Lsi03G017110 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G017110
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionOTU domain-containing protein
Locationchr03: 28481307 .. 28485235 (-)
RNA-Seq ExpressionLsi03G017110
SyntenyLsi03G017110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGGTTTCCTTCTTTCTTTCTTTCTTTCACGTGCTTTTGGGTTTTTCTTCGAATTTTATATCTCCTTCCATTGTTTCTCCATTCTTTGATTTGATCGTTTTTTTGGCTTCAGTTCATCTTTCCCCGTTTGATTTCGTTTGTTAAATTAGTTTTTGTGATTCGAGTTGGTTCTTAGTTCTTTCATAAGCTTTGTGTTCAGAGTTTGCTGATTCTTGAGTTTGCCATTTTTCTTTAGTTTATGGTAATGGGAACTCTTATTATCTTCGTTGTTGTTGTTTTTCTTTCTCACCTTTCATTCTTTGTTGTTAATTCTATTTTGGGTATGTGCATTTTTTTAAAAAAAAGGAATAAATGGAGCTGAACTTTAATTCTACGAGCTGTGGTAGGTTGAAATTGAAGTTATATTCGATCGGGTTGTTTATTATAATTTTGATTTCAATGGATTATGAGATTATTTAGTATATTGATCAAATCGTTATTAAATGTAATACTGGAACGACTTAAATTATTATTTTCAACAATGAAAATTTAAGGGGAAACAATGTGTGATATGGAACACGCTGTGTATTCCACAGATACTATTAACTGTACTGTAAGAAGATTTGGGTTGTGCTACACATTTACAACATCTGAATTTTCAGCTTAATTGGACTATGAAGCCTAACCCTCTCTTTGAAATTCATGACATGGTAGGTTGTTATTTTTTTCTGAATCACGCTATTGTTAAACAGCTTTCTGTTTTTGGGAAATTTCTGAAATTTAGGTCCTCGATTAATATTTATTTAGGCACCGTTTGGTAATCATTTCATTTTTTTTTGTTTTTGAAAATTAAGCCTATTTCTTTTTATTTTCTTACCATGGTTTGTATCAATCTTAAGAAGAAGAGTTGCATTCTTAGCCAAATTCCAAAAACAAAAACTACTTTTTTTAGTTTTCAAATTTTGACTTGGTTTTTGAAAACATGGGTAAAAAGTAGATATCAAAGCAAGAGATTTAGAGGTGGAAGAAGTGTTTATAGGCGTAATTTTCATAAACCAAAAACTAAAAATGAAATTGTTACCAATTGGGGCTTAATTTTTTGTTTTTGTTTCTGGGTTTTAAAAATTAAGCTTATAAACTGTAATTCTACCCATAAGTTTCAATGTTTTGTTATCTACTTTCTACCCATATTTTCAAAAACCAAGTAAAATATTGAAACTAAAAAAAAGTAGTTTCCAAAAACTTGTTTTTGTTTTTAGAGTTTAGTTAGAGTTCAAGTGTCCTTGAGAAATGTGAGAACCATTATTGAAACAGGAAACAAGATATAAATTTCAAAAACAAAAAATAAAACATGAAGTCATTATCAAATGGAGTCTAAGTTTACACTTCCAGTTTTTTTTTTTTTTTTTTCGATCACGAGATTGTCTTTGTATCTCCCAACCACTTCTATGCTGTTCCCAGTCTTCTTAGAGAGTATGATTCTCATTTCTAGAATTAAATGAGGTACCAGTAGGGTAAGAGAACCTAGTAGCCAAGGTAGAGCCTTCCTCTTCTTCCTATTTTTTGGCCCATCATTAGCCTCTTCAGATTTCCTTATTTTTAAATGCTTCTCTAATCATTATACTACCTTGTTGAGACCAAGTAAGAACTTCAATACCTGCTCCTTCTCCGCTAGTTTAGTATATACTTAACTCTTATAGCTAACATTTTCCTCATGTATCTCATTCATTAGATTTCTTATCCCATCTAAGGAAAGAAATCCATACAACTTAGAATATTCAAAGGATTTCTATTAACTCCTCTTGTTATTATATATAATGCAATAATATACAAGCTATAACAAATTGACGATGGAATGTTATGAACGACTAGTAGTTTCAAAGTGGCTGAGAATATAAATGTTGGATGATATAAGATAGTTGCATTAGTTATTTGTTGAGAAAGATCACTTTGGCATGGTCTGCATGGACGGTCACCTGTTTTGTTATTTTCTTTGTCCCCCTTTCTGTTTAATAACGTTTGTAATTGTACCAATGTAATGCTTTCGATTGCTAATTTGTTCTGTTAATTCCTCAGGCTGCACCACCATGAGTATCGGCTCAATCAGTGTTTGCACAAGGAATGCTGTCCCTCTGAACTTCCGTGTTTGCACACAGATGGGCAGCAACATCTGTACAGTGCTGTCCCGAAGAACATCAACTTCGTGCTGTTCCTATGGCATTTCAAGACCAAAGTATGCTGATCAATCCGTCACCACAATATCGTCTTGTTCACCAAATACTAGCCAAAGATATCAGGGAGGTTGTTTCAGTTCTTGCTTCTCTAGAAGGTCAGTTGACTTCAAAGCTTTAACGGTTAAAGATCTGATCACCGATGGAGGATCACGTGGACGAGAACTTGAAATTTCAATGGCATGTAAAGGTATGAATGTGAAGCTCTCAATCCCCAATGATGGGATGTTTAGCAAAATCAAGTGTAACATGCGATGGCCAGAAAGATGGGCATCTACTGGCTTAGTTTTTGGGTGGGTGGTTTGTTATTCAACTTCTGAACCAGTCCATGCTGAAGCAGCTTATGAGAAAGAGGATAATGAAGAAAACTCTGATTCATCTCAAGTCAAATTCTCTCATGGAAAGAAGGTTTACACCGACTATTCTGTTATCGGTGAGCTTCAAAGTCTTCCATTTCCTTGTTGTACGTTAAATGGTCAATTGATAATTGCTTGTACAACCTGTTATCAAAATACCATGGATTAAAGGGGAAAATGTTAATGTAAGCAACCTGTTTGTTCTGGACAGGAATTCCTGGAGATGGAAGATGTTTGTTTCGCTCAGTCGCTCATGGTGCTTGTTTGCGATCTGGGAAACCAGCTCCAAGTGAGAGCCTTCAGAGAGAGTTGGCAGATGATTTGCGTTCAAAAGTACGTTTTATTAGTTTGTTTTAAAGAGAATACCATGGTTGCTTAACTTACACTTACACACACCCTACTGTAGCTATCTAGATTCCATCTTTCGGGCCGATTTCTTCGCAAACTTGTATATGGATGCCCCTTTACTTAAGAACGGTGCATCACACATCTTTCAGGTTGCAGATGAGTTTATCAAGAGACGCGAAGAAACAGAATGGTAAATCTCTCTCTCACAAAAGTTTCACATCAATGACTTTACTCTTGTAGGATCATAAAACATGAACACTTCCTGAAATTGGGTTTTCCTGCTTGTGTTGTCAACAGGTTTGTTGAAGGTGATTTCGATATGTATGTGTCAAATATGCGAAAACCACACGTGTGGGGAGGCGAGCCGGAGTTATTCATGGCTTCACATGTTCTTCAGTAAGCTCCCTCTCAGTCTTCTCTGTGTGTTGTGTACTGCATATATCAGTGAATTGTTTATGATGAAGGCTGTGATTGCTGCTATGCAGTGCACCTATCATAGTGTACATGTACGATAAAGATTCTGGTGGGTTGATTTCCATAGCTGAATATGGCGATGAATATGGCAAAGAAAATCCAATCAGAGTTCTGTACCATGGTTTTGGCCATTACGATGCTTTGCAAATTCCTGCAAATCAAGGGGTAGGTAGATCTAAACTCTAGTGTTCTAACTGAATCCATTGTGATTTCAATTACATTCTCTTTCATTCTATAAAATAAATCTCTTTCCATTCCAATGAGAATTCAGCATGGAGACTTCACTCTATATTTAATGCTAATGGAAGATAAGTAGAGAATCTCTCTCAAATTTCTAAAAGTTAAAGTAGATTTTTGGCTTTTACTTCCACCTATGAATTTATTTGTAATCAATATTATTATCTCAAATGTTGTTCGATCTACACTCTCATAATGGTTAAACTTTAAAAAACACACACACACAATGATTAATGATTAATCAAATATCAATTGAATCATAATTGGATTTGGTTTTTTTTTTTTAATCAAAGAAAAAACATT

mRNA sequence

TTGGGTTTCCTTCTTTCTTTCTTTCTTTCACGTGCTTTTGGGTTTTTCTTCGAATTTTATATCTCCTTCCATTGTTTCTCCATTCTTTGATTTGATCGTTTTTTTGGCTTCAGTTCATCTTTCCCCGTTTGATTTCGTTTGTTAAATTAGTTTTTGTGATTCGAGTTGGTTCTTAGTTCTTTCATAAGCTTTGTGTTCAGAGTTTGCTGATTCTTGAGTTTGCCATTTTTCTTTAGTTTATGATACTATTAACTGTACTGTAAGAAGATTTGGGTTGTGCTACACATTTACAACATCTGAATTTTCAGCTTAATTGGACTATGAAGCCTAACCCTCTCTTTGAAATTCATGACATGGCTGCACCACCATGAGTATCGGCTCAATCAGTGTTTGCACAAGGAATGCTGTCCCTCTGAACTTCCGTGTTTGCACACAGATGGGCAGCAACATCTGTACAGTGCTGTCCCGAAGAACATCAACTTCGTGCTGTTCCTATGGCATTTCAAGACCAAAGTATGCTGATCAATCCGTCACCACAATATCGTCTTGTTCACCAAATACTAGCCAAAGATATCAGGGAGGTTGTTTCAGTTCTTGCTTCTCTAGAAGGTCAGTTGACTTCAAAGCTTTAACGGTTAAAGATCTGATCACCGATGGAGGATCACGTGGACGAGAACTTGAAATTTCAATGGCATGTAAAGGTATGAATGTGAAGCTCTCAATCCCCAATGATGGGATGTTTAGCAAAATCAAGTGTAACATGCGATGGCCAGAAAGATGGGCATCTACTGGCTTAGTTTTTGGGTGGGTGGTTTGTTATTCAACTTCTGAACCAGTCCATGCTGAAGCAGCTTATGAGAAAGAGGATAATGAAGAAAACTCTGATTCATCTCAAGTCAAATTCTCTCATGGAAAGAAGGTTTACACCGACTATTCTGTTATCGGAATTCCTGGAGATGGAAGATGTTTGTTTCGCTCAGTCGCTCATGGTGCTTGTTTGCGATCTGGGAAACCAGCTCCAAGTGAGAGCCTTCAGAGAGAGTTGGCAGATGATTTGCGTTCAAAAGTTGCAGATGAGTTTATCAAGAGACGCGAAGAAACAGAATGGTTTGTTGAAGGTGATTTCGATATGTATGTGTCAAATATGCGAAAACCACACGTGTGGGGAGGCGAGCCGGAGTTATTCATGGCTTCACATGTTCTTCATGCACCTATCATAGTGTACATGTACGATAAAGATTCTGGTGGGTTGATTTCCATAGCTGAATATGGCGATGAATATGGCAAAGAAAATCCAATCAGAGTTCTGTACCATGGTTTTGGCCATTACGATGCTTTGCAAATTCCTGCAAATCAAGGGGTAGGTAGATCTAAACTCTAGTGTTCTAACTGAATCCATTGTGATTTCAATTACATTCTCTTTCATTCTATAAAATAAATCTCTTTCCATTCCAATGAGAATTCAGCATGGAGACTTCACTCTATATTTAATGCTAATGGAAGATAAGTAGAGAATCTCTCTCAAATTTCTAAAAGTTAAAGTAGATTTTTGGCTTTTACTTCCACCTATGAATTTATTTGTAATCAATATTATTATCTCAAATGTTGTTCGATCTACACTCTCATAATGGTTAAACTTTAAAAAACACACACACACAATGATTAATGATTAATCAAATATCAATTGAATCATAATTGGATTTGGTTTTTTTTTTTTAATCAAAGAAAAAACATT

Coding sequence (CDS)

ATGAGTATCGGCTCAATCAGTGTTTGCACAAGGAATGCTGTCCCTCTGAACTTCCGTGTTTGCACACAGATGGGCAGCAACATCTGTACAGTGCTGTCCCGAAGAACATCAACTTCGTGCTGTTCCTATGGCATTTCAAGACCAAAGTATGCTGATCAATCCGTCACCACAATATCGTCTTGTTCACCAAATACTAGCCAAAGATATCAGGGAGGTTGTTTCAGTTCTTGCTTCTCTAGAAGGTCAGTTGACTTCAAAGCTTTAACGGTTAAAGATCTGATCACCGATGGAGGATCACGTGGACGAGAACTTGAAATTTCAATGGCATGTAAAGGTATGAATGTGAAGCTCTCAATCCCCAATGATGGGATGTTTAGCAAAATCAAGTGTAACATGCGATGGCCAGAAAGATGGGCATCTACTGGCTTAGTTTTTGGGTGGGTGGTTTGTTATTCAACTTCTGAACCAGTCCATGCTGAAGCAGCTTATGAGAAAGAGGATAATGAAGAAAACTCTGATTCATCTCAAGTCAAATTCTCTCATGGAAAGAAGGTTTACACCGACTATTCTGTTATCGGAATTCCTGGAGATGGAAGATGTTTGTTTCGCTCAGTCGCTCATGGTGCTTGTTTGCGATCTGGGAAACCAGCTCCAAGTGAGAGCCTTCAGAGAGAGTTGGCAGATGATTTGCGTTCAAAAGTTGCAGATGAGTTTATCAAGAGACGCGAAGAAACAGAATGGTTTGTTGAAGGTGATTTCGATATGTATGTGTCAAATATGCGAAAACCACACGTGTGGGGAGGCGAGCCGGAGTTATTCATGGCTTCACATGTTCTTCATGCACCTATCATAGTGTACATGTACGATAAAGATTCTGGTGGGTTGATTTCCATAGCTGAATATGGCGATGAATATGGCAAAGAAAATCCAATCAGAGTTCTGTACCATGGTTTTGGCCATTACGATGCTTTGCAAATTCCTGCAAATCAAGGGGTAGGTAGATCTAAACTCTAG

Protein sequence

MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISSCSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIPNDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL
Homology
BLAST of Lsi03G017110 vs. ExPASy Swiss-Prot
Match: Q8LBZ4 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=OTU4 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.2e-78
Identity = 173/340 (50.88%), Postives = 218/340 (64.12%), Query Frame = 0

Query: 6   ISVCTRNAVPLNFRVCTQMGSNICTVLSRRTST-SCCSY--GISRPKYADQSVTTISSCS 65
           I+ C+RNA+ +       +GS +  V++  +S  SC S   G+SR  Y    V    S S
Sbjct: 8   ITTCSRNAISIK----RHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIPND 125
              S  +  G F S    R       TV        +   +LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GMFSKIKCNMR---WPERWASTGLVFGWVVCY-STSEPVHAEAAYEKEDNEENSDSSQVK 185
              SKI+ NMR      R+A TG++   +VCY STS+  +A+++ +K+ N  +  SS  K
Sbjct: 128 ---SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGK 187

Query: 186 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 245
           F +GK+VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QRELAD+LR++VADEF
Sbjct: 188 FHNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEF 247

Query: 246 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 305
           I+RR+ETEWFVEGDFD YV  +R PHVWGGEPELFMASHVL  PI VYM D  +GGLISI
Sbjct: 248 IQRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISI 307

Query: 306 AEYGDEYGKENPIRVLYHGFGHYDALQI-PANQGVGRSKL 338
           AEYG EYGK++PIRVLYHGFGHYDAL +  +   + +SKL
Sbjct: 308 AEYGQEYGKDDPIRVLYHGFGHYDALLLHESKASIPKSKL 317

BLAST of Lsi03G017110 vs. ExPASy Swiss-Prot
Match: Q8GYW0 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=OTU3 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.5e-13
Identity = 55/175 (31.43%), Postives = 82/175 (46.86%), Query Frame = 0

Query: 174 SSQVKFSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSK 233
           +S +  S   K    Y+V  + GDGRCLFR++  G     G    +   +R+ AD+LR  
Sbjct: 61  NSSMNRSPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITL-NPQRERDDADELRMA 120

Query: 234 VADEFIKRREETEWF--------VEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIV 293
           V +      +E E +        V+     +   + +   WGGE EL + S +   PIIV
Sbjct: 121 VKEVICNDPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIV 180

Query: 294 YMYDKDSG-------GLISIAEYGDEY------GK--ENPIRVLYHGFGHYDALQ 326
           Y+ + + G       G I I EYG E+      GK  +N +R+LY G  HYD L+
Sbjct: 181 YIPEHEHGRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLLR 234

BLAST of Lsi03G017110 vs. ExPASy Swiss-Prot
Match: Q6GM06 (Deubiquitinase OTUD6B OS=Xenopus laevis OX=8355 GN=otud6b PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.8e-06
Identity = 43/183 (23.50%), Postives = 85/183 (46.45%), Query Frame = 0

Query: 160 EAAYEKEDNEENSDSSQVKFSHGKKVYT----------DYSVIGIPGDGRCLFRSVAHGA 219
           +AA EKE ++  +++     S  + + +          +  +  IP DG C++R++ H  
Sbjct: 111 KAAQEKERDDRIAEAEIANLSGARHLESQKLAQILAERELQIRQIPSDGHCMYRAIEH-Q 170

Query: 220 CLRSGKPAPSESLQRELADDLRSKVAD--EFIKRREETEWFVEGDFDMYVSNMRKPHVWG 279
               G      +L+ + AD ++ +  D   F+      + + + +F  Y +++     WG
Sbjct: 171 LNEQGNSLTVANLRSQTADYMQKRAEDFLPFLTNSSTGDMYTQEEFQKYCTDIVNTPAWG 230

Query: 280 GEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLY--HGFG---HYD 326
           G+ EL   SH+L  PI V   +      + I   G+EY  + PI ++Y  H +G   HY+
Sbjct: 231 GQLELRALSHILKTPIEVIQAES-----LPIV-IGEEYSNK-PITLVYMRHAYGLGEHYN 285

BLAST of Lsi03G017110 vs. ExPASy Swiss-Prot
Match: Q96G74 (OTU domain-containing protein 5 OS=Homo sapiens OX=9606 GN=OTUD5 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.0e-06
Identity = 40/184 (21.74%), Postives = 77/184 (41.85%), Query Frame = 0

Query: 151 YSTSEPVHAEAAYEKEDNEENSDSSQVKFSHGKKVYTDYSVIGIPGDGRCLFRSVAHGAC 210
           Y++ +   A AA  +  +    +  +  F    +    + +  +  DG CLFR+V     
Sbjct: 175 YNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAV----- 234

Query: 211 LRSGKPAPSESLQRELADDLRSKVADEFIKRREETEWFVEGDFDMYVSNMRKPHVWGGEP 270
                 A      +++ + +R    D  +K  +    +V  DF  Y++  RK +  G   
Sbjct: 235 ------ADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHI 294

Query: 271 ELFMASHVLHAPIIVYMYDKDSGGLISIAEY-GDEYGKENPIRVLYHGFGHYDALQIPAN 330
           E+   + + + P+ VY Y   +  +  I  + G    ++ PIRV YH   HY+++  P  
Sbjct: 295 EMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNK 347

Query: 331 QGVG 334
             +G
Sbjct: 355 ATIG 347

BLAST of Lsi03G017110 vs. ExPASy Swiss-Prot
Match: Q640H3 (OTU domain-containing protein 5-B OS=Xenopus laevis OX=8355 GN=otud5-b PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.0e-05
Identity = 34/137 (24.82%), Postives = 60/137 (43.80%), Query Frame = 0

Query: 197 DGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIKRREETEWFVEGDFDMY 256
           DG CLFR+V           A      +++ + +R    D  +K  +    +V  DF  Y
Sbjct: 179 DGACLFRAV-----------ADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTY 238

Query: 257 VSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYH 316
           ++  RK +  G   E+   + + + P+ VY Y  +         +G +  ++ PIRV YH
Sbjct: 239 INRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYGTEPINTF----HGIQQNEDEPIRVSYH 298

Query: 317 GFGHYDALQIPANQGVG 334
              HY+++  P    +G
Sbjct: 299 RNIHYNSVVNPNKATIG 300

BLAST of Lsi03G017110 vs. ExPASy TrEMBL
Match: A0A1S3B6T1 (OTU domain-containing protein At3g57810 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486791 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.1e-183
Identity = 313/337 (92.88%), Postives = 324/337 (96.14%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSIS+CTRNAVPLN R+ TQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           CSPNTSQR+QGGC S+CFSRRS+DF+A TVKDLITDGGS GRE+EIS+ACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFS 180
           NDG FSKIK NMRWPERWAS GLVFGWVVCYSTSEPVHAEAAYEK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIK 240
           HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQR+LAD+LRS VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RREETEWFVEGDFD YVSNMRKPHVWGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 338
           YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 337

BLAST of Lsi03G017110 vs. ExPASy TrEMBL
Match: A0A5D3DP86 (OTU domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005670 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.1e-183
Identity = 313/337 (92.88%), Postives = 324/337 (96.14%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSIS+CTRNAVPLN R+ TQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           CSPNTSQR+QGGC S+CFSRRS+DF+A TVKDLITDGGS GRE+EIS+ACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFS 180
           NDG FSKIK NMRWPERWAS GLVFGWVVCYSTSEPVHAEAAYEK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIK 240
           HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQR+LAD+LRS VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RREETEWFVEGDFD YVSNMRKPHVWGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 338
           YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 337

BLAST of Lsi03G017110 vs. ExPASy TrEMBL
Match: A0A6J1FB45 (OTU domain-containing protein At3g57810 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442443 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.0e-169
Identity = 295/340 (86.76%), Postives = 312/340 (91.76%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSISVCTRN +PLNFRVCTQMGSNICTVLS RT TSCCSYGISRPKY DQSVT ISS
Sbjct: 1   MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           C+PNT QR QGGC S CFSRR  DF+ALTVKDLITDGGSRGR LEIS+ACKGMN K+S+P
Sbjct: 61  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 120

Query: 121 NDGMFSKIKCNMRWPERW--ASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVK 180
           +DGM SKIK NMRWPER   AS GLVFGWVVC STSEPVHAEAAY+K+ NEEN+DSS VK
Sbjct: 121 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 180

Query: 181 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 240
           FSHGKKVY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQRELAD+LR+KVADEF
Sbjct: 181 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 240

Query: 241 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRREETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 241 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 300

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPANQG-VGRSKL 338
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPANQG  GRSKL
Sbjct: 301 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQAGRSKL 340

BLAST of Lsi03G017110 vs. ExPASy TrEMBL
Match: A0A6J1F5B0 (OTU domain-containing protein At3g57810 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442443 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.0e-169
Identity = 295/340 (86.76%), Postives = 312/340 (91.76%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSISVCTRN +PLNFRVCTQMGSNICTVLS RT TSCCSYGISRPKY DQSVT ISS
Sbjct: 13  MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 72

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           C+PNT QR QGGC S CFSRR  DF+ALTVKDLITDGGSRGR LEIS+ACKGMN K+S+P
Sbjct: 73  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 132

Query: 121 NDGMFSKIKCNMRWPERW--ASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVK 180
           +DGM SKIK NMRWPER   AS GLVFGWVVC STSEPVHAEAAY+K+ NEEN+DSS VK
Sbjct: 133 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 192

Query: 181 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 240
           FSHGKKVY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQRELAD+LR+KVADEF
Sbjct: 193 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 252

Query: 241 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRREETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 253 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 312

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPANQG-VGRSKL 338
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPANQG  GRSKL
Sbjct: 313 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQAGRSKL 352

BLAST of Lsi03G017110 vs. ExPASy TrEMBL
Match: A0A6J1DJP8 (OTU domain-containing protein At3g57810 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021529 PE=4 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 295/339 (87.02%), Postives = 310/339 (91.45%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRN V LN RV  QMGSNIC+VLS R S SCCSYGISRPKYADQSVTTISS
Sbjct: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSPRISNSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           C+PNTSQR+ GGC S CFSR+ +DFK LTVKDLITDGGS GR LEIS+ACKGMNVKLSI 
Sbjct: 61  CTPNTSQRFPGGCLSYCFSRQPIDFKNLTVKDLITDGGSHGRGLEISLACKGMNVKLSIT 120

Query: 121 NDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFS 180
           NDG FSKIK NMRWPER AS GLVFGWVVCYS+SEPVHAEAA EKEDNEENSDSS VKFS
Sbjct: 121 NDG-FSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFS 180

Query: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIK 240
           HGK VYTDYSVIGIPGDGRCLFRSVAHGACLRSGK APSESLQR+LAD+LR+KVADEFIK
Sbjct: 181 HGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKAAPSESLQRDLADELRNKVADEFIK 240

Query: 241 RREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RR+ETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE
Sbjct: 241 RRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIP--ANQGVGRSKL 338
           YGDEYGKENPIRVLYHGFGHYDALQIP  A++GV RSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 338

BLAST of Lsi03G017110 vs. NCBI nr
Match: XP_008443087.1 (PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443088.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443089.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443090.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >KAA0043710.1 OTU domain-containing protein [Cucumis melo var. makuwa] >TYK25424.1 OTU domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 651.4 bits (1679), Expect = 4.4e-183
Identity = 313/337 (92.88%), Postives = 324/337 (96.14%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSIS+CTRNAVPLN R+ TQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           CSPNTSQR+QGGC S+CFSRRS+DF+A TVKDLITDGGS GRE+EIS+ACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFS 180
           NDG FSKIK NMRWPERWAS GLVFGWVVCYSTSEPVHAEAAYEK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIK 240
           HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQR+LAD+LRS VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RREETEWFVEGDFD YVSNMRKPHVWGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 338
           YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 337

BLAST of Lsi03G017110 vs. NCBI nr
Match: XP_011652109.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus] >XP_011652110.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus] >XP_011652111.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 640.6 bits (1651), Expect = 7.7e-180
Identity = 311/338 (92.01%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSIS+CTRNAVPLN R  TQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLR--TQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQ-GGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSI 120
           CSPNTSQR+Q GGC S+CFSRRS+DF+A TVKDLITDGGSRGR++EIS+ACKGMNVKLSI
Sbjct: 61  CSPNTSQRFQAGGCLSTCFSRRSIDFQAFTVKDLITDGGSRGRDVEISLACKGMNVKLSI 120

Query: 121 PNDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKF 180
           PNDG FSKIK NMRWPERWAS GLVFGWVVCYSTSEPVHAEAAYEK+DNEENSDSS VK 
Sbjct: 121 PNDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKL 180

Query: 181 SHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFI 240
           SHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQR+LAD+LRS VADEFI
Sbjct: 181 SHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFI 240

Query: 241 KRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIA 300
           KRREETEWFVEGDFD Y+SNMR PHVWGGEPELFMASHVL APIIVYMYDKDSGGLISIA
Sbjct: 241 KRREETEWFVEGDFDTYMSNMRNPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIA 300

Query: 301 EYGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 338
           EYGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL
Sbjct: 301 EYGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 336

BLAST of Lsi03G017110 vs. NCBI nr
Match: XP_038903218.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Benincasa hispida] >XP_038903219.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Benincasa hispida] >XP_038903220.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 630.2 bits (1624), Expect = 1.0e-176
Identity = 307/337 (91.10%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSISV TRNAVPLNFRVCTQMGSNICTVLSR TSTSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISVSTRNAVPLNFRVCTQMGSNICTVLSRGTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           CSP +SQR+QG C  SCFSRRS+DF+ALTVKDLI+DGGS GRELEIS+A KGMN+KLSIP
Sbjct: 61  CSPKSSQRFQGRCLISCFSRRSIDFRALTVKDLISDGGSCGRELEISLALKGMNMKLSIP 120

Query: 121 NDGMFSKIKCNMRWPERWASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVKFS 180
           ND M SKIKCN RWPERWAS GLVFGWVVCYSTSEPVHAEAAYE++D EENS SS VKFS
Sbjct: 121 NDVMSSKIKCNRRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEEDDAEENSGSSHVKFS 180

Query: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIK 240
           HGKKVYT+YSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQR LAD+LRSKVADEFIK
Sbjct: 181 HGKKVYTNYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRVLADELRSKVADEFIK 240

Query: 241 RREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RREETEWFVEG+FD YVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGNFDTYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPANQGVGRSKL 338
           YGDEYGKENPIRVLYHGFGHYDALQIPA QGVGRSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIPAKQGVGRSKL 337

BLAST of Lsi03G017110 vs. NCBI nr
Match: XP_022935652.1 (OTU domain-containing protein At3g57810 isoform X2 [Cucurbita moschata] >XP_022935653.1 OTU domain-containing protein At3g57810 isoform X2 [Cucurbita moschata])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 295/340 (86.76%), Postives = 312/340 (91.76%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSISVCTRN +PLNFRVCTQMGSNICTVLS RT TSCCSYGISRPKY DQSVT ISS
Sbjct: 1   MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 60

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           C+PNT QR QGGC S CFSRR  DF+ALTVKDLITDGGSRGR LEIS+ACKGMN K+S+P
Sbjct: 61  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 120

Query: 121 NDGMFSKIKCNMRWPERW--ASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVK 180
           +DGM SKIK NMRWPER   AS GLVFGWVVC STSEPVHAEAAY+K+ NEEN+DSS VK
Sbjct: 121 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 180

Query: 181 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 240
           FSHGKKVY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQRELAD+LR+KVADEF
Sbjct: 181 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 240

Query: 241 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRREETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 241 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 300

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPANQG-VGRSKL 338
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPANQG  GRSKL
Sbjct: 301 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQAGRSKL 340

BLAST of Lsi03G017110 vs. NCBI nr
Match: XP_022935651.1 (OTU domain-containing protein At3g57810 isoform X1 [Cucurbita moschata])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 295/340 (86.76%), Postives = 312/340 (91.76%), Query Frame = 0

Query: 1   MSIGSISVCTRNAVPLNFRVCTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60
           MSIGSISVCTRN +PLNFRVCTQMGSNICTVLS RT TSCCSYGISRPKY DQSVT ISS
Sbjct: 13  MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 72

Query: 61  CSPNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIP 120
           C+PNT QR QGGC S CFSRR  DF+ALTVKDLITDGGSRGR LEIS+ACKGMN K+S+P
Sbjct: 73  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 132

Query: 121 NDGMFSKIKCNMRWPERW--ASTGLVFGWVVCYSTSEPVHAEAAYEKEDNEENSDSSQVK 180
           +DGM SKIK NMRWPER   AS GLVFGWVVC STSEPVHAEAAY+K+ NEEN+DSS VK
Sbjct: 133 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 192

Query: 181 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 240
           FSHGKKVY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQRELAD+LR+KVADEF
Sbjct: 193 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 252

Query: 241 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRREETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 253 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 312

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPANQG-VGRSKL 338
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPANQG  GRSKL
Sbjct: 313 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQAGRSKL 352

BLAST of Lsi03G017110 vs. TAIR 10
Match: AT3G57810.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.7e-79
Identity = 173/340 (50.88%), Postives = 218/340 (64.12%), Query Frame = 0

Query: 6   ISVCTRNAVPLNFRVCTQMGSNICTVLSRRTST-SCCSY--GISRPKYADQSVTTISSCS 65
           I+ C+RNA+ +       +GS +  V++  +S  SC S   G+SR  Y    V    S S
Sbjct: 8   ITTCSRNAISIK----RHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIPND 125
              S  +  G F S    R       TV        +   +LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GMFSKIKCNMR---WPERWASTGLVFGWVVCY-STSEPVHAEAAYEKEDNEENSDSSQVK 185
              SKI+ NMR      R+A TG++   +VCY STS+  +A+++ +K+ N  +  SS  K
Sbjct: 128 ---SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGK 187

Query: 186 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 245
           F +GK+VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QRELAD+LR++VADEF
Sbjct: 188 FHNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEF 247

Query: 246 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 305
           I+RR+ETEWFVEGDFD YV  +R PHVWGGEPELFMASHVL  PI VYM D  +GGLISI
Sbjct: 248 IQRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISI 307

Query: 306 AEYGDEYGKENPIRVLYHGFGHYDALQI-PANQGVGRSKL 338
           AEYG EYGK++PIRVLYHGFGHYDAL +  +   + +SKL
Sbjct: 308 AEYGQEYGKDDPIRVLYHGFGHYDALLLHESKASIPKSKL 317

BLAST of Lsi03G017110 vs. TAIR 10
Match: AT3G57810.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.7e-79
Identity = 173/340 (50.88%), Postives = 218/340 (64.12%), Query Frame = 0

Query: 6   ISVCTRNAVPLNFRVCTQMGSNICTVLSRRTST-SCCSY--GISRPKYADQSVTTISSCS 65
           I+ C+RNA+ +       +GS +  V++  +S  SC S   G+SR  Y    V    S S
Sbjct: 8   ITTCSRNAISIK----RHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRYQGGCFSSCFSRRSVDFKALTVKDLITDGGSRGRELEISMACKGMNVKLSIPND 125
              S  +  G F S    R       TV        +   +LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GMFSKIKCNMR---WPERWASTGLVFGWVVCY-STSEPVHAEAAYEKEDNEENSDSSQVK 185
              SKI+ NMR      R+A TG++   +VCY STS+  +A+++ +K+ N  +  SS  K
Sbjct: 128 ---SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGK 187

Query: 186 FSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEF 245
           F +GK+VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QRELAD+LR++VADEF
Sbjct: 188 FHNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEF 247

Query: 246 IKRREETEWFVEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 305
           I+RR+ETEWFVEGDFD YV  +R PHVWGGEPELFMASHVL  PI VYM D  +GGLISI
Sbjct: 248 IQRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISI 307

