+SAPEEL KLSPTGIPTILTTRSSYGD LSVEELIESC+
Sbjct: 127 NPMNTESEIGKQVRDSGAKLAVSAPEELQKLSPTGIPTILTTRSSYGDALSVEELIESCS 186

Query: 193 E-SFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDV 252
           E S EPL K EVT SDTAAILYSSGTTGTSKGVVLTHSNLISVIE+LTWCVDSTSSQHDV
Sbjct: 187 ESSSEPLPKAEVTPSDTAAILYSSGTTGTSKGVVLTHSNLISVIEILTWCVDSTSSQHDV 246

Query: 253 FLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGL 312
           FLCFIPMFHIYGLVFF LGLF RG TTVLM RFNFQSMIDAIE YKINNIPAVPPVILGL
Sbjct: 247 FLCFIPMFHIYGLVFFGLGLFRRGITTVLMPRFNFQSMIDAIEKYKINNIPAVPPVILGL 306

Query: 313 VKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDND 372
           VKS GGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWM+TD D
Sbjct: 307 VKSDGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMITDKD 366

Query: 373 AKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDE 432
           AKAHPGSCGMLMPSFY KIVD+ETGEGLPPMK+GELWLKSPTIMK YLGN+EATEAT+DE
Sbjct: 367 AKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYLGNREATEATIDE 426

Query: 433 EGWLKTGDLG-----------------------------------------------MED 492
           EGWLKTGDLG                                               +ED
Sbjct: 427 EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTEILDAAVIPIED 486

Query: 493 EAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDL 518
           EAAGQIPVACVVKAP+C+L+E+QVIQFVASQVAPY+K+RGVRFIS IPRSLAGKILRKDL
Sbjct: 487 EAAGQIPVACVVKAPSCELSEQQVIQFVASQVAPYKKVRGVRFISAIPRSLAGKILRKDL 546

[Close]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match: A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.9e-250
Identity = 457/564 (81.03%), Postives = 484/564 (85.82%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFP 60
           MS EESC APAAVEF+P NSPGGY+VNTAVYHS +QLDEAPK ST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICL 120
           TA+SRVALIDS+T+SRVTYG+LS   RSLA GLYHALGVRKGDVVFVLS+NSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGD 180
           AVLSIGAVITTANP+NTASEIGKQV DS AKLAISAPEELHKLSPTGIPTILTT+ SYGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180

Query: 181 VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
           VLSVEELIESC ESFE + +T++ QSD AAILYSSGTTGTSKGVVLTHSNLISVIELLTW
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINN 300
            VD++SSQ DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQSMIDAIETYK+NN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLG 420
           GAAT M+TD DA+AHPGSCGMLMP F AKIVDIETGEGLPPMKEGE+WLKSPTIMK YL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDEEGWLKTGDLG--------------------------------------- 480
           NKEATEATMD+EGWLKTGDLG                                       
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 --------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPR 518
                    EDEAAGQIPVACVVKAP+CKLTEEQVIQFVASQVA Y+KIRGVRF+S IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

[Close]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match: A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.9e-248
Identity = 456/564 (80.85%), Postives = 483/564 (85.64%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFP 60
           MS EESC APAAVEF+P NSPGGY+VNTAVYHSL+Q DEAPK ST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICL 120
           TA+SRVALIDS+T+SRVTYG+LS   RSLA GLYHALGVRKGDVVFVLS+NSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGD 180
           AVLSIGAVITTANP+NTASEIGKQV DS AKLAISAPEELHKLSPTG+PTILTTR SYGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180

Query: 181 VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
           VLSVEELIESC ESFE + KT++ QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINN 300
            VD++SSQ DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQSMIDAIETYK+NN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLG 420
           GAAT M+TD DA+AHPGSCGMLMP F AKIVDIETGEGLPPMKEGE+WLKSPTIMK YL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDEEGWLKTGDLG--------------------------------------- 480
           NKEATEATMD+EGW+KTGDLG                                       
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 --------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPR 518
                    EDEAAGQIPVACVVKAP+CKLTEEQVIQFVASQVA Y+KIRGVRF+S IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

[Close]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match: A0A6J1CEK1 (4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 444/571 (77.76%), Postives = 481/571 (84.24%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSP------GGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATF 60
           MSGEES  APAA E+  QN+P      GGY+VN AVYHSL++ DEA K S+RPDLDTATF
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEARKISSRPDLDTATF 60

Query: 61  VLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVL 120
           VLSQFP A+SRVALIDS+T+SRVTYGQLS  IRSLACGL+HALGVRKGDVVFVLS+NS+L
Sbjct: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120

Query: 121 YPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTT 180
           YPVICLAVLS+GAVITTANP+NTASEI KQVRDSGAKLA+SAPEE+HKL+PTGIPTILTT
Sbjct: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180

Query: 181 RSSYG-DVLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           R S G DVLSVEELIESC +S E L  TEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAI 300
           VIELLT  VD++SS++DVFLCFIPMFHIYGLVFF LGLFCRG T +LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300

Query: 301 ETYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPG 360
           E YK+NNIPAVPPVILGLVKSGGGSD SSLRR+GSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAAT ++TD DAK HPGSCGMLMP F AKIVDIETGEGLPPMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420

Query: 421 IMKGYLGNKEATEATMDEEGWLKTGDLG-------------------------------- 480
           IMKGYLGN+EATEATMDEEGWLKTGDLG                                
Sbjct: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ---------------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVR 518
                          MEDEAAGQIPVACVVKA T +LTE+QVIQFVASQVAPY+K+RGV+
Sbjct: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540

[Close]
BLAST of Lsi04G001120 vs. NCBI nr
Match: XP_038891768.1 (4-coumarate--CoA ligase-like 5 [Benincasa hispida])

HSP 1 Score: 918.7 bits (2373), Expect = 2.3e-263
Identity = 480/564 (85.11%), Postives = 497/564 (88.12%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFP 60
           MSGEESC APAAVEFQPQNSPGGYNV TAVYHSLIQLDEAP  STR DL+TATFVLSQFP
Sbjct: 1   MSGEESCTAPAAVEFQPQNSPGGYNVKTAVYHSLIQLDEAPIISTRQDLNTATFVLSQFP 60

Query: 61  TAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICL 120
           TA+SRVALIDS+T+SRVTYGQLSV IRSLACGLYHALGVRKGDVVFVLS+NSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGD 180
           AV+SIGAVITTANPINTASEIGKQVRDSGAKLA+SAPEELHKLSPTGIPTILTTRSSYGD
Sbjct: 121 AVMSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELHKLSPTGIPTILTTRSSYGD 180

Query: 181 VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
            LSVEELIESC+ESFEPL K EVTQSD AAILYSSGTTGTSKGVVLTHSN+ISVIELLTW
Sbjct: 181 TLSVEELIESCSESFEPLPKIEVTQSDPAAILYSSGTTGTSKGVVLTHSNIISVIELLTW 240

Query: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINN 300
           CVDSTSSQHDVFLCFIPMFHIYGLVFF LGLFCRG TTVLMQRFNFQSMI AIE +KINN
Sbjct: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIAAIEKHKINN 300

Query: 301 IPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKSG GSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSGDGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLG 420
           GAATWM+TD DAKAHPGSCGMLMPSF+AKIVD+ETGEG+ PMKEGELWLKSPTIMKGYLG
Sbjct: 361 GAATWMITDKDAKAHPGSCGMLMPSFFAKIVDVETGEGVAPMKEGELWLKSPTIMKGYLG 420

Query: 421 NKEATEATMDEEGWLKTGDLG--------------------------------------- 480
           NKEATEAT+DEEGWLKTGDLG                                       
Sbjct: 421 NKEATEATIDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 --------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPR 518
                   MEDEAAGQIPVACVVKAPT KLTEEQVIQFVASQVA Y+K+RGVRFIS IPR
Sbjct: 481 ILDAAVIPMEDEAAGQIPVACVVKAPTSKLTEEQVIQFVASQVAAYKKVRGVRFISSIPR 540

[Close]
BLAST of Lsi04G001120 vs. NCBI nr
Match: XP_008446808.1 (PREDICTED: 4-coumarate--CoA ligase-like 5 isoform X1 [Cucumis melo])

HSP 1 Score: 897.1 bits (2317), Expect = 7.0e-257
Identity = 470/566 (83.04%), Postives = 491/566 (86.75%), Query Frame = 0 

Query: 1   MSGEESCN-APAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQF 60
           MS EESC+  PAA+EFQP+NSPGGYNVNTAVYHSLI  DEAP  STR DLDTAT+VLSQF
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQF 60

Query: 61  PTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVIC 120
           PTA+SRVALIDS+T+ RVTYGQL V IRSLACGLYHALGVRKGDVVFVLS+N VLYPVIC
Sbjct: 61  PTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVIC 120

Query: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYG 180
           LAVLSIGAVITTANPINTASEIGKQVRDSGAKLA+SAPEEL KL PTGIPTILTTRSS+ 
Sbjct: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFS 180

Query: 181 DVLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240
           D LSVEELIESC+ES EPL KTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT
Sbjct: 181 DALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240

Query: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKIN 300
           WCVDSTSSQHDVFLCFIPMFHIYGLVFF LGLFCRG TTVLMQRFNFQSMIDAIE YKIN
Sbjct: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKIN 300

Query: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360
           NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES
Sbjct: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360

Query: 361 TGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYL 420
           TGAATWM+TD DAKAHPGSCGMLMPSFY KIVD+ETGEGLPPMK+GELWLKSPTIMK YL
Sbjct: 361 TGAATWMITDKDAKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYL 420

Query: 421 GNKEATEATMDEEGWLKTGDLG-------------------------------------- 480
           GN+EATEAT+DEEGWLKTGDLG                                      
Sbjct: 421 GNREATEATLDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHT 480

Query: 481 ---------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIP 518
                    +EDEAAGQIPVACVVKAP+C LTE+QVIQFVASQVAPY+K+RGVRFIS IP
Sbjct: 481 EILDAAVIPIEDEAAGQIPVACVVKAPSCVLTEQQVIQFVASQVAPYKKVRGVRFISAIP 540

[Close]
BLAST of Lsi04G001120 vs. NCBI nr
Match: XP_011655858.1 (4-coumarate--CoA ligase-like 5 [Cucumis sativus] >KGN52228.1 hypothetical protein Csa_008354 [Cucumis sativus])

HSP 1 Score: 874.0 bits (2257), Expect = 6.4e-250
Identity = 457/553 (82.64%), Postives = 480/553 (86.80%), Query Frame = 0 

Query: 13  VEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSL 72
           VEFQPQ+SPGGYNVNTAVYHSL+ LDEAP  STR DLDTAT+VLSQFPTA+SRVALIDS+
Sbjct: 7   VEFQPQSSPGGYNVNTAVYHSLLHLDEAPVISTRSDLDTATYVLSQFPTAESRVALIDSV 66

Query: 73  TNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTA 132
           T+ RVTYGQLSV IRSLACGLYHALGVRKGDVVFVLS+N VLYPVICLAVLSIGAVITTA
Sbjct: 67  TSLRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVLSIGAVITTA 126

Query: 133 NPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVEELIESCT 192
           NP+NT SEIGKQVRDSGAKLA+SAPEEL KLSPTGIPTILTTRSSYGD LSVEELIESC+
Sbjct: 127 NPMNTESEIGKQVRDSGAKLAVSAPEELQKLSPTGIPTILTTRSSYGDALSVEELIESCS 186

Query: 193 E-SFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDV 252
           E S EPL K EVT SDTAAILYSSGTTGTSKGVVLTHSNLISVIE+LTWCVDSTSSQHDV
Sbjct: 187 ESSSEPLPKAEVTPSDTAAILYSSGTTGTSKGVVLTHSNLISVIEILTWCVDSTSSQHDV 246

Query: 253 FLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGL 312
           FLCFIPMFHIYGLVFF LGLF RG TTVLM RFNFQSMIDAIE YKINNIPAVPPVILGL
Sbjct: 247 FLCFIPMFHIYGLVFFGLGLFRRGITTVLMPRFNFQSMIDAIEKYKINNIPAVPPVILGL 306

Query: 313 VKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDND 372
           VKS GGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWM+TD D
Sbjct: 307 VKSDGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMITDKD 366

