Lsi03G014970 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G014970 vs. ExPASy Swiss-Prot
Match: Q43847 (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=SS2 PE=1 SV=3)

HSP 1 Score: 911.8 bits (2355), Expect = 6.1e-264
Identity = 489/815 (60.00%), Postives = 584/815 (71.66%), Query Frame = 0

Query: 67  ATSDSSGQ--RNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVDSKEE 126
           AT ++SG+    D+ ++A+Q TIEKSKKVLAMQQ LL+QIAER+K+VSSIKS+  ++K  
Sbjct: 46  ATGENSGEAASADESNDALQVTIEKSKKVLAMQQDLLQQIAERRKVVSSIKSSLANAKGT 105

Query: 127 QDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRSSDKN 186
            D      G     D P  + +  V V + ++  +++    V +    A+S     S + 
Sbjct: 106 YDGG---SGSLSDVDIPDVDKDYNVTVPSTAATPITD----VDKNTPPAISQDFVESKRE 165

Query: 187 DEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPKDQLK 246
            ++ + D +     A P   L  SS T  S I          SS   S   L  P +  K
Sbjct: 166 IKRDLADER-----APP---LSRSSITASSQI----------SSTVSSKRTLNVPPETPK 225

Query: 247 GSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTLKVDS 306
            SS++   D+  +S+  L     K    ++P+ L  +SS S V E ++E     + + + 
Sbjct: 226 -SSQETLLDV--NSRKSLVDVPGKKIQSYMPS-LRKESSASHV-EQRNENLEGSSAEANE 285

Query: 307 DAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVI 366
           +  +  ++D KP PLAG NVMN+I+VA+ECAPWSKTGGLGDVAG+LPKALARRGHRVMV+
Sbjct: 286 ETEDPVNIDEKPPPLAGTNVMNIILVASECAPWSKTGGLGDVAGALPKALARRGHRVMVV 345

Query: 367 VPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIYGGDH 426
            PRY NY   QD+G+RK YKVDGQD EV+YFQ ++DGVDFVFID++MF H   NIYGG+ 
Sbjct: 346 APRYDNYPEPQDSGVRKIYKVDGQDVEVTYFQAFIDGVDFVFIDSHMFRHIGNNIYGGNR 405

Query: 427 LDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDFGSVE 486
           +DILKRMVLFCKAA                                            +E
Sbjct: 406 VDILKRMVLFCKAA--------------------------------------------IE 465

Query: 487 VPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVC 546
           VPW+VPCGGVCYGDGNLVF+ANDWHTA+LPVYLKAYYRDNGIM +TRS+LVIHNIAHQ  
Sbjct: 466 VPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGIMNYTRSVLVIHNIAHQ-- 525

Query: 547 YSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGLKTAD 606
                            GRGP+ DF +VDLP HY+D FKLYDPVGGEHFNIFAAGLKTAD
Sbjct: 526 -----------------GRGPLEDFSYVDLPPHYMDPFKLYDPVGGEHFNIFAAGLKTAD 585

Query: 607 RVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTN 666
           RVVTVSHGY+WELKTS+GGWGLH IINE DWK+ GIVNGID+KEW+P+ D+HL SDGY N
Sbjct: 586 RVVTVSHGYSWELKTSQGGWGLHQIINENDWKLQGIVNGIDTKEWNPELDVHLQSDGYMN 645

Query: 667 YSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQ 726
           YSL+TL TGKPQCKAALQKELGLP+R+DVPLIGFIGRLD QKGVD+IAEA+PWM+GQDVQ
Sbjct: 646 YSLDTLQTGKPQCKAALQKELGLPVRDDVPLIGFIGRLDPQKGVDLIAEAVPWMMGQDVQ 705

Query: 727 LVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQ 786
           LVMLGTGR+DLEQMLR FE QH DKIRGWVGFSVK +HRITAG DILLMPSRFEPCGLNQ
Sbjct: 706 LVMLGTGRRDLEQMLRQFECQHNDKIRGWVGFSVKTSHRITAGADILLMPSRFEPCGLNQ 765

Query: 787 LYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTYRQYK 846
           LYAM YGTIPVVH+VGGLRDTV PFDP+NESGLGWTF  AEA++LI+ALGNCLLTYR+YK
Sbjct: 766 LYAMKYGTIPVVHAVGGLRDTVQPFDPFNESGLGWTFSRAEASQLIHALGNCLLTYREYK 767

Query: 847 KSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           KSWEG+Q R MTQDLSWD+AA+ YEEVL+AAKYQW
Sbjct: 826 KSWEGIQTRCMTQDLSWDNAAQNYEEVLIAAKYQW 767

BLAST of Lsi03G014970 vs. ExPASy Swiss-Prot
Match: Q9MAC8 (Starch synthase 2, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SS2 PE=1 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 4.4e-262
Identity = 485/810 (59.88%), Postives = 571/810 (70.49%), Query Frame = 0

Query: 70  DSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVDSKEEQDSNE 129
           ++SG  +D+P++A+QATI+KSKKVLAMQ+ LL QIAER+KLVSSIK ++ D  + + S++
Sbjct: 61  EASGSDDDEPEDALQATIDKSKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSK 120

Query: 130 QVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRSSDKNDEKPV 189
           Q        +  +   E      NGS    + G  ++ ++ +    S S  S KN     
Sbjct: 121 QESASSVNANTDATKKEIMDGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKN----- 180

Query: 190 VDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPKDQLKGSSKK 249
               +K  SA        +S  K SD+  + K      S   +S V P  K     +S +
Sbjct: 181 ----RKQSSASVISSSPVTSPQKPSDVATNGKPW----SSVVASSVDPPYKPSSVMTSPE 240

Query: 250 KSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTLKVDSDAVET 309
           K+SD +       K  +    SD +P++L+     ST+K  K  + +       S+  E 
Sbjct: 241 KTSDPVTSPGKPSKSRAGAFWSDPLPSYLTKAPQTSTMKTEKYVEKTPDV--ASSETNEP 300

Query: 310 ASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYA 369
              + KP PLAG NVMNVI+VAAECAP+SKTGGLGDVAG+LPK+LARRGHRVMV+VPRYA
Sbjct: 301 GKDEEKPPPLAGANVMNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRYA 360

Query: 370 NYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIYGGDHLDILK 429
            YA  +D G+RKRYKV GQD EV YF  ++DGVDFVFID+  F H   NIYGG+ LDILK
Sbjct: 361 EYAEAKDLGVRKRYKVAGQDMEVMYFHAFIDGVDFVFIDSPEFRHLSNNIYGGNRLDILK 420

Query: 430 RMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYV 489
           RMVLFCKAA                                            VEVPWYV
Sbjct: 421 RMVLFCKAA--------------------------------------------VEVPWYV 480

Query: 490 PCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLS 549
           PCGGVCYGDGNL F+ANDWHTA+LPVYLKAYYRD+GIM +TRS+LVIHNIAHQ       
Sbjct: 481 PCGGVCYGDGNLAFIANDWHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQ------- 540

Query: 550 LILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGLKTADRVVTV 609
                       GRGPV+DF +VDLP HY+D FKLYDPVGGEHFNIFAAGLK ADRV+TV
Sbjct: 541 ------------GRGPVDDFSYVDLPSHYLDSFKLYDPVGGEHFNIFAAGLKAADRVLTV 600

Query: 610 SHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLET 669
           SHGY+WE+KT EGGWGLH IINE DWK  GIVNGID++EW+P++D +L SD YTNYSLE 
Sbjct: 601 SHGYSWEVKTLEGGWGLHNIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDYTNYSLEN 660

Query: 670 LHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLG 729
           LH GKPQCKAALQKELGLP+R DVPLIGFIGRLD QKGVD+IAEA+PWM+ QDVQLVMLG
Sbjct: 661 LHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMMSQDVQLVMLG 720

Query: 730 TGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMN 789
           TGR DLE++LR  E+Q+RDK RGWVGFSVK AHRITAG DILLMPSRFEPCGLNQLYAMN
Sbjct: 721 TGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMN 780

Query: 790 YGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTYRQYKKSWEG 849
           YGTIPVVH+VGGLRDTV  FDPY+E+GLGWTFDSAEA KLI+ALGNCLLTYR+YK+SWEG
Sbjct: 781 YGTIPVVHAVGGLRDTVQQFDPYSETGLGWTFDSAEAGKLIHALGNCLLTYREYKESWEG 792

Query: 850 LQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           LQRRGMTQDLSWD+AAEKYEEVLVAAKY W
Sbjct: 841 LQRRGMTQDLSWDNAAEKYEEVLVAAKYHW 792

BLAST of Lsi03G014970 vs. ExPASy Swiss-Prot
Match: Q43093 (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 2.0e-251
Identity = 471/839 (56.14%), Postives = 564/839 (67.22%), Query Frame = 0

Query: 43  GLGFLGDKMVVRSGKWPKPVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLE 102
           G+G L    V  + K       K+  +D +G  ++  D+ + ATIEKSKKVLA+Q++L++
Sbjct: 39  GVGRLNCGSVRLNHKQHVRAVGKSFGADENGDGSE--DDVVNATIEKSKKVLALQRELIQ 98

Query: 103 QIAERKKLVSSIKSTSVDSKEEQD-SNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSN 162
           QIAERKKLVSSI S S+   E    S E  +        P +   S    +         
Sbjct: 99  QIAERKKLVSSIDSDSIPGLEGNGVSYESSEKSLSRDSNPQKGSSSSGSAVETKRWHCFQ 158

Query: 163 GNVAVREQLDVALSSSSRSSDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLK 222
                +E    A+SS   +   +      +++KK       D +K SSK  +++    +K
Sbjct: 159 QLCRSKETETWAVSSVGINQGFD------EIEKK------NDAVKASSKLHFNE---QIK 218

Query: 223 DQLKESSKTKSSEVLPDPKDQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSK 282
           ++L E          PD KD                                    +SS 
Sbjct: 219 NKLYER---------PDTKD------------------------------------ISSS 278

Query: 283 SSYSTVKEVKDEQPSEPTLK-VDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKT 342
              S++K    E  +EP+ K V ++A    S   KP PLAG NVMN+I+V+AECAPWSKT
Sbjct: 279 IRTSSLKFENFEGANEPSSKEVANEAENFESGGEKPPPLAGTNVMNIILVSAECAPWSKT 338

Query: 343 GGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVD 402
           GGLGDVAGSLPKALARRGHRVM++ P Y NYA   D G+RKRYKV GQD EV+YF TY+D
Sbjct: 339 GGLGDVAGSLPKALARRGHRVMIVAPHYGNYAEAHDIGVRKRYKVAGQDMEVTYFHTYID 398

Query: 403 GVDFVFIDNNMFHHREQNIYGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVL 462
           GVD VFID+ +F + E NIYGG+ LDIL+RMVLFCKAA                      
Sbjct: 399 GVDIVFIDSPIFRNLESNIYGGNRLDILRRMVLFCKAA---------------------- 458

Query: 463 NFNEDRLQKGRVITVDPEDFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAY 522
                                 VEVPW+VPCGG+CYGDGNLVF+ANDWHTA+LPVYLKAY
Sbjct: 459 ----------------------VEVPWHVPCGGICYGDGNLVFIANDWHTALLPVYLKAY 518

Query: 523 YRDNGIMTFTRSILVIHNIAHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYID 582
           YRD+G+M +TRS+LVIHNIAHQ                   GRGPV DF  VDL  +Y+D
Sbjct: 519 YRDHGLMNYTRSVLVIHNIAHQ-------------------GRGPVEDFNTVDLSGNYLD 578

Query: 583 IFKLYDPVGGEHFNIFAAGLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGI 642
           +FK+YDPVGGEHFNIFAAGLKTADR+VTVSHGYAWELKTSEGGWGLH IINE DWK  GI
Sbjct: 579 LFKMYDPVGGEHFNIFAAGLKTADRIVTVSHGYAWELKTSEGGWGLHNIINESDWKFRGI 638

Query: 643 VNGIDSKEWSPQYDIHLTSDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIG 702
           VNG+D+K+W+PQ+D +LTSDGYTNY+L+TL TGK QCKAALQ+ELGLP+REDVP+I FIG
Sbjct: 639 VNGVDTKDWNPQFDAYLTSDGYTNYNLKTLQTGKRQCKAALQRELGLPVREDVPIISFIG 698

Query: 703 RLDQQKGVDIIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKM 762
           RLD QKGVD+IAEAIPWM+  DVQLVMLGTGR DLEQML++FE QH DKIR WVGFSVKM
Sbjct: 699 RLDHQKGVDLIAEAIPWMMSHDVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGFSVKM 752

Query: 763 AHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWT 822
           AHRITAG DILLMPSRFEPCGLNQLYAM+YGT+PVVH VGGLRDTV PF+P++ESG+GWT
Sbjct: 759 AHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGGLRDTVQPFNPFDESGVGWT 752

Query: 823 FDSAEANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           FD AEANKL+ AL NCLLTY+ YKKSWEG+Q RGM+QDLSWD+AA++YEEVLVAAKYQW
Sbjct: 819 FDRAEANKLMAALWNCLLTYKDYKKSWEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW 752

BLAST of Lsi03G014970 vs. ExPASy Swiss-Prot
Match: Q6Z2T8 (Soluble starch synthase 2-2, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SSII-2 PE=2 SV=2)

HSP 1 Score: 802.0 bits (2070), Expect = 6.9e-231
Identity = 385/618 (62.30%), Postives = 455/618 (73.62%), Query Frame = 0

Query: 266 SKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTLKVDSDAVETASVD----VKPSPLAG 325
           + K  +D     + SK++    ++V   +P E       DA   +S D     +  PLAG
Sbjct: 140 ASKAEADGNAQAVESKAALDKKEDVGVAEPLEAKADAGGDAGAVSSADDSENKESGPLAG 199

Query: 326 VNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGLRK 385
            NVMNVI+VA+EC+P+ KTGGLGDV G+LPKALARRGHRVMV++PRY  YA  +D G+RK
Sbjct: 200 PNVMNVIVVASECSPFCKTGGLGDVVGALPKALARRGHRVMVVIPRYGEYAEAKDLGVRK 259

Query: 386 RYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIYGGDHLDILKRMVLFCKAAVEV 445
           RY+V GQD EVSYF  ++DGVDFVF++   F HR  +IYGG+  D+LKRM+LFCKAA   
Sbjct: 260 RYRVAGQDSEVSYFHAFIDGVDFVFLEAPPFRHRHNDIYGGERFDVLKRMILFCKAA--- 319

Query: 446 KLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYVPCGGVCYGDGNL 505
                                                    VEVPW+ PCGG  YGDGNL
Sbjct: 320 -----------------------------------------VEVPWFAPCGGSIYGDGNL 379

Query: 506 VFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLSLILWVRTPKEIN 565
           VF+ANDWHTA+LPVYLKAYYRDNG+M +TRS+LVIHNIAHQ                   
Sbjct: 380 VFIANDWHTALLPVYLKAYYRDNGLMQYTRSVLVIHNIAHQ------------------- 439

Query: 566 GRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGLKTADRVVTVSHGYAWELKTSE 625
           GRGPV+DF  +DLPEHYID F+LYDPVGGEH N+FAAGLK ADR VTVSHGY WE+KT +
Sbjct: 440 GRGPVDDFATMDLPEHYIDHFRLYDPVGGEHSNVFAAGLKMADRAVTVSHGYLWEIKTMD 499

Query: 626 GGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLETLHTGKPQCKAAL 685
           GGWGLH IIN  DWK+ GIVNGID  EW+P+ D HL SDGY NY+ ETL TGK QCK AL
Sbjct: 500 GGWGLHEIINHNDWKLQGIVNGIDMAEWNPEVDEHLQSDGYANYTFETLDTGKKQCKEAL 559

Query: 686 QKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRD 745
           Q++LGL +R+DVPLIGFIGRLD QKGVDII +A+PW+ GQDVQ+VMLGTGR DLE+MLR 
Sbjct: 560 QRQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWIAGQDVQVVMLGTGRPDLEEMLRR 619

Query: 746 FENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGG 805
           FE++H DK+RGWVGFSV++AHRITAG D+LLMPSRFEPCGLNQLYAM YGT+PVVH+VGG
Sbjct: 620 FESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG 679

Query: 806 LRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSW 865
           LRDTV PFDP+ ++GLGWTFD AEAN++I+ALG+CL TYR YK+SW GLQ RGM QDLSW
Sbjct: 680 LRDTVAPFDPFADTGLGWTFDRAEANRMIDALGHCLNTYRNYKESWRGLQARGMAQDLSW 694

Query: 866 DHAAEKYEEVLVAAKYQW 880
           DHAAE YE+VLV AKYQW
Sbjct: 740 DHAAELYEDVLVKAKYQW 694

BLAST of Lsi03G014970 vs. ExPASy Swiss-Prot
Match: Q7XE48 (Soluble starch synthase 2-1, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SSII-1 PE=2 SV=2)

HSP 1 Score: 798.9 bits (2062), Expect = 5.8e-230
Identity = 401/668 (60.03%), Postives = 481/668 (72.01%), Query Frame = 0

Query: 231 KSSEVLPDPK---DQLKGSSKKKSSDILQDSK--DQLKGSSKKNSS----DFVP------ 290
           KS EVL   +   +++  S +KK + I++DS   ++     +++SS    D VP      
Sbjct: 146 KSKEVLEIHRNLLEKISASERKKITSIIEDSSIYNEQDPFGQRDSSFYHLDEVPDDDEFS 205

Query: 291 ----AFLSSKSSYSTVKEVKDEQPSEPTLKVDSDAVETASVDVKPSPLAGVNVMNVIIVA 350
                +L  +   S V   +D +     +     +V   + D  PS  A V+++N+I+VA
Sbjct: 206 YDLQMYLDRRPDQSEVVATQDYEAQLSQISEMGQSVAEGTSD-DPSASAAVDLINIILVA 265

Query: 351 AECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGLRKRYKVDGQDHE 410
           AECAPWSKTGGLGDVAG+LPKALARRGHRVMV+VP Y NYA  Q  G  +RY+V GQD E
Sbjct: 266 AECAPWSKTGGLGDVAGALPKALARRGHRVMVVVPMYKNYAEPQQLGEPRRYQVAGQDME 325

Query: 411 VSYFQTYVDGVDFVFIDNNMFHHREQNIYGGDHLDILKRMVLFCKAAVEVKLANIEKESF 470
           V Y+  Y+DGVDFVFIDN +FHH E +IYGGD  DILKRMVL CKAA             
Sbjct: 326 VIYYHAYIDGVDFVFIDNPIFHHVENDIYGGDRTDILKRMVLLCKAA------------- 385

Query: 471 STLNLKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTA 530
                                          +EVPWYVPCGG CYGDGNLVF+ANDWHTA
Sbjct: 386 -------------------------------IEVPWYVPCGGYCYGDGNLVFLANDWHTA 445

Query: 531 MLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLSLILWVRTPKEINGRGPVNDFYH 590
           +LPVYLKAYY DNG M + RS+LVIHNIAHQ                   GRGP++DF +
Sbjct: 446 LLPVYLKAYYHDNGFMIYARSVLVIHNIAHQ-------------------GRGPLDDFSY 505

Query: 591 VDLPEHYIDIFKLYDPVGGEHFNIFAAGLKTADRVVTVSHGYAWELKTSEGGWGLHGIIN 650
           +DLP  Y+D+FKLYDP GG+H NIFAAG+K ADR++TVSHGYAWELKT+EGGWGLHGIIN
Sbjct: 506 LDLPVDYMDLFKLYDPFGGDHLNIFAAGIKAADRLLTVSHGYAWELKTAEGGWGLHGIIN 565

Query: 651 EIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLETLHTGKPQCKAALQKELGLPIRE 710
           E DWK  GIVNGID+ +W+P+ DIHL SDGYTNYSLET+  GK QCKAALQKELGLP+R 
Sbjct: 566 ESDWKFQGIVNGIDTTDWNPRCDIHLKSDGYTNYSLETVQAGKQQCKAALQKELGLPVRG 625

Query: 711 DVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIR 770
           DVP+I FIGRLD QKGVD+IAEA+PW+ GQDVQL+MLGTGRQDLE  LR  E+QH D++R
Sbjct: 626 DVPVIAFIGRLDHQKGVDLIAEAMPWIAGQDVQLIMLGTGRQDLEDTLRRLESQHYDRVR 685

Query: 771 GWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDP 830
           GWVGFS+++AHR+TAG DILLMPSRFEPCGLNQLYAM YGT+PVVH+VGGLRDTV  ++P
Sbjct: 686 GWVGFSIRLAHRMTAGADILLMPSRFEPCGLNQLYAMMYGTVPVVHAVGGLRDTVEHYNP 745

Query: 831 YNESGLGWTFDSAEANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEV 880
           Y ESGLGWTF+ AEAN+LI+ALG+CL TYR Y+ SWEGLQ+RGM QDLSWD+AA+ YEEV
Sbjct: 746 YEESGLGWTFEKAEANRLIDALGHCLNTYRNYRTSWEGLQKRGMMQDLSWDNAAKLYEEV 749

BLAST of Lsi03G014970 vs. ExPASy TrEMBL
Match: A0A5D3DQS9 (Starch synthase, chloroplastic/amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007900 PE=3 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 727/879 (82.71%), Postives = 763/879 (86.80%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTE+S+FVLSKNYHR+F  LK QNQRPAEGL FLGDKM++R GK PK
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSFSSLKPQNQRPAEGLSFLGDKMILRCGKLPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST VD
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST-VD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDSNEQ+DGLFP PDFPSRNDE  VDVLNGSSLSVSNGNVAVR+QLD   SSSSR 
Sbjct: 121 STEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDK+DEK VVDLKKKIYSADPKDQLKGSS+T YSDI                   L DPK
Sbjct: 181 SDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDI-------------------LADPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSSDILQDS+DQLKGSSKKNSSDFVPAFLSSKSS+S+VKE KD QPSEPTL
Sbjct: 241 DQLKGSSKSKSSDILQDSEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD VETA VDVKP PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDTVETAGVDVKPPPLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIY 420
           VMVIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+MFH+REQNIY
Sbjct: 361 VMVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNIY 420

Query: 421 GGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDF 480
           GGD L+ILKRMVLFCKAA                                          
Sbjct: 421 GGDRLEILKRMVLFCKAA------------------------------------------ 480

Query: 481 GSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIA 540
             +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNIA
Sbjct: 481 --IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNIA 540

Query: 541 HQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGL 600
           HQ                   GRGPV+DFYHVDLPEHY+D FKLYDP+GGEHFNIFAAGL
Sbjct: 541 HQ-------------------GRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAGL 600

Query: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSD 660
           KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTSD
Sbjct: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTSD 660

Query: 661 GYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720
           GYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG
Sbjct: 661 GYTNYSLETLQTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720

Query: 721 QDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780
           QDVQLVMLGTGRQDLEQMLRDFEN+HRDK+RGWVGFS+KMAHRITAGIDILLMPSRFEPC
Sbjct: 721 QDVQLVMLGTGRQDLEQMLRDFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEPC 780

Query: 781 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTY 840
           GLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLTY
Sbjct: 781 GLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLTY 796

Query: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           R+YKKSWEGLQ RGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 REYKKSWEGLQIRGMTQDLSWDHAAEKYEEVLVAAKYQW 796

BLAST of Lsi03G014970 vs. ExPASy TrEMBL
Match: A0A1S3B7Z7 (Starch synthase, chloroplastic/amyloplastic OS=Cucumis melo OX=3656 GN=LOC103486994 PE=3 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 727/879 (82.71%), Postives = 763/879 (86.80%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTE+S+FVLSKNYHR+F  LK QNQRPAEGL FLGDKM++R GK PK
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSFSSLKPQNQRPAEGLSFLGDKMILRCGKLPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST VD
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST-VD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDSNEQ+DGLFP PDFPSRNDE  VDVLNGSSLSVSNGNVAVR+QLD   SSSSR 
Sbjct: 121 STEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDK+DEK VVDLKKKIYSADPKDQLKGSS+T YSDI                   L DPK
Sbjct: 181 SDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDI-------------------LADPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSSDILQDS+DQLKGSSKKNSSDFVPAFLSSKSS+S+VKE KD QPSEPTL
Sbjct: 241 DQLKGSSKSKSSDILQDSEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD VETA VDVKP PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDTVETAGVDVKPPPLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIY 420
           VMVIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+MFH+REQNIY
Sbjct: 361 VMVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNIY 420

Query: 421 GGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDF 480
           GGD L+ILKRMVLFCKAA                                          
Sbjct: 421 GGDRLEILKRMVLFCKAA------------------------------------------ 480

Query: 481 GSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIA 540
             +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNIA
Sbjct: 481 --IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNIA 540

Query: 541 HQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGL 600
           HQ                   GRGPV+DFYHVDLPEHY+D FKLYDP+GGEHFNIFAAGL
Sbjct: 541 HQ-------------------GRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAGL 600

Query: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSD 660
           KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTSD
Sbjct: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTSD 660

Query: 661 GYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720
           GYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG
Sbjct: 661 GYTNYSLETLQTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720

Query: 721 QDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780
           QDVQLVMLGTGRQDLEQMLRDFEN+HRDK+RGWVGFS+KMAHRITAGIDILLMPSRFEPC
Sbjct: 721 QDVQLVMLGTGRQDLEQMLRDFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEPC 780

Query: 781 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTY 840
           GLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLTY
Sbjct: 781 GLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLTY 796

Query: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           R+YKKSWEGLQ RGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 REYKKSWEGLQIRGMTQDLSWDHAAEKYEEVLVAAKYQW 796

BLAST of Lsi03G014970 vs. ExPASy TrEMBL
Match: A0A5A7UCX8 (Starch synthase, chloroplastic/amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001580 PE=3 SV=1)

HSP 1 Score: 1417.1 bits (3667), Expect = 0.0e+00
Identity = 727/880 (82.61%), Postives = 763/880 (86.70%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTE+S+FVLSKNYHR+F  LK QNQRPAEGL FLGDKM++R GK PK
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSFSSLKPQNQRPAEGLSFLGDKMILRCGKLPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST VD
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST-VD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDSNEQ+DGLFP PDFPSRNDE  VDVLNGSSLSVSNGNVAVR+QLD   SSSSR 
Sbjct: 121 STEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDK+DEK VVDLKKKIYSADPKDQLKGSS+T YSDI                   L DPK
Sbjct: 181 SDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDI-------------------LADPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSSDILQDS+DQLKGSSKKNSSDFVPAFLSSKSS+S+VKE KD QPSEPTL
Sbjct: 241 DQLKGSSKSKSSDILQDSEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD VETA VDVKP PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDTVETAGVDVKPPPLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VM-VIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNI 420
           VM VIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+MFH+REQNI
Sbjct: 361 VMVVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNI 420

Query: 421 YGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPED 480
           YGGD L+ILKRMVLFCKAA                                         
Sbjct: 421 YGGDRLEILKRMVLFCKAA----------------------------------------- 480

Query: 481 FGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNI 540
              +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNI
Sbjct: 481 ---IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNI 540

Query: 541 AHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAG 600
           AHQ                   GRGPV+DFYHVDLPEHY+D FKLYDP+GGEHFNIFAAG
Sbjct: 541 AHQ-------------------GRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAG 600

Query: 601 LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTS 660
           LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTS
Sbjct: 601 LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTS 660

Query: 661 DGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV 720
           DGYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV
Sbjct: 661 DGYTNYSLETLQTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV 720

Query: 721 GQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEP 780
           GQDVQLVMLGTGRQDLEQMLRDFEN+HRDK+RGWVGFS+KMAHRITAGIDILLMPSRFEP
Sbjct: 721 GQDVQLVMLGTGRQDLEQMLRDFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEP 780

Query: 781 CGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLT 840
           CGLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLT
Sbjct: 781 CGLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLT 797

Query: 841 YRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           YR+YKKSWEGLQ RGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 YREYKKSWEGLQIRGMTQDLSWDHAAEKYEEVLVAAKYQW 797

BLAST of Lsi03G014970 vs. ExPASy TrEMBL
Match: A0A6J1F6A5 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita moschata OX=3662 GN=LOC111442471 PE=3 SV=1)

HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 703/881 (79.80%), Postives = 739/881 (83.88%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFC-FLKQQNQRPAEGLGFLGDKMVVRSGKWP 60
           M  +GSLPFTIEA TE+SM VLS  YHRT C  LKQQNQR AEG+GFL DKMV R+GKWP
Sbjct: 25  MVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQQNQRSAEGVGFLVDKMVGRNGKWP 84

Query: 61  KPVQAKAATSD-SSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTS 120
            P++A+AATSD +SGQR+DDPD+ IQATIEKSKKVLAMQQQLLEQIAERKKLV SIK TS
Sbjct: 85  TPLRARAATSDLNSGQRSDDPDQVIQATIEKSKKVLAMQQQLLEQIAERKKLVLSIKGTS 144

Query: 121 VDSKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSS 180
           +DSKEEQ+SNEQ+DG FPIPDFPSRNDE  VD LNGSSL VSN N+ VR+QLD   + SS
Sbjct: 145 LDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVPTFSS 204

Query: 181 RSSDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPD 240
           R SDKNDEK VVDLKK I SA PKDQLK SSK KYSD                   VLPD
Sbjct: 205 RPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYSD-------------------VLPD 264

Query: 241 PKDQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEP 300
            KDQLKGSSKK    +L DSK QLKGSSKK SSDFVPAFL+SKSSYS VKE K+ QPSEP
Sbjct: 265 RKDQLKGSSKK----VLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEP 324

Query: 301 TLKVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 360
           TLKVDSDA E+ SVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG
Sbjct: 325 TLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 384

Query: 361 HRVMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQN 420
           HRVMVIVPRYANYAGLQDTG+RKRYKVDGQD EV++FQTYVDGVDFVFIDNNMFHHREQN
Sbjct: 385 HRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQN 444

Query: 421 IYGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPE 480
           IYGGDH DILKRM  FCKAA                                        
Sbjct: 445 IYGGDHQDILKRMTFFCKAA---------------------------------------- 504

Query: 481 DFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHN 540
               VEVPW+VPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNG+M FTRSILVIHN
Sbjct: 505 ----VEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHN 564

Query: 541 IAHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAA 600
           IAHQ                   GRGPV+DFY VDLPEHY+D+FK+YDPVGGEHFNIFAA
Sbjct: 565 IAHQ-------------------GRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAA 624

Query: 601 GLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLT 660
           GLK ADRVVTVSHGYAWELKT EGGWGLHGIINEIDWKM GIVNGIDSKEWSPQYDIHLT
Sbjct: 625 GLKAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLT 684

Query: 661 SDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWM 720
           SDGYTNYS+ETL TGKPQCKAALQKELGLP+REDVPLIGFIGRLD QKGVDIIAEAIPWM
Sbjct: 685 SDGYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWM 744

Query: 721 VGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFE 780
           VGQDVQLV+LGTGRQDLEQMLRDFENQHRDK+RGWVGFSVKMAHRITAGIDILLMPSRFE
Sbjct: 745 VGQDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFE 804

Query: 781 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLL 840
           PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLL
Sbjct: 805 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLL 819

Query: 841 TYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           TYRQYKKSWEGLQRRGMTQDLSWDHAAE+YEEVLVAAKYQW
Sbjct: 865 TYRQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Lsi03G014970 vs. ExPASy TrEMBL
Match: A0A6J1J895 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita maxima OX=3661 GN=LOC111482246 PE=3 SV=1)

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 702/881 (79.68%), Postives = 738/881 (83.77%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTF-CFLKQQNQRPAEGLGFLGDKMVVRSGKWP 60
           M S+GSLPFTIEA TE+SM VLS  YHRT    LKQQNQR AEG+GFL DKMV RSGKWP
Sbjct: 25  MVSVGSLPFTIEAYTETSMIVLSNTYHRTVSSSLKQQNQRSAEGVGFLVDKMVGRSGKWP 84

Query: 61  KPVQAKAATSD-SSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTS 120
            P++A+AATSD +SGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAER+KLVSSIK TS
Sbjct: 85  TPLRARAATSDLNSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERRKLVSSIKGTS 144

Query: 121 VDSKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSS 180
           +DSKEEQ+SNEQ+DG FPIP FPS NDE  VD LNGSSL VSN N+ VR+QLD   + SS
Sbjct: 145 LDSKEEQNSNEQMDGSFPIPGFPSCNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVPTFSS 204

Query: 181 RSSDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPD 240
           R SDKNDEK VVDLKK I SA PKDQLK  SK KYSD                   VLPD
Sbjct: 205 RPSDKNDEKQVVDLKKIISSAGPKDQLKSISKIKYSD-------------------VLPD 264

Query: 241 PKDQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEP 300
            KDQLKGSSKK    IL DSK QLKGSSKK SSDFVPAFL+SKSSYS VKE K+ QPSEP
Sbjct: 265 RKDQLKGSSKK----ILPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEP 324

Query: 301 TLKVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 360
           TLKVDSDA E+ SVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG
Sbjct: 325 TLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 384

Query: 361 HRVMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQN 420
           HRVMVIVPRYANYAGLQDTG+RKRYKVDGQD EV++FQTYVDGVDFVFIDNNMFHHREQN
Sbjct: 385 HRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQN 444

Query: 421 IYGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPE 480
           IYGGDH DILKRM  FCKAA                                        
Sbjct: 445 IYGGDHQDILKRMTFFCKAA---------------------------------------- 504

Query: 481 DFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHN 540
               VEVPW+VPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNG+M FTRSILVIHN
Sbjct: 505 ----VEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHN 564

Query: 541 IAHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAA 600
           IAHQ                   GRGPV+DF+ VDLPEHY+D+FK+YDPVGGEHFNIFAA
Sbjct: 565 IAHQ-------------------GRGPVDDFHRVDLPEHYMDLFKMYDPVGGEHFNIFAA 624

Query: 601 GLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLT 660
           GLK ADRVVTVSHGYAWELKT EGGWGLHGIINEIDWKM GIVNGIDSKEWSPQYDIHLT
Sbjct: 625 GLKAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLT 684

Query: 661 SDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWM 720
           SDGYTNYS+ETL TGKPQCKAALQKELGLP+REDVPLIGFIGRLD QKGVDIIAEAIPWM
Sbjct: 685 SDGYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWM 744

Query: 721 VGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFE 780
           VGQDVQLV+LGTGRQDLEQMLRDFENQHRDK+RGWVGFSVKMAHRITAGIDILLMPSRFE
Sbjct: 745 VGQDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFE 804

Query: 781 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLL 840
           PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDP+NESG GWTFDSAEANKL++ALGNCLL
Sbjct: 805 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLVHALGNCLL 819

Query: 841 TYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           TYRQYKKSWEGLQRRGMTQDLSWDHAAE+YEEVLVAAKYQW
Sbjct: 865 TYRQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Lsi03G014970 vs. NCBI nr
Match: XP_038903759.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic [Benincasa hispida])

HSP 1 Score: 1483.4 bits (3839), Expect = 0.0e+00
Identity = 762/879 (86.69%), Postives = 784/879 (89.19%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTESSMFVLSKNY+R FC LKQQNQRP EGLGFLGDKMV R+GKWPK
Sbjct: 1   MASLGSLPFTIEAKTESSMFVLSKNYYRAFCPLKQQNQRPVEGLGFLGDKMVARNGKWPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           PV+AKAATSDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD
Sbjct: 61  PVKAKAATSDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           SKEEQDSN Q+DGLFP PDF SRNDES  DVLNGSSLSVSNG+VAV+EQLD   SSSSR 
Sbjct: 121 SKEEQDSNAQMDGLFPSPDFLSRNDESRFDVLNGSSLSVSNGSVAVKEQLDAVPSSSSRF 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDKNDEK V DLKK IYSADPKDQLKGSSKTK SDILPDLKDQLK SSKTKSSEV PDPK
Sbjct: 181 SDKNDEKQVGDLKKNIYSADPKDQLKGSSKTKSSDILPDLKDQLKGSSKTKSSEVSPDPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSS ILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKE KD  P++PTL
Sbjct: 241 DQLKGSSKTKSSVILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEGKD-PPNDPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           KVDSDAVETASVDVKP PLAG NVMNVIIVAAECAPWSKTGGLGDVAGSLPK+LARRGHR
Sbjct: 301 KVDSDAVETASVDVKPPPLAGANVMNVIIVAAECAPWSKTGGLGDVAGSLPKSLARRGHR 360

Query: 361 VMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIY 420
           VMVIVPRYANYAGLQDTG+RKRYKV+GQD EVSYFQTYVDGVDFVFIDN+MFHHREQNIY
Sbjct: 361 VMVIVPRYANYAGLQDTGVRKRYKVEGQDLEVSYFQTYVDGVDFVFIDNSMFHHREQNIY 420

Query: 421 GGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDF 480
           GGD LDILKRMVLFCKAA                                          
Sbjct: 421 GGDRLDILKRMVLFCKAA------------------------------------------ 480

Query: 481 GSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIA 540
             VEVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNIA
Sbjct: 481 --VEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNIA 540

Query: 541 HQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGL 600
           HQ                   GRGPV+DFYHVDLPEHYID+FKLYDPVGGEHFNIFAAGL
Sbjct: 541 HQ-------------------GRGPVDDFYHVDLPEHYIDLFKLYDPVGGEHFNIFAAGL 600

Query: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSD 660
           KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYD+HLTSD
Sbjct: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDVHLTSD 660

Query: 661 GYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720
           GYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG
Sbjct: 661 GYTNYSLETLDTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720

Query: 721 QDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780
           QDVQL++LGTGRQDLEQMLRDFENQHR+KIRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 721 QDVQLILLGTGRQDLEQMLRDFENQHRNKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780

Query: 781 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTY 840
           GLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEA+KLINALGNCLLTY
Sbjct: 781 GLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEASKLINALGNCLLTY 815

Query: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 815

BLAST of Lsi03G014970 vs. NCBI nr
Match: XP_011652235.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic [Cucumis sativus] >KAE8651213.1 hypothetical protein Csa_002583 [Cucumis sativus])

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 744/879 (84.64%), Postives = 780/879 (88.74%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTESSMFVLSKNY+R+FC LK QNQ+PAEGL  LGDKM+VRSGKWPK
Sbjct: 1   MASLGSLPFTIEAKTESSMFVLSKNYNRSFCSLKPQNQKPAEGLALLGDKMIVRSGKWPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTS D
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSAD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDS+EQ+DGL P PDFPSRNDES VDVLNGSSLSVSNGNVAVREQLD   SSSSR 
Sbjct: 121 STEKQDSSEQMDGLLPSPDFPSRNDESSVDVLNGSSLSVSNGNVAVREQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDKNDEK VVDLKKKIY+ADPKDQLKGSSKT YSDI+PD KDQL+ SSKTK SEVLPDPK
Sbjct: 181 SDKNDEKQVVDLKKKIYNADPKDQLKGSSKTTYSDIIPDPKDQLEGSSKTKFSEVLPDPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKG+ K KSS+ILQDSKDQLKGSSKK+SSDFVPAFLSSKSS+STVKE KD QPSEPTL
Sbjct: 241 DQLKGTLKTKSSEILQDSKDQLKGSSKKDSSDFVPAFLSSKSSHSTVKEGKDGQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD+VETA V VKP PLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDSVETAGVGVKPPPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIY 420
           VMVIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+ FH+REQNIY
Sbjct: 361 VMVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSTFHNREQNIY 420

Query: 421 GGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDF 480
           GGD LDILKRMVLFCKAA                                          
Sbjct: 421 GGDRLDILKRMVLFCKAA------------------------------------------ 480

Query: 481 GSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIA 540
             +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRD+GIM FTRSILVIHNIA
Sbjct: 481 --IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDSGIMKFTRSILVIHNIA 540

Query: 541 HQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGL 600
           HQ                   GRGPV+DFYHVDLPEHYID FKLYDPVGGEHFNIFAAGL
Sbjct: 541 HQ-------------------GRGPVDDFYHVDLPEHYIDHFKLYDPVGGEHFNIFAAGL 600

Query: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSD 660
           KTADRVVTVSHGYAWELKT+EGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTSD
Sbjct: 601 KTADRVVTVSHGYAWELKTTEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTSD 660

Query: 661 GYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720
           GYTNYSLETL TGK QCKAALQKELGLPIRED+PLIGFIGRLDQQKGVDIIAEAIPWMVG
Sbjct: 661 GYTNYSLETLQTGKAQCKAALQKELGLPIREDIPLIGFIGRLDQQKGVDIIAEAIPWMVG 720

Query: 721 QDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780
           Q+VQLVMLGTGRQDLEQMLRDFEN+HRDKIRGWVGFS+KMAHRITAGIDILLMPSRFEPC
Sbjct: 721 QNVQLVMLGTGRQDLEQMLRDFENKHRDKIRGWVGFSIKMAHRITAGIDILLMPSRFEPC 780

Query: 781 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTY 840
           GLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLTY
Sbjct: 781 GLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLTY 816

Query: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           +QYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 KQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 816

BLAST of Lsi03G014970 vs. NCBI nr
Match: XP_008443400.1 (PREDICTED: granule-bound starch synthase 2, chloroplastic/amyloplastic [Cucumis melo] >TYK25630.1 granule-bound starch synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 727/879 (82.71%), Postives = 763/879 (86.80%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTE+S+FVLSKNYHR+F  LK QNQRPAEGL FLGDKM++R GK PK
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSFSSLKPQNQRPAEGLSFLGDKMILRCGKLPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST VD
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST-VD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDSNEQ+DGLFP PDFPSRNDE  VDVLNGSSLSVSNGNVAVR+QLD   SSSSR 
Sbjct: 121 STEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDK+DEK VVDLKKKIYSADPKDQLKGSS+T YSDI                   L DPK
Sbjct: 181 SDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDI-------------------LADPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSSDILQDS+DQLKGSSKKNSSDFVPAFLSSKSS+S+VKE KD QPSEPTL
Sbjct: 241 DQLKGSSKSKSSDILQDSEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD VETA VDVKP PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDTVETAGVDVKPPPLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIY 420
           VMVIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+MFH+REQNIY
Sbjct: 361 VMVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNIY 420

Query: 421 GGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDF 480
           GGD L+ILKRMVLFCKAA                                          
Sbjct: 421 GGDRLEILKRMVLFCKAA------------------------------------------ 480

Query: 481 GSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIA 540
             +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNIA
Sbjct: 481 --IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNIA 540

Query: 541 HQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGL 600
           HQ                   GRGPV+DFYHVDLPEHY+D FKLYDP+GGEHFNIFAAGL
Sbjct: 541 HQ-------------------GRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAGL 600

Query: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSD 660
           KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTSD
Sbjct: 601 KTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTSD 660

Query: 661 GYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720
           GYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG
Sbjct: 661 GYTNYSLETLQTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVG 720

Query: 721 QDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPC 780
           QDVQLVMLGTGRQDLEQMLRDFEN+HRDK+RGWVGFS+KMAHRITAGIDILLMPSRFEPC
Sbjct: 721 QDVQLVMLGTGRQDLEQMLRDFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEPC 780

Query: 781 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTY 840
           GLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLTY
Sbjct: 781 GLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLTY 796

Query: 841 RQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           R+YKKSWEGLQ RGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 REYKKSWEGLQIRGMTQDLSWDHAAEKYEEVLVAAKYQW 796

BLAST of Lsi03G014970 vs. NCBI nr
Match: KAA0053773.1 (granule-bound starch synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1417.1 bits (3667), Expect = 0.0e+00
Identity = 727/880 (82.61%), Postives = 763/880 (86.70%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFCFLKQQNQRPAEGLGFLGDKMVVRSGKWPK 60
           MASLGSLPFTIEAKTE+S+FVLSKNYHR+F  LK QNQRPAEGL FLGDKM++R GK PK
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSFSSLKPQNQRPAEGLSFLGDKMILRCGKLPK 60

Query: 61  PVQAKAATSDSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVD 120
           P+QA+AA SDSSGQR+DDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST VD
Sbjct: 61  PMQARAAISDSSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST-VD 120

Query: 121 SKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRS 180
           S E+QDSNEQ+DGLFP PDFPSRNDE  VDVLNGSSLSVSNGNVAVR+QLD   SSSSR 
Sbjct: 121 STEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSSRP 180

Query: 181 SDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPK 240
           SDK+DEK VVDLKKKIYSADPKDQLKGSS+T YSDI                   L DPK
Sbjct: 181 SDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDI-------------------LADPK 240

Query: 241 DQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTL 300
           DQLKGSSK KSSDILQDS+DQLKGSSKKNSSDFVPAFLSSKSS+S+VKE KD QPSEPTL
Sbjct: 241 DQLKGSSKSKSSDILQDSEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTL 300

Query: 301 KVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360
           K DSD VETA VDVKP PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHR
Sbjct: 301 KDDSDTVETAGVDVKPPPLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHR 360

Query: 361 VM-VIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNI 420
           VM VIVPRYANYAGLQDTG+RKRYKV GQD+EVSYFQTYVDGVDFVFIDN+MFH+REQNI
Sbjct: 361 VMVVIVPRYANYAGLQDTGVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNI 420

Query: 421 YGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPED 480
           YGGD L+ILKRMVLFCKAA                                         
Sbjct: 421 YGGDRLEILKRMVLFCKAA----------------------------------------- 480

Query: 481 FGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNI 540
              +EVPWYVPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNGIM FTRSILVIHNI
Sbjct: 481 ---IEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNI 540

Query: 541 AHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAG 600
           AHQ                   GRGPV+DFYHVDLPEHY+D FKLYDP+GGEHFNIFAAG
Sbjct: 541 AHQ-------------------GRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAG 600

Query: 601 LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTS 660
           LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSP+YD+HLTS
Sbjct: 601 LKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTS 660

Query: 661 DGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV 720
           DGYTNYSLETL TGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV
Sbjct: 661 DGYTNYSLETLQTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMV 720

Query: 721 GQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEP 780
           GQDVQLVMLGTGRQDLEQMLRDFEN+HRDK+RGWVGFS+KMAHRITAGIDILLMPSRFEP
Sbjct: 721 GQDVQLVMLGTGRQDLEQMLRDFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEP 780

Query: 781 CGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLT 840
           CGLNQLYAMNYGTIPVVH VGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLLT
Sbjct: 781 CGLNQLYAMNYGTIPVVHRVGGLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLT 797

Query: 841 YRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           YR+YKKSWEGLQ RGMTQDLSWDHAAEKYEEVLVAAKYQW
Sbjct: 841 YREYKKSWEGLQIRGMTQDLSWDHAAEKYEEVLVAAKYQW 797

BLAST of Lsi03G014970 vs. NCBI nr
Match: KAG7017703.1 (Granule-bound starch synthase 2, chloroplastic/amyloplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 705/881 (80.02%), Postives = 741/881 (84.11%), Query Frame = 0

Query: 1   MASLGSLPFTIEAKTESSMFVLSKNYHRTFC-FLKQQNQRPAEGLGFLGDKMVVRSGKWP 60
           M  +GSLPFTIEA TE+SM VLS  YHRT C  LKQQNQR AEG+GFL DKMV R+GKWP
Sbjct: 25  MVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQQNQRSAEGVGFLVDKMVGRNGKWP 84

Query: 61  KPVQAKAATSD-SSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTS 120
            P++A+AATSD +SGQR+DDPD+AIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIK TS
Sbjct: 85  TPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKGTS 144

Query: 121 VDSKEEQDSNEQVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSS 180
           +DSKEEQ+SNEQ+DG FPIPDFPSRNDE  VD LNGSSL VSN N+ VR+QLD   + SS
Sbjct: 145 LDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVPTFSS 204

Query: 181 RSSDKNDEKPVVDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPD 240
           R SDKNDEK VVDLKK I SA PKDQLK SSK KYSD                   VLPD
Sbjct: 205 RPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYSD-------------------VLPD 264

Query: 241 PKDQLKGSSKKKSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEP 300
            KDQLKGSSKK    +L DSK QLKGSSKK SSDFVPAFL+SKSSYS VKE K+ QPSEP
Sbjct: 265 RKDQLKGSSKK----VLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEP 324

Query: 301 TLKVDSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 360
           TLKVDSDA E+ SVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG
Sbjct: 325 TLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 384

Query: 361 HRVMVIVPRYANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQN 420
           HRVMVIVPRYANYAGLQDTG+RKRYKVDGQD EV++FQTYVDGVDFVFIDNNMFHHREQN
Sbjct: 385 HRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQN 444

Query: 421 IYGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPE 480
           IYGGDH DILKRM  FCKAA                                        
Sbjct: 445 IYGGDHQDILKRMTFFCKAA---------------------------------------- 504

Query: 481 DFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHN 540
               VEVPW+VPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNG+M FTRSILVIHN
Sbjct: 505 ----VEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHN 564

Query: 541 IAHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAA 600
           IAHQ                   GRGPV+DFY VDLPEHY+D+FK+YDPVGGEHFNIFAA
Sbjct: 565 IAHQ-------------------GRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAA 624

Query: 601 GLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLT 660
           GLK ADRVVTVSHGYAWELKT EGGWGLHGIINEIDWKM GIVNGIDSKEWSPQYDIHLT
Sbjct: 625 GLKAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLT 684

Query: 661 SDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWM 720
           SDGYTNYS+ETL TGKPQCKAALQKELGLP+REDVPLIGFIGRLD QKGVDIIAEAIPWM
Sbjct: 685 SDGYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWM 744

Query: 721 VGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFE 780
           VGQDVQLV+LGTGRQDLEQMLRDFENQHRDK+RGWVGFSVKMAHRITAGIDILLMPSRFE
Sbjct: 745 VGQDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFE 804

Query: 781 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLL 840
           PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDP+NESG GWTFDSAEANKLI+ALGNCLL
Sbjct: 805 PCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLL 819

Query: 841 TYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           TYRQYKKSWEGLQRRGMTQDLSWDHAAE+YEEVLVAAKYQW
Sbjct: 865 TYRQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Lsi03G014970 vs. TAIR 10
Match: AT3G01180.1 (starch synthase 2 )

HSP 1 Score: 905.6 bits (2339), Expect = 3.1e-263
Identity = 485/810 (59.88%), Postives = 571/810 (70.49%), Query Frame = 0

Query: 70  DSSGQRNDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVDSKEEQDSNE 129
           ++SG  +D+P++A+QATI+KSKKVLAMQ+ LL QIAER+KLVSSIK ++ D  + + S++
Sbjct: 61  EASGSDDDEPEDALQATIDKSKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSK 120

Query: 130 QVDGLFPIPDFPSRNDESGVDVLNGSSLSVSNGNVAVREQLDVALSSSSRSSDKNDEKPV 189
           Q        +  +   E      NGS    + G  ++ ++ +    S S  S KN     
Sbjct: 121 QESASSVNANTDATKKEIMDGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKN----- 180

Query: 190 VDLKKKIYSADPKDQLKGSSKTKYSDILPDLKDQLKESSKTKSSEVLPDPKDQLKGSSKK 249
               +K  SA        +S  K SD+  + K      S   +S V P  K     +S +
Sbjct: 181 ----RKQSSASVISSSPVTSPQKPSDVATNGKPW----SSVVASSVDPPYKPSSVMTSPE 240

Query: 250 KSSDILQDSKDQLKGSSKKNSSDFVPAFLSSKSSYSTVKEVKDEQPSEPTLKVDSDAVET 309
           K+SD +       K  +    SD +P++L+     ST+K  K  + +       S+  E 
Sbjct: 241 KTSDPVTSPGKPSKSRAGAFWSDPLPSYLTKAPQTSTMKTEKYVEKTPDV--ASSETNEP 300

Query: 310 ASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYA 369
              + KP PLAG NVMNVI+VAAECAP+SKTGGLGDVAG+LPK+LARRGHRVMV+VPRYA
Sbjct: 301 GKDEEKPPPLAGANVMNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRYA 360

Query: 370 NYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQNIYGGDHLDILK 429
            YA  +D G+RKRYKV GQD EV YF  ++DGVDFVFID+  F H   NIYGG+ LDILK
Sbjct: 361 EYAEAKDLGVRKRYKVAGQDMEVMYFHAFIDGVDFVFIDSPEFRHLSNNIYGGNRLDILK 420

Query: 430 RMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYV 489
           RMVLFCKAA                                            VEVPWYV
Sbjct: 421 RMVLFCKAA--------------------------------------------VEVPWYV 480

Query: 490 PCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLS 549
           PCGGVCYGDGNL F+ANDWHTA+LPVYLKAYYRD+GIM +TRS+LVIHNIAHQ       
Sbjct: 481 PCGGVCYGDGNLAFIANDWHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQ------- 540

Query: 550 LILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVGGEHFNIFAAGLKTADRVVTV 609
                       GRGPV+DF +VDLP HY+D FKLYDPVGGEHFNIFAAGLK ADRV+TV
Sbjct: 541 ------------GRGPVDDFSYVDLPSHYLDSFKLYDPVGGEHFNIFAAGLKAADRVLTV 600

Query: 610 SHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLET 669
           SHGY+WE+KT EGGWGLH IINE DWK  GIVNGID++EW+P++D +L SD YTNYSLE 
Sbjct: 601 SHGYSWEVKTLEGGWGLHNIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDYTNYSLEN 660

Query: 670 LHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLG 729
           LH GKPQCKAALQKELGLP+R DVPLIGFIGRLD QKGVD+IAEA+PWM+ QDVQLVMLG
Sbjct: 661 LHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMMSQDVQLVMLG 720

Query: 730 TGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMN 789
           TGR DLE++LR  E+Q+RDK RGWVGFSVK AHRITAG DILLMPSRFEPCGLNQLYAMN
Sbjct: 721 TGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMN 780

Query: 790 YGTIPVVHSVGGLRDTVIPFDPYNESGLGWTFDSAEANKLINALGNCLLTYRQYKKSWEG 849
           YGTIPVVH+VGGLRDTV  FDPY+E+GLGWTFDSAEA KLI+ALGNCLLTYR+YK+SWEG
Sbjct: 781 YGTIPVVHAVGGLRDTVQQFDPYSETGLGWTFDSAEAGKLIHALGNCLLTYREYKESWEG 792

Query: 850 LQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
           LQRRGMTQDLSWD+AAEKYEEVLVAAKY W
Sbjct: 841 LQRRGMTQDLSWDNAAEKYEEVLVAAKYHW 792

BLAST of Lsi03G014970 vs. TAIR 10
Match: AT5G24300.1 (Glycogen/starch synthases, ADP-glucose type )

HSP 1 Score: 456.1 bits (1172), Expect = 6.6e-128
Identity = 266/594 (44.78%), Postives = 340/594 (57.24%), Query Frame = 0

Query: 306 AVETASVDVK-PSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVI 365
           AVE  + +V  PS  A V V N++ V +E AP+SKTGGLGDV GSLP ALA RGHRVMVI
Sbjct: 123 AVEEGAAEVGIPSGKAEV-VNNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVI 182

Query: 366 VPRY-------ANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQ 425
            PRY        NYA  +D G+R      G   EVS++  Y DGVD+VF+D+  + HR  
Sbjct: 183 SPRYLNGTAADKNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWVFVDHKSY-HRPG 242

Query: 426 NIYG---GDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVIT 485
           N YG   G   D   R  L C AA                                    
Sbjct: 243 NPYGDSKGAFGDNQFRFTLLCHAA------------------------------------ 302

Query: 486 VDPEDFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSIL 545
                    E P  +P GG  YG+ +L F+ NDWH  ++P+ L A YR  G+    RSIL
Sbjct: 303 --------CEAPLVLPLGGFTYGEKSL-FLVNDWHAGLVPILLAAKYRPYGVYKDARSIL 362

Query: 546 VIHNIAHQ-----VCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVG 605
           +IHN+AHQ       Y+ L L      P E    G V   +      H +D         
Sbjct: 363 IIHNLAHQGVEPAATYTNLGL------PSE--WYGAVGWVFPTWARTHALDT-------- 422

Query: 606 GEHFNIFAAGLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEW 665
           GE  N+    + T+DR++TVS GYAWE+ T EGG+GL  +++     + GI NGI+  EW
Sbjct: 423 GEAVNVLKGAIVTSDRIITVSQGYAWEITTVEGGYGLQDLLSSRKSVINGITNGINVDEW 482

Query: 666 SPQYDIHLTSDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVD 725
           +P  D H+      +YS + + + K +CK ALQKELGLPIR + P+IGFIGRLD QKG+D
Sbjct: 483 NPSTDEHIP----FHYSADDV-SEKIKCKMALQKELGLPIRPECPMIGFIGRLDYQKGID 542

Query: 726 IIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGID 785
           +I  A P ++  D+Q VMLG+G    E  +R  E  +RDK RGWVGF+V ++HRITAG D
Sbjct: 543 LIQTAGPDLMVDDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNVPISHRITAGCD 602

Query: 786 ILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNE----SGLGWTFDSAE 845
           ILLMPSRFEPCGLNQLYAM YGTIPVVH  GGLRDTV  F+PY E    +G GW F    
Sbjct: 603 ILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGGAGTGTGWVFTPLS 643

Query: 846 ANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
            + +++AL     TYR+YK+SWEGL RRGMT++ SW++AA +YE+V     +QW
Sbjct: 663 KDSMVSALRLAAATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQV-----FQW 643

BLAST of Lsi03G014970 vs. TAIR 10
Match: AT5G24300.2 (Glycogen/starch synthases, ADP-glucose type )

HSP 1 Score: 456.1 bits (1172), Expect = 6.6e-128
Identity = 266/594 (44.78%), Postives = 340/594 (57.24%), Query Frame = 0

Query: 306 AVETASVDVK-PSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVI 365
           AVE  + +V  PS  A V V N++ V +E AP+SKTGGLGDV GSLP ALA RGHRVMVI
Sbjct: 123 AVEEGAAEVGIPSGKAEV-VNNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVI 182

Query: 366 VPRY-------ANYAGLQDTGLRKRYKVDGQDHEVSYFQTYVDGVDFVFIDNNMFHHREQ 425
            PRY        NYA  +D G+R      G   EVS++  Y DGVD+VF+D+  + HR  
Sbjct: 183 SPRYLNGTAADKNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWVFVDHKSY-HRPG 242

Query: 426 NIYG---GDHLDILKRMVLFCKAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVIT 485
           N YG   G   D   R  L C AA                                    
Sbjct: 243 NPYGDSKGAFGDNQFRFTLLCHAA------------------------------------ 302

Query: 486 VDPEDFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSIL 545
                    E P  +P GG  YG+ +L F+ NDWH  ++P+ L A YR  G+    RSIL
Sbjct: 303 --------CEAPLVLPLGGFTYGEKSL-FLVNDWHAGLVPILLAAKYRPYGVYKDARSIL 362

Query: 546 VIHNIAHQ-----VCYSCLSLILWVRTPKEINGRGPVNDFYHVDLPEHYIDIFKLYDPVG 605
           +IHN+AHQ       Y+ L L      P E    G V   +      H +D         
Sbjct: 363 IIHNLAHQGVEPAATYTNLGL------PSE--WYGAVGWVFPTWARTHALDT-------- 422

Query: 606 GEHFNIFAAGLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDWKMMGIVNGIDSKEW 665
           GE  N+    + T+DR++TVS GYAWE+ T EGG+GL  +++     + GI NGI+  EW
Sbjct: 423 GEAVNVLKGAIVTSDRIITVSQGYAWEITTVEGGYGLQDLLSSRKSVINGITNGINVDEW 482

Query: 666 SPQYDIHLTSDGYTNYSLETLHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVD 725
           +P  D H+      +YS + + + K +CK ALQKELGLPIR + P+IGFIGRLD QKG+D
Sbjct: 483 NPSTDEHIP----FHYSADDV-SEKIKCKMALQKELGLPIRPECPMIGFIGRLDYQKGID 542

Query: 726 IIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGID 785
           +I  A P ++  D+Q VMLG+G    E  +R  E  +RDK RGWVGF+V ++HRITAG D
Sbjct: 543 LIQTAGPDLMVDDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNVPISHRITAGCD 602

Query: 786 ILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPYNE----SGLGWTFDSAE 845
           ILLMPSRFEPCGLNQLYAM YGTIPVVH  GGLRDTV  F+PY E    +G GW F    
Sbjct: 603 ILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGGAGTGTGWVFTPLS 643

Query: 846 ANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAEKYEEVLVAAKYQW 880
            + +++AL     TYR+YK+SWEGL RRGMT++ SW++AA +YE+V     +QW
Sbjct: 663 KDSMVSALRLAAATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQV-----FQW 643

BLAST of Lsi03G014970 vs. TAIR 10
Match: AT1G32900.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 328.2 bits (840), Expect = 2.1e-89
Identity = 212/570 (37.19%), Postives = 291/570 (51.05%), Query Frame = 0

Query: 325 MNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGLRKRYK 384
           M+VI + AE  PWSKTGGLGDV G LP ALA RGHRVM I PRY  Y    DT +  + K
Sbjct: 84  MSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRYDQYKDAWDTCVVVQIK 143

Query: 385 VDGQDHEVSYFQTYVDGVDFVFIDNNMFHHR-----EQNIYGG----DHLDILKRMVLFC 444
           V  +   V +F  Y  GVD VF+D+ +F  +        IYG     D+ D   R  L C
Sbjct: 144 VGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIYGPITGVDYNDNQLRFSLLC 203

Query: 445 KAAVEVKLANIEKESFSTLNLKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYVPCGGVC 504
           +AA+E                  VLN N  +   G                         
Sbjct: 204 QAALEAPQ---------------VLNLNSSKYFSGP------------------------ 263

Query: 505 YGDGNLVFVANDWHTAMLPVYLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLSLILWVR 564
           YG+ ++VFVANDWHTA+LP YLK+ Y+  G+    + +  IHNIA+Q             
Sbjct: 264 YGE-DVVFVANDWHTALLPCYLKSMYQSRGVYMNAKVVFCIHNIAYQ------------- 323

Query: 565 TPKEINGRGPVNDFYHVDLPEHYIDIFKLYD----PVGGEHFNIFAAGLKTADRVVTVSH 624
                 GR   +D+  ++LP  +   F   D    PV G   N   A +  A RV+TVS 
Sbjct: 324 ------GRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGRKINWMKAAILEAHRVLTVSP 383

Query: 625 GYAWELKTS-EGGWGLHGIINEIDWKMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLETL 684
            YA EL +  + G  LH  +      + GI+NG+D +EW+P  D ++       Y + T+
Sbjct: 384 YYAQELISGVDRGVELHKYLRM--KTVSGIINGMDVQEWNPSTDKYID----IKYDITTV 443

Query: 685 HTGKPQCKAALQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLGT 744
              KP  K ALQ  +GLP+  DVP+IGFIGRL++QKG DI+ EAI   +G +VQ+V+LGT
Sbjct: 444 TDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFMGLNVQMVILGT 503

Query: 745 GRQDLEQMLRDFENQHRDKIRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNY 804
           G++ +E  + + E +   K  G   F+V +AH ITAG D +++PSRFEPCGL QL+AM Y
Sbjct: 504 GKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMRY 563

Query: 805 GTIPVVHSVGGLRDTVIPFDPYNESGLG------WTFDSAEANKLINALGNCLLTYRQYK 864
           GT+P+V S GGL DTV   D Y    +G         D  +      A+   +  Y    
Sbjct: 564 GTVPIVASTGGLVDTV--KDGYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYG--T 584

Query: 865 KSWEGLQRRGMTQDLSWDHAAEKYEEVLVA 875
            + + + +  M QD SW   A  +E+VL++
Sbjct: 624 SAMQEMVKNCMDQDFSWKGPARLWEKVLLS 584

BLAST of Lsi03G014970 vs. TAIR 10
Match: AT4G18240.1 (starch synthase 4 )

HSP 1 Score: 179.1 bits (453), Expect = 1.5e-44
Identity = 224/845 (26.51%), Postives = 365/845 (43.20%), Query Frame = 0

Query: 87   IEKSKKVLAMQQQLLEQIAERKKLVSSIKSTSVDSKEEQDSNEQV-------DGL-FPIP 146
            IE    VLA+ ++L     E   L + I+    +    +D+ E+V        GL   + 
Sbjct: 269  IESDGYVLALSKELETLKLENLSLRNDIEMLKSELDSVKDTGERVVVLEKECSGLESSVK 328

Query: 147  DFPSRNDESGVDVLNGSSLSVSNGNV-AVREQLDVALSSSSRSSDKNDEKPVVDLKKKIY 206
            D  S+   S  DV   S+L +   ++ A  E L + L  ++    K  E+ V+ L++   
Sbjct: 329  DLESKLSVSQEDVSQLSTLKIECTDLWAKVETLQLLLDRAT----KQAEQAVIVLQQNQD 388

Query: 207  SADPKDQLKGSSK------------TKYSDILPDLKDQLKESSKTKSSEVLPDPKDQLKG 266
              +  D+++ S K             +Y++++      L+E  +   +E+      QL  
Sbjct: 389  LRNKVDKIEESLKEANVYKESSEKIQQYNELMQHKVTLLEERLEKSDAEIF--SYVQLYQ 448

Query: 267  SSKKKSSDILQDSKDQLKGSSKKNSSDFVP------------AFLSSKSSYSTVKEV--- 326
             S K+  + L+  K++ K  S+    D +P             +L  K   S   ++   
Sbjct: 449  ESIKEFQETLESLKEESKKKSRDEPVDDMPWDYWSRLLLTVDGWLLEKKIASNDADLLRD 508

Query: 327  ----KDEQPSEPTLKV-DSDAVETASVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGD 386
                KD +  +  + V D +  +  S  +K       + + V+ +AAE AP +K GGLGD
Sbjct: 509  MVWKKDRRIHDTYIDVKDKNERDAISAFLKLVSSPTSSGLYVVHIAAEMAPVAKVGGLGD 568

Query: 387  VAGSLPKALARRGHRVMVIVPRY--ANYAGLQDTGLRKRYKV-----DGQDHEVSYFQTY 446
            V   L KAL R+GH V +I+P+Y    Y  ++D  LR    V     DG+ ++   +   
Sbjct: 569  VVAGLGKALQRKGHLVEIILPKYDCMQYDRVRD--LRALDTVVESYFDGKLYKNKIWIGT 628

Query: 447  VDGVDFVFID---NNMFHHREQNIYGGDHLDILKRMVLFCKAAVEVKLANIEKESFSTLN 506
            V+G+   FI+    + F  R Q  YG    D  +R   F +AA+E+ L + +K       
Sbjct: 629  VEGLPVHFIEPQHPSKFFWRGQ-FYG--EQDDFRRFSYFSRAALELLLQSGKK------- 688

Query: 507  LKFVLNFNEDRLQKGRVITVDPEDFGSVEVPWYVPCGGVCYGDGNLVFVANDWHTAMLPV 566
                                          P  + C              +DW TA +  
Sbjct: 689  ------------------------------PDIIHC--------------HDWQTAFVAP 748

Query: 567  YLKAYYRDNGIMTFTRSILVIHNIAHQVCYSCLSLILWVRTPKEINGRGPVNDFYHVDLP 626
                 Y   G+ +  R     HN  +Q   S            E+   G   D   ++ P
Sbjct: 749  LYWDLYAPKGLDS-ARICFTCHNFEYQGTASA----------SELGSCG--LDVNQLNRP 808

Query: 627  EHYIDIFKLYDPVGGEHFNIFAAGLKTADRVVTVSHGYAWELKTSEGGWGLHGIINEIDW 686
            +      ++ D   G+  N     +  ++ V TVS  YA E++T+EGG GLH  +N    
Sbjct: 809  D------RMQDHSSGDRVNPVKGAIIFSNIVTTVSPTYAQEVRTAEGGKGLHSTLNFHSK 868

Query: 687  KMMGIVNGIDSKEWSPQYDIHLTSDGYTNYSLETLHTGKPQCKAALQKELGLPIRED-VP 746
            K +GI+NGID+  W+P  D  L +     ++ + L  GK + K AL+K+LGL   E   P
Sbjct: 869  KFIGILNGIDTDSWNPATDPFLKA----QFNAKDLQ-GKEENKHALRKQLGLSSAESRRP 928

Query: 747  LIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLGTGRQDLEQMLRDFEN-----QHRDK 806
            L+G I RL  QKGV +I  AI   +    Q V+LG+    +  + R+FE      +  D 
Sbjct: 929  LVGCITRLVPQKGVHLIRHAIYRTLELGGQFVLLGS--SPVPHIQREFEGIEQQFKSHDH 988

Query: 807  IRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVIPF 866
            +R  + +   ++H I A  D+ ++PS FEPCGL Q+ AM YG+IP+    GGL D+V   
Sbjct: 989  VRLLLKYDEALSHTIYAASDLFIIPSIFEPCGLTQMIAMRYGSIPIARKTGGLNDSVFDI 1025

Query: 867  DP---YNESGLGWTFDSAEANKLINALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAE 872
            D      +   G+TF +A+      AL      Y++ ++ W  L  + M+ D SW  +A 
Sbjct: 1049 DDDTIPTQFQNGFTFQTADEQGFNYALERAFNHYKKDEEKWMRLVEKVMSIDFSWGSSAT 1025