Lsi03G012770 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G012770
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cytochrome P450 87A3-like
Location	chr03: 23557615 .. 23562649 (-)
RNA-Seq Expression	Lsi03G012770
Synteny	Lsi03G012770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAGCAAAATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGACAATGTTTTATTTTTTTTTTCCTTTTTGTATATTAAGAAATTAAACCATCTATATTATTCTTTCTTCTTTTTTTTTTTTTTTTGAATTTTGGTATTTTTGTTTCAGGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATGTAAATTTATATTGAAAAAAAAAAAACTTGTTTTAATTATTTGACATGTTCAATCAAGGTTTTAATTTTTCTTTTTTACCTTATTAATATTAATTTGGTTCCATGCAGATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGGTAACTTTTTATTTATTTATTTAGTTGTGGTTCCTTAAATTACTTGTGTCAAAAAAAAAAAAAAAAACTTGGTCGTTTTAGGGTTTTTCTTTCAAAATTAACCTATGAAAAACGGAAGATGAGAGTCTGATAGAAATTTATTTTTAGTAGGTTAAAGAATTATAAATTTTTTATTTTTAAAAGGCTAAATAGCACAGTTTGAAGTTACGTGCCACCCTAAATTTTTAAAAAAAAAATCATTTTCACCTTTCAATTTCGAAATTATTCTAATAATATATGATTTTAGAGGGCCTTTTATTTATTTATTTATTTAAGATGTACGAAGGTAAAAACGATTAGTGTGTAGTTGATTGTACGAATTGATCATATTATTTTCTAACTCATGTTTCAACTTTGTATAGGAAATTAAAAATATAATTCTAAATGATGTGATGTATGTTAGTCCAATTAATGTTACACATTAGTGTTGTTGTACTGTCAATTTCCACTCATCGTTATTTTTTTTTTGGATTGTTTTTGGGAATAATTTAAGAGTTCAAGAATTAAAAAATAATAATAAAATATAAAAAATCTCGATCAACAATAAAATGTACAAACTCAAATGACGATAAGTACATCTTGGATGCAAGATGCAACTATCTTATTGCAATTCTTTCTTCTTTCTTCTTTTTCTTAAACATCTTTGAATTTTTTATGTTGTATTGTTAGAGAAGGAAAGGGGGTAATTTATGATATACACTCTAGCCACTTGGTTCCCAGACATCCTCAATTTTTATAGGGGAACATTTAGGGACTCATTTGATAACGTTTCTATTTTCTATTTCCTGTTTTTGTTTCTCATTTTTTAAGAAGCTGACTTGTTTGATAATCGTTCTTGTTTCTTGTGTTTAGAATTTGAGAAACGTTTCTAAAAATGGGGCCAAATTTGAGAAACTGTTCCATTTCTATTTTATATAATGTTGTTTCTTTTATTTATCAAAACTCATTTTTGACATATTGGTAGTCATGCATGTCTATACTACTGTTTTACCTTAAACGGACCCTAACGTTTCCGTTTCTTGTTTCTCATTTTTTAAGAAACCGACTTGTTTGATAACTGCTCTCGTTTTCTGTTTCTAGAATTTGAGAAATGTTTTTGAAAATGGGGTCAAATTTGAGAAATTACAAAAAGTAGCTTCTCTCTGTTTCATTTCTGTTGTATACATTATTATTATTATTATTATTTTTATCAAAACTCATCATTAGCCTACTGGTAGTCATGCACACTTATACTAAATATTTGGACTTTCATGTTTTCATATTTTAGAAATAGCACATTGTTCAAAACTTGTTAAAAGAGTTTTTTTTTTCGATCATCTCAGCCCAGATCGACTACCGAGATTTATATTAAAAAATTAACTTTTCATCTTATCTAATATATTTTGGGCTTTCTCGGAGAAATCTCGGGCATGATTAACACCGAGATTTTATATATTTTCCAAATCTTACATAATTTTTTTTGCAAAATTTGATCTGTTCCAAAATTTGAAAACAATATCCATTTAGAAAATCCACTTAGATAATTTGTAATTCCATTTGAAAATTTGAAATTTAGAAATTTGAAACAGAAATTGGGTGAAAGATTTGAAAATTTTTATATAAGATTTGGTAAAGAAAACAAATTTTGAAGAAAATTTGAAAAGATGTAAATTTTGGTATAACTTTTGGAAATATGACGTGATATTTAGAAAAAAAAATAGGAGAATCTCGTTGTTCATCCGTACGAGATTTTACTAAGAAAGTCCAAAACAATCGATATTAAATGAAAAAGTTAACTTTTTTTTTTTCAAATTTCAGTAGTCGATCTCGACCGAGATGGACCGAATCAAACTATTTTAACGAATTTTTTAAAAGGATGCTAGTTGTGAAAGAGAAACATTTCATATTATTTCTAACCATTGAATTTGAGAATTTATATACTTTTTTTTGACAGGGTAGAAAAAGAGTAATTAGGATGCTGGAAAACATGCTACATGAAAGGCAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGGTTTGAAATGATCTTTAAAAAAAAACAAAAACAAATTTAGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTGTTAATGATAGTTTGAAATTAGTTAATTAACAAGTGGAAAACTTAATTAATATAGTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGTAAGGAAAAATAATATGGTGTGTTAGATTTTAATAAATTACTTATTTAAATTATAAGTGGATTAAATTTTATATATATGGTTGCACAGGATACACAATTCCAGAAGGATGGGCTGTTATGGTTTGCCCTCCAGCCATTCACTTACATCCAAAAAATTACATTGATCCACTTGCCTTCAACCCATGGCGATGGGAGGTACCTTATTCAATTCAATATAATATATATATATATATATATATATTTTGACAATATGTAGAGAGGAAAGATCAAACATCTAATTTTGAACTTGATAGTACAAGTACTATGCTAATTGAACTATGCTCACACTTTGGCAATTTAATAATATTGTTGTTTTTAAATATGGAAAAATAAGTTGAACTATTTACAAATATAGAAAAAAATTTGCGATAGAAAGTTAAATTTTTTCTATATTTGTAAATAGTTTGATATTTTTTTTGTTTATAGTAATTTCCCTTAATAATATGTATCTTAATACCCCTCGCTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTCTTTTCTTTTTTTTTTTGTTAGAAATATTCACAAGTTTGTTATCAAACATTACATAGTTGAATTACAAAACTCCTAGATGATCAACAATAACATATTTGAAAATGATTTTTAAATGATCAAAATTATTTTTGAGGTGTCCAAAATTACTTTTCTTCTAAAATTTTGATGTTTGACACTGATGAGAAAATGATTTTGAAAACATCCAAAATTACTTAAAGTTGTGATTCACTATCAACTACTTGGAAAATAATTCTAAAGTGATTTTTGTTAGACGATTGTGAAGGGTATTTTTACTATTATTTCATATTTAAAAATTATGCTACTATTTCACTTTATTTTGTCCTAAAACAGTTTTTTTTAAATTTGTGTCCATTTTTATCTTTATTTCTCTCTAATTGTTTACCCTCTCCCTACTTTTCTGTAATGTTTAACTCTAATTTTATACTTTTTTTAAAAAAAAAAAATAATTTCTCACCTTTTCTCTCTCTAGTGGTTTTTAACTCTCTCTAGTTTTTTTATCTCGAAATTATTATATTATATTAATTAATAATAATAAGCAGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTAATTAATCTCTCTTATTAATTACCTTCTAAATTCCCATTTATATTAAAATAAACAATTAAAATTTATTTTTTATATTTTTTTATGCATATATTATCGTAGGGAGAGATGATTTAATGTAGTTTTTTCTTATATGGCATTTTTTACACTTAGGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

mRNA sequence

TGAGCAAAATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGATACACAATTCCAGAAGGATGGGCTGTTATGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

Coding sequence (CDS)

ATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGATACACAATTCCAGAAGGATGGGCTGTTATGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

Protein sequence

MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQQNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVKKKVVALQILTKTYKSSK

Homology

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BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 471.9 bits (1213), Expect = 8.9e-132
Identity = 238/501 (47.50%), Postives = 329/501 (65.67%), Query Frame = 0


Query: 12  SVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKT 71
           +++ W Y   + +  G+LP GS+G P+IGE+ QFFAPNP+ DLSPF+K+RI +YG IFKT
Sbjct: 54  ALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKERIKRYGSIFKT 113

Query: 72  RLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMII 131
            +VG+P+++SADPE+NY +FQQE +LFE WYP+TF +IFG   VGSLHGFM+KYLK +++
Sbjct: 114 SVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGFMYKYLKTLVL 173

Query: 132 NVFGIESLKNMIF-EVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSS 191
            ++G E+LK+++  E + A    L  WAS  +VELK+ I+ MIFDLTAK+LI YDP K S
Sbjct: 174 RLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKKLIGYDPSKPS 233

Query: 192 E-NLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------QNPRKQHIDYF 251
           + NLR+NF AFI GLISFPL+IPGT++H+C++                   P +   D+F
Sbjct: 234 QVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMAEPERPCEDFF 293

Query: 252 DYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSF 311
           D+V+QEL+++   LT+ IALDL+F+LLFAS+ETT++ALT+ +K L ++P V++ L     
Sbjct: 294 DHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENPKVVDAL----- 353

Query: 312 LDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFG 371
                                  + EHE I++ RK+ + G+TW EYKSMTFT +V     
Sbjct: 354 -----------------------REEHEAIIRNRKDPNSGVTWAEYKSMTFTSQV----- 413

Query: 372 KLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC-------------- 431
                   + E +RLANI PGIFR+AL+DVE KGYTIP GW +M C              
Sbjct: 414 --------IMEIVRLANIVPGIFRKALQDVEIKGYTIPAGWGIMVCPPAVHLNPEIYEDP 473

Query: 432 --------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGG 466
                          G +KHFMAFGGG+RFC+G D +K+ MA F+H+LVT Y+ + +KGG
Sbjct: 474 LAFNPWRWQGKPEITGGTKHFMAFGGGLRFCVGTDLSKVLMATFIHSLVTKYSWRTVKGG 513

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BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-80
Identity = 177/515 (34.37%), Postives = 267/515 (51.84%), Query Frame = 0


Query: 1   MWVV---MAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPF 60
           MW V   +A +F    I W+   + SK+ G LP G+MG P+IGE+ Q   P+ S D+ PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IKDRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFG--GQFV 120
           I+ ++ +YGPIFKT L G+P+++SAD E N  I  QE +  E WY +T  K FG   +++
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 GSLHGFMHKYLKNMIINVFGIESLKNMIFE-VEVAATTRLKKWASHNTVELKDEIANMIF 180
            +L G +HKY++++ +N FG E+L+      +E ++   L  W++  +VE+K+  A M+F
Sbjct: 121 KAL-GLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVF 180

Query: 181 DLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQQNPRKQH------ 240
             +  ++   D KK S N+   F   + G +S PL+ PGT++HKCL+     Q       
Sbjct: 181 RTSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVV 240

Query: 241 --------IDYFDYVLQELQKDGTP--LTQEIALDLIFLLLFASYETTSIALTLAIKFLI 300
                    D  D++ Q L+   +   +++E  + L+F + FAS+E+ S  LTL +K L 
Sbjct: 241 DDRLANVGPDVEDFLGQALKDKESEKFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 DHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEY 360
           +HP V+ EL                            +AEHE I K R +  G ITW EY
Sbjct: 301 EHPEVVKEL----------------------------EAEHEAIRKARADPDGPITWEEY 360

Query: 361 KSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC 420
           KSMTFT +V             +NETLRL ++ P + R+ +KD++ KGY IPEGW +M  
Sbjct: 361 KSMTFTLQV-------------INETLRLGSVTPALLRKTVKDLQVKGYIIPEGWTIMLV 420

Query: 421 DGA---------------------------SKHFMAFGGGMRFCIGADFAKMQMAVFLHN 467
             +                            K+FM FGGG+R C GA+++K+ +  FLH 
Sbjct: 421 TASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGGLRHCAGAEYSKVYLCTFLHI 473

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BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 259.2 bits (661), Expect = 9.1e-68
Identity = 164/508 (32.28%), Postives = 257/508 (50.59%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           +WV+  A+    +  WLY     K  GKLP GSMG PIIGE+  FF P+  +++SPF+K 
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGP+F+T + G   ++  +P++ + +F+QE + F   YPE F K FG + V   HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIINVFGIESL-KNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
            +HK++K + +   G E+L K MI E++      L+  A+  + + K+ + ++I      
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPG-TSFHKCL----------------QQ 240
           ++IS    ++   L +N +A        PL +    S +K                  ++
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
             R+   D+ D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++  
Sbjct: 249 ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENHK 308

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SM 360
            L EL                            + EH  IL+ R  K  G++W EY+  M
Sbjct: 309 ALAEL----------------------------KREHAAILQNRNGKGAGVSWEEYRHQM 368

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV------ 420
           TFT  V             +NETLR+AN+AP ++R+A+ DVE KGYTIP GW V      
Sbjct: 369 TFTNMV-------------INETLRMANMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPA 428

Query: 421 ----------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 463
                            R +G      SK FM FGGG+R C+GA+FA++Q+++F+H+LVT
Sbjct: 429 VHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQCVGAEFARLQISIFIHHLVT 473

[Close]

BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.7e-58
Identity = 147/456 (32.24%), Postives = 227/456 (49.78%), Query Frame = 0


Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           LP GS+G P+IGE+ Q      + +  PFI +R+ +YG +F T L G+P I SADPE N 
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLK-NMIFEVE 148
            + Q E +LFEC YP +   + G   +  + G +HK + ++ ++      +K +++ +++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 149 VAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISF 208
                 L  W+S   V L +E   + F+LT K+L+S+DP + SE+LR+ ++  I+G  S 
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 209 PLDIPGTSFHKCLQQNPRKQHIDYFDYVL---QELQKDGTP---------------LTQE 268
           PL +  T++ K +Q   R++  +    V+   +E +++G                  + E
Sbjct: 211 PLPLFSTTYRKAIQ--ARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAADDGFSDE 270

Query: 269 IALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLIS 328
             +D +  LL A YETTS  +TLA+KFL + P  L +L                      
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQL---------------------- 330

Query: 329 MQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLAN 388
                 + EHE+I +  K+ S  + W++YKSM FT            CV+  NETLR+AN
Sbjct: 331 ------KEEHEKI-RAMKSDSYSLEWSDYKSMPFT-----------QCVV--NETLRVAN 390

Query: 389 IAPGIFRRALKDVEFKGYTIPEGWAVMR----------------------------CDGA 438
           I  G+FRRA+ DVE KGY IP+GW V                                G 
Sbjct: 391 IIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGP 440

[Close]

BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 224.6 bits (571), Expect = 2.5e-57
Identity = 154/512 (30.08%), Postives = 238/512 (46.48%), Query Frame = 0


Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           LP G  G+P +GE+  +  P  +  L  F++  + KYG I+++ L G+P I+SAD  LN 
Sbjct: 39  LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNR 98

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIF-EVE 148
            I Q E +LFEC YP +   I G   +  L G MH+ ++++ +N      L+ ++  +VE
Sbjct: 99  FILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVE 158

Query: 149 VAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDP-KKSSENLRENFVAFIQGLIS 208
                 L  W  ++    +DE     F+L AK ++S DP ++ +E L++ +V F++G++S
Sbjct: 159 RHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVS 218

Query: 209 FPLDIPGTSFHKCLQ------------QNPRKQHIDYFDYVLQELQKDG----------- 268
            PL++PGT++HK LQ               RK  I   D   +E++ +            
Sbjct: 219 APLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVR 278

Query: 269 ---------------TPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELT 328
                          + L+ E  LDLI  LLFA +ET+S+A+ LAI FL   P  + EL 
Sbjct: 279 KQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEEL- 338

Query: 329 VHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKN-KSGGITWNEYKSMTFTFKV 388
                                      + EH  I + +K      + W++YK M FT   
Sbjct: 339 ---------------------------REEHLEIARAKKELGESELNWDDYKKMDFT--- 398

Query: 389 NEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM----------- 448
                    CVI  NETLRL N+   + R+ALKDV +KGY IP GW V+           
Sbjct: 399 --------QCVI--NETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNS 458

Query: 449 ---------------RCDGAS-----------KHFMAFGGGMRFCIGADFAKMQMAVFLH 463
                          + +GAS            ++M FGGG R C G++ AK++MAVF+H
Sbjct: 459 RYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIH 509

[Close]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL
Match: A0A0A0LCN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G845470 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 8.2e-197
Identity = 362/511 (70.84%), Postives = 395/511 (77.30%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MW ++ AI  FSV IW+Y LK  KY GKLPKGSMGFP +GES QFFAPNPSFDLSPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           RILKYGPIFKTRLVGKPLIISAD ELN+IIFQ+EE+LFECWYPETFRKIFG + VGSLHG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHN-TVELKDEIANMIFDLTAK 180
           FMHKYLKNMI NVFGIESLKNMI EVEV +TTRLKKWASHN  VELKDEIANMIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQ 240
           RLISYDP+K  EN+RENFVAFIQGLISFP+DIPGTS+HKCL                 QQ
Sbjct: 181 RLISYDPEKCGENMRENFVAFIQGLISFPIDIPGTSYHKCLQGRKRVMRMLENILKERQQ 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPR+Q +DYFD+V+QEL+KDGTPLTQ++ALDLIFLLLFAS+ETTSIALTLAIKFL DHPH
Sbjct: 241 NPREQQVDYFDFVIQELKKDGTPLTQQVALDLIFLLLFASHETTSIALTLAIKFLTDHPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VLN+LT                            AEHE ILK+RKNKS  I+WNEYKSMT
Sbjct: 301 VLNQLT----------------------------AEHEGILKKRKNKSDKISWNEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 420
           FTFK             F+NETLRLANIAPGIFRRAL+DVEFKGYTIPEGWAVM      
Sbjct: 361 FTFK-------------FMNETLRLANIAPGIFRRALRDVEFKGYTIPEGWAVMVCPPAI 420

Query: 421 ----------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 466
                                 RCDGASK+FMAFGGG+RFCIGADFAK+QMAVFLH+LVT
Sbjct: 421 HLDPKAFADPLAFNPWRWDEEQRCDGASKNFMAFGGGIRFCIGADFAKLQMAVFLHHLVT 470

[Close]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL
Match: A0A6J1J6X9 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.4e-191
Identity = 348/508 (68.50%), Postives = 385/508 (75.79%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MWV+MA IFG S+I  L+ LKYSKY  KLPKGSMGFPI+GE+  FFAPNPSFD+SPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+ KYGPIFKT LVGKPLIIS DP+LNY+IF+QEE LFECWYPETF+KIFG QF GSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKR 180
           FMHKYLKNMI+N FGIE LK+M+FEVE AA TRL++WAS   VELKDEIANMI DLTA +
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQN 240
           LISYDP+ S ENLRENFVAFIQGL+SFPLDIPGT++HKCL                 QQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHV 300
           PRKQ++D+FD+VLQELQKDGTPLT+EIALDLIFLLLFASYETTSIALTLAIKFL+DHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTF 360
           L++LT                             EHE ILK RKNK+ GITWNEYKSMTF
Sbjct: 301 LSQLT----------------------------EEHEGILKNRKNKASGITWNEYKSMTF 360

Query: 361 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------- 420
           TFK             FLNETLRLANIAPGIFRRAL+DVEFKG TIP GW VM       
Sbjct: 361 TFK-------------FLNETLRLANIAPGIFRRALRDVEFKGITIPAGWGVMVCPPAIH 420

Query: 421 ------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTL 467
                             RC+GASKHF+AFG G+RFCIGADFAK+QMAVFLHNLVT Y  
Sbjct: 421 LDPKHYVDPLAFNPWRWERCEGASKHFIAFGRGLRFCIGADFAKLQMAVFLHNLVTKYRF 467

[Close]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL
Match: A0A6J1F4V6 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442183 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 7.4e-190
Identity = 346/508 (68.11%), Postives = 385/508 (75.79%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MWV+MA IFG S+I  L+ L  SKY  KLPKGSMGFPI+GE+  FFAPNPSFD+SPFIKD
Sbjct: 1   MWVLMAIIFGMSIITCLHALIKSKYNAKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKD 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGPIFKT LVGKPLIIS DP+LNY+IF+QEE LFECWYPETF+KIFG QF GSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKR 180
           FMHKYLKNMI+N FGIE LK+M+ EVE AATTRL++WAS   VELKDEIAN+I DLTA +
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANVILDLTANK 180

Query: 181 LISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQN 240
           LISYDP+ S ENLRENFVAFIQGL+SFPLDIPGT++HKCL                 QQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHV 300
           PRKQ++D+FD+VLQELQKDGTPLT+EIALDLIFLLLFASYETTSIALTLAIKFL+DHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTF 360
           L +LT                             EHE ILK RK+K+ GITWNEYKSMTF
Sbjct: 301 LTQLT----------------------------EEHEGILKNRKDKASGITWNEYKSMTF 360

Query: 361 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------- 420
           TFK             FLNETLRLANIAPGIFRRAL+DVEFKG TIP GW VM       
Sbjct: 361 TFK-------------FLNETLRLANIAPGIFRRALRDVEFKGITIPAGWGVMVCPPAIH 420

Query: 421 ------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTL 467
                             RC+GAS+HF+AFG G+RFCIGADFAK+QMAVFLHNLVT Y  
Sbjct: 421 LDPKHYVDPLAFNPWRWERCEGASRHFIAFGRGLRFCIGADFAKLQMAVFLHNLVTKYRF 467

[Close]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL
Match: A0A5D3D7C7 (Cytochrome P450 87A3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00860 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 5.0e-162
Identity = 300/510 (58.82%), Postives = 364/510 (71.37%), Query Frame = 0


Query: 1   MWVVM-AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW+++  AI   S   W+Y  +  K  GKLP GSMG P++GE+ QFFAPN S D+ PFI+
Sbjct: 1   MWLLLIGAIVVLSFTHWVYSWRNPKCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIR 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            R+ +YGPIF+T LVG+PLI+S DP+LNY IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  KRMDRYGPIFRTSLVGRPLIVSTDPDLNYFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++++FG ESLK MI EVE  AT RLK+W+SHN VELKD+ A+MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLKKMIPEVEAVATRRLKQWSSHNPVELKDKTASMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD + SSENLR+NFVAFIQGLISFPL++PGT+++KCLQ                  
Sbjct: 181 KLISYDSESSSENLRDNFVAFIQGLISFPLNVPGTAYNKCLQGRKKAMRMLKNMLQERRA 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRKQ ID+FD+VL+EL+KDGT LT+EIALDL+F+LLFAS+ETTS+A+T AIKFL+++PH
Sbjct: 241 NPRKQRIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL ELT                            AEHE ILK R+    G+TW EYKSMT
Sbjct: 301 VLEELT----------------------------AEHEGILKRREISDSGLTWGEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FTF+             F+NET+RLANI PGIFR+ALKD++FKGYTIP GWAVM C    
Sbjct: 361 FTFQ-------------FINETVRLANIVPGIFRKALKDIQFKGYTIPSGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  +GASKHFMAFGGGMRFC+G DF K+QMAVFLH LVT 
Sbjct: 421 HLNPEKYVDPLAFNPWRWEKSELNGASKHFMAFGGGMRFCVGTDFTKVQMAVFLHCLVTK 469

[Close]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL
Match: A0A6J1DIX1 (cytochrome P450 87A3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020445 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 5.0e-162
Identity = 298/510 (58.43%), Postives = 365/510 (71.57%), Query Frame = 0


Query: 1   MWVVM-AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW+++  AI   S   WLY  +  K  GKLP GSMGFP++GE+ QFFAPN S D+ PFI+
Sbjct: 1   MWLLLVGAIVVLSFTHWLYSWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFIR 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            R+ +YGPIF+T LVG+ LI+S DP+LNY IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  RRMDRYGPIFRTSLVGRSLIVSTDPDLNYFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++++FG E+L+ M+ EVE  AT RL++W+SHN+VELKDE A+MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGSETLRKMVPEVEAVATRRLRQWSSHNSVELKDETASMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD + SSENLRENFVAFIQGLISFPLD+PGT++HKCLQ                  
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLDVPGTAYHKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRKQ ID+FD+VL+EL+KDGT LT+EIALDL+F+LLFAS+ETTS+A+T AIKFL+++PH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL ELT                            AEH+ ILK R+    G+TW EYKSMT
Sbjct: 301 VLEELT----------------------------AEHDGILKGRETADSGLTWAEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FTF+             F+NET+RLANI PGIFR+AL+D++FKGYTIP GWAVM C    
Sbjct: 361 FTFQ-------------FINETVRLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  +GASKHFMAFGGGMRFC+G DF K+QMAVFLH LVT 
Sbjct: 421 HLNPQKYTDPLEFNPWRWEKSELNGASKHFMAFGGGMRFCVGTDFTKVQMAVFLHCLVTK 469

[Close]

BLAST of Lsi03G012770 vs. NCBI nr
Match: XP_031737523.1 (cytochrome P450 87A3 isoform X2 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 9.9e-197
Identity = 362/509 (71.12%), Postives = 395/509 (77.60%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MW ++ AI  FSV IW+Y LK  KY GKLPKGSMGFP +GES QFFAPNPSFDLSPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           RILKYGPIFKTRLVGKPLIISAD ELN+IIFQ+EE+LFECWYPETFRKIFG + VGSLHG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHN-TVELKDEIANMIFDLTAK 180
           FMHKYLKNMI NVFGIESLKNMI EVEV +TTRLKKWASHN  VELKDEIANMIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQ 240
           RLISYDP+K  EN+RENFVAFIQGLISFP+DIPGTS+HKCL                 QQ
Sbjct: 181 RLISYDPEKCGENMRENFVAFIQGLISFPIDIPGTSYHKCLQGRKRVMRMLENILKERQQ 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPR+Q +DYFD+V+QEL+KDGTPLTQ++ALDLIFLLLFAS+ETTSIALTLAIKFL DHPH
Sbjct: 241 NPREQQVDYFDFVIQELKKDGTPLTQQVALDLIFLLLFASHETTSIALTLAIKFLTDHPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VLN+LT                            AEHE ILK+RKNKS  I+WNEYKSMT
Sbjct: 301 VLNQLT----------------------------AEHEGILKKRKNKSDKISWNEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 420
           FTFK             F+NETLRLANIAPGIFRRAL+DVEFKGYTIPEGWAVM      
Sbjct: 361 FTFK-------------FMNETLRLANIAPGIFRRALRDVEFKGYTIPEGWAVMVCPPAI 420

Query: 421 --------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNY 466
                               RCDGASK+FMAFGGG+RFCIGADFAK+QMAVFLH+LVTNY
Sbjct: 421 HLDPKAFADPLAFNPWRWDERCDGASKNFMAFGGGIRFCIGADFAKLQMAVFLHHLVTNY 468

[Close]

BLAST of Lsi03G012770 vs. NCBI nr
Match: XP_004141154.1 (cytochrome P450 87A3 isoform X1 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 1.7e-196
Identity = 362/511 (70.84%), Postives = 395/511 (77.30%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MW ++ AI  FSV IW+Y LK  KY GKLPKGSMGFP +GES QFFAPNPSFDLSPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           RILKYGPIFKTRLVGKPLIISAD ELN+IIFQ+EE+LFECWYPETFRKIFG + VGSLHG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHN-TVELKDEIANMIFDLTAK 180
           FMHKYLKNMI NVFGIESLKNMI EVEV +TTRLKKWASHN  VELKDEIANMIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQ 240
           RLISYDP+K  EN+RENFVAFIQGLISFP+DIPGTS+HKCL                 QQ
Sbjct: 181 RLISYDPEKCGENMRENFVAFIQGLISFPIDIPGTSYHKCLQGRKRVMRMLENILKERQQ 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPR+Q +DYFD+V+QEL+KDGTPLTQ++ALDLIFLLLFAS+ETTSIALTLAIKFL DHPH
Sbjct: 241 NPREQQVDYFDFVIQELKKDGTPLTQQVALDLIFLLLFASHETTSIALTLAIKFLTDHPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VLN+LT                            AEHE ILK+RKNKS  I+WNEYKSMT
Sbjct: 301 VLNQLT----------------------------AEHEGILKKRKNKSDKISWNEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 420
           FTFK             F+NETLRLANIAPGIFRRAL+DVEFKGYTIPEGWAVM      
Sbjct: 361 FTFK-------------FMNETLRLANIAPGIFRRALRDVEFKGYTIPEGWAVMVCPPAI 420

Query: 421 ----------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 466
                                 RCDGASK+FMAFGGG+RFCIGADFAK+QMAVFLH+LVT
Sbjct: 421 HLDPKAFADPLAFNPWRWDEEQRCDGASKNFMAFGGGIRFCIGADFAKLQMAVFLHHLVT 470

[Close]

BLAST of Lsi03G012770 vs. NCBI nr
Match: XP_022983294.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 679.1 bits (1751), Expect = 2.8e-191
Identity = 348/508 (68.50%), Postives = 385/508 (75.79%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MWV+MA IFG S+I  L+ LKYSKY  KLPKGSMGFPI+GE+  FFAPNPSFD+SPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+ KYGPIFKT LVGKPLIIS DP+LNY+IF+QEE LFECWYPETF+KIFG QF GSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKR 180
           FMHKYLKNMI+N FGIE LK+M+FEVE AA TRL++WAS   VELKDEIANMI DLTA +
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQN 240
           LISYDP+ S ENLRENFVAFIQGL+SFPLDIPGT++HKCL                 QQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHV 300
           PRKQ++D+FD+VLQELQKDGTPLT+EIALDLIFLLLFASYETTSIALTLAIKFL+DHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTF 360
           L++LT                             EHE ILK RKNK+ GITWNEYKSMTF
Sbjct: 301 LSQLT----------------------------EEHEGILKNRKNKASGITWNEYKSMTF 360

Query: 361 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------- 420
           TFK             FLNETLRLANIAPGIFRRAL+DVEFKG TIP GW VM       
Sbjct: 361 TFK-------------FLNETLRLANIAPGIFRRALRDVEFKGITIPAGWGVMVCPPAIH 420

Query: 421 ------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTL 467
                             RC+GASKHF+AFG G+RFCIGADFAK+QMAVFLHNLVT Y  
Sbjct: 421 LDPKHYVDPLAFNPWRWERCEGASKHFIAFGRGLRFCIGADFAKLQMAVFLHNLVTKYRF 467

[Close]

BLAST of Lsi03G012770 vs. NCBI nr
Match: XP_022935244.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 673.3 bits (1736), Expect = 1.5e-189
Identity = 346/508 (68.11%), Postives = 385/508 (75.79%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MWV+MA IFG S+I  L+ L  SKY  KLPKGSMGFPI+GE+  FFAPNPSFD+SPFIKD
Sbjct: 1   MWVLMAIIFGMSIITCLHALIKSKYNAKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKD 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGPIFKT LVGKPLIIS DP+LNY+IF+QEE LFECWYPETF+KIFG QF GSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKR 180
           FMHKYLKNMI+N FGIE LK+M+ EVE AATTRL++WAS   VELKDEIAN+I DLTA +
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANVILDLTANK 180

Query: 181 LISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQN 240
           LISYDP+ S ENLRENFVAFIQGL+SFPLDIPGT++HKCL                 QQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHV 300
           PRKQ++D+FD+VLQELQKDGTPLT+EIALDLIFLLLFASYETTSIALTLAIKFL+DHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTF 360
           L +LT                             EHE ILK RK+K+ GITWNEYKSMTF
Sbjct: 301 LTQLT----------------------------EEHEGILKNRKDKASGITWNEYKSMTF 360

Query: 361 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------- 420
           TFK             FLNETLRLANIAPGIFRRAL+DVEFKG TIP GW VM       
Sbjct: 361 TFK-------------FLNETLRLANIAPGIFRRALRDVEFKGITIPAGWGVMVCPPAIH 420

Query: 421 ------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTL 467
                             RC+GAS+HF+AFG G+RFCIGADFAK+QMAVFLHNLVT Y  
Sbjct: 421 LDPKHYVDPLAFNPWRWERCEGASRHFIAFGRGLRFCIGADFAKLQMAVFLHNLVTKYRF 467

[Close]

BLAST of Lsi03G012770 vs. NCBI nr
Match: KAG6581642.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630.2 bits (1624), Expect = 1.5e-176
Identity = 321/475 (67.58%), Postives = 361/475 (76.00%), Query Frame = 0


Query: 34  MGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNYIIFQQ 93
           MGFPI+GE+  FF+PNPSFD+SPFIK R+LKYGPIFKT LVGKPLIIS DP+LNY+IF+Q
Sbjct: 1   MGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 60

Query: 94  EEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTR 153
           EE LFECWYPETF+KIFG QF GSLHGFMHKYLKNMI+N FGIE LK+++ EVE AATTR
Sbjct: 61  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSIVSEVEAAATTR 120

Query: 154 LKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPG 213
           L++WAS   VELKDEIANMI DLTA +LISYDP+ S ENLRENFVAFIQGL+SFPLDIPG
Sbjct: 121 LRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 180

Query: 214 TSFHKCL-----------------QQNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIF 273
           T++HKCL                 QQNPRKQ++D+FD+VLQEL+KDGTPLT+EIALDLIF
Sbjct: 181 TAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQELRKDGTPLTKEIALDLIF 240

Query: 274 LLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQ 333
           LLLFASYETTSIALTLAIKFL+DHPHVL++LT                            
Sbjct: 241 LLLFASYETTSIALTLAIKFLLDHPHVLSQLT---------------------------- 300

Query: 334 AEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFR 393
            EHE ILK RK+K+ GITWNEYKSMTFTFK             FLNETLRLANIAPGIFR
Sbjct: 301 EEHEGILKNRKDKASGITWNEYKSMTFTFK-------------FLNETLRLANIAPGIFR 360

Query: 394 RALKDVEFKGYTIPEGWAVM-------------------------RCDGASKHFMAFGGG 453
           RAL+DVEFKG TIP GW VM                         RC+GASKHF+AFG G
Sbjct: 361 RALRDVEFKGITIPAGWGVMVCPPAIHLDPKHYVDPLAFNPWRWERCEGASKHFIAFGRG 420

Query: 454 MRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVKKK 467
           +RFC+GADFAK+QMAVFLH+LVT Y  KAI GGNIVRTPGVQFPDGFHVEIV+K+
Sbjct: 421 LRFCVGADFAKLQMAVFLHSLVTKYRFKAIGGGNIVRTPGVQFPDGFHVEIVEKE 434

[Close]

BLAST of Lsi03G012770 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 531.9 bits (1369), Expect = 5.1e-151
Identity = 272/512 (53.12%), Postives = 345/512 (67.38%), Query Frame = 0


Query: 1   MW--VVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFI 60
           MW  ++  ++   S+  W+Y  +  K  GKLP GSMGFP++GES QFF PN + D+ PFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  KDRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSL 120
           K+R+ KYGPIFKT LVG+P+I+S D +L+Y +F QE + F+ WYP+TF  IFG + VGSL
Sbjct: 61  KERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSL 120

Query: 121 HGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTA 180
           HGFM+KYLKNM++ +FG + LK M+ +VE+ A  RL+ W++ ++VELKD  A+MIFDLTA
Sbjct: 121 HGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTA 180

Query: 181 KRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ----------------- 240
           K+LIS+DP KSSENLR NFVAFIQGLISFP DIPGT++HKCLQ                 
Sbjct: 181 KKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR 240

Query: 241 QNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHP 300
           +NPRK   D+FDYV++E+QK+GT LT+EIALDL+F+LLFAS+ETTS+ALTLAIKFL D P
Sbjct: 241 ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDP 300

Query: 301 HVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSM 360
            VL  LT                             EHE IL+ R++   G+TW EYKSM
Sbjct: 301 EVLKRLT----------------------------EEHETILRNREDADSGLTWEEYKSM 360

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC--- 420
           T+TF+             F+NET RLANI P IFR+AL+D++FK YTIP GWAVM C   
Sbjct: 361 TYTFQ-------------FINETARLANIVPAIFRKALRDIKFKDYTIPAGWAVMVCPPA 420

Query: 421 ------------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 467
                                     ASKHFMAFGGGMRFC+G DF K+QMA FLH+LVT
Sbjct: 421 VHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGTDFTKLQMAAFLHSLVT 471

[Close]

BLAST of Lsi03G012770 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 524.6 bits (1350), Expect = 8.2e-149
Identity = 271/518 (52.32%), Postives = 345/518 (66.60%), Query Frame = 0


Query: 1   MW--VVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFI 60
           MW  ++  ++   S+  W+Y  +  K  GKLP GSMGFP++GES QFF PN + D+ PFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  KDRI------LKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGG 120
           K+R+       +YGPIFKT LVG+P+I+S D +L+Y +F QE + F+ WYP+TF  IFG 
Sbjct: 61  KERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGK 120

Query: 121 QFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANM 180
           + VGSLHGFM+KYLKNM++ +FG + LK M+ +VE+ A  RL+ W++ ++VELKD  A+M
Sbjct: 121 KNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASM 180

Query: 181 IFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ----------- 240
           IFDLTAK+LIS+DP KSSENLR NFVAFIQGLISFP DIPGT++HKCLQ           
Sbjct: 181 IFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRN 240

Query: 241 ------QNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIK 300
                 +NPRK   D+FDYV++E+QK+GT LT+EIALDL+F+LLFAS+ETTS+ALTLAIK
Sbjct: 241 MLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIK 300

Query: 301 FLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITW 360
           FL D P VL  LT                             EHE IL+ R++   G+TW
Sbjct: 301 FLSDDPEVLKRLT----------------------------EEHETILRNREDADSGLTW 360

Query: 361 NEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV 420
            EYKSMT+TF+             F+NET RLANI P IFR+AL+D++FK YTIP GWAV
Sbjct: 361 EEYKSMTYTFQ-------------FINETARLANIVPAIFRKALRDIKFKDYTIPAGWAV 420

Query: 421 MRC---------------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVF 467
           M C                             ASKHFMAFGGGMRFC+G DF K+QMA F
Sbjct: 421 MVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGTDFTKLQMAAF 477

[Close]

BLAST of Lsi03G012770 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 263.8 bits (673), Expect = 2.6e-70
Identity = 168/506 (33.20%), Postives = 254/506 (50.20%), Query Frame = 0


Query: 3   VVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRI 62
           ++M A+    +  WLY     K  GKLP GSMGFPIIGE+  FF P     +  F+K R+
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 63  LKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFM 122
           ++YGP+F+T + G   ++S DP++ + IF+QE   FE  YP+ F K+FG   +     F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 123 HKYLKNMIINVFGIESLK-NMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRL 182
           HKYL+ + + + G E LK  M+  ++ A    ++  AS  +  ++ E+ N++      +L
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 183 ISYDPKKSSENLRENFVAF-----------------IQGLISFPLDIPGTSFHKCLQQNP 242
           IS    ++   L +N  AF                  + L S    I        +++  
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRKET 248

Query: 243 RKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVL 302
           R++  D+ + +L+EL+KDG+   Q  A++LIFLL FA  E TS    LA+KF+   P VL
Sbjct: 249 REKQEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKVL 308

Query: 303 NELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SMTF 362
            EL                            + EH+ I+  RK+K  G++W EY+ +MTF
Sbjct: 309 AEL----------------------------KREHKAIVDNRKDKEAGVSWEEYRHNMTF 368

Query: 363 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV-------- 422
           T  V+             NE LRLAN  P +FR+A++DVE KGYTIP GW V        
Sbjct: 369 TNMVS-------------NEVLRLANTTPLLFRKAVQDVEIKGYTIPAGWIVAVAPSAVH 428

Query: 423 --------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNY 463
                          R +G      SK FMAFG G+R C+GA+F+++QMA+FLH+LV  Y
Sbjct: 429 FDPAIYENPFEFNPWRWEGKEMIWGSKTFMAFGYGVRLCVGAEFSRLQMAIFLHHLVAYY 473

[Close]

BLAST of Lsi03G012770 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-69
Identity = 164/508 (32.28%), Postives = 257/508 (50.59%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           +WV+  A+    +  WLY     K  GKLP GSMG PIIGE+  FF P+  +++SPF+K 
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGP+F+T + G   ++  +P++ + +F+QE + F   YPE F K FG + V   HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIINVFGIESL-KNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
            +HK++K + +   G E+L K MI E++      L+  A+  + + K+ + ++I      
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPG-TSFHKCL----------------QQ 240
           ++IS    ++   L +N +A        PL +    S +K                  ++
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
             R+   D+ D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++  
Sbjct: 249 ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENHK 308

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SM 360
            L EL                            + EH  IL+ R  K  G++W EY+  M
Sbjct: 309 ALAEL----------------------------KREHAAILQNRNGKGAGVSWEEYRHQM 368

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV------ 420
           TFT  V             +NETLR+AN+AP ++R+A+ DVE KGYTIP GW V      
Sbjct: 369 TFTNMV-------------INETLRMANMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPA 428

Query: 421 ----------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 463
                            R +G      SK FM FGGG+R C+GA+FA++Q+++F+H+LVT
Sbjct: 429 VHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQCVGAEFARLQISIFIHHLVT 473

[Close]

BLAST of Lsi03G012770 vs. TAIR 10
Match: AT5G48000.3 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-69
Identity = 164/508 (32.28%), Postives = 257/508 (50.59%), Query Frame = 0


Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           +WV+  A+    +  WLY     K  GKLP GSMG PIIGE+  FF P+  +++SPF+K 
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGP+F+T + G   ++  +P++ + +F+QE + F   YPE F K FG + V   HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIINVFGIESL-KNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
            +HK++K + +   G E+L K MI E++      L+  A+  + + K+ + ++I      
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPG-TSFHKCL----------------QQ 240
           ++IS    ++   L +N +A        PL +    S +K                  ++
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
             R+   D+ D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++  
Sbjct: 249 ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENHK 308

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SM 360
            L EL                            + EH  IL+ R  K  G++W EY+  M
Sbjct: 309 ALAEL----------------------------KREHAAILQNRNGKGAGVSWEEYRHQM 368

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV------ 420
           TFT  V             +NETLR+AN+AP ++R+A+ DVE KGYTIP GW V      
Sbjct: 369 TFTNMV-------------INETLRMANMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPA 428

Query: 421 ----------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 463
                            R +G      SK FM FGGG+R C+GA+FA++Q+++F+H+LVT
Sbjct: 429 VHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQCVGAEFARLQISIFIHHLVT 473

The following BLAST results are available for this feature:

BLAST of Lsi03G012770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XU38	8.9e-132	47.50	Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV...	[more]
K7NBR2	2.1e-80	34.37	Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=...	[more]
Q8L7D5	9.1e-68	32.28	Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3	[more]
Q42569	1.7e-58	32.24	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
O64989	2.5e-57	30.08	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]

BLAST of Lsi03G012770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCN3	8.2e-197	70.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G845470 PE=3 SV=1	[more]
A0A6J1J6X9	1.4e-191	68.50	cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481917...	[more]
A0A6J1F4V6	7.4e-190	68.11	cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114421...	[more]
A0A5D3D7C7	5.0e-162	58.82	Cytochrome P450 87A3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1DIX1	5.0e-162	58.43	cytochrome P450 87A3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020445 P...	[more]

BLAST of Lsi03G012770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737523.1	9.9e-197	71.12	cytochrome P450 87A3 isoform X2 [Cucumis sativus]	[more]
XP_004141154.1	1.7e-196	70.84	cytochrome P450 87A3 isoform X1 [Cucumis sativus]	[more]
XP_022983294.1	2.8e-191	68.50	cytochrome P450 87A3-like isoform X1 [Cucurbita maxima]	[more]
XP_022935244.1	1.5e-189	68.11	cytochrome P450 87A3-like isoform X1 [Cucurbita moschata]	[more]
KAG6581642.1	1.5e-176	67.58	Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi03G012770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12740.1	5.1e-151	53.13	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	8.2e-149	52.32	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G78490.1	2.6e-70	33.20	cytochrome P450, family 708, subfamily A, polypeptide 3	[more]
AT5G48000.2	6.4e-69	32.28	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]
AT5G48000.3	6.4e-69	32.28	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 415..438 score: 27.38 coord: 405..415 score: 49.14 coord: 62..81 score: 26.03
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 261..278 score: 33.16 coord: 359..370 score: 35.31 coord: 415..426 score: 35.18 coord: 406..415 score: 49.71
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 401..447 e-value: 2.5E-10 score: 39.7 coord: 361..396 e-value: 1.7E-6 score: 27.1 coord: 223..287 e-value: 4.9E-7 score: 28.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 22..397 e-value: 1.4E-63 score: 217.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 398..456 e-value: 2.4E-10 score: 41.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..452
None	No IPR available	PANTHER	PTHR24286:SF11	CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2	coord: 402..460 coord: 11..398
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 402..460 coord: 11..398
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 408..417

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G012770.1	Lsi03G012770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen