Lsi03G016200 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G016200
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Werner Syndrome-like exonuclease
Location	chr03: 27308140 .. 27308769 (+)
RNA-Seq Expression	Lsi03G016200
Synteny	Lsi03G016200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCACCATCGTTGACCATGAAATTCCCTCCGATTCCCACAATTTCTTCGACGTAACTTTCGACTCCGACGAGCCGATTCTCACTCTGCTCACCACTTCACCATCCATGGTAGACAATTGGATTTCGGAAACCCTCGCCATTCAAACTCCACCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAACCCCGTCGCCACCTTGCAACTCTGCATCGGCCGCCGATGCTTGATTTTGCAACTGATCCACACACCTGAGATCCCTAAATCTCTGTTCGAGTTTCTGGAAAACGAATCCTTCACATTTGTAGGAGTGGGAATCGACGAGGATGCTGAAAAGCTCAACTGTGACTACGGATTGAAAGTGGGGAAGAGAGTAGATCTGAGGAATTTGGCTGAGAGTGTAACGGGAAGAGGAGATTTGAAGAATGCGGGATTGAAGAAACTGGGAAAAGAGGTGTTGGGGAAAGAGATTCAGAAGCCGAAGCGTGTGACGATGAGTAGATGGGATCAAGAATGGCTGACTCTTAATCAGGTTAAGTATGCTTGTATTGATGCGTTCTTTTCGTTTGAGATTGGAAGGCATTTGCAATCTTCATCTAATTGA

mRNA sequence

ATGGCGATCACCATCGTTGACCATGAAATTCCCTCCGATTCCCACAATTTCTTCGACGTAACTTTCGACTCCGACGAGCCGATTCTCACTCTGCTCACCACTTCACCATCCATGGTAGACAATTGGATTTCGGAAACCCTCGCCATTCAAACTCCACCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAACCCCGTCGCCACCTTGCAACTCTGCATCGGCCGCCGATGCTTGATTTTGCAACTGATCCACACACCTGAGATCCCTAAATCTCTGTTCGAGTTTCTGGAAAACGAATCCTTCACATTTGTAGGAGTGGGAATCGACGAGGATGCTGAAAAGCTCAACTGTGACTACGGATTGAAAGTGGGGAAGAGAGTAGATCTGAGGAATTTGGCTGAGAGTGTAACGGGAAGAGGAGATTTGAAGAATGCGGGATTGAAGAAACTGGGAAAAGAGGTGTTGGGGAAAGAGATTCAGAAGCCGAAGCGTGTGACGATGAGTAGATGGGATCAAGAATGGCTGACTCTTAATCAGGTTAAGTATGCTTGTATTGATGCGTTCTTTTCGTTTGAGATTGGAAGGCATTTGCAATCTTCATCTAATTGA

Coding sequence (CDS)

ATGGCGATCACCATCGTTGACCATGAAATTCCCTCCGATTCCCACAATTTCTTCGACGTAACTTTCGACTCCGACGAGCCGATTCTCACTCTGCTCACCACTTCACCATCCATGGTAGACAATTGGATTTCGGAAACCCTCGCCATTCAAACTCCACCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAACCCCGTCGCCACCTTGCAACTCTGCATCGGCCGCCGATGCTTGATTTTGCAACTGATCCACACACCTGAGATCCCTAAATCTCTGTTCGAGTTTCTGGAAAACGAATCCTTCACATTTGTAGGAGTGGGAATCGACGAGGATGCTGAAAAGCTCAACTGTGACTACGGATTGAAAGTGGGGAAGAGAGTAGATCTGAGGAATTTGGCTGAGAGTGTAACGGGAAGAGGAGATTTGAAGAATGCGGGATTGAAGAAACTGGGAAAAGAGGTGTTGGGGAAAGAGATTCAGAAGCCGAAGCGTGTGACGATGAGTAGATGGGATCAAGAATGGCTGACTCTTAATCAGGTTAAGTATGCTTGTATTGATGCGTTCTTTTCGTTTGAGATTGGAAGGCATTTGCAATCTTCATCTAATTGA

Protein sequence

MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDIEWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQEWLTLNQVKYACIDAFFSFEIGRHLQSSSN

Homology

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BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.0e-19
Identity = 59/153 (38.56%), Postives = 91/153 (59.48%), Query Frame = 0


Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCI-GRRCLILQLIHTPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H+  IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRV 175
           +GID D+ KL  DYG+ +    DL +LA    G GD K  GL  L + ++ KE+ KP R+
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGRHLQ 206
            +  W+   L+  Q++YA  DA+ S+ + + L+
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 8.6e-15
Identity = 58/151 (38.41%), Postives = 77/151 (50.99%), Query Frame = 0


Query: 55  IVGLDIEWRPNNRSYDNPVATLQLCIGR-RCLILQLIHTPEIPKSLFEFLENESFTFVGV 114
           ++G D EW     S   PVA LQL   R  C + +L H  +IP+ L E LE++S   VGV
Sbjct: 79  VLGFDCEWITVGGS-RRPVALLQLSSHRGLCALFRLCHMKQIPQDLRELLEDDSVIKVGV 138

Query: 115 GIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVT 174
              EDA KL+ DYG+ V   +DLR L   + G    K  GL KL K  L   + K  R+ 
Sbjct: 139 APQEDAMKLSHDYGVGVASTLDLRFLC-VMAGH---KPEGLGKLSKTHLNYTLDKHWRLA 198

Query: 175 MSRWDQEWLTLNQVKYACIDAFFSFEIGRHL 205
            S W+ + L   Q+ YA  DA  +  I + L
Sbjct: 199 CSNWEAKTLEPKQLDYAANDALMAVAIYQKL 224

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BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 52/153 (33.99%), Postives = 79/153 (51.63%), Query Frame = 0


Query: 55  IVGLDIEWRP-NNRSYDNPVATLQLCIGR-RCLILQLIHTPEIPKSLFEFLENESFTFVG 114
           +VG D+EW P  NR     VA +QLC+   +C +  +      P+ L   LEN++    G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 115 VGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRV 174
           VGI+ D  KL  D+ +K+   V+L ++A         +   L  L K +LGK++ K K +
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKL--KCTETWSLNSLVKHLLGKQLLKDKSI 197

Query: 175 TMSRWDQEWLTLNQVKYACIDAFFSFEIGRHLQ 206
             S W +  LT +Q  YA  DA+  F I R+L+
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLE 228

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BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 53/149 (35.57%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 55  IVGLDIEW----RPNNRSYDNPVATLQLCIGR-RCLILQLIHTPEIPKSLFEFLENESFT 114
           +VG D+EW    +P  RS    VA +QLC+   +C +  +      P+ L   LEN+S  
Sbjct: 72  VVGFDMEWPPIYKPGKRS---RVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIK 131

Query: 115 FVGVGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNA---GLKKLGKEVLGKEI 174
             GVGI+ D  KL  D+ +K+   V+L ++A        LK A    L  L K VLGK++
Sbjct: 132 KAGVGIEGDQWKLLRDFDVKLESFVELTDVANE-----KLKCAETWSLNGLVKHVLGKQL 191

Query: 175 QKPKRVTMSRWDQEWLTLNQVKYACIDAF 196
            K K +  S W    LT +Q  YA  DA+
Sbjct: 192 LKDKSIRCSNWSNFPLTEDQKLYAATDAY 212

[Close]

BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot
Match: Q9NVH0 (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 53/155 (34.19%), Postives = 76/155 (49.03%), Query Frame = 0


Query: 55  IVGLDIEWRPNNRSYDNPVATLQLCI-GRRCLILQL----IHTPEIPKSLFEFLENESFT 114
           ++G+D EW  N     +P++ LQ+      C++++L         +P++L + L + +  
Sbjct: 104 VLGIDCEW-VNLEGKASPLSLLQMASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTIL 163

Query: 115 FVGVGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKP 174
            VGVG  EDA KL  DYGL V   +DLR LA             LK L + VL   + K 
Sbjct: 164 KVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPLDKS 223

Query: 175 KRVTMSRWDQEWLTLNQVKYACIDAFFSFEIGRHL 205
             +  S WD E LT +QV YA  DA  S  +  HL
Sbjct: 224 LLLRCSNWDAETLTEDQVIYAARDAQISVALFLHL 257

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BLAST of Lsi03G016200 vs. ExPASy TrEMBL
Match: A0A0A0LBN3 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.5e-107
Identity = 190/208 (91.35%), Postives = 203/208 (97.60%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MAITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD WIS+TLAIQTPPL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSS 209
           WLTLNQVKYACIDAFFSFEIG+ LQS+S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]

BLAST of Lsi03G016200 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.4e-107
Identity = 189/209 (90.43%), Postives = 203/209 (97.13%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           M+ITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD+WIS+TL IQTPPL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLTLNQVKYACIDAFFSFEIGR LQS S+
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of Lsi03G016200 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.4e-107
Identity = 189/209 (90.43%), Postives = 203/209 (97.13%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           M+ITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD+WIS+TL IQTPPL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLTLNQVKYACIDAFFSFEIGR LQS S+
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of Lsi03G016200 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.4e-107
Identity = 189/209 (90.43%), Postives = 203/209 (97.13%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           M+ITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD+WIS+TL IQTPPL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLTLNQVKYACIDAFFSFEIGR LQS S+
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of Lsi03G016200 vs. ExPASy TrEMBL
Match: A0A6J1F975 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 167/209 (79.90%), Postives = 186/209 (89.00%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MA+TIVD E PS SHN+FDVTF+SDEPILTLLT SP MVD+WISETL  QT PLIVGLDI
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QLI+ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKV K VDLRNLAESVTGR +LKNAGLK L KE+LGKEI+KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLT NQ+KYAC DAF S+EIGRHLQ+SS+
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 209

[Close]

BLAST of Lsi03G016200 vs. NCBI nr
Match: XP_011652174.1 (Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical protein Csa_001395 [Cucumis sativus])

HSP 1 Score: 398.7 bits (1023), Expect = 3.2e-107
Identity = 190/208 (91.35%), Postives = 203/208 (97.60%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MAITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD WIS+TLAIQTPPL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSS 209
           WLTLNQVKYACIDAFFSFEIG+ LQS+S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]

BLAST of Lsi03G016200 vs. NCBI nr
Match: XP_008443244.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exonuclease [Cucumis melo subsp. melo] >KAA0053896.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK25510.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 397.5 bits (1020), Expect = 7.1e-107
Identity = 189/209 (90.43%), Postives = 203/209 (97.13%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           M+ITIVDHE+PSDSHN+FD+TFD+DEPILTLLTTSPSMVD+WIS+TL IQTPPL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQL+H PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGRG+LKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLTLNQVKYACIDAFFSFEIGR LQS S+
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of Lsi03G016200 vs. NCBI nr
Match: XP_038903327.1 (Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida])

HSP 1 Score: 358.2 bits (918), Expect = 4.7e-95
Identity = 171/187 (91.44%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MAITIVDH++PSDSHN+FD+T DSDEPILTLLT SPSMV+NWISETLA+QTPPL+VGLDI
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLC+GRRCLILQLIHTPEIPKSLFEFLENES TFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKRVDLRNLAESVTGR DLKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQV 188
           WLTLNQ+
Sbjct: 181 WLTLNQM 187

[Close]

BLAST of Lsi03G016200 vs. NCBI nr
Match: XP_038903326.1 (Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 6.2e-95
Identity = 171/186 (91.94%), Postives = 180/186 (96.77%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MAITIVDH++PSDSHN+FD+T DSDEPILTLLT SPSMV+NWISETLA+QTPPL+VGLDI
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLC+GRRCLILQLIHTPEIPKSLFEFLENES TFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKVGKRVDLRNLAESVTGR DLKNAGLK+LGKEVLGKEI KPK VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQ 187
           WLTLNQ
Sbjct: 181 WLTLNQ 186

[Close]

BLAST of Lsi03G016200 vs. NCBI nr
Match: KAG6580879.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017635.1 Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348.6 bits (893), Expect = 3.8e-92
Identity = 168/209 (80.38%), Postives = 187/209 (89.47%), Query Frame = 0


Query: 1   MAITIVDHEIPSDSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAIQTPPLIVGLDI 60
           MA+TIVD E PS SHN+FDVTF+SDEPILTLLT SP MVD+WISETL  QTPPLIVGLDI
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTPPLIVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QLI+ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRVTMSRWDQE 180
           KLNCDYGLKV K VDLRNLAESVTGR +LKNAGLK L KE+LGKEI+KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGRHLQSSSN 210
           WLT NQ+KYAC DAF S+EIGRHLQ+SS+
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 209

[Close]

BLAST of Lsi03G016200 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 4.0e-23
Identity = 69/212 (32.55%), Postives = 113/212 (53.30%), Query Frame = 0


Query: 13  DSHNFFDVTFDSDEPILTLLTTSPSMVDNWISETL---AIQTPPLIVGLDIEW------- 72
           ++H  + V F  DE I+T +T   S++  WI   L      + PL+VG+ ++W       
Sbjct: 13  NTHLEYSVDFFGDEFIVT-VTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYS 72

Query: 73  --RPNN--------RSY-DNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFV 132
             RPNN        R Y DNP   LQLC+G RCLI+QL +  ++P +L  FL +   TFV
Sbjct: 73  DPRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFV 132

Query: 133 GVGIDEDAEKL-NCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKE-IQKP 192
           GV   +DA KL  C + L++G+ +D+R       GR  ++ +  +++ +E +G + +   
Sbjct: 133 GVWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWGR-SMRRSSFEEIVEECMGYQGVMLD 192

Query: 193 KRVTMSRWDQEWLTLNQVKYACIDAFFSFEIG 202
             ++MS W    L L+Q+  A +DA+   ++G
Sbjct: 193 PEISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of Lsi03G016200 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 9.7e-22
Identity = 62/182 (34.07%), Postives = 99/182 (54.40%), Query Frame = 0


Query: 26  EPILTLLTTSPSMVDNWISE----TLAIQTPPLIVGLDIEWRPNNRSYDNPVATLQLCIG 85
           E ++  +T + S +  WI      +    + PL+VGLD++W P     D P   LQLC+G
Sbjct: 24  ERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS--DPPPDILQLCVG 83

Query: 86  RRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGLKVGKRVDLRNLA 145
            RCLI+QL H   IP+ L  FLE+E+ TFVGV   +D  KL    + L++ + +D+R+  
Sbjct: 84  NRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRHYL 143

Query: 146 ESVTGRGDLKNAGLKKLGKEVLG-KEIQKPKRVTMSRWDQEWLTLNQVKYACIDAFFSFE 202
            +      L N+  +K+ +E LG K ++K K + MS W    L+ +Q+  A  D +   +
Sbjct: 144 PT-----RLLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVYVCCK 198

[Close]

BLAST of Lsi03G016200 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.2e-21
Identity = 59/202 (29.21%), Postives = 99/202 (49.01%), Query Frame = 0


Query: 26  EPILTLLTTSPSMVDNWISETLA---IQTPPLIVGLDIEWRPNN---------------- 85
           E  + ++T  PS++  WI + L+     + PL+VG+ ++W P++                
Sbjct: 24  EEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRYRSDSPP 83

Query: 86  -----------RSYDNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGI 145
                      R +D P  TLQLC+G RC+I+QL H   +P+ L  FL +  +TFVG+  
Sbjct: 84  VRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDYTFVGIWN 143

Query: 146 DEDAEKL-NCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKE-IQKPKRVT 196
            +DA KL    + L++   +DLR      +GR  +K    +K+ +E LG   ++  ++V+
Sbjct: 144 SQDAGKLKRSKHELEIAVLLDLRKFVSDSSGR-TMKRCSFEKIVEENLGHSGVRLDRKVS 203

[Close]

BLAST of Lsi03G016200 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-20
Identity = 63/193 (32.64%), Postives = 103/193 (53.37%), Query Frame = 0


Query: 14  SHNFFDVTFDSDEPILTLLTTSPSMVDNWISETLAI----QTPPLIVGLDIEWRPNNRSY 73
           +H    V F  D+ I+T +T + S++  WI    +        PL+VG+ ++WRP++   
Sbjct: 13  THQKHFVDFFGDDLIVT-VTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  DNPVATLQLCIGRRCLILQLIHTPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGL 133
           D P  TLQLC+G RCLI+QL +   +PK L  FL +   TFVGV   +D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGK-EIQKPKRVTMSRWDQEWLTLNQV 193
           ++GK +D+R       G   +     +++ KE LG+  ++    + MS W    L   QV
Sbjct: 133 EIGKLLDIRMFVRDSRG-ARMCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACIDAFFSFEI 201
             A I+++  F++
Sbjct: 193 LQASIESYVCFKL 203

[Close]

BLAST of Lsi03G016200 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-20
Identity = 59/152 (38.82%), Postives = 90/152 (59.21%), Query Frame = 0


Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCI-GRRCLILQLIHTPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H+  IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRVDLRNLAESVTGRGDLKNAGLKKLGKEVLGKEIQKPKRV 175
           +GID D+ KL  DYG+ +    DL +LA    G GD K  GL  L + ++ KE+ KP R+
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGRHL 205
            +  W+   L+  Q++YA  DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

The following BLAST results are available for this feature:

BLAST of Lsi03G016200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	2.0e-19	38.56	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q9VGN7	8.6e-15	38.41	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
Q14191	1.1e-14	33.99	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	1.1e-12	35.57	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9NVH0	1.8e-12	34.19	Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE...	[more]

BLAST of Lsi03G016200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBN3	1.5e-107	91.35	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7UI20	3.4e-107	90.43	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	3.4e-107	90.43	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	3.4e-107	90.43	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]
A0A6J1F975	1.5e-91	79.90	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 P...	[more]

BLAST of Lsi03G016200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652174.1	3.2e-107	91.35	Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical prot...	[more]
XP_008443244.1	7.1e-107	90.43	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exo...	[more]
XP_038903327.1	4.7e-95	91.44	Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida]	[more]
XP_038903326.1	6.2e-95	91.94	Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida]	[more]
KAG6580879.1	3.8e-92	80.38	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Lsi03G016200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12410.1	4.0e-23	32.55	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	9.7e-22	34.07	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12430.1	8.2e-21	29.21	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12470.1	1.4e-20	32.64	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.1	1.4e-20	38.82	Werner syndrome-like exonuclease	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 36..209 e-value: 2.8E-10 score: 50.2
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 36..206 e-value: 8.8E-18 score: 64.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 18..206 e-value: 5.4E-58 score: 197.8
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 9..208
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 9..208
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..204 e-value: 2.73352E-65 score: 197.031
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 45..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G016200.1	Lsi03G016200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding