Lsi04G000400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G000400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAnnexin
Locationchr04: 556763 .. 560390 (-)
RNA-Seq ExpressionLsi04G000400
SyntenyLsi04G000400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATTTTTAATTTTGAATCGCAGCAAAAAGAAATGCTCTATTGATTTTGGAACTAAAAAAACATTAATTTGCACTTTATAAGTACATAAAAAACAGAAGAAGAGAAAAGAGAGAGATGTAATTAAGAAAGTGGTTTTAATTGTTATAAAACGAAAATATTAAAATTGTTCGGACAAAGAAGAGCGAAATGACGAAAATGGGCATATTGAGTTTGTTTTATAACGCGGGAGGAATTAGATGAGGTGATTGTACTCTGCTTTCTTCTTCTTCTTCTTCGCTTTCGGCTTCTCTGCGATTCAATAAGCAATTCAGAAATGGCGGATTCTGCCGTAGAAGTTCTCACTCGAGCTCTCTCAGGTTAACTCTTCATTCCTTCTTCTTTCATTACTCCGCCATTCATTTTCTCTTTCTCATTGTTTTTTTTTTTCATTTTTCTCCTCTTGATTGCAAAATTCATCGAAATCTGTTTATCGATCTTCATTTTTCTTCATTCGGAGTATGTTTTTTTAGTTTTCGATCGAAAATTCCACTCTTCGCCGGAGAAATAAGATCGCTGGCCTCGACGTCGGTGTCTCTATGTTTTTCTTACTCCGTCGCAAAACCTTCATAAACTCTTTGGTCTTCGCCGGAGAAATACAACTTCTGGCCACGATCACAGTTTCTCTCTGTTTTCTTACAGATCGTTTTCGTTGAAATTTACTTAAAAAATCGTTATCACTTATATGCAATTTTGTGCTAAGATTTTGTATTTCTATTTATTGATTTTTTGTTAAACTCTGTGCAATTTACAGATAATGCAATCTGGATTCTGGAATCCCTTTTCAGTATTAATTTTTAATCGAATTACCTTTATGCCCCTGCAGTTTTTTTTTTTTGAGAAATTTTTAAAAAAATAAAAATAAAGAAAACTATTTACACAAAATAGCAAAGATAGTTGTAATAGACTTATTAGAGTCTATGAATGATAGAAATGATAGAAGTATATCACTAATAGATGTTGATAGAAGTTTATCAATATCTATCAGTATTTTTTTTTTTTTTTTTTTGCTATTTTATGTAAATAGACATTTTTTTAGGAAAATTTTCATAAATAGAAAAAATGTCAAACTATTTACAAAAAATAGCAAAAAAAAATATTGATAGACATTGATAGACTTCTATCATTTATATCACTGATAGACTCTGATAGACTTCTATCAGCGTCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTCCTCATTTTTCTATTTTTAAAAACTTCCCTTTTTTATCGGTAAAAATTTTCTCTTTTTTTCCCTTTTAGTTTCTTAATCTTAAAAAATTAGTTTAAAATGGTTGTGTTGTAGTAACGTTAGCCAAGTGTGGTCCATTGATATTATTTGATTGACCAAGAGTAATTTTTTTCCTCTTTTTTTAAAGAAAACTAATTGTCAAATTTTCTATTTAAATTTAATTTAAGTTAATAATTTTATCTTGGAGGTTGAGAAAATTCCACTACTTTAATATTTTTTTGGGCAATGAAAAAATTGTAATGGTTACCCAAATATGACATGTCTACAAGTTAATCGATCTTTTTTATTTACACGATACCTATTGAATATAAGAGGTGCTATTTATTGACAATTTTATTTATAGAAATCTCAAATCAATTCACCAACGTTTTTTTTTTTTAATTTGTAAAGTTGTTTTGTGTTGTAGCTCAATTTCTAAAAGAAAAAAAAAAATTTAACTGTATAGTTTTACTTTTAATTTCATCTAAAAACTTCATACATTTAAGAAAAGTTTTCACGATTTATGTACCATGGATCTTCCCCTTATCTAGTAGGATCAGACTAATTCATTCTTAAAACCGAATGAATATGATTCTTAGAAAGATAAATTATTTTGGGAAGTGAGAATATATCACATATTTAATAATGTTGAGAAACGTGAAATGAAAAACAGGGCATGGAATAAATGAGAAGGCAATGATAGACACATTAGGAAAATGGGATCATGAGGAGAAGAAATTGTTCAGAAAGAGAAGTAGCCATTTTTTCAGTGAAGATGAACGCTCCTTTGAGCGATGGCAAGAACATGGCATGAGACTTCTCAAGCATGAATTCATGCGCTTTAAGGTATCCTTTTTTCTTTTTTTTTTAAAAAAATGTTAGAATTTTGAAACCAAATTAAAGCTAAAGGTTTATATAAATATACATGTATGTATGATTTAGAATGCTGTGGTGCTATGGACAACACATCCATGGGAAAGAGATGCTCGTTTGGTAAAGGAAGCATTGAGCAAAGGGAATCATGGCCAAAACATAAACATTCTAATAGAAGTGGCTTGTACTAGAACTTCTGATGAGCTTTTAGGAGCTAGGAAAGCTTACCATTCCCTCTTTGACCATTCCATTGAAGAAGATGTTGCCAGCCACATTAATGGCCCTGAGCGCAAGGTTTAACACTAATTCCTATTCACTTTTTCACTTACTCCTTTCTTTATTACAAAATTGTGTAAACAAACCGTAGAATATATTGAATGCTTACCAAAGACTAAATAGTTAACAAATGGGATGTATCGTGTTACTTTGGAATGCAGCTTTTGGTTGCATTAATGAGTGCATATAGATACGAAGGACCAAAGTATAAGGAGGAGACAGCAAAATCTGAAGCAAAAAAGCTTGCTCATTTAATTAAGGAAGCAGCCAGCAAAAAGAGCAGCCTCATTGAAGATGAAGAGGTTGTGAGAATACTCTCCACAAGAAGCAAACACCATCTTCATGCCCTTTACAAACACTACAAGGAAATTTCAGCAGGTCGCTCCATTGATGAGGTACCTAATATAATCTCTAATGATAAACTGATGTATGAATGAGAAATATTTGCTAAGGTTTGGGATTTTCTTTTTCTTTCTTTTGGCAGGATCTTCATGGGGACTTGAGGCTTCAAGAGGCAGTGTTGTGCTTAGCCAATCCTGTTAAGTATTTCACTCAGGTATGTAAATTTCAAACTCACTTGCTTTTTGCAATAAAATCGAAATTTTCATATTTTTCTTTGATTGTTACTAAATAATGTCATTTCTTATTTTGTGTTTAGGTTCTGAACGTGTCATTAAAAGTCGACGCGGACAAGAAGATAAAAAAGGTATTGACTCGAATCGTCGTTACGAGAGCCGATAAAGACATGAAGGAGATCAAAGTTGAGTTCAAAAATCAGTTTGGAGTTTCATTGGCTGAGAAAATTGAGTCTGTGTGCAATGGTAGCTACAAAGATTTCTTGCTCACTTTGCTGGCAAGATCAGACTAATTATTATGTGAAGATGAATAAAAAGATTTGAAAATTGCAGTTTTTTTTTTCACTTAGAAGATCGAAATTTGCTGTGTTTCATTTACATGTATGAGTTGGTCGTTTTTCATGATTCCCTATTGCTTATGAGGCTGTGTTATTCTCTTTTGCCATAATAAATTTGAACATTGAAAAGTGTTCACATATTTGTTTGTTCAATTATTATATATACCCTTTGTATTGTGCATTCTATTTCATGAACACAATATCATTTTTAAACTTTCTTCTAAATCAAAATATGCAACTATATAATATATATCAAAGTGTCATGTTAACTTTCATTAGC

mRNA sequence

TTTATTTTTAATTTTGAATCGCAGCAAAAAGAAATGCTCTATTGATTTTGGAACTAAAAAAACATTAATTTGCACTTTATAAGTACATAAAAAACAGAAGAAGAGAAAAGAGAGAGATGTAATTAAGAAAGTGGTTTTAATTGTTATAAAACGAAAATATTAAAATTGTTCGGACAAAGAAGAGCGAAATGACGAAAATGGGCATATTGAGTTTGTTTTATAACGCGGGAGGAATTAGATGAGGTGATTGTACTCTGCTTTCTTCTTCTTCTTCTTCGCTTTCGGCTTCTCTGCGATTCAATAAGCAATTCAGAAATGGCGGATTCTGCCGTAGAAGTTCTCACTCGAGCTCTCTCAGGGCATGGAATAAATGAGAAGGCAATGATAGACACATTAGGAAAATGGGATCATGAGGAGAAGAAATTGTTCAGAAAGAGAAGTAGCCATTTTTTCAGTGAAGATGAACGCTCCTTTGAGCGATGGCAAGAACATGGCATGAGACTTCTCAAGCATGAATTCATGCGCTTTAAGAATGCTGTGGTGCTATGGACAACACATCCATGGGAAAGAGATGCTCGTTTGGTAAAGGAAGCATTGAGCAAAGGGAATCATGGCCAAAACATAAACATTCTAATAGAAGTGGCTTGTACTAGAACTTCTGATGAGCTTTTAGGAGCTAGGAAAGCTTACCATTCCCTCTTTGACCATTCCATTGAAGAAGATGTTGCCAGCCACATTAATGGCCCTGAGCGCAAGCTTTTGGTTGCATTAATGAGTGCATATAGATACGAAGGACCAAAGTATAAGGAGGAGACAGCAAAATCTGAAGCAAAAAAGCTTGCTCATTTAATTAAGGAAGCAGCCAGCAAAAAGAGCAGCCTCATTGAAGATGAAGAGGTTGTGAGAATACTCTCCACAAGAAGCAAACACCATCTTCATGCCCTTTACAAACACTACAAGGAAATTTCAGCAGGTCGCTCCATTGATGAGGATCTTCATGGGGACTTGAGGCTTCAAGAGGCAGTGTTGTGCTTAGCCAATCCTGTTAAGTATTTCACTCAGGTTCTGAACGTGTCATTAAAAGTCGACGCGGACAAGAAGATAAAAAAGGTATTGACTCGAATCGTCGTTACGAGAGCCGATAAAGACATGAAGGAGATCAAAGTTGAGTTCAAAAATCAGTTTGGAGTTTCATTGGCTGAGAAAATTGAGTCTGTGTGCAATGGTAGCTACAAAGATTTCTTGCTCACTTTGCTGGCAAGATCAGACTAATTATTATGTGAAGATGAATAAAAAGATTTGAAAATTGCAGTTTTTTTTTTCACTTAGAAGATCGAAATTTGCTGTGTTTCATTTACATGTATGAGTTGGTCGTTTTTCATGATTCCCTATTGCTTATGAGGCTGTGTTATTCTCTTTTGCCATAATAAATTTGAACATTGAAAAGTGTTCACATATTTGTTTGTTCAATTATTATATATACCCTTTGTATTGTGCATTCTATTTCATGAACACAATATCATTTTTAAACTTTCTTCTAAATCAAAATATGCAACTATATAATATATATCAAAGTGTCATGTTAACTTTCATTAGC

Coding sequence (CDS)

ATGGCGGATTCTGCCGTAGAAGTTCTCACTCGAGCTCTCTCAGGGCATGGAATAAATGAGAAGGCAATGATAGACACATTAGGAAAATGGGATCATGAGGAGAAGAAATTGTTCAGAAAGAGAAGTAGCCATTTTTTCAGTGAAGATGAACGCTCCTTTGAGCGATGGCAAGAACATGGCATGAGACTTCTCAAGCATGAATTCATGCGCTTTAAGAATGCTGTGGTGCTATGGACAACACATCCATGGGAAAGAGATGCTCGTTTGGTAAAGGAAGCATTGAGCAAAGGGAATCATGGCCAAAACATAAACATTCTAATAGAAGTGGCTTGTACTAGAACTTCTGATGAGCTTTTAGGAGCTAGGAAAGCTTACCATTCCCTCTTTGACCATTCCATTGAAGAAGATGTTGCCAGCCACATTAATGGCCCTGAGCGCAAGCTTTTGGTTGCATTAATGAGTGCATATAGATACGAAGGACCAAAGTATAAGGAGGAGACAGCAAAATCTGAAGCAAAAAAGCTTGCTCATTTAATTAAGGAAGCAGCCAGCAAAAAGAGCAGCCTCATTGAAGATGAAGAGGTTGTGAGAATACTCTCCACAAGAAGCAAACACCATCTTCATGCCCTTTACAAACACTACAAGGAAATTTCAGCAGGTCGCTCCATTGATGAGGATCTTCATGGGGACTTGAGGCTTCAAGAGGCAGTGTTGTGCTTAGCCAATCCTGTTAAGTATTTCACTCAGGTTCTGAACGTGTCATTAAAAGTCGACGCGGACAAGAAGATAAAAAAGGTATTGACTCGAATCGTCGTTACGAGAGCCGATAAAGACATGAAGGAGATCAAAGTTGAGTTCAAAAATCAGTTTGGAGTTTCATTGGCTGAGAAAATTGAGTCTGTGTGCAATGGTAGCTACAAAGATTTCTTGCTCACTTTGCTGGCAAGATCAGACTAA

Protein sequence

MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCLANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIESVCNGSYKDFLLTLLARSD
Homology
BLAST of Lsi04G000400 vs. ExPASy Swiss-Prot
Match: Q9ZVJ6 (Annexin D4 OS=Arabidopsis thaliana OX=3702 GN=ANN4 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-85
Identity = 174/319 (54.55%), Postives = 230/319 (72.10%), Query Frame = 0

Query: 6   VEVLTRALS---GHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSED-ERSFERWQEHGM 65
           +E LT A+S   G G++E A+I TLGK   E +KLFRK S  FF ED ER+FE+  +H +
Sbjct: 7   LESLTEAISAGMGMGVDENALISTLGKSQKEHRKLFRKASKSFFVEDEERAFEKCHDHFV 66

Query: 66  RLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGA 125
           R LK EF RF  AVV+W  HPWERDARLVK+AL KG   +  N+++EV+CTR++++LLGA
Sbjct: 67  RHLKLEFSRFNTAVVMWAMHPWERDARLVKKALKKGE--EAYNLIVEVSCTRSAEDLLGA 126

Query: 126 RKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKE 185
           RKAYHSLFD S+EED+ASH++GP+RKLLV L+SAYRYEG K K+++AKS+AK LA  +  
Sbjct: 127 RKAYHSLFDQSMEEDIASHVHGPQRKLLVGLVSAYRYEGNKVKDDSAKSDAKILAEAV-- 186

Query: 186 AASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCLA 245
            AS     +E +EVVRIL+TRSK HL  LYKH+ EI  G  +   +     L EA++CL 
Sbjct: 187 -ASSGEEAVEKDEVVRILTTRSKLHLQHLYKHFNEIK-GSDLLGGVSKSSLLNEALICLL 246

Query: 246 NPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRAD--KDMKEIKVEFKNQFGVSLAEKIE 305
            P  YF+++L+ SL  DADK  KK LTR+ VTRAD   +M EIK E+ N +G +LA++I+
Sbjct: 247 KPALYFSKILDASLNKDADKTTKKWLTRVFVTRADHSDEMNEIKEEYNNLYGETLAQRIQ 306

Query: 306 SVCNGSYKDFLLTLLARSD 319
               G+Y+DFLLTLL++SD
Sbjct: 307 EKIKGNYRDFLLTLLSKSD 319

BLAST of Lsi04G000400 vs. ExPASy Swiss-Prot
Match: P51074 (Annexin-like protein RJ4 OS=Fragaria ananassa OX=3747 PE=2 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 6.0e-44
Identity = 120/324 (37.04%), Postives = 180/324 (55.56%), Query Frame = 0

Query: 2   ADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGM 61
           A    E L +++ G G NEKA+I  LG  +  ++K  R      + ED           +
Sbjct: 12  AKEDAEALRKSVKGWGTNEKAIISILGHRNAGQRKEIRAAYEQLYQEDL----------L 71

Query: 62  RLLKHEFM-RFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 121
           + L+ E    F+ AV  WT  P +RDA L   A+ K       N++IE++C  + +ELL 
Sbjct: 72  KPLESELSGDFEKAVYRWTLDPADRDAVLANVAIKKST--DVYNVIIEISCIHSPEELLA 131

Query: 122 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 181
            R+AY   + HS+EED+A+H  G  RKLLVAL++AYRY+G +   + A SEA  L   IK
Sbjct: 132 VRRAYQLRYKHSVEEDLAAHTTGDIRKLLVALVTAYRYDGHEINAKLANSEADILHDAIK 191

Query: 182 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKE---ISAGRSIDEDLHGDLR--LQE 241
           + A         EE++RILSTRSK  L A +  Y++   IS  +++ E+   D +  L  
Sbjct: 192 DKA------FNHEEIIRILSTRSKTQLMATFNKYRDDQGISISKNLLEEGANDFQKALHT 251

Query: 242 AVLCLANPVKYFTQVLNVSLK-VDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSL 301
           A+ CL +P KYF +VL  ++K V  D   +  LTR++VTRA++D+++IK  +  +  V L
Sbjct: 252 AIRCLNDPKKYFEKVLRNAIKRVGTD---EDALTRVIVTRAERDLRDIKEVYYKKNSVPL 311

Query: 302 AEKIESVCNGSYKDFLLTLLARSD 319
            + +    +G YK FLLTLL + D
Sbjct: 312 EQAVAKDTSGDYKAFLLTLLGKED 314

BLAST of Lsi04G000400 vs. ExPASy Swiss-Prot
Match: P93157 (Annexin Gh1 (Fragment) OS=Gossypium hirsutum OX=3635 GN=AnnGh1 PE=1 SV=2)

HSP 1 Score: 173.7 bits (439), Expect = 3.3e-42
Identity = 119/314 (37.90%), Postives = 172/314 (54.78%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E L +A SG G NE  +ID LG  + E++ L RK  +  + ED           ++ L  
Sbjct: 17  EQLRKAFSGWGTNEGLIIDILGHRNAEQRNLIRKTYAETYGEDL----------LKALDK 76

Query: 67  EFMR-FKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAY 126
           E    F+  V+LW   P ERDA L  EA  +        +L+E+ACTR++++LL AR+AY
Sbjct: 77  ELSNDFERLVLLWALDPAERDALLANEATKRWTSSN--QVLMEIACTRSANQLLHARQAY 136

Query: 127 HSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASK 186
           H+ +  S+EEDVA H  G   KLL+ L+S+YRYEG +     AK+EAK    L+ E  S 
Sbjct: 137 HARYKKSLEEDVAHHTTGDFHKLLLPLVSSYRYEGEEVNMTLAKTEAK----LLHEKISN 196

Query: 187 KSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLR------LQEAVLC 246
           K+    D++V+R+L+TRSK  ++A   HYK    G  I++DL  D +      L+  V C
Sbjct: 197 KA--YSDDDVIRVLATRSKAQINATLNHYKN-EYGNDINKDLKADPKDEFLALLRSTVKC 256

Query: 247 LANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIE 306
           L  P KYF +VL   L ++     +  LTR+V TRA+ D+K I  E++ +  V L   I 
Sbjct: 257 LVYPEKYFEKVLR--LAINRRGTDEGALTRVVCTRAEVDLKVIADEYQRRNSVPLTRAIV 309

Query: 307 SVCNGSYKDFLLTL 314
              +G Y+  LL L
Sbjct: 317 KDTHGDYEKLLLVL 309

BLAST of Lsi04G000400 vs. ExPASy Swiss-Prot
Match: Q9SYT0 (Annexin D1 OS=Arabidopsis thaliana OX=3702 GN=ANN1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-40
Identity = 116/320 (36.25%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E L  A  G G NE  +I  L     E++K+ R+     + ED           ++ L  
Sbjct: 18  EQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGEDL----------LKTLDK 77

Query: 67  EFMR-FKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAY 126
           E    F+ A++LWT  P ERDA L  EA  +        +L+EVACTRTS +LL AR+AY
Sbjct: 78  ELSNDFERAILLWTLEPGERDALLANEATKRWTSSN--QVLMEVACTRTSTQLLHARQAY 137

Query: 127 HSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASK 186
           H+ +  S+EEDVA H  G  RKLLV+L+++YRYEG +     AK EAK +   IK+    
Sbjct: 138 HARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKLVHEKIKD---- 197

Query: 187 KSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGD------LRLQEAVLC 246
                 DE+V+RILSTRSK  ++A +  Y++      +     GD        L+  + C
Sbjct: 198 --KHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLRSTIQC 257

Query: 247 LANPVKYFTQVLNVSL-KVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKI 306
           L  P  YF  VL  ++ K   D   +  LTRIV TRA+ D+K I  E++ +  + L + I
Sbjct: 258 LTRPELYFVDVLRSAINKTGTD---EGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEKAI 316

Query: 307 ESVCNGSYKDFLLTLLARSD 319
                G Y+  L+ LL   D
Sbjct: 318 TKDTRGDYEKMLVALLGEDD 316

BLAST of Lsi04G000400 vs. ExPASy Swiss-Prot
Match: Q94CK4 (Annexin D8 OS=Arabidopsis thaliana OX=3702 GN=ANNAT8 PE=2 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 5.3e-40
Identity = 119/316 (37.66%), Postives = 172/316 (54.43%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E +  A  G G NE A+I  LG  +  ++KL R+     + ED           +  LK 
Sbjct: 17  ENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHEDL----------IHQLKS 76

Query: 67  EFM-RFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAY 126
           E    F+ A+ LW   P ERDA L   AL K     +  +L+E+AC R+ +++L AR+AY
Sbjct: 77  ELSGNFERAICLWVLDPPERDALLANLALQKPI--PDYKVLVEIACMRSPEDMLAARRAY 136

Query: 127 HSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASK 186
             L+ HS+EED+AS   G  R+LLVA++SAY+Y+G +  E  A+SEA   A L  E   K
Sbjct: 137 RCLYKHSLEEDLASRTIGDIRRLLVAMVSAYKYDGEEIDEMLAQSEA---AILHDEILGK 196

Query: 187 KSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGD------LRLQEAVLC 246
               ++ EE +R+LSTRS   L A++  YK+I  G SI +DL           L+ A+ C
Sbjct: 197 ---AVDHEETIRVLSTRSSMQLSAIFNRYKDI-YGTSITKDLLNHPTNEYLSALRAAIRC 256

Query: 247 LANPVKYFTQVLNVSLK-VDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKI 306
           + NP +Y+ +VL  S+  V  D   +  L R++VTRA+KD+  I   +  +  VSL + I
Sbjct: 257 IKNPTRYYAKVLRNSINTVGTD---EDALNRVIVTRAEKDLTNITGLYFKRNNVSLDQAI 310

Query: 307 ESVCNGSYKDFLLTLL 315
               +G YK FLL LL
Sbjct: 317 AKETSGDYKAFLLALL 310

BLAST of Lsi04G000400 vs. ExPASy TrEMBL
Match: A0A0A0KRR1 (Annexin 11 OS=Cucumis sativus OX=3659 GN=Csa_5G623670 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 296/318 (93.08%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSAVEVLTRALSGHGINE AMI+TLGKWDHEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAVEVLTRALSGHGINENAMIETLGKWDHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHAL+KHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALHKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+LNVSLK DADKKIKKVLTRIVVTRAD DMKEIKVEFK QFG+SLAEKI S
Sbjct: 241 TNPVKYFTQLLNVSLKADADKKIKKVLTRIVVTRADNDMKEIKVEFKKQFGISLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. ExPASy TrEMBL
Match: A0A5A7SZK1 (Annexin D4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002980 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.7e-163
Identity = 294/318 (92.45%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINE AMI+TLGKW+HEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINENAMIETLGKWNHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHALYKHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALYKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+L+VSLK DADKKIKKVLTR+VVTRAD DMKEIKVEFK QFGVSLAEKI S
Sbjct: 241 TNPVKYFTQLLDVSLKADADKKIKKVLTRVVVTRADNDMKEIKVEFKKQFGVSLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. ExPASy TrEMBL
Match: E5GCK4 (Annexin OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.7e-163
Identity = 294/318 (92.45%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINE AMI+TLGKW+HEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINENAMIETLGKWNHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHALYKHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALYKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+L+VSLK DADKKIKKVLTR+VVTRAD DMKEIKVEFK QFGVSLAEKI S
Sbjct: 241 TNPVKYFTQLLDVSLKADADKKIKKVLTRVVVTRADNDMKEIKVEFKKQFGVSLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. ExPASy TrEMBL
Match: A0A1S3BH44 (annexin D4 OS=Cucumis melo OX=3656 GN=LOC103489495 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.7e-163
Identity = 294/318 (92.45%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINE AMI+TLGKW+HEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINENAMIETLGKWNHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHALYKHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALYKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+L+VSLK DADKKIKKVLTR+VVTRAD DMKEIKVEFK QFGVSLAEKI S
Sbjct: 241 TNPVKYFTQLLDVSLKADADKKIKKVLTRVVVTRADNDMKEIKVEFKKQFGVSLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. ExPASy TrEMBL
Match: A0A6J1CB97 (annexin D4 OS=Momordica charantia OX=3673 GN=LOC111010078 PE=4 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.7e-158
Identity = 284/318 (89.31%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINEK MI+TLGKWDHEEKKLFRKRSSH+FSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINEKVMIETLGKWDHEEKKLFRKRSSHYFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+H QNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHHQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASHI+GPERKLLVALMSAYRYEGPK+K+ETAKSEAKK+A  IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHIHGPERKLLVALMSAYRYEGPKFKDETAKSEAKKVAQAIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EAAS+KSSLIE++++VRILSTRSKHH HALYKHYKEI+AG+ IDEDL  DLRLQE VLCL
Sbjct: 181 EAASRKSSLIEEDDIVRILSTRSKHHFHALYKHYKEITAGKLIDEDLREDLRLQETVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
           A+PVKYFTQ+L+VSLKVDADKKIKKVLTRIVVTRADKDMKEIKVE+KN+FG+SLAEKI +
Sbjct: 241 ADPVKYFTQILDVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEYKNRFGISLAEKIGA 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VC+GSYKDFLLTLLARSD
Sbjct: 301 VCHGSYKDFLLTLLARSD 318

BLAST of Lsi04G000400 vs. NCBI nr
Match: XP_038891533.1 (annexin D4 [Benincasa hispida])

HSP 1 Score: 594.7 bits (1532), Expect = 4.6e-166
Identity = 304/318 (95.60%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSAVEVLTRALSGHGINEKAMI+TLGKWDHEEKK+FRKRSSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAVEVLTRALSGHGINEKAMIETLGKWDHEEKKMFRKRSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEG KYKEE AKSEAKKLAH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGSKYKEEIAKSEAKKLAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA+SKKSSLIEDEEVVRILSTRSKH LHALYKHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EASSKKSSLIEDEEVVRILSTRSKHFLHALYKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
           ANPVKYFTQ+LNVSLKVDADKKIKKVLTRIVVTRAD DMKEIKVEFKNQFGVSLAEKI S
Sbjct: 241 ANPVKYFTQLLNVSLKVDADKKIKKVLTRIVVTRADTDMKEIKVEFKNQFGVSLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFLLTLLARS+
Sbjct: 301 VCNGSYKDFLLTLLARSN 318

BLAST of Lsi04G000400 vs. NCBI nr
Match: XP_004142407.1 (annexin D4 [Cucumis sativus] >KGN52303.1 hypothetical protein Csa_009205 [Cucumis sativus])

HSP 1 Score: 585.5 bits (1508), Expect = 2.8e-163
Identity = 296/318 (93.08%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSAVEVLTRALSGHGINE AMI+TLGKWDHEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAVEVLTRALSGHGINENAMIETLGKWDHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHAL+KHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALHKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+LNVSLK DADKKIKKVLTRIVVTRAD DMKEIKVEFK QFG+SLAEKI S
Sbjct: 241 TNPVKYFTQLLNVSLKADADKKIKKVLTRIVVTRADNDMKEIKVEFKKQFGISLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. NCBI nr
Match: XP_008446930.1 (PREDICTED: annexin D4 [Cucumis melo] >ADN34201.1 annexin [Cucumis melo subsp. melo] >KAA0034747.1 annexin D4 [Cucumis melo var. makuwa] >TYK09301.1 annexin D4 [Cucumis melo var. makuwa])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 294/318 (92.45%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINE AMI+TLGKW+HEEKKLFRK+SSHFFSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINENAMIETLGKWNHEEKKLFRKKSSHFFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+HGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASH+NGPERKLLVALMSAYRYEGPKYKEE AKSEAKK AH IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHLNGPERKLLVALMSAYRYEGPKYKEEIAKSEAKKFAHSIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIEDEE+VRILSTRSKH LHALYKHY EISAGRSIDEDLHGDLRLQEAVLCL
Sbjct: 181 EANSKKSSLIEDEEIVRILSTRSKHFLHALYKHYNEISAGRSIDEDLHGDLRLQEAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
            NPVKYFTQ+L+VSLK DADKKIKKVLTR+VVTRAD DMKEIKVEFK QFGVSLAEKI S
Sbjct: 241 TNPVKYFTQLLDVSLKADADKKIKKVLTRVVVTRADNDMKEIKVEFKKQFGVSLAEKIGS 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFL+TLLARSD
Sbjct: 301 VCNGSYKDFLITLLARSD 318

BLAST of Lsi04G000400 vs. NCBI nr
Match: XP_022139070.1 (annexin D4 [Momordica charantia])

HSP 1 Score: 568.5 bits (1464), Expect = 3.5e-158
Identity = 284/318 (89.31%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINEK MI+TLGKWDHEEKKLFRKRSSH+FSEDERSFERW+EHG
Sbjct: 1   MADSAIEVLTRALSGHGINEKVMIETLGKWDHEEKKLFRKRSSHYFSEDERSFERWEEHG 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKG+H QNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGHHHQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASHI+GPERKLLVALMSAYRYEGPK+K+ETAKSEAKK+A  IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHIHGPERKLLVALMSAYRYEGPKFKDETAKSEAKKVAQAIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EAAS+KSSLIE++++VRILSTRSKHH HALYKHYKEI+AG+ IDEDL  DLRLQE VLCL
Sbjct: 181 EAASRKSSLIEEDDIVRILSTRSKHHFHALYKHYKEITAGKLIDEDLREDLRLQETVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
           A+PVKYFTQ+L+VSLKVDADKKIKKVLTRIVVTRADKDMKEIKVE+KN+FG+SLAEKI +
Sbjct: 241 ADPVKYFTQILDVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEYKNRFGISLAEKIGA 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VC+GSYKDFLLTLLARSD
Sbjct: 301 VCHGSYKDFLLTLLARSD 318

BLAST of Lsi04G000400 vs. NCBI nr
Match: XP_022945018.1 (annexin D4-like [Cucurbita moschata] >KAG6573813.1 Annexin D4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.8 bits (1462), Expect = 6.0e-158
Identity = 287/318 (90.25%), Postives = 300/318 (94.34%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           MADSA+EVLTRALSGHGINEKAMIDTLG WDHE KKLFR +SSH FSEDERSFERWQEH 
Sbjct: 1   MADSAIEVLTRALSGHGINEKAMIDTLGNWDHEHKKLFRIKSSHIFSEDERSFERWQEHA 60

Query: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLG 120
           MRLLKHEFMRFKNAVVLWTTHPWERDARLV+EALSKGNHGQNINILIEVACTRTSDELLG
Sbjct: 61  MRLLKHEFMRFKNAVVLWTTHPWERDARLVREALSKGNHGQNINILIEVACTRTSDELLG 120

Query: 121 ARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIK 180
           ARKAYHSLFDHSIEEDVASHIN PERKLLVALMSAYRYEGPKYKEE AKSEA+KLA  IK
Sbjct: 121 ARKAYHSLFDHSIEEDVASHINAPERKLLVALMSAYRYEGPKYKEELAKSEAEKLARAIK 180

Query: 181 EAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCL 240
           EA SKKSSLIED+EVVRILSTRSKH LH LY HYK+ISAGRSIDEDL  DL+LQ+AVLCL
Sbjct: 181 EAGSKKSSLIEDDEVVRILSTRSKHFLHVLYNHYKKISAGRSIDEDLDWDLKLQDAVLCL 240

Query: 241 ANPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKIES 300
           ANPVKYFT +L VSLKVDADKK+KKVLTRI+VTRADKDMKEIK+EFKN+FGVSL EKIES
Sbjct: 241 ANPVKYFTHILEVSLKVDADKKVKKVLTRIIVTRADKDMKEIKIEFKNKFGVSLEEKIES 300

Query: 301 VCNGSYKDFLLTLLARSD 319
           VCNGSYKDFLLTLLARSD
Sbjct: 301 VCNGSYKDFLLTLLARSD 318

BLAST of Lsi04G000400 vs. TAIR 10
Match: AT2G38750.1 (annexin 4 )

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-86
Identity = 174/319 (54.55%), Postives = 230/319 (72.10%), Query Frame = 0

Query: 6   VEVLTRALS---GHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSED-ERSFERWQEHGM 65
           +E LT A+S   G G++E A+I TLGK   E +KLFRK S  FF ED ER+FE+  +H +
Sbjct: 7   LESLTEAISAGMGMGVDENALISTLGKSQKEHRKLFRKASKSFFVEDEERAFEKCHDHFV 66

Query: 66  RLLKHEFMRFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGA 125
           R LK EF RF  AVV+W  HPWERDARLVK+AL KG   +  N+++EV+CTR++++LLGA
Sbjct: 67  RHLKLEFSRFNTAVVMWAMHPWERDARLVKKALKKGE--EAYNLIVEVSCTRSAEDLLGA 126

Query: 126 RKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKE 185
           RKAYHSLFD S+EED+ASH++GP+RKLLV L+SAYRYEG K K+++AKS+AK LA  +  
Sbjct: 127 RKAYHSLFDQSMEEDIASHVHGPQRKLLVGLVSAYRYEGNKVKDDSAKSDAKILAEAV-- 186

Query: 186 AASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGDLRLQEAVLCLA 245
            AS     +E +EVVRIL+TRSK HL  LYKH+ EI  G  +   +     L EA++CL 
Sbjct: 187 -ASSGEEAVEKDEVVRILTTRSKLHLQHLYKHFNEIK-GSDLLGGVSKSSLLNEALICLL 246

Query: 246 NPVKYFTQVLNVSLKVDADKKIKKVLTRIVVTRAD--KDMKEIKVEFKNQFGVSLAEKIE 305
            P  YF+++L+ SL  DADK  KK LTR+ VTRAD   +M EIK E+ N +G +LA++I+
Sbjct: 247 KPALYFSKILDASLNKDADKTTKKWLTRVFVTRADHSDEMNEIKEEYNNLYGETLAQRIQ 306

Query: 306 SVCNGSYKDFLLTLLARSD 319
               G+Y+DFLLTLL++SD
Sbjct: 307 EKIKGNYRDFLLTLLSKSD 319

BLAST of Lsi04G000400 vs. TAIR 10
Match: AT1G35720.1 (annexin 1 )

HSP 1 Score: 167.9 bits (424), Expect = 1.3e-41
Identity = 116/320 (36.25%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E L  A  G G NE  +I  L     E++K+ R+     + ED           ++ L  
Sbjct: 18  EQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGEDL----------LKTLDK 77

Query: 67  EFMR-FKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAY 126
           E    F+ A++LWT  P ERDA L  EA  +        +L+EVACTRTS +LL AR+AY
Sbjct: 78  ELSNDFERAILLWTLEPGERDALLANEATKRWTSSN--QVLMEVACTRTSTQLLHARQAY 137

Query: 127 HSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASK 186
           H+ +  S+EEDVA H  G  RKLLV+L+++YRYEG +     AK EAK +   IK+    
Sbjct: 138 HARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKLVHEKIKD---- 197

Query: 187 KSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGD------LRLQEAVLC 246
                 DE+V+RILSTRSK  ++A +  Y++      +     GD        L+  + C
Sbjct: 198 --KHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLRSTIQC 257

Query: 247 LANPVKYFTQVLNVSL-KVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKI 306
           L  P  YF  VL  ++ K   D   +  LTRIV TRA+ D+K I  E++ +  + L + I
Sbjct: 258 LTRPELYFVDVLRSAINKTGTD---EGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEKAI 316

Query: 307 ESVCNGSYKDFLLTLLARSD 319
                G Y+  L+ LL   D
Sbjct: 318 TKDTRGDYEKMLVALLGEDD 316

BLAST of Lsi04G000400 vs. TAIR 10
Match: AT5G12380.1 (annexin 8 )

HSP 1 Score: 166.4 bits (420), Expect = 3.8e-41
Identity = 119/316 (37.66%), Postives = 172/316 (54.43%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E +  A  G G NE A+I  LG  +  ++KL R+     + ED           +  LK 
Sbjct: 17  ENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHEDL----------IHQLKS 76

Query: 67  EFM-RFKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELLGARKAY 126
           E    F+ A+ LW   P ERDA L   AL K     +  +L+E+AC R+ +++L AR+AY
Sbjct: 77  ELSGNFERAICLWVLDPPERDALLANLALQKPI--PDYKVLVEIACMRSPEDMLAARRAY 136

Query: 127 HSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEAASK 186
             L+ HS+EED+AS   G  R+LLVA++SAY+Y+G +  E  A+SEA   A L  E   K
Sbjct: 137 RCLYKHSLEEDLASRTIGDIRRLLVAMVSAYKYDGEEIDEMLAQSEA---AILHDEILGK 196

Query: 187 KSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGD------LRLQEAVLC 246
               ++ EE +R+LSTRS   L A++  YK+I  G SI +DL           L+ A+ C
Sbjct: 197 ---AVDHEETIRVLSTRSSMQLSAIFNRYKDI-YGTSITKDLLNHPTNEYLSALRAAIRC 256

Query: 247 LANPVKYFTQVLNVSLK-VDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGVSLAEKI 306
           + NP +Y+ +VL  S+  V  D   +  L R++VTRA+KD+  I   +  +  VSL + I
Sbjct: 257 IKNPTRYYAKVLRNSINTVGTD---EDALNRVIVTRAEKDLTNITGLYFKRNNVSLDQAI 310

Query: 307 ESVCNGSYKDFLLTLL 315
               +G YK FLL LL
Sbjct: 317 AKETSGDYKAFLLALL 310

BLAST of Lsi04G000400 vs. TAIR 10
Match: AT2G38760.1 (annexin 3 )

HSP 1 Score: 163.7 bits (413), Expect = 2.4e-40
Identity = 114/322 (35.40%), Postives = 179/322 (55.59%), Query Frame = 0

Query: 7   EVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHGMRLLKH 66
           E L +A+ G G +EKA+I  LG+ D  +++  R+     + +D           + +L  
Sbjct: 18  ETLKQAIRGWGTDEKAIIRVLGQRDQSQRRKIRESFREIYGKDL----------IDVLSS 77

Query: 67  EFM-RFKNAVVLWTTHPWERDARLVKEALSKGNHG---QNINILIEVACTRTSDELLGAR 126
           E    F  AVV WT  P ERDARLV + L+K       +N+ +++E++CT + + L+  R
Sbjct: 78  ELSGDFMKAVVSWTYDPAERDARLVNKILNKEKKKKSLENLKVIVEISCTTSPNHLIAVR 137

Query: 127 KAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLIKEA 186
           KAY SLFD S+EE +AS +  P  KLLV L S +RY+  +   E A  EA     +++EA
Sbjct: 138 KAYCSLFDSSLEEHIASSLPFPLAKLLVTLASTFRYDKDRTDAEVATIEAA----MLREA 197

Query: 187 ASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHG-----DLR--LQE 246
             KK   ++ + V+ IL TRS + L   +  YK+ + G +ID+D+ G     DLR  L+ 
Sbjct: 198 IEKKQ--LDHDHVLYILGTRSIYQLRETFVAYKK-NYGVTIDKDVDGCPGDADLRSLLKV 257

Query: 247 AVLCLANPVKYFTQVLNVSLK---VDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFGV 306
           A+ C+  P K+F +V+  S++    D D      LTR +VTRA+ D+ +++ E+ N +  
Sbjct: 258 AIFCIDTPEKHFAKVVRDSIEGFGTDEDS-----LTRAIVTRAEIDLMKVRGEYFNMYNT 317

Query: 307 SLAEKIESVCNGSYKDFLLTLL 315
           S+   I    +G YKDF++TLL
Sbjct: 318 SMDNAITGDISGDYKDFIITLL 317

BLAST of Lsi04G000400 vs. TAIR 10
Match: AT5G65020.1 (annexin 2 )

HSP 1 Score: 161.4 bits (407), Expect = 1.2e-39
Identity = 119/327 (36.39%), Postives = 179/327 (54.74%), Query Frame = 0

Query: 1   MADSAVEVLTRALSGHGINEKAMIDTLGKWDHEEKKLFRKRSSHFFSEDERSFERWQEHG 60
           + +   E L +A SG G NEK +I  L   +  ++ L R   +  ++ED           
Sbjct: 12  LPEDDAEQLHKAFSGWGTNEKLIISILAHRNAAQRSLIRSVYAATYNEDL---------- 71

Query: 61  MRLLKHEFMR-FKNAVVLWTTHPWERDARLVKEALSKGNHGQNINILIEVACTRTSDELL 120
           ++ L  E    F+ AV+LWT  P ERDA L KE  S     +N  +L+E+ACTR + EL+
Sbjct: 72  LKALDKELSSDFERAVMLWTLDPPERDAYLAKE--STKMFTKNNWVLVEIACTRPALELI 131

Query: 121 GARKAYHSLFDHSIEEDVASHINGPERKLLVALMSAYRYEGPKYKEETAKSEAKKLAHLI 180
             ++AY + +  SIEEDVA H +G  RKLL+ L+S +RYEG       A+SEAK    ++
Sbjct: 132 KVKQAYQARYKKSIEEDVAQHTSGDLRKLLLPLVSTFRYEGDDVNMMLARSEAK----IL 191

Query: 181 KEAASKKSSLIEDEEVVRILSTRSKHHLHALYKHYKEISAGRSIDEDLHGD------LRL 240
            E  S+KS    D++ +RIL+TRSK  L A   HY     G +I+++L  +      ++L
Sbjct: 192 HEKVSEKS--YSDDDFIRILTTRSKAQLGATLNHYNN-EYGNAINKNLKEESDDNDYMKL 251

Query: 241 QEAVL-CLANPVKYFTQVLNVSL-KVDADKKIKKVLTRIVVTRADKDMKEIKVEFKNQFG 300
             AV+ CL  P K+F +VL +S+ K+  D   +  LTR+V TR + DM+ IK E++ +  
Sbjct: 252 LRAVITCLTYPEKHFEKVLRLSINKMGTD---EWGLTRVVTTRTEVDMERIKEEYQRRNS 311

Query: 301 VSLAEKIESVCNGSYKDFLLTLLARSD 319
           + L   I    +G Y+D L+ LL   D
Sbjct: 312 IPLDRAIAKDTSGDYEDMLVALLGHGD 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVJ61.9e-8554.55Annexin D4 OS=Arabidopsis thaliana OX=3702 GN=ANN4 PE=1 SV=1[more]
P510746.0e-4437.04Annexin-like protein RJ4 OS=Fragaria ananassa OX=3747 PE=2 SV=2[more]
P931573.3e-4237.90Annexin Gh1 (Fragment) OS=Gossypium hirsutum OX=3635 GN=AnnGh1 PE=1 SV=2[more]
Q9SYT01.8e-4036.25Annexin D1 OS=Arabidopsis thaliana OX=3702 GN=ANN1 PE=1 SV=1[more]
Q94CK45.3e-4037.66Annexin D8 OS=Arabidopsis thaliana OX=3702 GN=ANNAT8 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KRR11.3e-16393.08Annexin 11 OS=Cucumis sativus OX=3659 GN=Csa_5G623670 PE=4 SV=1[more]
A0A5A7SZK16.7e-16392.45Annexin D4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002980 PE... [more]
E5GCK46.7e-16392.45Annexin OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3BH446.7e-16392.45annexin D4 OS=Cucumis melo OX=3656 GN=LOC103489495 PE=4 SV=1[more]
A0A6J1CB971.7e-15889.31annexin D4 OS=Momordica charantia OX=3673 GN=LOC111010078 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038891533.14.6e-16695.60annexin D4 [Benincasa hispida][more]
XP_004142407.12.8e-16393.08annexin D4 [Cucumis sativus] >KGN52303.1 hypothetical protein Csa_009205 [Cucumi... [more]
XP_008446930.11.4e-16292.45PREDICTED: annexin D4 [Cucumis melo] >ADN34201.1 annexin [Cucumis melo subsp. me... [more]
XP_022139070.13.5e-15889.31annexin D4 [Momordica charantia][more]
XP_022945018.16.0e-15890.25annexin D4-like [Cucurbita moschata] >KAG6573813.1 Annexin D4, partial [Cucurbit... [more]
Match NameE-valueIdentityDescription
AT2G38750.11.3e-8654.55annexin 4 [more]
AT1G35720.11.3e-4136.25annexin 1 [more]
AT5G12380.13.8e-4137.66annexin 8 [more]
AT2G38760.12.4e-4035.40annexin 3 [more]
AT5G65020.11.2e-3936.39annexin 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001464AnnexinPRINTSPR00196ANNEXINcoord: 14..36
score: 24.15
coord: 257..277
score: 26.46
coord: 180..206
score: 22.63
coord: 285..300
score: 34.72
IPR018502Annexin repeatSMARTSM00335annex3coord: 100..152
e-value: 2.5E-7
score: 40.3
coord: 191..240
e-value: 0.28
score: 6.3
coord: 264..313
e-value: 1.4E-7
score: 41.2
IPR018502Annexin repeatPFAMPF00191Annexincoord: 180..231
e-value: 1.0E-4
score: 22.3
coord: 264..313
e-value: 3.4E-10
score: 39.9
coord: 86..152
e-value: 1.4E-7
score: 31.4
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 166..242
score: 13.838779
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 81..154
score: 19.657957
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 166..241
e-value: 3.6E-10
score: 41.8
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 243..315
e-value: 1.1E-13
score: 53.0
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 81..153
e-value: 2.8E-15
score: 58.3
IPR037104Annexin superfamilySUPERFAMILY47874Annexincoord: 5..316
NoneNo IPR availablePANTHERPTHR10502:SF196ANNEXIN D4coord: 4..317
NoneNo IPR availablePANTHERPTHR10502ANNEXINcoord: 4..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000400.1Lsi04G000400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006950 response to stress
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005544 calcium-dependent phospholipid binding
molecular_function GO:0005509 calcium ion binding