Lsi03G015000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G015000
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF hand family protein
Location	chr03: 26013983 .. 26014502 (-)
RNA-Seq Expression	Lsi03G015000
Synteny	Lsi03G015000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAACCGATTAGCCGACGTCAACGTGCAGTTCGTCCCGACGCCGCTCGTGTTCATCGATCGGATATCGATTTTACCAATTTTGTTCACCTTTGCAGACAAATTACATGACCTTATCATCAAATCTCTACCTTTTTCACTTTTTCAATCACCATCCAAAGAGAAGACTTCGTCACCAACTAATTCTACTTCAGAATCTGATGATCAGTTCATCACTCAACAAAAGAATTGCTTAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCAGCCAAGAAGGAGATGATGATGATGATGATAAAGGAATTGGAGAATGTTTTGGAGGTGATGAATTAACAGCTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTCGATAAAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACAGAGTTCTTGGCTTTCTTGGTTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGA

mRNA sequence

ATGCAAAACCGATTAGCCGACGTCAACGTGCAGTTCGTCCCGACGCCGCTCGTGTTCATCGATCGGATATCGATTTTACCAATTTTGTTCACCTTTGCAGACAAATTACATGACCTTATCATCAAATCTCTACCTTTTTCACTTTTTCAATCACCATCCAAAGAGAAGACTTCGTCACCAACTAATTCTACTTCAGAATCTGATGATCAGTTCATCACTCAACAAAAGAATTGCTTAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCAGCCAAGAAGGAGATGATGATGATGATGATAAAGGAATTGGAGAATGTTTTGGAGAGATGGGTTCATTGATGCACAGGAATTGCACAGAGTTCTTGGCTTTCTTGGTTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGA

Coding sequence (CDS)

ATGCAAAACCGATTAGCCGACGTCAACGTGCAGTTCGTCCCGACGCCGCTCGTGTTCATCGATCGGATATCGATTTTACCAATTTTGTTCACCTTTGCAGACAAATTACATGACCTTATCATCAAATCTCTACCTTTTTCACTTTTTCAATCACCATCCAAAGAGAAGACTTCGTCACCAACTAATTCTACTTCAGAATCTGATGATCAGTTCATCACTCAACAAAAGAATTGCTTAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCAGCCAAGAAGGAGATGATGATGATGATGATAAAGGAATTGGAGAATGTTTTGGAGAGATGGGTTCATTGATGCACAGGAATTGCACAGAGTTCTTGGCTTTCTTGGTTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGA

Protein sequence

MQNRLADVNVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSSPTNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIGECFGEMGSLMHRNCTEFLAFLVLIKAYSSMTAKE

Homology

[Close]

BLAST of Lsi03G015000 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 9.8e-21
Identity = 67/114 (58.77%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 1   MQNRLADV-NVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSS 60
           M+ RL D+ +VQ  P+P VF+DRISILPILF FAD++ +L ++SLP+ + Q  SKEK  S
Sbjct: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQEL-VRSLPWPILQPHSKEKALS 71

Query: 61  PTNSTSESDDQ-FITQQKNC-LMSREEVRFVMEKLELFCSQEGDDDDDDKGIGE 112
             N +S+S DQ  IT Q+NC  MSREEVRFVMEKLELF  +E D    DKGIGE
Sbjct: 72  QCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGE 124

[Close]

BLAST of Lsi03G015000 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.2e-20
Identity = 66/105 (62.86%), Postives = 82/105 (78.10%), Query Frame = 0


Query: 9   NVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPF-SLFQSP-SKEKTSSPTNSTSE 68
           +VQ  P+PLVF+D IS LPILF FA ++H+L I+SLP+ S+ QS  SKEK  S +N +S+
Sbjct: 11  SVQLDPSPLVFVDWISNLPILFNFAGRIHEL-IRSLPYWSILQSQYSKEKALSQSNCSSQ 70

Query: 69  SDDQ-FITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIG 111
           SDD+  IT Q+NC MSREEVRFVMEKLELF  +E DDDDDD+ IG
Sbjct: 71  SDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIG 114

[Close]

BLAST of Lsi03G015000 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-13
Identity = 58/121 (47.93%), Postives = 77/121 (63.64%), Query Frame = 0


Query: 1   MQNRLADVNVQFVPT-------PLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPS 60
           M N    +N+Q +P+       PLVFIDR SI      FAD +HDL+  SL FS FQSP 
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLV--SLAFSFFQSPP 60

Query: 61  KEKTSSPTNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIGECF 115
           KEK ++P +  S+S+      +++ LMSREEV+ VMEKLEL+C++EGDD+     I ECF
Sbjct: 61  KEK-AAPQSRASQSEG-----KEDWLMSREEVKSVMEKLELYCNEEGDDE-----IDECF 102

[Close]

BLAST of Lsi03G015000 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.4e-13
Identity = 53/99 (53.54%), Postives = 69/99 (69.70%), Query Frame = 0


Query: 1   MQNRLADVNVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSSP 60
           M ++   VN++  PT L+ +DRISI  ILF+F D++HDLI  S P+SLF SP KEK  SP
Sbjct: 1   MPSQFPIVNLKLAPT-LLLVDRISISSILFSFVDRIHDLI--SQPWSLFHSPPKEKV-SP 60

Query: 61  TNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQE 100
             ++ ES+    TQ KN  +S+EEV+FVMEKLE FCS E
Sbjct: 61  QGNSLESE---ATQHKNSSISKEEVKFVMEKLEFFCSDE 92

[Close]

BLAST of Lsi03G015000 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 3.7e-12
Identity = 52/99 (52.53%), Postives = 69/99 (69.70%), Query Frame = 0


Query: 1   MQNRLADVNVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSSP 60
           M ++   VN++   T L+ +DRISI  ILF+F D++HDLI  S P+SLF SP KEK +SP
Sbjct: 1   MPSQFPVVNLKLALT-LLLVDRISISSILFSFVDRIHDLI--SQPWSLFHSPPKEK-ASP 60

Query: 61  TNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQE 100
             ++ ES+    TQ KN  +S+EEV+FVMEKLE FCS E
Sbjct: 61  QGNSLESE---ATQHKNSSISKEEVKFVMEKLEFFCSDE 92

[Close]

BLAST of Lsi03G015000 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 160.2 bits (404), Expect = 1.3e-35
Identity = 86/125 (68.80%), Postives = 101/125 (80.80%), Query Frame = 0


Query: 1   MQNRLADVNVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSSP 60
           MQNRL DVNVQ +PTPLVF+DR SILPILFTFAD++HDLI  S+P S FQSPSKEK S  
Sbjct: 1   MQNRLPDVNVQLIPTPLVFVDRTSILPILFTFADRIHDLI--SVPLSFFQSPSKEKASPQ 60

Query: 61  TNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIGECFG--EMGS 120
           +N +S+SDDQ ITQQKNCLMSREEVRFVM+KLELFCS+    +D D+ IGECFG  E+ +
Sbjct: 61  SNCSSQSDDQVITQQKNCLMSREEVRFVMDKLELFCSK----NDGDEEIGECFGGEELTA 119

Query: 121 LMHRN 124
           +   N
Sbjct: 121 MFEEN 119

[Close]

BLAST of Lsi03G015000 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 67/114 (58.77%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 1   MQNRLADV-NVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSS 60
           M+ RL D+ +VQ  P+P VF+DRISILPILF FAD++ +L ++SLP+ + Q  SKEK  S
Sbjct: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQEL-VRSLPWPILQPHSKEKALS 71

Query: 61  PTNSTSESDDQ-FITQQKNC-LMSREEVRFVMEKLELFCSQEGDDDDDDKGIGE 112
             N +S+S DQ  IT Q+NC  MSREEVRFVMEKLELF  +E D    DKGIGE
Sbjct: 72  QCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGE 124

[Close]

BLAST of Lsi03G015000 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 67/114 (58.77%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 1   MQNRLADV-NVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPSKEKTSS 60
           M+ RL D+ +VQ  P+P VF+DRISILPILF FAD++ +L ++SLP+ + Q  SKEK  S
Sbjct: 1   METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQEL-VRSLPWPILQPHSKEKALS 60

Query: 61  PTNSTSESDDQ-FITQQKNC-LMSREEVRFVMEKLELFCSQEGDDDDDDKGIGE 112
             N +S+S DQ  IT Q+NC  MSREEVRFVMEKLELF  +E D    DKGIGE
Sbjct: 61  QCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGE 113

[Close]

BLAST of Lsi03G015000 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 108.6 bits (270), Expect = 4.5e-20
Identity = 66/105 (62.86%), Postives = 82/105 (78.10%), Query Frame = 0


Query: 9   NVQFVPTPLVFIDRISILPILFTFADKLHDLIIKSLPF-SLFQSP-SKEKTSSPTNSTSE 68
           +VQ  P+PLVF+D IS LPILF FA ++H+L I+SLP+ S+ QS  SKEK  S +N +S+
Sbjct: 11  SVQLDPSPLVFVDWISNLPILFNFAGRIHEL-IRSLPYWSILQSQYSKEKALSQSNCSSQ 70

Query: 69  SDDQ-FITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIG 111
           SDD+  IT Q+NC MSREEVRFVMEKLELF  +E DDDDDD+ IG
Sbjct: 71  SDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIG 114

[Close]

BLAST of Lsi03G015000 vs. NCBI nr
Match: XP_022983385.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 85.9 bits (211), Expect = 3.1e-13
Identity = 58/121 (47.93%), Postives = 77/121 (63.64%), Query Frame = 0


Query: 1   MQNRLADVNVQFVPT-------PLVFIDRISILPILFTFADKLHDLIIKSLPFSLFQSPS 60
           M N    +N+Q +P+       PLVFIDR SI      FAD +HDL+  SL FS FQSP 
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLV--SLAFSFFQSPP 60

Query: 61  KEKTSSPTNSTSESDDQFITQQKNCLMSREEVRFVMEKLELFCSQEGDDDDDDKGIGECF 115
           KEK ++P +  S+S+      +++ LMSREEV+ VMEKLEL+C++EGDD+     I ECF
Sbjct: 61  KEK-AAPQSRASQSEG-----KEDWLMSREEVKSVMEKLELYCNEEGDDE-----IDECF 102

The following BLAST results are available for this feature:

BLAST of Lsi03G015000 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi03G015000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ5	9.8e-21	58.77	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A1S3B8I1	2.2e-20	62.86	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A6J1J5Q6	1.5e-13	47.93	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1KQS0	3.4e-13	53.54	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1EIK8	3.7e-12	52.53	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Lsi03G015000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904514.1	1.3e-35	68.80	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
KGN59556.1	2.0e-20	58.77	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_004147503.1	2.0e-20	58.77	probable calcium-binding protein CML45 [Cucumis sativus]	[more]
XP_008443616.1	4.5e-20	62.86	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_022983385.1	3.1e-13	47.93	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]

BLAST of Lsi03G015000 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G015000.1	Lsi03G015000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding