Lsi04G000820 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G000820 vs. ExPASy Swiss-Prot
Match: Q9SS47 (Pumilio homolog 4 OS=Arabidopsis thaliana OX=3702 GN=APUM4 PE=1 SV=2)

HSP 1 Score: 804.7 bits (2077), Expect = 1.2e-231
Identity = 496/1001 (49.55%), Postives = 632/1001 (63.14%), Query Frame = 0

Query: 23  NFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVGSLFTSSYYS 82
           + ED  + ELE L++++ N    G  R+ D++++RSGSAPPTVEG L A+ + + ++  S
Sbjct: 23  DLEDRFQRELESLLQQHRNQQSFGRERERDIDVHRSGSAPPTVEGLLRAMDNQYLNNNNS 82

Query: 83  EFN-----TKSGSNDGV--LSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDWRVAQR 142
           +       +   +++GV  LS+DE+R HP+YLSYYYSN++ NPRLPPPL+S+EDWRVAQR
Sbjct: 83  DHRDVGNISSITTSNGVELLSDDELRWHPEYLSYYYSNEHSNPRLPPPLLSREDWRVAQR 142

Query: 143 FQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANGKNLPR 202
           F  S S     G+W  KK V+ + SSSLFS+QPG  V++ +   +++ME  +A  +   +
Sbjct: 143 FHNSESVFDPVGEWR-KKTVEVDNSSSLFSVQPGVPVEQAE---NDLMELRNAVAQGRSQ 202

Query: 203 KALSEWHDRGREGFVGSCG-NGLGARRKSFADIVQEGLGESASLSGQLSRPASHNSFGDV 262
           K   +  D+GRE  +G  G +GLG RRKSFADI+QEGL   A+L  QLSRPAS N+F D+
Sbjct: 203 KV--QRLDQGREDLIGLSGYSGLGPRRKSFADILQEGLERDAALGSQLSRPASCNTFRDM 262

Query: 263 -DNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRSTTP 322
            D   +++    G  + L   + LH++         S N  +  +  +P    + R+ TP
Sbjct: 263 KDAAVLSNFSAGGFDSPLAFHDSLHSTAK------NSPNTMLGSTMSSP----VPRNRTP 322

Query: 323 EPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPD-ISGLHLSSIRPED 382
           +  LVGRS++SGLPP+G+RV PVEKKN   + +QN  S    ++ D +S L++S +  + 
Sbjct: 323 DSHLVGRSTASGLPPIGTRVGPVEKKNTFGTAIQNCESYTAADVADTLSRLNMSEM-SQV 382

Query: 383 GVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLARKSGIVVNPR 442
             N +QS+LQ++L  QSD +  + NG H + L + +  +  +   + +    SG   +  
Sbjct: 383 KENHMQSQLQVELENQSDVMRYIPNG-HKKALRQQNTAETKDHLFSANYGGMSGYGASLG 442

Query: 443 ASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVDLTGYTSGDFS 502
           AST+ SH  VN PKRTSS  SLYS  + S   S      +++N N+   D +  T+G + 
Sbjct: 443 ASTVGSHGQVNIPKRTSSSASLYSTSDHSRLGSVGLSDVNIRNGNINGTDFS--TAGGYM 502

Query: 503 INMNHSSAMNSYGA-SDHIKLPSGTSDR------ANHAGSSL-----QPH---------- 562
                +S    Y A   H+   +G  DR       N   S L      PH          
Sbjct: 503 AKNKLNSLAEHYSAEGSHL---TGDGDRQSLNRLINQVASELHSPVMDPHYSQYLHTASS 562

Query: 563 -----------NYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTP 622
                      N +G S GD     + YL  LLAQ +Q       GN +  N R + +  
Sbjct: 563 TAAPIDHSLIRNNFGTSNGD---TANEYLAMLLAQNRQQL-----GNLNAANSRFFESPS 622

Query: 623 YGSGIPYLADQALDSGLSSVGHGGTVLQNERILRFNSMMRSSIGA-QG------------ 682
           Y  G  YL +      L S        QN R+ +  SMM+   G  QG            
Sbjct: 623 YDLGNMYLGNH-----LPSPSKNSRNFQNMRMSQSASMMKVPFGGLQGSSHVDIGSTAEA 682

Query: 683 ---------------------------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHAR 742
                                      MDQYGSRFIQQKLETA  EEK  IFPEI+P+ R
Sbjct: 683 SLLEGFKNNKTRSLELSEIVGHVIEFSMDQYGSRFIQQKLETATDEEKNAIFPEILPYGR 742

Query: 743 TLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQ 802
           TLMTDVFGNYVIQKFFEHGT  QRKELA+Q++GHVL LSLQMYGCRVIQKALEVV+ +QQ
Sbjct: 743 TLMTDVFGNYVIQKFFEHGTTKQRKELAEQVTGHVLALSLQMYGCRVIQKALEVVELEQQ 802

Query: 803 TQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVI 862
            +MV ELDGSVMKCV DQNGNHVIQKCIE +PQD IQFIIS+FYG+VLALSTHPYGCRVI
Sbjct: 803 ARMVKELDGSVMKCVHDQNGNHVIQKCIERLPQDWIQFIISSFYGKVLALSTHPYGCRVI 862

Query: 863 QRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQI 922
           QRVLEH DD  TQ+IIM+EIM SVC L+QDQYGNYVIQH+++ GKPHERS II+KLAGQI
Sbjct: 863 QRVLEHIDDIETQRIIMEEIMDSVCTLAQDQYGNYVIQHIIQHGKPHERSEIINKLAGQI 922

Query: 923 VKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLE 939
           VKMSQQKFASNVVEKCLTFG PEERQ+L+NE+LG TDENEPLQAMMKDPFGNYVVQKVLE
Sbjct: 923 VKMSQQKFASNVVEKCLTFGGPEERQVLVNEMLGYTDENEPLQAMMKDPFGNYVVQKVLE 982

BLAST of Lsi04G000820 vs. ExPASy Swiss-Prot
Match: Q9ZW07 (Pumilio homolog 1 OS=Arabidopsis thaliana OX=3702 GN=APUM1 PE=1 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.0e-182
Identity = 421/1016 (41.44%), Postives = 569/1016 (56.00%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN++RSGSAPPTV+GS++A G
Sbjct: 14  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLFRSGSAPPTVDGSVSAAG 73

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 74  GLFSGGGGAPFLEFGGVNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 133

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N + SLFSM PG   ++T               
Sbjct: 134 RVAQRLKGSNNVLGGVGD---RRKV--NDNRSLFSMPPGFEGEKTG-------------- 193

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
                 + SEW      G +G  G GLG ++KSFADI Q  +G    ++ Q SRPAS N+
Sbjct: 194 -----ASASEW---DANGLIGLPGLGLGGKQKSFADIFQADMGHGHPVAQQPSRPASRNT 253

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  +S+     S+LSR+
Sbjct: 254 FD--ENVDSNNNLSPSASQGIG--------------------APSPYSYAAVLGSSLSRN 313

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS R+   +K+N +     NG ++G  E  D    +SGL+L
Sbjct: 314 GTPDPQAIARVPSPCLTPIGSGRMSSNDKRNTSNQSPFNGVTSGLNESSDLVNALSGLNL 373

Query: 374 S-SIRPEDGVNGVQSRLQLDLGEQSDFLINMSNG---IHTRTLPELSDKK---------- 433
           S S+  +D     +S+ + D+ +  +++  +  G   ++    P  SD+           
Sbjct: 374 SCSVGLDD-----RSQAEQDVEKVRNYMFGLQGGHNEVNQHEFPNKSDQAHKATGSLRNS 433

Query: 434 ---------------LSNPSDNIDLAR--KSGIVVNPRASTMSSHDHVNFPKRTSSFTSL 493
                          L+NP   +D      +   +NP  ++M ++   N     ++F  +
Sbjct: 434 QLRGPHGSAYNGGVGLANPYQQLDSPNYCLNNYALNPAVASMMANQLGN-----NNFAPM 493

Query: 494 YSKPNSSGFVSKDGPTRHLQN---ANLQNVDLTGYTSGDFSINMNHSSAMNSYGASDHIK 553
           Y   ++ GF   D  +RH      ++ QN+       G FS  M    A    G   H+ 
Sbjct: 494 YDNVSALGFSGMD--SRHHGRGFVSSGQNLS-ESRNLGRFSNRMMGGGA----GLQSHMV 553

Query: 554 LP--SGTSDRANHAGSSLQPHNYY-GISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSS 613
            P  +  +D  +         N+  G S  D+  L+ AYL A    QK  Y +    + S
Sbjct: 554 DPMYNQYADSLDLLNDPSMDRNFMGGSSYMDMLELQRAYLGA----QKSQYGVPYK-SGS 613

Query: 614 VYNHRLYTNTPYGSGIPY----LADQALDSGLSS-------------------------- 673
             +H  Y +  +GS + Y    LA   + + L S                          
Sbjct: 614 PNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRDEVNMRFPSATRNYSGGLM 673

Query: 674 ----------VGHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETANV 733
                      G G ++L+  +  +      S I         DQYGSRFIQQKLETA  
Sbjct: 674 GSWHMDASFDEGFGSSMLEEFKSNKTRGFELSEIAGHVVEFSSDQYGSRFIQQKLETATT 733

Query: 734 EEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGC 793
           +EK  ++ EI+P A  LMTDVFGNYVIQKFFEHG   QR+ELA++L  HVLPLSLQMYGC
Sbjct: 734 DEKNMVYEEIMPQALVLMTDVFGNYVIQKFFEHGLPPQRRELAEKLFDHVLPLSLQMYGC 793

Query: 794 RVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYG 853
           RVIQKA+EVVD DQ+ +MV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F+G
Sbjct: 794 RVIQKAIEVVDLDQKIKMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFG 853

Query: 854 QVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGK 913
            V+ LSTHPYGCRVIQRVLEHC D +TQ  +M+EI+ +V +L+QDQYGNYV+QHVLE GK
Sbjct: 854 HVVTLSTHPYGCRVIQRVLEHCHDPDTQSKVMEEILSTVSMLAQDQYGNYVVQHVLEHGK 913

Query: 914 PHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAM 939
           P ER+ II +LAG+IV+MSQQKFASNVVEKCLTFG PEER+LL+NE+LG+TDENEPLQAM
Sbjct: 914 PDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFGGPEERELLVNEMLGTTDENEPLQAM 953

BLAST of Lsi04G000820 vs. ExPASy Swiss-Prot
Match: Q9ZW06 (Pumilio homolog 2 OS=Arabidopsis thaliana OX=3702 GN=APUM2 PE=1 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 6.6e-182
Identity = 415/1005 (41.29%), Postives = 560/1005 (55.72%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN+YRSGSAPPTV+GS++A G
Sbjct: 14  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLYRSGSAPPTVDGSVSAAG 73

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 74  GLFSGGGGAPFLEFGGGNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 133

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N S SLFSM PG      D+   +  E    + 
Sbjct: 134 RVAQRLKGSSNVLGGVGD---RRKV--NDSQSLFSMPPG-----FDQRKQHEFEVEKTSA 193

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
            +      SEW      G +G  G G+G ++KSFADI Q  +G    ++ Q SRPAS N+
Sbjct: 194 SS------SEW---DANGLIGLPGLGIGGKQKSFADIFQADMGHGHPVTKQPSRPASRNT 253

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  +S+     S+LSR+
Sbjct: 254 FD--ENVDSKNNLSPSASQGIG--------------------APSPYSYAAVLGSSLSRN 313

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS RV   +K+N   +  Q+ ++ G  E  D    +SG++L
Sbjct: 314 GTPDPQAIARVPSPCLTPIGSGRVSSNDKRN---TSNQSPFNGGLNESSDLVNALSGMNL 373

Query: 374 SSIRPEDGVNGVQSRLQL--DLGEQSDFLINMSNG---IHTRTLPELSD--KKLSNPSDN 433
           S      G  G+  R Q   D+ +  +++  +  G   ++    P  SD  +K +    N
Sbjct: 374 S------GSGGLDERGQAEQDVEKVRNYMFGLQGGHNEVNQHGFPNKSDQAQKATGLLRN 433

Query: 434 IDLARKSGIVVNPRASTMSSHDHVNFPK--------------------RTSSFTSLYSKP 493
             L    G   N      + + H++ P                      T++++ +Y   
Sbjct: 434 SQLRGAQGSTYNDGGGVATQYQHLDSPNYCLNNYGLNPAVASMMANQLGTNNYSPVYENA 493

Query: 494 NSSGFVSKDGPTRHLQNANL----QNVDLTGYTSGDFSINMNHSSAMNSYGASDHIKLPS 553
           +++  +   G    L         QN+  +       +  M   + + S+ A       +
Sbjct: 494 SAASAMGFSGMDSRLHGGGYVSSGQNLSESRNLGRFSNRMMGGGTGLQSHMADPMYHQYA 553

Query: 554 GTSDRANHAGSSLQPHNYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSSVYN--- 613
            + D  N     +   N+ G S  ++  L+ AYL A  +Q    Y+     + + Y    
Sbjct: 554 DSLDLLNDPSMDV---NFMGNSYMNMLELQRAYLGAQKSQYGVPYKSGSPNSHTDYGSPT 613

Query: 614 ----------HRLYTNT----------------------PYGSGIPYLADQALDSGLSSV 673
                     H L  N+                       Y  G+  +    +D+ L   
Sbjct: 614 FGSYPGSPLAHHLLPNSLVSPCSPMRRGEVNMRYPSATRNYAGGV--MGSWHMDASLDE- 673

Query: 674 GHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETANVEEKTKIFPEII 733
           G G ++L+  +  +      + I         DQYGSRFIQQKLETA  +EK  ++ EI+
Sbjct: 674 GFGSSMLEEFKSNKTRGFELAEIAGHVVEFSSDQYGSRFIQQKLETATSDEKNMVYEEIM 733

Query: 734 PHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVD 793
           PHA  LMTDVFGNYVIQKFFEHG   QR+ELAD+L  +VLPLSLQMYGCRVIQKA+EVVD
Sbjct: 734 PHALALMTDVFGNYVIQKFFEHGLPPQRRELADKLFDNVLPLSLQMYGCRVIQKAIEVVD 793

Query: 794 SDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYG 853
            DQ+ +MV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F+G V+ LSTHPYG
Sbjct: 794 LDQKIKMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGNVVTLSTHPYG 853

Query: 854 CRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKL 913
           CRVIQRVLEHC D +TQ  +MDEIM ++ +L+QDQYGNYVIQHVLE GKP ER+ II +L
Sbjct: 854 CRVIQRVLEHCHDPDTQSKVMDEIMSTISMLAQDQYGNYVIQHVLEHGKPDERTVIIKEL 913

Query: 914 AGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQ 939
           AG+IV+MSQQKFASNVVEKCLTFG PEER+ L+NE+LG+TDENEPLQAMMKD F NYVVQ
Sbjct: 914 AGKIVQMSQQKFASNVVEKCLTFGGPEEREFLVNEMLGTTDENEPLQAMMKDQFANYVVQ 957

BLAST of Lsi04G000820 vs. ExPASy Swiss-Prot
Match: Q9ZW02 (Pumilio homolog 3 OS=Arabidopsis thaliana OX=3702 GN=APUM3 PE=1 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 1.8e-179
Identity = 423/1018 (41.55%), Postives = 553/1018 (54.32%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN+YRSGSAPPTV+GSL+A G
Sbjct: 15  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLYRSGSAPPTVDGSLSAAG 74

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 75  GLFSGGGGASFLEFGGVNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 134

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N S SLFSM PG                    G
Sbjct: 135 RVAQRLKGSNNVLGGVGD---RRKV--NDSRSLFSMPPGFEA-----------------G 194

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
           K  P  + SEW   G  G       GLG ++KSFADI Q  +G    +  Q SRPAS N+
Sbjct: 195 K--PGASASEWDANGLIGL------GLGGKQKSFADIFQADMGH--PVVQQPSRPASRNT 254

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  + +     S+LSR+
Sbjct: 255 FD--ENVDSNNNLSPSASQGIG--------------------APSPYCYAAVLGSSLSRN 314

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS RV   +K+N +     NG ++G  E  D    +SGL+L
Sbjct: 315 GTPDPQGIARVPSPCLTPIGSGRVSSNDKRNTSNQSPFNGVTSGLNESSDLVNALSGLNL 374

Query: 374 SSIRPEDGVNGVQSRLQL--DLGEQSDFLINMSNG---IHTRTLPELSDKKLSNPS-DNI 433
           S      G  G+  R Q   D+ +  +++  + +G   ++    P  SD+     S  N 
Sbjct: 375 S------GTGGLDERGQAEQDVEKVRNYMFGLQDGHNEVNPHGFPNRSDQARGTASCRNS 434

Query: 434 DLARKSGIVVNPRASTMSSHDHVNFP-----------------------------KRTSS 493
            +    G   N  +   + + H + P                               T  
Sbjct: 435 QMRGSQGSAYNSGSGVANPYQHHDSPNYYALNPAVASMMANQLGTNNYSPMYENASATLG 494

Query: 494 FTSLYSKPNSSGFVSKDGPTRHLQN----ANLQNVDLTGYTSGDFSINMNHSSAMNSYGA 553
           ++++ S+ +   FVS        +N     N      TG+ S   +  M H  A  S  A
Sbjct: 495 YSAMDSRLHGGSFVSSGQNLSESRNIGRVGNRMMEGGTGHPS-HLADPMYHQYARFSENA 554

Query: 554 SDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGN 613
                L   + DR             YG S  ++  ++ AY    L  QK  Y L    +
Sbjct: 555 DSFDLLNDPSMDR------------NYGNSYMNMLEIQRAY----LGSQKSQYGLPYK-S 614

Query: 614 SSVYNHRLYTNTPYGSGIPY----LADQALDSGLSS------------------------ 673
            S  +H  Y +  +GS + Y    LA   + + L S                        
Sbjct: 615 GSPNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRGEVNMRYPAATRNYTGG 674

Query: 674 ------------VGHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETA 733
                        G G ++L+  +  +      S I         DQYGSRFIQQKLETA
Sbjct: 675 VMGSWHMDASLDEGFGSSMLEEFKSNKTRGFELSEIAGHVVEFSSDQYGSRFIQQKLETA 734

Query: 734 NVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMY 793
             +EK  ++ EI+P A  LMTDVFGNYVIQKFFEHG   QR+EL ++L  +VLPLSLQMY
Sbjct: 735 TTDEKNMVYEEIMPKALALMTDVFGNYVIQKFFEHGLPPQRRELGEKLIDNVLPLSLQMY 794

Query: 794 GCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAF 853
           GCRVIQKA+EVVD DQ+ QMV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F
Sbjct: 795 GCRVIQKAIEVVDLDQKIQMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTF 854

Query: 854 YGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEF 913
           +G V+ LSTHPYGCRVIQRVLEHC + +TQ  +M+EI+ +V +L+QDQYGNYV+QHVLE 
Sbjct: 855 FGHVVTLSTHPYGCRVIQRVLEHCHNPDTQSKVMEEILSTVSMLTQDQYGNYVVQHVLEH 914

Query: 914 GKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQ 939
           GKP ER+ II +LAG+IV+MSQQKFASNVVEKCLTFG PEER+LL+NE+LG+TDENEPLQ
Sbjct: 915 GKPDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFGGPEERELLVNEMLGTTDENEPLQ 949

BLAST of Lsi04G000820 vs. ExPASy Swiss-Prot
Match: Q9LJX4 (Pumilio homolog 5 OS=Arabidopsis thaliana OX=3702 GN=APUM5 PE=1 SV=2)

HSP 1 Score: 433.0 bits (1112), Expect = 9.3e-120
Identity = 336/938 (35.82%), Postives = 449/938 (47.87%), Query Frame = 0

Query: 55  RSGSAPPTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYI 114
           RS SAPP++EGS  A+ +L      S     S + +   SE+EIRS P Y++YY SN  +
Sbjct: 59  RSESAPPSMEGSFAALRNLLKQQEGSSSEVLSRAIENYDSEEEIRSDPAYVAYYLSNINL 118

Query: 115 NPRLPPPLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTD 174
           NPRLPPPL+S+E+  + + F  +  S       +W  +      SSL S +   S  R  
Sbjct: 119 NPRLPPPLISRENQHLLRHFGDNNQS----PTTSWDNM---GIRSSLHSSRTALSTHR-- 178

Query: 175 KNNSNVMEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESA 234
                                  E  D    G   S  + L  RRKS AD++QE      
Sbjct: 179 ----------------------EEPEDEASSGEQQSYAS-LAGRRKSIADMIQEDF--PL 238

Query: 235 SLSGQLSRPAS----------HNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFV 294
           +LS    RP S          H    D  +     + P    N +  + +  +       
Sbjct: 239 TLSSVFKRPHSAGNRPIAQDIHAISSDTSSEHARRL-PESDINSVNLLRETDSLSSDAIA 298

Query: 295 GVQSHNKPV-SHSFLNPNCSTL-SRSTTPEPQLVGRSSSSGLPPVGSRVFPVEK------ 354
                   + S SF N     L +R  + E   +    +S    V SR+   ++      
Sbjct: 299 SEDPFTTDLASQSFTNAQTERLNARQASHEDNNLSVFGASPPSSVASRMRRNQEDQQSQG 358

Query: 355 ----KNITVSKVQ-NGYSAGFTELPDISGLH-LSSIRPEDGVNGVQSRLQLDLGEQSDFL 414
                  T S  Q    S      P I G   +    P+     V S  Q   G     +
Sbjct: 359 RRMPPQYTPSSYQVQASSPQQMSYPRIGGTQDMMQSLPKIATGEVHSTFQSPHGLAPPPM 418

Query: 415 INMSNGIHTRTLP----ELSDKKLSNPSDNI-DLARKSGIVVNPRASTMSSHDHVNFPKR 474
              +    T   P          +  P  N       SGIV    +   S    V  P  
Sbjct: 419 YTSTAAYMTSLSPFYHQNFQSSGMFVPQYNYGGYPPASGIVPQYMSGYPSHEATVPMPYD 478

Query: 475 TSSFTSLYSKPNSSGFVSKDGPT----------RHLQNANL--------QNVDLTGYTSG 534
            SS +S Y+ P     VS  G            ++ Q A +         + D  G    
Sbjct: 479 ISSTSSGYNNPRLLPGVSSSGQNIPSLVDPFQLQYFQQAQVDAYAPPFQSSTDSFGQKDQ 538

Query: 535 DFSINMNHSSAMNS-----YG------ASDHIKLPSGTSDRANHAGSSLQPHNYYGISQG 594
                M +   +NS     YG        ++  +P G      + GS L       +   
Sbjct: 539 QAVGYMANHEPLNSPLSPGYGLQSPRHMGNYFAVPPGVRVMPQYPGSPLASP---VMPSS 598

Query: 595 DLQGLRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSS 654
            + G+ S +       + ++++   S N+ +Y      N    S I  + D    S L  
Sbjct: 599 PVGGMMSHFGR---RSETRYHQQGPSRNTGIYPGGWQGNRGGASSI--VDDLKRHSFLDE 658

Query: 655 VGHGGTVLQNERILRFNSMMRSSIGAQGMDQYGSRFIQQKLETANVEEKTKIFPEIIPHA 714
           +        N R L   S +   +    +DQ+GSRFIQQKLE  + EEK  +F E++P A
Sbjct: 659 LKS-----PNARKLEL-SDIAGRVVEFSVDQHGSRFIQQKLEHCSDEEKASVFSEVLPQA 718

Query: 715 RTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQ 774
             LMTDVFGNYVIQKF EHGT  QR+EL  QL+G ++ LSLQMYGCRVIQKALEV+D DQ
Sbjct: 719 SKLMTDVFGNYVIQKFIEHGTPAQREELVKQLAGQMVSLSLQMYGCRVIQKALEVIDVDQ 778

Query: 775 QTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRV 834
           +T+++ ELDG+V+KCVRDQNGNHVIQKCIE +P  RI F+I+AF GQV  LSTHPYGCRV
Sbjct: 779 KTELIRELDGNVLKCVRDQNGNHVIQKCIESMPAGRIGFVIAAFRGQVATLSTHPYGCRV 838

Query: 835 IQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQ 894
           IQR+LEHC D      I+DEI++S   L+ DQYGNYV QHVLE GKP ER  II KL G 
Sbjct: 839 IQRILEHCSDDEETHCIIDEILESAFALAHDQYGNYVTQHVLERGKPDERRQIIEKLTGN 898

Query: 895 IVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVL 935
           +V+MSQ K+ASNVVEKCL      ER+ LI EI+G ++E+  L AMMKD F NYVVQKVL
Sbjct: 899 VVQMSQHKYASNVVEKCLEHADSTEREFLIEEIMGKSEEDNHLLAMMKDQFANYVVQKVL 947

BLAST of Lsi04G000820 vs. ExPASy TrEMBL
Match: A0A0A0KRK1 (PUM-HD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622680 PE=4 SV=1)

HSP 1 Score: 1755.3 bits (4545), Expect = 0.0e+00
Identity = 898/1024 (87.70%), Postives = 920/1024 (89.84%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           M SGSNTDMLSIPNE HQVP GNFEDNLR+ELELL+RENSNHSVAGR GDLNIYRSGSAP
Sbjct: 1   MVSGSNTDMLSIPNEGHQVPAGNFEDNLRTELELLLRENSNHSVAGRGGDLNIYRSGSAP 60

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGS+NAVGSLFTSSYY+EFNTKSGSNDGVLSEDEIRSHPDYLSYYYSND+INPRLPP
Sbjct: 61  PTVEGSINAVGSLFTSSYYNEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDHINPRLPP 120

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQG GSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNN NV
Sbjct: 121 PLVSKEDWRVAQRFQGIGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNGNV 180

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
           MEFGDANGKNLPRK LSEWHDRGREGFVGS GNGLGARRKSFADIVQEGLGESAS+SGQL
Sbjct: 181 MEFGDANGKNLPRKTLSEWHDRGREGFVGSGGNGLGARRKSFADIVQEGLGESASMSGQL 240

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHNSFGDVDNMGM DIKPPGLCNG+GPIED+H  GPPGFVGVQSHNK  SHSFLNP
Sbjct: 241 SRPASHNSFGDVDNMGMNDIKPPGLCNGVGPIEDMHTPGPPGFVGVQSHNKAASHSFLNP 300

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCSTLSRSTTPEPQLVGRS S GLPPVGSRVF VEKKNIT SKVQNGYSAGFTELPDISG
Sbjct: 301 NCSTLSRSTTPEPQLVGRSLSFGLPPVGSRVFAVEKKNITSSKVQNGYSAGFTELPDISG 360

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLSSIR EDGVNG QSRLQLDLGEQSDFLINM NGI  RTLP+LSDKKLS PSDNIDL 
Sbjct: 361 LHLSSIRHEDGVNGAQSRLQLDLGEQSDFLINMPNGILPRTLPDLSDKKLSKPSDNIDLT 420

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           RKSGIV+NPRASTMSSHDHVNFPKRTSS T+LYSKPNSSGFVSKDGPTRHLQNANLQ+VD
Sbjct: 421 RKSGIVMNPRASTMSSHDHVNFPKRTSSSTNLYSKPNSSGFVSKDGPTRHLQNANLQSVD 480

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GY SGDFS+NMNHSSAMNSYG SDHIKLPSGTSDR NHAGSSLQPHNYYGISQGDLQG
Sbjct: 481 LAGYPSGDFSMNMNHSSAMNSYGTSDHIKLPSGTSDRPNHAGSSLQPHNYYGISQGDLQG 540

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600

Query: 601 GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
           GT+LQNERILRFNSMMR+SIGA G                                    
Sbjct: 601 GTMLQNERILRFNSMMRTSIGAHGSWQPEIGNTADRSFPSTLLDEFKSNKTRSFELSDIV 660

Query: 661 -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                  MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 661 DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720

Query: 721 VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
            +QRKELA+QLSGHVLPLSLQMYGCRVIQKALEVVDS+QQTQMVAELDGS+MKCVRDQNG
Sbjct: 721 ASQRKELAEQLSGHVLPLSLQMYGCRVIQKALEVVDSEQQTQMVAELDGSIMKCVRDQNG 780

Query: 781 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
           NHVIQKCIECIPQ+RIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI
Sbjct: 781 NHVIQKCIECIPQERIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840

Query: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
           MQSVCLL+QDQYGNYVIQHVLEFGKPHERS IISKLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 841 MQSVCLLAQDQYGNYVIQHVLEFGKPHERSVIISKLAGQIVKMSQQKFASNVVEKCLTFG 900

Query: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960
           SPEERQLL+NEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS
Sbjct: 901 SPEERQLLVNEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960

Query: 961 LKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQSS-SS 981
           LKRYTYGKHIVSRVEKLITTG                            ERRIGQSS SS
Sbjct: 961 LKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQSSTSS 996

BLAST of Lsi04G000820 vs. ExPASy TrEMBL
Match: A0A1S4DWZ8 (pumilio homolog 4 OS=Cucumis melo OX=3656 GN=LOC103489451 PE=4 SV=1)

HSP 1 Score: 1750.3 bits (4532), Expect = 0.0e+00
Identity = 898/1024 (87.70%), Postives = 917/1024 (89.55%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           M SGSNTDMLSIP+E HQVPLGNFEDNLR+ELELL+RENSNHSV GRDGDLNIYRSGSAP
Sbjct: 2   MVSGSNTDMLSIPDEGHQVPLGNFEDNLRTELELLLRENSNHSVGGRDGDLNIYRSGSAP 61

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGS+NAVGSLFTSSYY+EFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 62  PTVEGSINAVGSLFTSSYYNEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 121

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQG GSSLGRQGDWNWKK VDGNTSSSLFSMQPGSSVQRTDKNN N 
Sbjct: 122 PLVSKEDWRVAQRFQGIGSSLGRQGDWNWKKSVDGNTSSSLFSMQPGSSVQRTDKNNGNA 181

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
           MEFGDANGKNLPRK LSEWHDRGREGFVGS  NGLGARRKSFADIVQEGLGESAS+SGQL
Sbjct: 182 MEFGDANGKNLPRKTLSEWHDRGREGFVGSGSNGLGARRKSFADIVQEGLGESASMSGQL 241

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHNSFGDVDNMGM DIKPPGL NG+GP+EDLH  GPPGFVGVQSHNK  SHSFLNP
Sbjct: 242 SRPASHNSFGDVDNMGMNDIKPPGLGNGVGPVEDLHTPGPPGFVGVQSHNKAASHSFLNP 301

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCSTLSRSTTPEPQLVGRSSS GLPPVGSRVF VEKKN+T SKVQNGYSAGFTELPDISG
Sbjct: 302 NCSTLSRSTTPEPQLVGRSSSFGLPPVGSRVFAVEKKNVTASKVQNGYSAGFTELPDISG 361

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLSSIR EDGV G QSRLQLDLGEQSDFLINMSNGI  RTLPELSDKKLS PSDNIDL 
Sbjct: 362 LHLSSIRHEDGVKGAQSRLQLDLGEQSDFLINMSNGILPRTLPELSDKKLSKPSDNIDLT 421

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           RKSGIV+NPRASTM SHDHVNFPKRTSS T+LYSKPNSSGFVSKDGPTRHLQNANLQ+VD
Sbjct: 422 RKSGIVMNPRASTMRSHDHVNFPKRTSSSTNLYSKPNSSGFVSKDGPTRHLQNANLQSVD 481

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GY SGDFS+NMNHSSAMNSYG SDHIKLPSGTSDRA+HAGSSLQPHNYYGISQGDLQG
Sbjct: 482 LAGYPSGDFSMNMNHSSAMNSYGTSDHIKLPSGTSDRASHAGSSLQPHNYYGISQGDLQG 541

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LRSAYLEALLAQQKQHYELS SGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 542 LRSAYLEALLAQQKQHYELSHSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 601

Query: 601 GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
           GT+LQNERILRFNSMMRSSIGA G                                    
Sbjct: 602 GTMLQNERILRFNSMMRSSIGAHGSWQPDIGNTVDRSFPSTLLDEFKSNKTRSFELSDIV 661

Query: 661 -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                  MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 662 DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 721

Query: 721 VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
            +QRKELA+QLSGHVLPLSLQMYGCRVIQKALEVVDS+QQTQMVAELDGS+MKCVRDQNG
Sbjct: 722 ASQRKELAEQLSGHVLPLSLQMYGCRVIQKALEVVDSEQQTQMVAELDGSIMKCVRDQNG 781

Query: 781 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
           NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDEI
Sbjct: 782 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDLNTQQIIMDEI 841

Query: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
           MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 842 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 901

Query: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960
           SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS
Sbjct: 902 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 961

Query: 961 LKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQ-SSSS 981
           LKRYTYGKHIVSRVEKLITTG                            ERRIGQ SSSS
Sbjct: 962 LKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQSSSSS 997

BLAST of Lsi04G000820 vs. ExPASy TrEMBL
Match: A0A6J1CBK8 (pumilio homolog 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010022 PE=4 SV=1)

HSP 1 Score: 1675.2 bits (4337), Expect = 0.0e+00
Identity = 854/1000 (85.40%), Postives = 890/1000 (89.00%), Query Frame = 0

Query: 1    MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
            MASGSNTDMLSIPNE HQVP+GNFEDNLR+ELELL+RENSNHS  GRDGDLNIYRSGSAP
Sbjct: 1    MASGSNTDMLSIPNEGHQVPIGNFEDNLRTELELLLRENSNHSATGRDGDLNIYRSGSAP 60

Query: 61   PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
            PTVEGSL+AVGSLFTSSY++EFN K  +ND VLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61   PTVEGSLSAVGSLFTSSYFNEFNAKGSNNDRVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121  PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
            PLVSKEDWRVAQRFQGSGSS GR GDWNWKKL DGNTS SLFSMQPG SVQR +K NSNV
Sbjct: 121  PLVSKEDWRVAQRFQGSGSSSGRHGDWNWKKLGDGNTSPSLFSMQPGPSVQRAEKINSNV 180

Query: 181  MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
             EFGDANGKNL RKALSEWHDRGR+G VGS  NGLGARRKSFA+IVQEGLGESASLSGQL
Sbjct: 181  TEFGDANGKNLSRKALSEWHDRGRDGLVGSYSNGLGARRKSFANIVQEGLGESASLSGQL 240

Query: 241  SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
            SRPASHNSFGDVD MG+ DIKPPGLCNG+GPIEDLHNSGPPGFVGVQSHNK +SHSFLNP
Sbjct: 241  SRPASHNSFGDVDTMGIADIKPPGLCNGVGPIEDLHNSGPPGFVGVQSHNKAMSHSFLNP 300

Query: 301  NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
            NCS LSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNIT+SKVQNG+SAGFTELPDISG
Sbjct: 301  NCSPLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITLSKVQNGHSAGFTELPDISG 360

Query: 361  LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
            LHLS+IRPEDGVNGVQS+LQLDLGEQS+FLINMSNGIHTR  PE SDKKLS PSD IDLA
Sbjct: 361  LHLSAIRPEDGVNGVQSQLQLDLGEQSNFLINMSNGIHTRARPEFSDKKLSKPSDYIDLA 420

Query: 421  RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
            R SGIV+NPRA TMSSHDHVNFPKRTSS TSLYSK NSSGF SK+GPTRHL NAN+Q+VD
Sbjct: 421  RTSGIVMNPRAPTMSSHDHVNFPKRTSSSTSLYSKANSSGFGSKEGPTRHLLNANVQSVD 480

Query: 481  LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
            L GYTSGD ++NMNHSSA+N Y ASDHIKL SG+SDRA HAGS LQPHNY+ IS+GDL G
Sbjct: 481  LAGYTSGDLAMNMNHSSAVNGYAASDHIKLSSGSSDRAIHAGSCLQPHNYFDISRGDLHG 540

Query: 541  LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
            LR+AYLEALLAQQKQHYELSLSG SS YNHRLY N PYG+GIPYLADQ LDSGLSSVGHG
Sbjct: 541  LRNAYLEALLAQQKQHYELSLSGKSSAYNHRLYANPPYGTGIPYLADQVLDSGLSSVGHG 600

Query: 601  GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
            G +L NER+LRFNS MR+SIGA G                                    
Sbjct: 601  GAMLHNERLLRFNSTMRNSIGAHGSWQPDIGNNADRSFPSTLLDEFKSNKTRSFELSDIV 660

Query: 661  -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                   MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 661  DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720

Query: 721  VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
              QRKELADQLS HVLPLSLQMYGCRVIQKALEVVD DQQTQMVAELDGSVMKCVRDQNG
Sbjct: 721  EIQRKELADQLSEHVLPLSLQMYGCRVIQKALEVVDLDQQTQMVAELDGSVMKCVRDQNG 780

Query: 781  NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
            NHVIQKCIEC+PQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDEI
Sbjct: 781  NHVIQKCIECVPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDVNTQQIIMDEI 840

Query: 841  MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
            MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAII+KLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 841  MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIINKLAGQIVKMSQQKFASNVVEKCLTFG 900

Query: 901  SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 956
            SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDD SLELILSRIRVHLNS
Sbjct: 901  SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDQSLELILSRIRVHLNS 960

BLAST of Lsi04G000820 vs. ExPASy TrEMBL
Match: A0A6J1HTX6 (pumilio homolog 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466545 PE=4 SV=1)

HSP 1 Score: 1669.1 bits (4321), Expect = 0.0e+00
Identity = 857/1001 (85.61%), Postives = 893/1001 (89.21%), Query Frame = 0

Query: 1    MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
            MASGSNTDML I N+ HQVPLGNFEDNLR+ELELL+RENSN SV G DGDLNIYRSGSAP
Sbjct: 1    MASGSNTDMLPIMNDGHQVPLGNFEDNLRTELELLLRENSNQSVTGEDGDLNIYRSGSAP 60

Query: 61   PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
            PTVEGSLNAVGSLFTSSYYSE N KS +ND VLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61   PTVEGSLNAVGSLFTSSYYSELNAKSSTNDRVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121  PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
            PLVSKEDWRVAQRFQGSGSSLGRQ DWNWKKLVDGNT SSLFSMQPGSSVQR +KN+SNV
Sbjct: 121  PLVSKEDWRVAQRFQGSGSSLGRQEDWNWKKLVDGNTRSSLFSMQPGSSVQRAEKNSSNV 180

Query: 181  MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
            MEFG ANG N+ R ALSEWHDRGR+G VGSCG+GLGARRKSFADIVQEGLGESASLSGQL
Sbjct: 181  MEFGGANGNNVSRNALSEWHDRGRDGLVGSCGDGLGARRKSFADIVQEGLGESASLSGQL 240

Query: 241  SRPASHNSFGDVDNMGMTDIKPPGLCNGL-GPIEDLHNSGPPGFVGVQSHNKPVSHSFLN 300
            SRPASHNSFGDVD MGMTDIKP GLCNG+ G IEDL NSGPPGFVGVQSHNK +SHSFLN
Sbjct: 241  SRPASHNSFGDVDTMGMTDIKPLGLCNGVGGSIEDLLNSGPPGFVGVQSHNKAISHSFLN 300

Query: 301  PNCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDIS 360
            PNCS LSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITV KVQNG+SAGFTE PDIS
Sbjct: 301  PNCSALSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVFKVQNGHSAGFTEPPDIS 360

Query: 361  GLHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDL 420
            GLHLSSIRPEDGVNGVQSRL LD GEQSDFLIN+SNGIHTRTLPE SDK LS PSDNIDL
Sbjct: 361  GLHLSSIRPEDGVNGVQSRLHLDHGEQSDFLINISNGIHTRTLPEFSDKNLSQPSDNIDL 420

Query: 421  ARKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNV 480
            ARKSGIV+N RA TM SHD+VNFPKRTSS TSLY+K NSSGF SK+GPT HLQN NLQ++
Sbjct: 421  ARKSGIVMNLRAPTMHSHDNVNFPKRTSSSTSLYTKANSSGFGSKEGPTIHLQNPNLQSM 480

Query: 481  DLTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQ 540
            DL GYTSGD +INMNH+SA+NSYG SDHIKLPSGTSDRA HAGSSLQ HNYYGI+QGDLQ
Sbjct: 481  DLAGYTSGDLAINMNHNSALNSYGTSDHIKLPSGTSDRAVHAGSSLQSHNYYGITQGDLQ 540

Query: 541  GLRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGH 600
            GLRSAYLE LL+QQKQHYELSLSGNSSVYNHRLY NT YGSGIPYLADQ LDSGLSS+GH
Sbjct: 541  GLRSAYLETLLSQQKQHYELSLSGNSSVYNHRLYANTLYGSGIPYLADQVLDSGLSSLGH 600

Query: 601  GGTVLQNERILRFNSMMRSSIGAQG----------------------------------- 660
            GGT+LQNERILR NS+MR+SIGAQG                                   
Sbjct: 601  GGTMLQNERILRCNSLMRNSIGAQGSWQPDIGSSVDESFPSTLLDEFKSNKTRSFELSDI 660

Query: 661  --------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720
                    MDQYGSRFIQQKLETANV EKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHG
Sbjct: 661  VDHVIEFSMDQYGSRFIQQKLETANVVEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720

Query: 721  TVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQN 780
            T +QRKELADQ+SGHVLPLSLQMYGCRVIQKALEVV SDQQ QMVAELDG VMKCVRDQN
Sbjct: 721  TESQRKELADQVSGHVLPLSLQMYGCRVIQKALEVVGSDQQAQMVAELDGLVMKCVRDQN 780

Query: 781  GNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDE 840
            GNHVIQKCIEC+PQDRIQFIIS+FYGQVLALSTHPYGCRVIQRVLEHC+D +TQQIIMDE
Sbjct: 781  GNHVIQKCIECVPQDRIQFIISSFYGQVLALSTHPYGCRVIQRVLEHCNDLSTQQIIMDE 840

Query: 841  IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTF 900
            IMQSVCLLSQDQYGNYVIQHVL+FGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTF
Sbjct: 841  IMQSVCLLSQDQYGNYVIQHVLQFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTF 900

Query: 901  GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 956
            GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN
Sbjct: 901  GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 960

BLAST of Lsi04G000820 vs. ExPASy TrEMBL
Match: A0A6J1CB96 (pumilio homolog 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010022 PE=4 SV=1)

HSP 1 Score: 1668.3 bits (4319), Expect = 0.0e+00
Identity = 858/1024 (83.79%), Postives = 893/1024 (87.21%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           MASGSNTDMLSIPNE HQVP+GNFEDNLR+ELELL+RENSNHS  GRDGDLNIYRSGSAP
Sbjct: 1   MASGSNTDMLSIPNEGHQVPIGNFEDNLRTELELLLRENSNHSATGRDGDLNIYRSGSAP 60

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGSL+AVGSLFTSSY++EFN K  +ND VLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61  PTVEGSLSAVGSLFTSSYFNEFNAKGSNNDRVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQGSGSS GR GDWNWKKL DGNTS SLFSMQPG SVQR +K NSNV
Sbjct: 121 PLVSKEDWRVAQRFQGSGSSSGRHGDWNWKKLGDGNTSPSLFSMQPGPSVQRAEKINSNV 180

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
            EFGDANGKNL RKALSEWHDRGR+G VGS  NGLGARRKSFA+IVQEGLGESASLSGQL
Sbjct: 181 TEFGDANGKNLSRKALSEWHDRGRDGLVGSYSNGLGARRKSFANIVQEGLGESASLSGQL 240

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHNSFGDVD MG+ DIKPPGLCNG+GPIEDLHNSGPPGFVGVQSHNK +SHSFLNP
Sbjct: 241 SRPASHNSFGDVDTMGIADIKPPGLCNGVGPIEDLHNSGPPGFVGVQSHNKAMSHSFLNP 300

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCS LSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNIT+SKVQNG+SAGFTELPDISG
Sbjct: 301 NCSPLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITLSKVQNGHSAGFTELPDISG 360

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLS+IRPEDGVNGVQS+LQLDLGEQS+FLINMSNGIHTR  PE SDKKLS PSD IDLA
Sbjct: 361 LHLSAIRPEDGVNGVQSQLQLDLGEQSNFLINMSNGIHTRARPEFSDKKLSKPSDYIDLA 420

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           R SGIV+NPRA TMSSHDHVNFPKRTSS TSLYSK NSSGF SK+GPTRHL NAN+Q+VD
Sbjct: 421 RTSGIVMNPRAPTMSSHDHVNFPKRTSSSTSLYSKANSSGFGSKEGPTRHLLNANVQSVD 480

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GYTSGD ++NMNHSSA+N Y ASDHIKL SG+SDRA HAGS LQPHNY+ IS+GDL G
Sbjct: 481 LAGYTSGDLAMNMNHSSAVNGYAASDHIKLSSGSSDRAIHAGSCLQPHNYFDISRGDLHG 540

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LR+AYLEALLAQQKQHYELSLSG SS YNHRLY N PYG+GIPYLADQ LDSGLSSVGHG
Sbjct: 541 LRNAYLEALLAQQKQHYELSLSGKSSAYNHRLYANPPYGTGIPYLADQVLDSGLSSVGHG 600

Query: 601 GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
           G +L NER+LRFNS MR+SIGA G                                    
Sbjct: 601 GAMLHNERLLRFNSTMRNSIGAHGSWQPDIGNNADRSFPSTLLDEFKSNKTRSFELSDIV 660

Query: 661 -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                  MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 661 DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720

Query: 721 VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
             QRKELADQLS HVLPLSLQMYGCRVIQKALEVVD DQQTQMVAELDGSVMKCVRDQNG
Sbjct: 721 EIQRKELADQLSEHVLPLSLQMYGCRVIQKALEVVDLDQQTQMVAELDGSVMKCVRDQNG 780

Query: 781 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
           NHVIQKCIEC+PQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDEI
Sbjct: 781 NHVIQKCIECVPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDVNTQQIIMDEI 840

Query: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
           MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAII+KLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIINKLAGQIVKMSQQKFASNVVEKCLTFG 900

Query: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960
           SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDD SLELILSRIRVHLNS
Sbjct: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDQSLELILSRIRVHLNS 960

Query: 961 LKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQSSSSS 982
           LKRYTYGKHIVSRVEKLITTG                            ERRIGQ SSSS
Sbjct: 961 LKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQ-SSSS 995

BLAST of Lsi04G000820 vs. NCBI nr
Match: XP_038891882.1 (pumilio homolog 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1818.9 bits (4710), Expect = 0.0e+00
Identity = 928/1028 (90.27%), Postives = 953/1028 (92.70%), Query Frame = 0

Query: 1    MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
            MASGSNTDMLSIP+E HQVPLGNFEDNLR+ELELL+RENSN SVAGRDGDLNIYRSGSAP
Sbjct: 1    MASGSNTDMLSIPDEGHQVPLGNFEDNLRTELELLLRENSNQSVAGRDGDLNIYRSGSAP 60

Query: 61   PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
            PTVEGSLNAVGSLF SSYY+E NTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61   PTVEGSLNAVGSLFRSSYYNELNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121  PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
            PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKN+SN+
Sbjct: 121  PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNSSNL 180

Query: 181  MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
            MEFGDA GKNL RKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL
Sbjct: 181  MEFGDAKGKNLSRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240

Query: 241  SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
            SRPASHN+FGDVDNMGMTDIKPPGLCNG+ PIEDLHNSGPPGFVGVQSHNK VSHSFLNP
Sbjct: 241  SRPASHNNFGDVDNMGMTDIKPPGLCNGVDPIEDLHNSGPPGFVGVQSHNKAVSHSFLNP 300

Query: 301  NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
            NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG
Sbjct: 301  NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360

Query: 361  LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
            LHLSS RP+DGVNGVQSRLQLDLGEQSDF+IN+SNGIHTRTLPELSDKKLS PSDNIDLA
Sbjct: 361  LHLSSTRPKDGVNGVQSRLQLDLGEQSDFMINLSNGIHTRTLPELSDKKLSKPSDNIDLA 420

Query: 421  RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
            RKSGIV+NPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVS DGPTRHLQNANLQ+VD
Sbjct: 421  RKSGIVMNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSSDGPTRHLQNANLQSVD 480

Query: 481  LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
            L GYTSGDFSINMNH+SAMNSYG SDHIKLPSGTSDRA+HAGSSLQPHNYYG+SQGDLQG
Sbjct: 481  LAGYTSGDFSINMNHNSAMNSYGTSDHIKLPSGTSDRASHAGSSLQPHNYYGVSQGDLQG 540

Query: 541  LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
            LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 541  LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600

Query: 601  GTVLQNERILRFNSMMR-SSIGAQG----------------------------------- 660
            GT+LQNERILRFNSMMR SSIGAQG                                   
Sbjct: 601  GTMLQNERILRFNSMMRSSSIGAQGSWQPDIGNNVDRSFPSTLLDEFKSNKTRSFELSDI 660

Query: 661  --------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720
                    MDQYGSRFIQQKLETANV+EKT+IFPEIIPHARTLMTDVFGNYVIQKFFEHG
Sbjct: 661  VDHVIEFSMDQYGSRFIQQKLETANVDEKTEIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720

Query: 721  TVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQN 780
            TV QRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQT+MVAELDGSVMKCVRDQN
Sbjct: 721  TVNQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTRMVAELDGSVMKCVRDQN 780

Query: 781  GNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDE 840
            GNHVIQKCIECIPQ RIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDE
Sbjct: 781  GNHVIQKCIECIPQYRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDLNTQQIIMDE 840

Query: 841  IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTF 900
            IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQI+KMSQQKFASNVVEKCLTF
Sbjct: 841  IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIIKMSQQKFASNVVEKCLTF 900

Query: 901  GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 960
            GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN
Sbjct: 901  GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 960

Query: 961  SLKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWS---QLIMLNLFAERRIGQS 982
            SLKRYTYGKHIVSRVEKLITTGGEPFF    TLDFF  V +     ++ +L AERRIGQS
Sbjct: 961  SLKRYTYGKHIVSRVEKLITTGGEPFFATPTTLDFFLGVVATHHAKLVCSLLAERRIGQS 1020

BLAST of Lsi04G000820 vs. NCBI nr
Match: XP_038891883.1 (pumilio homolog 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 915/1025 (89.27%), Postives = 936/1025 (91.32%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           MASGSNTDMLSIP+E HQVPLGNFEDNLR+ELELL+RENSN SVAGRDGDLNIYRSGSAP
Sbjct: 1   MASGSNTDMLSIPDEGHQVPLGNFEDNLRTELELLLRENSNQSVAGRDGDLNIYRSGSAP 60

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGSLNAVGSLF SSYY+E NTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61  PTVEGSLNAVGSLFRSSYYNELNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKN+SN+
Sbjct: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNSSNL 180

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
           MEFGDA GKNL RKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL
Sbjct: 181 MEFGDAKGKNLSRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHN+FGDVDNMGMTDIKPPGLCNG+ PIEDLHNSGPPGFVGVQSHNK VSHSFLNP
Sbjct: 241 SRPASHNNFGDVDNMGMTDIKPPGLCNGVDPIEDLHNSGPPGFVGVQSHNKAVSHSFLNP 300

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG
Sbjct: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLSS RP+DGVNGVQSRLQLDLGEQSDF+IN+SNGIHTRTLPELSDKKLS PSDNIDLA
Sbjct: 361 LHLSSTRPKDGVNGVQSRLQLDLGEQSDFMINLSNGIHTRTLPELSDKKLSKPSDNIDLA 420

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           RKSGIV+NPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVS DGPTRHLQNANLQ+VD
Sbjct: 421 RKSGIVMNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSSDGPTRHLQNANLQSVD 480

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GYTSGDFSINMNH+SAMNSYG SDHIKLPSGTSDRA+HAGSSLQPHNYYG+SQGDLQG
Sbjct: 481 LAGYTSGDFSINMNHNSAMNSYGTSDHIKLPSGTSDRASHAGSSLQPHNYYGVSQGDLQG 540

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600

Query: 601 GTVLQNERILRFNSMMR-SSIGAQG----------------------------------- 660
           GT+LQNERILRFNSMMR SSIGAQG                                   
Sbjct: 601 GTMLQNERILRFNSMMRSSSIGAQGSWQPDIGNNVDRSFPSTLLDEFKSNKTRSFELSDI 660

Query: 661 --------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720
                   MDQYGSRFIQQKLETANV+EKT+IFPEIIPHARTLMTDVFGNYVIQKFFEHG
Sbjct: 661 VDHVIEFSMDQYGSRFIQQKLETANVDEKTEIFPEIIPHARTLMTDVFGNYVIQKFFEHG 720

Query: 721 TVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQN 780
           TV QRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQT+MVAELDGSVMKCVRDQN
Sbjct: 721 TVNQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTRMVAELDGSVMKCVRDQN 780

Query: 781 GNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDE 840
           GNHVIQKCIECIPQ RIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDE
Sbjct: 781 GNHVIQKCIECIPQYRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDLNTQQIIMDE 840

Query: 841 IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTF 900
           IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQI+KMSQQKFASNVVEKCLTF
Sbjct: 841 IMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIIKMSQQKFASNVVEKCLTF 900

Query: 901 GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 960
           GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN
Sbjct: 901 GSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLN 960

Query: 961 SLKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQSSSS 982
           SLKRYTYGKHIVSRVEKLITTG                            ERRIGQSSSS
Sbjct: 961 SLKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQSSSS 997

BLAST of Lsi04G000820 vs. NCBI nr
Match: XP_004142495.1 (pumilio homolog 4 [Cucumis sativus] >KGN52253.1 hypothetical protein Csa_007726 [Cucumis sativus])

HSP 1 Score: 1755.3 bits (4545), Expect = 0.0e+00
Identity = 898/1024 (87.70%), Postives = 920/1024 (89.84%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           M SGSNTDMLSIPNE HQVP GNFEDNLR+ELELL+RENSNHSVAGR GDLNIYRSGSAP
Sbjct: 1   MVSGSNTDMLSIPNEGHQVPAGNFEDNLRTELELLLRENSNHSVAGRGGDLNIYRSGSAP 60

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGS+NAVGSLFTSSYY+EFNTKSGSNDGVLSEDEIRSHPDYLSYYYSND+INPRLPP
Sbjct: 61  PTVEGSINAVGSLFTSSYYNEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDHINPRLPP 120

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQG GSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNN NV
Sbjct: 121 PLVSKEDWRVAQRFQGIGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNGNV 180

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
           MEFGDANGKNLPRK LSEWHDRGREGFVGS GNGLGARRKSFADIVQEGLGESAS+SGQL
Sbjct: 181 MEFGDANGKNLPRKTLSEWHDRGREGFVGSGGNGLGARRKSFADIVQEGLGESASMSGQL 240

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHNSFGDVDNMGM DIKPPGLCNG+GPIED+H  GPPGFVGVQSHNK  SHSFLNP
Sbjct: 241 SRPASHNSFGDVDNMGMNDIKPPGLCNGVGPIEDMHTPGPPGFVGVQSHNKAASHSFLNP 300

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCSTLSRSTTPEPQLVGRS S GLPPVGSRVF VEKKNIT SKVQNGYSAGFTELPDISG
Sbjct: 301 NCSTLSRSTTPEPQLVGRSLSFGLPPVGSRVFAVEKKNITSSKVQNGYSAGFTELPDISG 360

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLSSIR EDGVNG QSRLQLDLGEQSDFLINM NGI  RTLP+LSDKKLS PSDNIDL 
Sbjct: 361 LHLSSIRHEDGVNGAQSRLQLDLGEQSDFLINMPNGILPRTLPDLSDKKLSKPSDNIDLT 420

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           RKSGIV+NPRASTMSSHDHVNFPKRTSS T+LYSKPNSSGFVSKDGPTRHLQNANLQ+VD
Sbjct: 421 RKSGIVMNPRASTMSSHDHVNFPKRTSSSTNLYSKPNSSGFVSKDGPTRHLQNANLQSVD 480

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GY SGDFS+NMNHSSAMNSYG SDHIKLPSGTSDR NHAGSSLQPHNYYGISQGDLQG
Sbjct: 481 LAGYPSGDFSMNMNHSSAMNSYGTSDHIKLPSGTSDRPNHAGSSLQPHNYYGISQGDLQG 540

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600

Query: 601 GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
           GT+LQNERILRFNSMMR+SIGA G                                    
Sbjct: 601 GTMLQNERILRFNSMMRTSIGAHGSWQPEIGNTADRSFPSTLLDEFKSNKTRSFELSDIV 660

Query: 661 -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                  MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 661 DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720

Query: 721 VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
            +QRKELA+QLSGHVLPLSLQMYGCRVIQKALEVVDS+QQTQMVAELDGS+MKCVRDQNG
Sbjct: 721 ASQRKELAEQLSGHVLPLSLQMYGCRVIQKALEVVDSEQQTQMVAELDGSIMKCVRDQNG 780

Query: 781 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
           NHVIQKCIECIPQ+RIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI
Sbjct: 781 NHVIQKCIECIPQERIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840

Query: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
           MQSVCLL+QDQYGNYVIQHVLEFGKPHERS IISKLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 841 MQSVCLLAQDQYGNYVIQHVLEFGKPHERSVIISKLAGQIVKMSQQKFASNVVEKCLTFG 900

Query: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960
           SPEERQLL+NEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS
Sbjct: 901 SPEERQLLVNEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960

Query: 961 LKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQSS-SS 981
           LKRYTYGKHIVSRVEKLITTG                            ERRIGQSS SS
Sbjct: 961 LKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQSSTSS 996

BLAST of Lsi04G000820 vs. NCBI nr
Match: XP_008446860.1 (PREDICTED: pumilio homolog 4 [Cucumis melo] >XP_016900250.1 PREDICTED: pumilio homolog 4 [Cucumis melo] >XP_016900251.1 PREDICTED: pumilio homolog 4 [Cucumis melo])

HSP 1 Score: 1750.3 bits (4532), Expect = 0.0e+00
Identity = 898/1024 (87.70%), Postives = 917/1024 (89.55%), Query Frame = 0

Query: 1   MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
           M SGSNTDMLSIP+E HQVPLGNFEDNLR+ELELL+RENSNHSV GRDGDLNIYRSGSAP
Sbjct: 2   MVSGSNTDMLSIPDEGHQVPLGNFEDNLRTELELLLRENSNHSVGGRDGDLNIYRSGSAP 61

Query: 61  PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
           PTVEGS+NAVGSLFTSSYY+EFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 62  PTVEGSINAVGSLFTSSYYNEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 121

Query: 121 PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
           PLVSKEDWRVAQRFQG GSSLGRQGDWNWKK VDGNTSSSLFSMQPGSSVQRTDKNN N 
Sbjct: 122 PLVSKEDWRVAQRFQGIGSSLGRQGDWNWKKSVDGNTSSSLFSMQPGSSVQRTDKNNGNA 181

Query: 181 MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
           MEFGDANGKNLPRK LSEWHDRGREGFVGS  NGLGARRKSFADIVQEGLGESAS+SGQL
Sbjct: 182 MEFGDANGKNLPRKTLSEWHDRGREGFVGSGSNGLGARRKSFADIVQEGLGESASMSGQL 241

Query: 241 SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
           SRPASHNSFGDVDNMGM DIKPPGL NG+GP+EDLH  GPPGFVGVQSHNK  SHSFLNP
Sbjct: 242 SRPASHNSFGDVDNMGMNDIKPPGLGNGVGPVEDLHTPGPPGFVGVQSHNKAASHSFLNP 301

Query: 301 NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
           NCSTLSRSTTPEPQLVGRSSS GLPPVGSRVF VEKKN+T SKVQNGYSAGFTELPDISG
Sbjct: 302 NCSTLSRSTTPEPQLVGRSSSFGLPPVGSRVFAVEKKNVTASKVQNGYSAGFTELPDISG 361

Query: 361 LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
           LHLSSIR EDGV G QSRLQLDLGEQSDFLINMSNGI  RTLPELSDKKLS PSDNIDL 
Sbjct: 362 LHLSSIRHEDGVKGAQSRLQLDLGEQSDFLINMSNGILPRTLPELSDKKLSKPSDNIDLT 421

Query: 421 RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
           RKSGIV+NPRASTM SHDHVNFPKRTSS T+LYSKPNSSGFVSKDGPTRHLQNANLQ+VD
Sbjct: 422 RKSGIVMNPRASTMRSHDHVNFPKRTSSSTNLYSKPNSSGFVSKDGPTRHLQNANLQSVD 481

Query: 481 LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
           L GY SGDFS+NMNHSSAMNSYG SDHIKLPSGTSDRA+HAGSSLQPHNYYGISQGDLQG
Sbjct: 482 LAGYPSGDFSMNMNHSSAMNSYGTSDHIKLPSGTSDRASHAGSSLQPHNYYGISQGDLQG 541

Query: 541 LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
           LRSAYLEALLAQQKQHYELS SGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG
Sbjct: 542 LRSAYLEALLAQQKQHYELSHSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 601

Query: 601 GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
           GT+LQNERILRFNSMMRSSIGA G                                    
Sbjct: 602 GTMLQNERILRFNSMMRSSIGAHGSWQPDIGNTVDRSFPSTLLDEFKSNKTRSFELSDIV 661

Query: 661 -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                  MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 662 DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 721

Query: 721 VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
            +QRKELA+QLSGHVLPLSLQMYGCRVIQKALEVVDS+QQTQMVAELDGS+MKCVRDQNG
Sbjct: 722 ASQRKELAEQLSGHVLPLSLQMYGCRVIQKALEVVDSEQQTQMVAELDGSIMKCVRDQNG 781

Query: 781 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
           NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDEI
Sbjct: 782 NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDLNTQQIIMDEI 841

Query: 841 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
           MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 842 MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 901

Query: 901 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 960
           SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS
Sbjct: 902 SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 961

Query: 961 LKRYTYGKHIVSRVEKLITTGGEPFFTRLVTLDFFPSVWSQLIMLNLFAERRIGQ-SSSS 981
           LKRYTYGKHIVSRVEKLITTG                            ERRIGQ SSSS
Sbjct: 962 LKRYTYGKHIVSRVEKLITTG----------------------------ERRIGQSSSSS 997

BLAST of Lsi04G000820 vs. NCBI nr
Match: XP_022138974.1 (pumilio homolog 4 isoform X1 [Momordica charantia] >XP_022138975.1 pumilio homolog 4 isoform X1 [Momordica charantia])

HSP 1 Score: 1675.2 bits (4337), Expect = 0.0e+00
Identity = 854/1000 (85.40%), Postives = 890/1000 (89.00%), Query Frame = 0

Query: 1    MASGSNTDMLSIPNEDHQVPLGNFEDNLRSELELLMRENSNHSVAGRDGDLNIYRSGSAP 60
            MASGSNTDMLSIPNE HQVP+GNFEDNLR+ELELL+RENSNHS  GRDGDLNIYRSGSAP
Sbjct: 1    MASGSNTDMLSIPNEGHQVPIGNFEDNLRTELELLLRENSNHSATGRDGDLNIYRSGSAP 60

Query: 61   PTVEGSLNAVGSLFTSSYYSEFNTKSGSNDGVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120
            PTVEGSL+AVGSLFTSSY++EFN K  +ND VLSEDEIRSHPDYLSYYYSNDYINPRLPP
Sbjct: 61   PTVEGSLSAVGSLFTSSYFNEFNAKGSNNDRVLSEDEIRSHPDYLSYYYSNDYINPRLPP 120

Query: 121  PLVSKEDWRVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNV 180
            PLVSKEDWRVAQRFQGSGSS GR GDWNWKKL DGNTS SLFSMQPG SVQR +K NSNV
Sbjct: 121  PLVSKEDWRVAQRFQGSGSSSGRHGDWNWKKLGDGNTSPSLFSMQPGPSVQRAEKINSNV 180

Query: 181  MEFGDANGKNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQL 240
             EFGDANGKNL RKALSEWHDRGR+G VGS  NGLGARRKSFA+IVQEGLGESASLSGQL
Sbjct: 181  TEFGDANGKNLSRKALSEWHDRGRDGLVGSYSNGLGARRKSFANIVQEGLGESASLSGQL 240

Query: 241  SRPASHNSFGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNP 300
            SRPASHNSFGDVD MG+ DIKPPGLCNG+GPIEDLHNSGPPGFVGVQSHNK +SHSFLNP
Sbjct: 241  SRPASHNSFGDVDTMGIADIKPPGLCNGVGPIEDLHNSGPPGFVGVQSHNKAMSHSFLNP 300

Query: 301  NCSTLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPDISG 360
            NCS LSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNIT+SKVQNG+SAGFTELPDISG
Sbjct: 301  NCSPLSRSTTPEPQLVGRSSSSGLPPVGSRVFPVEKKNITLSKVQNGHSAGFTELPDISG 360

Query: 361  LHLSSIRPEDGVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLA 420
            LHLS+IRPEDGVNGVQS+LQLDLGEQS+FLINMSNGIHTR  PE SDKKLS PSD IDLA
Sbjct: 361  LHLSAIRPEDGVNGVQSQLQLDLGEQSNFLINMSNGIHTRARPEFSDKKLSKPSDYIDLA 420

Query: 421  RKSGIVVNPRASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVD 480
            R SGIV+NPRA TMSSHDHVNFPKRTSS TSLYSK NSSGF SK+GPTRHL NAN+Q+VD
Sbjct: 421  RTSGIVMNPRAPTMSSHDHVNFPKRTSSSTSLYSKANSSGFGSKEGPTRHLLNANVQSVD 480

Query: 481  LTGYTSGDFSINMNHSSAMNSYGASDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQG 540
            L GYTSGD ++NMNHSSA+N Y ASDHIKL SG+SDRA HAGS LQPHNY+ IS+GDL G
Sbjct: 481  LAGYTSGDLAMNMNHSSAVNGYAASDHIKLSSGSSDRAIHAGSCLQPHNYFDISRGDLHG 540

Query: 541  LRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTPYGSGIPYLADQALDSGLSSVGHG 600
            LR+AYLEALLAQQKQHYELSLSG SS YNHRLY N PYG+GIPYLADQ LDSGLSSVGHG
Sbjct: 541  LRNAYLEALLAQQKQHYELSLSGKSSAYNHRLYANPPYGTGIPYLADQVLDSGLSSVGHG 600

Query: 601  GTVLQNERILRFNSMMRSSIGAQG------------------------------------ 660
            G +L NER+LRFNS MR+SIGA G                                    
Sbjct: 601  GAMLHNERLLRFNSTMRNSIGAHGSWQPDIGNNADRSFPSTLLDEFKSNKTRSFELSDIV 660

Query: 661  -------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720
                   MDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT
Sbjct: 661  DHVIEFSMDQYGSRFIQQKLETANVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGT 720

Query: 721  VTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNG 780
              QRKELADQLS HVLPLSLQMYGCRVIQKALEVVD DQQTQMVAELDGSVMKCVRDQNG
Sbjct: 721  EIQRKELADQLSEHVLPLSLQMYGCRVIQKALEVVDLDQQTQMVAELDGSVMKCVRDQNG 780

Query: 781  NHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEI 840
            NHVIQKCIEC+PQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDD NTQQIIMDEI
Sbjct: 781  NHVIQKCIECVPQDRIQFIISAFYGQVLALSTHPYGCRVIQRVLEHCDDVNTQQIIMDEI 840

Query: 841  MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFG 900
            MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAII+KLAGQIVKMSQQKFASNVVEKCLTFG
Sbjct: 841  MQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIINKLAGQIVKMSQQKFASNVVEKCLTFG 900

Query: 901  SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDHSLELILSRIRVHLNS 956
            SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDD SLELILSRIRVHLNS
Sbjct: 901  SPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLESCDDQSLELILSRIRVHLNS 960

BLAST of Lsi04G000820 vs. TAIR 10
Match: AT3G10360.1 (pumilio 4 )

HSP 1 Score: 804.7 bits (2077), Expect = 8.4e-233
Identity = 496/1001 (49.55%), Postives = 632/1001 (63.14%), Query Frame = 0

Query: 23  NFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVGSLFTSSYYS 82
           + ED  + ELE L++++ N    G  R+ D++++RSGSAPPTVEG L A+ + + ++  S
Sbjct: 23  DLEDRFQRELESLLQQHRNQQSFGRERERDIDVHRSGSAPPTVEGLLRAMDNQYLNNNNS 82

Query: 83  EFN-----TKSGSNDGV--LSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDWRVAQR 142
           +       +   +++GV  LS+DE+R HP+YLSYYYSN++ NPRLPPPL+S+EDWRVAQR
Sbjct: 83  DHRDVGNISSITTSNGVELLSDDELRWHPEYLSYYYSNEHSNPRLPPPLLSREDWRVAQR 142

Query: 143 FQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANGKNLPR 202
           F  S S     G+W  KK V+ + SSSLFS+QPG  V++ +   +++ME  +A  +   +
Sbjct: 143 FHNSESVFDPVGEWR-KKTVEVDNSSSLFSVQPGVPVEQAE---NDLMELRNAVAQGRSQ 202

Query: 203 KALSEWHDRGREGFVGSCG-NGLGARRKSFADIVQEGLGESASLSGQLSRPASHNSFGDV 262
           K   +  D+GRE  +G  G +GLG RRKSFADI+QEGL   A+L  QLSRPAS N+F D+
Sbjct: 203 KV--QRLDQGREDLIGLSGYSGLGPRRKSFADILQEGLERDAALGSQLSRPASCNTFRDM 262

Query: 263 -DNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRSTTP 322
            D   +++    G  + L   + LH++         S N  +  +  +P    + R+ TP
Sbjct: 263 KDAAVLSNFSAGGFDSPLAFHDSLHSTAK------NSPNTMLGSTMSSP----VPRNRTP 322

Query: 323 EPQLVGRSSSSGLPPVGSRVFPVEKKNITVSKVQNGYSAGFTELPD-ISGLHLSSIRPED 382
           +  LVGRS++SGLPP+G+RV PVEKKN   + +QN  S    ++ D +S L++S +  + 
Sbjct: 323 DSHLVGRSTASGLPPIGTRVGPVEKKNTFGTAIQNCESYTAADVADTLSRLNMSEM-SQV 382

Query: 383 GVNGVQSRLQLDLGEQSDFLINMSNGIHTRTLPELSDKKLSNPSDNIDLARKSGIVVNPR 442
             N +QS+LQ++L  QSD +  + NG H + L + +  +  +   + +    SG   +  
Sbjct: 383 KENHMQSQLQVELENQSDVMRYIPNG-HKKALRQQNTAETKDHLFSANYGGMSGYGASLG 442

Query: 443 ASTMSSHDHVNFPKRTSSFTSLYSKPNSSGFVSKDGPTRHLQNANLQNVDLTGYTSGDFS 502
           AST+ SH  VN PKRTSS  SLYS  + S   S      +++N N+   D +  T+G + 
Sbjct: 443 ASTVGSHGQVNIPKRTSSSASLYSTSDHSRLGSVGLSDVNIRNGNINGTDFS--TAGGYM 502

Query: 503 INMNHSSAMNSYGA-SDHIKLPSGTSDR------ANHAGSSL-----QPH---------- 562
                +S    Y A   H+   +G  DR       N   S L      PH          
Sbjct: 503 AKNKLNSLAEHYSAEGSHL---TGDGDRQSLNRLINQVASELHSPVMDPHYSQYLHTASS 562

Query: 563 -----------NYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSSVYNHRLYTNTP 622
                      N +G S GD     + YL  LLAQ +Q       GN +  N R + +  
Sbjct: 563 TAAPIDHSLIRNNFGTSNGD---TANEYLAMLLAQNRQQL-----GNLNAANSRFFESPS 622

Query: 623 YGSGIPYLADQALDSGLSSVGHGGTVLQNERILRFNSMMRSSIGA-QG------------ 682
           Y  G  YL +      L S        QN R+ +  SMM+   G  QG            
Sbjct: 623 YDLGNMYLGNH-----LPSPSKNSRNFQNMRMSQSASMMKVPFGGLQGSSHVDIGSTAEA 682

Query: 683 ---------------------------MDQYGSRFIQQKLETANVEEKTKIFPEIIPHAR 742
                                      MDQYGSRFIQQKLETA  EEK  IFPEI+P+ R
Sbjct: 683 SLLEGFKNNKTRSLELSEIVGHVIEFSMDQYGSRFIQQKLETATDEEKNAIFPEILPYGR 742

Query: 743 TLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVDSDQQ 802
           TLMTDVFGNYVIQKFFEHGT  QRKELA+Q++GHVL LSLQMYGCRVIQKALEVV+ +QQ
Sbjct: 743 TLMTDVFGNYVIQKFFEHGTTKQRKELAEQVTGHVLALSLQMYGCRVIQKALEVVELEQQ 802

Query: 803 TQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYGCRVI 862
            +MV ELDGSVMKCV DQNGNHVIQKCIE +PQD IQFIIS+FYG+VLALSTHPYGCRVI
Sbjct: 803 ARMVKELDGSVMKCVHDQNGNHVIQKCIERLPQDWIQFIISSFYGKVLALSTHPYGCRVI 862

Query: 863 QRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKLAGQI 922
           QRVLEH DD  TQ+IIM+EIM SVC L+QDQYGNYVIQH+++ GKPHERS II+KLAGQI
Sbjct: 863 QRVLEHIDDIETQRIIMEEIMDSVCTLAQDQYGNYVIQHIIQHGKPHERSEIINKLAGQI 922

Query: 923 VKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQKVLE 939
           VKMSQQKFASNVVEKCLTFG PEERQ+L+NE+LG TDENEPLQAMMKDPFGNYVVQKVLE
Sbjct: 923 VKMSQQKFASNVVEKCLTFGGPEERQVLVNEMLGYTDENEPLQAMMKDPFGNYVVQKVLE 982

BLAST of Lsi04G000820 vs. TAIR 10
Match: AT2G29200.1 (pumilio 1 )

HSP 1 Score: 642.1 bits (1655), Expect = 7.2e-184
Identity = 421/1016 (41.44%), Postives = 569/1016 (56.00%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN++RSGSAPPTV+GS++A G
Sbjct: 14  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLFRSGSAPPTVDGSVSAAG 73

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 74  GLFSGGGGAPFLEFGGVNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 133

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N + SLFSM PG   ++T               
Sbjct: 134 RVAQRLKGSNNVLGGVGD---RRKV--NDNRSLFSMPPGFEGEKTG-------------- 193

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
                 + SEW      G +G  G GLG ++KSFADI Q  +G    ++ Q SRPAS N+
Sbjct: 194 -----ASASEW---DANGLIGLPGLGLGGKQKSFADIFQADMGHGHPVAQQPSRPASRNT 253

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  +S+     S+LSR+
Sbjct: 254 FD--ENVDSNNNLSPSASQGIG--------------------APSPYSYAAVLGSSLSRN 313

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS R+   +K+N +     NG ++G  E  D    +SGL+L
Sbjct: 314 GTPDPQAIARVPSPCLTPIGSGRMSSNDKRNTSNQSPFNGVTSGLNESSDLVNALSGLNL 373

Query: 374 S-SIRPEDGVNGVQSRLQLDLGEQSDFLINMSNG---IHTRTLPELSDKK---------- 433
           S S+  +D     +S+ + D+ +  +++  +  G   ++    P  SD+           
Sbjct: 374 SCSVGLDD-----RSQAEQDVEKVRNYMFGLQGGHNEVNQHEFPNKSDQAHKATGSLRNS 433

Query: 434 ---------------LSNPSDNIDLAR--KSGIVVNPRASTMSSHDHVNFPKRTSSFTSL 493
                          L+NP   +D      +   +NP  ++M ++   N     ++F  +
Sbjct: 434 QLRGPHGSAYNGGVGLANPYQQLDSPNYCLNNYALNPAVASMMANQLGN-----NNFAPM 493

Query: 494 YSKPNSSGFVSKDGPTRHLQN---ANLQNVDLTGYTSGDFSINMNHSSAMNSYGASDHIK 553
           Y   ++ GF   D  +RH      ++ QN+       G FS  M    A    G   H+ 
Sbjct: 494 YDNVSALGFSGMD--SRHHGRGFVSSGQNLS-ESRNLGRFSNRMMGGGA----GLQSHMV 553

Query: 554 LP--SGTSDRANHAGSSLQPHNYY-GISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSS 613
            P  +  +D  +         N+  G S  D+  L+ AYL A    QK  Y +    + S
Sbjct: 554 DPMYNQYADSLDLLNDPSMDRNFMGGSSYMDMLELQRAYLGA----QKSQYGVPYK-SGS 613

Query: 614 VYNHRLYTNTPYGSGIPY----LADQALDSGLSS-------------------------- 673
             +H  Y +  +GS + Y    LA   + + L S                          
Sbjct: 614 PNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRDEVNMRFPSATRNYSGGLM 673

Query: 674 ----------VGHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETANV 733
                      G G ++L+  +  +      S I         DQYGSRFIQQKLETA  
Sbjct: 674 GSWHMDASFDEGFGSSMLEEFKSNKTRGFELSEIAGHVVEFSSDQYGSRFIQQKLETATT 733

Query: 734 EEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGC 793
           +EK  ++ EI+P A  LMTDVFGNYVIQKFFEHG   QR+ELA++L  HVLPLSLQMYGC
Sbjct: 734 DEKNMVYEEIMPQALVLMTDVFGNYVIQKFFEHGLPPQRRELAEKLFDHVLPLSLQMYGC 793

Query: 794 RVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYG 853
           RVIQKA+EVVD DQ+ +MV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F+G
Sbjct: 794 RVIQKAIEVVDLDQKIKMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFG 853

Query: 854 QVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGK 913
            V+ LSTHPYGCRVIQRVLEHC D +TQ  +M+EI+ +V +L+QDQYGNYV+QHVLE GK
Sbjct: 854 HVVTLSTHPYGCRVIQRVLEHCHDPDTQSKVMEEILSTVSMLAQDQYGNYVVQHVLEHGK 913

Query: 914 PHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAM 939
           P ER+ II +LAG+IV+MSQQKFASNVVEKCLTFG PEER+LL+NE+LG+TDENEPLQAM
Sbjct: 914 PDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFGGPEERELLVNEMLGTTDENEPLQAM 953

BLAST of Lsi04G000820 vs. TAIR 10
Match: AT2G29190.1 (pumilio 2 )

HSP 1 Score: 639.4 bits (1648), Expect = 4.7e-183
Identity = 415/1005 (41.29%), Postives = 560/1005 (55.72%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN+YRSGSAPPTV+GS++A G
Sbjct: 14  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLYRSGSAPPTVDGSVSAAG 73

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 74  GLFSGGGGAPFLEFGGGNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 133

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N S SLFSM PG      D+   +  E    + 
Sbjct: 134 RVAQRLKGSSNVLGGVGD---RRKV--NDSQSLFSMPPG-----FDQRKQHEFEVEKTSA 193

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
            +      SEW      G +G  G G+G ++KSFADI Q  +G    ++ Q SRPAS N+
Sbjct: 194 SS------SEW---DANGLIGLPGLGIGGKQKSFADIFQADMGHGHPVTKQPSRPASRNT 253

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  +S+     S+LSR+
Sbjct: 254 FD--ENVDSKNNLSPSASQGIG--------------------APSPYSYAAVLGSSLSRN 313

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS RV   +K+N   +  Q+ ++ G  E  D    +SG++L
Sbjct: 314 GTPDPQAIARVPSPCLTPIGSGRVSSNDKRN---TSNQSPFNGGLNESSDLVNALSGMNL 373

Query: 374 SSIRPEDGVNGVQSRLQL--DLGEQSDFLINMSNG---IHTRTLPELSD--KKLSNPSDN 433
           S      G  G+  R Q   D+ +  +++  +  G   ++    P  SD  +K +    N
Sbjct: 374 S------GSGGLDERGQAEQDVEKVRNYMFGLQGGHNEVNQHGFPNKSDQAQKATGLLRN 433

Query: 434 IDLARKSGIVVNPRASTMSSHDHVNFPK--------------------RTSSFTSLYSKP 493
             L    G   N      + + H++ P                      T++++ +Y   
Sbjct: 434 SQLRGAQGSTYNDGGGVATQYQHLDSPNYCLNNYGLNPAVASMMANQLGTNNYSPVYENA 493

Query: 494 NSSGFVSKDGPTRHLQNANL----QNVDLTGYTSGDFSINMNHSSAMNSYGASDHIKLPS 553
           +++  +   G    L         QN+  +       +  M   + + S+ A       +
Sbjct: 494 SAASAMGFSGMDSRLHGGGYVSSGQNLSESRNLGRFSNRMMGGGTGLQSHMADPMYHQYA 553

Query: 554 GTSDRANHAGSSLQPHNYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSSVYN--- 613
            + D  N     +   N+ G S  ++  L+ AYL A  +Q    Y+     + + Y    
Sbjct: 554 DSLDLLNDPSMDV---NFMGNSYMNMLELQRAYLGAQKSQYGVPYKSGSPNSHTDYGSPT 613

Query: 614 ----------HRLYTNT----------------------PYGSGIPYLADQALDSGLSSV 673
                     H L  N+                       Y  G+  +    +D+ L   
Sbjct: 614 FGSYPGSPLAHHLLPNSLVSPCSPMRRGEVNMRYPSATRNYAGGV--MGSWHMDASLDE- 673

Query: 674 GHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETANVEEKTKIFPEII 733
           G G ++L+  +  +      + I         DQYGSRFIQQKLETA  +EK  ++ EI+
Sbjct: 674 GFGSSMLEEFKSNKTRGFELAEIAGHVVEFSSDQYGSRFIQQKLETATSDEKNMVYEEIM 733

Query: 734 PHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVD 793
           PHA  LMTDVFGNYVIQKFFEHG   QR+ELAD+L  +VLPLSLQMYGCRVIQKA+EVVD
Sbjct: 734 PHALALMTDVFGNYVIQKFFEHGLPPQRRELADKLFDNVLPLSLQMYGCRVIQKAIEVVD 793

Query: 794 SDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYG 853
            DQ+ +MV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F+G V+ LSTHPYG
Sbjct: 794 LDQKIKMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGNVVTLSTHPYG 853

Query: 854 CRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKL 913
           CRVIQRVLEHC D +TQ  +MDEIM ++ +L+QDQYGNYVIQHVLE GKP ER+ II +L
Sbjct: 854 CRVIQRVLEHCHDPDTQSKVMDEIMSTISMLAQDQYGNYVIQHVLEHGKPDERTVIIKEL 913

Query: 914 AGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQ 939
           AG+IV+MSQQKFASNVVEKCLTFG PEER+ L+NE+LG+TDENEPLQAMMKD F NYVVQ
Sbjct: 914 AGKIVQMSQQKFASNVVEKCLTFGGPEEREFLVNEMLGTTDENEPLQAMMKDQFANYVVQ 957

BLAST of Lsi04G000820 vs. TAIR 10
Match: AT2G29190.2 (pumilio 2 )

HSP 1 Score: 639.4 bits (1648), Expect = 4.7e-183
Identity = 415/1005 (41.29%), Postives = 560/1005 (55.72%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN+YRSGSAPPTV+GS++A G
Sbjct: 14  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLYRSGSAPPTVDGSVSAAG 73

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 74  GLFSGGGGAPFLEFGGGNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 133

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N S SLFSM PG      D+   +  E    + 
Sbjct: 134 RVAQRLKGSSNVLGGVGD---RRKV--NDSQSLFSMPPG-----FDQRKQHEFEVEKTSA 193

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
            +      SEW      G +G  G G+G ++KSFADI Q  +G    ++ Q SRPAS N+
Sbjct: 194 SS------SEW---DANGLIGLPGLGIGGKQKSFADIFQADMGHGHPVTKQPSRPASRNT 253

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  +S+     S+LSR+
Sbjct: 254 FD--ENVDSKNNLSPSASQGIG--------------------APSPYSYAAVLGSSLSRN 313

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS RV   +K+N   +  Q+ ++ G  E  D    +SG++L
Sbjct: 314 GTPDPQAIARVPSPCLTPIGSGRVSSNDKRN---TSNQSPFNGGLNESSDLVNALSGMNL 373

Query: 374 SSIRPEDGVNGVQSRLQL--DLGEQSDFLINMSNG---IHTRTLPELSD--KKLSNPSDN 433
           S      G  G+  R Q   D+ +  +++  +  G   ++    P  SD  +K +    N
Sbjct: 374 S------GSGGLDERGQAEQDVEKVRNYMFGLQGGHNEVNQHGFPNKSDQAQKATGLLRN 433

Query: 434 IDLARKSGIVVNPRASTMSSHDHVNFPK--------------------RTSSFTSLYSKP 493
             L    G   N      + + H++ P                      T++++ +Y   
Sbjct: 434 SQLRGAQGSTYNDGGGVATQYQHLDSPNYCLNNYGLNPAVASMMANQLGTNNYSPVYENA 493

Query: 494 NSSGFVSKDGPTRHLQNANL----QNVDLTGYTSGDFSINMNHSSAMNSYGASDHIKLPS 553
           +++  +   G    L         QN+  +       +  M   + + S+ A       +
Sbjct: 494 SAASAMGFSGMDSRLHGGGYVSSGQNLSESRNLGRFSNRMMGGGTGLQSHMADPMYHQYA 553

Query: 554 GTSDRANHAGSSLQPHNYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGNSSVYN--- 613
            + D  N     +   N+ G S  ++  L+ AYL A  +Q    Y+     + + Y    
Sbjct: 554 DSLDLLNDPSMDV---NFMGNSYMNMLELQRAYLGAQKSQYGVPYKSGSPNSHTDYGSPT 613

Query: 614 ----------HRLYTNT----------------------PYGSGIPYLADQALDSGLSSV 673
                     H L  N+                       Y  G+  +    +D+ L   
Sbjct: 614 FGSYPGSPLAHHLLPNSLVSPCSPMRRGEVNMRYPSATRNYAGGV--MGSWHMDASLDE- 673

Query: 674 GHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETANVEEKTKIFPEII 733
           G G ++L+  +  +      + I         DQYGSRFIQQKLETA  +EK  ++ EI+
Sbjct: 674 GFGSSMLEEFKSNKTRGFELAEIAGHVVEFSSDQYGSRFIQQKLETATSDEKNMVYEEIM 733

Query: 734 PHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMYGCRVIQKALEVVD 793
           PHA  LMTDVFGNYVIQKFFEHG   QR+ELAD+L  +VLPLSLQMYGCRVIQKA+EVVD
Sbjct: 734 PHALALMTDVFGNYVIQKFFEHGLPPQRRELADKLFDNVLPLSLQMYGCRVIQKAIEVVD 793

Query: 794 SDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAFYGQVLALSTHPYG 853
            DQ+ +MV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F+G V+ LSTHPYG
Sbjct: 794 LDQKIKMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGNVVTLSTHPYG 853

Query: 854 CRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEFGKPHERSAIISKL 913
           CRVIQRVLEHC D +TQ  +MDEIM ++ +L+QDQYGNYVIQHVLE GKP ER+ II +L
Sbjct: 854 CRVIQRVLEHCHDPDTQSKVMDEIMSTISMLAQDQYGNYVIQHVLEHGKPDERTVIIKEL 913

Query: 914 AGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQAMMKDPFGNYVVQ 939
           AG+IV+MSQQKFASNVVEKCLTFG PEER+ L+NE+LG+TDENEPLQAMMKD F NYVVQ
Sbjct: 914 AGKIVQMSQQKFASNVVEKCLTFGGPEEREFLVNEMLGTTDENEPLQAMMKDQFANYVVQ 957

BLAST of Lsi04G000820 vs. TAIR 10
Match: AT2G29140.1 (pumilio 3 )

HSP 1 Score: 631.3 bits (1627), Expect = 1.3e-180
Identity = 423/1018 (41.55%), Postives = 553/1018 (54.32%), Query Frame = 0

Query: 14  NEDHQVPLGNFEDNLRSELELLMRENSNHSVAG--RDGDLNIYRSGSAPPTVEGSLNAVG 73
           NED      +F D+   E+ +L+ E     V     + +LN+YRSGSAPPTV+GSL+A G
Sbjct: 15  NED-----SSFGDDYEKEIGVLLGEQQRRQVEADELERELNLYRSGSAPPTVDGSLSAAG 74

Query: 74  SLFTSSYYSEFNTKSGSNDG---VLSEDEIRSHPDYLSYYYSNDYINPRLPPPLVSKEDW 133
            LF+    + F    G N G      ++E R  P YLSYYY+N  +NPRLPPPL+S+ED 
Sbjct: 75  GLFSGGGGASFLEFGGVNKGNGFGGDDEEFRKDPAYLSYYYANMKLNPRLPPPLMSREDL 134

Query: 134 RVAQRFQGSGSSLGRQGDWNWKKLVDGNTSSSLFSMQPGSSVQRTDKNNSNVMEFGDANG 193
           RVAQR +GS + LG  GD   ++ V  N S SLFSM PG                    G
Sbjct: 135 RVAQRLKGSNNVLGGVGD---RRKV--NDSRSLFSMPPGFEA-----------------G 194

Query: 194 KNLPRKALSEWHDRGREGFVGSCGNGLGARRKSFADIVQEGLGESASLSGQLSRPASHNS 253
           K  P  + SEW   G  G       GLG ++KSFADI Q  +G    +  Q SRPAS N+
Sbjct: 195 K--PGASASEWDANGLIGL------GLGGKQKSFADIFQADMGH--PVVQQPSRPASRNT 254

Query: 254 FGDVDNMGMTDIKPPGLCNGLGPIEDLHNSGPPGFVGVQSHNKPVSHSFLNPNCSTLSRS 313
           F   +N+   +   P    G+G                     P  + +     S+LSR+
Sbjct: 255 FD--ENVDSNNNLSPSASQGIG--------------------APSPYCYAAVLGSSLSRN 314

Query: 314 TTPEPQLVGRSSSSGLPPVGS-RVFPVEKKNITVSKVQNGYSAGFTELPD----ISGLHL 373
            TP+PQ + R  S  L P+GS RV   +K+N +     NG ++G  E  D    +SGL+L
Sbjct: 315 GTPDPQGIARVPSPCLTPIGSGRVSSNDKRNTSNQSPFNGVTSGLNESSDLVNALSGLNL 374

Query: 374 SSIRPEDGVNGVQSRLQL--DLGEQSDFLINMSNG---IHTRTLPELSDKKLSNPS-DNI 433
           S      G  G+  R Q   D+ +  +++  + +G   ++    P  SD+     S  N 
Sbjct: 375 S------GTGGLDERGQAEQDVEKVRNYMFGLQDGHNEVNPHGFPNRSDQARGTASCRNS 434

Query: 434 DLARKSGIVVNPRASTMSSHDHVNFP-----------------------------KRTSS 493
            +    G   N  +   + + H + P                               T  
Sbjct: 435 QMRGSQGSAYNSGSGVANPYQHHDSPNYYALNPAVASMMANQLGTNNYSPMYENASATLG 494

Query: 494 FTSLYSKPNSSGFVSKDGPTRHLQN----ANLQNVDLTGYTSGDFSINMNHSSAMNSYGA 553
           ++++ S+ +   FVS        +N     N      TG+ S   +  M H  A  S  A
Sbjct: 495 YSAMDSRLHGGSFVSSGQNLSESRNIGRVGNRMMEGGTGHPS-HLADPMYHQYARFSENA 554

Query: 554 SDHIKLPSGTSDRANHAGSSLQPHNYYGISQGDLQGLRSAYLEALLAQQKQHYELSLSGN 613
                L   + DR             YG S  ++  ++ AY    L  QK  Y L    +
Sbjct: 555 DSFDLLNDPSMDR------------NYGNSYMNMLEIQRAY----LGSQKSQYGLPYK-S 614

Query: 614 SSVYNHRLYTNTPYGSGIPY----LADQALDSGLSS------------------------ 673
            S  +H  Y +  +GS + Y    LA   + + L S                        
Sbjct: 615 GSPNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRGEVNMRYPAATRNYTGG 674

Query: 674 ------------VGHGGTVLQNERILRFNSMMRSSIGAQ----GMDQYGSRFIQQKLETA 733
                        G G ++L+  +  +      S I         DQYGSRFIQQKLETA
Sbjct: 675 VMGSWHMDASLDEGFGSSMLEEFKSNKTRGFELSEIAGHVVEFSSDQYGSRFIQQKLETA 734

Query: 734 NVEEKTKIFPEIIPHARTLMTDVFGNYVIQKFFEHGTVTQRKELADQLSGHVLPLSLQMY 793
             +EK  ++ EI+P A  LMTDVFGNYVIQKFFEHG   QR+EL ++L  +VLPLSLQMY
Sbjct: 735 TTDEKNMVYEEIMPKALALMTDVFGNYVIQKFFEHGLPPQRRELGEKLIDNVLPLSLQMY 794

Query: 794 GCRVIQKALEVVDSDQQTQMVAELDGSVMKCVRDQNGNHVIQKCIECIPQDRIQFIISAF 853
           GCRVIQKA+EVVD DQ+ QMV ELDG VM+CVRDQNGNHV+QKCIEC+P++ I+FIIS F
Sbjct: 795 GCRVIQKAIEVVDLDQKIQMVKELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTF 854

Query: 854 YGQVLALSTHPYGCRVIQRVLEHCDDSNTQQIIMDEIMQSVCLLSQDQYGNYVIQHVLEF 913
           +G V+ LSTHPYGCRVIQRVLEHC + +TQ  +M+EI+ +V +L+QDQYGNYV+QHVLE 
Sbjct: 855 FGHVVTLSTHPYGCRVIQRVLEHCHNPDTQSKVMEEILSTVSMLTQDQYGNYVVQHVLEH 914

Query: 914 GKPHERSAIISKLAGQIVKMSQQKFASNVVEKCLTFGSPEERQLLINEILGSTDENEPLQ 939
           GKP ER+ II +LAG+IV+MSQQKFASNVVEKCLTFG PEER+LL+NE+LG+TDENEPLQ
Sbjct: 915 GKPDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFGGPEERELLVNEMLGTTDENEPLQ 949