Query: 373 AKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDE 432
           AKAHPGSCGMLMPSFY KIVD+ETGEGLPPMK+GELWLKSPTIMK YLGN+EATEAT+DE
Sbjct: 367 AKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYLGNREATEATIDE 426

Query: 433 EGWLKTGDLG-----------------------------------------------MED 492
           EGWLKTGDLG                                               +ED
Sbjct: 427 EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTEILDAAVIPIED 486

Query: 493 EAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDL 518
           EAAGQIPVACVVKAP+C+L+E+QVIQFVASQVAPY+K+RGVRFIS IPRSLAGKILRKDL
Sbjct: 487 EAAGQIPVACVVKAPSCELSEQQVIQFVASQVAPYKKVRGVRFISAIPRSLAGKILRKDL 546

[Close]
BLAST of Lsi04G001120 vs. NCBI nr
Match: XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 872.8 bits (2254), Expect = 1.4e-249
Identity = 457/564 (81.03%), Postives = 484/564 (85.82%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFP 60
           MS EESC APAAVEF+P NSPGGY+VNTAVYHS +QLDEAPK ST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICL 120
           TA+SRVALIDS+T+SRVTYG+LS   RSLA GLYHALGVRKGDVVFVLS+NSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGD 180
           AVLSIGAVITTANP+NTASEIGKQV DS AKLAISAPEELHKLSPTGIPTILTT+ SYGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180

Query: 181 VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
           VLSVEELIESC ESFE + +T++ QSD AAILYSSGTTGTSKGVVLTHSNLISVIELLTW
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINN 300
            VD++SSQ DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQSMIDAIETYK+NN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLG 420
           GAAT M+TD DA+AHPGSCGMLMP F AKIVDIETGEGLPPMKEGE+WLKSPTIMK YL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDEEGWLKTGDLG--------------------------------------- 480
           NKEATEATMD+EGWLKTGDLG                                       
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 --------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPR 518
                    EDEAAGQIPVACVVKAP+CKLTEEQVIQFVASQVA Y+KIRGVRF+S IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

[Close]
BLAST of Lsi04G001120 vs. NCBI nr
Match: XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 866.7 bits (2238), Expect = 1.0e-247
Identity = 456/564 (80.85%), Postives = 483/564 (85.64%), Query Frame = 0 

Query: 1   MSGEESCNAPAAVEFQPQNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFP 60
           MS EESC APAAVEF+P NSPGGY+VNTAVYHSL+Q DEAPK ST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICL 120
           TA+SRVALIDS+T+SRVTYG+LS   RSLA GLYHALGVRKGDVVFVLS+NSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGD 180
           AVLSIGAVITTANP+NTASEIGKQV DS AKLAISAPEELHKLSPTG+PTILTTR SYGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180

Query: 181 VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
           VLSVEELIESC ESFE + KT++ QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINN 300
            VD++SSQ DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQSMIDAIETYK+NN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATWMVTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLG 420
           GAAT M+TD DA+AHPGSCGMLMP F AKIVDIETGEGLPPMKEGE+WLKSPTIMK YL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDEEGWLKTGDLG--------------------------------------- 480
           NKEATEATMD+EGW+KTGDLG                                       
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 --------MEDEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPR 518
                    EDEAAGQIPVACVVKAP+CKLTEEQVIQFVASQVA Y+KIRGVRF+S IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

[Close]
BLAST of Lsi04G001120 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 352.1 bits (902), Expect = 7.8e-97
Identity = 212/542 (39.11%), Postives = 303/542 (55.90%), Query Frame = 0 

Query: 19  NSPGGY-NVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRV 78
           NS  G+ N N+  Y     +   P     P LD  TF+ SQ    + R+A ID+ T   +
Sbjct: 5   NSRSGFCNSNSTFYSKRTPIPLPP----NPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64

Query: 79  TYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINT 138
           T+ +L   + S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65  TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124

Query: 139 ASEIGKQVRDSGAKLAISAPEELHKLSPTG--IPTILTTRSSYGDVLSVEELIESC-TES 198
           ++EI KQ++DS   LA +  + L K+S     +P +L        V  V  L+E    E 
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184

Query: 199 FEPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLC 258
               +K  V Q DTA +LYSSGTTG SKGV+ +H NLI++++ +     S   +   F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244

Query: 259 FIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKS 318
            +PMFHIYGL  FA GL   G+T +++ +F    M+ AI  Y+  ++P VPP+++ +V  
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304

Query: 319 G----GGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDN 378
                   D SS+  V  G APL K+V E F EK+P V++  GYGLTESTG      T  
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364

Query: 379 DAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMD 438
           +++ + G+ G L  S   +IVD  TG+ L P + GELWLK P+IMKGY  N+EAT +T+D
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424

Query: 439 EEGWLKTGDL-----------------------------------------------GME 498
            EGWL+TGDL                                                  
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484

Query: 499 DEAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKD 506
           D+  GQ P+A VV+     L+E+ +++FVA QVAPY++IR V F+S IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537

[Close]
BLAST of Lsi04G001120 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.9e-95
Identity = 204/539 (37.85%), Postives = 296/539 (54.92%), Query Frame = 0 

Query: 30  VYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSL 89
           +Y SL      PK    P+    +F+     +  S++A+ DS T   +T+ QL   +  L
Sbjct: 11  IYRSLRPTLVLPK---DPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 70

Query: 90  ACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSG 149
           A G +H LG+RK DVV + + NS  +P+  LAV +IG V TTANP+ T +E+ KQ++DS 
Sbjct: 71  AHG-FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSN 130

Query: 150 AKLAISAPEELHKLSPTGIPTIL--------TTRSSYGDVLSVEELIESCTESFEPLLKT 209
            K+ IS  +   K+    +P +L            S   +LS + ++E  +E        
Sbjct: 131 PKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFV 190

Query: 210 EVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIPMFHI 269
           E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++T   D     H VFLCF+PMFH+
Sbjct: 191 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHV 250

Query: 270 YGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSG--GGSD 329
           +GL         RG   V M RF  + ++  IE +++ ++  VPPV L L K       D
Sbjct: 251 FGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFD 310

Query: 330 FSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPGSC 389
            SSL+ +GSGAAPLGKD+ E      P V L  GYG+TE+ G  + +      K + GS 
Sbjct: 311 LSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVS-VEDPRLGKRNSGSA 370

Query: 390 GMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKTGD 449
           GML P   A+IV +ETG+  PP ++GE+W++ P +MKGYL N +AT+ T+D++ W+ TGD
Sbjct: 371 GMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGD 430

Query: 450 LG-----------------------------------------------MEDEAAGQIPV 509
           LG                                                 DE AG++P+
Sbjct: 431 LGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPI 490

Query: 510 ACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKILRKDLVSQFKQQ 512
           A VV++P   +TE+ + +F+A QVAPY+++R V FIS++P+S AGKILR++LV Q + +
Sbjct: 491 AFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543

[Close]
BLAST of Lsi04G001120 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 342.0 bits (876), Expect = 8.1e-94
Identity = 210/545 (38.53%), Postives = 305/545 (55.96%), Query Frame = 0 

Query: 18  QNSPGGYNVNTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRV 77
           QN P  ++  T +Y S       P     P+LD  + + S         ALIDSLT   +
Sbjct: 19  QNPPFWFSSKTGIYTSKFPSLHLP---VDPNLDAVSALFSH--KHHGDTALIDSLTGFSI 78

Query: 78  TYGQLSVWIRSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINT 137
           ++ +L + ++S+A G+YH LGVR+GDVV ++  NSV +P+I L+++S+GA++TT NP ++
Sbjct: 79  SHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSS 138

Query: 138 ASEIGKQVRDSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVE--ELIESCTESF 197
             EI KQV +    LA ++ E + KLS  G+  I  + S   D + +E  +      ESF
Sbjct: 139 LGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESF 198

Query: 198 EPLLKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHD----- 257
             + K  + Q D AAI+YSSGTTG SKGV+LTH NLI+ +EL    V   +SQ++     
Sbjct: 199 GFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELF---VRFEASQYEYPGSS 258

Query: 258 -VFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVIL 317
            V+L  +P+ HIYGL  F +GL   G+T V+M+RF+   +++ IE +KI + P VPP+++
Sbjct: 259 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 318

Query: 318 GLVKSGG---GSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWM 377
            L K      G  F SL++V SGAAPL +   E F +  P V+L  GYG+TEST   T  
Sbjct: 319 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT-R 378

Query: 378 VTDNDAKAHPGSCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATE 437
             +++  +   S G+L P+  AK+VD  +G  LPP   GELW++ P +MKGYL N +AT+
Sbjct: 379 GFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQ 438

Query: 438 ATMDEEGWLKTGDLGMED------------------------------------------ 497
            ++ E+ WL+TGD+   D                                          
Sbjct: 439 MSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAV 498

Query: 498 -----EAAGQIPVACVVKAPTCKLTEEQVIQFVASQVAPYRKIRGVRFISVIPRSLAGKI 505
                E  G+IPVA VV+     L+EE VI +VASQVAPYRK+R V  ++ IP+S  GKI
Sbjct: 499 TAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 554

[Close]
BLAST of Lsi04G001120 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 332.0 bits (850), Expect = 8.4e-91
Identity = 204/519 (39.31%), Postives = 299/519 (57.61%), Query Frame = 0 

Query: 49  LDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWIRSLACGLYHALGVRKGDVVFVL 108
           LD  +F+ SQ    + +   +D++T  R+++ +L + +  +A G  +ALGVRKG+VV +L
Sbjct: 49  LDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPELWLGVERVA-GCLYALGVRKGNVVIIL 108

Query: 109 SSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAISAPEELHKL---SP 168
           S NS+L+P++ L+V+S+GA+ITTANPINT+ EI KQ+ DS   LA +  + + KL   S 
Sbjct: 109 SPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSRPVLAFTTCKLVSKLAAASN 168

Query: 169 TGIPTIL-----TTRSSYGD----VLSVEELIESCTESFEPLLKTEVTQSDTAAILYSSG 228
             +P +L         SYGD    V  +E +IE  TE  E  +K  V Q DTAA+LYSSG
Sbjct: 169 FNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIE--TEPSESRVKQRVNQDDTAALLYSSG 228

Query: 229 TTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFALGLFCRGT 288
           TTGTSKGV+L+H NLI++++       +        +C IPM HI+G   FA GL   G 
Sbjct: 229 TTGTSKGVMLSHRNLIALVQ----AYRARFGLEQRTICTIPMCHIFGFGGFATGLIALGW 288

Query: 289 TTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKS----GGGSDFSSLRRVGSGAAP 348
           T V++ +F+   ++ A+ET++ + +  VPP+++ +V          D SSL  V +G AP
Sbjct: 289 TIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAP 348

Query: 349 LGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPGSCGMLMPSFYAKIVD 408
           L ++V E F E +P V++  GYGLTEST  A  M    + K + G+ G+L P+   KIVD
Sbjct: 349 LSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNKEETKRY-GASGLLAPNVEGKIVD 408

Query: 409 IETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKTGDL------------ 468
            +TG  L   + GELW++SPT+MKGY  NKEAT +T+D EGWLKTGDL            
Sbjct: 409 PDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVV 468

Query: 469 -----------------------------------GMEDEAAGQIPVACVVKAPTCKLTE 505
                                               + D  AGQ P+A +V+     L+E
Sbjct: 469 DRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSE 528

[Close]
BLAST of Lsi04G001120 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 328.6 bits (841), Expect = 9.3e-90
Identity = 200/535 (37.38%), Postives = 288/535 (53.83%), Query Frame = 0 

Query: 27  NTAVYHSLIQLDEAPKFSTRPDLDTATFVLSQFPTAKSRVALIDSLTNSRVTYGQLSVWI 86
           N+  Y     L   P  S     D  TF+ SQ    + + A ID+ T   +T+  L   +
Sbjct: 20  NSTFYSKRQPLSLPPNLSR----DVTTFISSQ--PHRGKTAFIDAATGQCLTFSDLWRAV 79

Query: 87  RSLACGLYHALGVRKGDVVFVLSSNSVLYPVICLAVLSIGAVITTANPINTASEIGKQVR 146
             +A  LYH +G+R+GDVV +LS NS+  PV+CL+V+S+GAV TTAN +NT+ EI KQ+ 
Sbjct: 80  DRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIA 139

Query: 147 DSGAKLAISAPEELHKLSPTGIPTILTTRSSYGDVLSVEELIESCTESF--EP---LLKT 206
           DS   L  +  +   KL P  I  +LT    Y ++ S   ++   +E    EP    ++ 
Sbjct: 140 DSNPTLVFTTRQLAPKL-PVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRD 199

Query: 207 EVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVDSTSSQHDVFLCFIPMFHI 266
            V Q DTA +LYSSGTTG SKGV+ +H NL + +      +     + D+F+C +PMFH 
Sbjct: 200 RVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARF---ISDNLKRDDIFICTVPMFHT 259

Query: 267 YGLVFFALGLFCRGTTTVLMQRFNFQSMIDAIETYKINNIPAVPPVILGLVKSG----GG 326
           YGL+ FA+G    G+T V+++RF    M+DA+E ++   +   PPV++ ++         
Sbjct: 260 YGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAK 319

Query: 327 SDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMVTDNDAKAHPG 386
            D SSL+ V  G APL K+V E F EK+P V++  GY LTES G   +  +  +++ + G
Sbjct: 320 YDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRY-G 379

Query: 387 SCGMLMPSFYAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNKEATEATMDEEGWLKT 446
           + G L     A+IVD  TG  +   + GELWLK P+I KGY  N+EAT  T++ EGWLKT
Sbjct: 380 TAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKT 439

Query: 447 GDL-----------------------------------------------GMEDEAAGQI 506
           GDL                                                  D+ AGQ 
Sbjct: 440 GDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQY 499

The following BLAST results are available for this feature:
BLAST of Lsi04G001120 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
M4IQR71.3e-9639.70Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Q84P211.1e-9539.114-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
Q9M0X92.7e-9437.854-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
Q84P241.1e-9238.534-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=... [more]
Q10S724.1e-9039.344-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4... [more]
BLAST of Lsi04G001120 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BFZ33.4e-25783.044-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348942... [more]
A0A0A0KRI43.1e-25082.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1[more]
A0A6J1K3036.9e-25081.034-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ... [more]
A0A6J1H2E64.9e-24880.854-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=... [more]
A0A6J1CEK11.3e-24077.764-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE... [more]
BLAST of Lsi04G001120 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038891768.12.3e-26385.114-coumarate--CoA ligase-like 5 [Benincasa hispida][more]
XP_008446808.17.0e-25783.04PREDICTED: 4-coumarate--CoA ligase-like 5 isoform X1 [Cucumis melo][more]
XP_011655858.16.4e-25082.644-coumarate--CoA ligase-like 5 [Cucumis sativus] >KGN52228.1 hypothetical protei... [more]
XP_022994494.11.4e-24981.034-coumarate--CoA ligase-like 5 [Cucurbita maxima][more]
XP_022957594.11.0e-24780.854-coumarate--CoA ligase 1-like [Cucurbita moschata][more]
BLAST of Lsi04G001120 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G20510.17.8e-9739.11OPC-8:0 CoA ligase1 [more]
AT4G05160.11.9e-9537.85AMP-dependent synthetase and ligase family protein [more]
AT4G19010.18.1e-9438.53AMP-dependent synthetase and ligase family protein [more]
AT1G20480.18.4e-9139.31AMP-dependent synthetase and ligase family protein [more]
AT1G20500.19.3e-9037.38AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 441..498
e-value: 3.1E-5
score: 24.9
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 31..444
e-value: 1.4E-113
score: 381.7
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 63..450
e-value: 4.6E-92
score: 308.8
NoneNo IPR availableGENE3D3.30.300.30coord: 445..492
e-value: 3.0E-6
score: 29.7
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 442..510
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 16..445
NoneNo IPR availablePANTHERPTHR24096:SF376AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATEDcoord: 442..510
NoneNo IPR availablePANTHERPTHR24096:SF376AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATEDcoord: 16..445
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 56..510
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 211..222

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G001120.1Lsi04G001120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016405 CoA-ligase activity