Lsi03G019710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G019710
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease T(2)
Location	chr03: 31129341 .. 31131311 (+)
RNA-Seq Expression	Lsi03G019710
Synteny	Lsi03G019710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTTAGAAATTCTTGTACGCATAAACTTTTTGTTTTAAGGCATTTTGGTAGGTGAAGGCTTAGGCTGATTACTATTTTACCCATTTCATATATTTCTTCTTCCTAATATCATATAATTTTAATTTAAAATATATTTGCTATTTAGAAAGGGACATTGACAATAATGGTCAATGCCCCTTTTGCATTTTCTCATTGAAAAATGGATAAAAAAAAACCACCTTCCATTTTCTACGGTATGAGAGATGGGGGAAAAAAAAACCGCCTGCCATTTTCTACCATATTTTCAGAGATGTTTTCACCAAATATTAGTTTGTAGAACTTTTTAATTCTATTTCTTTATAAGAATTTGAGAGTTATAAACTTTGATTTCATACACATGGATAGATATTTAAAAAAAAAAAAAAAAAACAATTACAAAACCAACTAAATAATTCTTTTACAAAGTAATTATTCCAAGCGTTTAGTTTAACAATAATGTTAAAGTTAAATTATTCATTTATAAAGTAATAGTTACTAACTTATAAGAAAATCTATGTTAATTTCGTAATAATTTAAAGCGTGTTTGGTTTAACTTTTCAAGTGCTTAATTGTAAAAATAAATCATTTTGCAAAGAAATTAGATGGTTTGACAATCACATGAAATAGCTTTTCAAAAAAATTAAAATAGCTCTTGAAATACTTTAAAAGTTTATTTTAAAAATTTGTTTTAAATTGATATTTTCATCATCGATATGGATAGTTTAAACCAAGTGAGGATAGATTTTTTTTTAGAAAAAGAATAATAATAATAATAATAATAATAATAATAATAATAAAAAGAAGCTAATTATTACATGTATTTTGGCAAGGCAGAGTATACAAATTTGGGTAATAGGGCCAAGGTGTGTATATAAAAGGGTTAGCTTTCTTCATTCTCATTCATCTTCTTCCATCTGATAGTTGTATCAATTCTGAATGGCCCAACCTCTTTTCATTTTCTGTTTCTTAAGAGTTTGGTGTTGTTGGTGAATGGTGATCCATTTGATTTCTTCCAGTTTGTTCAACAATGGGGTCCAAACACATGCAATGACAGTCGTGGCATCCAGTGTTATGCTCAACCTCAACCCATGTTCACTATAAGTAATTATACCAATGTCCATCTTGTTTATCAAATGATTAAACCATCCATTTACAACTTCCGAGGTTCCTATCTTCTTCTTCAATATTTTTAAATTTTCTGTGTTTTTTTCCCTCTATCATTACCATTTTAATTTAACCAATGCTTATTTTTTTTTTTTTTTTTTTAGGTTACTGCAATTTTACCAGACCTAGACAAATATTGGCCAGATGTAATTGATGGGAATAACTCCAAATTTTGGACACATGAATGGGAAAATCACGGGGCATGTACAAGCCCTCCTTCTACCATATATCAATATTTCCAAATTTCTTTGAATCTCCGAACAAATAAACATTATGTTTTTTTTTTTTTTAATATACGTGAGTATCCGGGCTAGCTTACGCGCACCTCAACTAGTCTCACAGGACAACCCACCCGACCCTACAACAGGCGAGTTGTCACTTACCCGTAGGATATTAAATTCTAGATAGATGACCACCATAGATTGAACCCACAACTCCTTAGCCATTTAGCTTATTTTTTTAGATGTTCCTATACTACCATTAGGTCAATCCATGATGGTTGATTTGTTGGCAATCTTAAACGCGGCGGGGCTGAGCCTCAAAATCTCCAAAGACCTTGTATCCCAAGACATACTAAGCCCCATTCAGAATAAAACTGGAAAGATGCCCGGCATCCACTGCAACAACCATGCTAAAAGTTGCAATTGTATGAAATTGTTGTGTGTTTTGGAAAGGATGGTACTACCCTCCCTTTGTTAGCAACAGTTGCAAATCTAAATTTGTGTGGCTCCTCCCACAATACAATGTAGTTACATTGGTATCCCTGATTATCCATGAGTCATGA

mRNA sequence

ATGAAAAGTTTGGTGTTGTTGGTGAATGGTGATCCATTTGATTTCTTCCAGTTTGTTCAACAATGGGGTCCAAACACATGCAATGACAGTCGTGGCATCCAGTGTTATGCTCAACCTCAACCCATGTTCACTATAAGTAATTATACCAATGTCCATCTTGTTACTGCAATTTTACCAGACCTAGACAAATATTGGCCAGATGTAATTGATGGGAATAACTCCAAATTTTGGACACATGAATGGGAAAATCACGGGGCATGTACAAGCCCTCCTTCTACCATATATCAATATTTCCAAATTTCTTTGAATCTCCGAACAAATAAACATTATGACAACCCACCCGACCCTACAACAGGCGAGTTGTCACTTACCCATGTTCCTATACTACCATTAGGTCAATCCATGATGGTTGATTTGTTGGCAATCTTAAACGCGGCGGGGCTGAGCCTCAAAATCTCCAAAGACCTTGTATCCCAAGACATACTAAGCCCCATTCAGAATAAAACTGGAAAGATGCCCGGCATCCACTGCAACAACCATGCTAAAAGATGGTACTACCCTCCCTTTGTTAGCAACAGTTGCAAATCTAAATTTGTGTGGCTCCTCCCACAATACAATGTAGTTACATTGGTATCCCTGATTATCCATGAGTCATGA

Coding sequence (CDS)

ATGAAAAGTTTGGTGTTGTTGGTGAATGGTGATCCATTTGATTTCTTCCAGTTTGTTCAACAATGGGGTCCAAACACATGCAATGACAGTCGTGGCATCCAGTGTTATGCTCAACCTCAACCCATGTTCACTATAAGTAATTATACCAATGTCCATCTTGTTACTGCAATTTTACCAGACCTAGACAAATATTGGCCAGATGTAATTGATGGGAATAACTCCAAATTTTGGACACATGAATGGGAAAATCACGGGGCATGTACAAGCCCTCCTTCTACCATATATCAATATTTCCAAATTTCTTTGAATCTCCGAACAAATAAACATTATGACAACCCACCCGACCCTACAACAGGCGAGTTGTCACTTACCCATGTTCCTATACTACCATTAGGTCAATCCATGATGGTTGATTTGTTGGCAATCTTAAACGCGGCGGGGCTGAGCCTCAAAATCTCCAAAGACCTTGTATCCCAAGACATACTAAGCCCCATTCAGAATAAAACTGGAAAGATGCCCGGCATCCACTGCAACAACCATGCTAAAAGATGGTACTACCCTCCCTTTGTTAGCAACAGTTGCAAATCTAAATTTGTGTGGCTCCTCCCACAATACAATGTAGTTACATTGGTATCCCTGATTATCCATGAGTCATGA

Protein sequence

MKSLVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTISNYTNVHLVTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLRTNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPIQNKTGKMPGIHCNNHAKRWYYPPFVSNSCKSKFVWLLPQYNVVTLVSLIIHES

Homology

[Close]

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-11
Identity = 34/112 (30.36%), Postives = 54/112 (48.21%), Query Frame = 0


Query: 13  FDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI---------SNYTN--------VHLVT 72
           FD F FVQQW P  C+  +   C       FTI         ++ TN        +  ++
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 73  AILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLRTN 108
            +   L+  WP+V+  NN +FW+HEW  HG C+        YF++++++R N
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNN 112

[Close]

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 60/224 (26.79%), Postives = 87/224 (38.84%), Query Frame = 0


Query: 4   LVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCY---AQPQPMFTI----SNY-------- 63
           LVL V    FDFF FVQQW  + C D+R   CY    +P   F+I     NY        
Sbjct: 19  LVLCVTSQDFDFFYFVQQWPASYC-DTRRSCCYPTTGKPDEDFSIHGLWPNYKDGKWPQN 78

Query: 64  ------TNVHLVTAILPDLDKYWPDVI--DGNNSKFWTHEWENHGACTSPPSTIYQYFQI 123
                  +    + ++  ++K WP +     +  KFW+HEW  HG C++     + YFQ 
Sbjct: 79  CDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTCSA--LNQHAYFQT 138

Query: 124 SLNLRTNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQD 183
           +L+ +T                               +LL  LN AG+  +       + 
Sbjct: 139 ALDFKTKS-----------------------------NLLQNLNNAGIKPRNGDYYGVES 198

Query: 184 ILSPIQNKTGKMPGIHCN-----NHAKRWYYPPFVSNSCKSKFV 200
           I   I+   G  P I CN     NH  + Y      +S  SKF+
Sbjct: 199 IKKAIEKGVGHTPFIECNVDSQGNH--QLYQVYLCVDSSASKFI 208

[Close]

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 38/120 (31.67%), Postives = 56/120 (46.67%), Query Frame = 0


Query: 9   NGDPFDFFQFVQQWGPNTCNDSRGIQCY---AQPQPMFTI----SNYT------------ 68
           + + FDFF FVQQW  + C D++   CY    +P   F I     NY             
Sbjct: 26  SSEDFDFFYFVQQWPGSYC-DTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASK 85

Query: 69  --NVHLVTAILPDLDKYWPDVI--DGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 106
             +   ++ +L  + K WP +    G+   FW HEWE HG C+      ++YFQ +LNL+
Sbjct: 86  PFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLK 144

[Close]

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.1e-08
Identity = 38/120 (31.67%), Postives = 54/120 (45.00%), Query Frame = 0


Query: 4   LVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-------------SNYTN 63
           L+L  +   +D+FQF QQ+ P  CN S+   C   P  +FT+              N TN
Sbjct: 19  LILSSSTVGYDYFQFTQQYQPAVCN-SKPTPCKDPPDKLFTVHGLWPSNLNGPHPENCTN 78

Query: 64  V----HLVTAILPDLDKYWPDVID-GNNSKFWTHEWENHGACTSPP-STIYQYFQISLNL 105
                H +  I   L   WP+V+D  N+  FW  +W  HG+C  P       YFQ  +N+
Sbjct: 79  ATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMNDTHYFQTVINM 137

[Close]

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot
Match: B8XY56 (Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.7e-08
Identity = 29/95 (30.53%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 13  FDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTISNYTNVHLVTAILPDLDKYWPDVIDGN 72
           F ++     W PNT     G++C          S + N  L+  ILP+++K+WPD+++ +
Sbjct: 50  FSYWTLHGLW-PNT-----GVRC--------NTSWHFNASLIEDILPEMEKFWPDLLEPS 109

Query: 73  NSKFWTHEWENHGACTSPPSTI---YQYFQISLNL 105
           + KFW +EW  HG C +   ++   ++YF  +L L
Sbjct: 110 SPKFWNYEWTKHGTCAAKSESLNSEHKYFGKALEL 130

[Close]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.8e-43
Identity = 110/245 (44.90%), Postives = 130/245 (53.06%), Query Frame = 0


Query: 3   SLVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHL---- 62
           S +L VNG PFDFFQ VQQWGPN C+ + G +CYAQPQPMFTI     SN++ V +    
Sbjct: 12  SSLLFVNGIPFDFFQLVQQWGPNICSPA-GSKCYAQPQPMFTIHGLWPSNFSTVPILCPR 71

Query: 63  ---------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNL 122
                    VT++   L+KYWPDVI G NS FW HEW  H  C  PP TI+ YF ISL  
Sbjct: 72  TKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTIFWYFDISLRH 131

Query: 123 RTNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSP 182
           R NK Y                           DLL ILN AGLS +  KDL+SQ++L P
Sbjct: 132 RMNKTY---------------------------DLLTILNDAGLSHQTHKDLISQNVLDP 191

Query: 183 IQNKTGKMPGIHCNNH--AKRWYY-----------------PPFVSNSCKS--KFVWLLP 209
           I+N TG  PGI CN +   K+                    P FVSN+C S  KFVWLLP
Sbjct: 192 IKNATGMEPGIRCNINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLP 228

[Close]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 6.6e-37
Identity = 101/241 (41.91%), Postives = 121/241 (50.21%), Query Frame = 0


Query: 3   SLVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHL---- 62
           SL+L VNG PFDFFQ V+QWGPNTCND  G QC+AQPQP FTI     SN++N  L    
Sbjct: 12  SLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPSNFSNARLFCAT 71

Query: 63  --------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 122
                   ++ +  DLD+YWPDVI+GNN  FW++EW+ HGAC  PP  I QYFQ+SL+  
Sbjct: 72  RKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLD-- 131

Query: 123 TNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLK--ISKDLVSQDILS 182
                                           DLL ILN      +   +  L  +DIL+
Sbjct: 132 ---------------------------HHRTYDLLKILNGVWHRNRQMPNNGLAPKDILN 191

Query: 183 PIQNKTGKMPGIHC-------------------NNHAKRWYYPPFVSNSC-KSKFVWLLP 205
           PI N T K  GI C                   NN       P FVSNSC + KFVWLLP
Sbjct: 192 PIFNATNKTAGIRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLP 221

[Close]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 6.2e-35
Identity = 93/234 (39.74%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 3   SLVLLVNGD-PFDFFQFVQQWGPNTCN-DSRGIQCYAQPQPMFTI-----SNYTNVHL-- 62
           SLV+ VNG  PF+FFQFV+QWGPN CN  +R   C+  PQ  FTI     SN++N HL  
Sbjct: 12  SLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLC 71

Query: 63  --------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 122
                   V ++   L+  WPD+  GNN KFW HEW+ HG C+ PP +++QYFQISLNL 
Sbjct: 72  QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLL 131

Query: 123 TNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPI 182
                                           DLL IL AAGL+ + S++L  Q+I++PI
Sbjct: 132 RK-----------------------------FDLLTILKAAGLNPQTSQNLAIQNIMAPI 191

Query: 183 QNKTGKMPGIHCNNHAKRWY-------------------YPPFVSNSCKSKFVW 201
           Q  T K PGI CN + K                       P FVSN+C   FVW
Sbjct: 192 QRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.2e-32
Identity = 91/235 (38.72%), Postives = 116/235 (49.36%), Query Frame = 0


Query: 13  FDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHLVTA----------- 72
           FD+FQ VQQW P TC+   G++CYA P  MFTI     SNY+NV LV A           
Sbjct: 24  FDYFQMVQQWPPATCS---GVRCYANPPAMFTIHGLWPSNYSNVPLVCAGQPFNPAQIAT 83

Query: 73  ILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLRTNKHYDNPPDP 132
           ++P L+ YWPDVI GNN +FW HEW++HG C+ PP    QYFQ +LN+RTN +Y      
Sbjct: 84  LVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNY------ 143

Query: 133 TTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPIQNKTGKMPGIH 192
                                DLLAILN AGL    +       I   IQ  TGK PG+ 
Sbjct: 144 ---------------------DLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLR 203

Query: 193 CNNHA---KRWYY----------------PPFVSNSCKSKFVWL-LPQYNVVTLV 212
           CN +A   K+  +                  F   +C S+FVWL  P  ++V+ V
Sbjct: 204 CNKNAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWLHRPPLSLVSAV 228

[Close]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.9e-32
Identity = 93/236 (39.41%), Postives = 115/236 (48.73%), Query Frame = 0


Query: 3   SLVLLVNG--DPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHLVT 62
           +LVLL  G    FD+FQ VQQW P TC+   G++CYA P  MFTI     S Y+NV  V 
Sbjct: 13  NLVLLTIGVKAQFDYFQMVQQWPPATCS---GVRCYANPPAMFTIHGLWPSTYSNVPPVC 72

Query: 63  A-----------ILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNL 122
           A           + P L+ YWPDVI+GNN +FW HEW+NHG C+ PP    QYFQ +LN+
Sbjct: 73  AGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYFQTTLNI 132

Query: 123 RTNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSP 182
           RTN +Y                           DLLAILN AGL    +     Q I   
Sbjct: 133 RTNHNY---------------------------DLLAILNTAGLGPTATTTRQYQAIEGA 192

Query: 183 IQNKTGKMPGIHCNNHA----KRWY---------------YPPFVSNSCKSKFVWL 202
           IQ  TGK PG+ CN +A    K+ +                  F   +C S+FVWL
Sbjct: 193 IQAATGKKPGLRCNKNAQSKRKQLFEIILCFDKNGVTLINCTQFAGITCPSQFVWL 218

[Close]

BLAST of Lsi03G019710 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 185.3 bits (469), Expect = 5.7e-43
Identity = 110/245 (44.90%), Postives = 130/245 (53.06%), Query Frame = 0


Query: 3   SLVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHL---- 62
           S +L VNG PFDFFQ VQQWGPN C+ + G +CYAQPQPMFTI     SN++ V +    
Sbjct: 12  SSLLFVNGIPFDFFQLVQQWGPNICSPA-GSKCYAQPQPMFTIHGLWPSNFSTVPILCPR 71

Query: 63  ---------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNL 122
                    VT++   L+KYWPDVI G NS FW HEW  H  C  PP TI+ YF ISL  
Sbjct: 72  TKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTIFWYFDISLRH 131

Query: 123 RTNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSP 182
           R NK Y                           DLL ILN AGLS +  KDL+SQ++L P
Sbjct: 132 RMNKTY---------------------------DLLTILNDAGLSHQTHKDLISQNVLDP 191

Query: 183 IQNKTGKMPGIHCNNH--AKRWYY-----------------PPFVSNSCKS--KFVWLLP 209
           I+N TG  PGI CN +   K+                    P FVSN+C S  KFVWLLP
Sbjct: 192 IKNATGMEPGIRCNINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLP 228

[Close]

BLAST of Lsi03G019710 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 164.1 bits (414), Expect = 1.4e-36
Identity = 101/241 (41.91%), Postives = 121/241 (50.21%), Query Frame = 0


Query: 3   SLVLLVNGDPFDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHL---- 62
           SL+L VNG PFDFFQ V+QWGPNTCND  G QC+AQPQP FTI     SN++N  L    
Sbjct: 12  SLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPSNFSNARLFCAT 71

Query: 63  --------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 122
                   ++ +  DLD+YWPDVI+GNN  FW++EW+ HGAC  PP  I QYFQ+SL+  
Sbjct: 72  RKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLD-- 131

Query: 123 TNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLK--ISKDLVSQDILS 182
                                           DLL ILN      +   +  L  +DIL+
Sbjct: 132 ---------------------------HHRTYDLLKILNGVWHRNRQMPNNGLAPKDILN 191

Query: 183 PIQNKTGKMPGIHC-------------------NNHAKRWYYPPFVSNSC-KSKFVWLLP 205
           PI N T K  GI C                   NN       P FVSNSC + KFVWLLP
Sbjct: 192 PIFNATNKTAGIRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLP 221

[Close]

BLAST of Lsi03G019710 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 157.5 bits (397), Expect = 1.3e-34
Identity = 93/234 (39.74%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 3   SLVLLVNGD-PFDFFQFVQQWGPNTCN-DSRGIQCYAQPQPMFTI-----SNYTNVHL-- 62
           SLV+ VNG  PF+FFQFV+QWGPN CN  +R   C+  PQ  FTI     SN++N HL  
Sbjct: 12  SLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLC 71

Query: 63  --------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 122
                   V ++   L+  WPD+  GNN KFW HEW+ HG C+ PP +++QYFQISLNL 
Sbjct: 72  QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLL 131

Query: 123 TNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPI 182
                                           DLL IL AAGL+ + S++L  Q+I++PI
Sbjct: 132 RK-----------------------------FDLLTILKAAGLNPQTSQNLAIQNIMAPI 191

Query: 183 QNKTGKMPGIHCNNHAKRWY-------------------YPPFVSNSCKSKFVW 201
           Q  T K PGI CN + K                       P FVSN+C   FVW
Sbjct: 192 QRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of Lsi03G019710 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 157.5 bits (397), Expect = 1.3e-34
Identity = 93/234 (39.74%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 3   SLVLLVNGD-PFDFFQFVQQWGPNTCN-DSRGIQCYAQPQPMFTI-----SNYTNVHL-- 62
           SLV+ VNG  PF+FFQFV+QWGPN CN  +R   C+  PQ  FTI     SN++N HL  
Sbjct: 12  SLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSNHSNAHLLC 71

Query: 63  --------VTAILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 122
                   V ++   L+  WPD+  GNN KFW HEW+ HG C+ PP +++QYFQISLNL 
Sbjct: 72  QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLL 131

Query: 123 TNKHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPI 182
                                           DLL IL AAGL+ + S++L  Q+I++PI
Sbjct: 132 RK-----------------------------FDLLTILKAAGLNPQTSQNLAIQNIMAPI 191

Query: 183 QNKTGKMPGIHCNNHAKRWY-------------------YPPFVSNSCKSKFVW 201
           Q  T K PGI CN + K                       P FVSN+C   FVW
Sbjct: 192 QRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of Lsi03G019710 vs. NCBI nr
Match: XP_022932791.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 149.1 bits (375), Expect = 4.6e-32
Identity = 91/235 (38.72%), Postives = 116/235 (49.36%), Query Frame = 0


Query: 13  FDFFQFVQQWGPNTCNDSRGIQCYAQPQPMFTI-----SNYTNVHLVTA----------- 72
           FD+FQ VQQW P TC+   G++CYA P  MFTI     SNY+NV LV A           
Sbjct: 24  FDYFQMVQQWPPATCS---GVRCYANPPAMFTIHGLWPSNYSNVPLVCAGQPFNPAQIAT 83

Query: 73  ILPDLDKYWPDVIDGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLRTNKHYDNPPDP 132
           ++P L+ YWPDVI GNN +FW HEW++HG C+ PP    QYFQ +LN+RTN +Y      
Sbjct: 84  LVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNY------ 143

Query: 133 TTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPIQNKTGKMPGIH 192
                                DLLAILN AGL    +       I   IQ  TGK PG+ 
Sbjct: 144 ---------------------DLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLR 203

Query: 193 CNNHA---KRWYY----------------PPFVSNSCKSKFVWL-LPQYNVVTLV 212
           CN +A   K+  +                  F   +C S+FVWL  P  ++V+ V
Sbjct: 204 CNKNAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWLHRPPLSLVSAV 228

[Close]

BLAST of Lsi03G019710 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 38/120 (31.67%), Postives = 56/120 (46.67%), Query Frame = 0


Query: 9   NGDPFDFFQFVQQWGPNTCNDSRGIQCY---AQPQPMFTI----SNYT------------ 68
           + + FDFF FVQQW  + C D++   CY    +P   F I     NY             
Sbjct: 26  SSEDFDFFYFVQQWPGSYC-DTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASK 85

Query: 69  --NVHLVTAILPDLDKYWPDVI--DGNNSKFWTHEWENHGACTSPPSTIYQYFQISLNLR 106
             +   ++ +L  + K WP +    G+   FW HEWE HG C+      ++YFQ +LNL+
Sbjct: 86  PFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLK 144

[Close]

BLAST of Lsi03G019710 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.7e-08
Identity = 50/196 (25.51%), Postives = 76/196 (38.78%), Query Frame = 0


Query: 11  DPFDFFQFVQQWGPNTCNDSRGIQCY---AQPQPMFTI----SNYT-------------- 70
           + FDFF FV QW    C+ SR   CY    +P   F I     NY               
Sbjct: 26  EDFDFFYFVLQWPGAYCDTSRAC-CYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQF 85

Query: 71  NVHLVTAILPDLDKYWPDVIDGNNS--KFWTHEWENHGACTSPPSTIYQYFQISLNLRTN 130
           +   ++ ++  L K WP +   +N    FW HEWE HG C+      ++YF+ +L L+  
Sbjct: 86  DRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLK-- 145

Query: 131 KHYDNPPDPTTGELSLTHVPILPLGQSMMVDLLAILNAAGLSLKISKDLVSQDILSPIQN 184
                                         +LL IL  +G++       + + I + I++
Sbjct: 146 ---------------------------QKANLLQILKNSGINPDDGFYNLDK-ITNAIKD 190

[Close]

BLAST of Lsi03G019710 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 53.5 bits (127), Expect = 2.4e-07
Identity = 23/59 (38.98%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 54  VTAILPDLDKYWPDV-------IDGNNSKFWTHEWENHGACTSPP-STIYQYFQISLNL 105
           ++ ++  L+KYWP +        +G    FW HEWE HG C+SP     Y YF  +LNL
Sbjct: 96  ISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNL 154

The following BLAST results are available for this feature:

BLAST of Lsi03G019710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.6e-11	30.36	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80196	1.1e-09	26.79	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42813	1.5e-09	31.67	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
O80325	2.1e-08	31.67	Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
B8XY56	3.7e-08	30.53	Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 PE=2 SV=1	[more]

BLAST of Lsi03G019710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAB0	2.8e-43	44.90	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A5D3DP91	6.6e-37	41.91	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A0A0LAN6	6.2e-35	39.74	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1EXD5	2.2e-32	38.72	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1IAR7	2.9e-32	39.41	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]

BLAST of Lsi03G019710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651927.1	5.7e-43	44.90	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
TYK25090.1	1.4e-36	41.91	S-RNase [Cucumis melo var. makuwa]	[more]
KAE8651058.1	1.3e-34	39.74	hypothetical protein Csa_002465 [Cucumis sativus]	[more]
XP_031738690.1	1.3e-34	39.74	ribonuclease MC-like [Cucumis sativus]	[more]
XP_022932791.1	4.6e-32	38.72	ribonuclease MC-like [Cucurbita moschata]	[more]

BLAST of Lsi03G019710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.1e-10	31.67	ribonuclease 1	[more]
AT1G14220.1	3.7e-08	25.51	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.4e-07	38.98	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 53..107 e-value: 6.8E-13 score: 49.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 12..180
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 7..197 e-value: 7.6E-25 score: 90.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 13..107
None	No IPR available	CDD	cd00374	RNase_T2	coord: 13..201 e-value: 7.07402E-26 score: 97.5267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G019710.1	Lsi03G019710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding