Lsi03G016340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G016340
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein NRT1/ PTR FAMILY 3.1-like
Locationchr03: 27463003 .. 27465022 (-)
RNA-Seq ExpressionLsi03G016340
SyntenyLsi03G016340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATGAAGTGTGCGATAGATTTGCAAGCTCAGGGTTCCATGCCAACATAATAACATATTTGACACAAGATCTTAACATGCCTCTTGTTTCTGCCTCCAACATTCTCACCAACTTCTCTGCAACTTCCAGCTTCACTTCTCTCATCGGCGCTCTCATTGCCGATTCCTTCGCCGGCCGCTTTTGGACCATCACCTTTGCCTCCATCATCTACGAGCTCGTACGATAATCAGACCTAATTTTATTTTTATAACATACCCAAGTTACTTTTTTCATTGTTAATTACTTCTGATTCTTGCATGTTATTACAGGGAATGATCACTATCACAATTTCCGCCATACTCCCATCGCTCCACCCGCCACCCTGTCCCACCCAACTCAACTGCACGCAAGCCTCCGGCACACAATTAATGTTCCTCTACCTCGCTCTCCTCCTCACATCCCTCGGCGCAGGGGGGATTCGGCCATGTGTCGTTGCATTCGCGGCCGACCAATTCGACATGACGAAGGTCGGTGTGGCGGGGCGGACGTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGAATGGCTACTCTTACAGCTCTTACGGTGGTGGTGTATATTCAAGACAACGTGGGGTGGGGGTGGGGGTTCGGCATTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCGTTTGTTGTGGGCTCGCCCCTTTACAACAAACTCAAGCCGAGTGGTAGCCCTTTGGTTCGATTGGCTCAGGTTGTGGTTGCAGCTTTTAAGAACCGAAAGGCTGTTTTGCCTCATGACTCCAACCTTTTGTATCGGAATCATAAGCTTGATGCTGCCATTGCCATTCAAGGAAGACTTGTCCACACCGATCAGTTCAAGTAAGCCTAAATTTTTTATTGTGCTGTTTTTAAATATAGAAAAATGATATTTTTAGATAATAATTGGTGTCTTGTTTATCGAACTATATTTGAAATTGTTGAATTATAATAAAAGAAAAGTACATGTGATGCATTGAATACAATTAACATAAACATTAAATTAAAAGAATCGATGGCGTAACTTACAAAATAAAAATTTGATAATTTAAGCGTTGTTTGATAATTATTTTATTTTTTGATTATGGAAGTTAATTAAGTTTATAAGCGCTAGTTTCTTTATAGTTTTTTATTTTATTACTTATTTTTTTAAAATATTTTAAAAAACCAATTGAATATTTTTGAAAACTAAAAAAAAAAAAAAGTAATTTTGAAAGACTTAAATTTGTTTTTGAAATTTGGCTTAGAATTAAAAGGTTTAAAAGGTTTTTTTAAGGAATTTGAAAAGTCTACTCAAACCTTAGGATAAAACAAACATAATTTGCAAAAACTTAATTAAAAAAAAGATATAATAGTTATCAAATGAAGTCTTTTAATATATATTATCAAATCGTAGAAAGCTTAATGATTTTTTTTTTTTTTTTGAAAGGAGTAGATGGCTTAATGATTCAATTTAATTATTGTTGAATTAAAAATAAACATATATTACATTTTTTTTAATGCGGGAACTAAACCAGCATAAATCCTTTTTTTGGAAAAATATAACTTTTTAAAAAAATTTAGAGTCTAGTTTCCATTTTGTCTTTAAGTTTCAAAATGTTACACTTTTACAATTGAATTTTGAGTTTAATTTTATTTGGTCCCTAAGTTTTAAGATTTACCTTATTAACTTTGATTTTTCACTAATATTCATTTTCTGTCATGTGATGAGAACAAACCTAAAACTTAAAAGATAAGAATAGAAACCGTGTAACTTATAATCTCATATATATTTTTTTTACAATATTTGAGGTTGAGAGGATCAACCTCTTATCTCAAATTCGACCGTATAAGTTTTATGTCAGTTGAACTATGTTCAATAATTTATATGTTTGCAATATTTTCAGGTGGCTGGACAAGGCCGCTGTGACAACAACTCCAAACTTAATAACAGATCCACCGAATCTGTGGCGGCTGGCCACCGTACACAGAGTCGAAGAGCTAAAATCCATCAT

mRNA sequence

GAAATGAAGTGTGCGATAGATTTGCAAGCTCAGGGTTCCATGCCAACATAATAACATATTTGACACAAGATCTTAACATGCCTCTTGTTTCTGCCTCCAACATTCTCACCAACTTCTCTGCAACTTCCAGCTTCACTTCTCTCATCGGCGCTCTCATTGCCGATTCCTTCGCCGGCCGCTTTTGGACCATCACCTTTGCCTCCATCATCTACGAGCTCGGAATGATCACTATCACAATTTCCGCCATACTCCCATCGCTCCACCCGCCACCCTGTCCCACCCAACTCAACTGCACGCAAGCCTCCGGCACACAATTAATGTTCCTCTACCTCGCTCTCCTCCTCACATCCCTCGGCGCAGGGGGGATTCGGCCATGTGTCGTTGCATTCGCGGCCGACCAATTCGACATGACGAAGGTCGGTGTGGCGGGGCGGACGTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGAATGGCTACTCTTACAGCTCTTACGGTGGTGGTGTATATTCAAGACAACGTGGGGTGGGGGTGGGGGTTCGGCATTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCGTTTGTTGTGGGCTCGCCCCTTTACAACAAACTCAAGCCGAGTGGTAGCCCTTTGGTTCGATTGGCTCAGGTTGTGGTTGCAGCTTTTAAGAACCGAAAGGCTGTTTTGCCTCATGACTCCAACCTTTTGTATCGGAATCATAAGCTTGATGCTGCCATTGCCATTCAAGGAAGACTTGTCCACACCGATCAGTTCAAATCCACCGAATCTGTGGCGGCTGGCCACCGTACACAGAGTCGAAGAGCTAAAATCCATCAT

Coding sequence (CDS)

ATGCCTCTTGTTTCTGCCTCCAACATTCTCACCAACTTCTCTGCAACTTCCAGCTTCACTTCTCTCATCGGCGCTCTCATTGCCGATTCCTTCGCCGGCCGCTTTTGGACCATCACCTTTGCCTCCATCATCTACGAGCTCGGAATGATCACTATCACAATTTCCGCCATACTCCCATCGCTCCACCCGCCACCCTGTCCCACCCAACTCAACTGCACGCAAGCCTCCGGCACACAATTAATGTTCCTCTACCTCGCTCTCCTCCTCACATCCCTCGGCGCAGGGGGGATTCGGCCATGTGTCGTTGCATTCGCGGCCGACCAATTCGACATGACGAAGGTCGGTGTGGCGGGGCGGACGTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGAATGGCTACTCTTACAGCTCTTACGGTGGTGGTGTATATTCAAGACAACGTGGGGTGGGGGTGGGGGTTCGGCATTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCGTTTGTTGTGGGCTCGCCCCTTTACAACAAACTCAAGCCGAGTGGTAGCCCTTTGGTTCGATTGGCTCAGGTTGTGGTTGCAGCTTTTAAGAACCGAAAGGCTGTTTTGCCTCATGACTCCAACCTTTTGTATCGGAATCATAAGCTTGATGCTGCCATTGCCATTCAAGGAAGACTTGTCCACACCGATCAGTTCAAATCCACCGAATCTGTGGCGGCTGGCCACCGTACACAGAGTCGAAGAGCTAAAATCCATCAT

Protein sequence

MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAAGHRTQSRRAKIHH
Homology
BLAST of Lsi03G016340 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-76
Identity = 144/240 (60.00%), Postives = 175/240 (72.92%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           +PL  A+N LTNF+ TSS T L+GA IADSFAGRFWTITFASIIY++GM  +TISAI+P+
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           L PPPC  +  C  A   QL  LY+ALLL +LG+GGIRPCVVAF ADQFD +      +T
Sbjct: 121 LRPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKT 180

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WN+FNWYYFCMG A L A+TV+V+IQDNVGWG G GIPT+AM LSV+AFV G  LY  L 
Sbjct: 181 WNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLV 240

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           P+GSP  RL QV VAAF+ RK  +  D +LLY N ++DA I++ G+L HT      +  A
Sbjct: 241 PAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAA 300

BLAST of Lsi03G016340 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 8.5e-49
Identity = 103/243 (42.39%), Postives = 146/243 (60.08%), Query Frame = 0

Query: 5   SASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPP 64
           +A+N +TN+S T   T LIGA IAD++ GR+WTI     IY  GM  +T+SA +P L P 
Sbjct: 66  TAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPG 125

Query: 65  PCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFF 124
            C    +    + +Q    ++AL + +LG GGI+PCV +F ADQFD        +  +FF
Sbjct: 126 NC--NADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 185

Query: 125 NWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGS 184
           NW+YF + +  L A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GS
Sbjct: 186 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 245

Query: 185 PLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAAGHR 244
           PL R+ QV+VAAF+     +P D +LL+     ++ I    +LVHTD  K  +  A   +
Sbjct: 246 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 305

Query: 245 TQS 248
           + S
Sbjct: 306 SDS 306

BLAST of Lsi03G016340 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-48
Identity = 106/230 (46.09%), Postives = 144/230 (62.61%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           M  VSAS  ++N+S T   T LIGA IAD++ GR+WTI    +IY  GM  +TISA +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           L  P C  +  C   +G Q    ++AL L +LG GGI+PCV +F ADQFD T        
Sbjct: 123 L-TPTCSGE-TCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 182

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
            +FFNW+YF + +  + A +V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   K
Sbjct: 183 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 242

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHT 231
           P GSPL R+ QV+VA+ +  K  +P D +LLY N   +++I    +L HT
Sbjct: 243 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHT 289

BLAST of Lsi03G016340 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.2e-48
Identity = 102/245 (41.63%), Postives = 150/245 (61.22%), Query Frame = 0

Query: 4   VSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHP 63
           VSA+  +T +  T   T LIGA++AD++ GR+WTI   S IY +GM  +T+SA +P+L P
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 64  PPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNF 123
             C     C  A+  Q    +  L L +LG GGI+PCV +F ADQFD T      R  +F
Sbjct: 142 AECIGDF-CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 201

Query: 124 FNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSG 183
           FNW+YF + +  L + +++V+IQ+N GWG GFGIPT+ M L++ +F  G+PLY   KP G
Sbjct: 202 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 261

Query: 184 SPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAAGH 243
           SP+ R++QVVVA+F+     +P D+ LLY     ++AIA   ++ HTD  +  +  A   
Sbjct: 262 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 321

Query: 244 RTQSR 249
             +S+
Sbjct: 322 EEESK 325

BLAST of Lsi03G016340 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.5e-48
Identity = 103/247 (41.70%), Postives = 151/247 (61.13%), Query Frame = 0

Query: 4   VSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHP 63
           VSA++ +  +  T   T LIGA+IADS+ GR+WTI   S IY +GM  +T+SA LP L P
Sbjct: 93  VSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKP 152

Query: 64  PPCP--TQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTW 123
             C       C+ A+  Q    +  L L +LG GGI+PCV +F ADQFD T      R  
Sbjct: 153 AACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKA 212

Query: 124 NFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKP 183
           +FFNW+YF + + +  + T++V++Q+NVGWG GF IPT+ M +S+ +F +G+PLY   KP
Sbjct: 213 SFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKP 272

Query: 184 SGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAA 243
            GSP+ R+ QV+VAA++  K  LP D + LY   + ++ IA   ++ HTD +K  +  A 
Sbjct: 273 GGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAV 332

Query: 244 GHRTQSR 249
               +S+
Sbjct: 333 ISEYESK 339

BLAST of Lsi03G016340 vs. ExPASy TrEMBL
Match: Q96400 (Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 1.0e-121
Identity = 224/240 (93.33%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 73  MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 132

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NCTQASGTQLM LYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 133 LHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 192

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 193 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 252

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIAIQGRLVHTDQFK  +  A
Sbjct: 253 PSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQGRLVHTDQFKWLDKAA 312

BLAST of Lsi03G016340 vs. ExPASy TrEMBL
Match: A0A5A7UI29 (Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006470 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 222/240 (92.50%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 1   MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 60

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NC QASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 61  LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 120

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 180

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIA+QG+LVHTDQFK  +  A
Sbjct: 181 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 240

BLAST of Lsi03G016340 vs. ExPASy TrEMBL
Match: A0A1S4DUU1 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 222/240 (92.50%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 40  MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 99

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NC QASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 100 LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 159

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 160 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 219

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIA+QG+LVHTDQFK  +  A
Sbjct: 220 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 279

BLAST of Lsi03G016340 vs. ExPASy TrEMBL
Match: A0A6J1I1C5 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.4e-116
Identity = 216/240 (90.00%), Postives = 222/240 (92.50%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM TITISAI+P 
Sbjct: 71  MPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGMATITISAIIPR 130

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQLNC QAS TQL   YLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT
Sbjct: 131 LHPPPCPTQLNCIQASSTQLTVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 190

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 191 WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 250

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAA K RKA LP D  LLYRNHKLDAAIAIQGRLVHTDQFK  +  A
Sbjct: 251 PSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTDQFKWLDKAA 310

BLAST of Lsi03G016340 vs. ExPASy TrEMBL
Match: A0A6J1FLW3 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 4.1e-115
Identity = 215/240 (89.58%), Postives = 222/240 (92.50%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM TITISAI+P 
Sbjct: 72  MPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGMATITISAIIPR 131

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQLNC QAS TQL  LYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT
Sbjct: 132 LHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 191

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 192 WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 251

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVV A K RKA LP D  LLYRNHKLDAAIAIQGRLVHT+QFK  +  A
Sbjct: 252 PSGSPLVRLAQVVVTAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTNQFKWLDKAA 311

BLAST of Lsi03G016340 vs. NCBI nr
Match: NP_001267552.1 (probable nitrite transporter At1g68570-like [Cucumis sativus] >KGN59416.1 hypothetical protein Csa_000876 [Cucumis sativus] >CAA93316.2 nitrite transporter [Cucumis sativus])

HSP 1 Score: 446.0 bits (1146), Expect = 2.1e-121
Identity = 224/240 (93.33%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 73  MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 132

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NCTQASGTQLM LYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 133 LHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 192

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 193 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 252

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIAIQGRLVHTDQFK  +  A
Sbjct: 253 PSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQGRLVHTDQFKWLDKAA 312

BLAST of Lsi03G016340 vs. NCBI nr
Match: XP_016899751.1 (PREDICTED: protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo])

HSP 1 Score: 444.5 bits (1142), Expect = 6.1e-121
Identity = 222/240 (92.50%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 40  MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 99

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NC QASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 100 LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 159

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 160 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 219

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIA+QG+LVHTDQFK  +  A
Sbjct: 220 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 279

BLAST of Lsi03G016340 vs. NCBI nr
Match: KAA0053906.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo var. makuwa] >TYK25499.1 protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 444.5 bits (1142), Expect = 6.1e-121
Identity = 222/240 (92.50%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 1   MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 60

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQ+NC QASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 61  LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 120

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 180

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LLYRNH+LDAAIA+QG+LVHTDQFK  +  A
Sbjct: 181 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 240

BLAST of Lsi03G016340 vs. NCBI nr
Match: XP_038903972.1 (protein NRT1/ PTR FAMILY 3.1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 436.8 bits (1122), Expect = 1.3e-118
Identity = 219/240 (91.25%), Postives = 227/240 (94.58%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSF GRFWTIT ASIIYELGM TITISAILPS
Sbjct: 30  MPLVPASNILTNFAATSSFTSLIGALIADSFTGRFWTITVASIIYELGMATITISAILPS 89

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQLNCTQASG QLM LYL+LLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 90  LHPPPCPTQLNCTQASGMQLMVLYLSLLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 149

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 150 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 209

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           P GSPLVRLAQVVVAA KNRKA+LP+DS LLYRNHKLDAAIA+QGRLVHTDQFK  +  A
Sbjct: 210 PGGSPLVRLAQVVVAAVKNRKAILPNDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAA 269

BLAST of Lsi03G016340 vs. NCBI nr
Match: XP_038903971.1 (protein NRT1/ PTR FAMILY 3.1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 436.8 bits (1122), Expect = 1.3e-118
Identity = 219/240 (91.25%), Postives = 227/240 (94.58%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           MPLV ASNILTNF+ATSSFTSLIGALIADSF GRFWTIT ASIIYELGM TITISAILPS
Sbjct: 69  MPLVPASNILTNFAATSSFTSLIGALIADSFTGRFWTITVASIIYELGMATITISAILPS 128

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           LHPPPCPTQLNCTQASG QLM LYL+LLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 129 LHPPPCPTQLNCTQASGMQLMVLYLSLLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 188

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 189 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 248

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           P GSPLVRLAQVVVAA KNRKA+LP+DS LLYRNHKLDAAIA+QGRLVHTDQFK  +  A
Sbjct: 249 PGGSPLVRLAQVVVAAVKNRKAILPNDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAA 308

BLAST of Lsi03G016340 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 2.6e-77
Identity = 144/240 (60.00%), Postives = 175/240 (72.92%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           +PL  A+N LTNF+ TSS T L+GA IADSFAGRFWTITFASIIY++GM  +TISAI+P+
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           L PPPC  +  C  A   QL  LY+ALLL +LG+GGIRPCVVAF ADQFD +      +T
Sbjct: 121 LRPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKT 180

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
           WN+FNWYYFCMG A L A+TV+V+IQDNVGWG G GIPT+AM LSV+AFV G  LY  L 
Sbjct: 181 WNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLV 240

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVA 240
           P+GSP  RL QV VAAF+ RK  +  D +LLY N ++DA I++ G+L HT      +  A
Sbjct: 241 PAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAA 300

BLAST of Lsi03G016340 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 195.3 bits (495), Expect = 6.0e-50
Identity = 103/243 (42.39%), Postives = 146/243 (60.08%), Query Frame = 0

Query: 5   SASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPP 64
           +A+N +TN+S T   T LIGA IAD++ GR+WTI     IY  GM  +T+SA +P L P 
Sbjct: 66  TAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPG 125

Query: 65  PCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFF 124
            C    +    + +Q    ++AL + +LG GGI+PCV +F ADQFD        +  +FF
Sbjct: 126 NC--NADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 185

Query: 125 NWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGS 184
           NW+YF + +  L A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GS
Sbjct: 186 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 245

Query: 185 PLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAAGHR 244
           PL R+ QV+VAAF+     +P D +LL+     ++ I    +LVHTD  K  +  A   +
Sbjct: 246 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 305

Query: 245 TQS 248
           + S
Sbjct: 306 SDS 306

BLAST of Lsi03G016340 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 194.5 bits (493), Expect = 1.0e-49
Identity = 106/230 (46.09%), Postives = 144/230 (62.61%), Query Frame = 0

Query: 1   MPLVSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 60
           M  VSAS  ++N+S T   T LIGA IAD++ GR+WTI    +IY  GM  +TISA +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 61  LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 120
           L  P C  +  C   +G Q    ++AL L +LG GGI+PCV +F ADQFD T        
Sbjct: 123 L-TPTCSGE-TCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 182

Query: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 180
            +FFNW+YF + +  + A +V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   K
Sbjct: 183 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 242

Query: 181 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHT 231
           P GSPL R+ QV+VA+ +  K  +P D +LLY N   +++I    +L HT
Sbjct: 243 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHT 289

BLAST of Lsi03G016340 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 193.0 bits (489), Expect = 3.0e-49
Identity = 102/245 (41.63%), Postives = 150/245 (61.22%), Query Frame = 0

Query: 4   VSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHP 63
           VSA+  +T +  T   T LIGA++AD++ GR+WTI   S IY +GM  +T+SA +P+L P
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 64  PPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNF 123
             C     C  A+  Q    +  L L +LG GGI+PCV +F ADQFD T      R  +F
Sbjct: 142 AECIGDF-CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 201

Query: 124 FNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSG 183
           FNW+YF + +  L + +++V+IQ+N GWG GFGIPT+ M L++ +F  G+PLY   KP G
Sbjct: 202 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 261

Query: 184 SPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAAGH 243
           SP+ R++QVVVA+F+     +P D+ LLY     ++AIA   ++ HTD  +  +  A   
Sbjct: 262 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 321

Query: 244 RTQSR 249
             +S+
Sbjct: 322 EEESK 325

BLAST of Lsi03G016340 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 3.9e-49
Identity = 103/247 (41.70%), Postives = 151/247 (61.13%), Query Frame = 0

Query: 4   VSASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHP 63
           VSA++ +  +  T   T LIGA+IADS+ GR+WTI   S IY +GM  +T+SA LP L P
Sbjct: 93  VSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKP 152

Query: 64  PPCP--TQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTW 123
             C       C+ A+  Q    +  L L +LG GGI+PCV +F ADQFD T      R  
Sbjct: 153 AACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKA 212

Query: 124 NFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKP 183
           +FFNW+YF + + +  + T++V++Q+NVGWG GF IPT+ M +S+ +F +G+PLY   KP
Sbjct: 213 SFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKP 272

Query: 184 SGSPLVRLAQVVVAAFKNRKAVLPHDSNLLYRNHKLDAAIAIQGRLVHTDQFKSTESVAA 243
            GSP+ R+ QV+VAA++  K  LP D + LY   + ++ IA   ++ HTD +K  +  A 
Sbjct: 273 GGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAV 332

Query: 244 GHRTQSR 249
               +S+
Sbjct: 333 ISEYESK 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX203.7e-7660.00Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9M3908.5e-4942.39Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.4e-4846.09Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460324.2e-4841.63Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z205.5e-4841.70Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
Q964001.0e-12193.33Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2[more]
A0A5A7UI293.0e-12192.50Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S4DUU13.0e-12192.50protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 S... [more]
A0A6J1I1C56.4e-11690.00protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE... [more]
A0A6J1FLW34.1e-11589.58protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 ... [more]
Match NameE-valueIdentityDescription
NP_001267552.12.1e-12193.33probable nitrite transporter At1g68570-like [Cucumis sativus] >KGN59416.1 hypoth... [more]
XP_016899751.16.1e-12192.50PREDICTED: protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo][more]
KAA0053906.16.1e-12192.50protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo var. makuwa] >TYK25499.1 protein... [more]
XP_038903972.11.3e-11891.25protein NRT1/ PTR FAMILY 3.1-like isoform X2 [Benincasa hispida][more]
XP_038903971.11.3e-11891.25protein NRT1/ PTR FAMILY 3.1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G68570.12.6e-7760.00Major facilitator superfamily protein [more]
AT3G54140.16.0e-5042.39peptide transporter 1 [more]
AT5G01180.11.0e-4946.09peptide transporter 5 [more]
AT2G02040.13.0e-4941.63peptide transporter 2 [more]
AT1G62200.13.9e-4941.70Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..243
e-value: 4.1E-74
score: 251.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 5..202
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 35..238
e-value: 1.8E-47
score: 162.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..234
NoneNo IPR availablePANTHERPTHR11654:SF326NITRATE TRANSPORTER 1coord: 1..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G016340.1Lsi03G016340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity