Lsi04G000360 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G000360
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMitochondrial GTPase
Locationchr04: 524524 .. 526994 (-)
RNA-Seq ExpressionLsi04G000360
SyntenyLsi04G000360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAAGGTAGATTAAAACATTCCCCTCAGCAACTATATTTTAAACCAATTATCTTCTAATATAAACAAATGCACATTTGATGGCATTTCTTTTAAGCTTCCTAAACTACTGCATTATTCAGTTTTGGACAGAGTTTACTTGTTAGTTATTGAACACACACACACACGCACATATTTTAATATCATGAGTGTTCGAGCTAGGTGAATCTAGAACCAATTGACTTCACATTGCTGTTCTTGAATGCTGCCTTTCTAATTGATTCAGGTGGAAATGGTGGAAGAGGGGGCGACGTGATTCTGGAATGTTCCACTGCTCTATGGGACTTCAGCAGTTTGAAGCATCATATTGTATGTTAGACTCTTCTTTATGCTCCTGCTAGGAACATATCGAAAATGAATGATAAACTATCTTTACGGTTACAGTCTTACAGAATTACGACATCTATCAATATTCCTACCATCTCTTGCAGAATGCAAGTAGAGGAGGACATGGATCTTCGAAAAATAAGATTGGAACTAAAGGTGCAGATAAGGTCATCTATCTCCTCCTGATCTGTATGTGTGCTGTCGTTGCTGCTTTTTTTTGTTTCTTAATTGTTCATATTTTTTATTTAAACTACAAATTTAATTCCTAATTTTGGCAGCTATCTGTTCCATCTCTAAATTTTATTTAGTTTCAATTGTGTTCCTAAATTTTGAATTATGATTGTATACGTAGTTCTTGATGTGTTTATCGAACAAATATTAGCTAATTTGACACAATGGTGTGTAGACCAATTGCAGAATGAAGTAGAAGGTTGGATAAACGACAGTGATATAGTTTACAAAAAGATTTTGAGATGTTGCTTAAAAAAGGATAGGTTTATACTGTCCTACCCTACCGTCTATCTATTTTGTCATACTAAATCGGTCAGAAACAAGTTAGTCATCTTTTAGATAATACTTAATAGTGCTAGTAATAGGAATTATAGTTCAAAATTTATACATGTGATTGAAATTTTGTATAGTTTTAGAGAGTAAATAGATTCTGCAATCAAATTTAGGGGCTAAATTTGTAGTGCAAACAATATTTTTTCTTTTGGTTATTAAAATTATTAGGATCCAACTCTACTGAGTCATATATTTGAATCATAGATTGTTCAAGTACCTATTGGCACTGTCATTCATCTTGTGGAGGGTGAAGTTCCCAGTGTAGTCAAACATCATTCTTCAACAGATTTAGATCCTTGGCAGATTCCTGGCACACTAGTTGATGATCTCTCTAGTTCCCACCAGAAGTCTCTTAAATATTCAAACAGTGAGAAGGAAGTTGAATCGACATTTATGACTACTCTCTTGCCGTGTAATAATAGTAAAAATAATGTTAAAGATTCGTCGTTCCGGAGGGAAACTTCTGAAGTTACATCAACTGATGGGATTTCTCAATTTTCTGCATATCGTGAGAGTTCTATTCAAGATGAGATTGGAGAAAGTGAAGAAATGACGTACAATGTTGCAGAATTGACTGAAGAAGGTCAGCGAATTATCATTGCTCGTGGAGGGGAAGGTGGTTTGGGCAATATTCATGAACTTAAAGTTTCAAAGAAGCCTAAAGCCTCAATACCTATGGGGCAAGAGGATGAGTCCATAGATTCTAATGATAACGAGTCCAACAGGCGAGCTGGATCTCATGGTAGCGAGGCTGTGCTTGTTTTAGAACTTAAAAGCATTGCGGACGTGGGTTTCGTTGGAATGCCAAATGCTGGGAAAAGTACTTTGCTTGGAGCCATTTCCAGAGCAAAACCAGTAGTTGGTCATTATGCATTCACAACTCTGAGGCCGAATTTGGGAAACCTGCATTATGACGACTTATCAATCACAGTAGCTGACATTCCAGGTCTTATAAAGGGTGCGCACGAAAATCGTGGACTTGGACATTCTTTCTTGCGTCACATCGAACGCACAAGGGTCCTAGCATATGTCGTAGACTTGGCTGCTACTTTAGATGGTAGAAAAGGGAAACCCCCATGGGAACAACTGAGAGATTTAGTTTCAGAGCTTGAACGCCATCAGAATGGGTTATCAGATCGGCCATCCTTAATAGTGGCTAACAAGATGGACGAAGAAGGGGCCGAGGAAATGTATGAAGAGTTAAAGTGTAGAGTGCAAGGAGTTCTCATCTTTCCCGTTTGTGCCGTTTTGGGAGAGGGTGTTGAGGACCTTAAAGCTGGTCTTAAGAGCCTCGTGAATGGTGATACATCTTATAAGCTTAAGGTAGATGATATTATTGTTCATTAGGTGTTTGAAATGTACCATTTCATTCCATTTTTGCTGTTGTGACTGATAGAACTTTAGAGATTATTGATGAAGAATAGATAGATATATTTATGAAATTTCATCAAGAGACCCCAGGATTTTGTATAAATTAATAAAAAATTATGAATCTGATCATTATAAATAAAATGTTAAAATACCATTTTTGTTCTTCTA

mRNA sequence

ATGGGCAAGGTGAATCTAGAACCAATTGACTTCACATTGCTGTTCTTGAATGCTGCCTTTCTAATTGATTCAGGTGGAAATGGTGGAAGAGGGGGCGACGTGATTCTGGAATGTTCCACTGCTCTATGGGACTTCAGCAGTTTGAAGCATCATATTAATGCAAGTAGAGGAGGACATGGATCTTCGAAAAATAAGATTGGAACTAAAGGTGCAGATAAGATTGTTCAAGTACCTATTGGCACTGTCATTCATCTTGTGGAGGGTGAAGTTCCCAGTGTAGTCAAACATCATTCTTCAACAGATTTAGATCCTTGGCAGATTCCTGGCACACTAGTTGATGATCTCTCTAGTTCCCACCAGAAGTCTCTTAAATATTCAAACAGTGAGAAGGAAGTTGAATCGACATTTATGACTACTCTCTTGCCGTGTAATAATAGTAAAAATAATGTTAAAGATTCGTCGTTCCGGAGGGAAACTTCTGAAGTTACATCAACTGATGGGATTTCTCAATTTTCTGCATATCGTGAGAGTTCTATTCAAGATGAGATTGGAGAAAGTGAAGAAATGACGTACAATGTTGCAGAATTGACTGAAGAAGGTCAGCGAATTATCATTGCTCGTGGAGGGGAAGGTGGTTTGGGCAATATTCATGAACTTAAAGTTTCAAAGAAGCCTAAAGCCTCAATACCTATGGGGCAAGAGGATGAGTCCATAGATTCTAATGATAACGAGTCCAACAGGCGAGCTGGATCTCATGGTAGCGAGGCTGTGCTTGTTTTAGAACTTAAAAGCATTGCGGACGTGGGTTTCGTTGGAATGCCAAATGCTGGGAAAAGTACTTTGCTTGGAGCCATTTCCAGAGCAAAACCAGTAGTTGGTCATTATGCATTCACAACTCTGAGGCCGAATTTGGGAAACCTGCATTATGACGACTTATCAATCACAGTAGCTGACATTCCAGGTCTTATAAAGGGTGCGCACGAAAATCGTGGACTTGGACATTCTTTCTTGCGTCACATCGAACGCACAAGGGTCCTAGCATATGTCGTAGACTTGGCTGCTACTTTAGATGGTAGAAAAGGGAAACCCCCATGGGAACAACTGAGAGATTTAGTTTCAGAGCTTGAACGCCATCAGAATGGGTTATCAGATCGGCCATCCTTAATAGTGGCTAACAAGATGGACGAAGAAGGGGCCGAGGAAATGTATGAAGAGTTAAAGTGTAGAGTGCAAGGAGTTCTCATCTTTCCCGTTTGTGCCGTTTTGGGAGAGGGTGTTGAGGACCTTAAAGCTGGTCTTAAGAGCCTCGTGAATGGTGATACATCTTATAAGCTTAAGGTAGATGATATTATTGTTCATTAGGTGTTTGAAATGTACCATTTCATTCCATTTTTGCTGTTGTGACTGATAGAACTTTAGAGATTATTGATGAAGAATAGATAGATATATTTATGAAATTTCATCAAGAGACCCCAGGATTTTGTATAAATTAATAAAAAATTATGAATCTGATCATTATAAATAAAATGTTAAAATACCATTTTTGTTCTTCTA

Coding sequence (CDS)

ATGGGCAAGGTGAATCTAGAACCAATTGACTTCACATTGCTGTTCTTGAATGCTGCCTTTCTAATTGATTCAGGTGGAAATGGTGGAAGAGGGGGCGACGTGATTCTGGAATGTTCCACTGCTCTATGGGACTTCAGCAGTTTGAAGCATCATATTAATGCAAGTAGAGGAGGACATGGATCTTCGAAAAATAAGATTGGAACTAAAGGTGCAGATAAGATTGTTCAAGTACCTATTGGCACTGTCATTCATCTTGTGGAGGGTGAAGTTCCCAGTGTAGTCAAACATCATTCTTCAACAGATTTAGATCCTTGGCAGATTCCTGGCACACTAGTTGATGATCTCTCTAGTTCCCACCAGAAGTCTCTTAAATATTCAAACAGTGAGAAGGAAGTTGAATCGACATTTATGACTACTCTCTTGCCGTGTAATAATAGTAAAAATAATGTTAAAGATTCGTCGTTCCGGAGGGAAACTTCTGAAGTTACATCAACTGATGGGATTTCTCAATTTTCTGCATATCGTGAGAGTTCTATTCAAGATGAGATTGGAGAAAGTGAAGAAATGACGTACAATGTTGCAGAATTGACTGAAGAAGGTCAGCGAATTATCATTGCTCGTGGAGGGGAAGGTGGTTTGGGCAATATTCATGAACTTAAAGTTTCAAAGAAGCCTAAAGCCTCAATACCTATGGGGCAAGAGGATGAGTCCATAGATTCTAATGATAACGAGTCCAACAGGCGAGCTGGATCTCATGGTAGCGAGGCTGTGCTTGTTTTAGAACTTAAAAGCATTGCGGACGTGGGTTTCGTTGGAATGCCAAATGCTGGGAAAAGTACTTTGCTTGGAGCCATTTCCAGAGCAAAACCAGTAGTTGGTCATTATGCATTCACAACTCTGAGGCCGAATTTGGGAAACCTGCATTATGACGACTTATCAATCACAGTAGCTGACATTCCAGGTCTTATAAAGGGTGCGCACGAAAATCGTGGACTTGGACATTCTTTCTTGCGTCACATCGAACGCACAAGGGTCCTAGCATATGTCGTAGACTTGGCTGCTACTTTAGATGGTAGAAAAGGGAAACCCCCATGGGAACAACTGAGAGATTTAGTTTCAGAGCTTGAACGCCATCAGAATGGGTTATCAGATCGGCCATCCTTAATAGTGGCTAACAAGATGGACGAAGAAGGGGCCGAGGAAATGTATGAAGAGTTAAAGTGTAGAGTGCAAGGAGTTCTCATCTTTCCCGTTTGTGCCGTTTTGGGAGAGGGTGTTGAGGACCTTAAAGCTGGTCTTAAGAGCCTCGTGAATGGTGATACATCTTATAAGCTTAAGGTAGATGATATTATTGTTCATTAG

Protein sequence

MGKVNLEPIDFTLLFLNAAFLIDSGGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIHLVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCNNSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRIIIARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNESNRRAGSHGSEAVLVLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDTSYKLKVDDIIVH
Homology
BLAST of Lsi04G000360 vs. ExPASy Swiss-Prot
Match: F4HSD4 (Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ATOBGM PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.2e-134
Identity = 253/437 (57.89%), Postives = 319/437 (73.00%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GGNGGRGGDVILEC+ A+WDFS L+ HI   + GHG+SKN+IG +G DK++ VPIGTVIH
Sbjct: 83  GGNGGRGGDVILECTHAVWDFSGLQPHIKGGKAGHGTSKNRIGNRGEDKVLLVPIGTVIH 142

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           L EGE+PS V++ S  DLDPW +PG+LV+D                           P +
Sbjct: 143 LQEGEIPSQVENESPKDLDPWDLPGSLVED---------------------------PAS 202

Query: 145 NSKNNVKDSSFRRETSEVTSTDGIS-QFSAYRESSIQDEIGESEEMTYNVAELTEEGQRI 204
              ++V   +      + T  + ++ Q    +E+  +D+  E +++ YNVAELT++GQR+
Sbjct: 203 EENSDVHQETMSESDQDDTEQESLTRQLGMPKEADFEDDDEEIDQIRYNVAELTQQGQRV 262

Query: 205 IIARGGEGGLGNI---HELKVSKKPKASI---PMGQEDESIDSNDNESNRRAGSHGSEAV 264
           IIARGGEGGLGN+     ++ SK  K++I    +   ++  + +D  S+ +AG  GSEAV
Sbjct: 263 IIARGGEGGLGNVSATRYVRGSKFAKSTIRQTNLRSMEDDAEEDDERSSIKAGLLGSEAV 322

Query: 265 LVLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVA 324
           L+LELKSIADVG VGMPNAGKSTLLGA+SRAKP VGHYAFTTLRPNLGN++YDD S+TVA
Sbjct: 323 LILELKSIADVGLVGMPNAGKSTLLGALSRAKPRVGHYAFTTLRPNLGNVNYDDFSMTVA 382

Query: 325 DIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELER 384
           DIPGLIKGAH+NRGLGH+FLRHIERT+VLAYVVDLA+ LDG +G  PW+QLRDLV ELE 
Sbjct: 383 DIPGLIKGAHQNRGLGHNFLRHIERTKVLAYVVDLASGLDGCEGLTPWQQLRDLVMELEF 442

Query: 385 HQNGLSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLV 444
           H+ GLSDR SLIVANK+DEEGAEE  +ELK RV+GV IFPVCAVL EGV +LK GLK LV
Sbjct: 443 HEEGLSDRSSLIVANKIDEEGAEERLKELKRRVKGVKIFPVCAVLEEGVAELKDGLKMLV 492

Query: 445 --NGDTSYKLKVDDIIV 453
             NG+ S +LK+++I V
Sbjct: 503 DGNGEPSERLKLENICV 492

BLAST of Lsi04G000360 vs. ExPASy Swiss-Prot
Match: Q2QZ37 (Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=OBGM PE=2 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 4.2e-123
Identity = 247/460 (53.70%), Postives = 307/460 (66.74%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GGNGGRGGDVILECS ++WDFS L+HH+ ASRG +G SKN+IGT+G+DKI QVP+GTVIH
Sbjct: 79  GGNGGRGGDVILECSRSVWDFSGLQHHMKASRGANGVSKNQIGTRGSDKIAQVPVGTVIH 138

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDD------LSSSHQKSLKYSNSEKEVESTFMT 144
           LVEGE PS+  +  +  LDPW IP  +         + S   K L  S S + + S   T
Sbjct: 139 LVEGEQPSLSVNKPTRALDPWDIPDAVEHSPFSSSRIGSKMMKGLDSSRSSQHISSKKNT 198

Query: 145 TLLPC--NNSKNNVKDSSFRRETSEVTSTDGIS-----------QFSAYRE--------- 204
               C   N  +  K+  +  E       DG S           QF    +         
Sbjct: 199 AENDCERGNRNHRGKEPYYMTEFVRTEDYDGTSYPHQVGVDENDQFDDEDDEFWEDDEEE 258

Query: 205 ---SSIQDEIGESEEMTYNVAELTEEGQRIIIARGGEGGLGNIHELKVSKKPKASIPMGQ 264
                + +E  E E++ Y+VAE+T+ GQR+IIARGGEGGLGN   LK     KA     +
Sbjct: 259 LDMEEVTEEKEEEEDVRYSVAEMTKPGQRLIIARGGEGGLGNACILKEMWLSKAH----K 318

Query: 265 EDESIDSNDNESNRRAGSHGSEAVLVLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVG 324
           E+E        ++   G  G+E  L+LELKSIADVG VGMPNAGKSTLL A+SRA+P + 
Sbjct: 319 EEEM-------ASLSTGHPGTETYLILELKSIADVGLVGMPNAGKSTLLSALSRARPEIA 378

Query: 325 HYAFTTLRPNLGNLHYDD-LSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDL 384
            YAFTTLRPN+G+L Y+D  S+ VADIPGLIKGAHENRGLGH+FLRHIERT+VLAYV+DL
Sbjct: 379 DYAFTTLRPNIGSLTYEDYFSVKVADIPGLIKGAHENRGLGHAFLRHIERTKVLAYVLDL 438

Query: 385 AATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPSLIVANKMDEEGAEEMYEELKCRVQG 444
           AATL+GRKG PPWEQLRDLV ELE +Q GL+ RPSLIVANK+DEEGA+EMYEELK RVQG
Sbjct: 439 AATLNGRKGVPPWEQLRDLVVELEHYQEGLTKRPSLIVANKIDEEGADEMYEELKKRVQG 498

Query: 445 VLIFPVCAVLGEGVEDLKAGLKSLVNGDTSYKLKVDDIIV 453
           V +FP+CA+L EGV DL+ GL+ L++      +++  I+V
Sbjct: 499 VPMFPICAILQEGVPDLRVGLRDLMDASDPQGIELSKIVV 527

BLAST of Lsi04G000360 vs. ExPASy Swiss-Prot
Match: B2UQ30 (GTPase Obg OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=obg PE=3 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 6.8e-49
Identity = 115/270 (42.59%), Postives = 155/270 (57.41%), Query Frame = 0

Query: 169 SQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRIIIARGGEGGLGNIHELKVSKKPKAS 228
           S  S+  E++  +  GE  E+   +A+LTE G R  + +GG GG GN H    + +    
Sbjct: 98  SNASSMAEATWLEREGEGIELE-KIADLTEIGTRFTLCQGGLGGKGNWHFRSATNQAPTE 157

Query: 229 IPMGQEDESIDSNDNESNRRAGSHGSEAVLVLELKSIADVGFVGMPNAGKSTLLGAISRA 288
             MG E                  G E V  +EL+ IAD G VG PNAGKSTLLG IS A
Sbjct: 158 AEMGTE------------------GEEGVFFMELRRIADAGLVGYPNAGKSTLLGDISEA 217

Query: 289 KPVVGHYAFTTLRPNLGNLHYDDL-SITVADIPGLIKGAHENRGLGHSFLRHIERTRVLA 348
           KP V  Y FTTL+P +G + +D      VADIPG+I+GAH NRGLGH FLRHI R +VL 
Sbjct: 218 KPKVASYPFTTLQPIIGVVEFDSFRRCVVADIPGIIEGAHNNRGLGHEFLRHITRCKVLV 277

Query: 349 YVVDLAATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPSLIVANKMDEEGAEEMYEELK 408
           +V+D+A    G +G+ P E L++L +E++ +   L+ +P  +VANKMD EGAEE     +
Sbjct: 278 FVLDMA----GSEGRDPIEDLQNLRTEIKLYSEDLAKQPWFVVANKMDLEGAEENLANFR 337

Query: 409 CRVQGVLIFPVCAVLGEGVEDLKAGLKSLV 438
            R   V I P+ A+ G+G+  L+  L  LV
Sbjct: 338 MRFPKVDIIPISALNGDGISRLRNRLDELV 344

BLAST of Lsi04G000360 vs. ExPASy Swiss-Prot
Match: B8FUR9 (GTPase Obg OS=Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) OX=272564 GN=obg PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 144/413 (34.87%), Postives = 188/413 (45.52%), Query Frame = 0

Query: 24  SGGNGGRGGDVIL---ECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIG 83
           SGG+GGRGG VI    E    L DF   K H  A RG HG  KN  G  G D  V++P+G
Sbjct: 34  SGGDGGRGGKVIFIADEGLRTLVDF-RYKRHYKADRGEHGQGKNMHGKSGEDMSVRIPVG 93

Query: 84  TVIHLVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTL 143
           TV                                                          
Sbjct: 94  TV---------------------------------------------------------- 153

Query: 144 LPCNNSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEG 203
                    VKD+    +T E+                              +A+L E G
Sbjct: 154 ---------VKDA----DTGEI------------------------------LADLIEHG 213

Query: 204 QRIIIARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNESNRRA--GSHGSEAVL 263
           Q++++A GG GG GN   +                    SN N++   A  G  G E  L
Sbjct: 214 QKVVVANGGRGGRGNARFM--------------------SNTNKAPTVAENGEPGEERNL 273

Query: 264 VLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDD-LSITVA 323
           +LELK +ADVG VG PN GKST++  IS AKP +  Y FTTL PNLG +  +D  S  VA
Sbjct: 274 LLELKLLADVGLVGFPNVGKSTIISRISAAKPKIADYHFTTLVPNLGVVELEDGESFVVA 320

Query: 324 DIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELER 383
           DIPGLI+GAH   GLGH FLRH ERTR++ +V+D+A    G + + P E  + +  EL +
Sbjct: 334 DIPGLIEGAHTGAGLGHEFLRHTERTRLILHVLDIA----GSEERDPLEDFQIIAEELRQ 320

Query: 384 HQNGLSDRPSLIVANKMDEEGAEEMYEELKCRV-QGVLIFPVCAVLGEGVEDL 430
           +   L++RP LIVANKMD  GAEE  + L  ++ +   IFPV A  GEG+++L
Sbjct: 394 YSQELANRPILIVANKMDIPGAEENLQRLTEKLGEDYRIFPVSAATGEGLKEL 320

BLAST of Lsi04G000360 vs. ExPASy Swiss-Prot
Match: Q24SP0 (GTPase Obg OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=obg PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.6e-48
Identity = 144/413 (34.87%), Postives = 188/413 (45.52%), Query Frame = 0

Query: 24  SGGNGGRGGDVIL---ECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIG 83
           SGG+GGRGG VI    E    L DF   K H  A RG HG  KN  G  G D  V++P+G
Sbjct: 34  SGGDGGRGGKVIFIADEGLRTLVDF-RYKRHYKADRGEHGQGKNMHGKSGEDMSVRIPVG 93

Query: 84  TVIHLVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTL 143
           TV                                                          
Sbjct: 94  TV---------------------------------------------------------- 153

Query: 144 LPCNNSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEG 203
                    VKD+    +T E+                              +A+L E G
Sbjct: 154 ---------VKDA----DTGEI------------------------------LADLIEHG 213

Query: 204 QRIIIARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNESNRRA--GSHGSEAVL 263
           Q++++A GG GG GN   +                    SN N++   A  G  G E  L
Sbjct: 214 QKVVVANGGRGGRGNARFM--------------------SNTNKAPTVAENGEPGEERNL 273

Query: 264 VLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDD-LSITVA 323
           +LELK +ADVG VG PN GKST++  IS AKP +  Y FTTL PNLG +  +D  S  VA
Sbjct: 274 LLELKLLADVGLVGFPNVGKSTIISRISAAKPKIADYHFTTLVPNLGVVELEDGESFVVA 320

Query: 324 DIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELER 383
           DIPGLI+GAH   GLGH FLRH ERTR++ +V+D+A    G + + P E  + +  EL +
Sbjct: 334 DIPGLIEGAHTGAGLGHEFLRHTERTRLIFHVLDIA----GSEERDPLEDFQIIAEELRQ 320

Query: 384 HQNGLSDRPSLIVANKMDEEGAEEMYEELKCRV-QGVLIFPVCAVLGEGVEDL 430
           +   L++RP LIVANKMD  GAEE  + L  ++ +   IFPV A  GEG+++L
Sbjct: 394 YSQELANRPILIVANKMDIPGAEENLQRLTEKLGEDYRIFPVSAATGEGLKEL 320

BLAST of Lsi04G000360 vs. ExPASy TrEMBL
Match: A0A5D3CBS7 (Putative GTP-binding protein OBGM OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003000 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.4e-206
Identity = 372/432 (86.11%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHIKASRGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 511

BLAST of Lsi04G000360 vs. ExPASy TrEMBL
Match: A0A1S3BG88 (probable GTP-binding protein OBGM, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489498 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.4e-206
Identity = 372/432 (86.11%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHIKASRGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 511

BLAST of Lsi04G000360 vs. ExPASy TrEMBL
Match: A0A0A0KWW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.4e-206
Identity = 371/432 (85.88%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFSSL HHINAS+GGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSSLNHHINASKGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVV+HHSSTDLDPWQIPGTLVDDLSS H+ S K+SN E EVES F TTL+ CN
Sbjct: 142 LVEGEVPSVVEHHSSTDLDPWQIPGTLVDDLSSHHKNSFKFSNRETEVESAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
            SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQRII
Sbjct: 202 ESKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQRII 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSN---DNESNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK+S+  G ED+SIDSN    NESNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKSSV--GHEDKSIDSNLSEINESNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP +GHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTIGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGV IFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVPIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 511

BLAST of Lsi04G000360 vs. ExPASy TrEMBL
Match: A0A1S3BGW6 (probable GTP-binding protein OBGM, mitochondrial isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489498 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.5e-205
Identity = 372/432 (86.11%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHI-ASRGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 510

BLAST of Lsi04G000360 vs. ExPASy TrEMBL
Match: E5GCK2 (Mitochondrial GTPase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.1e-194
Identity = 356/430 (82.79%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADK+V + I     
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHIKASRGGHGSSKNKIGTKGADKVVYLLI----- 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
                 PSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 -----FPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 499

Query: 445 SYKLKVDDII 452
             +LK+D+II
Sbjct: 502 PSRLKIDEII 499

BLAST of Lsi04G000360 vs. NCBI nr
Match: XP_008446933.1 (PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X1 [Cucumis melo] >KAA0034749.1 putative GTP-binding protein OBGM [Cucumis melo var. makuwa] >TYK09303.1 putative GTP-binding protein OBGM [Cucumis melo var. makuwa])

HSP 1 Score: 728.8 bits (1880), Expect = 2.9e-206
Identity = 372/432 (86.11%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHIKASRGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 511

BLAST of Lsi04G000360 vs. NCBI nr
Match: XP_004142409.1 (probable GTP-binding protein OBGM, mitochondrial [Cucumis sativus] >KGN52306.1 hypothetical protein Csa_008370 [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 4.9e-206
Identity = 371/432 (85.88%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFSSL HHINAS+GGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSSLNHHINASKGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVV+HHSSTDLDPWQIPGTLVDDLSS H+ S K+SN E EVES F TTL+ CN
Sbjct: 142 LVEGEVPSVVEHHSSTDLDPWQIPGTLVDDLSSHHKNSFKFSNRETEVESAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
            SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQRII
Sbjct: 202 ESKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQRII 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSN---DNESNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK+S+  G ED+SIDSN    NESNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKSSV--GHEDKSIDSNLSEINESNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP +GHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTIGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGV IFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVPIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 511

BLAST of Lsi04G000360 vs. NCBI nr
Match: XP_008446934.1 (PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 724.2 bits (1868), Expect = 7.1e-205
Identity = 372/432 (86.11%), Postives = 397/432 (91.90%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADKIV+VPIGTVIH
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHI-ASRGGHGSSKNKIGTKGADKIVRVPIGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 501

Query: 445 SYKLKVDDIIVH 454
             +LK+D+IIVH
Sbjct: 502 PSRLKIDEIIVH 510

BLAST of Lsi04G000360 vs. NCBI nr
Match: ADN34199.1 (mitochondrial GTPase [Cucumis melo subsp. melo])

HSP 1 Score: 688.3 bits (1775), Expect = 4.3e-194
Identity = 356/430 (82.79%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GG+GGRGGDVILECSTALWDFS+L HHI ASRGGHGSSKNKIGTKGADK+V + I     
Sbjct: 82  GGDGGRGGDVILECSTALWDFSTLNHHIKASRGGHGSSKNKIGTKGADKVVYLLI----- 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
                 PSVVKHHSSTDLDPWQIPGTLVDDLSSSHQ S K+SN E EV S F TTL+ CN
Sbjct: 142 -----FPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQMSFKFSNRETEVNSAFKTTLVTCN 201

Query: 145 NSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQRII 204
           +SKNNV++SSFRRETSEV STD ISQ SA+ +SSIQDE GESEEM YNVAELTEEGQ++I
Sbjct: 202 DSKNNVRNSSFRRETSEVASTDEISQVSAFPDSSIQDEFGESEEMMYNVAELTEEGQQVI 261

Query: 205 IARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNE---SNRRAGSHGSEAVLVLE 264
           IARGGEGGLGN+HE K+SKKPK S+  G EDESIDSN +E   SNRR GS GSEAVLVLE
Sbjct: 262 IARGGEGGLGNVHEHKLSKKPKTSV--GHEDESIDSNLSEINVSNRRTGSLGSEAVLVLE 321

Query: 265 LKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPG 324
           LKSIADVGFVGMPNAGKSTLLGAISRAKP VGHYAFTTLRPNLGNLHYDDLSITVADIPG
Sbjct: 322 LKSIADVGFVGMPNAGKSTLLGAISRAKPTVGHYAFTTLRPNLGNLHYDDLSITVADIPG 381

Query: 325 LIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNG 384
           LIKGAHENRGLGHSFLRHIERTRVLAYV+DLAA LDGRKG PPWEQLRDLV ELERHQ+G
Sbjct: 382 LIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAALDGRKGIPPWEQLRDLVYELERHQSG 441

Query: 385 LSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVNGDT 444
           LSDRPSLIVANK+DEEGAEE+YEELK RVQGVLIFPVCAVL EGV++LKAGLKSLVNGDT
Sbjct: 442 LSDRPSLIVANKIDEEGAEEVYEELKSRVQGVLIFPVCAVLEEGVDELKAGLKSLVNGDT 499

Query: 445 SYKLKVDDII 452
             +LK+D+II
Sbjct: 502 PSRLKIDEII 499

BLAST of Lsi04G000360 vs. NCBI nr
Match: XP_022151583.1 (probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_022151584.1 probable GTP-binding protein OBGM, mitochondrial [Momordica charantia])

HSP 1 Score: 682.6 bits (1760), Expect = 2.4e-192
Identity = 356/434 (82.03%), Postives = 384/434 (88.48%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GGNGGRGGDVILECS +LWDFSSL HHINASRGGHGSSK KIGT+G DKIVQVP+GTVIH
Sbjct: 82  GGNGGRGGDVILECSPSLWDFSSLNHHINASRGGHGSSKKKIGTRGEDKIVQVPVGTVIH 141

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           LVEGE+PSVVK HSSTDLDPWQIPGTLV+D+S S Q SLKYSN E+EVES   TTLLPCN
Sbjct: 142 LVEGELPSVVKQHSSTDLDPWQIPGTLVNDISDSRQTSLKYSNKEEEVESIVKTTLLPCN 201

Query: 145 NSKNNVKDSSFRRETSE-VTSTDGISQFSAYRE--SSIQDEIGESEEMTYNVAELTEEGQ 204
           NS NN  DSSF RETS+  TS++ IS+ SA+ E  SSIQDEIGESEEM YNVAELT EGQ
Sbjct: 202 NSDNNTGDSSFGRETSKAATSSNEISRVSAFPETPSSIQDEIGESEEMKYNVAELTVEGQ 261

Query: 205 RIIIARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSN---DNESNRRAGSHGSEAVL 264
           RIIIARGGEGGLGN+H LKVSKK KA   +GQEDESI S+    NESN RAG  GSEA+L
Sbjct: 262 RIIIARGGEGGLGNVHALKVSKKHKA---LGQEDESISSDVSETNESNPRAGFPGSEALL 321

Query: 265 VLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVAD 324
           VLELKSIADVGFVGMPNAGKSTLLGAISRAKP+VGHYAFTTLRPNLGNL+YDDLSITVAD
Sbjct: 322 VLELKSIADVGFVGMPNAGKSTLLGAISRAKPIVGHYAFTTLRPNLGNLNYDDLSITVAD 381

Query: 325 IPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERH 384
           IPGLIKGAHENRGLGH+FLRHIERTRVLAYV+DLAA LDG KG PPWEQL+DLVSELE H
Sbjct: 382 IPGLIKGAHENRGLGHAFLRHIERTRVLAYVLDLAAALDGNKGIPPWEQLQDLVSELEHH 441

Query: 385 QNGLSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLVN 444
           Q GLSDRPSL+VANK+DEEGAE++YEELK RV GV IFPVCAVL EGV +LKAGLKSLVN
Sbjct: 442 QKGLSDRPSLVVANKIDEEGAEQVYEELKSRVHGVPIFPVCAVLEEGVAELKAGLKSLVN 501

Query: 445 GDTSYKLKVDDIIV 453
           G+  Y+LKVDDIIV
Sbjct: 502 GEAFYRLKVDDIIV 512

BLAST of Lsi04G000360 vs. TAIR 10
Match: AT1G07615.1 (GTP-binding protein Obg/CgtA )

HSP 1 Score: 479.2 bits (1232), Expect = 3.7e-135
Identity = 253/437 (57.89%), Postives = 319/437 (73.00%), Query Frame = 0

Query: 25  GGNGGRGGDVILECSTALWDFSSLKHHINASRGGHGSSKNKIGTKGADKIVQVPIGTVIH 84
           GGNGGRGGDVILEC+ A+WDFS L+ HI   + GHG+SKN+IG +G DK++ VPIGTVIH
Sbjct: 83  GGNGGRGGDVILECTHAVWDFSGLQPHIKGGKAGHGTSKNRIGNRGEDKVLLVPIGTVIH 142

Query: 85  LVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLLPCN 144
           L EGE+PS V++ S  DLDPW +PG+LV+D                           P +
Sbjct: 143 LQEGEIPSQVENESPKDLDPWDLPGSLVED---------------------------PAS 202

Query: 145 NSKNNVKDSSFRRETSEVTSTDGIS-QFSAYRESSIQDEIGESEEMTYNVAELTEEGQRI 204
              ++V   +      + T  + ++ Q    +E+  +D+  E +++ YNVAELT++GQR+
Sbjct: 203 EENSDVHQETMSESDQDDTEQESLTRQLGMPKEADFEDDDEEIDQIRYNVAELTQQGQRV 262

Query: 205 IIARGGEGGLGNI---HELKVSKKPKASI---PMGQEDESIDSNDNESNRRAGSHGSEAV 264
           IIARGGEGGLGN+     ++ SK  K++I    +   ++  + +D  S+ +AG  GSEAV
Sbjct: 263 IIARGGEGGLGNVSATRYVRGSKFAKSTIRQTNLRSMEDDAEEDDERSSIKAGLLGSEAV 322

Query: 265 LVLELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVA 324
           L+LELKSIADVG VGMPNAGKSTLLGA+SRAKP VGHYAFTTLRPNLGN++YDD S+TVA
Sbjct: 323 LILELKSIADVGLVGMPNAGKSTLLGALSRAKPRVGHYAFTTLRPNLGNVNYDDFSMTVA 382

Query: 325 DIPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELER 384
           DIPGLIKGAH+NRGLGH+FLRHIERT+VLAYVVDLA+ LDG +G  PW+QLRDLV ELE 
Sbjct: 383 DIPGLIKGAHQNRGLGHNFLRHIERTKVLAYVVDLASGLDGCEGLTPWQQLRDLVMELEF 442

Query: 385 HQNGLSDRPSLIVANKMDEEGAEEMYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKSLV 444
           H+ GLSDR SLIVANK+DEEGAEE  +ELK RV+GV IFPVCAVL EGV +LK GLK LV
Sbjct: 443 HEEGLSDRSSLIVANKIDEEGAEERLKELKRRVKGVKIFPVCAVLEEGVAELKDGLKMLV 492

Query: 445 --NGDTSYKLKVDDIIV 453
             NG+ S +LK+++I V
Sbjct: 503 DGNGEPSERLKLENICV 492

BLAST of Lsi04G000360 vs. TAIR 10
Match: AT5G18570.1 (GTP1/OBG family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-38
Identity = 132/422 (31.28%), Postives = 183/422 (43.36%), Query Frame = 0

Query: 24  SGGNGGRGGDVILECSTALWDFSSLKH--HINASRGGHGSSKNKIGTKGADKIVQVPIGT 83
           SGG+GGRGG+V +E   ++      +   H  A RG HG  K + G KG + +V+V  GT
Sbjct: 242 SGGDGGRGGNVYVEVDGSMNSLLPFRKSVHFRAGRGEHGRGKMQSGAKGDNVVVKVAPGT 301

Query: 84  VIHLVEGEVPSVVKHHSSTDLDPWQIPGTLVDDLSSSHQKSLKYSNSEKEVESTFMTTLL 143
           V+                                                          
Sbjct: 302 VV---------------------------------------------------------- 361

Query: 144 PCNNSKNNVKDSSFRRETSEVTSTDGISQFSAYRESSIQDEIGESEEMTYNVAELTEEGQ 203
                          R+  EV              S ++ E GE +E+   + EL   GQ
Sbjct: 362 ---------------RQAREV-------------GSEVEGEEGEEKEV---LLELLHPGQ 421

Query: 204 RIIIARGGEGGLGNIHELKVSKKPKASIPMGQEDESIDSNDNESNRRA--GSHGSEAVLV 263
           R ++  GG GG GN                     S  S  N+  R A  G  G E  L 
Sbjct: 422 RALLLPGGRGGRGN--------------------ASFKSGMNKVPRIAENGEEGPEMWLD 481

Query: 264 LELKSIADVGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYD-DLSITVAD 323
           LELK +ADVG VG PNAGKSTLL  IS A+P + +Y FTTL PNLG + +D D ++ VAD
Sbjct: 482 LELKLVADVGIVGAPNAGKSTLLSVISAAQPTIANYPFTTLLPNLGVVSFDYDSTMVVAD 541

Query: 324 IPGLIKGAHENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERH 383
           +PGL++GAH   GLGH FLRH ER   L +VVD +A         P  +   +  ELE  
Sbjct: 542 LPGLLEGAHRGFGLGHEFLRHTERCSALVHVVDGSA-------PQPELEFEAVRLELELF 546

Query: 384 QNGLSDRPSLIVANKMDEEGAEE---MYEELKCRVQGVLIFPVCAVLGEGVEDLKAGLKS 438
              ++++P ++  NKMD   A E   M++E   R +G+  F + AV  EG  ++ + +  
Sbjct: 602 SPEIAEKPYVVAYNKMDLPDAYEKWPMFQE-TLRARGIEPFCMSAVQREGTHEVISSVYE 546

BLAST of Lsi04G000360 vs. TAIR 10
Match: AT1G72660.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.4e-11
Identity = 42/131 (32.06%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 268 VGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPGLIKGAH 327
           V  +G P+ GKSTLL  ++        Y FTTL    G +HY+D  I + D+PG+I+GA 
Sbjct: 65  VALIGFPSVGKSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGAS 124

Query: 328 ENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPS 387
           E +G G   +   + + ++  V+D A+  +G +        + L  ELE     L+ RP 
Sbjct: 125 EGKGRGRQVIAVAKSSDLVLMVLD-ASKSEGHR--------QILTKELEAVGLRLNKRPP 184

Query: 388 LIVANKMDEEG 399
            I   K    G
Sbjct: 185 QIYFKKKKTGG 186

BLAST of Lsi04G000360 vs. TAIR 10
Match: AT1G72660.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.4e-11
Identity = 42/131 (32.06%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 268 VGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPGLIKGAH 327
           V  +G P+ GKSTLL  ++        Y FTTL    G +HY+D  I + D+PG+I+GA 
Sbjct: 65  VALIGFPSVGKSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGAS 124

Query: 328 ENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPS 387
           E +G G   +   + + ++  V+D A+  +G +        + L  ELE     L+ RP 
Sbjct: 125 EGKGRGRQVIAVAKSSDLVLMVLD-ASKSEGHR--------QILTKELEAVGLRLNKRPP 184

Query: 388 LIVANKMDEEG 399
            I   K    G
Sbjct: 185 QIYFKKKKTGG 186

BLAST of Lsi04G000360 vs. TAIR 10
Match: AT1G72660.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.4e-11
Identity = 42/131 (32.06%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 268 VGFVGMPNAGKSTLLGAISRAKPVVGHYAFTTLRPNLGNLHYDDLSITVADIPGLIKGAH 327
           V  +G P+ GKSTLL  ++        Y FTTL    G +HY+D  I + D+PG+I+GA 
Sbjct: 65  VALIGFPSVGKSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGAS 124

Query: 328 ENRGLGHSFLRHIERTRVLAYVVDLAATLDGRKGKPPWEQLRDLVSELERHQNGLSDRPS 387
           E +G G   +   + + ++  V+D A+  +G +        + L  ELE     L+ RP 
Sbjct: 125 EGKGRGRQVIAVAKSSDLVLMVLD-ASKSEGHR--------QILTKELEAVGLRLNKRPP 184

Query: 388 LIVANKMDEEG 399
            I   K    G
Sbjct: 185 QIYFKKKKTGG 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HSD45.2e-13457.89Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
Q2QZ374.2e-12353.70Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica... [more]
B2UQ306.8e-4942.59GTPase Obg OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 338... [more]
B8FUR92.0e-4834.87GTPase Obg OS=Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) OX=272564 ... [more]
Q24SP02.6e-4834.87GTPase Obg OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=obg PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A5D3CBS71.4e-20686.11Putative GTP-binding protein OBGM OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BG881.4e-20686.11probable GTP-binding protein OBGM, mitochondrial isoform X1 OS=Cucumis melo OX=3... [more]
A0A0A0KWW42.4e-20685.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1[more]
A0A1S3BGW63.5e-20586.11probable GTP-binding protein OBGM, mitochondrial isoform X2 OS=Cucumis melo OX=3... [more]
E5GCK22.1e-19482.79Mitochondrial GTPase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008446933.12.9e-20686.11PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X1 [Cucumis ... [more]
XP_004142409.14.9e-20685.88probable GTP-binding protein OBGM, mitochondrial [Cucumis sativus] >KGN52306.1 h... [more]
XP_008446934.17.1e-20586.11PREDICTED: probable GTP-binding protein OBGM, mitochondrial isoform X2 [Cucumis ... [more]
ADN34199.14.3e-19482.79mitochondrial GTPase [Cucumis melo subsp. melo][more]
XP_022151583.12.4e-19282.03probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_02215... [more]
Match NameE-valueIdentityDescription
AT1G07615.13.7e-13557.89GTP-binding protein Obg/CgtA [more]
AT5G18570.11.9e-3831.28GTP1/OBG family protein [more]
AT1G72660.14.4e-1132.06P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G72660.24.4e-1132.06P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G72660.34.4e-1132.06P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 289..307
score: 40.29
coord: 316..331
score: 53.23
coord: 268..288
score: 54.27
coord: 333..351
score: 37.3
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 267..393
e-value: 5.0E-24
score: 84.6
IPR014100GTP-binding protein Obg/CgtAPIRSFPIRSF002401GTP-binding_obgcoord: 21..449
e-value: 3.2E-86
score: 287.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 265..448
e-value: 1.5E-49
score: 170.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 258..437
IPR006169GTP1/OBG domainPFAMPF01018GTP1_OBGcoord: 24..86
e-value: 1.0E-16
score: 61.0
IPR006169GTP1/OBG domainPROSITEPS51883OBGcoord: 1..265
score: 32.002544
IPR036726GTP1/OBG domain superfamilyGENE3D2.70.210.12GTP1/OBG domaincoord: 17..264
e-value: 9.3E-31
score: 108.5
IPR036726GTP1/OBG domain superfamilySUPERFAMILY82051Obg GTP-binding protein N-terminal domaincoord: 24..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 223..252
NoneNo IPR availablePANTHERPTHR11702:SF42BNAA08G29050D PROTEINcoord: 166..452
NoneNo IPR availablePANTHERPTHR11702:SF42BNAA08G29050D PROTEINcoord: 24..118
NoneNo IPR availablePROSITEPS51417ARFcoord: 259..440
score: 8.797297
IPR045086OBG-type GTPasePANTHERPTHR11702DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATEDcoord: 166..452
IPR045086OBG-type GTPasePANTHERPTHR11702DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATEDcoord: 24..118
IPR031167OBG-type guanine nucleotide-binding (G) domainPROSITEPS51710G_OBGcoord: 266..438
score: 66.493141
IPR031167OBG-type guanine nucleotide-binding (G) domainCDDcd01898Obgcoord: 266..437
e-value: 7.7761E-77
score: 235.397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000360.1Lsi04G000360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042254 ribosome biogenesis
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0000287 magnesium ion binding