Query: 306 AEYGDEYGKENPIRVLYHGFGHYDALQI-PANQGVGRSKL 338
           AEYG EYGK++PIRVLYHGFGHYDAL +  +   + +SKL
Sbjct: 308 AEYGQEYGKDDPIRVLYHGFGHYDALLLHESKASIPKSKL 317

BLAST of Lsi03G017110 vs. TAIR 10
Match: AT3G57810.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.4e-75
Identity = 135/202 (66.83%), Postives = 164/202 (81.19%), Query Frame = 0

Query: 138 WASTGLVFGWVVCY-STSEPVHAEAAYEKEDNEENSDSSQVKFSHGKKVYTDYSVIGIPG 197
           +A TG++   +VCY STS+  +A+++ +K+ N  +  SS  KF +GK+VYTDYS+IGIPG
Sbjct: 73  FAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGKFHNGKRVYTDYSIIGIPG 132

Query: 198 DGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSKVADEFIKRREETEWFVEGDFDMY 257
           DGRCLFRSVAHG CLRSGK AP E +QRELAD+LR++VADEFI+RR+ETEWFVEGDFD Y
Sbjct: 133 DGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEFIQRRQETEWFVEGDFDTY 192

Query: 258 VSNMRKPHVWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYH 317
           V  +R PHVWGGEPELFMASHVL  PI VYM D  +GGLISIAEYG EYGK++PIRVLYH
Sbjct: 193 VRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISIAEYGQEYGKDDPIRVLYH 252

Query: 318 GFGHYDALQI-PANQGVGRSKL 338
           GFGHYDAL +  +   + +SKL
Sbjct: 253 GFGHYDALLLHESKASIPKSKL 274

BLAST of Lsi03G017110 vs. TAIR 10
Match: AT2G38025.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-14
Identity = 55/175 (31.43%), Postives = 82/175 (46.86%), Query Frame = 0

Query: 174 SSQVKFSHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRELADDLRSK 233
           +S +  S   K    Y+V  + GDGRCLFR++  G     G    +   +R+ AD+LR  
Sbjct: 61  NSSMNRSPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITL-NPQRERDDADELRMA 120

Query: 234 VADEFIKRREETEWF--------VEGDFDMYVSNMRKPHVWGGEPELFMASHVLHAPIIV 293
           V +      +E E +        V+     +   + +   WGGE EL + S +   PIIV
Sbjct: 121 VKEVICNDPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIV 180

Query: 294 YMYDKDSG-------GLISIAEYGDEY------GK--ENPIRVLYHGFGHYDALQ 326
           Y+ + + G       G I I EYG E+      GK  +N +R+LY G  HYD L+
Sbjct: 181 YIPEHEHGRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLLR 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBZ45.2e-7850.88OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thalian... [more]
Q8GYW01.5e-1331.43OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thalian... [more]
Q6GM061.8e-0623.50Deubiquitinase OTUD6B OS=Xenopus laevis OX=8355 GN=otud6b PE=2 SV=1[more]
Q96G749.0e-0621.74OTU domain-containing protein 5 OS=Homo sapiens OX=9606 GN=OTUD5 PE=1 SV=1[more]
Q640H32.0e-0524.82OTU domain-containing protein 5-B OS=Xenopus laevis OX=8355 GN=otud5-b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3B6T12.1e-18392.88OTU domain-containing protein At3g57810 isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5D3DP862.1e-18392.88OTU domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1FB453.0e-16986.76OTU domain-containing protein At3g57810 isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1F5B03.0e-16986.76OTU domain-containing protein At3g57810 isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1DJP81.8e-16687.02OTU domain-containing protein At3g57810 isoform X1 OS=Momordica charantia OX=367... [more]
Match NameE-valueIdentityDescription
XP_008443087.14.4e-18392.88PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP... [more]
XP_011652109.17.7e-18092.01OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sa... [more]
XP_038903218.11.0e-17691.10OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Benincasa ... [more]
XP_022935652.16.1e-16986.76OTU domain-containing protein At3g57810 isoform X2 [Cucurbita moschata] >XP_0229... [more]
XP_022935651.16.1e-16986.76OTU domain-containing protein At3g57810 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G57810.23.7e-7950.88Cysteine proteinases superfamily protein [more]
AT3G57810.33.7e-7950.88Cysteine proteinases superfamily protein [more]
AT3G57810.11.4e-7566.83Cysteine proteinases superfamily protein [more]
AT2G38025.11.1e-1431.43Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003323OTU domainPFAMPF02338OTUcoord: 195..321
e-value: 1.8E-27
score: 96.3
IPR003323OTU domainPROSITEPS50802OTUcoord: 189..327
score: 15.532407
NoneNo IPR availableGENE3D3.90.70.80coord: 162..335
e-value: 1.4E-36
score: 127.6
NoneNo IPR availablePANTHERPTHR12419OTU DOMAIN CONTAINING PROTEINcoord: 8..336
NoneNo IPR availablePANTHERPTHR12419:SF8OS09G0487700 PROTEINcoord: 8..336
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 289..301
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 188..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G017110.1Lsi03G017110.1mRNA