Lsi03G020110 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G020110
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	membrane magnesium transporter
Location	chr03: 31524057 .. 31524728 (-)
RNA-Seq Expression	Lsi03G020110
Synteny	Lsi03G020110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGTTAATATCACAGAAGCAAGGCAGGTGATTGGAATTCCATAGATCAAGCTAAAAATTGGGACTTGTGGAGGTAAGCAAGGGGAAGATCCTTCTTCATTGCGTCACGTTGAAATTGGAAGTTTCTTCACCTCCACCGTCATTATCATCAACTCTGTAGCTTCCGCCGTTCGGTGATTGAGATCCGTCAGTCGAGATGGGAGTGGGCTTCGTGGTCGGTGTCTTCGGAGTTCTAATTCTGGCTCATGCTGCTTATTCCACCATCCAATGTATGCCCTAAAAAACCCATCATCATACTCTCTAATTCTAGCTTTACTTGCTTGTTTTTGTTGGTAAACCCTGCAGTTCTTGATTGATCTTGTTTTGACTGCAGATAGGAGCTTATTGAAGATCATGCAGGAAGAGTTTTCAGGCCCTCCGTTTCATGTATTTTCATCGCTCTTTTTTCTTTCTATAATCTTGTCTTTCAGCTGCTTCTACGTATCTCACTCGATTTGAGGTGTTCTTGAATTAAGGATTTTTTCTTTTCCCCTAATTTTAGGTGGCAATCGAGTTGATTCTAGGGTTAGTATTGTCCATGTGGGCTGCACTCACCGTACCAGGGAAGTTTCACTCGATAAATCCAGAAGCCGAGGAGAATAGGTACATTTTCCTGTCAAATTGTGCATGA

mRNA sequence

ATGCCGTTAATATCACAGAAGCAAGGCAGGTGATTGGAATTCCATAGATCAAGCTAAAAATTGGGACTTGTGGAGGTAAGCAAGGGGAAGATCCTTCTTCATTGCGTCACGTTGAAATTGGAAGTTTCTTCACCTCCACCGTCATTATCATCAACTCTGTAGCTTCCGCCGTTCGGTGATTGAGATCCGTCAGTCGAGATGGGAGTGGGCTTCGTGGTCGGTGTCTTCGGAGTTCTAATTCTGGCTCATGCTGCTTATTCCACCATCCAATATAGGAGCTTATTGAAGATCATGCAGGAAGAGTTTTCAGGCCCTCCGTTTCATGTGGCAATCGAGTTGATTCTAGGGTTAGTATTGTCCATGTGGGCTGCACTCACCGTACCAGGGAAGTTTCACTCGATAAATCCAGAAGCCGAGGAGAATAGGTACATTTTCCTGTCAAATTGTGCATGA

Coding sequence (CDS)

ATGGGAGTGGGCTTCGTGGTCGGTGTCTTCGGAGTTCTAATTCTGGCTCATGCTGCTTATTCCACCATCCAATATAGGAGCTTATTGAAGATCATGCAGGAAGAGTTTTCAGGCCCTCCGTTTCATGTGGCAATCGAGTTGATTCTAGGGTTAGTATTGTCCATGTGGGCTGCACTCACCGTACCAGGGAAGTTTCACTCGATAAATCCAGAAGCCGAGGAGAATAGGTACATTTTCCTGTCAAATTGTGCATGA

Protein sequence

MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALTVPGKFHSINPEAEENRYIFLSNCA

Homology

[Close]

BLAST of Lsi03G020110 vs. ExPASy Swiss-Prot
Match: Q2HIM5 (Membrane magnesium transporter OS=Arabidopsis thaliana OX=3702 GN=At5g03345 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 3.9e-27
Identity = 58/80 (72.50%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          M +GF+VGVFGVLIL+HAAYSTIQYR LLKIM+EEFS PP +V +ELI+GL L MWAALT
Sbjct: 1  MNLGFLVGVFGVLILSHAAYSTIQYRGLLKIMEEEFSRPPINVILELIIGLALCMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
           PGKF SI+P+++ENR +FL
Sbjct: 61 FPGKFLSIHPDSDENRAVFL 80

[Close]

BLAST of Lsi03G020110 vs. ExPASy Swiss-Prot
Match: Q86J02 (ER membrane protein complex subunit 5 homolog OS=Dictyostelium discoideum OX=44689 GN=mmgt PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.7e-04
Identity = 19/58 (32.76%), Postives = 31/58 (53.45%), Query Frame = 0


Query: 2  GVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAAL 60
          G+  +  V G+ I  H  YS +Q+R  L++  + F G PF + +E I+ L L  W  +
Sbjct: 4  GIAKIFCVIGISIFLHTVYSALQHRKYLRLTDQPFEGVPFDIVVECIISLFLFAWGII 61

[Close]

BLAST of Lsi03G020110 vs. ExPASy Swiss-Prot
Match: Q6TLE4 (ER membrane protein complex subunit 5 OS=Danio rerio OX=7955 GN=mmgt1 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.6e-04
Identity = 20/68 (29.41%), Postives = 42/68 (61.76%), Query Frame = 0


Query: 6  VVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALTVPGKF 65
          VVG+ G+  LAHAA+S  Q+RS +++ ++E    P  + ++ +L  V++ +  + + G+F
Sbjct: 9  VVGI-GLFALAHAAFSAAQHRSYMRLTEKENETLPIDIVLQTLLSFVITCYGIVHISGEF 68

Query: 66 HSINPEAE 74
            ++  +E
Sbjct: 69 KDMDASSE 75

[Close]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL
Match: A0A5D3DND5 (Membrane magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002680 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.6e-34
Identity = 77/80 (96.25%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL
Match: A0A1S3B730 (membrane magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103486496 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.6e-34
Identity = 77/80 (96.25%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL
Match: A0A0A0LFT4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740190 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.5e-34
Identity = 76/80 (95.00%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVA+ELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAVELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL
Match: A0A6J1GWV2 (membrane magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111457296 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.4e-32
Identity = 73/80 (91.25%), Postives = 77/80 (96.25%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPF+VAIEL+LGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFNVAIELVLGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKF SINPE+EENR + L
Sbjct: 61 VPGKFLSINPESEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL
Match: A0A6J1L020 (membrane magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111498628 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-31
Identity = 72/80 (90.00%), Postives = 77/80 (96.25%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEF+GPPF+VAIEL+LGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFAGPPFNVAIELVLGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKF SINPE+EENR + L
Sbjct: 61 VPGKFLSINPESEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. NCBI nr
Match: XP_038903501.1 (membrane magnesium transporter [Benincasa hispida])

HSP 1 Score: 154.1 bits (388), Expect = 5.5e-34
Identity = 77/80 (96.25%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. NCBI nr
Match: XP_008442703.1 (PREDICTED: membrane magnesium transporter [Cucumis melo] >KAA0043997.1 membrane magnesium transporter [Cucumis melo var. makuwa] >TYK25143.1 membrane magnesium transporter [Cucumis melo var. makuwa])

HSP 1 Score: 154.1 bits (388), Expect = 5.5e-34
Identity = 77/80 (96.25%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. NCBI nr
Match: XP_004137837.1 (membrane magnesium transporter [Cucumis sativus] >KGN58976.1 hypothetical protein Csa_000765 [Cucumis sativus])

HSP 1 Score: 153.7 bits (387), Expect = 7.1e-34
Identity = 76/80 (95.00%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVA+ELILGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAVELILGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKFHSINPEAEENR + L
Sbjct: 61 VPGKFHSINPEAEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. NCBI nr
Match: XP_022955639.1 (membrane magnesium transporter [Cucurbita moschata] >XP_022956379.1 membrane magnesium transporter [Cucurbita moschata] >XP_023538917.1 membrane magnesium transporter [Cucurbita pepo subsp. pepo] >XP_023538918.1 membrane magnesium transporter [Cucurbita pepo subsp. pepo] >KAG6596482.1 Membrane magnesium transporter, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028030.1 Membrane magnesium transporter [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 145.6 bits (366), Expect = 1.9e-31
Identity = 73/80 (91.25%), Postives = 77/80 (96.25%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPF+VAIEL+LGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFNVAIELVLGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKF SINPE+EENR + L
Sbjct: 61 VPGKFLSINPESEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. NCBI nr
Match: XP_023005713.1 (membrane magnesium transporter [Cucurbita maxima] >XP_023005714.1 membrane magnesium transporter [Cucurbita maxima])

HSP 1 Score: 144.4 bits (363), Expect = 4.3e-31
Identity = 72/80 (90.00%), Postives = 77/80 (96.25%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEF+GPPF+VAIEL+LGLVLSMWAALT
Sbjct: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFAGPPFNVAIELVLGLVLSMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
          VPGKF SINPE+EENR + L
Sbjct: 61 VPGKFLSINPESEENRIVSL 80

[Close]

BLAST of Lsi03G020110 vs. TAIR 10
Match: AT5G03345.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Magnesium transporter (InterPro:IPR018937); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 121.7 bits (304), Expect = 2.8e-28
Identity = 58/80 (72.50%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          M +GF+VGVFGVLIL+HAAYSTIQYR LLKIM+EEFS PP +V +ELI+GL L MWAALT
Sbjct: 1  MNLGFLVGVFGVLILSHAAYSTIQYRGLLKIMEEEFSRPPINVILELIIGLALCMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
           PGKF SI+P+++ENR +FL
Sbjct: 61 FPGKFLSIHPDSDENRAVFL 80

[Close]

BLAST of Lsi03G020110 vs. TAIR 10
Match: AT5G03345.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Magnesium transporter (InterPro:IPR018937); Has 155 Blast hits to 155 proteins in 63 species: Archae - 0; Bacteria - 0; Metazoa - 105; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 6 (source: NCBI BLink). )

HSP 1 Score: 115.2 bits (287), Expect = 2.6e-26
Identity = 56/80 (70.00%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1  MGVGFVVGVFGVLILAHAAYSTIQYRSLLKIMQEEFSGPPFHVAIELILGLVLSMWAALT 60
          M +GF+VGVFGVLIL+HAAYSTIQ   LLKIM+EEFS PP +V +ELI+GL L MWAALT
Sbjct: 1  MNLGFLVGVFGVLILSHAAYSTIQCIGLLKIMEEEFSRPPINVILELIIGLALCMWAALT 60

Query: 61 VPGKFHSINPEAEENRYIFL 81
           PGKF SI+P+++ENR +FL
Sbjct: 61 FPGKFLSIHPDSDENRAVFL 80

The following BLAST results are available for this feature:

BLAST of Lsi03G020110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2HIM5	3.9e-27	72.50	Membrane magnesium transporter OS=Arabidopsis thaliana OX=3702 GN=At5g03345 PE=3...	[more]
Q86J02	2.7e-04	32.76	ER membrane protein complex subunit 5 homolog OS=Dictyostelium discoideum OX=446...	[more]
Q6TLE4	3.6e-04	29.41	ER membrane protein complex subunit 5 OS=Danio rerio OX=7955 GN=mmgt1 PE=2 SV=1	[more]

BLAST of Lsi03G020110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DND5	2.6e-34	96.25	Membrane magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B730	2.6e-34	96.25	membrane magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103486496 PE=3 SV=1	[more]
A0A0A0LFT4	3.5e-34	95.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740190 PE=3 SV=1	[more]
A0A6J1GWV2	9.4e-32	91.25	membrane magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111457296 PE=...	[more]
A0A6J1L020	2.1e-31	90.00	membrane magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111498628 PE=3 ...	[more]

BLAST of Lsi03G020110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903501.1	5.5e-34	96.25	membrane magnesium transporter [Benincasa hispida]	[more]
XP_008442703.1	5.5e-34	96.25	PREDICTED: membrane magnesium transporter [Cucumis melo] >KAA0043997.1 membrane ...	[more]
XP_004137837.1	7.1e-34	95.00	membrane magnesium transporter [Cucumis sativus] >KGN58976.1 hypothetical protei...	[more]
XP_022955639.1	1.9e-31	91.25	membrane magnesium transporter [Cucurbita moschata] >XP_022956379.1 membrane mag...	[more]
XP_023005713.1	4.3e-31	90.00	membrane magnesium transporter [Cucurbita maxima] >XP_023005714.1 membrane magne...	[more]

BLAST of Lsi03G020110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03345.1	2.8e-28	72.50	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT5G03345.2	2.6e-26	70.00	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018937	Membrane magnesium transporter	PFAM	PF10270	MMgT	coord: 9..69 e-value: 2.5E-8 score: 34.2
None	No IPR available	PANTHER	PTHR21181	FAMILY NOT NAMED	coord: 3..76
None	No IPR available	PANTHER	PTHR21181:SF7	MEMBRANE MAGNESIUM TRANSPORTER 1	coord: 3..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G020110.1	Lsi03G020110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098655	cation transmembrane transport
cellular_component	GO:0005769	early endosome
cellular_component	GO:0072546	EMC complex
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022890	inorganic cation transmembrane transporter activity