Lsi04G000510 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G000510
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncrossover junction endonuclease MUS81
Locationchr04: 655575 .. 664576 (-)
RNA-Seq ExpressionLsi04G000510
SyntenyLsi04G000510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAATTCAAAATCGCGAAGCCCAGTTTACCGCGCGAGGCTGGGCTTCGTTTCCCTTTTCCACTAGTCGTTTCTTCACAATTCTCCAACAATTTACCATATTCCTCTTCTAAACCTCGAATTCTTGAAATGGACCGGCAAAGATCTCCGGTCGTCTGTCCGGAGAATGAAGACCTTGCCGCCTTCATGTGGCAGAAGCGGCTAGAAATGGCGGAGACACCCAAAGGAATCTCTGACAAAGTCGATATGACTCTATACAAAGCCTATTTCAACATCTGCAACTCCAAGGCCCCCATTAAGTCCATGAAGGATCTCTCCCAGATCAAGTAAGTTGTGTATCACCTCACTGTTTCGTATGTTTCGCTTGGCGATGGAATTGAATGATCGAATGCCAATGCTGTTTAGTTGTTTTCTGTTTTCGTTTGTTTGTGTCGAACTGAATCTGGAAGGTTTGGGTCATAACGAAGGTCTTTATTAGTTTCCTTAGAAGCTATAGTTTAAACGGGACTGTTCTCTGTGGTTCTTTGTTCTTTTAGTACTTCTCAATAATGTACTTTATTTTGTAGGAGGAATTAGATATGTAGGTGAAAGAGTTGCATTTTTCTTTTTCTCATTCTGCTTCTTAATTATCAGTATATGAAAGTAGTTACATAGTGTCTTTCATTCTTTGAATGCACTGTCTCATTAATATGGACTTCTGTCTTCTATCCTCTTTTTTGAAGTATTGATCTTTCTAATGCCGCAGAGGCGTAGGGAAGTGGATTCTTAAACAAATACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGGACCTGAGGAATTGGCGGGAAAAGGTACCTTAAACGAGCTCCGCGTTCTTTGTTTTTTAATGGGTTGTCATACAAGGAGACGTTTATTGCATTTCTTATTGGTTTCCTTTTTCCTTTTTATTCATCTAGGTAAGAAAACAAAGGGGACCAAACGCTATGTTCCACAGAAGAATTCAGTTGCATATGCCTTATTGATCACTCTTTATAGGTACAATGGACAAATTGTAATTTCTTTTGTCAAGTTCTTATTTAAATTTACGTGTAACATACGCTCCACTATTAGGGGTACAACAAATGGGAATGAATACATGCGTAAGCAGGAGCTTATTGATGCAGCTGAAGCAAGCGGTCTTTCTAGGGTGGCGATTGGGTAAGTTAGTTCCTGCATTCTTCTTTATGCTGTACGTTTTATCATTTAATGGATGTAGAGTAATTTTCCATTCTCAGGCCAGAGAAGGGTAAAGGAAAAGCTAGTCAATTTGGAAGCTCTCCTAGGGATTGGTACAGCGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAATTGCCCAGCAAAGTATGGTCTTGGCTTGGTCATAATTTTTCCCACCGTCAAATTTCCCCGCTCTTATTATTTTAGAATGATTTTCTGTGTCATCACATCATTATTTTCGTGGCTGGGGATGGGTACCATGTGATTGTTAAGGACTTGATACTGATGGCTGTAAGCTGCTCTAGTTTACATAGGAATAACGTACAAGTACCATCTGACCTAAGCAACATGTTAATATATGTGATGGCACATATTTATATTCTTAAGTGGTGAGTATTTTTTACTCTCGAACCTTTTTATATGTAGTATGTTCCTGCGAGAAGAAGAACACAAAGAAAATCGTGTTGAACCACCAATCGATGCAAAGGAGTATTAGGCAATATCAAGTAGTTTCTCTTGCATTGCCCACAGCATGTGGTAGCTTCAGCTTGCTTCATGGGCTATCCTGTTCTGATATATAGAGGATTCTTGTAGCTTTAGACGCTGGGCCTTGGAATAATGGCTCAGTACATAGATAAATATTTTGCAAGAATGGTATTGTAGAACACTTGAGTTATTTAGCATAGATTGTTAGCAGCCTTAAAGAATAAAAAATAATCCTGCAAAGCTTTAAGGGAACATATTATGAGGCTTAACTTGCATCAGAATACCGCATCAATTGTGCTTAAATCTGACACCTAAGATTTCTTGAATGTCATTAGTAATTTTATTCAAACGGTGACATCAACTTTTATGTGGAGTATTTCTTACAGAATTAAAACAATAAAACAACATTCTATACCCTGGTAATTTGTTTAAAACATTGTCTTTTTTTATAAGCCATGTTTGAATAATATTCTAGATCATGTCACTCTTCTTGTTCTATTATTTGCAAGTTATGCTAATATTTTTTGTTTCTACAGATACATGCTTACAGAGGAGGGGCAGCACGTGGCACGTGAATGTCTTATGAGATCTGGACTGTTAAGTTCTACAAAGAACTTTGATAACACAAAAGGATCCTCTATTCTTGAATCATGTGATATATCCGATCACAGTGCTAATTATCTTGATTCAGATGTAGAAGTGACTTCACCATCTCTTAGTAAGAGTAGTCAGAACAAATCAATCGATGTGCCCCTTGAATCACTTGAGAGGGTAATTCAAAGATTTAGATGTCACATTTTCATTAGTGAAATTGACTTGAAATGATTTATAACTTAAGCAATATGCAAATTGAGTATTCACAATTACACTCACGAATTGCCTGTGGCACTAGGGCTGCACACTAGATACAACTCTTTAACCATTGTTAGTTTGAACTCCATTTGTTCTTCACCTAATCCCCAAGCCCTAGCCAACTGGGCATCCCTTGTGGGCCATTCGCCATCTATTTCTGATTCTCTTTTTGTCTGTTTTTTCCCCTCCTTTTACAGTTTCTGCGCATGGGGTACTCTAAAAATCAAGTTCTTGATTCCTTTAAAGAAGTTTCAGAGGATAACCCGAACAAGGATATCTCATCACTTTGGCCAGCCGTTTTATGTCGTCTACGTGAAGATCTTGTTTATGGTTTGCTCTTAGAACTCTTAGTAGGAAATTAGCATATTGGGACAACATTACATTAGTTTCTAGAGAACTAACCCAAATATGGTTATGAGCTTTGTTATAAAAAACTGGGAAAAAATGGTTATAAGCTTTTTGATAGCATAAGCCTGTTTATTACCGCAGCCATGAAGGATATGGAGTTTTTGTTTTTGTTTTTGTTTTTTTTTAAATAAATCTTTTTAGGGTTGTTTTCAATTGTAGCAAAATGAGCCAGTTTATTTACAAATATAGCAAAATGTCATTATCCATCAGTGAAAAACACTGATGGATGTTCACTGATGGATGATAGTAGTCTATCACCGATAGAAAGTGACATTTTGCTATATTTGAAAATATTTACAGCAGTTTTTCTATTTAAAATAATTACCTTTGTATATATATATATATATATATATCGAAACGTACTGATCACTAGATTTATAAGTTGTATTTGTACAGGTCAAGTAGAAGCAGTTGGAAGAGAAAATAGGTCGATGGGTTCTTCTTCTCATATGGGGAATGGGCCAAAGATTGTTGTTAATGATGATGGAAAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTGTAAGTGGATATTCTAGCTTGCGCACACCCCCACACAACAACCGCAACAGCAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGTTTTGTATATAATAATTGTTTAAACGTAGTTTTGTATATAATAATTGTTTTTTGTTACTCCTTTAATTTTTTAGTATTTTATTTCCTTGTATAATTAGTTACTTTAGATGTTATTTCCTTGTATTTTATATTTACCCATTAGGTTGGACTTTTGAATTATAAGAAAAATTAATTCTCCAAAAACATCGAAAAAAGAAAAAAGAAAAAGAGAAAGAAAAAGAAAAAAAGAAAGAAAATAAAAGAGAGAAAAAAGGGTTCTTTATGCAAAGAAAGTACTTCATTACAAAATTGGTAATGCCTTCTTTCTGAAATGTATTTACTATTTACTATTATATTATGAAGCATTGAATCTTTATTGTACTTTGTATTTATAGAATCCTTCCATCACTGAATCCCGTAGAGATGGCGTCAAAGACAATATGAACATCTTGAGCATGCCACCTTTGAGCTTCGGGGAGAAATTTGAAGATTCTTATAACGTAGTCCTGATATTGGACGATCGAGAACAGTTTGCCAACCACAGGTCAGCAATTTTAATATACAATATACAATATAACATGTCTAGATGTGGGTTTTATTTTATAGGTTAAAACACATTTTTAGAAGTTCAAGGTTTGCACTTTTTGTTAACCTTTTGGAATAAACTACAGCCTGCAGTGCACCTTACTTCATTTTAGCTGTAACAAACAGTGAGCCTTTGTCTTCTTCATTAATCAATGAGAAGTTTGAACATTTCTGTTCCTTATAATTAGTTAACGAAATTTTGTATCTTGTTAAAAAAAAATAAAGCAATGCTTGTATCTTGGCTTGGATATTTTGTTTATACCCTCCTCTTTTTCACACGTAGCACAAGTTTCCTCTTAAAAAGCCGTATGCAATATATTTTGTAATTTTTATTTAGGAGCATTATTATTTTTGTGATACACGTGAAAGGAAAATCTACTAATCGAGGACTCGTGAAAACTCGTCTTCCCTGGCATGTCATGTGCATGCAAACTTGCAGTTGTTTGTTGAAATTTGACATTTTGCTATTCTACTTGTATTTTAATTACACTGCTAAGCTTTTATGTCATTGTGGTTTTCTAGTTCTCGGTCTAGGAGAATGATTGAGAATATCTGTAGCCAATTCAAGATCCAGATTGAGGTAACTTGGTTTTGTTCTTCTGTGAAAGTTTCTTTTGCATAATGCATTTCTATGTTATTAGGTTATCAATTAAATTTTATACTTATTTAAGAGTTTGCCTCCATAATCATTTTCAGCACGTTTGACCTACTTCTTGAATAGTAATGATATTATACCTGTAATGTATGTAACAATGGATATCCCCTATTCAACACGGAACATGTTGTCCACAGTGAGAAACAGCTTATTAAAGTGTGTTTTAGCAAATGCATGTCGCCACGTCTATTAATTCTTGTCAGCAGTCGGCTAGTGGCTAGTTCCATAATTAATCAATTACTTGGTTATAACCGACTCACACAATTTTTCTTCAGATTAGAAGGCTACCTGTTGGCGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAACGAATATGTTCTCGATTTTATTGTTGAGAGGAAAAATATTGATGATTTGCGGTGCTCAATCAGGGACAATCGCTATAGAGACCAGAAATTAAAACTTTTGGTAACTTTTTAACCAATTGATATTTTTCTATTTCCCTTCCGTGGTATCTGCATTCTGTCAATCTATGAAATGTCTTTTTCTCTCCTCTCCTGAATCAGTTGGTGAATTTCGATATTGATATGCTATGGACGAGAAATACAAATAGGCTTGTGGAACCTGTCACTACTTCCATTCCAAAACTGGGTTTTGTTATTAATATGTTTGTTCCCATTTCTATAGCTTTTAGATGGCAGCTCTTGCTCCGTCCTCAGTGTATCCCCGCTTCTTTACTTTCATTCTTGTCAATAAAATTTTTGTTCTTATTTAGTAAAAAAAAGAGAGAAATATAAACTGCAGCAGCAGCCTTGCTACTGTTATTTTTATATTGCCACCAAAGGATTTTCTGCTTGATTCTGTTTCAATTAGCCCAACTTTTCTGGGGACTACGAGGATGTATCATGTATTAGCTCATGTGAATATCAAAATTGATAATGCATTGTCGAAAATTGGTAGAAAATATTGGGCTAAGTGAGCTTAAATGCCAAAGTCCATTGGTTATTTATTATCATTTTGGCAGAATATTATGGGCAGATTACTGCAAGGGTGCAGATTCTCGCTTAGTTATTTGTCTTTGTGGCTAGCTTCTTACTAATTAATGTTTCTTGCTTTATCTCCTTAGAGATGTGGGCTTAAAAGGATGATATATCTTGTGGAAGGTGATCCCAATTCGTCTGAAGCGGCAGAAAGCATCAAAACAGCGTAAGTGTGGTTCTTCTTGATTATCATGCCAAGTATTAAACAAATTTGGTTCTTTGTTCCTAATGATTTTTTACGAGTATTATGATCATGAAATCTGAACTCCTTGTTGTATTAGTGGTTACAAAACGTGCTCTCTTAGTTAACAATGAGAAAAAAGGATGCCATGATCTTTAGAAATTAAAATAATGTGTCTGGATGATGTAGAAGTGGCTTGGGATTTTGATTCATGCGGGTAGATAATCGTGCAGTTGGTTCATGTTGTGTCTCTACAGAAATTTGTATTGGTCTGGTAGTCGGTCCTTTACTTTTATCTTTATCAATCCTCTCTACAAGTTCTTGTTGAACAGAACATTTAGTATGCACATTTACAATTATTATGTTGGTAGACCCCTAATCGTGTATGGTAGGAGGAAAGGGAGGGCTAGTGATCTCAAGCTATGAATGTGAGCGTGAGAGTCTGATACATGGGTGGGGCCCGCTTGAAATTACGTAAATATGGTTAAATACCGTAGTGCAAGTGGGCTCACGGCAAGAGTGTCATATTATACTGTACATTTAACTTTCATCAGTTTTTCAGGTTGTTTTGCATCTGATTCTCATCCAACCAAAAAAAAAAAATTGGTATGTTTTATGTTCTGCAGTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTACAAAGAACCAGTGGCTTGTCAGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACGTATTACAGGCATCTGATGAGCGTCTTAGTCATTCTGATGCCTGTCCTCGTCTCAATGAATTTATTAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTACAGCTCATGCAGGTATGAACATCTTTTATAAAGGAAAGCAAAGTTCATTCTCAGAAGTCTATGATCATTCAAAGCCTAATAGCATGTATGTATACAGGTCCCGCAGGTTACGGAGGAGGTTGCTGTTGCTGTTTTGGATTTGTACCCCACTCTTCTATCACTTGCCCGAGCATACTCTCTTTTAGTGAGTTGTTTTTTCTTTCTTCTATTTCTTCAGGTTAATTATTCTCCTTTTGATTTCATGCTTACTGGGAATTGGGATAGGTTTCAGACATTTGTGATAGGTGAAAGGAACAAAGTTCCCATCTTCATATATTTAATATTCTTCAAGTTAACCAACTTATCCCAATTCCTTTGGCATGGTAACCTTAACTTCAAAACCATATAATTGACAAAAAAACTGTCAATCTACTGAAACATAGGACTAAGAGTACTTCTTTTGGGCAAGTCATTTTTAGCGAGGCTATAGGTTACTTAATCACTTTGGGGGTGTTTGGAGCTCATGACTGCATAAACCTATGTTTTAGAAGTTTAGGCTTACGACAAATATTTCTAAGCTATTGCACCCACAAAATAAGTAAAAATTGTGTTAGTCCAAACTTCTTTAATTAATTCCTTATACAATAAAAATTCATATTATAAGAACTCAAGTTTTTGCTTGTAAGAGAAGTTTTTATTTGTCTATTTATTTCTTAAGTAATTTTCTTTTTTCTTTTTTCATTTTAAATGAAAAACTAGGACGGTAATGTTACTGAACAAGAAGAGATGTTAAGGAGACAGAGCAACAATTTGATCAATGCAGGTGCCAGCCGGAATATTTTCCATTTAGTATGGGGCTGTGGGAAGTAGAGTTCGCATATTCGCATATATCCGCTTTTCTCTTTACCTTGATGGCTGAGTTTTTTAGTCACCGCTGTAAATGTGCTTCTTATTTATTTTAAATGCCATGTTCATATGCTATAGTTTTCTCAGGGTTGATTGCGATTGTACAGTTTGTCTCTAGTTAGCGAAAAGTTGAAGAGTTATTGAGATGAAAGAAACTTGCATCAAGTTCTCTAGCTAGTAGCTTGTATTCACTGGGAGATTTTCATTATTGGGATAAAGTTGCATCTCCTCTCTAATAGAATTTTGGTTAATATATATAACGCCTTGAAATTTGCCCATGG

mRNA sequence

GGAAATTCAAAATCGCGAAGCCCAGTTTACCGCGCGAGGCTGGGCTTCGTTTCCCTTTTCCACTAGTCGTTTCTTCACAATTCTCCAACAATTTACCATATTCCTCTTCTAAACCTCGAATTCTTGAAATGGACCGGCAAAGATCTCCGGTCGTCTGTCCGGAGAATGAAGACCTTGCCGCCTTCATGTGGCAGAAGCGGCTAGAAATGGCGGAGACACCCAAAGGAATCTCTGACAAAGTCGATATGACTCTATACAAAGCCTATTTCAACATCTGCAACTCCAAGGCCCCCATTAAGTCCATGAAGGATCTCTCCCAGATCAAAGGCGTAGGGAAGTGGATTCTTAAACAAATACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGGACCTGAGGAATTGGCGGGAAAAGGTAAGAAAACAAAGGGGACCAAACGCTATGTTCCACAGAAGAATTCAGTTGCATATGCCTTATTGATCACTCTTTATAGGGGTACAACAAATGGGAATGAATACATGCGTAAGCAGGAGCTTATTGATGCAGCTGAAGCAAGCGGTCTTTCTAGGGTGGCGATTGGGCCAGAGAAGGGTAAAGGAAAAGCTAGTCAATTTGGAAGCTCTCCTAGGGATTGGTACAGCGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAATTGCCCAGCAAAATACATGCTTACAGAGGAGGGGCAGCACGTGGCACGTGAATGTCTTATGAGATCTGGACTGTTAAGTTCTACAAAGAACTTTGATAACACAAAAGGATCCTCTATTCTTGAATCATGTGATATATCCGATCACAGTGCTAATTATCTTGATTCAGATGTAGAAGTGACTTCACCATCTCTTAGTAAGAGTAGTCAGAACAAATCAATCGATGTGCCCCTTGAATCACTTGAGAGGTTTCTGCGCATGGGGTACTCTAAAAATCAAGTTCTTGATTCCTTTAAAGAAGTTTCAGAGGATAACCCGAACAAGGATATCTCATCACTTTGGCCAGCCGTTTTATGTCGTCTACGTGAAGATCTTGTTTATGGTCAAGTAGAAGCAGTTGGAAGAGAAAATAGGTCGATGGGTTCTTCTTCTCATATGGGGAATGGGCCAAAGATTGTTGTTAATGATGATGGAAAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTAATCCTTCCATCACTGAATCCCGTAGAGATGGCGTCAAAGACAATATGAACATCTTGAGCATGCCACCTTTGAGCTTCGGGGAGAAATTTGAAGATTCTTATAACGTAGTCCTGATATTGGACGATCGAGAACAGTTTGCCAACCACAGTTCTCGGTCTAGGAGAATGATTGAGAATATCTGTAGCCAATTCAAGATCCAGATTGAGATTAGAAGGCTACCTGTTGGCGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAACGAATATGTTCTCGATTTTATTGTTGAGAGGAAAAATATTGATGATTTGCGGTGCTCAATCAGGGACAATCGCTATAGAGACCAGAAATTAAAACTTTTGAGATGTGGGCTTAAAAGGATGATATATCTTGTGGAAGGTGATCCCAATTCGTCTGAAGCGGCAGAAAGCATCAAAACAGCTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTACAAAGAACCAGTGGCTTGTCAGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACGTATTACAGGCATCTGATGAGCGTCTTAGTCATTCTGATGCCTGTCCTCGTCTCAATGAATTTATTAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTACAGCTCATGCAGGTCCCGCAGGTTACGGAGGAGGTTGCTGTTGCTGTTTTGGATTTGTACCCCACTCTTCTATCACTTGCCCGAGCATACTCTCTTTTAGACGGTAATGTTACTGAACAAGAAGAGATGTTAAGGAGACAGAGCAACAATTTGATCAATGCAGGTGCCAGCCGGAATATTTTCCATTTAGTATGGGGCTGTGGGAAGTAGAGTTCGCATATTCGCATATATCCGCTTTTCTCTTTACCTTGATGGCTGAGTTTTTTAGTCACCGCTGTAAATGTGCTTCTTATTTATTTTAAATGCCATGTTCATATGCTATAGTTTTCTCAGGGTTGATTGCGATTGTACAGTTTGTCTCTAGTTAGCGAAAAGTTGAAGAGTTATTGAGATGAAAGAAACTTGCATCAAGTTCTCTAGCTAGTAGCTTGTATTCACTGGGAGATTTTCATTATTGGGATAAAGTTGCATCTCCTCTCTAATAGAATTTTGGTTAATATATATAACGCCTTGAAATTTGCCCATGG

Coding sequence (CDS)

ATGGACCGGCAAAGATCTCCGGTCGTCTGTCCGGAGAATGAAGACCTTGCCGCCTTCATGTGGCAGAAGCGGCTAGAAATGGCGGAGACACCCAAAGGAATCTCTGACAAAGTCGATATGACTCTATACAAAGCCTATTTCAACATCTGCAACTCCAAGGCCCCCATTAAGTCCATGAAGGATCTCTCCCAGATCAAAGGCGTAGGGAAGTGGATTCTTAAACAAATACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGGACCTGAGGAATTGGCGGGAAAAGGTAAGAAAACAAAGGGGACCAAACGCTATGTTCCACAGAAGAATTCAGTTGCATATGCCTTATTGATCACTCTTTATAGGGGTACAACAAATGGGAATGAATACATGCGTAAGCAGGAGCTTATTGATGCAGCTGAAGCAAGCGGTCTTTCTAGGGTGGCGATTGGGCCAGAGAAGGGTAAAGGAAAAGCTAGTCAATTTGGAAGCTCTCCTAGGGATTGGTACAGCGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAATTGCCCAGCAAAATACATGCTTACAGAGGAGGGGCAGCACGTGGCACGTGAATGTCTTATGAGATCTGGACTGTTAAGTTCTACAAAGAACTTTGATAACACAAAAGGATCCTCTATTCTTGAATCATGTGATATATCCGATCACAGTGCTAATTATCTTGATTCAGATGTAGAAGTGACTTCACCATCTCTTAGTAAGAGTAGTCAGAACAAATCAATCGATGTGCCCCTTGAATCACTTGAGAGGTTTCTGCGCATGGGGTACTCTAAAAATCAAGTTCTTGATTCCTTTAAAGAAGTTTCAGAGGATAACCCGAACAAGGATATCTCATCACTTTGGCCAGCCGTTTTATGTCGTCTACGTGAAGATCTTGTTTATGGTCAAGTAGAAGCAGTTGGAAGAGAAAATAGGTCGATGGGTTCTTCTTCTCATATGGGGAATGGGCCAAAGATTGTTGTTAATGATGATGGAAAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTAATCCTTCCATCACTGAATCCCGTAGAGATGGCGTCAAAGACAATATGAACATCTTGAGCATGCCACCTTTGAGCTTCGGGGAGAAATTTGAAGATTCTTATAACGTAGTCCTGATATTGGACGATCGAGAACAGTTTGCCAACCACAGTTCTCGGTCTAGGAGAATGATTGAGAATATCTGTAGCCAATTCAAGATCCAGATTGAGATTAGAAGGCTACCTGTTGGCGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAACGAATATGTTCTCGATTTTATTGTTGAGAGGAAAAATATTGATGATTTGCGGTGCTCAATCAGGGACAATCGCTATAGAGACCAGAAATTAAAACTTTTGAGATGTGGGCTTAAAAGGATGATATATCTTGTGGAAGGTGATCCCAATTCGTCTGAAGCGGCAGAAAGCATCAAAACAGCTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTACAAAGAACCAGTGGCTTGTCAGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACGTATTACAGGCATCTGATGAGCGTCTTAGTCATTCTGATGCCTGTCCTCGTCTCAATGAATTTATTAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTACAGCTCATGCAGGTCCCGCAGGTTACGGAGGAGGTTGCTGTTGCTGTTTTGGATTTGTACCCCACTCTTCTATCACTTGCCCGAGCATACTCTCTTTTAGACGGTAATGTTACTGAACAAGAAGAGATGTTAAGGAGACAGAGCAACAATTTGATCAATGCAGGTGCCAGCCGGAATATTTTCCATTTAGTATGGGGCTGTGGGAAGTAG

Protein sequence

MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITLYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK
Homology
BLAST of Lsi04G000510 vs. ExPASy Swiss-Prot
Match: Q5W9E7 (Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 2.6e-195
Identity = 365/668 (54.64%), Postives = 472/668 (70.66%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E AE PKG+S+ ++ T  K Y ++C++K PI ++K
Sbjct: 1   MDDERR-VLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGK--GKKTKGTKRYVPQKNSVAYALLI 120
           DLSQIKG GKW++K ++ +F +  E S  E+L     GKK  G KRY+PQ+NSV YALLI
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKT 180
           TL+R TTNG E+MRKQELIDAA+A+GLS   +GPEKGKGKA   G S R+WYSGWSCM T
Sbjct: 121 TLHRRTTNGKEFMRKQELIDAADANGLSHAPVGPEKGKGKAG-LGHSKREWYSGWSCMTT 180

Query: 181 LISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTK--NFDNTKGSSILESCDISD 240
           LI +GLVVKS+ PAKYMLT EG+ VA EC++RSGL  S    + D    +   +     +
Sbjct: 181 LIQKGLVVKSSNPAKYMLTVEGREVANECILRSGLPDSVDILSVDEMDPTPQAKKTPNQN 240

Query: 241 HSANY-LDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPN 300
            + ++ +  ++    P     S      +P + LE+F   GYSK QV+ +F+EVS+ + +
Sbjct: 241 PTCSFTMREELPYVDPRCRAQSA-----IPSDILEKFTPFGYSKEQVVAAFREVSDGSGD 300

Query: 301 KDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRAC 360
           KD S+LW +V+C LR+  VY    +      S   SS         V+ +G R+   R+C
Sbjct: 301 KDPSTLWLSVMCHLRQAEVY---NSCPDSRNSKKDSSGPFKSQIRQVDLEGSRAKKFRSC 360

Query: 361 ---SSSNPSITESRR-----------DGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDR 420
              S+ NP  + S             DG K   NI  +PPL FGE FE++Y+V+LILDDR
Sbjct: 361 NDGSTLNPCSSGSSHAVKACSSSLASDGTKGITNIPRLPPLQFGETFEEAYDVILILDDR 420

Query: 421 EQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNID 480
           E+FA   SRSR ++ENICS+F I+IE+RRLPVGD IWIARHK+L+ EYVLDFI ERKN+D
Sbjct: 421 EKFATKGSRSRNIVENICSEFNIKIEVRRLPVGDCIWIARHKYLETEYVLDFIAERKNVD 480

Query: 481 DLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQR 540
           D+R SIRDNRYRDQKL+L R G K++IY++EGDPN S+AAESIKTACFTTEILEGFDV R
Sbjct: 481 DMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDPNHSDAAESIKTACFTTEILEGFDVLR 540

Query: 541 TSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDA-CPRLNEFIKKCQELDKMTVSDVF 600
           T GL +TL+KYGYLT++I QYY L+ +D   S   A CP  + F+K+CQ+LDKMT+SDVF
Sbjct: 541 THGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKGAASCPSFDSFVKRCQDLDKMTISDVF 600

Query: 601 ALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASR 649
           A+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS L+ +V+ QEEMLR +SNN+I A AS+
Sbjct: 601 AIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSHLEADVSAQEEMLRNRSNNVICASASK 658

BLAST of Lsi04G000510 vs. ExPASy Swiss-Prot
Match: Q8GT06 (Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 9.1e-156
Identity = 327/666 (49.10%), Postives = 425/666 (63.81%), Query Frame = 0

Query: 12  ENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKW 71
           ENE +A  + +K   M E P G+ + +  TLYK+Y N+C +K PI+S+KDL QIKGVGKW
Sbjct: 14  ENEAVAQCVLEKWRSMEEKPGGLKENLAHTLYKSYRNVCAAKEPIRSLKDLYQIKGVGKW 73

Query: 72  ILKQIQDFFKSGSEGSGPEE--LAG-KGKKTKGTKRYVPQKNSVAYALLITLYRGTTNGN 131
           +++Q++  F   S    P E   AG KGKK  G+KRYVPQKNS AYA+LITL+R T NG 
Sbjct: 74  VIRQLKGSFPESSPDLSPPESNAAGEKGKKAGGSKRYVPQKNSAAYAILITLHRETINGK 133

Query: 132 EYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKS 191
            +M+KQELIDA EASGLSR AIGP+K K K   F SS +DWY+GWSCMKTL S+GLV KS
Sbjct: 134 SHMKKQELIDATEASGLSRSAIGPDKSKAKPGAFASSQKDWYTGWSCMKTLTSKGLVAKS 193

Query: 192 NCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSDVEV 251
             PAKYM+TEEG+  A ECL RSGL       D+     ++ S   + ++++ L +++ +
Sbjct: 194 GNPAKYMITEEGKSTALECLSRSGL-------DDHAAPLVINSAPDTSNASHKL-NNICM 253

Query: 252 TSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCR 311
           TS   + S  +++I  P  S+         +   L S + +   N N D+ +   A  C 
Sbjct: 254 TSFVETSSGPSRAIGRPKTSIANPATKTSPEVTYLTSQESL---NYNSDVRT---AENCA 313

Query: 312 LREDLVYGQVEAVGRENRSMGSSSHMGN---GPKIVVNDDGKRSFNLR--ACSSS-NPSI 371
               L     E +  EN  +  S         P +  ++ GK + N R   CS+S +P  
Sbjct: 314 EEIILSDSDSEELYTENYPLIGSEEFTERVAPPILNASNSGKTTTNYRFSDCSASISPRS 373

Query: 372 TESR------------------RDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQF 431
           +E                     D V  + +IL+MPP    + F + Y VVLILDDRE F
Sbjct: 374 SEGTFEMQSSSTMGIAEFNMLDNDTVCMDNSILAMPPRRSSKNFLEDYEVVLILDDRENF 433

Query: 432 ANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLR 491
                RSR+ ++NI SQF++ +EI+ LPVGDGIWIAR + L  EYVLDFIVERKN+ DL 
Sbjct: 434 ---GGRSRKTVDNIHSQFRVPVEIKHLPVGDGIWIARDRKLHTEYVLDFIVERKNVADLC 493

Query: 492 CSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSG 551
            SI DNRY+DQKL+L +CGL+++IYLVEGDPN  + +E IKTACFTTEILEGFDVQRT G
Sbjct: 494 SSITDNRYKDQKLRLKKCGLRKLIYLVEGDPNPLDTSERIKTACFTTEILEGFDVQRTPG 553

Query: 552 LSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQL 611
            ++T++ YG LT +IT+YY    S    + S  C   +EF KKC +L K+TVSDVFALQL
Sbjct: 554 YAETVRTYGNLTHSITEYYSTHFS-TGANTSQVCLTYDEFTKKCDDLKKITVSDVFALQL 613

Query: 612 MQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFH 651
           MQVPQVTEE A+AV+ LYPTL SLA+AYS+LDG+   QE+ML+ +S  LINAGASRNIF 
Sbjct: 614 MQVPQVTEEAALAVIGLYPTLFSLAKAYSMLDGDTHAQEKMLKNKS-TLINAGASRNIFK 660

BLAST of Lsi04G000510 vs. ExPASy Swiss-Prot
Match: Q6BJ48 (Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=MUS81 PE=3 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 4.7e-35
Identity = 181/713 (25.39%), Postives = 296/713 (41.51%), Query Frame = 0

Query: 21  WQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGK----WILKQI 80
           W +   +  T KG   K  +   KA  ++ N   PI   K L  ++ VG      + K++
Sbjct: 15  WLQEAAINATKKG--TKAAILYNKALGSVRNYPLPINDPKTLKSVQFVGDKTCIHLSKKL 74

Query: 81  QDFFKSGS---------------------EGSGPEELA-GKGKKTKGTKRYVPQKNSVAY 140
           +++ K  +                     E S    L   K KK +  K+Y+P+K S  Y
Sbjct: 75  EEYCKLNNFELPVAFGGLINGGVGEKRKHEVSDTSNLPDAKPKKQRKQKQYIPRKRSGGY 134

Query: 141 ALLITLYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWS 200
           A+L+ LY G       + K+E+I  A                 K+ +   S  ++YS WS
Sbjct: 135 AILLALYFGDKK-KTGLTKEEIIQRATPY------------SDKSFKSNPSANEFYSAWS 194

Query: 201 CMKTLISRGLVVKSNCPAK-YMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCD 260
            +K+L +  LV  S   +K Y LTEEG  +A++                 K +   ES  
Sbjct: 195 SIKSLQTHDLVDSSGRSSKSYFLTEEGYELAKQ----------------LKDAEGFESSP 254

Query: 261 ISDHSANY-LDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSK-NQVLDSFKEVSE 320
           I++H A+   D+ V VT  S                        YSK +Q LDS   +  
Sbjct: 255 ITNHIADLSFDNQVRVTPDS-----------------------SYSKISQQLDSSPLMKT 314

Query: 321 DNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFN 380
            N NKD   L P+ L   RE ++                   + + P+++ +    + F 
Sbjct: 315 KN-NKDRYGLGPSRLSSTRERIL------------------DLSSSPRLISSPLKPKDFV 374

Query: 381 LR-ACSSSNPSITESRRDGV---KDNMNILSMPPLSF--------GEKFE----DSYNVV 440
           LR + SS N +     R+ +   +D +N  +   L          G  ++      + ++
Sbjct: 375 LRESLSSHNSTRPLQDRESISKAEDGINTKTKARLVHDASKRIYDGTNYDIWVPGEFEII 434

Query: 441 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 500
           LI+D+RE     S R R   +   +  K++ ++R L VGD +W A+HK    E +L++I 
Sbjct: 435 LIIDNRE---IRSQRDRDFFQTRLTSLKVECDVRPLSVGDVVWTAKHKKTGREVILNYIC 494

Query: 501 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVE----GDPNS-SEAAESIKTACFT 560
           ERK +DDL  SI+D R+++QK +L + G+K+  YLVE     D N   + +++I+TA   
Sbjct: 495 ERKRLDDLVSSIKDGRFQEQKNRLKKSGMKQFYYLVEDVVTSDMNKFGDMSDAIQTAMSM 554

Query: 561 TEILEGFDVQRTSGLSDTLKKYGYLTRAI--------TQYYVLQASD------------- 620
           T  +  F ++R   + DT+     LT+ I        T   VL+A               
Sbjct: 555 TMTISNFYLKRFKSIEDTIAFLASLTQVIKDQFAKNKTNLLVLKARSIKNQAEYSSLIAK 614

Query: 621 -----ERLSHSDACPRL-NEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYP 650
                E  S S  C  L + F     +   MTV + F L LM +  V+ E AVA+ + + 
Sbjct: 615 FKEKFENRSTSYECAHLFSTFQDSMGKTGMMTVKETFILMLMGIRGVSLERAVAIQNRFK 650

BLAST of Lsi04G000510 vs. ExPASy Swiss-Prot
Match: Q6CPQ8 (Crossover junction endonuclease MUS81 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=MUS81 PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.6e-33
Identity = 160/687 (23.29%), Postives = 270/687 (39.30%), Query Frame = 0

Query: 26  EMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKWILKQIQDFFKSGSE 85
           E+ ++     +K+ M L +A F + N   PI   KDL ++KG+G+  + ++    K+  E
Sbjct: 18  EVNQSTSAHQEKLVMVLNRALFALKNYPDPIYHPKDLLKVKGIGQTTMNKLSKRLKTYCE 77

Query: 86  GSG----------------------------------PEELAGKGKKTKGTKRYVPQKNS 145
            +G                                  PEE   +G + +  K+Y+P+  S
Sbjct: 78  ENGYSFPEESSSTDNTQSTQNDSNPSNTQKTRVRTLEPEENESQGTRKRRKKKYIPRNRS 137

Query: 146 VAYALLITLYR-GTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWY 205
             Y +L+ L   G         +++LI  A             K   ++ +   S +++Y
Sbjct: 138 GGYGILLGLLELGCDKDGTACTRRQLIAVA------------SKYCDQSYEKNPSTKEFY 197

Query: 206 SGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILE 265
           S WS +K+L +  LV++   P+++ LTE G  +A                D+ K      
Sbjct: 198 SAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILA----------------DSLK------ 257

Query: 266 SCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVS 325
                  +AN ++ DV                       ER L  G ++N   +      
Sbjct: 258 -------TANNIEFDVS-------------------SVYERRLNRGNTQNSFTN-----D 317

Query: 326 EDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSF 385
           E +   + S L       + E             N S        N  +I  N++ + S 
Sbjct: 318 EHDHTVNFSGLMNHANMSINES-----------ANSSRLFLDATANSSRIEQNEEPEVSA 377

Query: 386 NLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSR 445
            + +       +++ R  GVK  +      P        DSY+++L +D RE     S  
Sbjct: 378 EI-STPIPKQKVSKGRWKGVKYEL----WKP--------DSYDIILHIDHREV---RSKE 437

Query: 446 SRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDN 505
            R        Q  I+ E   L VGD IW+A+HK    +  LDFIVERK +DDL  SIRDN
Sbjct: 438 DRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSGQQCALDFIVERKRLDDLVISIRDN 497

Query: 506 RYRDQKLKLLRCGLKRMIYLVEGDP--NSSEAAESIKTACFTTEILEGFDVQRTSGLSDT 565
           R+ +QK +L + G K + YLVE     N S++A+ +KT+ +TT I   F ++RT     T
Sbjct: 498 RFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMMKTSIWTTVIYNDFHIKRTRNADTT 557

Query: 566 LK---------------------KYGYLTRA---ITQYYVLQASDERLSHSDACPRLNEF 625
           ++                      + ++T     +T   + +   ER    + C      
Sbjct: 558 VQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTSLKMFRTEFERNKEIECCHNYESM 608

Query: 626 IKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAY----SLLDGNVT 648
                + + MTV +++   LM V  ++ E A+ +   YPT  +L +AY    +  D    
Sbjct: 618 QSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSRYPTFKTLLKAYRRCAAEADAKTL 608

BLAST of Lsi04G000510 vs. ExPASy Swiss-Prot
Match: Q7SXA9 (Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 2.4e-31
Identity = 180/693 (25.97%), Postives = 283/693 (40.84%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           + R+R    CP    L    W   L  +   KG+  K      KA  ++     P+K+ K
Sbjct: 8   LGRKRPLPSCPNPLFL---QWLTELRDSAKEKGL--KTHFVYQKAINSLKKYPLPLKNGK 67

Query: 61  DLSQIKGVGKWILKQIQD-----FFKSGSEGS-----------GPEELAG---------- 120
           +   ++  G  I K + +     + ++GS+ +             +E +G          
Sbjct: 68  EAKILQNFGDGICKILDERLQKHYRENGSDAAVHLASSKQMEESQKEPSGNFSEHTKLTQ 127

Query: 121 -KGKKTKGTKR---YVPQKNSVAYALLITLYR--GTTNGNEYMRKQELIDAAEASGLSRV 180
            + +K KG K+   YVPQK S  YA+L+TLYR         +M + EL    EA  L   
Sbjct: 128 KEVRKEKGAKKKREYVPQKRSGGYAVLLTLYRHMQMPGSKGFMFRNEL--QTEAQPLCEK 187

Query: 181 AIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECL 240
           +             GS     Y+ WS + TLI + LVVK++ PA+Y LT++         
Sbjct: 188 SF-------TVPDLGSK----YTAWSSVSTLIQKELVVKTHNPARYSLTDQ--------- 247

Query: 241 MRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLES 300
                           G S+ E  D  +    + D D        S+  QN  +D+ LE 
Sbjct: 248 ----------------GLSLAEKLDSEETGTRHEDVD--------SQDGQN-VVDLTLE- 307

Query: 301 LERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSM 360
                              E  ED   +  SS  PAV       L   Q   +  E+  M
Sbjct: 308 -------------------EEDEDEEKESWSSERPAVA------LPVNQARGLMSESDPM 367

Query: 361 GSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKF 420
           G S           ++ G+ +                                LS G   
Sbjct: 368 GKSQ---------TSETGRTAMGWH----------------------------LSPG--- 427

Query: 421 EDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHK----- 480
             SY++VL +D  E     S R + +++ +  +  +  ++R+L VGD +W+AR +     
Sbjct: 428 --SYDIVLCVDLCETTGGSSVRKQELVKEL-QRNSVTFDVRKLNVGDFLWVARERVTPVP 487

Query: 481 -----HLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVE----GD 540
                 +  E VLD+I+ERK +DDL  SI D R+R+QK +L RCGL++ IYLVE      
Sbjct: 488 GQLRPPVGKELVLDYIIERKRMDDLCGSIIDGRFREQKFRLKRCGLRKPIYLVEECGSAA 547

Query: 541 PNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVL--------- 600
            + S    +++ A   T++++GF V+R     ++      +TR + + Y           
Sbjct: 548 AHLSIPESTLQQAIVNTQVVDGFFVKRVQDAKESAAYLTIMTRYLQKLYQNCTLLCRSRE 573

Query: 601 -----QASDERLSHSDACPRL--NEF----IK-KCQELDKMTVSDVFALQLMQVPQVTEE 627
                +A  E++  + +C  +   EF    IK KCQ     TV +VFA QLMQ+  V+ +
Sbjct: 608 LEGDGEAESEKMVANLSCSLMAFTEFNYGAIKNKCQ-----TVREVFARQLMQISGVSGD 573

BLAST of Lsi04G000510 vs. ExPASy TrEMBL
Match: A0A1S3BFN6 (crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489485 PE=3 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 599/651 (92.01%), Postives = 627/651 (96.31%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK SQFGSSPRDWYSGWSCMKTLI
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPSQFGSSPRDWYSGWSCMKTLI 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSS+KNFDN KGSSILESCDIS+HSAN
Sbjct: 181 SRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSSKNFDNIKGSSILESCDISNHSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           YLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RMGYSK+QV++SFKEVS+DNPNKD SS
Sbjct: 241 YLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRMGYSKHQVINSFKEVSKDNPNKDTSS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G+ PK VVND+G+RSFN RACSSSNP
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEGSEPKNVVNDNGERSFNSRACSSSNP 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SI ESRRD VK++ NILSMPPLSFGEKFED YNVVLILDDREQFA HSSRSRRMIENICS
Sbjct: 361 SIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVLILDDREQFAKHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVERK+IDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVERKDIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASD+R+SHSD CPRL++FIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD
Sbjct: 541 QYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG G+
Sbjct: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGSGR 649

BLAST of Lsi04G000510 vs. ExPASy TrEMBL
Match: A0A0A0KWV2 (ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 SV=1)

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 592/652 (90.80%), Postives = 623/652 (95.55%), Query Frame = 0

Query: 1   MDRQRSPVV-CPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSM 60
           MDRQRSP V CPENEDLA FMWQKRLEMA TPKGISDKVDMTLYKAY N+CNSK  I SM
Sbjct: 1   MDRQRSPAVFCPENEDLAEFMWQKRLEMAATPKGISDKVDMTLYKAYLNVCNSKVSITSM 60

Query: 61  KDLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLIT 120
           KDLSQIKGVGKWILKQ+QDFFKSGS+GS PEELAGKGKKTKGT+RYVPQKNSVAYALLIT
Sbjct: 61  KDLSQIKGVGKWILKQVQDFFKSGSDGSEPEELAGKGKKTKGTRRYVPQKNSVAYALLIT 120

Query: 121 LYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTL 180
           LYRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK SQFGSS RDWYSGWSCMKTL
Sbjct: 121 LYRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPSQFGSSSRDWYSGWSCMKTL 180

Query: 181 ISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSA 240
           ISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSS+KNFDNTKGSSILESCDIS+HSA
Sbjct: 181 ISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSSKNFDNTKGSSILESCDISNHSA 240

Query: 241 NYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDIS 300
           NYLDSDVEVTSPS+S+S+++KSIDVPLESLERF+RMGYSK+QV++SFKEVS+DNPNKDIS
Sbjct: 241 NYLDSDVEVTSPSVSQSTKHKSIDVPLESLERFMRMGYSKHQVINSFKEVSKDNPNKDIS 300

Query: 301 SLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSN 360
           SLWPAVLCRLREDLVYGQVEA+GRENRSMGSS   G+ PK VVN +G+RSF+ RACSSSN
Sbjct: 301 SLWPAVLCRLREDLVYGQVEAIGRENRSMGSSLE-GSEPKNVVNVNGERSFSSRACSSSN 360

Query: 361 PSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENIC 420
           PSI ESRRD VK N NILSMPPLSFGEKFED YNVVLILDDREQFA HSSRSRRMIENIC
Sbjct: 361 PSIIESRRDSVKGNTNILSMPPLSFGEKFEDCYNVVLILDDREQFAKHSSRSRRMIENIC 420

Query: 421 SQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKL 480
           SQFKIQIEIRRLPVGDGIW+ARHKHLDNEYVLDFIVERK+++DLRCSIRDNRYRDQKLKL
Sbjct: 421 SQFKIQIEIRRLPVGDGIWVARHKHLDNEYVLDFIVERKDVEDLRCSIRDNRYRDQKLKL 480

Query: 481 LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAI 540
           LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AI
Sbjct: 481 LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAI 540

Query: 541 TQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL 600
           TQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL
Sbjct: 541 TQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL 600

Query: 601 DLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           DLYPTLLSL RAYSLLDGNVTEQEEMLRR+SNNLINAGASRNIFHLVWGCGK
Sbjct: 601 DLYPTLLSLVRAYSLLDGNVTEQEEMLRRRSNNLINAGASRNIFHLVWGCGK 651

BLAST of Lsi04G000510 vs. ExPASy TrEMBL
Match: A0A6J1GYZ8 (crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458164 PE=3 SV=1)

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 594/651 (91.24%), Postives = 621/651 (95.39%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q  FK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK  QFGSS RDWYSGW+CMKTL+
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPGQFGSSTRDWYSGWNCMKTLV 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+S KNFD  KGSSILESC ISD SAN
Sbjct: 181 SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLNSEKNFDTMKGSSILESCGISDRSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           +LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RMGYSK+QVLDSFKEVSEDNPNKDISS
Sbjct: 241 HLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRMGYSKHQVLDSFKEVSEDNPNKDISS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRS GSS+  G+ PKIVV+D+G+RSFNLRACSSSN 
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSTGSSNE-GHVPKIVVSDNGERSFNLRACSSSNH 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SITESR+D VK+N NILSMPPLSFGEKFED+Y+VVLILDDREQFANHSSRSRRMIENICS
Sbjct: 361 SITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVLILDDREQFANHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASDERLSH D CPRL+ FIKKCQELDKMTVSDVFALQLMQVPQVTEE+AVAVLD
Sbjct: 541 QYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTVSDVFALQLMQVPQVTEEIAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA ASRNIFHLVWG GK
Sbjct: 601 LYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINAAASRNIFHLVWGSGK 650

BLAST of Lsi04G000510 vs. ExPASy TrEMBL
Match: A0A5A7SUV8 (Crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002880 PE=3 SV=1)

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 592/651 (90.94%), Postives = 619/651 (95.08%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK SQFGSSPRDWYSGWSCMKTLI
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPSQFGSSPRDWYSGWSCMKTLI 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSS+KNFDN KGSSILESCDIS+HSAN
Sbjct: 181 SRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSSKNFDNIKGSSILESCDISNHSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           YLDSDVEVTSPS+S+ ++NK+IDVPLESLER        +QV++SFKEVS+DNPNKD SS
Sbjct: 241 YLDSDVEVTSPSVSQGTKNKTIDVPLESLER--------HQVINSFKEVSKDNPNKDTSS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G+ PK VVND+G+RSFN RACSSSNP
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEGSEPKNVVNDNGERSFNSRACSSSNP 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SI ESRRD VK++ NILSMPPLSFGEKFED YNVVLILDDREQFA HSSRSRRMIENICS
Sbjct: 361 SIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVLILDDREQFAKHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVERK+IDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVERKDIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASD+R+SHSD CPRL++FIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD
Sbjct: 541 QYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG G+
Sbjct: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGSGR 641

BLAST of Lsi04G000510 vs. ExPASy TrEMBL
Match: A0A6J1JV85 (crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC111488639 PE=3 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 591/651 (90.78%), Postives = 617/651 (94.78%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKG SDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q FFKSGSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK  QFGSS R+WYSGW+CMKTL+
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPGQFGSSTREWYSGWNCMKTLV 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+S KNFD  KGSSILESCDIS  SAN
Sbjct: 181 SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLNSEKNFDTMKGSSILESCDISGRSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           +LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RMGYSK+QVLDSFKEVSEDNPNKDISS
Sbjct: 241 HLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRMGYSKHQVLDSFKEVSEDNPNKDISS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENR  GSS+  G+ PKIV++D+G+RSFNLRACSSSN 
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRLTGSSNE-GHVPKIVISDNGERSFNLRACSSSNQ 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SITES +D VK+NMNILSMPPLSFGEKFED+Y+VVLILDDREQFANHSSRSRRMIENICS
Sbjct: 361 SITESCKDVVKENMNILSMPPLSFGEKFEDAYSVVLILDDREQFANHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASDER SH D CPRL+ FIKKCQELDKMTVSDVFALQLMQVPQVTEE+AV VLD
Sbjct: 541 QYYILQASDERPSHFDVCPRLDAFIKKCQELDKMTVSDVFALQLMQVPQVTEEIAVTVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLD NVT QEEMLRRQSNNLINA ASRNIFHLVWG GK
Sbjct: 601 LYPTLLSLARAYSLLDDNVTHQEEMLRRQSNNLINAAASRNIFHLVWGSGK 650

BLAST of Lsi04G000510 vs. NCBI nr
Match: XP_038892703.1 (crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida])

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 616/653 (94.33%), Postives = 630/653 (96.48%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPVVCPENED+A FMWQKRLEMA TPKGISDKVDMTLYKAYFN+CNSK PIKSMK
Sbjct: 1   MDRQRSPVVCPENEDIATFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKVPIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+ DFFK+GSEGSGPEEL GKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVHDFFKNGSEGSGPEELVGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDA+EASGLSRVAI PEKGKGK SQFGSSPRDWYSGWSCMKTLI
Sbjct: 121 YRGTTNGNEYMRKQELIDASEASGLSRVAIAPEKGKGKPSQFGSSPRDWYSGWSCMKTLI 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSSTKN DNTKGSSILESCDISDHSAN
Sbjct: 181 SRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSTKNVDNTKGSSILESCDISDHSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
            LDSDVEVTSP+  KSSQNKS DVPLESLERF+RMGYSK+QVLDSFKEVSEDNPNKDISS
Sbjct: 241 SLDSDVEVTSPTAIKSSQNKSTDVPLESLERFMRMGYSKHQVLDSFKEVSEDNPNKDISS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENR-SMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSN 360
           LWPAVLCRLREDLVYGQVEAVGRENR +  SSSH  NGPKIVVND+G+R FNLRACSSSN
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRLTSSSSSHDRNGPKIVVNDNGERFFNLRACSSSN 360

Query: 361 PSI-TESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENI 420
           PSI +ES RDGVK NMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENI
Sbjct: 361 PSISSESCRDGVKANMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENI 420

Query: 421 CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLK 480
           CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLK
Sbjct: 421 CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLK 480

Query: 481 LLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRA 540
           LLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRA
Sbjct: 481 LLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRA 540

Query: 541 ITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAV 600
           ITQYY+LQASDERLSHSD CPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAV
Sbjct: 541 ITQYYILQASDERLSHSDVCPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAV 600

Query: 601 LDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           L+LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK
Sbjct: 601 LELYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 653

BLAST of Lsi04G000510 vs. NCBI nr
Match: XP_008446914.1 (PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_008446915.1 PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo])

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 599/651 (92.01%), Postives = 627/651 (96.31%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK SQFGSSPRDWYSGWSCMKTLI
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPSQFGSSPRDWYSGWSCMKTLI 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSS+KNFDN KGSSILESCDIS+HSAN
Sbjct: 181 SRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSSKNFDNIKGSSILESCDISNHSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           YLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RMGYSK+QV++SFKEVS+DNPNKD SS
Sbjct: 241 YLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRMGYSKHQVINSFKEVSKDNPNKDTSS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G+ PK VVND+G+RSFN RACSSSNP
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEGSEPKNVVNDNGERSFNSRACSSSNP 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SI ESRRD VK++ NILSMPPLSFGEKFED YNVVLILDDREQFA HSSRSRRMIENICS
Sbjct: 361 SIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVLILDDREQFAKHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVERK+IDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVERKDIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASD+R+SHSD CPRL++FIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD
Sbjct: 541 QYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG G+
Sbjct: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGSGR 649

BLAST of Lsi04G000510 vs. NCBI nr
Match: XP_023514312.1 (crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 595/651 (91.40%), Postives = 622/651 (95.55%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q FFK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK  QFGSS RDWYSGW+CMKTL+
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPGQFGSSTRDWYSGWNCMKTLV 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+S KNFD  KGSSILESCDISD SAN
Sbjct: 181 SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLNSEKNFDTMKGSSILESCDISDRSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           +LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RMGYSK+QVLDSFKEVSEDNPNKDISS
Sbjct: 241 HLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRMGYSKHQVLDSFKEVSEDNPNKDISS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRS GS+   G+ PKIVV+D+G+RSFNLRACSSSN 
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSTGSNE--GHVPKIVVSDNGERSFNLRACSSSNH 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SITESR+D VK+N NILSMPPLSFGEKFED+Y++VLILDDREQFANHSSRSRRMIENICS
Sbjct: 361 SITESRKDVVKENTNILSMPPLSFGEKFEDAYSIVLILDDREQFANHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASDERLSH D CPRL+ FIKKCQELDKMTVSDVFALQLMQVPQVTEE+AVAVLD
Sbjct: 541 QYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTVSDVFALQLMQVPQVTEEIAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA ASRNIFHLVWG GK
Sbjct: 601 LYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINAAASRNIFHLVWGSGK 649

BLAST of Lsi04G000510 vs. NCBI nr
Match: XP_022956424.1 (crossover junction endonuclease MUS81 [Cucurbita moschata])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 594/651 (91.24%), Postives = 621/651 (95.39%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q  FK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLI 180
           YRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK  QFGSS RDWYSGW+CMKTL+
Sbjct: 121 YRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPGQFGSSTRDWYSGWNCMKTLV 180

Query: 181 SRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSAN 240
           SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+S KNFD  KGSSILESC ISD SAN
Sbjct: 181 SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLNSEKNFDTMKGSSILESCGISDRSAN 240

Query: 241 YLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISS 300
           +LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RMGYSK+QVLDSFKEVSEDNPNKDISS
Sbjct: 241 HLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRMGYSKHQVLDSFKEVSEDNPNKDISS 300

Query: 301 LWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNP 360
           LWPAVLCRLREDLVYGQVEAVGRENRS GSS+  G+ PKIVV+D+G+RSFNLRACSSSN 
Sbjct: 301 LWPAVLCRLREDLVYGQVEAVGRENRSTGSSNE-GHVPKIVVSDNGERSFNLRACSSSNH 360

Query: 361 SITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICS 420
           SITESR+D VK+N NILSMPPLSFGEKFED+Y+VVLILDDREQFANHSSRSRRMIENICS
Sbjct: 361 SITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVLILDDREQFANHSSRSRRMIENICS 420

Query: 421 QFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480
           QFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL
Sbjct: 421 QFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLL 480

Query: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAIT 540
           RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AIT
Sbjct: 481 RCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAIT 540

Query: 541 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 600
           QYY+LQASDERLSH D CPRL+ FIKKCQELDKMTVSDVFALQLMQVPQVTEE+AVAVLD
Sbjct: 541 QYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTVSDVFALQLMQVPQVTEEIAVAVLD 600

Query: 601 LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           LYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA ASRNIFHLVWG GK
Sbjct: 601 LYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINAAASRNIFHLVWGSGK 650

BLAST of Lsi04G000510 vs. NCBI nr
Match: XP_011655894.1 (crossover junction endonuclease MUS81 isoform X2 [Cucumis sativus])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 592/652 (90.80%), Postives = 623/652 (95.55%), Query Frame = 0

Query: 1   MDRQRSPVV-CPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSM 60
           MDRQRSP V CPENEDLA FMWQKRLEMA TPKGISDKVDMTLYKAY N+CNSK  I SM
Sbjct: 1   MDRQRSPAVFCPENEDLAEFMWQKRLEMAATPKGISDKVDMTLYKAYLNVCNSKVSITSM 60

Query: 61  KDLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLIT 120
           KDLSQIKGVGKWILKQ+QDFFKSGS+GS PEELAGKGKKTKGT+RYVPQKNSVAYALLIT
Sbjct: 61  KDLSQIKGVGKWILKQVQDFFKSGSDGSEPEELAGKGKKTKGTRRYVPQKNSVAYALLIT 120

Query: 121 LYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTL 180
           LYRGTTNGNEYMRKQELIDAAEASGLSRVAI PEKGKGK SQFGSS RDWYSGWSCMKTL
Sbjct: 121 LYRGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKGKGKPSQFGSSSRDWYSGWSCMKTL 180

Query: 181 ISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSA 240
           ISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLSS+KNFDNTKGSSILESCDIS+HSA
Sbjct: 181 ISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLSSSKNFDNTKGSSILESCDISNHSA 240

Query: 241 NYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDIS 300
           NYLDSDVEVTSPS+S+S+++KSIDVPLESLERF+RMGYSK+QV++SFKEVS+DNPNKDIS
Sbjct: 241 NYLDSDVEVTSPSVSQSTKHKSIDVPLESLERFMRMGYSKHQVINSFKEVSKDNPNKDIS 300

Query: 301 SLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSN 360
           SLWPAVLCRLREDLVYGQVEA+GRENRSMGSS   G+ PK VVN +G+RSF+ RACSSSN
Sbjct: 301 SLWPAVLCRLREDLVYGQVEAIGRENRSMGSSLE-GSEPKNVVNVNGERSFSSRACSSSN 360

Query: 361 PSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENIC 420
           PSI ESRRD VK N NILSMPPLSFGEKFED YNVVLILDDREQFA HSSRSRRMIENIC
Sbjct: 361 PSIIESRRDSVKGNTNILSMPPLSFGEKFEDCYNVVLILDDREQFAKHSSRSRRMIENIC 420

Query: 421 SQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKL 480
           SQFKIQIEIRRLPVGDGIW+ARHKHLDNEYVLDFIVERK+++DLRCSIRDNRYRDQKLKL
Sbjct: 421 SQFKIQIEIRRLPVGDGIWVARHKHLDNEYVLDFIVERKDVEDLRCSIRDNRYRDQKLKL 480

Query: 481 LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAI 540
           LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLT AI
Sbjct: 481 LRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTHAI 540

Query: 541 TQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL 600
           TQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL
Sbjct: 541 TQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVL 600

Query: 601 DLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK 652
           DLYPTLLSL RAYSLLDGNVTEQEEMLRR+SNNLINAGASRNIFHLVWGCGK
Sbjct: 601 DLYPTLLSLVRAYSLLDGNVTEQEEMLRRRSNNLINAGASRNIFHLVWGCGK 651

BLAST of Lsi04G000510 vs. TAIR 10
Match: AT4G30870.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.9e-196
Identity = 365/668 (54.64%), Postives = 472/668 (70.66%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E AE PKG+S+ ++ T  K Y ++C++K PI ++K
Sbjct: 1   MDDERR-VLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGK--GKKTKGTKRYVPQKNSVAYALLI 120
           DLSQIKG GKW++K ++ +F +  E S  E+L     GKK  G KRY+PQ+NSV YALLI
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKT 180
           TL+R TTNG E+MRKQELIDAA+A+GLS   +GPEKGKGKA   G S R+WYSGWSCM T
Sbjct: 121 TLHRRTTNGKEFMRKQELIDAADANGLSHAPVGPEKGKGKAG-LGHSKREWYSGWSCMTT 180

Query: 181 LISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTK--NFDNTKGSSILESCDISD 240
           LI +GLVVKS+ PAKYMLT EG+ VA EC++RSGL  S    + D    +   +     +
Sbjct: 181 LIQKGLVVKSSNPAKYMLTVEGREVANECILRSGLPDSVDILSVDEMDPTPQAKKTPNQN 240

Query: 241 HSANY-LDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPN 300
            + ++ +  ++    P     S      +P + LE+F   GYSK QV+ +F+EVS+ + +
Sbjct: 241 PTCSFTMREELPYVDPRCRAQSA-----IPSDILEKFTPFGYSKEQVVAAFREVSDGSGD 300

Query: 301 KDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRAC 360
           KD S+LW +V+C LR+  VY    +      S   SS         V+ +G R+   R+C
Sbjct: 301 KDPSTLWLSVMCHLRQAEVY---NSCPDSRNSKKDSSGPFKSQIRQVDLEGSRAKKFRSC 360

Query: 361 ---SSSNPSITESRR-----------DGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDR 420
              S+ NP  + S             DG K   NI  +PPL FGE FE++Y+V+LILDDR
Sbjct: 361 NDGSTLNPCSSGSSHAVKACSSSLASDGTKGITNIPRLPPLQFGETFEEAYDVILILDDR 420

Query: 421 EQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNID 480
           E+FA   SRSR ++ENICS+F I+IE+RRLPVGD IWIARHK+L+ EYVLDFI ERKN+D
Sbjct: 421 EKFATKGSRSRNIVENICSEFNIKIEVRRLPVGDCIWIARHKYLETEYVLDFIAERKNVD 480

Query: 481 DLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQR 540
           D+R SIRDNRYRDQKL+L R G K++IY++EGDPN S+AAESIKTACFTTEILEGFDV R
Sbjct: 481 DMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDPNHSDAAESIKTACFTTEILEGFDVLR 540

Query: 541 TSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDA-CPRLNEFIKKCQELDKMTVSDVF 600
           T GL +TL+KYGYLT++I QYY L+ +D   S   A CP  + F+K+CQ+LDKMT+SDVF
Sbjct: 541 THGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKGAASCPSFDSFVKRCQDLDKMTISDVF 600

Query: 601 ALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASR 649
           A+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS L+ +V+ QEEMLR +SNN+I A AS+
Sbjct: 601 AIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSHLEADVSAQEEMLRNRSNNVICASASK 658

BLAST of Lsi04G000510 vs. TAIR 10
Match: AT5G39770.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 8.0e-123
Identity = 276/628 (43.95%), Postives = 361/628 (57.48%), Query Frame = 0

Query: 31   PKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKWILKQIQDFFKSGSEGSGPE 90
            PKG+S+  +M   KAY N+C++K  I ++KDLS+IKG GK+++K +Q +F +G+  S  E
Sbjct: 762  PKGLSEYQEMKFVKAYRNVCDAKDSIDTLKDLSKIKGFGKYMVKLMQGYFVTGAASSEQE 821

Query: 91   ELAGK--GKKTKGTKRYVPQKNSVAYALLITLYR-GTTNGNEYMRKQELIDAAEASGLSR 150
             L G   GKK KG++ Y PQ+N+ AYALL TL+R  T NG + MRKQ+L+DAA+ASGLS 
Sbjct: 822  ALPGNRAGKKAKGSEPYKPQRNTAAYALL-TLHRFKTANGKKLMRKQKLMDAADASGLSH 881

Query: 151  VAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVAREC 210
            V+I PEKGKGKA   G+S R+WYSGWSCM  LI +GLVVK + PAKYMLT EG++VA + 
Sbjct: 882  VSIAPEKGKGKAG-LGTSKREWYSGWSCMSKLIEKGLVVKFSNPAKYMLTVEGRNVADDL 941

Query: 211  LMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLE 270
              RS            +G S + +   S  +  +L +D                      
Sbjct: 942  DPRS------------RGQSSIPA-GPSSRAQEFLATDT--------------------- 1001

Query: 271  SLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRS 330
                                                            GQV+  G   + 
Sbjct: 1002 ------------------------------------------------GQVDLEGSRAKK 1061

Query: 331  MGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEK 390
              SSS   +            S  L+ACSSS  S      DG +    I  +PPL FGE 
Sbjct: 1062 FRSSSPCSS----------SISHALKACSSSLAS------DGTEGVTTIPRLPPLRFGEA 1121

Query: 391  FEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDN 450
            FE+ Y+V+LILDDREQFA                                    HK+  +
Sbjct: 1122 FEEVYDVILILDDREQFATKG---------------------------------HKYQQD 1181

Query: 451  EYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTA 510
            EYVLDFIVERKN+DD+R SI DNRY+DQKL+L R G+K++IY++EGDPN S+ AESIKTA
Sbjct: 1182 EYVLDFIVERKNVDDMRSSIMDNRYKDQKLRLQRSGIKKLIYILEGDPNQSKTAESIKTA 1241

Query: 511  CFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKK 570
            CFTTEILEGFDV RT+GL +TL+KYGYLT++I QYY    +D++   +D+CP  ++F+++
Sbjct: 1242 CFTTEILEGFDVVRTNGLGETLRKYGYLTKSIYQYYKSLVNDDQRKVADSCPSYDDFVQR 1255

Query: 571  CQELDKMTVSDVFALQLMQVPQVTEE---------VAVAVLDLYPTLLSLARAYSLLDGN 630
            C +L KMT+SDVFA+QLMQV  + +           A+AVLD Y TL SLA AYS LD N
Sbjct: 1302 CPDLGKMTISDVFAIQLMQVLSLAKPSFGFLSATFFAIAVLDKYKTLRSLASAYSKLD-N 1255

Query: 631  VTEQEEMLRRQSNNLINAGASRNIFHLV 647
            V+ QEEMLR QSNN+I A AS+NI+ LV
Sbjct: 1362 VSAQEEMLRNQSNNVICASASKNIYKLV 1255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5W9E72.6e-19554.64Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 P... [more]
Q8GT069.1e-15649.10Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6BJ484.7e-3525.39Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 3623... [more]
Q6CPQ82.6e-3323.29Crossover junction endonuclease MUS81 OS=Kluyveromyces lactis (strain ATCC 8585 ... [more]
Q7SXA92.4e-3125.97Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BFN60.0e+0092.01crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0KWV20.0e+0090.80ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 ... [more]
A0A6J1GYZ80.0e+0091.24crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A5A7SUV80.0e+0090.94Crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
A0A6J1JV850.0e+0090.78crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC11148863... [more]
Match NameE-valueIdentityDescription
XP_038892703.10.0e+0094.33crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida][more]
XP_008446914.10.0e+0092.01PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_0... [more]
XP_023514312.10.0e+0091.40crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo][more]
XP_022956424.10.0e+0091.24crossover junction endonuclease MUS81 [Cucurbita moschata][more]
XP_011655894.10.0e+0090.80crossover junction endonuclease MUS81 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G30870.11.9e-19654.64Restriction endonuclease, type II-like superfamily protein [more]
AT5G39770.18.0e-12343.95Restriction endonuclease, type II-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006166ERCC4 domainSMARTSM00891ERCC4_2coord: 395..495
e-value: 5.0E-21
score: 85.9
IPR006166ERCC4 domainPFAMPF02732ERCC4coord: 399..529
e-value: 5.7E-19
score: 68.8
NoneNo IPR availableGENE3D3.40.50.10130coord: 388..539
e-value: 5.3E-27
score: 96.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..102
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 97..219
e-value: 1.0E-30
score: 107.7
IPR042530EME1/EME2, C-terminal domainGENE3D1.10.150.670coord: 574..649
e-value: 4.9E-12
score: 48.2
IPR033309Crossover junction endonuclease Mus81PANTHERPTHR13451CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81coord: 9..648
IPR011335Restriction endonuclease type II-likeSUPERFAMILY52980Restriction endonuclease-likecoord: 392..538

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000510.1Lsi04G000510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0000737 DNA catabolic process, endonucleolytic
biological_process GO:0000727 double-strand break repair via break-induced replication
biological_process GO:0031573 mitotic intra-S DNA damage checkpoint signaling
biological_process GO:0000712 resolution of meiotic recombination intermediates
biological_process GO:0006302 double-strand break repair
cellular_component GO:0048476 Holliday junction resolvase complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0048257 3'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0004518 nuclease activity