Lsi03G017140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G017140
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cytochrome P450, putative
Location	chr03: 28510341 .. 28514179 (-)
RNA-Seq Expression	Lsi03G017140
Synteny	Lsi03G017140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCTCCATCTTTGACAACACCACCTCTCCCTCTCAATTTTGGTGCTTCTGGTTCATCATTGCCCTCCTCCTCCATCTCCTCCTCAAAAAACATCTCTCAAACCCTAAACCCCCATCTCCTGGCACTCTCGCACCGGTCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCATCTCCTCACCCCAGTCCTCGTCACCTCATTCCAAACCCTAGCTTGTCGCTATGGTCCCCTCATCGAGCTCCGACTCGGCGCATCAAAGTGTGTCGTCGTCTCAACCGCTGCAGTCGCCAAAGACATCCTAAAAACCCACGAACACAACTTCCTCTCTCGACCGGAGTTCGGCGCCTCCGAGTACTTCATTTACCGAGGGTCGAGATTCGTCATGGCTCAATACGGACCCTATTGGCGATTCATGAAGAAGCTAACCATGACACGGCTTCTCTCGCCGCCGCAGTTGGCGGTCTCGACCGCCATCCGTAGCGACGAGATAGCAAAACTCGTGGAAAGAATAGAGGCAAATTCAAGGGAAAAGAAGCCCACAGATTTGAGATTGGAGTCTACTACTTTGACAAACAATATCATATCAAGGATGGTGTTTAGCACAAGGTGCTGTGGAGGGAAAGATGAGGCTCAAGAGATTAAGGATTTGGCTTGGAGGATCAATATGTTGGCTGGGAAGTTGAGTTTGGGTGATATTTTGGGACCTTTAAAAGTGTTTGATTTCTCCGGAAATGGAAAGAAATTTGTGAAAACTTTGAAGAAATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCTGCCAATAATGGTGGTGGGGATGCTCAAAGAAGTAGAGATTTGTTGGATATCTTGTTGGAGATTTACAAAGATCCAAATGCTGAGATGAAGATAACGAGAAACGATCTCAAGTCGTTCTTGCTTGTGAGTTTTTTAAATTTGTTTAGTTTTTATGTTTTTTTAGAAGAATTTTTAAAGTAGAGAAATAAGAAAACTATTTACATAAAATAGCAAAATTTTTAAATAATTGTGATCAGTCTAATAGAAGTTTATGAGAGTCTATTAGTGATAGATGATGATAGAAGTCTATCAATGTCCATCAGTATTTTTTTTTTTTTTTGTCTATTTTCAATAAATAGTTTGACATTTTTTTTATTTGTGAATTTTTTTTATTATTATTGTTTTTTCATTTTTTTAAATATTAGTATAGGTTTTTTTTTTATAATGAGAATTTAAGAGATAACCAGATATATATGTTGGGGAGAAAATTTTGTACGAAACAAGTAAAATAAGCTTTTTAAACTTATATTTCTTTTATAAGCTTAAAATATTATAAATAAATTTCCATCGGTTTATAAGCTTAAAATTTTCGAAAACCAAATAAATAAATTTACTATACACTTTTAAACTTATAAACATTTGGTTTTCGAAAATTTTAAGTTTATGAACACTATTTACCTTCATAAATTTTAATGTTATGTTATCAACTTTCTACTCATGTTTTCAACAACTAAGTCAAATTTTGAAAACTAAAAAAATATATTTTTTATTTTTAGAATTTGTCTAAAGATTCAAGTATTTTTTTCAAATAACGAAAATCATACTTGAAAATTTTAGAGGAAACAAATTTAAATTTTAAAAACTACAAACTAAAATCAAAATTGTAATCAAATAGGATCTTATCAAACCATTCTTTTCAAAAATAAAAAACAAAAATGAAATCTTTGTCTAATTTTTTTAACAAGAATATTTATCAAATATTATTTTAATAATCTATTAAAGAATAAAAGGATATTTTCAAAAAAAAAAAAAAATTGGCTTATGAAGTTTGAATTTCATTTCGATGTATAATGATCTTATTATAATCCTTGTTTATGATTTTGTTAGAACTTCATAATACTGTTATTAGGGGAAAAGGGGAGGACATTATTAGGTAAATTTCAGGACTCTTTTCAAATATAGAAAAATGAACCAAAATATTTACAAATATAGAAAAATTTTACAATCTATCACTAATAGATCACGATAGAATTCTATCGCTCTCTATCGATCAGGCGATAGAATTTATTTGTTTGAGCAATAGAAAGCGATAGAATTCTATCACGGTCTATCACTGAAATTTTTCTATATTTGTAAATAGTTTGATATTTTTTCTGTTTACAGTAATTTTCCTAAATTTCATGTAAGAAAAAATAATAATAAATTGTGAAGGGAAAAAAGCGAGTCTTAATAAACCTCCAAAAAAAATTTGATCCAAAAAATCTAAAATATATTGACAAAAAAAAAATTGGTGTTTGGTTGGTGATTTAAAAACAAATTTTGAAATTTGAAAAGGTTTTTACTATTTACCCTTTAAAACCACTGATGCTTAATTACTATCACTATAAAACAAGCTCGTATTTTATCTTTGTTATCTTTTCTTAATAACGAGAAACAATTTTTAAAAAGTAAGTCGACCGTTAGAATTATTAATTTTAGACAAAAGTATAAATAAGCTTACTCTTTAATTGAATTAGAATTCTGATTCAAACTCAACTGCCTATTGATCCGTTGTTTGAAAAATCGAAAATCTAGATGTGATTCTAAGAAAAAGAAAACAATGGAAGAAGAATAAATCACTTTTTTATTAAATATATTTGCGCATTTTTTACCATTTTACCATTTTATCATATTTTATTATATTAATTTCTACTCTACTTTCTCGGAGTTCATTTTCTAAAGAACTCCATTTTAAACATTTCGCTAGAAAAATATAGTTGTATTTTGTCATTTCTTCAAGGTGAGATAGTGTATGTTCAAGTAGCCTTATTTTCCTTGTTCACACTAGCTAAAATAATTTTCCTATTTTAAGGGTTAGTATTTGTACTACAAAATTTTCAACATCGAGTATCTATCACTTCTTTTATTACACTGATTACTTATATATTTATCTAAATTCAAGTATATAATTCAACAGATGCACACAAATTAATATTTCATAAGTCGAATGGTTAGATATCTATTTATGTAATTGTTGAGCTAAAAATAATACTGATAATTAATAAACGACTTAATAGATTGCAAACATGAACATAGTTTGACTGACATAAACTTGTATTCTCAATCTTGAGGTCTGAGATTCGATCTTCACCTTACACCTTGTCAAAAAAAAAAACCTCAATAGATAATCTCCCTCCCTTTTCTTTGGGTAGGACCTTTTTATGGCAGGCACTGACACAACGGCCACAGCTGTGCTATGGGCAATGGGAGAGCTTCTAAATCGTCCAGAATCGATCCAAAAGCTAAGAAAAGAGATAATATCAGTGGTTGGAAACAAGAAGCAGGTCCAAGAATCCGACCTCCCCAACCTCCCATATCTCCGAGCAGTGGTCAATGAAACACTCCGTCTCCACCCGTCGGCGCCAATCATCATCAGGGAATGTTTAGATGACTGTTACATCAATGGCTCACTAATCAAGGCCAAAACTCGAGTATTGGTCAATGCATATGCCGTCATGAGAGACCCAGAGTCTTGGTCAGAGCCTGACAGGTTCTTGCCAGAGAGGTTTTTGGAAGGCTCCCATGAGAATATCGGAGGCCATCGGATGGAGATGAAAGGGCAGAATTTTCGTTACATTCCGTTTGGGAGTGGAAAGAGAGGCTGTCCTGGATCTTCCCTTGCATTGTTAGTTTTTCCATGTGCAATTGCGACCATGGTGCAACGATTTGATTGGAAGACAGGTGGGAATGACAGTGGCAACATTGATTTGACACTTGGGTCGGGATTTGCTGCAGAGATGGCAACGCCACTTGTCTGTTATGCAAACTCCATTGATGGGAGTGATTAA

mRNA sequence

ATGACCTCCATCTTTGACAACACCACCTCTCCCTCTCAATTTTGGTGCTTCTGGTTCATCATTGCCCTCCTCCTCCATCTCCTCCTCAAAAAACATCTCTCAAACCCTAAACCCCCATCTCCTGGCACTCTCGCACCGGTCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCATCTCCTCACCCCAGTCCTCGTCACCTCATTCCAAACCCTAGCTTGTCGCTATGGTCCCCTCATCGAGCTCCGACTCGGCGCATCAAAGTGTGTCGTCGTCTCAACCGCTGCAGTCGCCAAAGACATCCTAAAAACCCACGAACACAACTTCCTCTCTCGACCGGAGTTCGGCGCCTCCGAGTACTTCATTTACCGAGGGTCGAGATTCGTCATGGCTCAATACGGACCCTATTGGCGATTCATGAAGAAGCTAACCATGACACGGCTTCTCTCGCCGCCGCAGTTGGCGGTCTCGACCGCCATCCGTAGCGACGAGATAGCAAAACTCGTGGAAAGAATAGAGGCAAATTCAAGGGAAAAGAAGCCCACAGATTTGAGATTGGAGTCTACTACTTTGACAAACAATATCATATCAAGGATGGTGTTTAGCACAAGGTGCTGTGGAGGGAAAGATGAGGCTCAAGAGATTAAGGATTTGGCTTGGAGGATCAATATGTTGGCTGGGAAGTTGAGTTTGGGTGATATTTTGGGACCTTTAAAAGTGTTTGATTTCTCCGGAAATGGAAAGAAATTTGTGAAAACTTTGAAGAAATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCTGCCAATAATGGTGCTGTGCTATGGGCAATGGGAGAGCTTCTAAATCGTCCAGAATCGATCCAAAAGCTAAGAAAAGAGATAATATCAGTGGTTGGAAACAAGAAGCAGGTCCAAGAATCCGACCTCCCCAACCTCCCATATCTCCGAGCAGTGGTCAATGAAACACTCCGTCTCCACCCGTCGGCGCCAATCATCATCAGGGAATGTTTAGATGACTGTTACATCAATGGCTCACTAATCAAGGCCAAAACTCGAGTATTGGTCAATGCATATGCCGTCATGAGAGACCCAGAGTCTTGGTCAGAGCCTGACAGGTTCTTGCCAGAGAGGTTTTTGGAAGGCTCCCATGAGAATATCGGAGGCCATCGGATGGAGATGAAAGGGCAGAATTTTCGTTACATTCCGTTTGGGAGTGGAAAGAGAGGCTGTCCTGGATCTTCCCTTGCATTGTTAGTTTTTCCATGTGCAATTGCGACCATGGTGCAACGATTTGATTGGAAGACAGGTGGGAATGACAGTGGCAACATTGATTTGACACTTGGGTCGGGATTTGCTGCAGAGATGGCAACGCCACTTGTCTGTTATGCAAACTCCATTGATGGGAGTGATTAA

Coding sequence (CDS)

ATGACCTCCATCTTTGACAACACCACCTCTCCCTCTCAATTTTGGTGCTTCTGGTTCATCATTGCCCTCCTCCTCCATCTCCTCCTCAAAAAACATCTCTCAAACCCTAAACCCCCATCTCCTGGCACTCTCGCACCGGTCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCATCTCCTCACCCCAGTCCTCGTCACCTCATTCCAAACCCTAGCTTGTCGCTATGGTCCCCTCATCGAGCTCCGACTCGGCGCATCAAAGTGTGTCGTCGTCTCAACCGCTGCAGTCGCCAAAGACATCCTAAAAACCCACGAACACAACTTCCTCTCTCGACCGGAGTTCGGCGCCTCCGAGTACTTCATTTACCGAGGGTCGAGATTCGTCATGGCTCAATACGGACCCTATTGGCGATTCATGAAGAAGCTAACCATGACACGGCTTCTCTCGCCGCCGCAGTTGGCGGTCTCGACCGCCATCCGTAGCGACGAGATAGCAAAACTCGTGGAAAGAATAGAGGCAAATTCAAGGGAAAAGAAGCCCACAGATTTGAGATTGGAGTCTACTACTTTGACAAACAATATCATATCAAGGATGGTGTTTAGCACAAGGTGCTGTGGAGGGAAAGATGAGGCTCAAGAGATTAAGGATTTGGCTTGGAGGATCAATATGTTGGCTGGGAAGTTGAGTTTGGGTGATATTTTGGGACCTTTAAAAGTGTTTGATTTCTCCGGAAATGGAAAGAAATTTGTGAAAACTTTGAAGAAATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCTGCCAATAATGGTGCTGTGCTATGGGCAATGGGAGAGCTTCTAAATCGTCCAGAATCGATCCAAAAGCTAAGAAAAGAGATAATATCAGTGGTTGGAAACAAGAAGCAGGTCCAAGAATCCGACCTCCCCAACCTCCCATATCTCCGAGCAGTGGTCAATGAAACACTCCGTCTCCACCCGTCGGCGCCAATCATCATCAGGGAATGTTTAGATGACTGTTACATCAATGGCTCACTAATCAAGGCCAAAACTCGAGTATTGGTCAATGCATATGCCGTCATGAGAGACCCAGAGTCTTGGTCAGAGCCTGACAGGTTCTTGCCAGAGAGGTTTTTGGAAGGCTCCCATGAGAATATCGGAGGCCATCGGATGGAGATGAAAGGGCAGAATTTTCGTTACATTCCGTTTGGGAGTGGAAAGAGAGGCTGTCCTGGATCTTCCCTTGCATTGTTAGTTTTTCCATGTGCAATTGCGACCATGGTGCAACGATTTGATTGGAAGACAGGTGGGAATGACAGTGGCAACATTGATTTGACACTTGGGTCGGGATTTGCTGCAGAGATGGCAACGCCACTTGTCTGTTATGCAAACTCCATTGATGGGAGTGATTAA

Protein sequence

MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREKKPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNGAVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQRFDWKTGGNDSGNIDLTLGSGFAAEMATPLVCYANSIDGSD

Homology

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BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.6e-85
Identity = 179/474 (37.76%), Postives = 260/474 (54.85%), Query Frame = 0


Query: 48  PSPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHE 107
           PSP ALP+IGHLHL++P+    F  L+ R+GP+++L LG+  CVV STA  AK+ LKTHE
Sbjct: 31  PSPKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQLFLGSVPCVVASTAEAAKEFLKTHE 90

Query: 108 HNFLSRPEFGASEYFIYRGSR---FVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSD 167
            NF +RP    +   +   S+   F  A +GPYW+FMKKL M+ LLS   +     +R  
Sbjct: 91  INFSNRPGQNVAVKGLAYDSQDFLFAFAPFGPYWKFMKKLCMSELLSGRMMDQFLPVRQQ 150

Query: 168 EIAKLVERIEANSREKKPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRIN 227
           E  + + R+       +  D   E  TL+NNI+SRM  S +     ++A+E+K L   I 
Sbjct: 151 ETKRFISRVFRKGVAGEAVDFGDELMTLSNNIVSRMTLSQKTSENDNQAEEMKKLVSNIA 210

Query: 228 MLAGKLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMKE---------------- 287
            L GK ++ D +  LK FD  G  +K  +T  +FD +V+ I+K+                
Sbjct: 211 ELMGKFNVSDFIWYLKPFDLQGFNRKIKETRDRFDVVVDGIIKQRQEERRKNKETGTAKQ 270

Query: 288 -----------HEAANNG-------------------------AVLWAMGELLNRPESIQ 347
                      HE  N                           ++ WAM EL+N P+ ++
Sbjct: 271 FKDMLDVLLDMHEDENAEIKLDKKNIKAFIMDIFVAGTDTSAVSIEWAMAELINNPDVLE 330

Query: 348 KLRKEIISVVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIK 407
           K R+EI +VVG  + V+ESD+ NLPYL+A+V ETLRLHP  P+++RE      + G  I 
Sbjct: 331 KARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLRLHPGGPLVVRESSKSAVVCGYDIP 390

Query: 408 AKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKR 466
           AKTR+ VN +A+ RDP  W +P  F PERF+       G ++++++GQ++ +IPFGSG+R
Sbjct: 391 AKTRLFVNVWAIGRDPNHWEKPFEFRPERFIRD-----GQNQLDVRGQHYHFIPFGSGRR 450

[Close]

BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot
Match: Q9XGT9 (Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 5.4e-81
Identity = 183/503 (36.38%), Postives = 265/503 (52.68%), Query Frame = 0


Query: 14  FWCFWFIIALLLHLL-LKKHLSNPKPPSPGTLAPVPSPPALPLIGHLHLLTPVLVTSFQT 73
           F  F+F   L L+ L  K++ ++ + P        PSPP+ P+IGHLH L P++  SF  
Sbjct: 8   FLLFFFPTLLFLYCLPYKRNQNHRRLP--------PSPPSFPIIGHLHHLGPLIHQSFHA 67

Query: 74  LACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASEYFIYRGSRFVMA 133
           L+ RYG LI LRLG+  CVVVST  +AKD LKT+E  F SR    A ++  Y G  F  A
Sbjct: 68  LSTRYGSLIHLRLGSVPCVVVSTPDLAKDFLKTNELAFSSRKHSLAIDHITY-GVAFAFA 127

Query: 134 QYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREKKPTDLRLESTTL 193
            YG YW+F+KKL    LL    L+    IR+ EI +L+  +   SR K+  +L  E   L
Sbjct: 128 PYGTYWKFIKKLFTVELLGTQNLSHFLPIRTHEIRELLRTLMVKSRAKERVNLTEELLKL 187

Query: 194 TNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLKVFDFSGNGKKFV 253
           TNN+IS+M+ S RC G   EA E K+L   +  + G+ ++ D +   K  D  G  K++ 
Sbjct: 188 TNNVISQMMMSIRCSGTNSEADEAKNLVREVTKIFGQFNVSDFIWFCKNIDLQGFKKRYE 247

Query: 254 KTLKKFDGLVER-IMKEHEAANNG------------------------------------ 313
            T +++D L+ER IM   E    G                                    
Sbjct: 248 GTHRRYDALLERIIMGREENRRRGKIKDGEGKDFLDMLLDVLEDGKAEIKITRDHIKALI 307

Query: 314 -------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDLPNLPYLRAV 373
                        A+ WA+ EL+N P +++K R+EI  V+G+++ VQESD PNLPY++A+
Sbjct: 308 LDFLTAGTDTTAIAIEWALVELINNPNALEKARQEIDQVIGDERLVQESDTPNLPYIQAI 367

Query: 374 VNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSEPDRFLPERF 433
           + E LRLHP  P++IR+  ++  + G  I A T + VN +++ R+P+ W  P  F P RF
Sbjct: 368 IKEALRLHPPIPMLIRKSTENVIVQGYDIPAGTLLFVNIWSIGRNPQCWETPLEFKPHRF 427

Query: 434 LEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQRFDWKTGGN 466
           L+G         +++KG NF+ +PFG+G+RGCPG +LA+      IA ++Q FDW   G 
Sbjct: 428 LDGGDLK---SSLDIKGHNFQLLPFGTGRRGCPGVNLAMRELSVVIANLIQCFDWDVVGE 487

[Close]

BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.6e-80
Identity = 180/477 (37.74%), Postives = 253/477 (53.04%), Query Frame = 0


Query: 43  TLAPVPSPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDI 102
           TL P PSP  LP+IGHLHLL+P     F  L+ RYGP+I L LG+  CVV STA  AK+ 
Sbjct: 29  TLLP-PSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEF 88

Query: 103 LKTHEHNFLSRP-EFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAI 162
           LKTHE  F +RP    A E   Y    F+ A YGPYW+FMKKL M+ LL    L     +
Sbjct: 89  LKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPV 148

Query: 163 RSDEIAKLVERIEANSREKKPTDLRLESTTLTNNIISRMVFS-TRCCGGKDEAQEIKDLA 222
           R  E  K ++R+       +  D   E  TL+NNI+SRM+ S T     ++E +E++ L 
Sbjct: 149 RQXETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLV 208

Query: 223 WRINMLAGKLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMKEHE---------- 282
                L+GK ++ D +  LK FD  G  K+  K    FD +++RI+K+ E          
Sbjct: 209 KDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETV 268

Query: 283 ----------------------------------------AANNGAVL--WAMGELLNRP 342
                                                     +  AV   WAM EL+N P
Sbjct: 269 GKREFKDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNP 328

Query: 343 ESIQKLRKEIISVVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYING 402
             ++K R+E+ +VVG  + V+ESD+ NLPYL+ +V ETLRLHP+ P++ RE      + G
Sbjct: 329 GVLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRESSRRAVVCG 388

Query: 403 SLIKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFG 462
             I AKTR+ VN +A+ RDP  W  P  F PERF+E      G  +++++GQ++  +PFG
Sbjct: 389 YDIPAKTRLFVNVWAIGRDPNHWENPLEFRPERFVEN-----GKSQLDVRGQHYHLLPFG 448

Query: 463 SGKRGCPGSSLALLVFPCAIATMVQRFDWKTGGNDSGNIDLTLGSGFAAEMATPLVC 466
           SG+R CPG+SLAL V    +A ++Q F WK    D+G +++   +G     A P++C
Sbjct: 449 SGRRACPGTSLALQVVHVNLAVLIQCFQWKV-DCDNGKVNMEEKAGITLPRAHPIIC 498

[Close]

BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.5e-80
Identity = 172/499 (34.47%), Postives = 259/499 (51.90%), Query Frame = 0


Query: 18  WFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLHLLTPVLVTSFQTLACRY 77
           WFI  +L+  + KK      PP P        P ++PL+GH   L  +L  +   L+ RY
Sbjct: 14  WFISTILIRSIFKKPQRLRLPPGP--------PISIPLLGHAPYLRSLLHQALYKLSLRY 73

Query: 78  GPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASEYFIYRGSRFVMAQYGPY 137
           GPLI + +G+   VV S+A  AK ILKT E  F +RP   ASE   Y  + +    YG Y
Sbjct: 74  GPLIHVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAADYFFIPYGTY 133

Query: 138 WRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVER-IEANSREKKPTDLRLESTTLTNNI 197
           WRF+KKL MT LLS   L     IR  E+   ++R +E +        +R E  T TNNI
Sbjct: 134 WRFLKKLCMTELLSGKTLEHFVRIRESEVEAFLKRMMEISGNGNYEVVMRKELITHTNNI 193

Query: 198 ISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLKVFDFSGNGKKFVKTLK 257
           I+RM+   +     DE   ++ +   +  L G  +LGD++G ++  D  G GKK ++T  
Sbjct: 194 ITRMIMGKKSNAENDEVARLRKVVREVGELLGAFNLGDVIGFMRPLDLQGFGKKNMETHH 253

Query: 258 KFDGLVERIMKEHE---------------------------------------------- 317
           K D ++E++++EHE                                              
Sbjct: 254 KVDAMMEKVLREHEEARAKEDADSDRKKDLFDILLNLIEADGADNKLTRESAKAFALDMF 313

Query: 318 -AANNG---AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDLPNLPYLRAVVNET 377
            A  NG    + W++ EL+  P   +K R+EI SVVG ++ V+ESD+PNLPYL+AV+ ET
Sbjct: 314 IAGTNGPASVLEWSLAELVRNPHVFKKAREEIESVVGKERLVKESDIPNLPYLQAVLKET 373

Query: 378 LRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGS 437
           LRLHP  PI  RE +  C + G  I   + +L++ +A+ RDP  W +   + PERFL   
Sbjct: 374 LRLHPPTPIFAREAMRTCQVEGYDIPENSTILISTWAIGRDPNYWDDALEYKPERFL--F 433

Query: 438 HENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQRFDWKTGGNDSGN 466
            ++ G  +++++GQ ++ +PFGSG+R CPG+SLALLV    +A+++Q FDW      + +
Sbjct: 434 SDDPGKSKIDVRGQYYQLLPFGSGRRSCPGASLALLVMQATLASLIQCFDWIVNDGKNHH 493

[Close]

BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot
Match: Q42799 (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-79
Identity = 172/470 (36.60%), Postives = 249/470 (52.98%), Query Frame = 0


Query: 48  PSPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHE 107
           PSP  LP+IGHLHL++P+    F  L+ R+GP+++L LG+  CVV STA  AK+ LKTHE
Sbjct: 31  PSPKGLPIIGHLHLVSPIPHQDFYKLSLRHGPIMQLFLGSVPCVVASTAEAAKEFLKTHE 90

Query: 108 HNFLSRPEFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIA 167
            NF +RP    +  F+     +V   YGP  +F+KKL M+ LL    L     +R  E  
Sbjct: 91  INFSNRPGQNVAVQFL----TYVFGPYGPSVKFIKKLCMSELLGGRMLDQFLPVRQQETK 150

Query: 168 KLVERIEANSREKKPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLA 227
           K ++R+       +  D   E   L+NNIISRM  +      + +A+E++ L   +  L 
Sbjct: 151 KFIKRVLQKGIAGEAVDFGGEFMRLSNNIISRMTMNQTSSEDEKQAEEMRMLVADVAELM 210

Query: 228 GKLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMK--EHEAANN----------- 287
           G  ++ D +  LK FD  G  K+  KT  +FD +++RI+K  E E  NN           
Sbjct: 211 GTFNVSDFIWFLKPFDLQGFNKRIRKTRIRFDAVLDRIIKQREEERRNNKEIGGTRQFKD 270

Query: 288 ---------------------------------------GAVLWAMGELLNRPESIQKLR 347
                                                    + WAM EL+N P  ++K R
Sbjct: 271 ILDVLLDIGEDDSSEIKLTKENIKAFIMDIFVAGTDTSAATMEWAMAELINNPCVLEKAR 330

Query: 348 KEIISVVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKT 407
           +EI +VVGN + ++ESD+ NLPYL+A+V ETLR+HP  P+I+RE      + G  I AKT
Sbjct: 331 QEIDAVVGNSRIIEESDIVNLPYLQAIVRETLRIHPGGPLIVRESSKSVVVCGYEIPAKT 390

Query: 408 RVLVNAYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCP 466
           R+ VN +A+ RDP  W  P  F PERF E      G  +++++GQ++ +IPFGSG+R CP
Sbjct: 391 RLFVNVWAIGRDPNHWENPFEFRPERFFEN-----GQSQLDVRGQHYHFIPFGSGRRSCP 450

[Close]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL
Match: A0A1S3B805 (LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103486788 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.5e-235
Identity = 419/517 (81.04%), Postives = 442/517 (85.49%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW FWFI ALLLHLLLKK L+  KP SP T  P PSPPALPLIGHLH
Sbjct: 18  MTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIGHLH 77

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPV+VTSFQTLA RYGPLIE+RLGASKCV+VSTAAVAK+ILKTHEHNFLSRPEFGASE
Sbjct: 78  LLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFGASE 137

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEI KLVERIEA+SRE 
Sbjct: 138 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASSREG 197

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+D+RLE TTLTNNIISRM+ STRCCGGKDEA+EIK+LAWRINMLAGKLSLGDILG LK
Sbjct: 198 KPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILGALK 257

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           VFDFSGNGKKFV+TLKKFDGLVERIMKEHEAA +                          
Sbjct: 258 VFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSGDEERKDLLDILLEIYNDPNADMKI 317

Query: 301 ----------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDL 360
                                 A+LWAMGELLN PES+QKLRKEI SVVGNKK VQESDL
Sbjct: 318 TRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESDL 377

Query: 361 PNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSE 420
           PNLPYLRA+VNETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESWSE
Sbjct: 378 PNLPYLRAIVNETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWSE 437

Query: 421 PDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQ 471
           PD+FLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIATMVQ
Sbjct: 438 PDKFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMVQ 497

[Close]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL
Match: A0A5D3DP65 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005640 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 2.0e-235
Identity = 419/518 (80.89%), Postives = 442/518 (85.33%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW FWFI ALLLHLLLKK L+  KP SP T  P PSPPALPLIGHLH
Sbjct: 4   MTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPV+VTSFQTLA RYGPLIE+RLGASKCV+VSTAAVAK+ILKTHEHNFLSRPEFGASE
Sbjct: 64  LLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEI KLVERIEA+SRE 
Sbjct: 124 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASSREG 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+D+RLE TTLTNNIISRM+ STRCCGGKDEA+EIK+LAWRINMLAGKLSLGDILG LK
Sbjct: 184 KPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILGALK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           VFDFSGNGKKFV+TLKKFDGLVERIMKEHEAA +                          
Sbjct: 244 VFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSGDEERKKDLLDILLEIYNDPNADMK 303

Query: 301 -----------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESD 360
                                  A+LWAMGELLN PES+QKLRKEI SVVGNKK VQESD
Sbjct: 304 ITRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESD 363

Query: 361 LPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWS 420
           LPNLPYLRA+VNETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESWS
Sbjct: 364 LPNLPYLRAIVNETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWS 423

Query: 421 EPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMV 471
           EPD+FLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIATMV
Sbjct: 424 EPDKFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMV 483

[Close]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL
Match: A0A6J1J5T8 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111481610 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.6e-223
Identity = 401/523 (76.67%), Postives = 427/523 (81.64%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           M +IF N TSPSQFWCFWFI ALLLH  LKK L NPK   PG  AP PSPPALPLIGHLH
Sbjct: 4   MATIFHNITSPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGHAPPPSPPALPLIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQTLA RYG LIELRLGASKCVVVSTAAVAK++LKTHEHNFLSRPEFGASE
Sbjct: 64  LLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTAAVAKEVLKTHEHNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           +FIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA++ AIR DE+ KLVERIEANSR K
Sbjct: 124 HFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANSRGK 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+DLRLE TTLTNNIISRM+ STRCC G DEAQE++DL W+IN LAGKLSLGDILGPLK
Sbjct: 184 KPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPLK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           V DFSGNGKKFV T+K+FDGLVERIMKEHEAA NG                         
Sbjct: 244 VLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAINGGGHPDPDRKLDVLDILLECHNDPHA 303

Query: 301 --------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQ 360
                                     A+LWAM ELLNRP+S+++LRKEIISVVG +K VQ
Sbjct: 304 EMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLVQ 363

Query: 361 ESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPE 420
           ESDLPNLPYLRAVVNETLRLHPSAP+IIRECLDDC INGSL+KAKTRVLVNAYAVMRDPE
Sbjct: 364 ESDLPNLPYLRAVVNETLRLHPSAPMIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPE 423

Query: 421 SWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIA 472
           SWSEPDRFLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+A
Sbjct: 424 SWSEPDRFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMA 483

[Close]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL
Match: A0A6J1F1K7 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111441548 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.0e-220
Identity = 398/521 (76.39%), Postives = 424/521 (81.38%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           M +IF N TS S FWCFWFI ALLLH  LKK L NPK   PG  A  PSPPALPLIGHLH
Sbjct: 4   MVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQTLA RYG LIELRLGASKCVVVSTA VAK++LKTHEHNFLSRPEFGASE
Sbjct: 64  LLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA++ AIR DE+ KLVERIEANSREK
Sbjct: 124 YFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANSREK 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+DLRLE TTLTNNIISRM+ STRCC G DEAQE++DL W+IN LAGKLSLGDILGPLK
Sbjct: 184 KPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPLK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           V DFSGNGKKFV T+K+FDGLVERIMKEHEAA NG                         
Sbjct: 244 VLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAVNGGRDPDRKLDVLDILLECHNDPNAEM 303

Query: 301 ------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQES 360
                                   A+LWAM ELLNRP+S+++LRKEIISVVG KK VQES
Sbjct: 304 NISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLVQES 363

Query: 361 DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESW 420
           DLPNLPYLRAVVNETLRLHPSAP+IIRECLDDC ING+L+KAKTRVLVNAYAVMRDPESW
Sbjct: 364 DLPNLPYLRAVVNETLRLHPSAPLIIRECLDDCIINGTLVKAKTRVLVNAYAVMRDPESW 423

Query: 421 SEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATM 472
           SEPDRFLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+A M
Sbjct: 424 SEPDRFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAM 483

[Close]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL
Match: A0A0A0LBD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 1.0e-175
Identity = 331/431 (76.80%), Postives = 349/431 (80.97%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW F FI  LL HLLL+K L+  +P SP T  P PSP ALP IGHLH
Sbjct: 4   MTTIFDNTTSPSQFWSFCFITILLFHLLLRKLLTKSRPSSPDTPKPPPSPSALPFIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQTLA RYGPLIE+RLGASKC+VVSTA VAK+ILKTHE NFLSRPEFGASE
Sbjct: 64  LLTPVLVTSFQTLARRYGPLIEVRLGASKCIVVSTATVAKEILKTHEGNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRS+EIAKLVERIEA+SRE+
Sbjct: 124 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSEEIAKLVERIEASSREE 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP DLRLE TTLTNNIISRMV STRCCGGKDEA+EIKDLAWRINMLAGKLSLGDILG LK
Sbjct: 184 KPLDLRLEFTTLTNNIISRMVLSTRCCGGKDEAKEIKDLAWRINMLAGKLSLGDILGALK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAA-NNG------------------------ 300
           VFDFSGNGKKFVKTLKKFD L+ERIMKEHEAA N+G                        
Sbjct: 244 VFDFSGNGKKFVKTLKKFDELIERIMKEHEAAINDGEDEERKKDLLDILLEIYNDPNADF 303

Query: 301 ------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQES 360
                                   A+LWAMGELLN PES  +LR EI SVVGNKK VQES
Sbjct: 304 KLTRNDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESFHQLRNEITSVVGNKKPVQES 363

Query: 361 DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESW 383
           DLPNLPYL+AVV ETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESW
Sbjct: 364 DLPNLPYLQAVVKETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESW 423

[Close]

BLAST of Lsi03G017140 vs. NCBI nr
Match: XP_038905529.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida])

HSP 1 Score: 828.9 bits (2140), Expect = 2.2e-236
Identity = 422/523 (80.69%), Postives = 441/523 (84.32%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           +T+IFDNTTS SQFWCFWFIIA LLHL LKKHL  PKP S G   P PSPPALPLIGHLH
Sbjct: 20  ITAIFDNTTSTSQFWCFWFIIAFLLHLFLKKHLPKPKPSSLGGPTPPPSPPALPLIGHLH 79

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQ LA RYGPLIELRLGASKCVVVS AAVAK+ILKTHEHNFLSRPEFGASE
Sbjct: 80  LLTPVLVTSFQALARRYGPLIELRLGASKCVVVSNAAVAKEILKTHEHNFLSRPEFGASE 139

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKL+ERIE NSRE 
Sbjct: 140 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLMERIEGNSREN 199

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP D+RLE TTLTNNIISRMV STRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK
Sbjct: 200 KPIDMRLEFTTLTNNIISRMVLSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 259

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           VFDFSGNGKKFVKTLK+FD LVE+IMKEHEAANNG                         
Sbjct: 260 VFDFSGNGKKFVKTLKRFDELVEKIMKEHEAANNGDGDEERRRDLLDILLEIYKDPNAEM 319

Query: 301 ------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQES 360
                                   A+LWAMGELLNR ES+Q+LRKEI SV+GNKK V+ES
Sbjct: 320 KITKNDIKSFLLDLFMAGTDTTATAILWAMGELLNRRESLQELRKEITSVIGNKKMVEES 379

Query: 361 DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESW 420
           DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDC ING LIKAKTRVLVNAYAVM+DPESW
Sbjct: 380 DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCNINGLLIKAKTRVLVNAYAVMKDPESW 439

Query: 421 SEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATM 475
           S+PD+FLPERFL+GS +NIG HRMEMKGQNFRYIPFGSGKRGCPG+SLALLVFPCAIATM
Sbjct: 440 SDPDKFLPERFLKGSLDNIGTHRMEMKGQNFRYIPFGSGKRGCPGASLALLVFPCAIATM 499

[Close]

BLAST of Lsi03G017140 vs. NCBI nr
Match: XP_008443083.1 (PREDICTED: LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo])

HSP 1 Score: 825.1 bits (2130), Expect = 3.1e-235
Identity = 419/517 (81.04%), Postives = 442/517 (85.49%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW FWFI ALLLHLLLKK L+  KP SP T  P PSPPALPLIGHLH
Sbjct: 18  MTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIGHLH 77

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPV+VTSFQTLA RYGPLIE+RLGASKCV+VSTAAVAK+ILKTHEHNFLSRPEFGASE
Sbjct: 78  LLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFGASE 137

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEI KLVERIEA+SRE 
Sbjct: 138 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASSREG 197

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+D+RLE TTLTNNIISRM+ STRCCGGKDEA+EIK+LAWRINMLAGKLSLGDILG LK
Sbjct: 198 KPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILGALK 257

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           VFDFSGNGKKFV+TLKKFDGLVERIMKEHEAA +                          
Sbjct: 258 VFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSGDEERKDLLDILLEIYNDPNADMKI 317

Query: 301 ----------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDL 360
                                 A+LWAMGELLN PES+QKLRKEI SVVGNKK VQESDL
Sbjct: 318 TRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESDL 377

Query: 361 PNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSE 420
           PNLPYLRA+VNETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESWSE
Sbjct: 378 PNLPYLRAIVNETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWSE 437

Query: 421 PDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQ 471
           PD+FLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIATMVQ
Sbjct: 438 PDKFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMVQ 497

[Close]

BLAST of Lsi03G017140 vs. NCBI nr
Match: KAA0043713.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1 3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 824.7 bits (2129), Expect = 4.1e-235
Identity = 419/518 (80.89%), Postives = 442/518 (85.33%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW FWFI ALLLHLLLKK L+  KP SP T  P PSPPALPLIGHLH
Sbjct: 4   MTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPV+VTSFQTLA RYGPLIE+RLGASKCV+VSTAAVAK+ILKTHEHNFLSRPEFGASE
Sbjct: 64  LLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEI KLVERIEA+SRE 
Sbjct: 124 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASSREG 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+D+RLE TTLTNNIISRM+ STRCCGGKDEA+EIK+LAWRINMLAGKLSLGDILG LK
Sbjct: 184 KPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILGALK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           VFDFSGNGKKFV+TLKKFDGLVERIMKEHEAA +                          
Sbjct: 244 VFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSGDEERKKDLLDILLEIYNDPNADMK 303

Query: 301 -----------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESD 360
                                  A+LWAMGELLN PES+QKLRKEI SVVGNKK VQESD
Sbjct: 304 ITRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQESD 363

Query: 361 LPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWS 420
           LPNLPYLRA+VNETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESWS
Sbjct: 364 LPNLPYLRAIVNETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESWS 423

Query: 421 EPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMV 471
           EPD+FLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFP AIATMV
Sbjct: 424 EPDKFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPRAIATMV 483

[Close]

BLAST of Lsi03G017140 vs. NCBI nr
Match: XP_004136741.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis sativus] >KAE8651142.1 hypothetical protein Csa_001178 [Cucumis sativus])

HSP 1 Score: 795.8 bits (2054), Expect = 2.0e-226
Identity = 409/521 (78.50%), Postives = 434/521 (83.30%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           MT+IFDNTTSPSQFW F FI  LL HLLL+K L+  +P SP T  P PSP ALP IGHLH
Sbjct: 4   MTTIFDNTTSPSQFWSFCFITILLFHLLLRKLLTKSRPSSPDTPKPPPSPSALPFIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQTLA RYGPLIE+RLGASKC+VVSTA VAK+ILKTHE NFLSRPEFGASE
Sbjct: 64  LLTPVLVTSFQTLARRYGPLIEVRLGASKCIVVSTATVAKEILKTHEGNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRS+EIAKLVERIEA+SRE+
Sbjct: 124 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSEEIAKLVERIEASSREE 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP DLRLE TTLTNNIISRMV STRCCGGKDEA+EIKDLAWRINMLAGKLSLGDILG LK
Sbjct: 184 KPLDLRLEFTTLTNNIISRMVLSTRCCGGKDEAKEIKDLAWRINMLAGKLSLGDILGALK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAA-NNG------------------------ 300
           VFDFSGNGKKFVKTLKKFD L+ERIMKEHEAA N+G                        
Sbjct: 244 VFDFSGNGKKFVKTLKKFDELIERIMKEHEAAINDGEDEERKKDLLDILLEIYNDPNADF 303

Query: 301 ------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQES 360
                                   A+LWAMGELLN PES  +LR EI SVVGNKK VQES
Sbjct: 304 KLTRNDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESFHQLRNEITSVVGNKKPVQES 363

Query: 361 DLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESW 420
           DLPNLPYL+AVV ETLRLHPSAPIIIRECLDDC IN SLIKAKTRVL+NAYAVMRDPESW
Sbjct: 364 DLPNLPYLQAVVKETLRLHPSAPIIIRECLDDCNINDSLIKAKTRVLINAYAVMRDPESW 423

Query: 421 SEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATM 473
           SEPD+FLPERFLEGSHENIG H+MEMKGQNFRYIPFGSGKRGCPG+SLALLVFPCAIATM
Sbjct: 424 SEPDKFLPERFLEGSHENIGSHKMEMKGQNFRYIPFGSGKRGCPGASLALLVFPCAIATM 483

[Close]

BLAST of Lsi03G017140 vs. NCBI nr
Match: XP_022982904.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima])

HSP 1 Score: 782.7 bits (2020), Expect = 1.8e-222
Identity = 401/523 (76.67%), Postives = 427/523 (81.64%), Query Frame = 0


Query: 1   MTSIFDNTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLH 60
           M +IF N TSPSQFWCFWFI ALLLH  LKK L NPK   PG  AP PSPPALPLIGHLH
Sbjct: 4   MATIFHNITSPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGHAPPPSPPALPLIGHLH 63

Query: 61  LLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASE 120
           LLTPVLVTSFQTLA RYG LIELRLGASKCVVVSTAAVAK++LKTHEHNFLSRPEFGASE
Sbjct: 64  LLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTAAVAKEVLKTHEHNFLSRPEFGASE 123

Query: 121 YFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREK 180
           +FIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA++ AIR DE+ KLVERIEANSR K
Sbjct: 124 HFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANSRGK 183

Query: 181 KPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLK 240
           KP+DLRLE TTLTNNIISRM+ STRCC G DEAQE++DL W+IN LAGKLSLGDILGPLK
Sbjct: 184 KPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPLK 243

Query: 241 VFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNG------------------------- 300
           V DFSGNGKKFV T+K+FDGLVERIMKEHEAA NG                         
Sbjct: 244 VLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAINGGGHPDPDRKLDVLDILLECHNDPHA 303

Query: 301 --------------------------AVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQ 360
                                     A+LWAM ELLNRP+S+++LRKEIISVVG +K VQ
Sbjct: 304 EMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLVQ 363

Query: 361 ESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPE 420
           ESDLPNLPYLRAVVNETLRLHPSAP+IIRECLDDC INGSL+KAKTRVLVNAYAVMRDPE
Sbjct: 364 ESDLPNLPYLRAVVNETLRLHPSAPMIIRECLDDCIINGSLVKAKTRVLVNAYAVMRDPE 423

Query: 421 SWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIA 472
           SWSEPDRFLPERFLEGSHENIG HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+A
Sbjct: 424 SWSEPDRFLPERFLEGSHENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMA 483

[Close]

BLAST of Lsi03G017140 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 513.5 bits (1321), Expect = 1.9e-145
Identity = 263/470 (55.96%), Postives = 330/470 (70.21%), Query Frame = 0


Query: 49  SPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEH 108
           SPPALP IGHLHL+  VL  SFQ+LA +YGPL+E+RLGASKCVVVS+++VA++I K  E 
Sbjct: 44  SPPALPFIGHLHLIGKVLPVSFQSLAHKYGPLMEIRLGASKCVVVSSSSVAREIFKEQEL 103

Query: 109 NFLSRPEFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAK 168
           NF SRPEFG++EYF YRGSRFV+AQYG YWRFMKKL MT+LL+ PQL     IR +E  K
Sbjct: 104 NFSSRPEFGSAEYFKYRGSRFVLAQYGDYWRFMKKLCMTKLLAVPQLEKFADIREEEKLK 163

Query: 169 LVERIEANSREKKPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAG 228
           LV+ +    RE  P DL  +    TNN+I RM  STRC G  +EA+EI++L  +   LAG
Sbjct: 164 LVDSVAKCCREGLPCDLSSQFIKYTNNVICRMAMSTRCSGTDNEAEEIRELVKKSLELAG 223

Query: 229 KLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMKEHEA----------------- 288
           K+S+GD+LGPLKV DFSGNGKK V  ++K+D LVERIMKE EA                 
Sbjct: 224 KISVGDVLGPLKVMDFSGNGKKLVAVMEKYDLLVERIMKEREAKAKKKDGTRKDILDILL 283

Query: 289 ------------------------------ANNGAVLWAMGELLNRPESIQKLRKEIISV 348
                                          +  A+ WAMG+L+N P++  KLR+EI +V
Sbjct: 284 ETYRDPTAEMKITRNDMKSFLLDVFMAGTDTSAAAMQWAMGQLINHPQAFNKLREEINNV 343

Query: 349 VGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNA 408
           VG+K+ V+ESD+PNLPYLRAV+ ETLRLHPSAP+IIREC +DC +NG L+K+KTRVLVN 
Sbjct: 344 VGSKRLVKESDVPNLPYLRAVLRETLRLHPSAPLIIRECAEDCQVNGCLVKSKTRVLVNV 403

Query: 409 YAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLAL 468
           YA+MRD E W++ DRF+PERFLE S E IG H+M+ KGQNFRY+PFGSG+RGCPG+SLA+
Sbjct: 404 YAIMRDSELWADADRFIPERFLESSEEKIGEHQMQFKGQNFRYLPFGSGRRGCPGASLAM 463

Query: 469 LVFPCAIATMVQRFDWKTGGNDSGNIDLTLGSGFAAEMATPLVCYANSID 472
            V    + ++VQRFDWK+   D   +DL+ GSGF+AEMA PLVC  N +D
Sbjct: 464 NVMHIGVGSLVQRFDWKS--VDGQKVDLSQGSGFSAEMARPLVC--NPVD 509

[Close]

BLAST of Lsi03G017140 vs. TAIR 10
Match: AT5G06905.1 (cytochrome P450, family 712, subfamily A, polypeptide 2 )

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-86
Identity = 192/523 (36.71%), Postives = 277/523 (52.96%), Query Frame = 0


Query: 7   NTTSPSQFWCFWFIIALLLHLLLKKHLSNPKPPSPGTLAPVPSPPALPLIGHLHLLTPVL 66
           N TS S  + F  +   LLH L         P         P P  LP++GH+HLL   L
Sbjct: 2   NYTSCSYLF-FTLVTIFLLHRLFSSSSRRGLP---------PGPRGLPVLGHMHLLRSSL 61

Query: 67  VTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASEYFIYRG 126
             S Q LA  YGPL+ +R+G+ + +VVS +  AK ILKTH+ +F S+  FG  ++ +Y+G
Sbjct: 62  PRSLQALAHTYGPLMTIRIGSLRVLVVSDSDTAKLILKTHDPDFASKFVFGPRQFNVYKG 121

Query: 127 SRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREKKPTDLR 186
           S F  A YG YWRFMKKL MT+L +  QL     IR +E   L+  +   SR  +  DL 
Sbjct: 122 SEFFNAPYGSYWRFMKKLCMTKLFAGYQLDRFVDIREEETLALLSTLVERSRNGEACDLG 181

Query: 187 LESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILGPLKVFDFSG 246
           LE T LT  I+S+MV   RC    +  +EI+ +   I   A +    ++ GPL+  D  G
Sbjct: 182 LEFTALTTKILSKMVMGKRCRQNSNIPKEIRKIVSDIMACATRFGFMELFGPLRDLDLFG 241

Query: 247 NGKKFVKTLKKFDGLVERIMKEHE------------------------------------ 306
           NGKK   ++ ++D LVE+I+KE+E                                    
Sbjct: 242 NGKKLRSSIWRYDELVEKILKEYENDKSNEEEEKDKDIVDILLDTYNDPKAELRLTMNQI 301

Query: 307 ------------AANNGAVLWAMGELLNRPESIQKLRKEIISVVG-NKKQVQESDLPNLP 366
                          + A+ W M EL+N P+   K+R EI SVVG   + ++ESDL  LP
Sbjct: 302 KFFILELFMASLDTTSAALQWTMTELINHPDIFAKIRDEIKSVVGTTNRLIKESDLQKLP 361

Query: 367 YLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSEPDRF 426
           YL+A + ETLRLHP  P++ RE   D  ING  +K+ T++ +NAY +MRDP ++ +PD+F
Sbjct: 362 YLQAAIKETLRLHPVGPLLRRESNTDMKINGYDVKSGTKIFINAYGIMRDPTTYKDPDKF 421

Query: 427 LPERFL---EGSHENIGGHR---MEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATM 467
           +PERFL   + +   +G ++   +E+KGQ+  Y+ FGSG+RGC G+S A LV    I ++
Sbjct: 422 MPERFLVVEQDTERKMGYYQQYMLELKGQDVNYLAFGSGRRGCLGASHASLVLSLTIGSL 481

[Close]

BLAST of Lsi03G017140 vs. TAIR 10
Match: AT2G14100.1 (cytochrome P450, family 705, subfamily A, polypeptide 13 )

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-82
Identity = 179/465 (38.49%), Postives = 250/465 (53.76%), Query Frame = 0


Query: 48  PSPPALPLIGHLHLLTPVLV-TSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTH 107
           PSPP+LP+IGHLHLL   L   +FQ L+ +YGPL+ LR+     V+VS+A+VA +I KTH
Sbjct: 45  PSPPSLPIIGHLHLLLFDLPHKAFQKLSSKYGPLLCLRIFNVPIVLVSSASVAYEIFKTH 104

Query: 108 EHNFLSRPEFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEI 167
           + N  S       E   +  S FVMA YG YW+FMKKL +T+L  P  L  S   R+DE+
Sbjct: 105 DVNISSHGHPPIDECLFFGSSSFVMAPYGDYWKFMKKLMVTKLFGPQALEQSRGARADEL 164

Query: 168 AKLVERIEANSREKKPTDLRLESTTLTNNIISRMVFSTRCCGGKDEAQEIKDLAWRINML 227
            +    + +   + +  ++  E+  LTNN I +M+    C     EA+ ++ L      L
Sbjct: 165 ERFHANLLSKEMKSETVEIAKEAIKLTNNSICKMIMGRGCLEENGEAERVRGLVTETFAL 224

Query: 228 AGKLSLGDILGPL-KVFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANN----------- 287
             KL L  +L  L ++   S   K+ +   +KFD L+ERI+ EHE   +           
Sbjct: 225 FKKLFLTQVLRRLFEILRISPFKKETLDVSRKFDELLERIIVEHEEKTDYDHGMDLMDVL 284

Query: 288 ----------------------------------GAVLWAMGELLNRPESIQKLRKEIIS 347
                                               + W M +++ +P  +++LRKEI S
Sbjct: 285 LAVYRDGKAEYKITRDHLKSLFVELILGGTDTSAQTIEWTMAKIIKKPNILERLRKEIDS 344

Query: 348 VVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLVN 407
           VVG  + +QE DLPNLPYL+AV+ E LRLHP AP++ R+  D C I G  +   T ++VN
Sbjct: 345 VVGKTRLIQEKDLPNLPYLQAVIKEGLRLHPPAPLLGRKVTDGCTIGGCYVPKNTTLVVN 404

Query: 408 AYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLA 466
           AYAVMRDP+SW +PD F PERFL  S       + E + Q  +YIPFGSG+RGCPG +L 
Sbjct: 405 AYAVMRDPDSWEDPDEFKPERFLASSR-----GKEEEREQELKYIPFGSGRRGCPGVNLG 464

[Close]

BLAST of Lsi03G017140 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 290.0 bits (741), Expect = 3.4e-78
Identity = 168/466 (36.05%), Postives = 254/466 (54.51%), Query Frame = 0


Query: 48  PSPPALPLIGHLHLLTPVLVTSFQTLACRYGPLIELRLGASKCVVVSTAAVAKDILKTHE 107
           PSP ALP+IGH+HLL P+   +   L+ RYGPL+ L +G+   ++VS+A +A +ILK++E
Sbjct: 36  PSPTALPIIGHIHLLGPIAHQALHKLSIRYGPLMYLFIGSIPNLIVSSAEMANEILKSNE 95

Query: 108 HNFLSRPEFGASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIA 167
            NFL+RP     +Y  Y  + F  A YG +W+FMK++ M  L S   L    ++RS+E+ 
Sbjct: 96  LNFLNRPTMQNVDYLTYGSADFFSAPYGLHWKFMKRICMVELFSSRALDSFVSVRSEELK 155

Query: 168 KLVERIEANSREKKPTDLRLESTTLTNNIISRMVF--STRCCGGKDEAQEIKDLAWRINM 227
           KL+ R+   +  ++  +L  +   LT+NII+RM+F        G ++++E+  +   +N 
Sbjct: 156 KLLIRVLKKAEAEESVNLGEQLKELTSNIITRMMFRKMQSDSDGGEKSEEVIKMVVELNE 215

Query: 228 LAGKLSLGDILGPLKVFDFSGNGKKFVKTLKKFDGLVERIMKEHEAANNGA--------- 287
           LAG  ++ +    LK  D  G  K+      K+D ++ERIM+EHE++   A         
Sbjct: 216 LAGFFNVSETFWFLKRLDLQGLKKRLKNARDKYDVIIERIMEEHESSKKNATGERNMLDV 275

Query: 288 -------------------------------------VLWAMGELLNRPESIQKLRKEII 347
                                                V WA+ EL+N PE ++K ++EI 
Sbjct: 276 LLDIYEDKNAEMKLTRENIKAFIMNIYGGGTDTSAITVEWALAELINHPEIMKKAQQEIE 335

Query: 348 SVVGNKKQVQESDLPNLPYLRAVVNETLRLHPSAPIIIRECLDDCYINGSLIKAKTRVLV 407
            VVGNK+ V+ESDL NL Y +AVV ET+RLHP  PI +RE  ++C + G  I AKTRV+V
Sbjct: 336 QVVGNKRVVEESDLCNLSYTQAVVKETMRLHPGGPIFVRESDEECAVAGFRIPAKTRVIV 395

Query: 408 NAYAVMRDPESWSEPDRFLPERFLEGSHENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSL 466
           N +A+ RD   W +P  F PERF EGS         E K  + + + FG+G+R CPG  +
Sbjct: 396 NVWAIGRDSNQWEDPLEFRPERF-EGS---------EWKVMSEKMMSFGAGRRSCPGEKM 455

[Close]

BLAST of Lsi03G017140 vs. TAIR 10
Match: AT3G20940.1 (cytochrome P450, family 705, subfamily A, polypeptide 30 )

HSP 1 Score: 286.6 bits (732), Expect = 3.7e-77
Identity = 184/498 (36.95%), Postives = 256/498 (51.41%), Query Frame = 0


Query: 17  FWFIIALLLHLL-LKKHLSNPKPPSPGTLAPVPSPPALPLIGHLHLLTPVLV-TSFQTLA 76
           F FI+  L  LL        PK    G   P PSPP+ P+IGHLHLL   LV  SFQ ++
Sbjct: 13  FIFILFFLFSLLCYSLFFRKPKGSRAGRDLP-PSPPSFPVIGHLHLLLSALVHKSFQNIS 72

Query: 77  CRYGPLIELRLGASKCVVVSTAAVAKDILKTHEHNFLSRPEFGASEYFIYRGSRFVMAQY 136
            +YGPL+ LR+     V+ S+A+VA +I K  + N  SR      E   +  S F  A Y
Sbjct: 73  SKYGPLLHLRVFHIPIVLASSASVAYEIFKAQDVNVSSRGHAPVGESLWFGSSSFFFAPY 132

Query: 137 GPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLVERIEANSREKKPTDLRLESTTLTN 196
           G Y++FM+KL  T+LL P  L  S  IR+DE+ +  + +   + +K+  ++  E+  L N
Sbjct: 133 GDYFKFMRKLIATKLLGPQALERSRKIRADELDRFYKTLLDKAMKKESVEIGEEAAKLNN 192

Query: 197 NIISRMVFSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDIL-GPLKVFDFSGNGKKFVK 256
           NII +M+    C     EA++ + L      L  ++  G I   PLK    S   K  + 
Sbjct: 193 NIICKMIMGRSCSEENGEAEKFRHLVIESMALTKQIFFGMIFHKPLKKLGISLFQKDILS 252

Query: 257 TLKKFDGLVERIMKEHE-------AANN-------------------------------- 316
             +KFD L+E+I+ EHE        AN+                                
Sbjct: 253 LSRKFDELLEKILFEHEEKKAEHNQANDMMDFLLEAYGDENAEYKITRNHIKSLFVDLVI 312

Query: 317 -------GAVLWAMGELLNRPESIQKLRKEIISVVGNKKQVQESDLPNLPYLRAVVNETL 376
                   A  W MGEL+N P+ +Q+LR+EI SVVGN + +QE+DLPNLPYL+AVV E L
Sbjct: 313 AGTDTSVQATQWTMGELINNPKILQRLREEIESVVGNTRLIQENDLPNLPYLQAVVKEGL 372

Query: 377 RLHPSAPIIIRECLDDCYINGSLIKAKTRVLVNAYAVMRDPESWSEPDRFLPERFLEGSH 436
           RLHP   I +R   + C + G  I  KT ++VN YA+MRDP  W +P+ F PERF+  S 
Sbjct: 373 RLHPPGSISVRMFQERCELKGFYIPEKTLLVVNTYAIMRDPNFWEDPEEFKPERFIASSR 432

Query: 437 ENIGGHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIATMVQRFDWKTGGNDSGNI 466
                   E++ +  +YIPF +G+RGCPGS+LA +     I  MVQ FDW+  G +  N+
Sbjct: 433 SE---QEDEVREEVLKYIPFSAGRRGCPGSNLAYISLGIVIGVMVQCFDWRIEG-EKVNM 492

The following BLAST results are available for this feature:

BLAST of Lsi03G017140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42798	3.6e-85	37.76	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
Q9XGT9	5.4e-81	36.38	Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1	[more]
O81973	1.6e-80	37.74	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]
Q9XHC6	3.5e-80	34.47	Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1	[more]
Q42799	1.3e-79	36.60	Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1	[more]

BLAST of Lsi03G017140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B805	1.5e-235	81.04	LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo O...	[more]
A0A5D3DP65	2.0e-235	80.89	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1J5T8	8.6e-223	76.67	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F1K7	3.0e-220	76.39	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0LBD2	1.0e-175	76.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1	[more]

Pages

BLAST of Lsi03G017140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905529.1	2.2e-236	80.69	3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida]	[more]
XP_008443083.1	3.1e-235	81.04	PREDICTED: LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase [Cucum...	[more]
KAA0043713.1	4.1e-235	80.89	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1...	[more]
XP_004136741.1	2.0e-226	78.50	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis sativus] >KAE8651142.1 hypoth...	[more]
XP_022982904.1	1.8e-222	76.67	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima]	[more]

BLAST of Lsi03G017140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42250.1	1.9e-145	55.96	cytochrome P450, family 712, subfamily A, polypeptide 1	[more]
AT5G06905.1	2.0e-86	36.71	cytochrome P450, family 712, subfamily A, polypeptide 2	[more]
AT2G14100.1	1.7e-82	38.49	cytochrome P450, family 705, subfamily A, polypeptide 13	[more]
AT5G06900.1	3.4e-78	36.05	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT3G20940.1	3.7e-77	36.95	cytochrome P450, family 705, subfamily A, polypeptide 30	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 405..414 score: 59.07 coord: 321..332 score: 47.67 coord: 286..299 score: 33.88 coord: 414..425 score: 34.16
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 277..448 e-value: 2.2E-51 score: 175.0 coord: 48..273 e-value: 2.3E-22 score: 79.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 360..384 score: 39.92 coord: 74..93 score: 34.11 coord: 192..210 score: 25.9 coord: 414..437 score: 28.7 coord: 320..338 score: 33.54 coord: 98..119 score: 22.73 coord: 404..414 score: 55.79
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 276..452 e-value: 1.9E-57 score: 197.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 43..275 e-value: 1.2E-36 score: 128.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 44..465
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 5..273
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 275..467
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 275..467
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 5..273
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 407..416

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G017140.1	Lsi03G017140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen