Lsi03G018580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G018580
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr03: 30010097 .. 30012116 (-)
RNA-Seq Expression	Lsi03G018580
Synteny	Lsi03G018580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTGTTTCATCTTCAAACTTCTTTCTATTATAAATTTACAATCCCCACCCCCCATCTAATCAATACGCTCTCTCCTCTCTCTCTCTCTCTCTCTCCCTAAAAGAAGAAGAAGAAATGACAAGCACTGAGGTTATCTCTACAACAACAGGGCCAAGGAGGTAATAATTCTCACGAATATGTGACTAATTTTTTTATACTTTTAAACTCTCTTTATTTCTTCTTATATCTTTCTCTGTTTATATATATATATATATAAGGTAGATCTATGAAGAGGCCAGGAAGCACATGCCCTCTTCAAATAGTGACATTTTACATATATGTATATTGATTTTCATTAAATATTAAAAATTTCTAGTTATACCTTAATATATAGTTTCTTTCAAATATACAATCACTAAAAAATGACCCAGTGGCGTATAGGAGTATTAGTTTTCTTCAATTATCTCAAAGTTGAGAAATCGAGTCCTACAACGACACCATGTAATTAGACTATAGCAACCAAGTATTTATCTTTAAGTTATAAGTTTAGTTCATAAACTTCTAAGTTTGTGTACGTTAGATCTCGTTAAAAGTGTATAATTTAATTCTTTAATTTTTAATTTTGTGTCTAATAAATTTATTTTTCTTTGTCCCATAAGTCCCTTTGTTTTGTGTCCATAAGGTCCTCGATCAATGTGATATTTTATATAGGGTCTATTTTAGGGTCTCATTAGACATAAAATTGAATTTTAAATCTAATAGATTAATCAATTTTCGAATGTGTAAGAAAGACAAAATTAAAAATTCAACAACTAATTATAACTATTTTTAAACTTCATAAACTTACATAAAATTAACATTTTACAAACCTACTAGCGTGCTTTTCTAAAGTTAAACTCAATTATAACCACTTATGTTTTATAGACTAAACTTATAATTTAACTTATACTTTTCTCCCTCTTATCACTAAAATGTTTAGTAGCCGACAATAGACAAATCACTGTTATAAGATCATCTTCTTTCGTCCAAAATCAAGCTTTTATTCTTTTCTTTGGTTCATATTCTGTCAACCACTCTTAACAACATATGTCCCTTTTGTGTGTTCCATCAGGCTAGAGGGAAAAGTAGCCTTAATAACCGGCGGTGCAAGTGGGATCGGCGAATGCACAGCAAAGCTCTTCGTTCACCACGGCGCCAAAGTAGTTATCGCCGACATCCAAGATGACCTCGGTCATGCACTTTGTGCCACCATACTTGGCCCAACCAACTCCCTCTATATTCACTGTGACGTCACAGACGAATCCCAAGTCCAAGCCGCGGTGGTAGCCGCCGTCGAAACCTTCGGCAAGCTCGACATCATGATGAACAATGCCGGCATAGTCGACCCTAGCAAGCCCCGAATCATCGACAATGAAAAACATGACTTCGACCGAGTCCTCAGCATTAATATCACTGGTGTCTTCCTTGGCATCAAACACGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCAATTATATCGACTGCGAGTGTGGCATCGTATGTCGGTGGCTCTGCGTCGCATGCTTACACATGCTCGAAGCATGCAGTGGTGGGGTTGACAAAAAATGCGGCGGTAGAATTAGGGCAGTTTGGGATTAGGGTGAATTGCTTGTCGCCTTATGCATTGGCGACACCGTTGGCAACGAGGTTTGTGGGGTTGGAGGATGAGGAGTTTGAGATGGCTATAAACTCTATGGCGAATCTAAAAGGTGTCACGCTTAAGGCTGAGGATGTAGCCAATGCTGCTTTATTTTTTGCCAGTGATGAAAGTAGGTATGTGAGTGGCCACAATTTGTTGATTGATGGAGGCTTTAGTATTGTTAATCCTAATATAAAAAATTTTCAATACCCTCAACATTAGGCGCTTTGTCTCGAATAAATTATTGGAGAAAATCATTAGTTATGATACTAGCTTTTTTCTCTTACCTATTGTTTGTGTTTTTTTTCCTAACCTTTTTTTCTCCATGGAATTTGTGTAATGACTCGGGCTTTCTCCC

mRNA sequence

TTTTGTTTCATCTTCAAACTTCTTTCTATTATAAATTTACAATCCCCACCCCCCATCTAATCAATACGCTCTCTCCTCTCTCTCTCTCTCTCTCTCCCTAAAAGAAGAAGAAGAAATGACAAGCACTGAGGTTATCTCTACAACAACAGGGCCAAGGAGGCTAGAGGGAAAAGTAGCCTTAATAACCGGCGGTGCAAGTGGGATCGGCGAATGCACAGCAAAGCTCTTCGTTCACCACGGCGCCAAAGTAGTTATCGCCGACATCCAAGATGACCTCGGTCATGCACTTTGTGCCACCATACTTGGCCCAACCAACTCCCTCTATATTCACTGTGACGTCACAGACGAATCCCAAGTCCAAGCCGCGGTGGTAGCCGCCGTCGAAACCTTCGGCAAGCTCGACATCATGATGAACAATGCCGGCATAGTCGACCCTAGCAAGCCCCGAATCATCGACAATGAAAAACATGACTTCGACCGAGTCCTCAGCATTAATATCACTGGTGTCTTCCTTGGCATCAAACACGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCAATTATATCGACTGCGAGTGTGGCATCGTATGTCGGTGGCTCTGCGTCGCATGCTTACACATGCTCGAAGCATGCAGTGGTGGGGTTGACAAAAAATGCGGCGGTAGAATTAGGGCAGTTTGGGATTAGGGTGAATTGCTTGTCGCCTTATGCATTGGCGACACCGTTGGCAACGAGGTTTGTGGGGTTGGAGGATGAGGAGTTTGAGATGGCTATAAACTCTATGGCGAATCTAAAAGGTGTCACGCTTAAGGCTGAGGATGTAGCCAATGCTGCTTTATTTTTTGCCAGTGATGAAAGTAGGTATGTGAGTGGCCACAATTTGTTGATTGATGGAGGCTTTAGTATTGTTAATCCTAATATAAAAAATTTTCAATACCCTCAACATTAGGCGCTTTGTCTCGAATAAATTATTGGAGAAAATCATTAGTTATGATACTAGCTTTTTTCTCTTACCTATTGTTTGTGTTTTTTTTCCTAACCTTTTTTTCTCCATGGAATTTGTGTAATGACTCGGGCTTTCTCCC

Coding sequence (CDS)

ATGACAAGCACTGAGGTTATCTCTACAACAACAGGGCCAAGGAGGCTAGAGGGAAAAGTAGCCTTAATAACCGGCGGTGCAAGTGGGATCGGCGAATGCACAGCAAAGCTCTTCGTTCACCACGGCGCCAAAGTAGTTATCGCCGACATCCAAGATGACCTCGGTCATGCACTTTGTGCCACCATACTTGGCCCAACCAACTCCCTCTATATTCACTGTGACGTCACAGACGAATCCCAAGTCCAAGCCGCGGTGGTAGCCGCCGTCGAAACCTTCGGCAAGCTCGACATCATGATGAACAATGCCGGCATAGTCGACCCTAGCAAGCCCCGAATCATCGACAATGAAAAACATGACTTCGACCGAGTCCTCAGCATTAATATCACTGGTGTCTTCCTTGGCATCAAACACGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCAATTATATCGACTGCGAGTGTGGCATCGTATGTCGGTGGCTCTGCGTCGCATGCTTACACATGCTCGAAGCATGCAGTGGTGGGGTTGACAAAAAATGCGGCGGTAGAATTAGGGCAGTTTGGGATTAGGGTGAATTGCTTGTCGCCTTATGCATTGGCGACACCGTTGGCAACGAGGTTTGTGGGGTTGGAGGATGAGGAGTTTGAGATGGCTATAAACTCTATGGCGAATCTAAAAGGTGTCACGCTTAAGGCTGAGGATGTAGCCAATGCTGCTTTATTTTTTGCCAGTGATGAAAGTAGGTATGTGAGTGGCCACAATTTGTTGATTGATGGAGGCTTTAGTATTGTTAATCCTAATATAAAAAATTTTCAATACCCTCAACATTAG

Protein sequence

MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANAALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH

Homology

[Close]

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.0e-95
Identity = 181/277 (65.34%), Postives = 217/277 (78.34%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           M +T  + T    RRLEGKVALITGGASGIGE TAKLF  HGAKV IAD+QD+LGH++  
Sbjct: 1   MAATSQVLTAIA-RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVE 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            I G +NS YIHCDVT+E  V+ AV   V T+GKLDIM +NAGI DP++PRIIDNEK DF
Sbjct: 61  AI-GTSNSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           +RV S+N+TGVFL +KHAA+ MIP ++G+IISTAS++S +GG +SHAY  SKHAV+GLT+
Sbjct: 121 ERVFSVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTR 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLE-DEEFEMAINSMANLKGVTLKAEDVAN 240
           N AVELGQFGIRVNCLSP+ L T L  +F G++ +EEFE  IN   NLKG     EDVAN
Sbjct: 181 NLAVELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVAN 240

Query: 241 AALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYP 277
           AAL+ ASDE++YVSGHNL IDGGFS+ N  IK FQYP
Sbjct: 241 AALYLASDEAKYVSGHNLFIDGGFSVCNSVIKVFQYP 275

[Close]

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 8.3e-82
Identity = 159/262 (60.69%), Postives = 198/262 (75.57%), Query Frame = 0


Query: 14  RRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTNSLYIHC 73
           R+L GKVA+ITGGASGIG CTA+LFV HGA+VV+ADIQD+LG +L A  LGP  S Y+HC
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAE-LGPDASSYVHC 72

Query: 74  DVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSINITGVFL 133
           DVT+E  V AAV  AV  FGKLD+M NNAG+  P   R+ +  K DF+RVL++N+ G FL
Sbjct: 73  DVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFL 132

Query: 134 GIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 193
           G KHAA+ M P + GSIISTAS++S V G+ASHAYT SKHA+VG T+NAA ELG+ GIRV
Sbjct: 133 GTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRV 192

Query: 194 NCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGV-TLKAEDVANAALFFASDESRYV 253
           NC+SP  +ATPLA   +G++DE  E  + + ANLKG   LKA+D+A AALF ASD+ RYV
Sbjct: 193 NCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYV 252

Query: 254 SGHNLLIDGGFSIVNPNIKNFQ 275
           SG NL +DGG S+VN +   F+
Sbjct: 253 SGQNLRVDGGLSVVNSSFGFFR 273

[Close]

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.3e-70
Identity = 139/268 (51.87%), Postives = 177/268 (66.04%), Query Frame = 0


Query: 2   TSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCAT 61
           TST   ST     RL+ KVA+ITGGA GIGE TAKLFV +GAKVVIADI DD G  +C  
Sbjct: 4   TSTPASST----NRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNN 63

Query: 62  ILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFD 121
           I  P    ++HCDVT +  V+  V   +   GKLDIM  N G++  +   I++    DF 
Sbjct: 64  IGSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFK 123

Query: 122 RVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGG-SASHAYTCSKHAVVGLTK 181
           RV+ IN+ G FL  KHAA+ MIP K GSI+ TAS++S+  G   SH YT +KHAV+GLT 
Sbjct: 124 RVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTT 183

Query: 182 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 241
           +   ELGQ GIRVNC+SPY +A+PL T   G++    E   +  ANLKG+ L+AEDVA+A
Sbjct: 184 SLCTELGQHGIRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADA 243

Query: 242 ALFFASDESRYVSGHNLLIDGGFSIVNP 269
             + A DES+YVSG NL+IDGG++  NP
Sbjct: 244 VAYLAGDESKYVSGLNLVIDGGYTRTNP 267

[Close]

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.3e-70
Identity = 138/266 (51.88%), Postives = 182/266 (68.42%), Query Frame = 0


Query: 15  RLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTNSLYIHCD 74
           RLEGKVAL+TGGASGIGE  A+LF+ HGAK+ I D+QD+LG  +   + G  ++ Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 75  VTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSINITGVFLG 134
           VT E  V+ AV    E +G +DIM+NNAGI       I D + ++F +V  IN+ GVFLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 135 IKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 194
           +KHAA+ MIP   GSI+S ASV+S + G+  H YT +KHAVVGLTK+ A ELG+ GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 195 CLSPYALATPLATRFVGLEDEE------FEMAINSMANLKGVTLKAEDVANAALFFASDE 254
           C+SPYA+ T L+  ++   + +      F   + S ANLKGV L   DVA A L+ A++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 255 SRYVSGHNLLIDGGFSIVNPNIKNFQ 275
           S+YVSG NL+IDGGFSI N  ++ F+
Sbjct: 242 SKYVSGLNLVIDGGFSIANHTLQVFE 267

[Close]

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.7e-69
Identity = 137/269 (50.93%), Postives = 178/269 (66.17%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           M ST    ++T   RL+ KVA+ITGGA GIGE TAKLFV +GAKVVIADI DD G  +C 
Sbjct: 1   MGSTSTPDSST--NRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCN 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            I  P    ++HCDVT +  V+  V   +   GKLDIM  N G++  +   I++    DF
Sbjct: 61  NIGSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGG-SASHAYTCSKHAVVGLT 180
            RV+ IN+ G FL  KHAA+ MIP K GSI+ TAS++S+  G   SH YT +KHAV+GLT
Sbjct: 121 KRVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLT 180

Query: 181 KNAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVAN 240
            +   ELG++GIRVNC+SPY +A+PL T   G++    E   +  ANLKG  L+AEDVA+
Sbjct: 181 TSLCTELGEYGIRVNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVAD 240

Query: 241 AALFFASDESRYVSGHNLLIDGGFSIVNP 269
           A  + A DES+YVSG NL+IDGG++  NP
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTNP 267

[Close]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL
Match: A0A0A0LGR0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 3.9e-135
Identity = 246/277 (88.81%), Postives = 259/277 (93.50%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVISTTTG RRLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCA
Sbjct: 1   MTSSEVISTTTGQRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLDIMMNNAGI DPSKPRIIDN+KHDF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLSIN+TGVFLGIKHAAQAMIPVKTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSINVTGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPY LATPLAT FV L+  EFE   +S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQ 278
           ALF ASDESRYVSGHNL +DGGFSIVNPNI+ FQYPQ
Sbjct: 241 ALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYPQ 277

[Close]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL
Match: A0A5A7TKJ5 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004260 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.3e-133
Identity = 243/274 (88.69%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVIS TTG RRLEGKVALITGGASGIGECTAK+FVHHGAKVVIADIQDDLGHALCA
Sbjct: 1   MTSSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLD+MMNNAGIVDPSKPRIIDN++ DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVL+IN+TGVFLGIKHAAQAMIP KTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLAT+FVGL   EFE  + S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQ 275
           ALF ASDESRYVSGHNLLIDGGFSIVNPNI  FQ
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

[Close]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL
Match: A0A1S3B7H5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.3e-133
Identity = 243/274 (88.69%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVIS TTG RRLEGKVALITGGASGIGECTAK+FVHHGAKVVIADIQDDLGHALCA
Sbjct: 1   MTSSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLD+MMNNAGIVDPSKPRIIDN++ DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVL+IN+TGVFLGIKHAAQAMIP KTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLAT+FVGL   EFE  + S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQ 275
           ALF ASDESRYVSGHNLLIDGGFSIVNPNI  FQ
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

[Close]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL
Match: A0A6J1J123 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481719 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.8e-130
Identity = 237/277 (85.56%), Postives = 257/277 (92.78%), Query Frame = 0


Query: 3   STEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATI 62
           ++++IS  T PRRLEGKVA+ITGGASGIGECTAKLFVHHGAKVVIADIQDDLG ALCAT 
Sbjct: 2   ASQLISAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATA 61

Query: 63  L-GPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFD 122
           L GPTN LY+HCDVTDE+Q++AAV AAVE FGKLDIMMNNAGI DPSKPRIIDN K DF+
Sbjct: 62  LGGPTNCLYVHCDVTDEAQIKAAVEAAVEAFGKLDIMMNNAGIADPSKPRIIDNHKEDFE 121

Query: 123 RVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTKN 182
           RVL+IN+TGVFLGIKHAAQAMIPVKTGSIISTAS+ASYVGGSASHAYTCSKHAVVGLTKN
Sbjct: 122 RVLNINVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKN 181

Query: 183 AAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANAA 242
           AAVELGQFGIRVNCLSP+A+ TPLATRFVGLE EEFE A+NSMANLKGVTL+ EDVANAA
Sbjct: 182 AAVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAA 241

Query: 243 LFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH 279
           LF ASDESRYVSGHNLLIDGGF I NPNIK F+YP++
Sbjct: 242 LFLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPEN 278

[Close]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL
Match: A0A0A0LEP1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778280 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 5.0e-130
Identity = 240/278 (86.33%), Postives = 252/278 (90.65%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           M S E+ISTTT  RRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA
Sbjct: 1   MASNEIISTTTWQRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVT+ESQ      AAVETFGKLDIMMNNAGI DPSKPRIIDN+KHDF
Sbjct: 61  NVLGSTNSLYVHCDVTEESQ------AAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLSIN+TGVFLGIKHAAQAMIP KTGSI+STASVASY GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSP+AL TPLAT+FVGL+  EFE  + S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH 279
           ALF ASDESRYVSGHNL IDGGFSIVNPNI  FQYPQ+
Sbjct: 241 ALFLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQN 272

[Close]

BLAST of Lsi03G018580 vs. NCBI nr
Match: XP_038903548.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 508.4 bits (1308), Expect = 3.8e-140
Identity = 254/278 (91.37%), Postives = 266/278 (95.68%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVIS  TGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIAD+QDDLGHALC 
Sbjct: 1   MTSSEVISAATGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADVQDDLGHALCN 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
           TILGPTNSLY+HCDVTDESQVQAAVVAAV+TFGKLDIMMNNAGI DPSKPRIIDNEKHDF
Sbjct: 61  TILGPTNSLYVHCDVTDESQVQAAVVAAVKTFGKLDIMMNNAGIADPSKPRIIDNEKHDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLS+N+TGVFLGIKHAAQAMIP KTGSI+STASVASYVGGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSVNVTGVFLGIKHAAQAMIPAKTGSILSTASVASYVGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLAT+F  LE EEFEMA+NSMANLKGVTLKA+DVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFTRLEGEEFEMAMNSMANLKGVTLKAQDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH 279
           ALF ASDESRYVSGHNLLIDGGF IVNPNIK FQYP++
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFGIVNPNIKIFQYPEN 278

[Close]

BLAST of Lsi03G018580 vs. NCBI nr
Match: XP_004136700.3 (secoisolariciresinol dehydrogenase [Cucumis sativus])

HSP 1 Score: 487.6 bits (1254), Expect = 6.9e-134
Identity = 245/277 (88.45%), Postives = 258/277 (93.14%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVISTTTG RRLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCA
Sbjct: 1   MTSSEVISTTTGQRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLDIMMNNAGI DPSKPRIIDN+K DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLS+NITGVFLGIKHAAQAMIPVKTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPY LATPLAT FV L+  EFE   +S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQ 278
           ALF ASDESRYVSGHNL +DGGFSIVNPNI+ FQYPQ
Sbjct: 241 ALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYPQ 277

[Close]

BLAST of Lsi03G018580 vs. NCBI nr
Match: KAE8651098.1 (hypothetical protein Csa_002484 [Cucumis sativus])

HSP 1 Score: 487.6 bits (1254), Expect = 6.9e-134
Identity = 246/278 (88.49%), Postives = 258/278 (92.81%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVISTTTG RRLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCA
Sbjct: 1   MTSSEVISTTTGQRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLDIMMNNAGI DPSKPRIIDN+K DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLS+NITGVFLGIKHAAQAMIPVKTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPY LATPLAT FV L+  EFE   +S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH 279
           ALF ASDESRYVSGHNL IDGGFSIVNPNI  FQYPQ+
Sbjct: 241 ALFLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQN 278

[Close]

BLAST of Lsi03G018580 vs. NCBI nr
Match: XP_004136701.3 (secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus])

HSP 1 Score: 485.7 bits (1249), Expect = 2.6e-133
Identity = 244/278 (87.77%), Postives = 256/278 (92.09%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           M S E+ISTTT  RRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA
Sbjct: 1   MASNEIISTTTWQRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVT+ESQVQ AV AAVETFGKLDIMMNNAGI DPSKPRIIDN+KHDF
Sbjct: 61  NVLGSTNSLYVHCDVTEESQVQEAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVLSIN+TGVFLGIKHAAQAMIP KTGSI+STASVASY GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSP+AL TPLAT+FVGL+  EFE  + S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQYPQH 279
           ALF ASDESRYVSGHNL IDGGFSIVNPNI  FQYPQ+
Sbjct: 241 ALFLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQN 278

[Close]

BLAST of Lsi03G018580 vs. NCBI nr
Match: XP_008442883.1 (PREDICTED: secoisolariciresinol dehydrogenase-like [Cucumis melo] >KAA0043844.1 secoisolariciresinol dehydrogenase-like [Cucumis melo var. makuwa] >TYK25290.1 secoisolariciresinol dehydrogenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 243/274 (88.69%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MTSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60
           MTS+EVIS TTG RRLEGKVALITGGASGIGECTAK+FVHHGAKVVIADIQDDLGHALCA
Sbjct: 1   MTSSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TILGPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDF 120
            +LG TNSLY+HCDVTDESQVQAAV AAVETFGKLD+MMNNAGIVDPSKPRIIDN++ DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDF 120

Query: 121 DRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTK 180
           DRVL+IN+TGVFLGIKHAAQAMIP KTGSI+STASVASY+GGSASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLAT+FVGL   EFE  + S ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQ 275
           ALF ASDESRYVSGHNLLIDGGFSIVNPNI  FQ
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

[Close]

BLAST of Lsi03G018580 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.7e-67
Identity = 142/283 (50.18%), Postives = 183/283 (64.66%), Query Frame = 0


Query: 2   TSTEVISTTTGP-RRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 61
           T+TE  S ++ P +RL GKVALITGGA+GIGE   +LF  HGAKV I D+QDDLG  +C 
Sbjct: 3   TNTESSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCK 62

Query: 62  TIL---GPTNSLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEK 121
           ++L       + +IH DV  E  +  AV  AV+ FG LDI++NNAG+     P I +   
Sbjct: 63  SLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSL 122

Query: 122 HDFDRVLSINITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVG 181
            +F+    +N+ G FL +KHAA+ MIP K GSI+S  SV   VGG   H+Y  SKHAV+G
Sbjct: 123 SEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLG 182

Query: 182 LTKNAAVELGQFGIRVNCLSPYALATPLATRFVGLEDEE------FEMAINSMANLKGVT 241
           LT++ A ELGQ GIRVNC+SPYA+AT LA   +  E+        F     + ANLKGV 
Sbjct: 183 LTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVE 242

Query: 242 LKAEDVANAALFFASDESRYVSGHNLLIDGGFSIVNPNIKNFQ 275
           L  +DVANA LF ASD+SRY+SG NL+IDGGF+  N + K F+
Sbjct: 243 LTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

[Close]

BLAST of Lsi03G018580 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 4.5e-67
Identity = 141/276 (51.09%), Postives = 185/276 (67.03%), Query Frame = 0


Query: 2   TSTEVISTTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCAT 61
           T  E  +TT  P+RLEGKVA+ITGGA GIG+ T  LF  HGA VVIAD+ +  G +L  +
Sbjct: 18  TIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKS 77

Query: 62  ILGPTNS---LYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPR--IIDNE 121
           +     S    +I CDV+ E+ V+  V   V  +G+LDI+ NNAG++   K    I+D +
Sbjct: 78  LSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFD 137

Query: 122 KHDFDRVLSINITGVFLGIKHAAQAMIPVK-TGSIISTASVASYVGGSASHAYTCSKHAV 181
             +FD V+ +N+ GV LG+KH A+AMI     G IISTASVA  +GG   HAYT SKHA+
Sbjct: 138 ADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAI 197

Query: 182 VGLTKNAAVELGQFGIRVNCLSPYALATPL------ATRFVGLED---EEFEMAINSMAN 241
           VGLTKNAA ELG++GIRVNC+SP+ +AT +       T    +ED   EE E  + S+AN
Sbjct: 198 VGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLAN 257

Query: 242 LKGVTLKAEDVANAALFFASDESRYVSGHNLLIDGG 263
           LKG TL+A D+A AAL+ ASDES+YV+GHNL++DGG
Sbjct: 258 LKGETLRANDIAEAALYLASDESKYVNGHNLVVDGG 293

[Close]

BLAST of Lsi03G018580 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 4.3e-65
Identity = 126/256 (49.22%), Postives = 178/256 (69.53%), Query Frame = 0


Query: 14  RRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTNSLYIHC 73
           +RL+GK+ +ITGGASGIG  + +LF  HGA+VVI D+QD+LG  +  +I G   + Y HC
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSI-GEDKASYYHC 63

Query: 74  DVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSINITGVFL 133
           DVT+E++V+ AV   VE +GKLD++ +NAG+++P    I+D   ++ DR ++IN+ G   
Sbjct: 64  DVTNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAA 123

Query: 134 GIKHAAQAMIPVK-TGSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 193
            IKHAA+AM+     GSI+ T SVA+ + G+A H YT SKH ++GL K+A+  LG++GIR
Sbjct: 124 FIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIR 183

Query: 194 VNCLSPYALATPLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANAALFFASDESRYV 253
           VN ++P+ +ATPL      +E    E   ++ ANLKG+ LKA  VA AALF ASDES YV
Sbjct: 184 VNGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYV 243

Query: 254 SGHNLLIDGGFSIVNP 269
           SG NL +DGG+S+V P
Sbjct: 244 SGQNLAVDGGYSVVKP 257

[Close]

BLAST of Lsi03G018580 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-61
Identity = 128/264 (48.48%), Postives = 171/264 (64.77%), Query Frame = 0


Query: 8   STTTGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTN 67
           S+ +  R+LEGKVALITGGASGIG+ TA  F+ HGAKV+IADIQ  +G       LGP +
Sbjct: 70  SSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRE-TEQELGP-S 129

Query: 68  SLYIHCDVTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSIN 127
             Y  CDVT ES +  AV  AV    KLDIM NNAGI   + P I+D + + FD+V++ N
Sbjct: 130 CAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTN 189

Query: 128 ITGVFLGIKHAAQAMIPVKTGSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELG 187
           + GV  GIKHAA+ MIP  +GSII   SV   +GG A H Y+ SK AV+G+ ++ A EL 
Sbjct: 190 VRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELC 249

Query: 188 QFGIRVNCLSPYALAT-----PLATRFVGLEDEEFEMAINSMANLKGVTLKAEDVANAAL 247
           +  IRVNC+SP+A+ T      +   + G++D      + S   L G   +  DVANAA+
Sbjct: 250 KHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAV 309

Query: 248 FFASDESRYVSGHNLLIDGGFSIV 267
           + ASD+S+YV+GHNL++DGGF+ V
Sbjct: 310 YLASDDSKYVNGHNLVVDGGFTTV 331

[Close]

BLAST of Lsi03G018580 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-60
Identity = 126/259 (48.65%), Postives = 172/259 (66.41%), Query Frame = 0


Query: 15  RLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCATILGPTNSLYIHCD 74
           RL+GK+A+ITGGASGIG    +LF  HGAKVVI D Q++LG  +  ++ G   + +  CD
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSV-GKDKASFYRCD 64

Query: 75  VTDESQVQAAVVAAVETFGKLDIMMNNAGIVDPSKPRIIDNEKHDFDRVLSINITGVFLG 134
           VT+E +V+ AV   VE +GKLD++ +NAG+++      +D     FDR +++N+ G    
Sbjct: 65  VTNEKEVENAVKFTVEKYGKLDVLFSNAGVME-QPGSFLDLNLEQFDRTMAVNVRGAAAF 124

Query: 135 IKHAAQAMIPVKT-GSIISTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 194
           IKHAA+AM+   T GSI+ T SVAS +GG   HAYT SKHA++GL K+A   LG++GIRV
Sbjct: 125 IKHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRV 184

Query: 195 NCLSPYALATPLATRFVGLEDEEFEMAINSMAN----LKGVTLKAEDVANAALFFASDES 254
           N ++PYA+AT + +R     DEE    +   +     LKGV LKA  VA AALF ASD+S
Sbjct: 185 NGVAPYAVATAINSR-----DEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDS 244

Query: 255 RYVSGHNLLIDGGFSIVNP 269
            YVSG NL +DGG+S+V P
Sbjct: 245 AYVSGQNLAVDGGYSVVKP 256

The following BLAST results are available for this feature:

BLAST of Lsi03G018580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	5.0e-95	65.34	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	8.3e-82	60.69	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
A3F5F0	7.3e-70	51.87	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
F1SWA0	7.3e-70	51.88	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
Q94KL8	4.7e-69	50.93	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]

BLAST of Lsi03G018580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGR0	3.9e-135	88.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1	[more]
A0A5A7TKJ5	6.3e-133	88.69	Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3B7H5	6.3e-133	88.69	secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 ...	[more]
A0A6J1J123	3.8e-130	85.56	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
A0A0A0LEP1	5.0e-130	86.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778280 PE=4 SV=1	[more]

BLAST of Lsi03G018580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903548.1	3.8e-140	91.37	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
XP_004136700.3	6.9e-134	88.45	secoisolariciresinol dehydrogenase [Cucumis sativus]	[more]
KAE8651098.1	6.9e-134	88.49	hypothetical protein Csa_002484 [Cucumis sativus]	[more]
XP_004136701.3	2.6e-133	87.77	secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus]	[more]
XP_008442883.1	1.3e-132	88.69	PREDICTED: secoisolariciresinol dehydrogenase-like [Cucumis melo] >KAA0043844.1 ...	[more]

BLAST of Lsi03G018580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52340.1	2.7e-67	50.18	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	4.5e-67	51.09	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	4.3e-65	49.22	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	4.9e-61	48.48	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	1.1e-60	48.65	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 227..247 score: 31.21 coord: 168..187 score: 42.87 coord: 142..158 score: 32.97 coord: 20..37 score: 49.72 coord: 93..104 score: 57.66 coord: 189..206 score: 37.48
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 148..156 score: 46.12 coord: 168..187 score: 37.39 coord: 93..104 score: 50.92
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 25..264 e-value: 3.1E-67 score: 226.5
None	No IPR available	GENE3D	3.40.50.720		coord: 6..274 e-value: 6.3E-81 score: 273.4
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 16..267 e-value: 5.2E-21 score: 72.8
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 22..212 e-value: 2.5E-17 score: 60.8
None	No IPR available	PANTHER	PTHR43180:SF50	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 13..275
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 13..275
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 15..264 e-value: 1.82056E-118 score: 337.891
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 155..183
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 17..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G018580.1	Lsi03G018580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity