Lsi04G001020 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G001020
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectate lyase
Location	chr04: 1154248 .. 1158072 (-)
RNA-Seq Expression	Lsi04G001020
Synteny	Lsi04G001020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAACTAACAAGTTAAGAATGCAAAATACAAGTAATGAAGGGGAGACTTTGGAAAGAGAAAGAAAAAAAAAAACAAAGAAAAACAGGGGAAGGAGCTTTGTATTATCTTCTAAATCAGCTTCTTCTTATGCAAATCCTACCTCTTCTCAGCCTTTCAAAATTTCTTCCTTTACTGACATGAACCAAAAACCCTAAAACCCCACCAAGAAAGTAACCTTTTTTTTTTAAGCCCCCTTTTATTTGTTTCTTTATAATTATTATTTTAATTGTTGGCTTATTATATAAACACACCCACCATTTTCTCTTTCTCTACAAATTTAGGCTCTCTTAGCAGCCATGTTTTTATTGATGTTTCAGAAACTGGTTTTGTTTGAAAACAGTCTCTTTTTCTTCGATTAATACCGCAGAACAGCACTGTCAGAAACATCCTCAAATTCCTCATTGTACCTTTGAGGATGTTTCCTGTTCTTAGCATTCTCTTGATATGGATGTCTGCTTCAATCTTTGGTTTCGCCTCTGGTTCTTTCAATCTTACATTACCCCATCAGCATCCTGACCCTGATTCTGTTGCTGATGAACTTCAAAGGTAAATTCACATTCTTAAATTTCTTTTTCTGGGTTTTTTTATTTCATTTTTATTGTTGTTCTTGTTGTTTTTCATCTGGGTTTATTTGCAATTGATTGTTTTTAAAAGAAATTGACGAACCCATCTGAAATTTTTTCACTTTTTCGTTTCTTTTCTTGAATTTTCTCATCATTCAAACAGAAATTCATGGTCTGTTTTCTGTTTTCTTATCCTTTTCTTGGAGGATAGTCAATGTGTATCTATTGTTTCGTTTTTTCATATGGGTTTCTAAAAAATTATTTTCTTTGATGAAAATAAAAAAATTCCCTTTGAAAATTCCTCTCATTTTCCCACCACCAAAACAAAAACAGAGCATTCCTCTGTTTCTCTGTTACCTCAAATCCCCTGTTTCTCTGCAGGACAGTAAATGCATCTGTATGGAGAAGACAAATGCTGTCCTTCGAATCAAAGGATCAAACTTTTTCTTGTCTCACCGGAAACCCCATCGATGATTGCTGGCGGTGCGACCCGAATTGGGCGGCAAACCGACAGCGACTGGCCGACTGCGGTATCGGGTTTGGCCGTGACGCCATGGGCGGCAAAGGCGGCAAGATCTACATCGTTACCGACTCGTCGGATTTGGATCCGGCGAACCCTACGCCGGGAACTCTCCGGCACGCTGTGATCCAATTCGAACCACTGTGGATCATCTTCTCCGCCGACATGACTATCCGGCTGAAATACGAACTAATCATAAACAGTTTCAAAACGATCGACGGCCGTGGCGCCAACGTCCACATAACCGGCGGCGGATGCATCACCATTCAATACGTGTCCAATGTCATCATCCACAACGTCCACGTGCACCACTGTAAACCCTCCGGCAATGCCAACATAAGGTCCGTAAAACTAACTTTCAAAAGTTCAAGTTGATATATGGGTTAAGAAAATGTGTGTAATAAACAAAAATATCTTTTTTTTTTTTAATAAAAACAAAAATGGGTGCTAAGTTTTCCAATAAAAGTTTGGCATGTGACGAATATTTTAATTTTTAAAAAATTAAATTGTTTCTAATTTTTTTTACTGTCTACCAAATGCTGGATGCATGAATTTTGACGTTGCATCTAATTATTGCCTTCTTTTATCACATTTATTTTTTATAGTTTTATTTTTATTATTTTTAATACAGAATCACTAAGGGTAGAAATGTTAAAACAAAAAAATTAGTTTGTCCCATAGCATCATATAATTGAAAGAAAAATAGTCTGTGCATCATAGACAACATCTATTTTTATGCATCCCATTAAAGTGTCTAATCATAATTTGTATGTGGCAAAATTTTCATATTTTAATTTTTCTTAGTGTAAGTAATGAGAAGGAATGCATAAATATAGTTGCATCTTGTGAAAACTTGCTTTCGGTGTTTTGAGGCATGAATTTAGAAAATCTATAATTAATGAAGAAAAGCCTAGCTATGAGATAGTAGTGGGCGAAAAAAAGAAAGGTAGCTAGAATGGGAGTATTGTAAAAATCAATTAATAGGTTTATCCAACAACTAAAGTTGGACGAACTCATAAAAGCAAATATGTATTTGTCTCACAAACATGAGTCAATTTGTACCTTTAACTATTCTTAAGATTTGAATACATATGCTTGTTACTATTAAATATTCAAAAACTTAAACTGATAAAGTTATGGACAATACTTAGATCGACCCCAACTCATGTGGGCCACCGGGGGCTATCAGACGGAGATGGGATCTCAATATTTAGCTCAAGAAAGATATGGATCGACCATTGCTCATTGTCATACTGCACAGATGGGCTAATAGATGCAATAATGGGATCAACAGGAATTACAATATCCAACAGCTACTTCTCTCACCATGATGAAGTGATGTTGTTGGGGCACGATGATCGATACGTGCAAGACTCGGGGATGCAAGTCACCATTGCCTTTAACCACTTCGGTGAGGCATTGGTTCAACGCATGCCCCGTTGTAGACATGGCTATATTCACGTTGTCAATAATGACTTCACTGCTTGGGAAATGTATGCCATTGGAGGCAGCGCCCAACCCACTATTAACAGCCAGGGTAATCGTTACACTGCTCCAGTGAATCCTAATGCCAAAGAGGTACATCTCTCTCTCAATTAAAATAATTTATTTTTTTACTCAACCTAACATTTTTATGTGGCTTGTTTAGGTAGACATATATTTATTAAGTAAATGGACGAAGCATTATACCTATAATGTATAATTTAGTTAATTATTAGGTTAAATTATAAGTTTAGTTTCTAAATTTTTAAGATGGTATGTTTATTTAGTCTATGTATTTTAAAAGGTCTTTAATAACTTGTAGAATTTTTAAATTTATTTCATTTTAATTTATATACTTCAAGAAGTTTCTAAAGGAATACGTGTGCTTTCACTTTCGTGTCTAATAACTATACAATTTATTAATTCATTTCACATCAATGTATTTGGCAAGTTGATTTTAAAGTTAGTTCTTAAAATAATGCCCCATAGATTTCTTAAACATAAAAAAAAAAAAAAATGAGAACAAAATAGTCCTAATCATTATAGTATAACAAAATTGATAACGCTCCTAAAATTTATTAGGAAGTTGAGTATTTACTAAAATAATGGAATAACATAAAATTTCAAAAAATTAAATAAAAAAAGAATCAGTGGTGGAGCGGCGGCGGTCATGAGCCGAACTTTTGACTTGAATGTGTGAAGTGGGGCAATGCATGGGGGAAAGGAAGGATTAGATTCCTTATCCCACTTGCTTGGGCGGCGCATGACATTGACGCGCTGCCAATAAATTACCATTTTCTTAATGGCCCCATCTCTATTTTGGCCTTTCTTTTTGTGATTTTTGGCCGGTGTTGGAGGGAGCGGAATTTGAGGAAGAAAAAAAATATTAATAAACAACAAATCAAAACTATCATTTGGAATTTTGTTTTCATATTTTTCGCGTCATATGTCGGTGACGGTGACGGTGACTGACGGCGACCACGATTTGAAATTTGGTGATATTTATAGGTGACGAGACGTTTAGACGCGGCGGAGACGGACTGGGCGGGGTGGAATTGGCGGACAGACGGCGATATTTTGGTGAACGGCGCGTTTTTTGTACCGTCCGGTGCCGGACTTAGCACCCAGTACGGCAAGGCTTCCAGCGTGGAGCCTAAGTCTGTGGCTCTCATCAATCAACTCACTATGAACGCCGGCGTATTGGGCGCCCCCAGGTAA

mRNA sequence

AATTAACTAACAAGTTAAGAATGCAAAATACAAGTAATGAAGGGGAGACTTTGGAAAGAGAAAGAAAAAAAAAAACAAAGAAAAACAGGGGAAGGAGCTTTGTATTATCTTCTAAATCAGCTTCTTCTTATGCAAATCCTACCTCTTCTCAGCCTTTCAAAATTTCTTCCTTTACTGACATGAACCAAAAACCCTAAAACCCCACCAAGAAAGTAACCTTTTTTTTTTAAGCCCCCTTTTATTTGTTTCTTTATAATTATTATTTTAATTGTTGGCTTATTATATAAACACACCCACCATTTTCTCTTTCTCTACAAATTTAGGCTCTCTTAGCAGCCATGTTTTTATTGATGTTTCAGAAACTGGTTTTGTTTGAAAACAGTCTCTTTTTCTTCGATTAATACCGCAGAACAGCACTGTCAGAAACATCCTCAAATTCCTCATTGTACCTTTGAGGATGTTTCCTGTTCTTAGCATTCTCTTGATATGGATGTCTGCTTCAATCTTTGGTTTCGCCTCTGGTTCTTTCAATCTTACATTACCCCATCAGCATCCTGACCCTGATTCTGTTGCTGATGAACTTCAAAGGACAGTAAATGCATCTGTATGGAGAAGACAAATGCTGTCCTTCGAATCAAAGGATCAAACTTTTTCTTGTCTCACCGGAAACCCCATCGATGATTGCTGGCGGTGCGACCCGAATTGGGCGGCAAACCGACAGCGACTGGCCGACTGCGGTATCGGGTTTGGCCGTGACGCCATGGGCGGCAAAGGCGGCAAGATCTACATCGTTACCGACTCGTCGGATTTGGATCCGGCGAACCCTACGCCGGGAACTCTCCGGCACGCTGTGATCCAATTCGAACCACTGTGGATCATCTTCTCCGCCGACATGACTATCCGGCTGAAATACGAACTAATCATAAACAGTTTCAAAACGATCGACGGCCGTGGCGCCAACGTCCACATAACCGGCGGCGGATGCATCACCATTCAATACGTGTCCAATGTCATCATCCACAACGTCCACGTGCACCACTGTAAACCCTCCGGCAATGCCAACATAAGATCGACCCCAACTCATGTGGGCCACCGGGGGCTATCAGACGGAGATGGGATCTCAATATTTAGCTCAAGAAAGATATGGATCGACCATTGCTCATTGTCATACTGCACAGATGGGCTAATAGATGCAATAATGGGATCAACAGGAATTACAATATCCAACAGCTACTTCTCTCACCATGATGAAGTGATGTTGTTGGGGCACGATGATCGATACGTGCAAGACTCGGGGATGCAAGTCACCATTGCCTTTAACCACTTCGGTGAGGCATTGGTTCAACGCATGCCCCGTTGTAGACATGGCTATATTCACGTTGTCAATAATGACTTCACTGCTTGGGAAATGTATGCCATTGGAGGCAGCGCCCAACCCACTATTAACAGCCAGGGTAATCGTTACACTGCTCCAGTGAATCCTAATGCCAAAGAGGTGACGAGACGTTTAGACGCGGCGGAGACGGACTGGGCGGGGTGGAATTGGCGGACAGACGGCGATATTTTGGTGAACGGCGCGTTTTTTGTACCGTCCGGTGCCGGACTTAGCACCCAGTACGGCAAGGCTTCCAGCGTGGAGCCTAAGTCTGTGGCTCTCATCAATCAACTCACTATGAACGCCGGCGTATTGGGCGCCCCCAGGTAA

Coding sequence (CDS)

ATGTTTCCTGTTCTTAGCATTCTCTTGATATGGATGTCTGCTTCAATCTTTGGTTTCGCCTCTGGTTCTTTCAATCTTACATTACCCCATCAGCATCCTGACCCTGATTCTGTTGCTGATGAACTTCAAAGGACAGTAAATGCATCTGTATGGAGAAGACAAATGCTGTCCTTCGAATCAAAGGATCAAACTTTTTCTTGTCTCACCGGAAACCCCATCGATGATTGCTGGCGGTGCGACCCGAATTGGGCGGCAAACCGACAGCGACTGGCCGACTGCGGTATCGGGTTTGGCCGTGACGCCATGGGCGGCAAAGGCGGCAAGATCTACATCGTTACCGACTCGTCGGATTTGGATCCGGCGAACCCTACGCCGGGAACTCTCCGGCACGCTGTGATCCAATTCGAACCACTGTGGATCATCTTCTCCGCCGACATGACTATCCGGCTGAAATACGAACTAATCATAAACAGTTTCAAAACGATCGACGGCCGTGGCGCCAACGTCCACATAACCGGCGGCGGATGCATCACCATTCAATACGTGTCCAATGTCATCATCCACAACGTCCACGTGCACCACTGTAAACCCTCCGGCAATGCCAACATAAGATCGACCCCAACTCATGTGGGCCACCGGGGGCTATCAGACGGAGATGGGATCTCAATATTTAGCTCAAGAAAGATATGGATCGACCATTGCTCATTGTCATACTGCACAGATGGGCTAATAGATGCAATAATGGGATCAACAGGAATTACAATATCCAACAGCTACTTCTCTCACCATGATGAAGTGATGTTGTTGGGGCACGATGATCGATACGTGCAAGACTCGGGGATGCAAGTCACCATTGCCTTTAACCACTTCGGTGAGGCATTGGTTCAACGCATGCCCCGTTGTAGACATGGCTATATTCACGTTGTCAATAATGACTTCACTGCTTGGGAAATGTATGCCATTGGAGGCAGCGCCCAACCCACTATTAACAGCCAGGGTAATCGTTACACTGCTCCAGTGAATCCTAATGCCAAAGAGGTGACGAGACGTTTAGACGCGGCGGAGACGGACTGGGCGGGGTGGAATTGGCGGACAGACGGCGATATTTTGGTGAACGGCGCGTTTTTTGTACCGTCCGGTGCCGGACTTAGCACCCAGTACGGCAAGGCTTCCAGCGTGGAGCCTAAGTCTGTGGCTCTCATCAATCAACTCACTATGAACGCCGGCGTATTGGGCGCCCCCAGGTAA

Protein sequence

MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR

Homology

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BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 595.5 bits (1534), Expect = 4.6e-169
Identity = 272/381 (71.39%), Postives = 313/381 (82.15%), Query Frame = 0


Query: 32  HPDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLA 91
           HPDP+ VA ++Q  VNAS+ RRQ L    +  +  C+TGNPIDDCW+CDPNW  NRQ LA
Sbjct: 35  HPDPELVAYQVQWKVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLA 94

Query: 92  DCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLK 151
           DCGIGFG+ A+GGKGG+ Y VTDSSD D  NP PGTLR+ VIQ EPLWI+F ++M I+LK
Sbjct: 95  DCGIGFGQYALGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLK 154

Query: 152 YELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVG 211
            ELI NS+KT+DGRGANVHI GGGCIT+QYVSN+IIHN+H+HHC  SGN N+RS+PTH G
Sbjct: 155 QELIFNSYKTLDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYG 214

Query: 212 HRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGH 271
            R  SDGDGISIF S+ IWIDHCSLS C DGLIDA+MGSTGITISN++FSHH+EVMLLGH
Sbjct: 215 FRTKSDGDGISIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGH 274

Query: 272 DDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINS 331
            D Y  DSGMQVTIAFNHFGE L+QRMPRCR GYIHVVNNDFT WEMYAIGGS  PTINS
Sbjct: 275 SDHYEPDSGMQVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINS 334

Query: 332 QGNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKAS 391
           QGNRYTAP NP AKEVT+R++  + DW GWNWR++GDILVNGAFFV SG G   +Y KA 
Sbjct: 335 QGNRYTAPTNPFAKEVTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAY 394

Query: 392 SVEPKSVALINQLTMNAGVLG 413
           SVEPKS + I Q+T ++GVLG
Sbjct: 395 SVEPKSASFITQITFHSGVLG 415

[Close]

BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 3.4e-164
Identity = 270/404 (66.83%), Postives = 324/404 (80.20%), Query Frame = 0


Query: 20  ASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQM------LSFESKDQTFSCLTGNPI 79
           A+   NLTLPHQHP PDSVA  + R+VN S+ RRQ+       S  S   + SC TGNPI
Sbjct: 22  ATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPI 81

Query: 80  DDCWRC-DPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAV 139
           DDCWRC D +W+ NRQRLADC IGFG   +GGK GKIY+VTDSSD +P NPTPGTLR+ V
Sbjct: 82  DDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGV 141

Query: 140 IQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHV 199
           IQ EPLWI+FS++M IRLK ELIINS+KT+DGRG+ VHITG GC+T+QYV ++IIHN+H+
Sbjct: 142 IQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHI 201

Query: 200 HHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTG 259
           + CKPS              RG SDGDGISIF S+KIW+DHCS+S+CTDGLIDA+MGST 
Sbjct: 202 YDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTA 261

Query: 260 ITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNND 319
           ITISN+YF+HHDEVMLLGHDD Y  D+GMQVTIAFNHFG+ LVQRMPRCR GYIHVVNND
Sbjct: 262 ITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNND 321

Query: 320 FTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAET-DWAGWNWRTDGDILV 379
           FT W+MYAIGGS  PTINSQGNRY+AP +P+AKEVT+R+D+ +  +W+ WNWRT+GD++ 
Sbjct: 322 FTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLME 381

Query: 380 NGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           NGAFFV SG G+S+ Y KASSV+PK+ +L++QLT NAGV G PR
Sbjct: 382 NGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPR 416

[Close]

BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 540.8 bits (1392), Expect = 1.3e-152
Identity = 245/379 (64.64%), Postives = 299/379 (78.89%), Query Frame = 0


Query: 33  PDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NASV  R+ L +       SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKY 152
           C IGFG++A+GG+ G+IY+VTDS + +P +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGH 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+N+IIH +H+H CK  GNA +RS+P H G 
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 RGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
           R +SDGDG+SIF    +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 392
           GNR+ AP    +KEVT+  DA E++W  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

Query: 393 VEPKSVALINQLTMNAGVL 412
           +  K  +L+  LT  +G L
Sbjct: 388 LGAKPSSLVGPLTSTSGAL 400

[Close]

BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 3.0e-152
Identity = 246/379 (64.91%), Postives = 300/379 (79.16%), Query Frame = 0


Query: 34  DPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADC 93
           DPD VADE+ + V  S    Q  +   K   FSC TGNPIDDCWRCD NW  NR+RLADC
Sbjct: 88  DPDMVADEVAKLVQMS---EQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADC 147

Query: 94  GIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYE 153
           GIGFGR+A+GG+ G+ YIVTD +D D  NP PGTLRHAVIQ EPLWI+F  DM I LK E
Sbjct: 148 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 207

Query: 154 LIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHR 213
           LI+NSFKTID RG+NVHI  G CITIQ+++NVIIH +H+H CKP+GNA +RS+P+H G R
Sbjct: 208 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 267

Query: 214 GLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDD 273
            ++DGD +SIF S  IWIDH SLS+C DGL+DA+MGST IT+SN++F+HH+EVMLLGH D
Sbjct: 268 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 327

Query: 274 RYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQG 333
            Y +D  MQVTIA+NHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA+PTINSQG
Sbjct: 328 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 387

Query: 334 NRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSV 393
           NRY AP++  AKEVT+R++   ++W  WNWR++GD+L+NGAFF PSGAG S  YG+ASS+
Sbjct: 388 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSL 447

Query: 394 EPKSVALINQLTMNAGVLG 413
             K  ++++ +T  AG LG
Sbjct: 448 AAKPSSMVDTITSTAGALG 463

[Close]

BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 538.9 bits (1387), Expect = 5.1e-152
Identity = 247/389 (63.50%), Postives = 298/389 (76.61%), Query Frame = 0


Query: 30  HQHPDPDSVADELQRTVNASV---WRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAAN 89
           H   +PD VADE+      SV     R+ L +      F+C TGNPIDDCWRCDPNW  N
Sbjct: 43  HAVTNPDEVADEVLALTEMSVRNHTERRKLGY------FTCGTGNPIDDCWRCDPNWHKN 102

Query: 90  RQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADM 149
           R+RLADCGIGFGR+A+GG+ G+ Y+VTD  D +P NP PGTLRHAVIQ  PLWI+F  DM
Sbjct: 103 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 162

Query: 150 TIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRST 209
            I+LK ELI+NSFKTIDGRGANVHI  GGCITIQ+V+NVI+H +H+H CKP+GNA +RS+
Sbjct: 163 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 222

Query: 210 PTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEV 269
            TH G R ++DGD ISIF S  +WIDH SLS+C DGL+DA+MGST ITISN++ +HH+EV
Sbjct: 223 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 282

Query: 270 MLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQ 329
           MLLGH D Y++D  MQVTIA+NHFG  L+QRMPRCRHGY HVVNND+T WEMYAIGGSA 
Sbjct: 283 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 342

Query: 330 PTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQ 389
           PTINSQGNRY AP NP AKEVT+R+D   + W GWNWR++GD+L NGA+F  SGA  S  
Sbjct: 343 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 402

Query: 390 YGKASSVEPKSVALINQLTMNAGVLGAPR 416
           Y +ASS+  KS +L+  +T +AG L   R
Sbjct: 403 YARASSLSAKSSSLVGHITSDAGALPCRR 425

[Close]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL
Match: A0A1S3BGT0 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489433 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.7e-239
Identity = 400/416 (96.15%), Postives = 407/416 (97.84%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGF-ASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFE 60
           MFPVL+ L IWM ASI    +S SFNLTLPHQHPDPDSVADELQRTVNAS+WRRQMLS E
Sbjct: 1   MFPVLTTLFIWMFASISCVSSSSSFNLTLPHQHPDPDSVADELQRTVNASIWRRQMLSIE 60

Query: 61  SKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD 120
           SKDQTFSCLTGNPIDDCWRCDPNW+ANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD
Sbjct: 61  SKDQTFSCLTGNPIDDCWRCDPNWSANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD 120

Query: 121 PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI 180
           PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI
Sbjct: 121 PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI 180

Query: 181 QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC 240
           QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC
Sbjct: 181 QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC 240

Query: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300
           TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP
Sbjct: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300

Query: 301 RCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWA 360
           RCRHGYIHVVNNDFTAW+MYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+WA
Sbjct: 301 RCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWA 360

Query: 361 GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416

[Close]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL
Match: A0A0A0KRG9 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G622520 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.4e-237
Identity = 400/417 (95.92%), Postives = 407/417 (97.60%), Query Frame = 0


Query: 1   MFP-VLSILLIWM-SASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSF 60
           MFP VL+ L IWM ++SI   +S SFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLS 
Sbjct: 1   MFPRVLTTLFIWMVASSISCVSSSSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSI 60

Query: 61  ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL 120
           ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL
Sbjct: 61  ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL 120

Query: 121 DPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT 180
           DP NPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT
Sbjct: 121 DPTNPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT 180

Query: 181 IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY 240
           IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY
Sbjct: 181 IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY 240

Query: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300
           CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM
Sbjct: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300

Query: 301 PRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDW 360
           PRCRHGYIHVVNNDFTAW+MYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+W
Sbjct: 301 PRCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEW 360

Query: 361 AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 417

[Close]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL
Match: A0A6J1CBN1 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111010036 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.5e-236
Identity = 390/415 (93.98%), Postives = 404/415 (97.35%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFES 60
           MFPVLSILLIWMS S+F  AS SFNLTLPHQHPDPDSVADELQR VNAS+ RRQMLS ES
Sbjct: 1   MFPVLSILLIWMSGSVFNVASASFNLTLPHQHPDPDSVADELQRRVNASISRRQMLSLES 60

Query: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120
           KD+ FSCL+GNPIDDCWRCDPNWAANRQRLADCGIGFGRDA+GGKGGKIYIV+DSSD DP
Sbjct: 61  KDEAFSCLSGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAVGGKGGKIYIVSDSSDSDP 120

Query: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELI+NSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIVNSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240
           YVSN+IIHN+HVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAG 360
           CRHGYIHVVNNDFTAWEMYAIGGSA+PTINSQGNRYTAP++PNAKEVTRRLDAAETDW+ 
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYTAPLDPNAKEVTRRLDAAETDWSA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           WNWRTDGDILVNGAFFVPSGAG+STQYGKASSVEPKSVALINQLTMNAGVL APR
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGVSTQYGKASSVEPKSVALINQLTMNAGVLAAPR 415

[Close]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL
Match: A0A5D3CBM7 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002370 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 6.5e-235
Identity = 388/396 (97.98%), Postives = 394/396 (99.49%), Query Frame = 0


Query: 20  ASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRC 79
           +S SFNLTLPHQHPDPDSVADELQRTVNAS+WRRQMLS ESKDQTFSCLTGNPIDDCWRC
Sbjct: 10  SSSSFNLTLPHQHPDPDSVADELQRTVNASIWRRQMLSIESKDQTFSCLTGNPIDDCWRC 69

Query: 80  DPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLW 139
           DPNW+ANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLW
Sbjct: 70  DPNWSANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLW 129

Query: 140 IIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSG 199
           IIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSG
Sbjct: 130 IIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSG 189

Query: 200 NANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSY 259
           NANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSY
Sbjct: 190 NANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSY 249

Query: 260 FSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMY 319
           FSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAW+MY
Sbjct: 250 FSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWQMY 309

Query: 320 AIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPS 379
           AIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+WAGWNWRTDGDILVNGAFFVPS
Sbjct: 310 AIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWAGWNWRTDGDILVNGAFFVPS 369

Query: 380 GAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           GAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 370 GAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 405

[Close]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL
Match: A0A6J1G261 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111449983 PE=3 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 1.2e-233
Identity = 389/415 (93.73%), Postives = 398/415 (95.90%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFES 60
           MFPVLSILLIWMS   F  AS SFNLTLPHQHPDPDSVADELQRTVNAS+ RR+ LS ES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120
           KDQ FSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSD +P
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
            NPTPGTLRHAVIQFEPLWIIFSADMTI+LKYELIIN+FKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINNFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240
           YVSN+IIHN+HVHHC PSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAG 360
           CRHGYIHVVNNDFTAWEMYAIGGSA+PTINSQGNRY APVNPNAKEVTRRLDAAET+W  
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASS EPKSVALINQLTMNAGVLGAPR
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPR 415

[Close]

BLAST of Lsi04G001020 vs. NCBI nr
Match: XP_038893102.1 (probable pectate lyase 12 isoform X2 [Benincasa hispida])

HSP 1 Score: 858.6 bits (2217), Expect = 2.2e-245
Identity = 408/415 (98.31%), Postives = 413/415 (99.52%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFES 60
           MFPVL+IL +WMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRR+MLS ES
Sbjct: 1   MFPVLAILFVWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRKMLSIES 60

Query: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120
           KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP
Sbjct: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120

Query: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240
           YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT
Sbjct: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAG 360
           CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+WAG
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWAG 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 415

[Close]

BLAST of Lsi04G001020 vs. NCBI nr
Match: XP_038893100.1 (probable pectate lyase 18 isoform X1 [Benincasa hispida] >XP_038893101.1 probable pectate lyase 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 858.6 bits (2217), Expect = 2.2e-245
Identity = 408/415 (98.31%), Postives = 413/415 (99.52%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFES 60
           MFPVL+IL +WMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRR+MLS ES
Sbjct: 1   MFPVLAILFVWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRKMLSIES 60

Query: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120
           KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP
Sbjct: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120

Query: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240
           YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT
Sbjct: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAG 360
           CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+WAG
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWAG 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 415

[Close]

BLAST of Lsi04G001020 vs. NCBI nr
Match: XP_008446817.1 (PREDICTED: probable pectate lyase 18 [Cucumis melo])

HSP 1 Score: 836.3 bits (2159), Expect = 1.2e-238
Identity = 400/416 (96.15%), Postives = 407/416 (97.84%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGF-ASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFE 60
           MFPVL+ L IWM ASI    +S SFNLTLPHQHPDPDSVADELQRTVNAS+WRRQMLS E
Sbjct: 1   MFPVLTTLFIWMFASISCVSSSSSFNLTLPHQHPDPDSVADELQRTVNASIWRRQMLSIE 60

Query: 61  SKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD 120
           SKDQTFSCLTGNPIDDCWRCDPNW+ANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD
Sbjct: 61  SKDQTFSCLTGNPIDDCWRCDPNWSANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD 120

Query: 121 PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI 180
           PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI
Sbjct: 121 PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI 180

Query: 181 QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC 240
           QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC
Sbjct: 181 QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC 240

Query: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300
           TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP
Sbjct: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300

Query: 301 RCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWA 360
           RCRHGYIHVVNNDFTAW+MYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+WA
Sbjct: 301 RCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWA 360

Query: 361 GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416

[Close]

BLAST of Lsi04G001020 vs. NCBI nr
Match: XP_011655861.1 (probable pectate lyase 18 [Cucumis sativus] >KGN52235.1 hypothetical protein Csa_008983 [Cucumis sativus])

HSP 1 Score: 831.6 bits (2147), Expect = 2.9e-237
Identity = 400/417 (95.92%), Postives = 407/417 (97.60%), Query Frame = 0


Query: 1   MFP-VLSILLIWM-SASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSF 60
           MFP VL+ L IWM ++SI   +S SFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLS 
Sbjct: 1   MFPRVLTTLFIWMVASSISCVSSSSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSI 60

Query: 61  ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL 120
           ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL
Sbjct: 61  ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL 120

Query: 121 DPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT 180
           DP NPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT
Sbjct: 121 DPTNPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT 180

Query: 181 IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY 240
           IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY
Sbjct: 181 IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY 240

Query: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300
           CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM
Sbjct: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300

Query: 301 PRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDW 360
           PRCRHGYIHVVNNDFTAW+MYAIGGSAQPTINSQGNRYTAPV+PNAKEVTRRLDAAET+W
Sbjct: 301 PRCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEW 360

Query: 361 AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR
Sbjct: 361 AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 417

[Close]

BLAST of Lsi04G001020 vs. NCBI nr
Match: XP_022138994.1 (probable pectate lyase 18 [Momordica charantia])

HSP 1 Score: 826.6 bits (2134), Expect = 9.4e-236
Identity = 390/415 (93.98%), Postives = 404/415 (97.35%), Query Frame = 0


Query: 1   MFPVLSILLIWMSASIFGFASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSFES 60
           MFPVLSILLIWMS S+F  AS SFNLTLPHQHPDPDSVADELQR VNAS+ RRQMLS ES
Sbjct: 1   MFPVLSILLIWMSGSVFNVASASFNLTLPHQHPDPDSVADELQRRVNASISRRQMLSLES 60

Query: 61  KDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDP 120
           KD+ FSCL+GNPIDDCWRCDPNWAANRQRLADCGIGFGRDA+GGKGGKIYIV+DSSD DP
Sbjct: 61  KDEAFSCLSGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAVGGKGGKIYIVSDSSDSDP 120

Query: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELI+NSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIVNSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240
           YVSN+IIHN+HVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAETDWAG 360
           CRHGYIHVVNNDFTAWEMYAIGGSA+PTINSQGNRYTAP++PNAKEVTRRLDAAETDW+ 
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYTAPLDPNAKEVTRRLDAAETDWSA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           WNWRTDGDILVNGAFFVPSGAG+STQYGKASSVEPKSVALINQLTMNAGVL APR
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGVSTQYGKASSVEPKSVALINQLTMNAGVLAAPR 415

[Close]

BLAST of Lsi04G001020 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 638.6 bits (1646), Expect = 3.4e-183
Identity = 297/422 (70.38%), Postives = 349/422 (82.70%), Query Frame = 0


Query: 7   ILLIW----MSASIFGFASGS----FNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSF 66
           I +IW    +S S  G AS +    FNL+LPHQHP P+ V   +QR +N S+ RRQ+L++
Sbjct: 17  ICIIWFCLLVSLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQRKLNDSLSRRQLLTY 76

Query: 67  ESKDQTF------SCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIV 126
           +  D T       SC+TGNPIDDCWRCDPNW+ANRQRLADC IGFG+  +GGKGG+ Y+V
Sbjct: 77  QQDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADCSIGFGQGTLGGKGGQFYLV 136

Query: 127 TDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHIT 186
           TDSSD D ANP PGTLRHAVIQ EPLWIIFS+DM I+LK+ELII S+KTIDGRG N+ IT
Sbjct: 137 TDSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHELIIGSYKTIDGRGTNIQIT 196

Query: 187 GGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWID 246
           G GC+TIQ VS+VIIHNVH+HHCKPSGN  + S+PTHVG RG+SDGDGIS+ +S  IW+D
Sbjct: 197 GHGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFRGVSDGDGISVSASHHIWVD 256

Query: 247 HCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGE 306
           HCSL YC DGLID I+ ST +TISN+YFSHHDEVMLLGHDDRY  D GMQVTIAFNHFGE
Sbjct: 257 HCSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDDRYTADKGMQVTIAFNHFGE 316

Query: 307 ALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLD 366
            LVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSA PTINSQGNRYTAP++PNAKEVT+R+D
Sbjct: 317 GLVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQGNRYTAPIDPNAKEVTKRVD 376

Query: 367 AAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLG 415
           + E  W+GWNWRT+GD++VNGAFFVPSG G+S  Y +A+SV+PK+ A+I+QLT+NAGV G
Sbjct: 377 SNEKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFG 436

[Close]

BLAST of Lsi04G001020 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 595.5 bits (1534), Expect = 3.3e-170
Identity = 272/381 (71.39%), Postives = 313/381 (82.15%), Query Frame = 0


Query: 32  HPDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLA 91
           HPDP+ VA ++Q  VNAS+ RRQ L    +  +  C+TGNPIDDCW+CDPNW  NRQ LA
Sbjct: 35  HPDPELVAYQVQWKVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLA 94

Query: 92  DCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLK 151
           DCGIGFG+ A+GGKGG+ Y VTDSSD D  NP PGTLR+ VIQ EPLWI+F ++M I+LK
Sbjct: 95  DCGIGFGQYALGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLK 154

Query: 152 YELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVG 211
            ELI NS+KT+DGRGANVHI GGGCIT+QYVSN+IIHN+H+HHC  SGN N+RS+PTH G
Sbjct: 155 QELIFNSYKTLDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYG 214

Query: 212 HRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGH 271
            R  SDGDGISIF S+ IWIDHCSLS C DGLIDA+MGSTGITISN++FSHH+EVMLLGH
Sbjct: 215 FRTKSDGDGISIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGH 274

Query: 272 DDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINS 331
            D Y  DSGMQVTIAFNHFGE L+QRMPRCR GYIHVVNNDFT WEMYAIGGS  PTINS
Sbjct: 275 SDHYEPDSGMQVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINS 334

Query: 332 QGNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKAS 391
           QGNRYTAP NP AKEVT+R++  + DW GWNWR++GDILVNGAFFV SG G   +Y KA 
Sbjct: 335 QGNRYTAPTNPFAKEVTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAY 394

Query: 392 SVEPKSVALINQLTMNAGVLG 413
           SVEPKS + I Q+T ++GVLG
Sbjct: 395 SVEPKSASFITQITFHSGVLG 415

[Close]

BLAST of Lsi04G001020 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 579.3 bits (1492), Expect = 2.4e-165
Identity = 270/404 (66.83%), Postives = 324/404 (80.20%), Query Frame = 0


Query: 20  ASGSFNLTLPHQHPDPDSVADELQRTVNASVWRRQM------LSFESKDQTFSCLTGNPI 79
           A+   NLTLPHQHP PDSVA  + R+VN S+ RRQ+       S  S   + SC TGNPI
Sbjct: 22  ATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPI 81

Query: 80  DDCWRC-DPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAV 139
           DDCWRC D +W+ NRQRLADC IGFG   +GGK GKIY+VTDSSD +P NPTPGTLR+ V
Sbjct: 82  DDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGV 141

Query: 140 IQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHV 199
           IQ EPLWI+FS++M IRLK ELIINS+KT+DGRG+ VHITG GC+T+QYV ++IIHN+H+
Sbjct: 142 IQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHI 201

Query: 200 HHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTG 259
           + CKPS              RG SDGDGISIF S+KIW+DHCS+S+CTDGLIDA+MGST 
Sbjct: 202 YDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTA 261

Query: 260 ITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNND 319
           ITISN+YF+HHDEVMLLGHDD Y  D+GMQVTIAFNHFG+ LVQRMPRCR GYIHVVNND
Sbjct: 262 ITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNND 321

Query: 320 FTAWEMYAIGGSAQPTINSQGNRYTAPVNPNAKEVTRRLDAAET-DWAGWNWRTDGDILV 379
           FT W+MYAIGGS  PTINSQGNRY+AP +P+AKEVT+R+D+ +  +W+ WNWRT+GD++ 
Sbjct: 322 FTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLME 381

Query: 380 NGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPR 416
           NGAFFV SG G+S+ Y KASSV+PK+ +L++QLT NAGV G PR
Sbjct: 382 NGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPR 416

[Close]

BLAST of Lsi04G001020 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.8 bits (1392), Expect = 9.6e-154
Identity = 245/379 (64.64%), Postives = 299/379 (78.89%), Query Frame = 0


Query: 33  PDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NASV  R+ L +       SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKY 152
           C IGFG++A+GG+ G+IY+VTDS + +P +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGH 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+N+IIH +H+H CK  GNA +RS+P H G 
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 RGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
           R +SDGDG+SIF    +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 392
           GNR+ AP    +KEVT+  DA E++W  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

Query: 393 VEPKSVALINQLTMNAGVL 412
           +  K  +L+  LT  +G L
Sbjct: 388 LGAKPSSLVGPLTSTSGAL 400

[Close]

BLAST of Lsi04G001020 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.8 bits (1392), Expect = 9.6e-154
Identity = 245/379 (64.64%), Postives = 299/379 (78.89%), Query Frame = 0


Query: 33  PDPDSVADELQRTVNASVWRRQMLSFESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NASV  R+ L +       SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDLDPANPTPGTLRHAVIQFEPLWIIFSADMTIRLKY 152
           C IGFG++A+GG+ G+IY+VTDS + +P +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGH 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+N+IIH +H+H CK  GNA +RS+P H G 
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 RGLSDGDGISIFSSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
           R +SDGDG+SIF    +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSAQPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYTAPVNPNAKEVTRRLDAAETDWAGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 392
           GNR+ AP    +KEVT+  DA E++W  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

Query: 393 VEPKSVALINQLTMNAGVL 412
           +  K  +L+  LT  +G L
Sbjct: 388 LGAKPSSLVGPLTSTSGAL 400

The following BLAST results are available for this feature:

BLAST of Lsi04G001020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCP2	4.6e-169	71.39	Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2	[more]
Q93Z04	3.4e-164	66.83	Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1	[more]
Q9C5M8	1.3e-152	64.64	Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2	[more]
Q944R1	3.0e-152	64.91	Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1	[more]
Q940Q1	5.1e-152	63.50	Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2	[more]

BLAST of Lsi04G001020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BGT0	5.7e-239	96.15	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489433 PE=3 SV=1	[more]
A0A0A0KRG9	1.4e-237	95.92	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G622520 PE=3 SV=1	[more]
A0A6J1CBN1	4.5e-236	93.98	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111010036 PE=3 SV=1	[more]
A0A5D3CBM7	6.5e-235	97.98	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002370...	[more]
A0A6J1G261	1.2e-233	93.73	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111449983 PE=3 SV=1	[more]

BLAST of Lsi04G001020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893102.1	2.2e-245	98.31	probable pectate lyase 12 isoform X2 [Benincasa hispida]	[more]
XP_038893100.1	2.2e-245	98.31	probable pectate lyase 18 isoform X1 [Benincasa hispida] >XP_038893101.1 probabl...	[more]
XP_008446817.1	1.2e-238	96.15	PREDICTED: probable pectate lyase 18 [Cucumis melo]	[more]
XP_011655861.1	2.9e-237	95.92	probable pectate lyase 18 [Cucumis sativus] >KGN52235.1 hypothetical protein Csa...	[more]
XP_022138994.1	9.4e-236	93.98	probable pectate lyase 18 [Momordica charantia]	[more]

BLAST of Lsi04G001020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04310.1	3.4e-183	70.38	Pectin lyase-like superfamily protein	[more]
AT3G53190.1	3.3e-170	71.39	Pectin lyase-like superfamily protein	[more]
AT3G54920.1	2.4e-165	66.83	Pectin lyase-like superfamily protein	[more]
AT4G24780.1	9.6e-154	64.64	Pectin lyase-like superfamily protein	[more]
AT4G24780.2	9.6e-154	64.64	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 93..110 score: 57.67 coord: 153..169 score: 73.95 coord: 217..238 score: 63.2 coord: 117..142 score: 64.47 coord: 297..316 score: 68.57 coord: 319..338 score: 73.57 coord: 361..385 score: 51.24 coord: 390..413 score: 29.96
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 145..342 e-value: 2.0E-95 score: 333.0
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 154..334 e-value: 1.7E-23 score: 83.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 70..412 e-value: 1.1E-116 score: 391.5
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 10..413
None	No IPR available	PANTHER	PTHR31683:SF122	PECTATE LYASE	coord: 10..413
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 60..388

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G001020.1	Lsi04G001020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity