Lsi03G020940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G020940
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic
Location	chr03: 32198846 .. 32205399 (-)
RNA-Seq Expression	Lsi03G020940
Synteny	Lsi03G020940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACTTTTTTACATACTTCCCCTTCCCTTCCTTCTATAAATTTCCCTCCTCTTTCTTTCTTCTCTGCCTCCCCCCATTTCCACCGCCGTTCCCCTCGCCGCCACCACCGCCACCGCCGTCCAAAAAAAATATTCAAAATTATTTCATTCCACCTTATTCTCGTGAGAAATTTTGGTGAAAAGAGATCAATATAATGTCCATCATTTCTCTAATTACTGGATGGCCGGGGCCCAGCGGCTTCGGCTCGGCCTCCACGGCGGAGGACGTCACTCAAGGGATCGACGCCGCCGGCCTTACCGCCATTGTCACTGGTCATTTCTCTCTCTCCAAATTTGTTTCTTTATTTCATTTATTTATTTTTTTCAGTCAATTTTTTTTTTCTTTTTCTTTTTTTCTTTTGGGGGTATGTTGTTTTGTTTTATTTATAATTTTTTTAGGGAAAAAAAAGTCTTTACTTTTGTTTTTGTTTAACATGTAGTAGTAGTACTATGTTGTTCTATCTCTTTCTGTATGTATTTATTTATTTAGAAAGAATCTAATTAAACTTATTAATCTTTTTAGTCTACTAGAAAGTCAACTTTAATTTTTTTTAGTTCAATTTCAAAAGAAGAAAATTTGACTTTATTGAAACTAATCTATCATTAATCTATTATTGGAATATTTTTGTGAACTTTTAATTTTTTTTGTCTTACACTCAATTTTTATCTCTAATAAATTTATGATAAATTTAAAACTAATCTAATATAAATTTATAATTTTAAAAGTCTGCGAATCAAATAAATTTAAAATTTAGGGACTAAATTAATCGAACACTAAATTTATACATTACCATACATTTAATTTCTTAAAGTTCATTTTAAGTGTTTATTCTTGCGTTTGACTATTAATTTTACTGTTAAGGGGCTTTTATATATTAACATGAAAATGAGAATAATTTGTATTTTTCTTAAGTCTGCTTGATATATATTTTTTAGTGTTTTTTTTTTTAATTCCTAGTCAAAATCCATGAAGAAATATTTTTTTTTTTAATTTTCAAAACTCGTTTTAATTTTGAAAAAATTGTAAAAAAAATAAATAACAAAAGAAAAAAAATCATAAGTGAAATAGTATATAAACTTACTTTAGAATAAATAAATAAAACTAAATAGTTATCCAATTGTCCAACTTTAAATTGAAGACCACTAGTCCAAAAGTCTAACAACTTGTTTTAATTAGCTTAAAAAAATAAATGTTTTTTAAAAATTAATTTTCATTTAAACACTTTTGATAAGAATCGTTTATAATAAAGATACTAATGATTTTCAAAATTCATTTTGGGTAATTATCAAACACTTATATTTCTTTAAAAAATGAGTTATTTTTTTAAATTAAACATTTGAAAAAGTATTTTTAAAACACCCTAAATCAGTTATTTGTATTTCTTTTTCAAATATTTTGTCCAATAGTTTATGAAACTTACACATATCTTTTATTAAGTAACAGTAGAATGTAGATAAAGGTAAATTTATTTTCTCTCTAAATATTTTGTCCATAATATGTTTTATTTTATATTAGTACAATAATTGTGAAGATAAAAGGACTAAATCTCTGATTTCCCATTATCAATTACCATTAAATGAAGTTCAGATCATTATTGAATTTAAAAATTTTACCCAAACAAAAATAATTAGAAAATGACTTTATAATAATTTGAATTAAGAATTTCATATTCAGCTACCTATTGTCACACCAACATTCAATTGGTAAGGTTAGGTTGAAACATTAAGGCAAATCTTTAAAAAAAAAACATTCAATTGTGTGTAGTTGTTTTCAAATATAGAAAAATGAGTCAAAATATTTACAAATATAAAATAATTTCAATGTTTATCGCAGTTTATAGACAGTAAATTTTTTTTTTATATTTATAAATAGTTTGAGATTTTATTTATTTATTGTCCTAGTTTCCAATGATGGAAAAGATTGGAAAAGAGTATTGAATGGGTGGTATTGTATCACAAGATGGGGAAAGGCTCATTTGTCTAAAAGCATTAAAGTTAGAAGCAAGTAGGCATATAGAGAAAATCAAATTGAAAGATATTTATCACATATGACCTGCAACGTAGGGAAATTAGGAGAAAAAAAAAACAAAAAAAAAATTTGATTCCGACCCCAAACTCATCTAGTTGTATTAGTTTCTTAACTATGAACTTTCGATTTCATTCAAAATGTCGTTACAAAACGTACAATGCGGATCAGTTGATTAAATCTTAGTGAACAACTACTTTGTAGCTTTTTTTTTTTGTTCAAATTCATGAATTTCAGCAAAGTTATTCAATTGTTAATTTATACATTGGTTAAACGTGTAAAAGTCCATTTTATTGACAGTATTTTTATATTGTTTTTGTTAAAATTAGTGGAAATTGAATCAAGAGTAAAAATTGTAAAATATAGATTTAAGTTTAGAGTCTAATTTGATGAAATTGTTATATTATAGGAGGAGCGAGCGGAATTGGATTAGAAACAGTGAGAATTTTAGTGATGCGAAAGGTGCACGTGATTATAGGAGCAAGAAATTTAGAGGCAGCAAATAAAGCAAAACAACAACTAGTTGAAGAAAATCCATCTGCTAAATTGGATGTTCTGAAACTTGACCTTTCATCCATCAAATCCACAAGAGAATTTGCTGAAAATTTCATTGCTCTTAATCTCCCTCTCAACATTTTAATGTAAATCTCTCTCTTTTAATTCTTATTTTTCTTTCAAAACTTTTTATTTAGACTAAATTAAAAAATAAAAATAACAAGGATTGTTTTCAAATATAAAAAAATGAGCTAAATTATTTATAAATATAGAAAATTTTTACTGTCTATCAGCGGTAGACTGCGATAGAATTCTATCGCAATCTATCACTAATAGACAGTAAAAAAAATTTATATTTATAAATAGTTTGATATTTTTTCTATTTATAATAATTTTTCAATAAAAAAAATACCCCTCAATGAACTTCACTCCTTTTTCAAAAATTACGATATTATCCATGACTTTTCTTCTCTCTTTTTCTTCAAATTCTTATTTTTTTTTCAAAACTCGTTTATTTAGACTAAATTATATAAACACCCCTCAATGAACCTCACCCCTCCTTCAAAAATACTATTATTTTTCAAAAATTACAATATTACCCTTGACTTTTCAAAAGCACTTCAAAACTATCATTTGAGTAGAAAAATCATTAGAATGTTGGATGAAAAGTCGACATATTTGAATGTTTTCTTTCATGTCACAGTCATATCATTTCATATCACCGCCACTTCGATTTTTCATCGAACATTAACAAATGATTTCTAGTAATTTAAGAGTAGTTTTGAAACGTTTATAGAAAACAAAGAAGAGCATTGCAAGTTTTTTTTTTTTAAAAAAAAAACATAGAACTATAAAGTTTAGAAGTTTTTTTAAGGTTGTTTTCAAATATAAAAAAATGAGTAAAATTTTTTATAAATATAGAAAAAAATTCAATGTCTATCAGGGATAGACTTCTATCATGAATAGACAATAAAATTTTTTTATATTTGAAAACAACCTTAAAAAAACTTCTAAACTTTATAGTTCTATTTTTTTTTTTTAAAAAAAAAACATAGAACTATAAAGTTTAGAAGTTTTTTTTAAGGTTGTTTTCAAATATAGAAAAATGAGTAAAATTATTTATAAATATAGAAAAAAATTCAATGTCTATCAGGGATAGACTTCTATCGTGAATAGACAATAAAATTTTTTTATATTTGTAAATAGTTTGATATTTATAGTAATTTCTCCTTTTTTTTTTATTATTAATAATAATTTAGGCATTTATTTATGAGATCCTTTTGAAGTGACATTTTGGGTGCAATTTTAGAAACAATGCTGGAGTTATGTTTTGCCCATTTCAACTTTCTGAAGATGGAATAGAGATGCAGTTTGCCACCAACCATCTTGGTAATTTACAATATCCAAATTACCCTTCAATTACCATCTTTCATTTCTATTTCCTCTATCATAAAAATAAAAAAATAAAAAAGACATTAGATTTCATCATATACTTTAATTAGTACCTTAACAAGTTTAAAATTTTTAAGAGGAGTTTTAACTCATAATTTTTTTTTGGGTTAAAAAACATGTTTAATCCATGAAATTTCATAAAAGTAACAATTTAGTCTCTAGACTACAGTTGGTAACCATTTAATTCCTATACTTTCACATTTGTAGTAATTTAGTCTATGTACTTTAAGAAGTAACAATTTAGTTTTTGTACTTTACAATTTATTACGATTTAGTCCATATCGTGAAAAAAATTAGTAATGAAATTTCTTCTAAGTGTAGATTGATTAACTAATTGGAAATCAAATTTATTTGTAAACTATAAAGACTAAGTCGTTGCGCAATAAAAGTTGGCACGTAATTTTGATGAAATTTATCATAATAGGGACTAAATCGTTACTTATTAAGGTTCAATGACTAAATAATTTTTTTTCATGAAAGTTCAAGAATCAAAGGTGTTTTTTAACCTAATTTTTTAGTTCAACATAAGGTGGAAGTTTTGTCGAAATTGATAGTACATATTTTATCAGTTGAGTTAAGCTCACTTTTACAGCTTTAAATTATAATTAACAAAAAGAAAAATGTGCAATAGATAGATGAAAGGATATAAAAGTCGTATAATTATTTTGTGTGTAATTATACTGTTAATTTTTTTTTAGGTCATTTTCTGTTAACAAATCTTCTTCTTGAGAAAATGAAGAACACTGCAAAAAGCACAGGAATTGAGGGTAGAATTGTGAATTTGTCATCTATAGCTCATGTCCACACTTATGGAGATGGAATTCGCTTCAATAAACTCAATGATAAGAATGGGTATTTTATTATTATTATTATTATTATTATTATTATTTATTTATTTATTTATTTATGGAGTTTTGAATTAATGCTTGTTTTTTATTTTTTATTTTTTATTTTTAACTTTTGGTGTAATTATAAAAGCTAAGTTATTAAATTGTAGGTATTCTGATAAAAGAGCATATGGGCAATCAAAATTAGCCAACATTTTACATGTCAAGGAGCTCACTCGTCGATTCCAAGTAATTCTCTAAACCTTTTAGATTTTAAAATTTTACTACTTTTTCTTTATTACATTCTAAAATGTGACAATTATTATCTTCCTTTTATTATTTTTCTTAAATTGTTGAAGCAATTTTAAATTTTGGGTTAAATTATAAGATTAGTCCTTCAACTTTTAGATTAACGTAAAATAAATTTATAAACTTATAAAAGTTAGGATGAGTTTCTATACTTTCAACTTGTTTGACTAATAGGTCTAGTCCCTCAACTTAAAAAAAAATTATATACATATATATATTCTTAAATCTTCAATTTTGCATCTAATAAATTTTTAATTTATTCAATATTAAAAAGAAAAATTATAAACAAATTGAATACTAATTACTTGAGTGGTAGATCTACCACTCAAGTGATAGAATTCTATTGTTGTTTATACTGATAGACAGTAAATAATTTATATATTTGTAAATAATTTTGCTCATTCTTTTATATTTAAAAATGTGCCAAAAAATTATTTATTTATTTGCGGTAAATAAACAATTCATTTTTTACTATTGAATTAGATATTAAAGAAATTTTGTTGGTTGTAGGAAGAGGGTGTGAACATAACGGCAAACGCGGTGCATCCAGGATTGATAATGACTCCTCTTATGAGGCATTCAATGCTTCTTATGCGTACGACATATCGACACTTTTTTTTTTCTTTTTTATACTAGTTTCGTCTAGCCGACATGTCGTCACTTACCCACCTTAATTTCTCTCTCTTCCAGGGCTTTTGCAGGCTTTCACCTTCTTTATCTGGAAGAATGTCCCTCAGGTAAATTCAAGCAATTGCATGCCTTTTCATATTCTTATTTTCTAATGAAATTTTCTCAGTGCAACTTTTTTAAACTTATTTTTAATGTATTTATTTCAGAAATAGCTAATGTAGTTTAAAGTGTATTTATTAAAGAGATTGTTTGTTTCTTAGATAAATGGTTTTAGTCTTTTAGATATAAATGTTTAAAATATATATATGTTGTTTTTGTTTTAGGGGGCATCGACAACATGCTACGTTGCGCTCCACCCGAGTTTGAAGGGAGTGAGTGGGAAATATTTTTTGGACAACAACGAGAAGCGACCGAGCTCCTATGCTAGAAATGGAAAACTTGCAAAGAAACTTTGGGACTTCAGCAAGGACTTGATAAGTTCAGTGTCTAAAGCATGAAAAAGAAGTGGCATTTAAACCTACGTTACCCCACAAAAACCTCAAGTCACATGCTAGCTCAACTTTATAAAGAATTAAGAGAGATTCAATAATGTGGTTCTTAAGAATGAAAAAATATTATAAATAGTTTTCTTATTAAAAGAGAGAGAGAGAGAGAGAGAAGATGAAGTGGTTTTGTGGACATTGGAAGCCCCTAGTGTTTGTGTGATTATTCCATTCATATTGTTTGATAATTATGATATTCAATCGATTACCTAAGTATATATGGTTTCTTTTTCCAA

mRNA sequence

CACTTTTTTACATACTTCCCCTTCCCTTCCTTCTATAAATTTCCCTCCTCTTTCTTTCTTCTCTGCCTCCCCCCATTTCCACCGCCGTTCCCCTCGCCGCCACCACCGCCACCGCCGTCCAAAAAAAATATTCAAAATTATTTCATTCCACCTTATTCTCGTGAGAAATTTTGGTGAAAAGAGATCAATATAATGTCCATCATTTCTCTAATTACTGGATGGCCGGGGCCCAGCGGCTTCGGCTCGGCCTCCACGGCGGAGGACGTCACTCAAGGGATCGACGCCGCCGGCCTTACCGCCATTGTCACTGGTCATTTTCTGTTAACAAATCTTCTTCTTGAGAAAATGAAGAACACTGCAAAAAGCACAGGAATTGAGGGTAGAATTGTGAATTTGTCATCTATAGCTCATGTCCACACTTATGGAGATGGAATTCGCTTCAATAAACTCAATGATAAGAATGGGTATTCTGATAAAAGAGCATATGGGCAATCAAAATTAGCCAACATTTTACATGTCAAGGAGCTCACTCGTCGATTCCAAGAAGAGGGTGTGAACATAACGGCAAACGCGGTGCATCCAGGATTGATAATGACTCCTCTTATGAGGCATTCAATGCTTCTTATGCGGCTTTTGCAGGCTTTCACCTTCTTTATCTGGAAGAATGTCCCTCAGGGGGCATCGACAACATGCTACGTTGCGCTCCACCCGAGTTTGAAGGGAGTGAGTGGGAAATATTTTTTGGACAACAACGAGAAGCGACCGAGCTCCTATGCTAGAAATGGAAAACTTGCAAAGAAACTTTGGGACTTCAGCAAGGACTTGATAAGTTCAGTGTCTAAAGCATGAAAAAGAAGTGGCATTTAAACCTACGTTACCCCACAAAAACCTCAAGTCACATGCTAGCTCAACTTTATAAAGAATTAAGAGAGATTCAATAATGTGGTTCTTAAGAATGAAAAAATATTATAAATAGTTTTCTTATTAAAAGAGAGAGAGAGAGAGAGAGAAGATGAAGTGGTTTTGTGGACATTGGAAGCCCCTAGTGTTTGTGTGATTATTCCATTCATATTGTTTGATAATTATGATATTCAATCGATTACCTAAGTATATATGGTTTCTTTTTCCAA

Coding sequence (CDS)

ATGTCCATCATTTCTCTAATTACTGGATGGCCGGGGCCCAGCGGCTTCGGCTCGGCCTCCACGGCGGAGGACGTCACTCAAGGGATCGACGCCGCCGGCCTTACCGCCATTGTCACTGGTCATTTTCTGTTAACAAATCTTCTTCTTGAGAAAATGAAGAACACTGCAAAAAGCACAGGAATTGAGGGTAGAATTGTGAATTTGTCATCTATAGCTCATGTCCACACTTATGGAGATGGAATTCGCTTCAATAAACTCAATGATAAGAATGGGTATTCTGATAAAAGAGCATATGGGCAATCAAAATTAGCCAACATTTTACATGTCAAGGAGCTCACTCGTCGATTCCAAGAAGAGGGTGTGAACATAACGGCAAACGCGGTGCATCCAGGATTGATAATGACTCCTCTTATGAGGCATTCAATGCTTCTTATGCGGCTTTTGCAGGCTTTCACCTTCTTTATCTGGAAGAATGTCCCTCAGGGGGCATCGACAACATGCTACGTTGCGCTCCACCCGAGTTTGAAGGGAGTGAGTGGGAAATATTTTTTGGACAACAACGAGAAGCGACCGAGCTCCTATGCTAGAAATGGAAAACTTGCAAAGAAACTTTGGGACTTCAGCAAGGACTTGATAAGTTCAGTGTCTAAAGCATGA

Protein sequence

MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVTGHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRHSMLLMRLLQAFTFFIWKNVPQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLISSVSKA

Homology

[Close]

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 6.8e-63
Identity = 141/319 (44.20%), Postives = 173/319 (54.23%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           M I  ++TG  G SG+GSASTAEDVT  IDA  LTAI+T                     
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIAARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLL+KMK++A+ +GIEGRIVNLSSIAH +T
Sbjct: 121 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 216
           Y +GI F+ +ND + YS+K+AYGQSKLAN+LH   L+R+ QEEGVNIT N+VHPGLI T 
Sbjct: 181 YTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTN 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 4.4e-62
Identity = 140/319 (43.89%), Postives = 172/319 (53.92%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           M I  ++TG  G SG+GSASTAEDVT  IDA  LTAI+T                     
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIASRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLL+KMK++A+ +GIEGRIVNLSSIAH +T
Sbjct: 121 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 216
           Y +GI F+ +ND + YS+K+AYGQSKLAN+LH   L+R+ QEEGVNIT N+VHPGLI T 
Sbjct: 181 YTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTN 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.6e-51
Identity = 96/173 (55.49%), Postives = 131/173 (75.72%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLTNLLL+ MK T + +  EGRIVN++S AH   Y +GIRF+K+ND++ Y++ RAYG
Sbjct: 141 GHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKINDQSSYNNWRAYG 200

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRHSMLLMRLLQAFTFFIWKNV 159
           QSKLAN+LH  +LT+  +E+GVNITAN++HPG I+T L RH+  +  L+      + KNV
Sbjct: 201 QSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSAVNGLINVIGKLVLKNV 260

Query: 160 PQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLI 213
            QGA+TTCYVALHP +KGVSG+YF D+N  + + + ++  LAKKLWDFS +L+
Sbjct: 261 QQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLWDFSINLV 313

HSP 2 Score: 44.7 bits (104), Expect = 1.6e-03
Identity = 24/36 (66.67%), Postives = 25/36 (69.44%), Query Frame = 0


Query: 10 WP-----GPSGFGSASTAEDVTQGIDAAGLTAIVTG 41
          WP     G SGF  +STAE VT GIDA GLTAIVTG
Sbjct: 2  WPFSSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTG 37

[Close]

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 6.6e-50
Identity = 100/178 (56.18%), Postives = 133/178 (74.72%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLT LLL+ MK+T++ +  EGRIVNLSS AH  +Y +G+RF+K+NDK+ YS  RAYG
Sbjct: 140 GHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRFDKINDKSSYSSMRAYG 199

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRH-SMLLMRLLQAFTFFIWKN 159
           QSKL N+LH  ELT++ +E+GVNITAN++HPG IMT L R+ +  L   + A   +I K+
Sbjct: 200 QSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNPYLAVAVGAVAKYILKS 259

Query: 160 VPQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLISSVS 217
           VPQGA+TTCYVAL+P + GVSG+YF D+N  +P    ++ +LAKK+WDFS  L  S S
Sbjct: 260 VPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 317

HSP 2 Score: 44.3 bits (103), Expect = 2.1e-03
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 9  GWPGPSGFGSASTAEDVTQGIDAAGLTAIVTG 41
          G  G SGF S STAE+VT G+D  GLTAIVTG
Sbjct: 5  GSKGASGFSSRSTAEEVTHGVDGTGLTAIVTG 36

[Close]

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot
Match: Q8BYK4 (Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.8e-29
Identity = 79/173 (45.66%), Postives = 110/173 (63.58%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLT LLLE++K +A +     R+VNLSSIAH+      IRF+ L  +  Y    AYG
Sbjct: 150 GHFLLTYLLLERLKESAPA-----RVVNLSSIAHLI---GKIRFHDLQGQKRYCSAFAYG 209

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRHSMLLMRLLQAFTFFIWKNV 159
            SKLAN+L  +EL +R Q  G  +TA AVHPG++++ + R+S LL  L + F+ F +K+ 
Sbjct: 210 HSKLANLLFTRELAKRLQ--GTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPF-FKST 269

Query: 160 PQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLI 213
            QGA T+ + AL   L+ +SGKYF D      SS ARN K A++LW+ S +L+
Sbjct: 270 SQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSSRARNKKTAERLWNVSCELL 311

[Close]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL
Match: A0A1S3B6R4 (short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486400 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.7e-98
Identity = 206/321 (64.17%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAEDVTQGIDAA LTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAACLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKQQLLEENPTAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAH HT
Sbjct: 121 GVMFCPFQLSEDGIEMQFATNHIGHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHAHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+NDKNGYSDKRAYGQSKLANILHVKEL RRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGGGIRFNKINDKNGYSDKRAYGQSKLANILHVKELNRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL
Match: A0A0A0LD76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734860 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.8e-96
Identity = 201/321 (62.62%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAEDVTQGIDA  LTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAGCLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKQQLLEENPNAKLEVLKLDLSSIKSTTEFAHNFLHLNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLL+EKMKNTAKSTGIEGRIVNLSSIAH HT
Sbjct: 121 GVMFCPFQLSEDGIEMQFATNHLGHFLLTNLLIEKMKNTAKSTGIEGRIVNLSSIAHAHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+N+KNGYSDKRAYGQSKLANILHVKEL RRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGGGIRFNKINEKNGYSDKRAYGQSKLANILHVKELNRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL
Match: A0A6J1G2I1 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111450056 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.5e-94
Identity = 198/321 (61.68%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISL+TGWPGPSGFGSASTAE+V++GIDAA LTAIVT                     
Sbjct: 1   MSIISLVTGWPGPSGFGSASTAEEVSEGIDAANLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEGANKAKQQLLEQNPAAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMK+TAKSTGIEGRIVNLSSIAHVHT
Sbjct: 121 GVMFCPFQLSEDGIELQFATNHLGHFLLTNLLLEKMKSTAKSTGIEGRIVNLSSIAHVHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YGDGIRFNK+NDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGDGIRFNKINDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL
Match: A0A6J1F4L9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442120 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.3e-93
Identity = 197/321 (61.37%), Postives = 208/321 (64.80%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPG SGFGSASTAEDVTQGIDA+GLT IVT                     
Sbjct: 1   MSIISLITGWPGLSGFGSASTAEDVTQGIDASGLTVIVTGGASGIGLETVRVLAMRKAHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNVEAANKAKQQILQQNPSAKLDVLKLDLSSITSTTQFAQNFIALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVH+
Sbjct: 121 GVMFCPFQLSEDGIEMQFGTNHLGHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHS 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+N+K GYSDKRAYGQSKLANILH+KELTRRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGHGIRFNKINEKKGYSDKRAYGQSKLANILHIKELTRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL
Match: A0A6J1I6C3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111470041 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.9e-93
Identity = 197/321 (61.37%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAE+V++GIDAA LTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEEVSEGIDAANLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKHQLLEQNPAAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMK+TAKSTGIEGRIVNLSSIAHVHT
Sbjct: 121 GVMFCPFQLSEDGIELQFATNHLGHFLLTNLLLEKMKSTAKSTGIEGRIVNLSSIAHVHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           +GDGIRFNK+NDKNGYSDKRAYGQSKLANILHVKELTRRF+EEGVNITANAVHPGLIMTP
Sbjct: 181 FGDGIRFNKINDKNGYSDKRAYGQSKLANILHVKELTRRFKEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. NCBI nr
Match: XP_038903124.1 (short-chain dehydrogenase TIC 32 B, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 205/321 (63.86%), Postives = 213/321 (66.36%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAEDVTQGIDA GLTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDATGLTAIVTGGASGIGLETVRVLAKRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKLQLLGENPSAKLDVLKLDLSSIKSTREFAENFIALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAH HT
Sbjct: 121 GVMFCPFQLSEDGIEMQFATNHLGHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHAHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+N+KNGYSDKRAYGQSKLANILH+KELTR+FQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGGGIRFNKINEKNGYSDKRAYGQSKLANILHIKELTRQFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. NCBI nr
Match: XP_008442566.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo])

HSP 1 Score: 366.7 bits (940), Expect = 1.4e-97
Identity = 206/321 (64.17%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAEDVTQGIDAA LTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAACLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKQQLLEENPTAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAH HT
Sbjct: 121 GVMFCPFQLSEDGIEMQFATNHIGHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHAHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+NDKNGYSDKRAYGQSKLANILHVKEL RRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGGGIRFNKINDKNGYSDKRAYGQSKLANILHVKELNRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. NCBI nr
Match: XP_011651903.1 (short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN58884.1 hypothetical protein Csa_001749 [Cucumis sativus])

HSP 1 Score: 361.3 bits (926), Expect = 5.8e-96
Identity = 201/321 (62.62%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISLITGWPGPSGFGSASTAEDVTQGIDA  LTAIVT                     
Sbjct: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAGCLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKQQLLEENPNAKLEVLKLDLSSIKSTTEFAHNFLHLNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLL+EKMKNTAKSTGIEGRIVNLSSIAH HT
Sbjct: 121 GVMFCPFQLSEDGIEMQFATNHLGHFLLTNLLIEKMKNTAKSTGIEGRIVNLSSIAHAHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YG GIRFNK+N+KNGYSDKRAYGQSKLANILHVKEL RRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGGGIRFNKINEKNGYSDKRAYGQSKLANILHVKELNRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. NCBI nr
Match: XP_022945973.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata] >KAG7027961.1 Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354.0 bits (907), Expect = 9.3e-94
Identity = 198/321 (61.68%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISL+TGWPGPSGFGSASTAE+V++GIDAA LTAIVT                     
Sbjct: 1   MSIISLVTGWPGPSGFGSASTAEEVSEGIDAANLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEGANKAKQQLLEQNPAAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMK+TAKSTGIEGRIVNLSSIAHVHT
Sbjct: 121 GVMFCPFQLSEDGIELQFATNHLGHFLLTNLLLEKMKSTAKSTGIEGRIVNLSSIAHVHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YGDGIRFNK+NDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGDGIRFNKINDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. NCBI nr
Match: XP_023540240.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.0 bits (907), Expect = 9.3e-94
Identity = 198/321 (61.68%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           MSIISL+TGWPGPSGFGSASTAE+V++GIDAA LTAIVT                     
Sbjct: 1   MSIISLVTGWPGPSGFGSASTAEEVSEGIDAANLTAIVTGGASGIGLETVRVLAMRKVHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIGARNLEAANKAKQQLLEQNPAAKLDVLKLDLSSIKSTTEFAHNFLALNLPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLLEKMK+TAKSTGIEGRIVNLSSIAHVHT
Sbjct: 121 GVMFCPFQLSEDGIELQFATNHLGHFLLTNLLLEKMKSTAKSTGIEGRIVNLSSIAHVHT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           YGDGIRFNK+NDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP
Sbjct: 181 YGDGIRFNKINDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 240

[Close]

BLAST of Lsi03G020940 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.1e-73
Identity = 154/321 (47.98%), Postives = 187/321 (58.26%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           M + SLITG  GPSGFGSASTAE+VTQGIDA  LTAI+T                     
Sbjct: 1   MGLYSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VIGARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLL+ MKNTAK++G+EGRI+N+SS+AH++T
Sbjct: 121 GVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           Y +GI+F+ +ND   YSDKRAYGQSKLANILH  EL+R+ QEEGVNITAN+VHPGLI+T 
Sbjct: 181 YQEGIQFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTN 240

[Close]

BLAST of Lsi03G020940 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 1.5e-65
Identity = 142/321 (44.24%), Postives = 173/321 (53.89%), Query Frame = 0


Query: 1   MSIISLITGWPGPSGFGSASTAEDVTQGIDAAGLTAIVT--------------------- 60
           M I  ++TG  G SGFGSASTAEDVTQ IDA+ LTAI+T                     
Sbjct: 1   MGIYGVMTGKKGKSGFGSASTAEDVTQAIDASHLTAIITGGTSGIGLEAARVLAMRGAHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  IIAARNPKAANESKEMILQMNPNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINNA 120

Query: 121 -----------------------GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHT 180
                                  GHFLLTNLLL+KMK+TA+ +G++GRIVNLSSIAH +T
Sbjct: 121 GVMFCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTYT 180

Query: 181 YGDGIRFNKLNDKNGYSDKRAYGQSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTP 218
           Y +GI+F  +ND  GYS++RAYGQSKL+N+LH   L+RR QEEGVNIT N+VHPGL+ T 
Sbjct: 181 YSEGIKFQGINDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTN 240

[Close]

BLAST of Lsi03G020940 vs. TAIR 10
Match: AT4G23430.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 198.7 bits (504), Expect = 4.7e-51
Identity = 100/178 (56.18%), Postives = 133/178 (74.72%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLT LLL+ MK+T++ +  EGRIVNLSS AH  +Y +G+RF+K+NDK+ YS  RAYG
Sbjct: 140 GHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRFDKINDKSSYSSMRAYG 199

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRH-SMLLMRLLQAFTFFIWKN 159
           QSKL N+LH  ELT++ +E+GVNITAN++HPG IMT L R+ +  L   + A   +I K+
Sbjct: 200 QSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNPYLAVAVGAVAKYILKS 259

Query: 160 VPQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLISSVS 217
           VPQGA+TTCYVAL+P + GVSG+YF D+N  +P    ++ +LAKK+WDFS  L  S S
Sbjct: 260 VPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 317

HSP 2 Score: 44.3 bits (103), Expect = 1.5e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 9  GWPGPSGFGSASTAEDVTQGIDAAGLTAIVTG 41
          G  G SGF S STAE+VT G+D  GLTAIVTG
Sbjct: 5  GSKGASGFSSRSTAEEVTHGVDGTGLTAIVTG 36

[Close]

BLAST of Lsi03G020940 vs. TAIR 10
Match: AT4G11410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 4.0e-50
Identity = 94/169 (55.62%), Postives = 124/169 (73.37%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLTNLLLE+MK TA  +  EGRIV +SS  H   Y +G++F+K+ND+  Y+  +AYG
Sbjct: 140 GHFLLTNLLLERMKKTASESNREGRIVIVSSEGHRFAYREGVQFDKINDEARYNTLQAYG 199

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRHSMLLMRLLQAFTFFIWKNV 159
           QSKL NILH  EL R F+E+GVNITAN++HPG IMT L+R+   +  +  A   ++ K++
Sbjct: 200 QSKLGNILHATELARLFKEQGVNITANSLHPGSIMTNLLRYHSFINTIGNAVGKYVLKSI 259

Query: 160 PQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFS 209
           PQGA+TTCY ALHP  KGVSG+Y +DNN   P+S  ++  LAKKLW+FS
Sbjct: 260 PQGAATTCYAALHPQAKGVSGEYLMDNNISDPNSQGKDKDLAKKLWEFS 308

HSP 2 Score: 47.0 bits (110), Expect = 2.3e-05
Identity = 22/31 (70.97%), Postives = 24/31 (77.42%), Query Frame = 0


Query: 10 WPGPSGFGSASTAEDVTQGIDAAGLTAIVTG 41
          W G SGF + STAE+VT GID  GLTAIVTG
Sbjct: 6  WKGASGFSARSTAEEVTHGIDGTGLTAIVTG 36

[Close]

BLAST of Lsi03G020940 vs. TAIR 10
Match: AT4G23420.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 193.4 bits (490), Expect = 2.0e-49
Identity = 95/176 (53.98%), Postives = 131/176 (74.43%), Query Frame = 0


Query: 40  GHFLLTNLLLEKMKNTAKSTGIEGRIVNLSSIAHVHTYGDGIRFNKLNDKNGYSDKRAYG 99
           GHFLLT LLL+ MKNT++ +  EGRIVN+SS AH ++Y +G+RF+K+ND++ YS  RAYG
Sbjct: 140 GHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEGVRFDKINDESSYSSIRAYG 199

Query: 100 QSKLANILHVKELTRRFQEEGVNITANAVHPGLIMTPLMRH-SMLLMRLLQAFTFFIWKN 159
           QSKL N+LH  EL ++ +E+GVNITAN++HPG IMT L  + +  L   + A   ++ K+
Sbjct: 200 QSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGYFNSYLAGAVGAVAKYMVKS 259

Query: 160 VPQGASTTCYVALHPSLKGVSGKYFLDNNEKRPSSYARNGKLAKKLWDFSKDLISS 215
           VPQGA+TTCYVAL+P + GV+G+YF D+N  +P    ++ +LAKKLWDFS  L  S
Sbjct: 260 VPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTELAKKLWDFSTKLTDS 315

HSP 2 Score: 44.3 bits (103), Expect = 1.5e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 9  GWPGPSGFGSASTAEDVTQGIDAAGLTAIVTG 41
          G  G SGF S STAE+VT G+D  GLTAIVTG
Sbjct: 5  GSKGASGFSSRSTAEEVTHGVDGTGLTAIVTG 36

The following BLAST results are available for this feature:

BLAST of Lsi03G020940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078ISJ6	6.8e-63	44.20	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078IS66	4.4e-62	43.89	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
Q6RVV4	4.6e-51	55.49	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
A2RVM0	6.6e-50	56.18	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q8BYK4	3.8e-29	45.66	Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1	[more]

BLAST of Lsi03G020940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6R4	6.7e-98	64.17	short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0LD76	2.8e-96	62.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734860 PE=4 SV=1	[more]
A0A6J1G2I1	4.5e-94	61.68	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1F4L9	1.3e-93	61.37	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1I6C3	2.9e-93	61.37	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661...	[more]

BLAST of Lsi03G020940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903124.1	1.3e-98	63.86	short-chain dehydrogenase TIC 32 B, chloroplastic-like [Benincasa hispida]	[more]
XP_008442566.1	1.4e-97	64.17	PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo]	[more]
XP_011651903.1	5.8e-96	62.62	short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN58884.1 ...	[more]
XP_022945973.1	9.3e-94	61.68	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata] >KAG70...	[more]
XP_023540240.1	9.3e-94	61.68	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]

BLAST of Lsi03G020940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02540.1	1.1e-73	47.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	1.5e-65	44.24	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G23430.2	4.7e-51	56.18	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G11410.1	4.0e-50	55.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G23420.1	2.0e-49	53.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 13..215 e-value: 2.6E-40 score: 140.3
None	No IPR available	PANTHER	PTHR24320:SF229	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 3..40 coord: 40..214
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 40..214
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 3..40
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 38..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G020940.1	Lsi03G020940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity