Lsi04G000370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G000370
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Uroporphyrinogen decarboxylase
Location	chr04: 527026 .. 528438 (-)
RNA-Seq Expression	Lsi04G000370
Synteny	Lsi04G000370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGATGGTGATGAACTTACCATAAATGAAAACAAAAAGAAAATTGTTTACAGCAAAGAAACGTAAGGTTTAATAACGTGTTGCAGTCCACGAGTATGATGTTTCCTCTACGTGTTAATTAATTTGTCAATTCTCATTTTGTATTGAAAATTTGTTGTTTGTTATTTTTAAAGTTTATTTCACGTAATAGTCGTTGTGAAAAGTATAAATTGTTGAATATACAACTAATATAGATCGAGTTAGAAGATTTGAACCTTTGACCTTAAAGAAGACAGTGCATGTCAACTACTCATGAACTATACATACTTTCATTTATGATCGATCACTTTTGGTATGTTGAGAAAAAAAGAAAAGAAAATAAGATATTGTAATTTTCTAGTAATTCAACAAAAAAAAAGTATATGTGTAACGACCCGAGTTCATGAGGGGGACATGAATAAACCGAGATCACATCCAAATGAGAGAAAAATTTTCGTAAGATGCATGTGAGTGGGAACAATAGTTGCAAATATAGCAATAAAGTTTAAAGTATTAGTAGATATATATAGCAAAATTTAGATCCAACTATTAAAGTCAATTAGTGATAGACCCTAATAGCTTTATTAAGGATAGATATTTTATACACCTAATTATTACTTACCATTACCCAAATTAATTTCAATAACATTGGGTTGATTAAACGGATCCAAATTGGATAAGAAAATTAGCTGATGGTGTAATAAGATGGGCCTTGGGCTGGGCTTGCTTTCGTTCACAACCCGAGACCCAATCCCAAGATGGCCGTACACTCATGTGACAACGCTAGCCCTAGAAGCCATTGTGCTTTGCTCGACACTCAAGACACCGGCCTTCATATTCCGCCGCCAGCCTGCTGCCACTGCTCGCCGTCGACCGTTTTACATCGCCGACTACCATCCGGTATATGTTGTTTTTGCGTCACTATTTGTTACTAAAATTTTGTAGCTCTAGCCCGAGGAATTTGATCTGGTTTAAGCCTTGACATCCGAAAATACGATGAGTGGGTGAAGGGCAAGAACAGGGAGAAATATGAGGTTTTCGTGTGTAAAACAAGTTCTACATTTGAGAGGCTTGAGAGAATCCTTAAGTTGCCCATGGCTTTTTTCTGCCATTTCTTACTACTCAGATATTCCCTCAAAGAAGTCTAAGCTTGCCCCTTTACAGGTTTTTCACCTGCTTCTCTTCTATATTTTTCATTACTTTTTCACTATACAAATGCAAATTATTACACTCATGCTTTGGGATTATTGTGATCCTATGCAGGAGAGGAGAATGATAGACAGGTTTAAGGTGTACGCTAAAGGAGGTGATGGTGGAAATGGTTGCCATAGCATGCGGCGTAGTAGGCACGAACGCCACGGCCAACCTGATGGTACTTTTTATATGTCTAATTAA

mRNA sequence

AATGATGGTGATGAACTTACCATAAATGAAAACAAAAAGAAAATTGTTTACAGCAAAGAAACACACCGGCCTTCATATTCCGCCGCCAGCCTGCTGCCACTGCTCGCCGTCGACCGTTTTACATCGCCGACTACCATCCGGGCAAGAACAGGGAGAAATATGAGGTTTTCGTGTGTAAAACAAGTTCTACATTTGAGAGGCTTGAGAGAATCCTTAAGTTGCCCATGGCTTTTTTCTGCCATTTCTTACTACTCAGATATTCCCTCAAAGAAGTCTAAGCTTGCCCCTTTACAGGAGAGGAGAATGATAGACAGGTTTAAGGTGTACGCTAAAGGAGGTGATGGTGGAAATGGTTGCCATAGCATGCGGCGTAGTAGGCACGAACGCCACGGCCAACCTGATGGTACTTTTTATATGTCTAATTAA

Coding sequence (CDS)

AATGATGGTGATGAACTTACCATAAATGAAAACAAAAAGAAAATTGTTTACAGCAAAGAAACACACCGGCCTTCATATTCCGCCGCCAGCCTGCTGCCACTGCTCGCCGTCGACCGTTTTACATCGCCGACTACCATCCGGGCAAGAACAGGGAGAAATATGAGGTTTTCGTGTGTAAAACAAGTTCTACATTTGAGAGGCTTGAGAGAATCCTTAAGTTGCCCATGGCTTTTTTCTGCCATTTCTTACTACTCAGATATTCCCTCAAAGAAGTCTAAGCTTGCCCCTTTACAGGAGAGGAGAATGATAGACAGGTTTAAGGTGTACGCTAAAGGAGGTGATGGTGGAAATGGTTGCCATAGCATGCGGCGTAGTAGGCACGAACGCCACGGCCAACCTGATGGTACTTTTTATATGTCTAATTAA

Protein sequence

NDGDELTINENKKKIVYSKETHRPSYSAASLLPLLAVDRFTSPTTIRARTGRNMRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGGDGGNGCHSMRRSRHERHGQPDGTFYMSN

Homology

[Close]

BLAST of Lsi04G000370 vs. ExPASy Swiss-Prot
Match: F4HSD4 (Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ATOBGM PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.3e-16
Identity = 38/74 (51.35%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 62  VLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGGDGGNGCHS 121
           V +L   +     PW+ + + +YSD   KK K+APLQE RM DRF +YA+GG+GG+GC S
Sbjct: 10  VRYLGSYKRPQKPPWMRNPVVFYSDFSEKKGKVAPLQETRMRDRFTLYARGGEGGSGCSS 69

Query: 122 MRRSRHERHGQPDG 136
           +RRSR +R+G+PDG
Sbjct: 70  VRRSRADRYGKPDG 83

[Close]

BLAST of Lsi04G000370 vs. ExPASy Swiss-Prot
Match: Q2QZ37 (Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=OBGM PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.0e-12
Identity = 34/54 (62.96%), Postives = 42/54 (77.78%), Query Frame = 0


Query: 83  YYSDIP-SKKSKLAPLQERRMIDRFKVYAKGGDGGNGCHSMRRSRHERHGQPDG 136
           YY+  P  +K K APLQ R M+DRF++ AKGGDGGNGC S+RRSR +R G+PDG
Sbjct: 26  YYATEPEGRKPKTAPLQSRGMVDRFRLRAKGGDGGNGCISLRRSRSDRQGKPDG 79

[Close]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL
Match: A0A0A0KWW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 8.1e-36
Identity = 75/82 (91.46%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VLHLRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKRVLHLRGLRESSSCPWLFSAISYYSDTPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHERHG PDG
Sbjct: 61  DGGNGCQSMRRSRHERHGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL
Match: A0A6J1DCJ9 (probable GTP-binding protein OBGM, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111019488 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.0e-34
Identity = 73/82 (89.02%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK VL L+GLRES S PWLFSAISYYSD PSKKSKLAPLQERRM+DRFKVYAKGG
Sbjct: 1   MRFLCVKTVLRLKGLRESSSRPWLFSAISYYSDTPSKKSKLAPLQERRMVDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGCHSMRRSRH+RHGQPDG
Sbjct: 61  DGGNGCHSMRRSRHDRHGQPDG 82

[Close]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL
Match: A0A5D3CBS7 (Putative GTP-binding protein OBGM OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003000 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.4e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL
Match: E5GCK2 (Mitochondrial GTPase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.4e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL
Match: A0A1S3BG88 (probable GTP-binding protein OBGM, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489498 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.4e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. NCBI nr
Match: XP_004142409.1 (probable GTP-binding protein OBGM, mitochondrial [Cucumis sativus] >KGN52306.1 hypothetical protein Csa_008370 [Cucumis sativus])

HSP 1 Score: 159.8 bits (403), Expect = 1.7e-35
Identity = 75/82 (91.46%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VLHLRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKRVLHLRGLRESSSCPWLFSAISYYSDTPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHERHG PDG
Sbjct: 61  DGGNGCQSMRRSRHERHGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. NCBI nr
Match: XP_022151583.1 (probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_022151584.1 probable GTP-binding protein OBGM, mitochondrial [Momordica charantia])

HSP 1 Score: 155.2 bits (391), Expect = 4.1e-34
Identity = 73/82 (89.02%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK VL L+GLRES S PWLFSAISYYSD PSKKSKLAPLQERRM+DRFKVYAKGG
Sbjct: 1   MRFLCVKTVLRLKGLRESSSRPWLFSAISYYSDTPSKKSKLAPLQERRMVDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGCHSMRRSRH+RHGQPDG
Sbjct: 61  DGGNGCHSMRRSRHDRHGQPDG 82

[Close]

BLAST of Lsi04G000370 vs. NCBI nr
Match: XP_008446934.1 (PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 154.5 bits (389), Expect = 7.0e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. NCBI nr
Match: ADN34199.1 (mitochondrial GTPase [Cucumis melo subsp. melo])

HSP 1 Score: 154.5 bits (389), Expect = 7.0e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. NCBI nr
Match: XP_008446933.1 (PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X1 [Cucumis melo] >KAA0034749.1 putative GTP-binding protein OBGM [Cucumis melo var. makuwa] >TYK09303.1 putative GTP-binding protein OBGM [Cucumis melo var. makuwa])

HSP 1 Score: 154.5 bits (389), Expect = 7.0e-34
Identity = 73/82 (89.02%), Postives = 75/82 (91.46%), Query Frame = 0


Query: 54  MRFSCVKQVLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGG 113
           MRF CVK+VL LRGLRES SCPWLFSAISYYSD P KKSKLAPLQERRMIDRFKVYAKGG
Sbjct: 1   MRFLCVKKVLRLRGLRESSSCPWLFSAISYYSDAPKKKSKLAPLQERRMIDRFKVYAKGG 60

Query: 114 DGGNGCHSMRRSRHERHGQPDG 136
           DGGNGC SMRRSRHER+G PDG
Sbjct: 61  DGGNGCQSMRRSRHERYGHPDG 82

[Close]

BLAST of Lsi04G000370 vs. TAIR 10
Match: AT1G07615.1 (GTP-binding protein Obg/CgtA )

HSP 1 Score: 85.5 bits (210), Expect = 3.7e-17
Identity = 38/74 (51.35%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 62  VLHLRGLRESLSCPWLFSAISYYSDIPSKKSKLAPLQERRMIDRFKVYAKGGDGGNGCHS 121
           V +L   +     PW+ + + +YSD   KK K+APLQE RM DRF +YA+GG+GG+GC S
Sbjct: 10  VRYLGSYKRPQKPPWMRNPVVFYSDFSEKKGKVAPLQETRMRDRFTLYARGGEGGSGCSS 69

Query: 122 MRRSRHERHGQPDG 136
           +RRSR +R+G+PDG
Sbjct: 70  VRRSRADRYGKPDG 83

The following BLAST results are available for this feature:

BLAST of Lsi04G000370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HSD4	5.3e-16	51.35	Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
Q2QZ37	6.0e-12	62.96	Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica...	[more]

BLAST of Lsi04G000370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWW4	8.1e-36	91.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1	[more]
A0A6J1DCJ9	2.0e-34	89.02	probable GTP-binding protein OBGM, mitochondrial OS=Momordica charantia OX=3673 ...	[more]
A0A5D3CBS7	3.4e-34	89.02	Putative GTP-binding protein OBGM OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
E5GCK2	3.4e-34	89.02	Mitochondrial GTPase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3BG88	3.4e-34	89.02	probable GTP-binding protein OBGM, mitochondrial isoform X1 OS=Cucumis melo OX=3...	[more]

BLAST of Lsi04G000370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142409.1	1.7e-35	91.46	probable GTP-binding protein OBGM, mitochondrial [Cucumis sativus] >KGN52306.1 h...	[more]
XP_022151583.1	4.1e-34	89.02	probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_02215...	[more]
XP_008446934.1	7.0e-34	89.02	PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X2 [Cucumis ...	[more]
ADN34199.1	7.0e-34	89.02	mitochondrial GTPase [Cucumis melo subsp. melo]	[more]
XP_008446933.1	7.0e-34	89.02	PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X1 [Cucumis ...	[more]

BLAST of Lsi04G000370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07615.1	3.7e-17	51.35	GTP-binding protein Obg/CgtA	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036726	GTP1/OBG domain superfamily	GENE3D	2.70.210.12	GTP1/OBG domain	coord: 98..137 e-value: 1.0E-6 score: 30.4
IPR036726	GTP1/OBG domain superfamily	SUPERFAMILY	82051	Obg GTP-binding protein N-terminal domain	coord: 102..135
IPR006169	GTP1/OBG domain	PFAM	PF01018	GTP1_OBG	coord: 103..135 e-value: 7.3E-7 score: 29.0
IPR006169	GTP1/OBG domain	PROSITE	PS51883	OBG	coord: 100..141 score: 12.942591
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 117..141
None	No IPR available	PANTHER	PTHR11702:SF42	BNAA08G29050D PROTEIN	coord: 80..136
IPR045086	OBG-type GTPase	PANTHER	PTHR11702	DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED	coord: 80..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G000370.1	Lsi04G000370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042254	ribosome biogenesis
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0000287	magnesium ion binding