Lsi03G016700 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G016700 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.8e-58
Identity = 188/651 (28.88%), Postives = 307/651 (47.16%), Query Frame = 0

Query: 53  SLKAILMDA-EKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKAYKQKAIGKQVFVSSLS 112
           +++A+L DA EK+     + +WL++L +   +++  + E K +A + +      +   + 
Sbjct: 40  TIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIRFEQSRLGFYHPGII 99

Query: 113 SSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMKPSWQLLYPLT 172
           + + +  ++MK++ + LD I+ +  +F+     T     +AT       +  + L  P  
Sbjct: 100 NFRHKIGRRMKEIMEKLDAISEERRKFHFLEKITERQAAAATR------ETGFVLTEPKV 159

Query: 173 NARQVFHGSRFDGFLNHFKTSTH-GFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWV 232
             R           +N+   +     F I+G  G+GK+TLA+  +ND +V ++F+ ++WV
Sbjct: 160 YGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWV 219

Query: 233 CVKEEFDTQRLIKEML-SFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLG 292
           CV ++FD +RLIK ++ +        ++L   Q    +Q+ L  K++LLV  D  + N  
Sbjct: 220 CVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQK--KLQELLNGKRYLLVLDD--VWNDD 279

Query: 293 INNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNINND 352
           +   +    +L +G  G+ I+ TT+ E++   +G                  +P +++N 
Sbjct: 280 LEKWAKLRAVLTVGARGASILATTRLEKVGSIMG----------------TLQPYHLSN- 339

Query: 353 YVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEE 412
                                        LS  +SL LF   AF + +EA + NL  I +
Sbjct: 340 -----------------------------LSPHDSLLLFMQRAFGQQKEA-NPNLVAIGK 399

Query: 413 VIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVID-KLWAQDEGEDMSILPTLRLCYDLMP 472
            I +KC  VPLA K LGGLL  K   +E   V D ++W+  + E  SILP LRL Y  +P
Sbjct: 400 EIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDES-SILPALRLSYHHLP 459

Query: 473 SHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKELCSRCFLQ 532
             L++CF  C+   KD  +   ++I LW+A G L  +    LE +G   + EL  R F Q
Sbjct: 460 LDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQ 519

Query: 533 ELEEYGLGYWFKLHPLIQKLARI----------ITQKEVFPVTEPKSIAF-TVRDKVPPS 592
           E+E      +FK+H LI  LA            I +  V       SI F  V     PS
Sbjct: 520 EIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSSYSPS 579

Query: 593 AFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPN 652
                  +KKF +LR+L L  + L ++P+++G L  LRYLDL  N   + LP  +C L N
Sbjct: 580 ------LLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN-NFRSLPERLCKLQN 625

Query: 653 LQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFL 689
           LQTL + +C +L  LP    KL  LR+L V    L      +G +T L+ L
Sbjct: 640 LQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTL 625

BLAST of Lsi03G016700 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 6.3e-56
Identity = 196/661 (29.65%), Postives = 305/661 (46.14%), Query Frame = 0

Query: 54  LKAILMDA-EKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKA--YKQKAIGKQVFVSSL 113
           ++A+L DA EK+     + +WL++L     +++  + + K +A  +KQ  +G+  +    
Sbjct: 41  IQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGR--YHPRT 100

Query: 114 SSSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMKPSWQLLYPL 173
            +   +  ++MK++ + LD IA +   F+L             E      +  + L  P 
Sbjct: 101 ITFCYKVGKRMKEMMEKLDAIAEERRNFHLDE--------RIIERQAARRQTGFVLTEPK 160

Query: 174 TNARQVFHGSRFDGFLNHFKTSTH-GFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLW 233
              R+          +N+   S       I+G  G+GK+TLA+  +ND ++ E+F+ ++W
Sbjct: 161 VYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIW 220

Query: 234 VCVKEEFDTQRLIKEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLG 293
           VCV ++FD +RLIK ++  S    +  ++    L   +Q+ L  K++ LV  D  + N  
Sbjct: 221 VCVSDDFDEKRLIKAIVE-SIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDD--VWNED 280

Query: 294 INNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNINND 353
                    +L +G  G+ I++TT+ E+I   +G  +LY+                    
Sbjct: 281 QEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQLYQ-------------------- 340

Query: 354 YVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEE 413
                                     +  LS+E+   LFK  AF    E  S  L +I +
Sbjct: 341 --------------------------LSNLSQEDCWLLFKQRAFCHQTET-SPKLMEIGK 400

Query: 414 VIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVID-KLWAQDEGEDMSILPTLRLCYDLMP 473
            I +KC  VPLA K LGGLL  K   +E   V D ++W   + E+ S+LP LRL Y  +P
Sbjct: 401 EIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDEN-SVLPALRLSYHHLP 460

Query: 474 SHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKELCSRCFLQ 533
             L++CF  C+   KD  +    +I LW+A   L  +    LE +G   + EL  R F Q
Sbjct: 461 LDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQ 520

Query: 534 ELEEYGLGYWFKLHPLIQKLA-------------RIITQKE----VFPVTEPK---SIAF 593
           E+E      +FK+H LI  LA             R I  K+    +F VT  K   SI F
Sbjct: 521 EIEVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGF 580

Query: 594 T-VRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKR 653
           + V     PS F      K+F +LR+L L N+   ++P++VG L  LRYLDL GNK I  
Sbjct: 581 SEVVSSYSPSLF------KRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNK-ICS 633

Query: 654 LPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRF 689
           LP  +C L NLQTL L +C +L  LP    KL  LR L +    L      +G +T L+ 
Sbjct: 641 LPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKT 633

BLAST of Lsi03G016700 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 5.0e-53
Identity = 181/657 (27.55%), Postives = 308/657 (46.88%), Query Frame = 0

Query: 45  RRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKA--YKQKAI 104
           +R+     +++A+L DA EK+     L +WL++L     +++  + E+K KA  + Q   
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEYKTKATRFLQSEY 91

Query: 105 GKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMK 164
           G+  +   +   + +  ++M ++   L+ IA +  +F+L   +      +  E  + L +
Sbjct: 92  GR--YHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQE-KIIERQAATRETGSVLTE 151

Query: 165 PSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVGDAGIGKSTLAKFFYNDPKVV 224
           P    +Y     +        +   +  K S      I+G  G+GK+TL++  +ND +V 
Sbjct: 152 PQ---VYGRDKEKDEIVKILINTASDAQKLSV---LPILGMGGLGKTTLSQMVFNDQRVT 211

Query: 225 ENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQ 284
           E F  ++W+C+ ++F+ +RLIK ++  S    +  ++    L   +Q+ L  K++ LV  
Sbjct: 212 ERFYPKIWICISDDFNEKRLIKAIVE-SIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLD 271

Query: 285 DISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDAT 344
           D  + N   +  +    +L +G  G+ ++ TT+ E++   +G                  
Sbjct: 272 D--VWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSIMG----------------TL 331

Query: 345 KPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQS 404
           +P  ++N                              LS E+   LF   AF   QE  +
Sbjct: 332 QPYELSN------------------------------LSPEDCWFLFMQRAFGH-QEEIN 391

Query: 405 SNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVIDK-LWAQDEGEDMSILPTL 464
            NL  I + I +KC  VPLA K LGG+L  K    E   V D  +W   + E  SILP L
Sbjct: 392 PNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDES-SILPAL 451

Query: 465 RLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKE 524
           RL Y  +P  L++CF+ C+   KD  ++  ++I  W+A G L  +    LE +G   + E
Sbjct: 452 RLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNE 511

Query: 525 LCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQ--------KEVFPVTEPKSIAFTVRD 584
           L  R F QE+E      +FK+H LI  LA  +          +E+    +   ++    +
Sbjct: 512 LYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAE 571

Query: 585 KVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSI 644
            V   +  + + ++KF +LR+L L N+NL ++P+++G L  LRYLDL GN RI+ LP  +
Sbjct: 572 VV---SSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRL 625

Query: 645 CYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLA 690
           C L NLQTL L  C +L  LP    KL  LR L +    L      +G +T L+ L+
Sbjct: 632 CKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLS 625

BLAST of Lsi03G016700 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.6e-51
Identity = 185/660 (28.03%), Postives = 301/660 (45.61%), Query Frame = 0

Query: 45  RRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKA--YKQKAI 104
           +R+     +++A+L DA EK+  +  L +WL++L     +++  + E+K KA  + Q   
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEY 91

Query: 105 GKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMK 164
           G+  +   +   + +  ++M ++   L  IA +   F+L      H  +   +   R  +
Sbjct: 92  GR--YHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHL------HEKIVERQAVRR--E 151

Query: 165 PSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTH-GFFHIVGDAGIGKSTLAKFFYNDPKV 224
               L  P    R           +N+   + H     I+G  G+GK+TLA+  +ND +V
Sbjct: 152 TGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRV 211

Query: 225 VENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVF 284
            E+F S++W+CV E+FD +RLIK ++     +     +    L   +Q+ L  K++LLV 
Sbjct: 212 TEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVL 271

Query: 285 QDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDA 344
            D  + N      +    +L +G  G+ ++ TT+ E++   +G                 
Sbjct: 272 DD--VWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSIMG----------------T 331

Query: 345 TKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQ 404
            +P  ++N                              LS+E+   LF   AF   QE  
Sbjct: 332 LQPYELSN------------------------------LSQEDCWLLFMQRAFGH-QEEI 391

Query: 405 SSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVIDK-LWAQDEGEDMSILPT 464
           + NL  I + I +K   VPLA K LGG+L  K        V D  +W   + E  SILP 
Sbjct: 392 NPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDES-SILPA 451

Query: 465 LRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFK 524
           LRL Y  +P  L++CF  C+   KD  +    +I LW+A G L  +    LE +G+  +K
Sbjct: 452 LRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWK 511

Query: 525 ELCSRCFLQELEEYGLGYWFKLHPLIQKLARI----------ITQKEVFPVTEPKSIAFT 584
           EL  R F QE+E      +FK+H LI  LA            I +      T   SI F 
Sbjct: 512 ELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGF- 571

Query: 585 VRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLP 644
                    F     ++KF +LR+L LG++   ++P+++G L  LRYL+L G+  ++ LP
Sbjct: 572 ----AEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLP 625

Query: 645 NSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANK-LYLHKNGVGTMTSLRFL 689
             +C L NLQTL L  C+ L  LP +  KL  LR L +  ++ L      +G++T L+ L
Sbjct: 632 KQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTL 625

BLAST of Lsi03G016700 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 5.9e-46
Identity = 177/707 (25.04%), Postives = 302/707 (42.72%), Query Frame = 0

Query: 35  RELKNCPNYLRRMEYTILSLKAILMDAEKEEY-SPYLCSWLKELQNVFTQIEGFIYEFKG 94
           REL    N L R+   +L++ A+L+DAE+++  +P +  W+ EL++V    E  + +   
Sbjct: 32  RELNE--NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDI-- 91

Query: 95  KAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLD------EIASQMYEFNLTNMETTH 154
                +A+   +   S SS+++   +    L D LD      E   +     L  + +  
Sbjct: 92  ---ATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQR 151

Query: 155 SFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHG------FFHIVG 214
           + L   E++  + K        L +  +VF        +  F    +G         IVG
Sbjct: 152 NILGLKELTAMIPKQRLPTT-SLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVG 211

Query: 215 DAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEK 274
             G+GK+TL++  YND  V   F +++W  V EEFD  ++ K++          +++T +
Sbjct: 212 IGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVY---------ESVTSR 271

Query: 275 QLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADA 334
              FT                    +L +    +   L G G P   ++    NE  AD 
Sbjct: 272 PCEFT--------------------DLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 331

Query: 335 IGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSK 394
             L + + + +Q    L  T+   + +   ++                   + N++ LS 
Sbjct: 332 DLLRQPFIHAAQGSQILVTTRSQRVASIMCAVH------------------VHNLQPLSD 391

Query: 395 ENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSV 454
            +  SLF    F   +   +  +  + E I  KC  +PLA+K LGG+L  +  + E   V
Sbjct: 392 GDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERV 451

Query: 455 IDKLWAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGL 514
           +         +  ++LP LR+ Y  +P+HL+RCF  CS   K      + V+ LW+A+G 
Sbjct: 452 LSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGF 511

Query: 515 LSEENYS-SLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQKE---- 574
           L +   S +LE+LG  YF EL SR  LQ+ +       + +H  I +LA+  + +     
Sbjct: 512 LQQTRSSKNLEELGNEYFSELESRSLLQKTKTR-----YIMHDFINELAQFASGEFSSKF 571

Query: 575 ----VFPVTEPKSIAFTVRDK-VPPSAF-----------------------------LAE 634
                  V+E       +RD    P  F                             ++E
Sbjct: 572 EDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQMVSE 631

Query: 635 TCIKKFNNLRLLYLGNANLREI-PNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTL 689
             +     LR+L L +  +  + P+    ++  R+LDL     +++LP S+CY+ NLQTL
Sbjct: 632 KLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEKLPKSLCYMYNLQTL 677

BLAST of Lsi03G016700 vs. ExPASy TrEMBL
Match: A0A1S3B7G0 (putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC103486834 PE=4 SV=1)

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 660/825 (80.00%), Postives = 721/825 (87.39%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDAEKEEYSPYLCSWLKELQNVF 81
           MA RI  QAENILRELK  P YLRRM+YT+LSLK ILMDAEKEEYS +L  WL+ELQNVF
Sbjct: 1   MACRIYNQAENILRELKKFPKYLRRMQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVF 60

Query: 82  TQIEGFIYEFKGKAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLT 141
           +QIEG +YEF GK  KQ+A GK VFV S +SS+IE T+KM KLCD+LDEIASQMY+FNLT
Sbjct: 61  SQIEGLLYEFNGKVKKQEATGKWVFVLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLT 120

Query: 142 NMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVG 201
           NMETTHSFLSATEVSTRLMKPSWQLLYPLTNA +VFH  R+  FL+HFK ST GFFHIVG
Sbjct: 121 NMETTHSFLSATEVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVG 180

Query: 202 DAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEK 261
           + GIGK+TLAKFFYNDP+VV+ F SRLW+CVKEEFD +RLIKEML FS+CQAT DNLTEK
Sbjct: 181 EPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEK 240

Query: 262 QLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADA 321
           QLCF VQQ+LRDK FLLVFQDISIKNLG  +CS+F +LLGMGNPGSKIIVTTQNEEIA+A
Sbjct: 241 QLCFAVQQFLRDKTFLLVFQDISIKNLG--DCSIFKSLLGMGNPGSKIIVTTQNEEIAEA 300

Query: 322 IGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSK 381
           I LGKL KNESQ V   +AT+PS++NND            E YK+NMT+Q IF VERLSK
Sbjct: 301 IRLGKLCKNESQEVPSPEATEPSDVNND------------EAYKDNMTYQKIFEVERLSK 360

Query: 382 ENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSV 441
           ENSLSLFKVHAFTE QEAQ  NLTKI+EVIEQKCH VPLAIKCLGGLL SKTSIAE N V
Sbjct: 361 ENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGV 420

Query: 442 IDKLWAQDEGED--MSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQ 501
           IDKLW  +EGED   SILP LRLCYD MPSHLQRCFL CSQLTKDRILSSNDVIQLWIA 
Sbjct: 421 IDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIAS 480

Query: 502 GLLSEENYSSLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQKEVFP 561
            LL +ENY SLEK+GENYFKELCSRCFLQELEEYGLGYWFKLHPLI+KLARI+TQK+VFP
Sbjct: 481 DLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFP 540

Query: 562 VTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDL 621
           V   K IAFTVRDKVPPSAFLA TCI KF  +RLLYLGNANLREIPNAV  L QLRYLDL
Sbjct: 541 VANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDL 600

Query: 622 QGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGV 681
           QGNKR+KRLPNSI  L NLQTLILASCSALEELP+ I++LI+LRYLWVTAN L LHKNGV
Sbjct: 601 QGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGV 660

Query: 682 GTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYDCKTLKLLPNEIGSLISLQNLVIW 741
           GTMTSLRFLAIGGC+NLQDLFEQPSCLVRLETLMIYD KTLKLLPNEIG+LISL+NLVIW
Sbjct: 661 GTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIW 720

Query: 742 SCKRLTLTLKGVEFRLRRFTIRELPKVNKLPEWLQRFTETLRVLEIIDCPIELKDDKFKS 801
           SCK+LTLTLKGVEFRLRRFTIRELP+V KLPEW+QRFTE+LRVLEIIDCPIELKDD+FKS
Sbjct: 721 SCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKS 780

Query: 802 YKALERISIHGDVRVKNSNDEYSVEFKNSVSRRQVKKEMKTCSYY 845
           YK+LER+SIHG VR+KN N EY ++F+NSV RR+V KEMKTC YY
Sbjct: 781 YKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMKTCYYY 810

BLAST of Lsi03G016700 vs. ExPASy TrEMBL
Match: A0A5A7UDS9 (Putative disease resistance protein RGA4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006080 PE=4 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 641/800 (80.12%), Postives = 702/800 (87.75%), Query Frame = 0

Query: 47  MEYTILSLKAILMDAEKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKAYKQKAIGKQVF 106
           M+YT+LSLK ILMDAEKEEYS +L  WL+ELQNVF+QIEG +YEF GK  KQ+A GK VF
Sbjct: 1   MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60

Query: 107 VSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMKPSWQL 166
           V S +SS+IE T+KM KLCD+LDEIASQMY+FNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 61  VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120

Query: 167 LYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSS 226
           LYPLTNA +VFH  R+  FL+HFK ST GFFHIVG+ GIGK+TLAKFFYNDP+VV+ F S
Sbjct: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180

Query: 227 RLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIK 286
           RLW+CVKEEFD +RLIKEML FS+CQAT DNLTEKQLCF VQQ+LRDK FLLVFQDISIK
Sbjct: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240

Query: 287 NLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNI 346
           NLG  +CS+F +LLGMGNPGSKIIVTTQNEEIA+AI LGKL KNESQ V   +AT+PS++
Sbjct: 241 NLG--DCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDV 300

Query: 347 NNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTK 406
           NND            E YK+NMT+Q IF VERLSKENSLSLFKVHAFTE QEAQ  NLTK
Sbjct: 301 NND------------EAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTK 360

Query: 407 IEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVIDKLWAQDEGED--MSILPTLRLCY 466
           I+EVIEQKCH VPLAIKCLGGLL SKTSIAE N VIDKLW  +EGED   SILP LRLCY
Sbjct: 361 IQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCY 420

Query: 467 DLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKELCSR 526
           D MPSHLQRCFL CSQLTKDRILSSNDVIQLWIA  LL +ENY SLEK+GENYFKELCSR
Sbjct: 421 DRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSR 480

Query: 527 CFLQELEEYGLGYWFKLHPLIQKLARIITQKEVFPVTEPKSIAFTVRDKVPPSAFLAETC 586
           CFLQELEEYGLGYWFKLHPLI+KLARI+TQK+VFPV   K IAFTVRDKVPPSAFLA TC
Sbjct: 481 CFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTC 540

Query: 587 IKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILA 646
           I KF  +RLLYLGNANLREIPNAV  L QLRYLDLQGNKR+KRLPNSI  L NLQTLILA
Sbjct: 541 IDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILA 600

Query: 647 SCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPS 706
           SCSALEELP+ I++LI+LRYLWVTAN L LHKNGVGTMTSLRFLAIGGC+NLQDLFEQPS
Sbjct: 601 SCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPS 660

Query: 707 CLVRLETLMIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLTLKGVEFRLRRFTIRELP 766
           CLVRLETLMIYD KTLKLLPNEIG+LISL+NLVIWSCK+LTLTLKGVEFRLRRFTIRELP
Sbjct: 661 CLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELP 720

Query: 767 KVNKLPEWLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRVKNSNDEYSVE 826
           +V KLPEW+QRFTE+LRVLEIIDCPIELKDD+FKSYK+LER+SIHG VR+KN N EY ++
Sbjct: 721 RVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCID 780

Query: 827 FKNSVSRRQVKKEMKTCSYY 845
           F+NSV RR+V KEMKTC YY
Sbjct: 781 FQNSVRRREVTKEMKTCYYY 785

BLAST of Lsi03G016700 vs. ExPASy TrEMBL
Match: A0A0A0LF99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G814390 PE=4 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 627/825 (76.00%), Postives = 691/825 (83.76%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDAEKEEYSPYLCSWLKELQNVF 81
           MA  I EQAENIL ELK  P YLRRM+YT+LSLK IL DAEKEEY   L  WL++LQ+VF
Sbjct: 1   MACCIYEQAENILIELKKFPMYLRRMQYTMLSLKTILKDAEKEEYRHCLNDWLQKLQSVF 60

Query: 82  TQIEGFIYEFKGKAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLT 141
            QIE  +YE   +  KQ+A GK VF+ S + SQI+ T+KM KLCD+LDEIAS MY FNLT
Sbjct: 61  LQIEELLYESNREVKKQEATGKWVFLPSFNFSQIDQTKKMMKLCDDLDEIASHMYGFNLT 120

Query: 142 NMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVG 201
           NMETTHSFLSATEVSTRLMKPSWQLLY LTNA +VF   R+  FL+HFK STHG FHIVG
Sbjct: 121 NMETTHSFLSATEVSTRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVG 180

Query: 202 DAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEK 261
           + GIGK+TLAKFFYN+  +V  F SRLW+CVKEEFD QRLIKEMLSFS+CQ TCDNLTEK
Sbjct: 181 EPGIGKTTLAKFFYNN--LVNTFPSRLWICVKEEFDPQRLIKEMLSFSHCQVTCDNLTEK 240

Query: 262 QLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADA 321
           QLCF VQQ+LRDKKFL+VFQDISIKNLG  NCS+F +LLGMGN GSKIIVTTQNE+IADA
Sbjct: 241 QLCFAVQQFLRDKKFLIVFQDISIKNLG--NCSIFKSLLGMGNRGSKIIVTTQNEKIADA 300

Query: 322 IGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSK 381
           +GL KLYKNESQVV   +ATKPS++N                 K+NM HQTIF VERLSK
Sbjct: 301 VGLKKLYKNESQVVPSPEATKPSDVN-----------------KDNMKHQTIFKVERLSK 360

Query: 382 ENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSV 441
           ENSLSLFKVHAFTE QEAQ  NLTKI+EVIEQKCH VPLAIKCLGGLL SKTSIAE N V
Sbjct: 361 ENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGV 420

Query: 442 IDKLWAQDEGED--MSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQ 501
           IDKLW  +E ED   SILPTLRLCYD MPSHLQRCFL CSQL KDRILSSNDVIQLWIA 
Sbjct: 421 IDKLWEHEEEEDGNKSILPTLRLCYDQMPSHLQRCFLYCSQLKKDRILSSNDVIQLWIAS 480

Query: 502 GLLSEENYSSLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQKEVFP 561
            LL +ENY SLEK+GENYFKELCSRCFLQELEEYG GYWFKLHPLI+KLAR++TQK+VF 
Sbjct: 481 DLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWFKLHPLIEKLARLLTQKQVFE 540

Query: 562 VTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDL 621
           VT+ +SIAFT+RDKVPPSAFLA  CI KF  LRLL+LGNANL+ IP+AV  L QLRYLDL
Sbjct: 541 VTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGNANLQGIPSAVENLVQLRYLDL 600

Query: 622 QGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGV 681
           QGNK+IKRLPNSI  L NLQTLILASCSAL+ELP+DIR+L +LRYLWVTAN L LHKNGV
Sbjct: 601 QGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQLTNLRYLWVTANNLRLHKNGV 660

Query: 682 GTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYDCKTLKLLPNEIGSLISLQNLVIW 741
           GTMTSLRFLAIGGC NLQDLF++PSCLVRLETLMIYDCKTLK LPNEIGSLISL+NLVIW
Sbjct: 661 GTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCKTLKSLPNEIGSLISLKNLVIW 720

Query: 742 SCKRLTLTLKGVEFRLRRFTIRELPKVNKLPEWLQRFTETLRVLEIIDCPIELKDDKFKS 801
           SCK+LTLTLKGVEFRL+RFTIRELP V KLPEW QRFTETLRVLEIIDCPIE  DD  KS
Sbjct: 721 SCKKLTLTLKGVEFRLQRFTIRELPIVKKLPEWTQRFTETLRVLEIIDCPIEWNDDVLKS 780

Query: 802 YKALERISIHGDVRVKNSNDEYSVEFKNSVSRRQVKKEMKTCSYY 845
           YK+LER SIHG VR KN    Y+++++N V  R+VKKE+KTC YY
Sbjct: 781 YKSLERFSIHGAVRTKNQIGGYNIDYRNFVRSRKVKKEVKTCVYY 803

BLAST of Lsi03G016700 vs. ExPASy TrEMBL
Match: A0A1S4DUI6 (putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC107990605 PE=4 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 4.7e-272
Identity = 524/824 (63.59%), Postives = 623/824 (75.61%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNV 81
           MAY I  +AENIL ELKNCPNY RR+EYT+LSLK+ILMDA EK+E+S +L +WLKELQNV
Sbjct: 1   MAYCIYYRAENILSELKNCPNYPRRIEYTMLSLKSILMDAEEKQEHSRHLRNWLKELQNV 60

Query: 82  FTQIEGFIYEFKGKAYKQKAIGKQVFVS-SLSSSQIEHTQKMKKLCDNLDEIASQMYEFN 141
           F QIEGFI E K + YK + IGKQV    S S  Q   TQKM+KL D+LD + ++MYEF+
Sbjct: 61  FFQIEGFIDELKEEVYKTEGIGKQVLAPFSFSICQRARTQKMEKLFDHLDAVVAKMYEFD 120

Query: 142 L-------TNMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTS 201
           L         METT SFL+ATEVST LMKPSW++LYP T+A + +   R+   LN FK S
Sbjct: 121 LKERHTGAIKMETTDSFLTATEVSTSLMKPSWKVLYPSTSAPKSYQHERYREILNDFKES 180

Query: 202 THGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQ 261
           T GFFHIVG+AGIGKSTLAKF YNDP+V E F+SRLWVCVKEEFDTQRL+ E+L+FSY  
Sbjct: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEEMFTSRLWVCVKEEFDTQRLMTEILNFSYSP 240

Query: 262 ATCDNLTEKQLCFT--VQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKII 321
           ATCDNLTE + C T  VQQYLR+K FLLVFQD+SIKNL  +N S+FT+LL MG PGSKII
Sbjct: 241 ATCDNLTETKSCPTVQVQQYLREKTFLLVFQDLSIKNL--DNSSLFTSLLRMGKPGSKII 300

Query: 322 VTTQNEEIADAIGLGKLY-----------KNESQVVSFLDATKPSNINNDYVSIQANPVT 381
           VTTQNEEIA+AI L K+            +NESQ        + SN+NN   S QANP+ 
Sbjct: 301 VTTQNEEIANAIKLPKVMVEVNKVEQQSEQNESQTALDAVTIETSNVNNAGESTQANPL- 360

Query: 382 DPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVP 441
             +    ++T+QTIF V+RLS+E+SLSLFK +A     + +      I E +E KC+ VP
Sbjct: 361 --DKLDQSITYQTIFKVKRLSEEDSLSLFKDYASIVEDDKEG-----IIETLE-KCNGVP 420

Query: 442 LAIKCLGGLLSSKTSIAEKNSVIDKLWAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCS 501
           LAIKCLG +LS +TS  +     D+   ++  E  S    L+LCY+ MPSHL+ CFL CS
Sbjct: 421 LAIKCLGSMLSLETSATKWMEDTDQ--QEEVNESSSTFSILKLCYNQMPSHLKPCFLYCS 480

Query: 502 QLTKDRILSSNDVIQLWIAQGLL--SEENYSSLEKLGENYFKELCSRCFLQELEEYGLGY 561
           QL  D ILSSNDVIQLW+A GLL   +ENY SLE +GE YFKELCSRCFLQ++EEYGLGY
Sbjct: 481 QLQTDSILSSNDVIQLWMANGLLHSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGY 540

Query: 562 WFKLHPLIQKLARIITQKE------VFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNL 621
           WFK+HPLI+KLARI+ Q++      + PVT+  SIAF VRD+VP S+FLAE CI KF  L
Sbjct: 541 WFKMHPLIRKLARILIQEQTKDLICIKPVTKVTSIAFPVRDEVPSSSFLAEKCISKFQYL 600

Query: 622 RLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEE 681
           RLL+LG  +L+EIPN + TL  LRYLDLQGNK+IKRLPN+IC L  LQTLILASC AL+E
Sbjct: 601 RLLHLGYTDLQEIPNTIETLKHLRYLDLQGNKKIKRLPNAICNLQYLQTLILASCFALQE 660

Query: 682 LPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLET 741
           LP DI KL  LRYLWVT+N L+LHKNGVGTM SLRFLAIGGCD LQDLFEQPSCLVRLET
Sbjct: 661 LPKDIWKLSKLRYLWVTSNNLHLHKNGVGTMNSLRFLAIGGCDKLQDLFEQPSCLVRLET 720

Query: 742 LMIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLT-LKGVEFRLRRFTIRELPKVNKLP 801
           LMIY+CK+L+LLPNE+GSLISLQNLVIWSC++LTL  L+ V+F L+RFTIRELPKVNKLP
Sbjct: 721 LMIYNCKSLQLLPNEMGSLISLQNLVIWSCEQLTLKGLEKVDFSLQRFTIRELPKVNKLP 780

Query: 802 EWLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRV 815
           EWLQR TETLRVLEIIDCPI ++++  K YKA+E   I G V V
Sbjct: 781 EWLQRSTETLRVLEIIDCPIRVEEEGIKMYKAVESKIIQGAVDV 811

BLAST of Lsi03G016700 vs. ExPASy TrEMBL
Match: A0A0A0LEM8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G814400 PE=4 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 2.2e-269
Identity = 520/823 (63.18%), Postives = 622/823 (75.58%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNV 81
           MAY I  +AENIL ELKN PNY RR+EYT+LSLK+ILMDA EK+E S  L +WL+ELQNV
Sbjct: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60

Query: 82  FTQIEGFIYEFKGKAYKQKAIGKQVFVS-SLSSSQIEHTQKMKKLCDNLDEIASQMYEFN 141
           F+QIEGFI E K +AY  + IGKQV    S SS+QI  T KM+KL D+L+E+A++MYEFN
Sbjct: 61  FSQIEGFIDEHKEEAY--EGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120

Query: 142 LT-------NMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTS 201
           LT         ETT+SFL+ATEVSTRLMKPSW++LYPLTNA + +   R+   LN FK  
Sbjct: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180

Query: 202 THGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQ 261
           T GFFHIVG+AGIGKSTLAKF YNDP+V   F SRLWVCVKEEFDTQRL+KE+L+FSY  
Sbjct: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240

Query: 262 ATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVT 321
           ATCDNLT K LC T  QYLR++ FLLVFQD+SIKNL  +NCS+FT+LL MG PGSKIIVT
Sbjct: 241 ATCDNLTTK-LCPT-DQYLRERTFLLVFQDLSIKNL--DNCSLFTSLLMMGKPGSKIIVT 300

Query: 322 TQNEEIADAIGLGKLYK-------NESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYK 381
           TQNEEIA+AI L  +YK       N SQ        + +N+NN    +QANP+   +   
Sbjct: 301 TQNEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKID--- 360

Query: 382 NNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCL 441
            ++  QTIF V+RLS+++SLSLFK +A T   E    ++ K      +KC+ +PLAIKCL
Sbjct: 361 QSIPSQTIFKVKRLSEKDSLSLFKDYAST--YEGNEKDIMK----TLKKCNGIPLAIKCL 420

Query: 442 GGLLSSKTSIAEKNSVIDKLWAQD------EGEDMSILPTLRLCYDLMPSHLQRCFLSCS 501
           G +LS      +        W +D      + E  S    L+LCY+ MPSHL+RCFL CS
Sbjct: 421 GSMLSLGPPATK--------WMEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCS 480

Query: 502 QLTKDRILSSNDVIQLWIAQGLL--SEENYSSLEKLGENYFKELCSRCFLQELEEYGLGY 561
           QL  D ILSSNDVIQLW+A GLL   +ENY SLE +GE YFKELCSRCFLQ++EEYGLGY
Sbjct: 481 QLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGY 540

Query: 562 WFKLHPLIQKLARIITQK-----EVFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLR 621
           WFK+HPLI++LAR++ ++      + PVT   SIAF VRD+VP S+FLAE CI KF +LR
Sbjct: 541 WFKMHPLIRELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLR 600

Query: 622 LLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEEL 681
           LLYLG+ +L+EIPN + TL  L YLDLQGNK IKRLPN+IC L +LQTLILASCSALEEL
Sbjct: 601 LLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEEL 660

Query: 682 PDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETL 741
           P DI KL +LRYLWVT+NKL LHKNGVGTMTSLRFLAIGGCD LQDLFE+PSCLVRLETL
Sbjct: 661 PKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETL 720

Query: 742 MIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLT-LKGVEFRLRRFTIRELPKVNKLPE 801
           MIYDC +L+LLPNE+GSLISLQNLVIWSCK+LTL  L+ V+F L+RFTIRELP+VNKLPE
Sbjct: 721 MIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPE 780

Query: 802 WLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRV 815
           WLQR TETLRVLEIIDCPI+++++  K YKA+E   I G V +
Sbjct: 781 WLQRSTETLRVLEIIDCPIKVEEEGIKMYKAVESKIIQGAVDI 800

BLAST of Lsi03G016700 vs. NCBI nr
Match: XP_008443166.1 (PREDICTED: putative disease resistance protein RGA4 [Cucumis melo])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 660/825 (80.00%), Postives = 721/825 (87.39%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDAEKEEYSPYLCSWLKELQNVF 81
           MA RI  QAENILRELK  P YLRRM+YT+LSLK ILMDAEKEEYS +L  WL+ELQNVF
Sbjct: 1   MACRIYNQAENILRELKKFPKYLRRMQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVF 60

Query: 82  TQIEGFIYEFKGKAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLT 141
           +QIEG +YEF GK  KQ+A GK VFV S +SS+IE T+KM KLCD+LDEIASQMY+FNLT
Sbjct: 61  SQIEGLLYEFNGKVKKQEATGKWVFVLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLT 120

Query: 142 NMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVG 201
           NMETTHSFLSATEVSTRLMKPSWQLLYPLTNA +VFH  R+  FL+HFK ST GFFHIVG
Sbjct: 121 NMETTHSFLSATEVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVG 180

Query: 202 DAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEK 261
           + GIGK+TLAKFFYNDP+VV+ F SRLW+CVKEEFD +RLIKEML FS+CQAT DNLTEK
Sbjct: 181 EPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEK 240

Query: 262 QLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADA 321
           QLCF VQQ+LRDK FLLVFQDISIKNLG  +CS+F +LLGMGNPGSKIIVTTQNEEIA+A
Sbjct: 241 QLCFAVQQFLRDKTFLLVFQDISIKNLG--DCSIFKSLLGMGNPGSKIIVTTQNEEIAEA 300

Query: 322 IGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSK 381
           I LGKL KNESQ V   +AT+PS++NND            E YK+NMT+Q IF VERLSK
Sbjct: 301 IRLGKLCKNESQEVPSPEATEPSDVNND------------EAYKDNMTYQKIFEVERLSK 360

Query: 382 ENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSV 441
           ENSLSLFKVHAFTE QEAQ  NLTKI+EVIEQKCH VPLAIKCLGGLL SKTSIAE N V
Sbjct: 361 ENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGV 420

Query: 442 IDKLWAQDEGED--MSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQ 501
           IDKLW  +EGED   SILP LRLCYD MPSHLQRCFL CSQLTKDRILSSNDVIQLWIA 
Sbjct: 421 IDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIAS 480

Query: 502 GLLSEENYSSLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQKEVFP 561
            LL +ENY SLEK+GENYFKELCSRCFLQELEEYGLGYWFKLHPLI+KLARI+TQK+VFP
Sbjct: 481 DLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFP 540

Query: 562 VTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDL 621
           V   K IAFTVRDKVPPSAFLA TCI KF  +RLLYLGNANLREIPNAV  L QLRYLDL
Sbjct: 541 VANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDL 600

Query: 622 QGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGV 681
           QGNKR+KRLPNSI  L NLQTLILASCSALEELP+ I++LI+LRYLWVTAN L LHKNGV
Sbjct: 601 QGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGV 660

Query: 682 GTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYDCKTLKLLPNEIGSLISLQNLVIW 741
           GTMTSLRFLAIGGC+NLQDLFEQPSCLVRLETLMIYD KTLKLLPNEIG+LISL+NLVIW
Sbjct: 661 GTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIW 720

Query: 742 SCKRLTLTLKGVEFRLRRFTIRELPKVNKLPEWLQRFTETLRVLEIIDCPIELKDDKFKS 801
           SCK+LTLTLKGVEFRLRRFTIRELP+V KLPEW+QRFTE+LRVLEIIDCPIELKDD+FKS
Sbjct: 721 SCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKS 780

Query: 802 YKALERISIHGDVRVKNSNDEYSVEFKNSVSRRQVKKEMKTCSYY 845
           YK+LER+SIHG VR+KN N EY ++F+NSV RR+V KEMKTC YY
Sbjct: 781 YKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMKTCYYY 810

BLAST of Lsi03G016700 vs. NCBI nr
Match: KAA0053942.1 (putative disease resistance protein RGA4 [Cucumis melo var. makuwa] >TYK25462.1 putative disease resistance protein RGA4 [Cucumis melo var. makuwa])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 641/800 (80.12%), Postives = 702/800 (87.75%), Query Frame = 0

Query: 47  MEYTILSLKAILMDAEKEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKAYKQKAIGKQVF 106
           M+YT+LSLK ILMDAEKEEYS +L  WL+ELQNVF+QIEG +YEF GK  KQ+A GK VF
Sbjct: 1   MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60

Query: 107 VSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMKPSWQL 166
           V S +SS+IE T+KM KLCD+LDEIASQMY+FNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 61  VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120

Query: 167 LYPLTNARQVFHGSRFDGFLNHFKTSTHGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSS 226
           LYPLTNA +VFH  R+  FL+HFK ST GFFHIVG+ GIGK+TLAKFFYNDP+VV+ F S
Sbjct: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180

Query: 227 RLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIK 286
           RLW+CVKEEFD +RLIKEML FS+CQAT DNLTEKQLCF VQQ+LRDK FLLVFQDISIK
Sbjct: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240

Query: 287 NLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNI 346
           NLG  +CS+F +LLGMGNPGSKIIVTTQNEEIA+AI LGKL KNESQ V   +AT+PS++
Sbjct: 241 NLG--DCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDV 300

Query: 347 NNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTK 406
           NND            E YK+NMT+Q IF VERLSKENSLSLFKVHAFTE QEAQ  NLTK
Sbjct: 301 NND------------EAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTK 360

Query: 407 IEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSVIDKLWAQDEGED--MSILPTLRLCY 466
           I+EVIEQKCH VPLAIKCLGGLL SKTSIAE N VIDKLW  +EGED   SILP LRLCY
Sbjct: 361 IQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCY 420

Query: 467 DLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKELCSR 526
           D MPSHLQRCFL CSQLTKDRILSSNDVIQLWIA  LL +ENY SLEK+GENYFKELCSR
Sbjct: 421 DRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSR 480

Query: 527 CFLQELEEYGLGYWFKLHPLIQKLARIITQKEVFPVTEPKSIAFTVRDKVPPSAFLAETC 586
           CFLQELEEYGLGYWFKLHPLI+KLARI+TQK+VFPV   K IAFTVRDKVPPSAFLA TC
Sbjct: 481 CFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTC 540

Query: 587 IKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILA 646
           I KF  +RLLYLGNANLREIPNAV  L QLRYLDLQGNKR+KRLPNSI  L NLQTLILA
Sbjct: 541 IDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILA 600

Query: 647 SCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPS 706
           SCSALEELP+ I++LI+LRYLWVTAN L LHKNGVGTMTSLRFLAIGGC+NLQDLFEQPS
Sbjct: 601 SCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPS 660

Query: 707 CLVRLETLMIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLTLKGVEFRLRRFTIRELP 766
           CLVRLETLMIYD KTLKLLPNEIG+LISL+NLVIWSCK+LTLTLKGVEFRLRRFTIRELP
Sbjct: 661 CLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELP 720

Query: 767 KVNKLPEWLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRVKNSNDEYSVE 826
           +V KLPEW+QRFTE+LRVLEIIDCPIELKDD+FKSYK+LER+SIHG VR+KN N EY ++
Sbjct: 721 RVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCID 780

Query: 827 FKNSVSRRQVKKEMKTCSYY 845
           F+NSV RR+V KEMKTC YY
Sbjct: 781 FQNSVRRREVTKEMKTCYYY 785

BLAST of Lsi03G016700 vs. NCBI nr
Match: XP_004136756.2 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651158.1 hypothetical protein Csa_000980 [Cucumis sativus])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 640/844 (75.83%), Postives = 707/844 (83.77%), Query Frame = 0

Query: 3   SFKLNKLQARETTFFKYIHMAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDAE 62
           SFKLN+L+ RETT+ KYI MA  I EQAENIL ELK  P YLRRM+YT+LSLK IL DAE
Sbjct: 13  SFKLNELKGRETTYSKYIRMACCIYEQAENILIELKKFPMYLRRMQYTMLSLKTILKDAE 72

Query: 63  KEEYSPYLCSWLKELQNVFTQIEGFIYEFKGKAYKQKAIGKQVFVSSLSSSQIEHTQKMK 122
           KEEY   L  WL++LQ+VF QIE  +YE   +  KQ+A GK VF+ S + SQI+ T+KM 
Sbjct: 73  KEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVFLPSFNFSQIDQTKKMM 132

Query: 123 KLCDNLDEIASQMYEFNLTNMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRF 182
           KLCD+LDEIAS MY FNLTNMETTHSFLSATEVSTRLMKPSWQLLY LTNA +VF   R+
Sbjct: 133 KLCDDLDEIASHMYGFNLTNMETTHSFLSATEVSTRLMKPSWQLLYSLTNAPKVFQDKRY 192

Query: 183 DGFLNHFKTSTHGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLI 242
             FL+HFK STHG FHIVG+ GIGK+TLAKFFYN+  +V  F SRLW+CVKEEFD QRLI
Sbjct: 193 HNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPSRLWICVKEEFDPQRLI 252

Query: 243 KEMLSFSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGM 302
           KEMLSFS+CQ TCDNLTEKQLCF VQQ+LRDKKFL+VFQDISIKNLG  NCS+F +LLGM
Sbjct: 253 KEMLSFSHCQVTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIKNLG--NCSIFKSLLGM 312

Query: 303 GNPGSKIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPE 362
           GN GSKIIVTTQNE+IADA+GL KLYKNESQVV   +ATKPS++N               
Sbjct: 313 GNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSPEATKPSDVN--------------- 372

Query: 363 IYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAI 422
             K+NM HQTIF VERLSKENSLSLFKVHAFTE QEAQ  NLTKI+EVIEQKCH VPLAI
Sbjct: 373 --KDNMKHQTIFKVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLAI 432

Query: 423 KCLGGLLSSKTSIAEKNSVIDKLWAQDEGED--MSILPTLRLCYDLMPSHLQRCFLSCSQ 482
           KCLGGLL SKTSIAE N VIDKLW  +E ED   SILPTLRLCYD MPSHLQRCFL CSQ
Sbjct: 433 KCLGGLL-SKTSIAEWNGVIDKLWEHEEEEDGNKSILPTLRLCYDQMPSHLQRCFLYCSQ 492

Query: 483 LTKDRILSSNDVIQLWIAQGLLSEENYSSLEKLGENYFKELCSRCFLQELEEYGLGYWFK 542
           L KDRILSSNDVIQLWIA  LL +ENY SLEK+GENYFKELCSRCFLQELEEYG GYWFK
Sbjct: 493 LKKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWFK 552

Query: 543 LHPLIQKLARIITQKEVFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNAN 602
           LHPLI+KLAR++TQK+VF VT+ +SIAFT+RDKVPPSAFLA  CI KF  LRLL+LGNAN
Sbjct: 553 LHPLIEKLARLLTQKQVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGNAN 612

Query: 603 LREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLI 662
           L+ IP+AV  L QLRYLDLQGNK+IKRLPNSI  L NLQTLILASCSAL+ELP+DIR+L 
Sbjct: 613 LQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQLT 672

Query: 663 HLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYDCKTL 722
           +LRYLWVTAN L LHKNGVGTMTSLRFLAIGGC NLQDLF++PSCLVRLETLMIYDCKTL
Sbjct: 673 NLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCKTL 732

Query: 723 KLLPNEIGSLISLQNLVIWSCKRLTLTLKGVEFRLRRFTIRELPKVNKLPEWLQRFTETL 782
           K LPNEIGSLISL+NLVIWSCK+LTLTLKGVEFRL+RFTIRELP V KLPEW QRFTETL
Sbjct: 733 KSLPNEIGSLISLKNLVIWSCKKLTLTLKGVEFRLQRFTIRELPIVKKLPEWTQRFTETL 792

Query: 783 RVLEIIDCPIELKDDKFKSYKALERISIHGDVRVKNSNDEYSVEFKNSVSRRQVKKEMKT 842
           RVLEIIDCPIE  DD  KSYK+LER SIHG VR KN    Y+++++N V  R+VKKE+KT
Sbjct: 793 RVLEIIDCPIEWNDDVLKSYKSLERFSIHGAVRTKNQIGGYNIDYRNFVRSRKVKKEVKT 834

Query: 843 CSYY 845
           C YY
Sbjct: 853 CVYY 834

BLAST of Lsi03G016700 vs. NCBI nr
Match: XP_016899652.1 (PREDICTED: putative disease resistance protein RGA4 [Cucumis melo])

HSP 1 Score: 947.2 bits (2447), Expect = 9.7e-272
Identity = 524/824 (63.59%), Postives = 623/824 (75.61%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNV 81
           MAY I  +AENIL ELKNCPNY RR+EYT+LSLK+ILMDA EK+E+S +L +WLKELQNV
Sbjct: 1   MAYCIYYRAENILSELKNCPNYPRRIEYTMLSLKSILMDAEEKQEHSRHLRNWLKELQNV 60

Query: 82  FTQIEGFIYEFKGKAYKQKAIGKQVFVS-SLSSSQIEHTQKMKKLCDNLDEIASQMYEFN 141
           F QIEGFI E K + YK + IGKQV    S S  Q   TQKM+KL D+LD + ++MYEF+
Sbjct: 61  FFQIEGFIDELKEEVYKTEGIGKQVLAPFSFSICQRARTQKMEKLFDHLDAVVAKMYEFD 120

Query: 142 L-------TNMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTS 201
           L         METT SFL+ATEVST LMKPSW++LYP T+A + +   R+   LN FK S
Sbjct: 121 LKERHTGAIKMETTDSFLTATEVSTSLMKPSWKVLYPSTSAPKSYQHERYREILNDFKES 180

Query: 202 THGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQ 261
           T GFFHIVG+AGIGKSTLAKF YNDP+V E F+SRLWVCVKEEFDTQRL+ E+L+FSY  
Sbjct: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEEMFTSRLWVCVKEEFDTQRLMTEILNFSYSP 240

Query: 262 ATCDNLTEKQLCFT--VQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKII 321
           ATCDNLTE + C T  VQQYLR+K FLLVFQD+SIKNL  +N S+FT+LL MG PGSKII
Sbjct: 241 ATCDNLTETKSCPTVQVQQYLREKTFLLVFQDLSIKNL--DNSSLFTSLLRMGKPGSKII 300

Query: 322 VTTQNEEIADAIGLGKLY-----------KNESQVVSFLDATKPSNINNDYVSIQANPVT 381
           VTTQNEEIA+AI L K+            +NESQ        + SN+NN   S QANP+ 
Sbjct: 301 VTTQNEEIANAIKLPKVMVEVNKVEQQSEQNESQTALDAVTIETSNVNNAGESTQANPL- 360

Query: 382 DPEIYKNNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVP 441
             +    ++T+QTIF V+RLS+E+SLSLFK +A     + +      I E +E KC+ VP
Sbjct: 361 --DKLDQSITYQTIFKVKRLSEEDSLSLFKDYASIVEDDKEG-----IIETLE-KCNGVP 420

Query: 442 LAIKCLGGLLSSKTSIAEKNSVIDKLWAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCS 501
           LAIKCLG +LS +TS  +     D+   ++  E  S    L+LCY+ MPSHL+ CFL CS
Sbjct: 421 LAIKCLGSMLSLETSATKWMEDTDQ--QEEVNESSSTFSILKLCYNQMPSHLKPCFLYCS 480

Query: 502 QLTKDRILSSNDVIQLWIAQGLL--SEENYSSLEKLGENYFKELCSRCFLQELEEYGLGY 561
           QL  D ILSSNDVIQLW+A GLL   +ENY SLE +GE YFKELCSRCFLQ++EEYGLGY
Sbjct: 481 QLQTDSILSSNDVIQLWMANGLLHSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGY 540

Query: 562 WFKLHPLIQKLARIITQKE------VFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNL 621
           WFK+HPLI+KLARI+ Q++      + PVT+  SIAF VRD+VP S+FLAE CI KF  L
Sbjct: 541 WFKMHPLIRKLARILIQEQTKDLICIKPVTKVTSIAFPVRDEVPSSSFLAEKCISKFQYL 600

Query: 622 RLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEE 681
           RLL+LG  +L+EIPN + TL  LRYLDLQGNK+IKRLPN+IC L  LQTLILASC AL+E
Sbjct: 601 RLLHLGYTDLQEIPNTIETLKHLRYLDLQGNKKIKRLPNAICNLQYLQTLILASCFALQE 660

Query: 682 LPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLET 741
           LP DI KL  LRYLWVT+N L+LHKNGVGTM SLRFLAIGGCD LQDLFEQPSCLVRLET
Sbjct: 661 LPKDIWKLSKLRYLWVTSNNLHLHKNGVGTMNSLRFLAIGGCDKLQDLFEQPSCLVRLET 720

Query: 742 LMIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLT-LKGVEFRLRRFTIRELPKVNKLP 801
           LMIY+CK+L+LLPNE+GSLISLQNLVIWSC++LTL  L+ V+F L+RFTIRELPKVNKLP
Sbjct: 721 LMIYNCKSLQLLPNEMGSLISLQNLVIWSCEQLTLKGLEKVDFSLQRFTIRELPKVNKLP 780

Query: 802 EWLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRV 815
           EWLQR TETLRVLEIIDCPI ++++  K YKA+E   I G V V
Sbjct: 781 EWLQRSTETLRVLEIIDCPIRVEEEGIKMYKAVESKIIQGAVDV 811

BLAST of Lsi03G016700 vs. NCBI nr
Match: XP_004136757.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN59374.1 hypothetical protein Csa_002388 [Cucumis sativus])

HSP 1 Score: 938.3 bits (2424), Expect = 4.5e-269
Identity = 520/823 (63.18%), Postives = 622/823 (75.58%), Query Frame = 0

Query: 22  MAYRIDEQAENILRELKNCPNYLRRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQNV 81
           MAY I  +AENIL ELKN PNY RR+EYT+LSLK+ILMDA EK+E S  L +WL+ELQNV
Sbjct: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60

Query: 82  FTQIEGFIYEFKGKAYKQKAIGKQVFVS-SLSSSQIEHTQKMKKLCDNLDEIASQMYEFN 141
           F+QIEGFI E K +AY  + IGKQV    S SS+QI  T KM+KL D+L+E+A++MYEFN
Sbjct: 61  FSQIEGFIDEHKEEAY--EGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120

Query: 142 LT-------NMETTHSFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTS 201
           LT         ETT+SFL+ATEVSTRLMKPSW++LYPLTNA + +   R+   LN FK  
Sbjct: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180

Query: 202 THGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQ 261
           T GFFHIVG+AGIGKSTLAKF YNDP+V   F SRLWVCVKEEFDTQRL+KE+L+FSY  
Sbjct: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240

Query: 262 ATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVT 321
           ATCDNLT K LC T  QYLR++ FLLVFQD+SIKNL  +NCS+FT+LL MG PGSKIIVT
Sbjct: 241 ATCDNLTTK-LCPT-DQYLRERTFLLVFQDLSIKNL--DNCSLFTSLLMMGKPGSKIIVT 300

Query: 322 TQNEEIADAIGLGKLYK-------NESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYK 381
           TQNEEIA+AI L  +YK       N SQ        + +N+NN    +QANP+   +   
Sbjct: 301 TQNEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKID--- 360

Query: 382 NNMTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCL 441
            ++  QTIF V+RLS+++SLSLFK +A T   E    ++ K      +KC+ +PLAIKCL
Sbjct: 361 QSIPSQTIFKVKRLSEKDSLSLFKDYAST--YEGNEKDIMK----TLKKCNGIPLAIKCL 420

Query: 442 GGLLSSKTSIAEKNSVIDKLWAQD------EGEDMSILPTLRLCYDLMPSHLQRCFLSCS 501
           G +LS      +        W +D      + E  S    L+LCY+ MPSHL+RCFL CS
Sbjct: 421 GSMLSLGPPATK--------WMEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCS 480

Query: 502 QLTKDRILSSNDVIQLWIAQGLL--SEENYSSLEKLGENYFKELCSRCFLQELEEYGLGY 561
           QL  D ILSSNDVIQLW+A GLL   +ENY SLE +GE YFKELCSRCFLQ++EEYGLGY
Sbjct: 481 QLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGY 540

Query: 562 WFKLHPLIQKLARIITQK-----EVFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLR 621
           WFK+HPLI++LAR++ ++      + PVT   SIAF VRD+VP S+FLAE CI KF +LR
Sbjct: 541 WFKMHPLIRELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLR 600

Query: 622 LLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEEL 681
           LLYLG+ +L+EIPN + TL  L YLDLQGNK IKRLPN+IC L +LQTLILASCSALEEL
Sbjct: 601 LLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEEL 660

Query: 682 PDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETL 741
           P DI KL +LRYLWVT+NKL LHKNGVGTMTSLRFLAIGGCD LQDLFE+PSCLVRLETL
Sbjct: 661 PKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETL 720

Query: 742 MIYDCKTLKLLPNEIGSLISLQNLVIWSCKRLTLT-LKGVEFRLRRFTIRELPKVNKLPE 801
           MIYDC +L+LLPNE+GSLISLQNLVIWSCK+LTL  L+ V+F L+RFTIRELP+VNKLPE
Sbjct: 721 MIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPE 780

Query: 802 WLQRFTETLRVLEIIDCPIELKDDKFKSYKALERISIHGDVRV 815
           WLQR TETLRVLEIIDCPI+++++  K YKA+E   I G V +
Sbjct: 781 WLQRSTETLRVLEIIDCPIKVEEEGIKMYKAVESKIIQGAVDI 800

BLAST of Lsi03G016700 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 187.6 bits (475), Expect = 4.2e-47
Identity = 177/707 (25.04%), Postives = 302/707 (42.72%), Query Frame = 0

Query: 35  RELKNCPNYLRRMEYTILSLKAILMDAEKEEY-SPYLCSWLKELQNVFTQIEGFIYEFKG 94
           REL    N L R+   +L++ A+L+DAE+++  +P +  W+ EL++V    E  + +   
Sbjct: 32  RELNE--NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDI-- 91

Query: 95  KAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLD------EIASQMYEFNLTNMETTH 154
                +A+   +   S SS+++   +    L D LD      E   +     L  + +  
Sbjct: 92  ---ATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQR 151

Query: 155 SFLSATEVSTRLMKPSWQLLYPLTNARQVFHGSRFDGFLNHFKTSTHG------FFHIVG 214
           + L   E++  + K        L +  +VF        +  F    +G         IVG
Sbjct: 152 NILGLKELTAMIPKQRLPTT-SLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVG 211

Query: 215 DAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLTEK 274
             G+GK+TL++  YND  V   F +++W  V EEFD  ++ K++          +++T +
Sbjct: 212 IGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVY---------ESVTSR 271

Query: 275 QLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIVTTQNEEIADA 334
              FT                    +L +    +   L G G P   ++    NE  AD 
Sbjct: 272 PCEFT--------------------DLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 331

Query: 335 IGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNVERLSK 394
             L + + + +Q    L  T+   + +   ++                   + N++ LS 
Sbjct: 332 DLLRQPFIHAAQGSQILVTTRSQRVASIMCAVH------------------VHNLQPLSD 391

Query: 395 ENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSIAEKNSV 454
            +  SLF    F   +   +  +  + E I  KC  +PLA+K LGG+L  +  + E   V
Sbjct: 392 GDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERV 451

Query: 455 IDKLWAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSNDVIQLWIAQGL 514
           +         +  ++LP LR+ Y  +P+HL+RCF  CS   K      + V+ LW+A+G 
Sbjct: 452 LSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGF 511

Query: 515 LSEENYS-SLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLIQKLARIITQKE---- 574
           L +   S +LE+LG  YF EL SR  LQ+ +       + +H  I +LA+  + +     
Sbjct: 512 LQQTRSSKNLEELGNEYFSELESRSLLQKTKTR-----YIMHDFINELAQFASGEFSSKF 571

Query: 575 ----VFPVTEPKSIAFTVRDK-VPPSAF-----------------------------LAE 634
                  V+E       +RD    P  F                             ++E
Sbjct: 572 EDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQMVSE 631

Query: 635 TCIKKFNNLRLLYLGNANLREI-PNAVGTLTQLRYLDLQGNKRIKRLPNSICYLPNLQTL 689
             +     LR+L L +  +  + P+    ++  R+LDL     +++LP S+CY+ NLQTL
Sbjct: 632 KLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEKLPKSLCYMYNLQTL 677

BLAST of Lsi03G016700 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 150.2 bits (378), Expect = 7.4e-36
Identity = 162/722 (22.44%), Postives = 298/722 (41.27%), Query Frame = 0

Query: 22  MAYRIDEQAENI-LRELKNCPNYLRRMEYTILSLKAILMDA-EKEEYSPYLCSWLKELQN 81
           M  RI+   E + L + K+    L+R++  +++   +L DA ++ E+   +  WL  +++
Sbjct: 13  MVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKD 72

Query: 82  VFTQIEGFIYEFKGKAYKQKAIGKQVFVSSLSSSQIEHTQKMKKLCDNLDEIASQMYEFN 141
            F Q E  + E + +A +++ + +   +  L  + +   + ++K  +   E   ++ E +
Sbjct: 73  AFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHH 132

Query: 142 LTNMETTHSFLSATEVSTRLMKPSWQLLY-----PLTNARQVFHGSRFDGFLN------H 201
           + ++E     +   E S    +P W+         L   R V         +N       
Sbjct: 133 VKHIEV----IGLKEYS-ETREPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDE 192

Query: 202 FKTSTHGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEML-S 261
                     +VG  G+GK+TL +  +ND +V E+F  ++W+     F+   + K +L  
Sbjct: 193 ISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQD 252

Query: 262 FSYCQATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGS 321
            +      ++L   Q+   +++ L  K+FLLV  D   ++   +    F         GS
Sbjct: 253 ITSSAVNTEDLPSLQI--QLKKTLSGKRFLLVLDDFWSES--DSEWESFQVAFTDAEEGS 312

Query: 322 KIIVTTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNN 381
           KI++TT++E ++      K+Y+                                      
Sbjct: 313 KIVLTTRSEIVSTVAKAEKIYQ-------------------------------------- 372

Query: 382 MTHQTIFNVERLSKENSLSLFKVHAFTEAQEAQ-SSNLTKIEEVIEQKCHRVPLAIKCLG 441
                   ++ ++ E    L    AF        +  L  I + I ++C  +PLA + + 
Sbjct: 373 --------MKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIA 432

Query: 442 GLLSSKTSIAEKNSVIDKLWAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRI 501
             L SK +  +  +V     +       SILP L+L YD +P  L+RCF  CS   K  +
Sbjct: 433 SHLRSKPNPDDWYAVSKNFSSYTN----SILPVLKLSYDSLPPQLKRCFALCSIFPKGHV 492

Query: 502 LSSNDVIQLWIAQGLLSEENYS-SLEKLGENYFKELCSRCFLQELEEYGLGYWFKLHPLI 561
               +++ LW+A  LL +   S  LE +G +Y  +L ++ F Q L+       F +H L+
Sbjct: 493 FDREELVLLWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDITMTS--FVMHDLM 552

Query: 562 QKLARIITQKEVFPVTEPK--------------------SIAF----------------- 621
             LA+ ++    F + +                      S+AF                 
Sbjct: 553 NDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNS 612

Query: 622 -TVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNKRIKR 681
            T  + +  +  +    +   + LR+L L +  +  +P ++  L  LRYLDL   K IK 
Sbjct: 613 PTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTK-IKE 672

Query: 682 LPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRYLWVTANKLYLHKNGVGTMTSLRF 690
           LP  +C L NLQTL+L++C  L  LP  I +LI+LR L +    L     G+  + SL+ 
Sbjct: 673 LPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQK 672

BLAST of Lsi03G016700 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 136.7 bits (343), Expect = 8.5e-32
Identity = 145/557 (26.03%), Postives = 249/557 (44.70%), Query Frame = 0

Query: 200 VGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLT 259
           VG  G+GK+T+A+  +ND ++   F  R+WV V + F  +++++ +L      +  D++ 
Sbjct: 188 VGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDI- 247

Query: 260 EKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNP---GSKIIVTTQNE 319
              L   +QQYL  K++L+V  D+  KNL       + + +  G P   G  +IVTT++E
Sbjct: 248 -GTLLRKIQQYLLGKRYLIVMDDVWDKNLS------WWDKIYQGLPRGQGGSVIVTTRSE 307

Query: 320 EIADAIGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNV 379
            +A                                 +QA         +++ TH+     
Sbjct: 308 SVAK-------------------------------RVQA---------RDDKTHRP---- 367

Query: 380 ERLSKENSLSLFKVHAFTEAQ-EAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSI 439
           E LS +NS  LF   AF       +   L  + + I  KC  +PL IK +GGLL  K  +
Sbjct: 368 ELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHV 427

Query: 440 AEKNSVIDKLWAQDE-----GEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSN 499
             +   I + + QDE      E  +++ +L+L YD +PSHL+ C L+ S   +D ++   
Sbjct: 428 YHEWRRIAEHF-QDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQ 487

Query: 500 DVIQLWIAQGLLSEENYSSLEKLGENYFKELCSRCFLQELEE--YGLGYWFKLHPLIQKL 559
            ++  WI +G +   N  S  + GE+ F  L +RC ++ +++   G     K+H +++ L
Sbjct: 488 QLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDL 547

Query: 560 ARIITQKEVFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAV 619
              I +K+ F  + P+ +       +  S    E  IK  + LR + +      E+    
Sbjct: 548 VIDIAKKDSF--SNPEGLNCR---HLGISGNFDEKQIKVNHKLRGV-VSTTKTGEVNKLN 607

Query: 620 GTL----TQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRY 679
             L    T  +YL      R+  +  SI   P            L E+ D+I  L HL  
Sbjct: 608 SDLAKKFTDCKYL------RVLDISKSIFDAP------------LSEILDEIASLQHLAC 664

Query: 680 LWVT-ANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYD---CKTL 738
           L ++  + L      +  + +L+ L    C NL+ L  QP C+V  + L++ D   C +L
Sbjct: 668 LSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQL--QP-CIVLFKKLLVLDMTNCGSL 664

BLAST of Lsi03G016700 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 136.7 bits (343), Expect = 8.5e-32
Identity = 145/557 (26.03%), Postives = 249/557 (44.70%), Query Frame = 0

Query: 200 VGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYCQATCDNLT 259
           VG  G+GK+T+A+  +ND ++   F  R+WV V + F  +++++ +L      +  D++ 
Sbjct: 188 VGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDI- 247

Query: 260 EKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNP---GSKIIVTTQNE 319
              L   +QQYL  K++L+V  D+  KNL       + + +  G P   G  +IVTT++E
Sbjct: 248 -GTLLRKIQQYLLGKRYLIVMDDVWDKNLS------WWDKIYQGLPRGQGGSVIVTTRSE 307

Query: 320 EIADAIGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQTIFNV 379
            +A                                 +QA         +++ TH+     
Sbjct: 308 SVAK-------------------------------RVQA---------RDDKTHRP---- 367

Query: 380 ERLSKENSLSLFKVHAFTEAQ-EAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSSKTSI 439
           E LS +NS  LF   AF       +   L  + + I  KC  +PL IK +GGLL  K  +
Sbjct: 368 ELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHV 427

Query: 440 AEKNSVIDKLWAQDE-----GEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTKDRILSSN 499
             +   I + + QDE      E  +++ +L+L YD +PSHL+ C L+ S   +D ++   
Sbjct: 428 YHEWRRIAEHF-QDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQ 487

Query: 500 DVIQLWIAQGLLSEENYSSLEKLGENYFKELCSRCFLQELEE--YGLGYWFKLHPLIQKL 559
            ++  WI +G +   N  S  + GE+ F  L +RC ++ +++   G     K+H +++ L
Sbjct: 488 QLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDL 547

Query: 560 ARIITQKEVFPVTEPKSIAFTVRDKVPPSAFLAETCIKKFNNLRLLYLGNANLREIPNAV 619
              I +K+ F  + P+ +       +  S    E  IK  + LR + +      E+    
Sbjct: 548 VIDIAKKDSF--SNPEGLNCR---HLGISGNFDEKQIKVNHKLRGV-VSTTKTGEVNKLN 607

Query: 620 GTL----TQLRYLDLQGNKRIKRLPNSICYLPNLQTLILASCSALEELPDDIRKLIHLRY 679
             L    T  +YL      R+  +  SI   P            L E+ D+I  L HL  
Sbjct: 608 SDLAKKFTDCKYL------RVLDISKSIFDAP------------LSEILDEIASLQHLAC 664

Query: 680 LWVT-ANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQPSCLVRLETLMIYD---CKTL 738
           L ++  + L      +  + +L+ L    C NL+ L  QP C+V  + L++ D   C +L
Sbjct: 668 LSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQL--QP-CIVLFKKLLVLDMTNCGSL 664

BLAST of Lsi03G016700 vs. TAIR 10
Match: AT1G59780.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 110.2 bits (274), Expect = 8.5e-24
Identity = 137/556 (24.64%), Postives = 246/556 (44.24%), Query Frame = 0

Query: 192 STHGFFHIVGDAGIGKSTLAKFFYNDPKVVENFSSRLWVCVKEEFDTQRLIKEMLSFSYC 251
           S+HG   I G  G+GK+TLA+  ++  KV  +F    WVCV +EF  + + K +L     
Sbjct: 179 SSHG-VSITGLGGLGKTTLARQIFDHDKVKSHFDGLAWVCVSQEFTRKDVWKTILGNLSP 238

Query: 252 QATCDNLTEKQLCFTVQQYLRDKKFLLVFQDISIKNLGINNCSMFTNLLGMGNPGSKIIV 311
           +    +L E  +   + Q L  KK L+VF D+  +        MF         G K+++
Sbjct: 239 KYKDSDLPEDDIQKKLFQLLETKKALIVFDDLWKREDWYRIAPMFPE----RKAGWKVLL 298

Query: 312 TTQNEEIADAIGLGKLYKNESQVVSFLDATKPSNINNDYVSIQANPVTDPEIYKNNMTHQ 371
           T++N+ I                V+F    KP  + +D            E +K      
Sbjct: 299 TSRNDAI------------HPHCVTF----KPELLTHD------------ECWK------ 358

Query: 372 TIFNVERLSKENSLSLFKVHAFTEAQEAQSSNLTKIEEVIEQKCHRVPLAIKCLGGLLSS 431
            +      SK+ +++ + +             + K+ + + + C R+PLA+K LGGLL +
Sbjct: 359 -LLQRIAFSKQKTITGYII----------DKEMVKMAKEMTKHCKRLPLAVKLLGGLLDA 418

Query: 432 KTSIAEKNSVIDKL--------WAQDEGEDMSILPTLRLCYDLMPSHLQRCFLSCSQLTK 491
           K ++ +   + + +         + +E +  S+   L L ++ +P +L+ C L  +   +
Sbjct: 419 KHTLRQWKLISENIISHIVVGGTSSNENDSSSVNHVLSLSFEGLPGYLKHCLLYLASYPE 478

Query: 492 DRILSSNDVIQLWIAQGLLSEENY--SSLEKLGENYFKELCSRCFLQELEEYGLGYWF-- 551
           D  +    +  +W A+G+    NY  +++  + + Y +EL  R  +   E   L   F  
Sbjct: 479 DHEIEIERLSYVWAAEGITYPGNYEGATIRDVADLYIEELVKRNMVIS-ERDALTSRFEK 538

Query: 552 -KLHPLIQKLARIITQKEVF--PVTEPKSIA-----FTVRDK----VPPSAFLAETCIK- 611
            +LH L++++  +  ++E F   VT+P S +      + R +       S F  E  +K 
Sbjct: 539 CQLHDLMREICLLKAKEENFLQIVTDPTSSSSVHSLASSRSRRLVVYNTSIFSGENDMKN 598

Query: 612 -KFNNLRLLYLGNANLREIPNAVGTLTQLRYLDLQGNK-RIKRLPNSICYLPNLQTLILA 671
            K  +L  + +G +      N +  L  LR LDL G K +  +LP+SI  L +L+ L L 
Sbjct: 599 SKLRSLLFIPVGYSRFSMGSNFI-ELPLLRVLDLDGAKFKGGKLPSSIGKLIHLKYLSLY 658

Query: 672 SCSALEELPDDIRKLIHLRY--LWVTANKLYLHKNGVGTMTSLRFLAIGGCDNLQDLFEQ 719
             S +  LP  +R L  L Y  L + + +L    N    M  LR+L++    +     E 
Sbjct: 659 QAS-VTYLPSSLRNLKSLLYLNLRINSGQLINVPNVFKEMLELRYLSLPWERSSLTKLEL 680