Lsi03G018430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G018430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr03: 29870684 .. 29872051 (-)
RNA-Seq Expression	Lsi03G018430
Synteny	Lsi03G018430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCTCCTTCCCATCTCTGTTTCTTCTACATTCTCTTCTTCTCCTCTCTTTCCGCCATTGCCAACACCAACCCCATCACCCTCCCTCTCAACGCCTTCCCCCACCTTCCTTCTTCAGATCCACTCCAAACTCTCACTTTTCTCACCTCAACTTCTCAAACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCTCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTTGGTACCCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGTACTTCCAGATATCTCTGTTCCGAATGTTCCTTCCCCAAAATAGATCCCGCCGGAATCCCCAGATTTGTCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCTTGGATTTTTGGCCCTGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTGGTTCAATACGGTTCCGGCTCCACGGCCGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCGATAAGAAAATCCCCAATTTCGTTGTTGGGTGCTCGTTTTTGTCGATCCATCAACCCTCCGGAATCGCCGGATTCGGTCGGGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCCTACTGCCTTGCGTCTCGGAAATTTGACGACTCGCCACATTCCGGCCAGCTGATTCTAGATTCCACCGGCGCGAAAACCAGCGGCCTCAGCTACACGCCGTTCCGGCAGAACCCCTCTGTTTCTAACCACGCTTATAAAGAATACTATTACTTAACCATACGCAAAATCCTCGTCGGGAAGCAGGCTGTGAATGTGCCGTACAAGTTTCTGGTACCGGGCCCCGACGGCAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTCTTCGAGGCAGTGGCGCAAGAGTTCGAGAAGCAGTTGGCGAATCGGACGAGAGCCACCGATGTGGAATCTCTCACCGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCGGTGGAGTTTCCGGAGTTGATTTTGCAGTTTAAAGGCGGAGCGAAATGGGCTCTGCCATTGAGTAACTATTTCGCTTTAGTCAGTAGCTCTGGCGTGGCGTGTTTGACGGTCGTGACGCATAAGACTGAGGCGGGCGGCGGCGGTGGGCCGTCTGTGATTTTGGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAGTATGATTTGGTGAATGAAAGATTGGGATTTCGGCAAAAGACTTGCACTTAG

mRNA sequence

ATGGCGTCTCCTTCCCATCTCTGTTTCTTCTACATTCTCTTCTTCTCCTCTCTTTCCGCCATTGCCAACACCAACCCCATCACCCTCCCTCTCAACGCCTTCCCCCACCTTCCTTCTTCAGATCCACTCCAAACTCTCACTTTTCTCACCTCAACTTCTCAAACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCTCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTTGGTACCCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGTACTTCCAGATATCTCTGTTCCGAATGTTCCTTCCCCAAAATAGATCCCGCCGGAATCCCCAGATTTGTCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCTTGGATTTTTGGCCCTGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTGGTTCAATACGGTTCCGGCTCCACGGCCGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCGATAAGAAAATCCCCAATTTCGTTGTTGGGTGCTCGTTTTTGTCGATCCATCAACCCTCCGGAATCGCCGGATTCGGTCGGGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCCTACTGCCTTGCGTCTCGGAAATTTGACGACTCGCCACATTCCGGCCAGCTGATTCTAGATTCCACCGGCGCGAAAACCAGCGGCCTCAGCTACACGCCGTTCCGGCAGAACCCCTCTGTTTCTAACCACGCTTATAAAGAATACTATTACTTAACCATACGCAAAATCCTCGTCGGGAAGCAGGCTGTGAATGTGCCGTACAAGTTTCTGGTACCGGGCCCCGACGGCAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTCTTCGAGGCAGTGGCGCAAGAGTTCGAGAAGCAGTTGGCGAATCGGACGAGAGCCACCGATGTGGAATCTCTCACCGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCGGTGGAGTTTCCGGAGTTGATTTTGCAGTTTAAAGGCGGAGCGAAATGGGCTCTGCCATTGAGTAACTATTTCGCTTTAGTCAGTAGCTCTGGCGTGGCGTGTTTGACGGTCGTGACGCATAAGACTGAGGCGGGCGGCGGCGGTGGGCCGTCTGTGATTTTGGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAGTATGATTTGGTGAATGAAAGATTGGGATTTCGGCAAAAGACTTGCACTTAG

Coding sequence (CDS)

ATGGCGTCTCCTTCCCATCTCTGTTTCTTCTACATTCTCTTCTTCTCCTCTCTTTCCGCCATTGCCAACACCAACCCCATCACCCTCCCTCTCAACGCCTTCCCCCACCTTCCTTCTTCAGATCCACTCCAAACTCTCACTTTTCTCACCTCAACTTCTCAAACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCTCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTTGGTACCCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGTACTTCCAGATATCTCTGTTCCGAATGTTCCTTCCCCAAAATAGATCCCGCCGGAATCCCCAGATTTGTCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCTTGGATTTTTGGCCCTGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTGGTTCAATACGGTTCCGGCTCCACGGCCGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCGATAAGAAAATCCCCAATTTCGTTGTTGGGTGCTCGTTTTTGTCGATCCATCAACCCTCCGGAATCGCCGGATTCGGTCGGGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCCTACTGCCTTGCGTCTCGGAAATTTGACGACTCGCCACATTCCGGCCAGCTGATTCTAGATTCCACCGGCGCGAAAACCAGCGGCCTCAGCTACACGCCGTTCCGGCAGAACCCCTCTGTTTCTAACCACGCTTATAAAGAATACTATTACTTAACCATACGCAAAATCCTCGTCGGGAAGCAGGCTGTGAATGTGCCGTACAAGTTTCTGGTACCGGGCCCCGACGGCAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTCTTCGAGGCAGTGGCGCAAGAGTTCGAGAAGCAGTTGGCGAATCGGACGAGAGCCACCGATGTGGAATCTCTCACCGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCGGTGGAGTTTCCGGAGTTGATTTTGCAGTTTAAAGGCGGAGCGAAATGGGCTCTGCCATTGAGTAACTATTTCGCTTTAGTCAGTAGCTCTGGCGTGGCGTGTTTGACGGTCGTGACGCATAAGACTGAGGCGGGCGGCGGCGGTGGGCCGTCTGTGATTTTGGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAGTATGATTTGGTGAATGAAAGATTGGGATTTCGGCAAAAGACTTGCACTTAG

Protein sequence

MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQIKTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT

Homology

[Close]

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.2e-49
Identity = 146/488 (29.92%), Postives = 221/488 (45.29%), Query Frame = 0


Query: 14  FFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQIKTPKSNSVSKSPL 73
           F  S+S+++    + L  +      SS PL  L   +S S  R  +    +       P+
Sbjct: 18  FHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 77

Query: 74  SPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVP 133
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P+       
Sbjct: 78  S--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSP----PS 137

Query: 134 KLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTENCTQT---CPAYVVQYGS 193
            LSSS+  V C +P C+        S  C   N      +T +C  +   CP +   YG 
Sbjct: 138 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 197

Query: 194 GSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 253
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 198 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 257

Query: 254 KFAYCLASRKFDDS--PHSGQLIL--------------------DSTGAKTSGLSYTPFR 313
            F+YCL S  FD         LIL                    D    K +   +T   
Sbjct: 258 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 317

Query: 314 QNPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPV 373
           +NP    H Y  +Y ++++ I +GK+ +  P        +G GG ++DSG+TFT +    
Sbjct: 318 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 377

Query: 374 FEAVAQEFEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEFPELILQFKGG-AKWALPL 433
           + +V +EF+ ++     RA  VE  +G+ PC+ +  +++V+ P L+L F G  +   LP 
Sbjct: 378 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKVPALVLHFAGNRSSVTLPR 437

Query: 434 SNYFALVSSSG--------VACLTVVTHKTEAGGGGGPSVILGAFQQQNFYVEYDLVNER 455
            NYF      G        + CL ++    E+   GG   ILG +QQQ F V YDL+N R
Sbjct: 438 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 491

[Close]

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.1e-38
Identity = 142/464 (30.60%), Postives = 202/464 (43.53%), Query Frame = 0


Query: 15  FSSLSAIANTNPITLPLNAFPHLPS--------SDPLQTLTFLTSTSQTRAHQI------ 74
           F S S   +++ ITL L+    L S        S  LQ  +    +  T A QI      
Sbjct: 60  FESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVT 119

Query: 75  KTPKSNSVSKSPLSPHSYGA--YSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 134
             P+    S S +S  S G+  Y T L  GTP + ++++ DTGS +VW  C     C  C
Sbjct: 120 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC 179

Query: 135 SFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 194
            + + DP     F P+ S +   + C +P C        +     CN + + C      Y
Sbjct: 180 -YSQSDPI----FDPRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTC-----LY 239

Query: 195 VVQYGSGS-TAGLLLSETLDFPDKKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQ 254
            V YG GS T G   +ETL F   ++    +GC   +       +G+ G G+G  S P Q
Sbjct: 240 QVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQ 299

Query: 255 MGLK---KFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYY 314
            G +   KF+YCL  R     P S   ++    A +    +TP   NP +       +YY
Sbjct: 300 TGHRFNQKFSYCLVDRSASSKPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYY 359

Query: 315 LTIRKILVGKQAV-NVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLAN 374
           + +  I VG   V  V          GNGG IIDSG++ T + +P + A+   F      
Sbjct: 360 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKT 419

Query: 375 RTRATDVESLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLT 434
             RA D         CFD+S    V+ P ++L F+ GA  +LP +NY   V ++G  C  
Sbjct: 420 LKRAPDFSLFD---TCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFA 479

Query: 435 VVTHKTEAGGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTC 455
                  AG  GG S+I G  QQQ F V YDL + R+GF    C
Sbjct: 480 F------AGTMGGLSII-GNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.8e-37
Identity = 119/383 (31.07%), Postives = 162/383 (42.30%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVPKLSSS 138
           G Y   LS GTP Q    I DTGS L+W  C     C   S P  +P G        SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQG--------SSS 152

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 198
              + C +  C  +  P               C+     Y   YG GS T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 199 FPDKKIPNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSP 258
           F    IPN   GC            +G+ G GRG  SLPSQ+ + KF+YC+       S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSST 272

Query: 259 HSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVGKQAVNV-PYKFLV 318
            S  L+     + T+G   T   Q+  +       +YY+T+  + VG   + + P  F +
Sbjct: 273 PSNLLLGSLANSVTAGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFAL 332

Query: 319 PGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESLTGLRPCFDISKD 378
              +G GG IIDSG+T T+     +++V QEF  Q+          S +G   CF    D
Sbjct: 333 NSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSD 392

Query: 379 KS-VEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGGGGGPSVILGAF 438
            S ++ P  ++ F GG    LP  NYF +  S+G+ CL +       G       I G  
Sbjct: 393 PSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICLAM-------GSSSQGMSIFGNI 434

Query: 439 QQQNFYVEYDLVNERLGFRQKTC 455
           QQQN  V YD  N  + F    C
Sbjct: 453 QQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-36
Identity = 127/386 (32.90%), Postives = 174/386 (45.08%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC-SFPKIDPAGIPRFVPKLSS 138
           G Y   ++ GTP  +   I DTGS L+W  C     C++C S P       P F P+ SS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCFSQP------TPIFNPQDSS 153

Query: 139 SSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTA-GLLLSETL 198
           S   + C++  C      D+ S         E C      Y   YG GST  G + +ET 
Sbjct: 154 SFSTLPCESQYC-----QDLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETF 213

Query: 199 DFPDKKIPNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDS 258
            F    +PN   GC            +G+ G G G  SLPSQ+G+ +F+YC+ S     S
Sbjct: 214 TFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSS 273

Query: 259 PHSGQLILDSTGAKTSGLSYTPFRQ--NPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKF 318
           P +  L   ++G      S T      NP+        YYY+T++ I VG   + +P   
Sbjct: 274 PSTLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSST 333

Query: 319 LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESLTGLRPCFDIS 378
                DG GG IIDSG+T T++ +  + AVAQ F  Q+      T  ES +GL  CF   
Sbjct: 334 FQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQP 393

Query: 379 KDKS-VEFPELILQFKGGAKWALPLSNYFALVS-SSGVACLTVVTHKTEAGGGGGPSVIL 438
            D S V+ PE+ +QF GG    L L     L+S + GV CL + +  ++ G       I 
Sbjct: 394 SDGSTVQVPEISMQFDGG---VLNLGEQNILISPAEGVICLAMGS-SSQLG-----ISIF 435

Query: 439 GAFQQQNFYVEYDLVNERLGFRQKTC 455
           G  QQQ   V YDL N  + F    C
Sbjct: 454 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.2e-30
Identity = 126/443 (28.44%), Postives = 185/443 (41.76%), Query Frame = 0


Query: 19  SAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQIKTPKSNSVSKSPLSPHSY 78
           S  A T+     ++   H+ SSD    LT+L+S    +      PK  SV  +  +    
Sbjct: 49  SPFAPTHVSASVIDTVLHMASSDS-HRLTYLSSLVAGK------PKPTSVPVASGNQLHI 108

Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVPKLSSS 138
           G Y      GTP Q + ++ DT +  VW PC+    CS CS           F    SS+
Sbjct: 109 GNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSG---CSGCSNASTS------FNTNSSST 168

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGST-AGLLLSETLD 198
              V C   +C    G      C S +P+   C     ++   YG  S+ +  L+ +TL 
Sbjct: 169 YSTVSCSTAQCTQARG----LTCPSSSPQPSVC-----SFNQSYGGDSSFSASLVQDTLT 228

Query: 199 FPDKKIPNFVVGC---SFLSIHQPSGIAGFGRGSESLPSQ---MGLKKFAYCLASRKFDD 258
                IPNF  GC   +  +   P G+ G GRG  SL SQ   +    F+YCL S  F  
Sbjct: 229 LAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTSLYSGVFSYCLPS--FRS 288

Query: 259 SPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKFL 318
              SG L L   G   S + YTP  +NP          YY+ +  + VG   V V   +L
Sbjct: 289 FYFSGSLKLGLLGQPKS-IRYTPLLRNP-----RRPSLYYVNLTGVSVGSVQVPVDPVYL 348

Query: 319 VPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESLTGLRPCFDISK 378
               +   G+IIDSG+  T   +PV+EA+  EF KQ+      +   +L     CF  S 
Sbjct: 349 TFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQV----NVSSFSTLGAFDTCF--SA 408

Query: 379 DKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGGGGGPSVILGAF 438
           D     P++ L         LP+ N     S+  + CL++   +  A        ++   
Sbjct: 409 DNENVAPKITLHMT-SLDLKLPMENTLIHSSAGTLTCLSMAGIRQNA---NAVLNVIANL 448

Query: 439 QQQNFYVEYDLVNERLGFRQKTC 455
           QQQN  + +D+ N R+G   + C
Sbjct: 469 QQQNLRILFDVPNSRIGIAPEPC 448

[Close]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL
Match: A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.9e-246
Identity = 416/455 (91.43%), Postives = 434/455 (95.38%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MASPS L FFYIL FSSLSAI+N+NPITLPLN+ PHL SSDPLQ LTFL S S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDS HSGQLILDS+G KTSGL+YT FRQNPSVSNHAYKEYYYL IRKI+VG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPV + VAQEFEKQLANRTRATDVE+L
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420
           TGLRPCFD+SK+KSVEFPELI QFKGGAKWALPL+NYFALVSSSGVACLTVVTH TE GG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGPSVILGAFQQQNFYVEYDLVNERLGFR++TCT
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL
Match: A0A1S3B6B5 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.9e-246
Identity = 416/455 (91.43%), Postives = 434/455 (95.38%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MASPS L FFYIL FSSLSAI+N+NPITLPLN+ PHL SSDPLQ LTFL S S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDS HSGQLILDS+G KTSGL+YT FRQNPSVSNHAYKEYYYL IRKI+VG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPV + VAQEFEKQLANRTRATDVE+L
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420
           TGLRPCFD+SK+KSVEFPELI QFKGGAKWALPL+NYFALVSSSGVACLTVVTH TE GG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGPSVILGAFQQQNFYVEYDLVNERLGFR++TCT
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL
Match: A0A0A0LBI9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778440 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.5e-245
Identity = 417/457 (91.25%), Postives = 436/457 (95.40%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MASPS L FFY+L FSSLSAIA++NPITLPLN+FPHL S DPLQ LTFL S+SQTRAHQI
Sbjct: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKC+WIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDSPHSGQLILDSTG K+SGL+YTPFRQNPSVSN+AYKEYYYL IRKI+VG
Sbjct: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYKFLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+EFEKQLAN TRATDVE+L
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTE--A 420
           TGLRPCFDISK+KSV+FPELI QFKGGAKWALPL+NYFALVSSSGVACLTVVTH+ E   
Sbjct: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGGGPSVILGAFQQQNFYVEYDLVN+RLGFRQ+TC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

[Close]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL
Match: A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.5e-240
Identity = 410/457 (89.72%), Postives = 429/457 (93.87%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MA P  LCFFYIL  SS+SAIA+TNPIT+PL++FPH  SSDPLQTL FL S SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTPK--SNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K PK  SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPAGIPRF+PKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDKK  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIL 300
           KFAYCLASRKFDDSPH+G+LILDS+GAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVE 360
           VGK+AV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQE EKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEA 420
           SLTGLRPCFDISKDKSVEFPEL  Q KGGAKW LPLSNYFALVSSSGVACLTVVTHKT A
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKT-A 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFRQ+TC+
Sbjct: 421 DSGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL
Match: A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 407/457 (89.06%), Postives = 426/457 (93.22%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MA P  LCFFYIL  SS+SAIA+TNPITLPL++FPH  SSDPLQTL FL S SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K P  KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPA IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFP+KKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIL 300
           KFAYCLASRKFDDSPH+G+LILDS+GAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVE 360
           VG +AV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQE EKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEA 420
           SLTGLRPCFDISKDKSVEFPEL    KGGAKWA PLSNYFALVSSSGVACLTVVTHK  A
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKA-A 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFRQ+TC+
Sbjct: 421 ESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Lsi03G018430 vs. NCBI nr
Match: XP_038905730.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 888.3 bits (2294), Expect = 2.9e-254
Identity = 433/455 (95.16%), Postives = 438/455 (96.26%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MA PS L FFYIL FSS+SAIANTNPITLPLNAFPHL SSDPLQTLTFL S SQ RAHQI
Sbjct: 1   MAPPSSLSFFYILLFSSVSAIANTNPITLPLNAFPHLSSSDPLQTLTFLASASQNRAHQI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVSKSPL PHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVSKSPLFPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKCAWIFGP+VKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPEVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDSPHSG+LILDSTG KTSGLSYTPFRQNPSVSNHAYKEYYYL IRKI VG
Sbjct: 241 AYCLASRKFDDSPHSGELILDSTGVKTSGLSYTPFRQNPSVSNHAYKEYYYLNIRKIFVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420
           TGLRPCFDISKDKSVEFPELI QFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG
Sbjct: 361 TGLRPCFDISKDKSVEFPELIFQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGPSVI GAFQQQNFYVEYDLVNE+LGFRQ+TCT
Sbjct: 421 GGGPSVIFGAFQQQNFYVEYDLVNEKLGFRQQTCT 455

[Close]

BLAST of Lsi03G018430 vs. NCBI nr
Match: XP_008442902.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043829.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK25303.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 416/455 (91.43%), Postives = 434/455 (95.38%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MASPS L FFYIL FSSLSAI+N+NPITLPLN+ PHL SSDPLQ LTFL S S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDS HSGQLILDS+G KTSGL+YT FRQNPSVSNHAYKEYYYL IRKI+VG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPV + VAQEFEKQLANRTRATDVE+L
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420
           TGLRPCFD+SK+KSVEFPELI QFKGGAKWALPL+NYFALVSSSGVACLTVVTH TE GG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGPSVILGAFQQQNFYVEYDLVNERLGFR++TCT
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of Lsi03G018430 vs. NCBI nr
Match: XP_004136706.1 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN59188.1 hypothetical protein Csa_002380 [Cucumis sativus])

HSP 1 Score: 856.7 bits (2212), Expect = 9.3e-245
Identity = 417/457 (91.25%), Postives = 436/457 (95.40%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MASPS L FFY+L FSSLSAIA++NPITLPLN+FPHL S DPLQ LTFL S+SQTRAHQI
Sbjct: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60

Query: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDP GIPRFVPKLSSSSKLVGCQNPKC+WIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 300
           AYCLASRKFDDSPHSGQLILDSTG K+SGL+YTPFRQNPSVSN+AYKEYYYL IRKI+VG
Sbjct: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300

Query: 301 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360
            QAV VPYKFLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+EFEKQLAN TRATDVE+L
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360

Query: 361 TGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTE--A 420
           TGLRPCFDISK+KSV+FPELI QFKGGAKWALPL+NYFALVSSSGVACLTVVTH+ E   
Sbjct: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           GGGGGPSVILGAFQQQNFYVEYDLVN+RLGFRQ+TC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

[Close]

BLAST of Lsi03G018430 vs. NCBI nr
Match: XP_022982947.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 840.9 bits (2171), Expect = 5.3e-240
Identity = 410/457 (89.72%), Postives = 429/457 (93.87%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MA P  LCFFYIL  SS+SAIA+TNPIT+PL++FPH  SSDPLQTL FL S SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTPK--SNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K PK  SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPAGIPRF+PKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDKK  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIL 300
           KFAYCLASRKFDDSPH+G+LILDS+GAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVE 360
           VGK+AV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQE EKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEA 420
           SLTGLRPCFDISKDKSVEFPEL  Q KGGAKW LPLSNYFALVSSSGVACLTVVTHKT A
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKT-A 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFRQ+TC+
Sbjct: 421 DSGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Lsi03G018430 vs. NCBI nr
Match: XP_023528159.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 836.3 bits (2159), Expect = 1.3e-238
Identity = 409/457 (89.50%), Postives = 428/457 (93.65%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQI 60
           MA P  LCFFYIL  SS+SAIA+TNPITLPL++FPH  SSDPLQTL FL S SQ RAHQI
Sbjct: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K P  KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPAGIPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDF +KKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFANKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIL 300
           KFAYCLASRKFDDSPH+G+LILDS+GAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVE 360
           VG +AV VPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQE EKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEA 420
           SLTGLRPCFDISKDKSVEFPEL  Q KGGAKWALPLSNYFALVSSSGVACLTVVTHK  A
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWALPLSNYFALVSSSGVACLTVVTHKA-A 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFRQ+TC+
Sbjct: 421 DSGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Lsi03G018430 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 6.4e-151
Identity = 266/474 (56.12%), Postives = 339/474 (71.52%), Query Frame = 0


Query: 5   SHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSS--DPLQTLTFLTSTSQTRAHQIK- 64
           S + FF+++F S +SA      + LPL+ F H   S  DP  +L  L  +S  RAH++K 
Sbjct: 3   SSIFFFFLIFLSVVSA------VKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKH 62

Query: 65  --------------TPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWF 124
                         T  S +V KSPLS  SYG YS  LSFGTP QT+  +FDTGSSLVW 
Sbjct: 63  GTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWL 122

Query: 125 PCTSRYLCSECSFPKIDPAGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPK 184
           PCTSRYLCS C F  +DP  IPRF+PK SSSSK++GCQ+PKC +++GP+V  QCR C+P 
Sbjct: 123 PCTSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPN 182

Query: 185 TENCTQTCPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRG 244
           T NCT  CP Y++QYG GSTAG+L++E LDFPD  +P+FVVGCS +S  QP+GIAGFGRG
Sbjct: 183 TRNCTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRG 242

Query: 245 SESLPSQMGLKKFAYCLASRKFDDSPHSGQLILD-----STGAKTSGLSYTPFRQNPSVS 304
             SLPSQM LK+F++CL SR+FDD+  +  L LD     ++G+KT GL+YTPFR+NP+VS
Sbjct: 243 PVSLPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVS 302

Query: 305 NHAYKEYYYLTIRKILVGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQ 364
           N A+ EYYYL +R+I VG++ V +PYK+L PG +G+GGSI+DSGSTFTFM++PVFE VA+
Sbjct: 303 NKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAE 362

Query: 365 EFEKQLANRTRATDVESLTGLRPCFDISKDKSVEFPELILQFKGGAKWALPLSNYFALVS 424
           EF  Q++N TR  D+E  TGL PCF+IS    V  PELI +FKGGAK  LPLSNYF  V 
Sbjct: 363 EFASQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVG 422

Query: 425 SSGVACLTVVTHKT-EAGGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTCT 456
           ++   CLTVV+ KT    GG GP++ILG+FQQQN+ VEYDL N+R GF +K C+
Sbjct: 423 NTDTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of Lsi03G018430 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.7e-50
Identity = 146/488 (29.92%), Postives = 221/488 (45.29%), Query Frame = 0


Query: 14  FFSSLSAIANTNPITLPLNAFPHLPSSDPLQTLTFLTSTSQTRAHQIKTPKSNSVSKSPL 73
           F  S+S+++    + L  +      SS PL  L   +S S  R  +    +       P+
Sbjct: 18  FHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 77

Query: 74  SPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVP 133
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P+       
Sbjct: 78  S--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSP----PS 137

Query: 134 KLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTENCTQT---CPAYVVQYGS 193
            LSSS+  V C +P C+        S  C   N      +T +C  +   CP +   YG 
Sbjct: 138 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 197

Query: 194 GSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 253
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 198 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 257

Query: 254 KFAYCLASRKFDDS--PHSGQLIL--------------------DSTGAKTSGLSYTPFR 313
            F+YCL S  FD         LIL                    D    K +   +T   
Sbjct: 258 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 317

Query: 314 QNPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPV 373
           +NP    H Y  +Y ++++ I +GK+ +  P        +G GG ++DSG+TFT +    
Sbjct: 318 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 377

Query: 374 FEAVAQEFEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEFPELILQFKGG-AKWALPL 433
           + +V +EF+ ++     RA  VE  +G+ PC+ +  +++V+ P L+L F G  +   LP 
Sbjct: 378 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKVPALVLHFAGNRSSVTLPR 437

Query: 434 SNYFALVSSSG--------VACLTVVTHKTEAGGGGGPSVILGAFQQQNFYVEYDLVNER 455
            NYF      G        + CL ++    E+   GG   ILG +QQQ F V YDL+N R
Sbjct: 438 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 491

[Close]

BLAST of Lsi03G018430 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-47
Identity = 147/488 (30.12%), Postives = 217/488 (44.47%), Query Frame = 0


Query: 1   MASPSHLCFFYILFFSSLSAIANTNPITLPLNAFPHLPSSDPL------QTLTFLTSTSQ 60
           M + +H+ F ++L    L+    TN      +  P   SS  L       +++  T  SQ
Sbjct: 1   METQTHVLFLFLLITLLLN---TTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQ 60

Query: 61  TRAHQIKTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTS-RY 120
           T+    K   S  V   PL     G Y   L+ GTP Q + +  DTGS L W PC +  +
Sbjct: 61  TQERIKKPLSSVDVVMEPLREVRDG-YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSF 120

Query: 121 LCSECSFPKIDPAGIPR-FVPKLSSSSKLVGCQNPKCAWI------FGPDVKSQCRSCNP 180
            C EC   K +    P  F P  SS+S    C +  C  I      F P   + C     
Sbjct: 121 DCIECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSML 180

Query: 181 KTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFG 240
               C + CP++   YG G   +G+L  + L    + +P F  GC   +  +P GIAGFG
Sbjct: 181 LKSTCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFG 240

Query: 241 RGSESLPSQMGL--KKFAYCLASRKFDDSPH-SGQLILDSTGAK---TSGLSYTPFRQNP 300
           RG  SLPSQ+G   K F++C    KF ++P+ S  LIL ++      T  L +TP    P
Sbjct: 241 RGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP 300

Query: 301 SVSNHAYKEYYYLTIRKILVGKQ--AVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVF 360
                 Y   YY+ +  I +G       VP         GNGG ++DSG+T+T + +P +
Sbjct: 301 -----MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFY 360

Query: 361 EAVAQEFEKQLANRTRATDVESLTGLRPCF----------DISKDKSVEFPELILQFKGG 420
             +    +  +    RAT+ ES TG   C+           +  D  + FP +   F   
Sbjct: 361 SQLLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNN 420

Query: 421 AKWALPLSN-YFALVSSSGVACLTVVTHKTEAGGGGGPSVILGAFQQQNFYVEYDLVNER 455
           A   LP  N ++A+ + S  + +  +  +    G  GP+ + G+FQQQN  V YDL  ER
Sbjct: 421 ATLLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKER 478

[Close]

BLAST of Lsi03G018430 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 167.5 bits (423), Expect = 2.4e-41
Identity = 126/397 (31.74%), Postives = 183/397 (46.10%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVPKLSSS 138
           G Y   +  GTP +   LI DTGS L W  C   Y C   +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 198
            K + C +P+C+ I  PD   QC S N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 199 F---------PDKKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 258
                      + K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 259 CLASRKFDDSPHSGQLIL--DSTGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKILVG 318
           CL  R   ++  S +LI   D      + L++T F        ++ + +YY+ I+ ILVG
Sbjct: 338 CLVDRN-SNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397

Query: 319 KQAVNVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEF-EKQLANRTRATDVES 378
            +A+++P +      DG+GG+IIDSG+T ++  +P +E +  +F EK   N     D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 379 LTGLRPCFDIS--KDKSVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTE 438
           L    PCF++S  ++ ++  PEL + F  G  W  P  N F  +S   + CL ++     
Sbjct: 458 LD---PCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCLAIL----- 517

Query: 439 AGGGGGPSVILGAFQQQNFYVEYDLVNERLGFRQKTC 455
            G       I+G +QQQNF++ YD    RLGF    C
Sbjct: 518 -GTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of Lsi03G018430 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-39
Identity = 116/381 (30.45%), Postives = 167/381 (43.83%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPAGIPRFVPKLSSS 138
           G Y T +  G P + ++++ DTGS + W  CT    C++C + + +P     F P  SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 198
            + + C  P+C  +      S+CR+          TC  Y V YG GS T G   +ETL 
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265

Query: 199 FPDKKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 258
                + N  VGC   +       +G+ G G G  +LPSQ+    F+YCL  R   DS  
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDR---DS-- 325

Query: 259 SGQLILDSTGAKTSGLSYTPFR-QNPSVSNHAYKEYYYLTIRKILVGKQAVNVPYKFLVP 318
                 DS      G S +P     P + NH    +YYL +  I VG + + +P      
Sbjct: 326 ------DSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 385

Query: 319 GPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESLTGLRPCFDISKDK 378
              G+GG IIDSG+  T +   ++ ++   F K   +  +A  V        C+++S   
Sbjct: 386 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFD---TCYNLSAKT 445

Query: 379 SVEFPELILQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGGGGGPSVILGAFQQ 438
           +VE P +   F GG   ALP  NY   V S G  CL      +          I+G  QQ
Sbjct: 446 TVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSL-------AIIGNVQQ 483

Query: 439 QNFYVEYDLVNERLGFRQKTC 455
           Q   V +DL N  +GF    C
Sbjct: 506 QGTRVTFDLANSLIGFSSNKC 483

The following BLAST results are available for this feature:

BLAST of Lsi03G018430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	5.2e-49	29.92	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	7.1e-38	30.60	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	7.8e-37	31.07	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.3e-36	32.90	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
O04496	3.2e-30	28.44	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

BLAST of Lsi03G018430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TRK2	6.9e-246	91.43	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B6B5	6.9e-246	91.43	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 S...	[more]
A0A0A0LBI9	4.5e-245	91.25	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G77844...	[more]
A0A6J1IXY3	2.5e-240	89.72	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640...	[more]
A0A6J1F3G5	1.5e-237	89.06	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114418...	[more]

BLAST of Lsi03G018430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905730.1	2.9e-254	95.16	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_008442902.1	1.4e-245	91.43	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043829.1 aspart...	[more]
XP_004136706.1	9.3e-245	91.25	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN59188.1 hypothetical ...	[more]
XP_022982947.1	5.3e-240	89.72	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
XP_023528159.1	1.3e-238	89.50	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G018430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	6.4e-151	56.12	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	3.7e-50	29.92	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	5.0e-47	30.12	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.4e-41	31.74	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.3e-39	30.45	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 87..107 score: 50.56 coord: 321..332 score: 29.09 coord: 426..441 score: 30.89
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 62..259 e-value: 3.7E-34 score: 120.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 260..455 e-value: 8.6E-51 score: 174.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 75..454
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 81..258 e-value: 1.7E-29 score: 103.3
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 290..450 e-value: 5.2E-35 score: 120.6
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 7..454
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 7..454
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 96..107
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 81..450 score: 33.56234
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 80..454 e-value: 1.20223E-79 score: 246.021

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G018430.1	Lsi03G018430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity