Lsi03G022080 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G022080
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr03: 33094073 .. 33096293 (-)
RNA-Seq Expression	Lsi03G022080
Synteny	Lsi03G022080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGAAAGCCAAGTGAGTCCCACTTCCCTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCCGAATTTGCTTCGAAAGCCCTTTGGCGAAGAACTACACACAGAAAGGGGATTTCATAATTTCATATCTTGGATTTCTTTTAGAGGTGCGCCGTTGAAGACGAACGAGGTGTAAGGAATTTTCGCTTGCAGATTGCATAGGTATTTATTTTTGTTGTTTTTGAGTTTCGTTTTTTGTGTTCTTTATGGTTTATTCTGAATTGAATGGAAATTTATCTATTGAATTGAATTAGATGAGGTAGGAGTTGTTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTCTAAGTGTGGAAATTAAGTCAAAATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTGTAATCTCTTCATATGCGGTTCAACTTTCTTATAATTTGATATTTACTCTGGATTCTGTGAGAATTGCTAATGCAATGTTTGTTTCGTTGTCTGGAAATTATCAGCTATGTTTGTTAGATTTCTATGATCGAAACTGAATCTAGTTTCAATGTTTTGTATCTTATGATCTATGTTTTCTCTATGCTTAGATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTTCAGGAAAGTTTCTCTGAAAGTAGTTCGATAGGTTATGCTGTCTGAGAGAGCTGCAAGTTTTTAGGTTTTGCATCTCATTACCTGTACATTCTTCCTTTCTTATTAGATTAGATATTAGTCGCTTCGATCTTATTCGTGGAATTGTTCTGCGCCCACTCAAATACGTTGTTTCCTGCTCTAAGATTTATGGAGCTATTTATTTAAGATTTAATAATAACTTTTGTGTTTCTTATTTTATAAATGTTGAGTAATTTGGATATGTATTTTCTTCCTTTTGTGTGGGTTATGTTGTATGCTTAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGTATGACAATTATAGCCTTGAACTGCAACATATATTTTCTTTCAAATTGGAGATTTTTGTTGTTTTTAGTGTGCAATAGTTCCGGTTGAGGCTTATTTAATTCATAAGCATTGAAATAAAACACTTCAAATCTGAACAGGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGTACTGCAGCCTGCATGAGAAATGACATCTTTGACTCCTACATTGTCCAAGTGTGGTGATTGAAGCCGATCACTAAAGATAAAAATCTGATGAAGTAGCTTATACTACTTGCCTCTACACATATCTTGTAAATCCTGTGAATGTGATGAATTTGTTTCAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGTAAGTGGCTAAGAAGATAAAAGCTGCCCGTTCTTCAACTTAAGGTGCATTAAAATTTGGAGTCTTGCTAATCCTATATTTTAGTTCTGCAAATACAGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGTAAGCTTTCTGCTGTTTTCATCTGCTATCAGTTTCTAATTGAGTACTAGTCCTTTCTTTGCAAAAAAGGGTTGGATTATTTGTGGGTTATTTGAAGCTGATTTTTGCAACTTGTTATCTTTGTTTCAGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

mRNA sequence

GCGAAAGCCAAGTGAGTCCCACTTCCCTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCCGAATTTGCTTCGAAAGCCCTTTGGCGAAGAACTACACACAGAAAGGGGATTTCATAATTTCATATCTTGGATTTCTTTTAGAGGTGCGCCGTTGAAGACGAACGAGGTGTAAGGAATTTTCGCTTGCAGATTGCATAGATGAGGTAGGAGTTGTTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTCTAAGTGTGGAAATTAAGTCAAAATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTAATTGCTAATGCAATGTTTATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

Coding sequence (CDS)

ATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTAATTGCTAATGCAATGTTTATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

Protein sequence

MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY

Homology

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BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 6.1e-96
Identity = 175/280 (62.50%), Postives = 205/280 (73.21%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DG
Sbjct: 61  ----LASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++KDR DKGK+SE
Sbjct: 121 KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGIC+E+ +KVVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWI 237

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISY 281
            T S+EI DL  I +ENLKRL ++I+KLP +  DP L+ Y
Sbjct: 241 YTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot
Match: Q803C1 (E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-05
Identity = 34/105 (32.38%), Postives = 52/105 (49.52%), Query Frame = 0


Query: 127 QERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK-FKRKDRLDKGKI----SEI 186
           ++ + +LKE   VI   L    +G  ++ + K +E+   K  K K +  K ++    +E+
Sbjct: 327 KQLEEALKEHRKVI-EELKHAWQGFKEVLQAKDKELEVTKEEKEKAKAQKEEVVTQMTEV 386

Query: 187 DLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFC 227
            LE E +C IC EL  + V  +C HS C  C   WR R   CP C
Sbjct: 387 -LESELQCSICSELFIEAVTLNCAHSFCQHCISEWRNRKDKCPMC 429

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BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot
Match: Q9UHC7 (E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 181 IDLEREEECGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCP 240
           +   ++  CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP
Sbjct: 273 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 332

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BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot
Match: Q9TT91 (E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 181 IDLEREEECGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCP 240
           +   ++  CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP
Sbjct: 269 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 328

[Close]

BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot
Match: Q9QXP6 (E3 ubiquitin-protein ligase makorin-1 OS=Mus musculus OX=10090 GN=Mkrn1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/68 (36.76%), Postives = 33/68 (48.53%), Query Frame = 0


Query: 189 CGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCPFCRDSLKR 241
           CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP CR +   
Sbjct: 281 CGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNF 340

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BLAST of Lsi03G022080 vs. ExPASy TrEMBL
Match: A0A0A0LA78 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 234/285 (82.11%), Postives = 239/285 (83.86%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKR DRL+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISR+NLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. ExPASy TrEMBL
Match: A0A5D3DMZ7 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00850 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.1e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. ExPASy TrEMBL
Match: A0A1S3B5N9 (uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.1e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. ExPASy TrEMBL
Match: A0A6J1CVC0 (uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.4e-119
Identity = 226/285 (79.30%), Postives = 234/285 (82.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KRKD+LD+GKI+E
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN  VVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            T SSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 YTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. ExPASy TrEMBL
Match: A0A6J1F2V6 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441644 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.8e-117
Identity = 222/285 (77.89%), Postives = 230/285 (80.70%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KRKD+LDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLP+C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSS+EIVDL FIS ENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSTEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. NCBI nr
Match: XP_038904042.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida])

HSP 1 Score: 456.1 bits (1172), Expect = 2.3e-124
Identity = 236/285 (82.81%), Postives = 237/285 (83.16%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCH AGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHFAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWR RSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRARSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. NCBI nr
Match: XP_011651863.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN58763.1 hypothetical protein Csa_000946 [Cucumis sativus])

HSP 1 Score: 455.3 bits (1170), Expect = 3.9e-124
Identity = 234/285 (82.11%), Postives = 239/285 (83.86%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKR DRL+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISR+NLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. NCBI nr
Match: XP_008442427.1 (PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK24968.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 451.8 bits (1161), Expect = 4.3e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. NCBI nr
Match: XP_022145744.1 (uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145745.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145746.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia])

HSP 1 Score: 439.1 bits (1128), Expect = 2.9e-119
Identity = 226/285 (79.30%), Postives = 234/285 (82.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KRKD+LD+GKI+E
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN  VVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            T SSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 YTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. NCBI nr
Match: XP_023527445.1 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 3.5e-117
Identity = 223/285 (78.25%), Postives = 230/285 (80.70%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KRKD+LDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLP+C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDL FIS ENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 4.3e-97
Identity = 175/280 (62.50%), Postives = 205/280 (73.21%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DG
Sbjct: 61  ----LASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++KDR DKGK+SE
Sbjct: 121 KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGIC+E+ +KVVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWI 237

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISY 281
            T S+EI DL  I +ENLKRL ++I+KLP +  DP L+ Y
Sbjct: 241 YTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of Lsi03G022080 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 4.8e-88
Identity = 162/282 (57.45%), Postives = 196/282 (69.50%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            +A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -VALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++ KD  +K K+SEID+
Sbjct: 125 MSVYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+N+ VVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+   
Sbjct: 185 EREEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLME 240

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            S+ V++  I REN KRLF++I KLP +VPD    S   +PY
Sbjct: 245 KSDTVNMYTIERENKKRLFVYIEKLPLVVPDQVFAS---SPY 240

[Close]

BLAST of Lsi03G022080 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.9e-85
Identity = 163/282 (57.80%), Postives = 196/282 (69.50%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            LA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERKAS++EF  VI PSL QLQRG+TDI++ KQ+EV   ++++K   D+ ++SEI++
Sbjct: 125 MSVYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMRYRKK---DESEMSEIEI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+NSKVVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+   
Sbjct: 185 EREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLD 237

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            ++ V+L+ I+REN KRLFM+I KLP +VPD     Y  +PY
Sbjct: 245 QNDTVNLTAIARENQKRLFMYIEKLPLVVPDQ---VYASSPY 237

[Close]

BLAST of Lsi03G022080 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.5e-85
Identity = 145/220 (65.91%), Postives = 178/220 (80.91%), Query Frame = 0


Query: 66  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 125
           A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTMS
Sbjct: 36  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 95

Query: 126 IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLER 185
           + ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++ KD  +K K+SEID+ER
Sbjct: 96  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 155

Query: 186 EEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 245
           EEECGICME+N+ VVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    S
Sbjct: 156 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 215

Query: 246 EIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           + V++  I REN KRLF++I KLP +VPD    S   +PY
Sbjct: 216 DTVNMYTIERENKKRLFVYIEKLPLVVPDQVFAS---SPY 252

HSP 2 Score: 41.2 bits (95), Expect = 1.6e-03
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 4  SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFE---VVCELSHCPASSFVRYL 61
          SFKDSLKALEADIQ ANT+++N +   +    +    E +   V   LS+ PA+ F+ +L
Sbjct: 5  SFKDSLKALEADIQHANTVLSNELGDEVIFRALDYPREKDGARVQMRLSYTPAAQFLLFL 64

[Close]

BLAST of Lsi03G022080 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.2e-76
Identity = 152/282 (53.90%), Postives = 184/282 (65.25%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            LA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERKAS++EF                DI++ KQ+EV   ++++K   D+ ++SEI++
Sbjct: 125 MSVYERKASIREF---------------QDIDDSKQKEVCKMRYRKK---DESEMSEIEI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+NSKVVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+   
Sbjct: 185 EREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLD 222

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            ++ V+L+ I+REN KRLFM+I KLP +VPD     Y  +PY
Sbjct: 245 QNDTVNLTAIARENQKRLFMYIEKLPLVVPDQ---VYASSPY 222

The following BLAST results are available for this feature:

BLAST of Lsi03G022080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	6.1e-96	62.50	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]
Q803C1	1.3e-05	32.38	E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1	[more]
Q9UHC7	1.9e-04	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3	[more]
Q9TT91	1.9e-04	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 ...	[more]
Q9QXP6	1.9e-04	36.76	E3 ubiquitin-protein ligase makorin-1 OS=Mus musculus OX=10090 GN=Mkrn1 PE=1 SV=...	[more]

BLAST of Lsi03G022080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA78	1.9e-124	82.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 P...	[more]
A0A5D3DMZ7	2.1e-123	81.40	E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B5N9	2.1e-123	81.40	uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=...	[more]
A0A6J1CVC0	1.4e-119	79.30	uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1F2V6	3.8e-117	77.89	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cuc...	[more]

BLAST of Lsi03G022080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904042.1	2.3e-124	82.81	E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida]	[more]
XP_011651863.1	3.9e-124	82.11	E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin...	[more]
XP_008442427.1	4.3e-123	81.40	PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ...	[more]
XP_022145744.1	2.9e-119	79.30	uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145...	[more]
XP_023527445.1	3.5e-117	78.25	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucur...	[more]

BLAST of Lsi03G022080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01520.1	4.3e-97	62.50	RING/U-box superfamily protein	[more]
AT3G47160.1	4.8e-88	57.45	RING/U-box superfamily protein	[more]
AT5G58787.1	2.9e-85	57.80	RING/U-box superfamily protein	[more]
AT3G47160.2	6.5e-85	65.91	RING/U-box superfamily protein	[more]
AT5G58787.2	7.2e-76	53.90	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 189..226 e-value: 2.2E-6 score: 37.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 189..227 score: 12.194218
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 186..232 e-value: 1.3E-9 score: 37.7
None	No IPR available	PANTHER	PTHR15315:SF31	E3 UBIQUITIN-PROTEIN LIGASE AIRP2	coord: 1..25 coord: 64..281
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 1..25 coord: 64..281
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..232
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 164..235 e-value: 6.3E-15 score: 56.3
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 204..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G022080.1	Lsi03G022080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009789	positive regulation of abscisic acid-activated signaling pathway
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity