Lsi04G000120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G000120
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
Locationchr04: 191152 .. 191430 (+)
RNA-Seq ExpressionLsi04G000120
SyntenyLsi04G000120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTCACAGTTCGAGGTCTGAAGGTTCCGCCGAACTCCGCCAGCCTTGAAGAAGCCCGGAATCGAGTCTTCGACTTCTTCAGATCAGCATGTAGATCATTGCCAGCAATCATGGACATTTACAACTTAGATGATGTCGCTACTGTTTCTCAGCTCCGTTCTACGATCGCCTCCGAGATCCGCAAGAATTCCCACGTCAGCGACCCTAAGGTCTTCTCGTTCCTGCAATTTACTCTATTTGCGTCTAAATTCTATATGATTTTGGGGGCGGCTTAA

mRNA sequence

ATGGCATTCACAGTTCGAGGTCTGAAGGTTCCGCCGAACTCCGCCAGCCTTGAAGAAGCCCGGAATCGAGTCTTCGACTTCTTCAGATCAGCATGTAGATCATTGCCAGCAATCATGGACATTTACAACTTAGATGATGTCGCTACTGTTTCTCAGCTCCGTTCTACGATCGCCTCCGAGATCCGCAAGAATTCCCACGTCAGCGACCCTAAGGTCTTCTCGTTCCTGCAATTTACTCTATTTGCGTCTAAATTCTATATGATTTTGGGGGCGGCTTAA

Coding sequence (CDS)

ATGGCATTCACAGTTCGAGGTCTGAAGGTTCCGCCGAACTCCGCCAGCCTTGAAGAAGCCCGGAATCGAGTCTTCGACTTCTTCAGATCAGCATGTAGATCATTGCCAGCAATCATGGACATTTACAACTTAGATGATGTCGCTACTGTTTCTCAGCTCCGTTCTACGATCGCCTCCGAGATCCGCAAGAATTCCCACGTCAGCGACCCTAAGGTCTTCTCGTTCCTGCAATTTACTCTATTTGCGTCTAAATTCTATATGATTTTGGGGGCGGCTTAA

Protein sequence

MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASEIRKNSHVSDPKVFSFLQFTLFASKFYMILGAA
Homology
BLAST of Lsi04G000120 vs. ExPASy Swiss-Prot
Match: Q9LHI0 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Arabidopsis thaliana OX=3702 GN=At3g12260 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.2e-21
Identity = 48/76 (63.16%), Postives = 59/76 (77.63%), Query Frame = 0

Query: 3  FTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASEIR 62
          F +R + VPPNSA+L EAR RVFDFFR+ACRS+P IMDIYNL DV   SQLR  I+++IR
Sbjct: 5  FALRKIGVPPNSANLTEARRRVFDFFRAACRSIPTIMDIYNLQDVVAPSQLRYAISAQIR 64

Query: 63 KNSHVSDPKVFSFLQF 79
           N+H++DPKV   L F
Sbjct: 65 NNAHITDPKVIDLLIF 80

BLAST of Lsi04G000120 vs. ExPASy TrEMBL
Match: A0A5A7SZJ9 (Peptidyl-prolyl cis-trans isomerase CYP22 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007970 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.9e-36
Identity = 80/83 (96.39%), Postives = 81/83 (97.59%), Query Frame = 0

Query: 1   MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
           MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV TVSQLRSTIASE
Sbjct: 227 MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTVSQLRSTIASE 286

Query: 61  IRKNSHVSDPKVFSFLQFTLFAS 84
           IRKNSHV+DPKVF FLQFTLFAS
Sbjct: 287 IRKNSHVTDPKVFPFLQFTLFAS 309

BLAST of Lsi04G000120 vs. ExPASy TrEMBL
Match: A0A1S3BH79 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucumis melo OX=3656 GN=LOC103489526 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.0e-31
Identity = 72/78 (92.31%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV TVSQLRSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTVSQLRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. ExPASy TrEMBL
Match: A0A0A0KRV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G624440 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-30
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEAR+RVFDFFRSACRSLPAIMDIYNLDDVATVSQLRS IASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARSRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSAIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. ExPASy TrEMBL
Match: A0A6J1H232 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucurbita moschata OX=3662 GN=LOC111458871 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.7e-30
Identity = 69/78 (88.46%), Postives = 72/78 (92.31%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIM+IYNLDDV T SQ+RSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMNIYNLDDVTTTSQIRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. ExPASy TrEMBL
Match: A0A6J1JDJ2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucurbita maxima OX=3661 GN=LOC111483420 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.6e-30
Identity = 68/78 (87.18%), Postives = 72/78 (92.31%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIM+IYNLDDV T+S +RSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMNIYNLDDVTTISHIRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. NCBI nr
Match: KAA0034771.1 (peptidyl-prolyl cis-trans isomerase CYP22 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 159.5 bits (402), Expect = 1.4e-35
Identity = 80/83 (96.39%), Postives = 81/83 (97.59%), Query Frame = 0

Query: 1   MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
           MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV TVSQLRSTIASE
Sbjct: 227 MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTVSQLRSTIASE 286

Query: 61  IRKNSHVSDPKVFSFLQFTLFAS 84
           IRKNSHV+DPKVF FLQFTLFAS
Sbjct: 287 IRKNSHVTDPKVFPFLQFTLFAS 309

BLAST of Lsi04G000120 vs. NCBI nr
Match: XP_038892169.1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Benincasa hispida])

HSP 1 Score: 144.1 bits (362), Expect = 6.2e-31
Identity = 72/78 (92.31%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV TVSQLRSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTVSQLRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. NCBI nr
Match: XP_008446979.1 (PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Cucumis melo])

HSP 1 Score: 144.1 bits (362), Expect = 6.2e-31
Identity = 72/78 (92.31%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV TVSQLRSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTVSQLRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. NCBI nr
Match: XP_023552714.1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 1.4e-30
Identity = 70/78 (89.74%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDV T+SQ+RSTIASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVTTISQIRSTIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. NCBI nr
Match: KAE8648872.1 (hypothetical protein Csa_008170 [Cucumis sativus])

HSP 1 Score: 141.7 bits (356), Expect = 3.1e-30
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MAFTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASE 60
          MAFTVRGLKVPPNSASLEEAR+RVFDFFRSACRSLPAIMDIYNLDDVATVSQLRS IASE
Sbjct: 1  MAFTVRGLKVPPNSASLEEARSRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSAIASE 60

Query: 61 IRKNSHVSDPKVFSFLQF 79
          IRKNSHV+DPKV   L F
Sbjct: 61 IRKNSHVTDPKVIDMLLF 78

BLAST of Lsi04G000120 vs. TAIR 10
Match: AT3G12260.1 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 103.6 bits (257), Expect = 8.6e-23
Identity = 48/76 (63.16%), Postives = 59/76 (77.63%), Query Frame = 0

Query: 3  FTVRGLKVPPNSASLEEARNRVFDFFRSACRSLPAIMDIYNLDDVATVSQLRSTIASEIR 62
          F +R + VPPNSA+L EAR RVFDFFR+ACRS+P IMDIYNL DV   SQLR  I+++IR
Sbjct: 5  FALRKIGVPPNSANLTEARRRVFDFFRAACRSIPTIMDIYNLQDVVAPSQLRYAISAQIR 64

Query: 63 KNSHVSDPKVFSFLQF 79
           N+H++DPKV   L F
Sbjct: 65 NNAHITDPKVIDLLIF 80

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHI01.2e-2163.16NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Arabidopsis thal... [more]
Match NameE-valueIdentityDescription
A0A5A7SZJ96.9e-3696.39Peptidyl-prolyl cis-trans isomerase CYP22 isoform X1 OS=Cucumis melo var. makuwa... [more]
A0A1S3BH793.0e-3192.31NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucumis melo OX=... [more]
A0A0A0KRV01.5e-3091.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G624440 PE=3 SV=1[more]
A0A6J1H2325.7e-3088.46NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucurbita moscha... [more]
A0A6J1JDJ29.6e-3087.18NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
KAA0034771.11.4e-3596.39peptidyl-prolyl cis-trans isomerase CYP22 isoform X1 [Cucumis melo var. makuwa][more]
XP_038892169.16.2e-3192.31NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Benincasa hispida][more]
XP_008446979.16.2e-3192.31PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Cucumis... [more]
XP_023552714.11.4e-3089.74NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 [Cucurbita pepo sub... [more]
KAE8648872.13.1e-3091.03hypothetical protein Csa_008170 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G12260.18.6e-2363.16LYR family of Fe/S cluster biogenesis protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016488NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6PANTHERPTHR12964NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNITcoord: 1..78
NoneNo IPR availablePANTHERPTHR12964:SF4NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6-LIKEcoord: 1..78
NoneNo IPR availableCDDcd20266Complex1_LYR_NDUFA6_LYRM6coord: 17..72
e-value: 1.04148E-23
score: 83.7663

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000120.1Lsi04G000120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0000413 protein peptidyl-prolyl isomerization
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0005747 mitochondrial respiratory chain complex I
molecular_function GO:0003755 peptidyl-prolyl cis-trans isomerase activity