Lsi03G014920 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014920
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease T(2)
Location	chr03: 25946374 .. 25946658 (+)
RNA-Seq Expression	Lsi03G014920
Synteny	Lsi03G014920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAGGTACTTCCAAACAGCGATAAACATGAGGCACAGTATCGACCTTCTTAGTGTCATGAGAACTGGCGGAGTTGTACCAAACGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTCATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGTTGTAAAACAGCACAAAATGGCCAAGTCTTATTGACAGAGATTGTTATGTGCTTTGATGACGATGGTATCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTCGAAGCTTTATTTTTTGA

mRNA sequence

ATGGCAAGGTACTTCCAAACAGCGATAAACATGAGGCACAGTATCGACCTTCTTAGTGTCATGAGAACTGGCGGAGTTGTACCAAACGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTCATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGTTGTAAAACAGCACAAAATGGCCAAGTCTTATTGACAGAGATTGTTATGTGCTTTGATGACGATGGTATCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTCGAAGCTTTATTTTTTGA

Coding sequence (CDS)

ATGGCAAGGTACTTCCAAACAGCGATAAACATGAGGCACAGTATCGACCTTCTTAGTGTCATGAGAACTGGCGGAGTTGTACCAAACGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTCATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGTTGTAAAACAGCACAAAATGGCCAAGTCTTATTGACAGAGATTGTTATGTGCTTTGATGACGATGGTATCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTCGAAGCTTTATTTTTTGA

Protein sequence

MARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQVLLTEIVMCFDDDGITLIHCNKARSNCARSFIF

Homology

[Close]

BLAST of Lsi03G014920 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 9.5e-14
Identity = 36/94 (38.30%), Postives = 57/94 (60.64%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A YF+ A++MR++ D++  +R     PNG +K++Q ++  + + FG+ P LRC+T    +
Sbjct: 100 AAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTK 159

Query: 62  V-LLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           V  L ++V CF  DG TLI C   R  C  +FIF
Sbjct: 160 VSYLVQVVACFAQDGSTLIDC--TRDTCGANFIF 191

[Close]

BLAST of Lsi03G014920 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 2.7e-08
Identity = 25/78 (32.05%), Postives = 45/78 (57.69%), Query Frame = 0


Query: 4   YFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQVL 63
           YF+ A+++++ IDLLS+++   + P+G S     + + I S  G  P ++C   Q+G   
Sbjct: 136 YFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQ 195

Query: 64  LTEIVMCFDDDGITLIHC 82
           L ++ +C D  G +LI C
Sbjct: 196 LYQVYICVDGSGSSLIEC 213

[Close]

BLAST of Lsi03G014920 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 5.1e-07
Identity = 24/78 (30.77%), Postives = 39/78 (50.00%), Query Frame = 0


Query: 4   YFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQVL 63
           YFQTA+N++   +LL  +   G+ P+G S + + +   I    G  P + C    +G   
Sbjct: 136 YFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQ 195

Query: 64  LTEIVMCFDDDGITLIHC 82
           L ++ +C D  G  LI C
Sbjct: 196 LYQVYLCVDRSGSGLIEC 213

[Close]

BLAST of Lsi03G014920 vs. ExPASy Swiss-Prot
Match: Q38717 (Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 4.3e-06
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 4   YFQTAINMRHSIDLLSVMRTGGVVPNGASK-AKQRVESVISSHFGRDPILRCKTAQNGQV 63
           YF  A+ ++  +D+L+++      P      +  RV + IS   G+ PIL+C      Q 
Sbjct: 138 YFLKALELKDRLDVLTILENNNFNPGTPQPFSVLRVFNTISRAIGKTPILKC-----AQS 197

Query: 64  LLTEIVMCFDDDGITLIHCNKAR 86
            L E+V+C D++G +++HC ++R
Sbjct: 198 YLKEVVICVDNNGASVVHCPRSR 215

[Close]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL
Match: A0A5A7UJY4 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007930 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-41
Identity = 82/93 (88.17%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRHSIDLLSV+RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCKTA NGQ
Sbjct: 54  AKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQ 113

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIV+CFDDDG+TLI+CNKARSNCARSFIF
Sbjct: 114 VLLTEIVICFDDDGVTLINCNKARSNCARSFIF 146

[Close]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-41
Identity = 82/93 (88.17%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRHSIDLLSV+RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCKTA NGQ
Sbjct: 124 AKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQ 183

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIV+CFDDDG+TLI+CNKARSNCARSFIF
Sbjct: 184 VLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 6.2e-40
Identity = 81/93 (87.10%), Postives = 87/93 (93.55%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRH IDLLSV+RTGGV PNGASKAKQRVE+ ISSHFG+DPILRCK A NGQ
Sbjct: 125 AKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQ 184

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIVMCFDDDG+TLI+CNKARSNCA SFIF
Sbjct: 185 VLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL
Match: A0A6J1J1Y7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-33
Identity = 69/93 (74.19%), Postives = 80/93 (86.02%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A YFQT+IN+RH+IDLLS +R  GVVPNGASK+KQRVE+ I  HF  +P+LRCKTA NGQ
Sbjct: 123 AAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQ 182

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIVMC DDDG+TLI+CN A+SNCA SFIF
Sbjct: 183 VLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL
Match: A0A6J1F776 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-33
Identity = 69/93 (74.19%), Postives = 80/93 (86.02%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A YFQT+IN+RH+IDLLS +R  GVVPNGASK+KQRVE+ I  HF  +P+LRCKTA NGQ
Sbjct: 123 AAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQ 182

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIVMC DDDG+TLI+CN A+SNCA SFIF
Sbjct: 183 VLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of Lsi03G014920 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 181.0 bits (458), Expect = 4.7e-42
Identity = 84/93 (90.32%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A YFQTAINMRHSIDLLSVMRTGGV+PNGASKAKQRVESVISSHFG+DPILRCKTA NGQ
Sbjct: 124 AAYFQTAINMRHSIDLLSVMRTGGVIPNGASKAKQRVESVISSHFGKDPILRCKTASNGQ 183

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTE+VMCFDDDG+TL++CNKARSNCA SFIF
Sbjct: 184 VLLTEVVMCFDDDGVTLLNCNKARSNCATSFIF 216

[Close]

BLAST of Lsi03G014920 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 177.9 bits (450), Expect = 4.0e-41
Identity = 82/93 (88.17%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRHSIDLLSV+RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCKTA NGQ
Sbjct: 124 AKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQ 183

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIV+CFDDDG+TLI+CNKARSNCARSFIF
Sbjct: 184 VLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of Lsi03G014920 vs. NCBI nr
Match: KAA0053769.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25633.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 177.9 bits (450), Expect = 4.0e-41
Identity = 82/93 (88.17%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRHSIDLLSV+RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCKTA NGQ
Sbjct: 54  AKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQ 113

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIV+CFDDDG+TLI+CNKARSNCARSFIF
Sbjct: 114 VLLTEIVICFDDDGVTLINCNKARSNCARSFIF 146

[Close]

BLAST of Lsi03G014920 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 172.9 bits (437), Expect = 1.3e-39
Identity = 81/93 (87.10%), Postives = 87/93 (93.55%), Query Frame = 0


Query: 2   ARYFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQ 61
           A+YFQTAINMRH IDLLSV+RTGGV PNGASKAKQRVE+ ISSHFG+DPILRCK A NGQ
Sbjct: 125 AKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQ 184

Query: 62  VLLTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           VLLTEIVMCFDDDG+TLI+CNKARSNCA SFIF
Sbjct: 185 VLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Lsi03G014920 vs. NCBI nr
Match: XP_023528964.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 152.1 bits (383), Expect = 2.3e-33
Identity = 69/91 (75.82%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 4   YFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQVL 63
           YFQT+IN+RH+I+LLS +R  GVVPNGASK+KQRVES I SHF ++P+LRCKTA NGQVL
Sbjct: 125 YFQTSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKNPVLRCKTASNGQVL 184

Query: 64  LTEIVMCFDDDGITLIHCNKARSNCARSFIF 95
           LTEIVMC DDDG+TLI+CN A+SNCA SFIF
Sbjct: 185 LTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of Lsi03G014920 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 55.1 bits (131), Expect = 3.6e-08
Identity = 24/78 (30.77%), Postives = 39/78 (50.00%), Query Frame = 0


Query: 4   YFQTAINMRHSIDLLSVMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKTAQNGQVL 63
           YFQTA+N++   +LL  +   G+ P+G S + + +   I    G  P + C    +G   
Sbjct: 136 YFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQ 195

Query: 64  LTEIVMCFDDDGITLIHC 82
           L ++ +C D  G  LI C
Sbjct: 196 LYQVYLCVDRSGSGLIEC 213

The following BLAST results are available for this feature:

BLAST of Lsi03G014920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	9.5e-14	38.30	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	2.7e-08	32.05	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42813	5.1e-07	30.77	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
Q38717	4.3e-06	30.12	Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1	[more]

BLAST of Lsi03G014920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UJY4	1.9e-41	88.17	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A1S3B8Q3	1.9e-41	88.17	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A0A0LC37	6.2e-40	87.10	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1J1Y7	1.5e-33	74.19	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1	[more]
A0A6J1F776	1.5e-33	74.19	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1	[more]

BLAST of Lsi03G014920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905534.1	4.7e-42	90.32	ribonuclease MC-like [Benincasa hispida]	[more]
XP_008443402.1	4.0e-41	88.17	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
KAA0053769.1	4.0e-41	88.17	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25633.1 ribonuclease MC-like...	[more]
XP_004147499.1	1.3e-39	87.10	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_023528964.1	2.3e-33	75.82	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G014920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	3.6e-08	30.77	ribonuclease 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 3..81 e-value: 2.3E-11 score: 44.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 3..94
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 1..94 e-value: 2.3E-17 score: 65.7
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 3..86
None	No IPR available	PANTHER	PTHR11240:SF57	OS09G0538000 PROTEIN	coord: 3..94

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014920.1	Lsi03G014920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding