Lsi03G012320 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G012320 vs. ExPASy TrEMBL
Match: A0A5D3CGK3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00070 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.5e-143
Identity = 259/306 (84.64%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKASI+FREDQN  F AKIPL FFG PFRSAI L ++DNLSFTF TFFRSGPSFNLSYRP
Sbjct: 1   MKASIIFREDQNLTFTAKIPLCFFGLPFRSAIYLPDSDNLSFTFATFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           NH+LNPFSLA+KAGIGLFGSPIDSPMTF AEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ
Sbjct: 61  NHALNPFSLALKAGIGLFGSPIDSPMTFAAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S IA FN P RN ISQVDDD +ALSRGKS+DG ET DLGLG+PVLS QRIDCSEV NRVD
Sbjct: 121 SGIAIFNFPYRNLISQVDDDASALSRGKSIDGPETPDLGLGNPVLSSQRIDCSEVFNRVD 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKIER 240
           DV ST+EINARSTFKV DS AVKFRWSMRFPTSM K+ FTAK PLSKMPYLALGKIKIER
Sbjct: 181 DVFSTMEINARSTFKVGDSTAVKFRWSMRFPTSMNKDGFTAKAPLSKMPYLALGKIKIER 240

Query: 241 AAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPPVE 300
           AAAS+GERE+NE GGAGEFS +KKHL+DLW ESRWLKKN+EQLQSEI +QKA P TPPVE
Sbjct: 241 AAASEGERESNEAGGAGEFSGMKKHLEDLWKESRWLKKNVEQLQSEIGEQKAGPTTPPVE 300

Query: 301 TRKKKG 307
           TRKKKG
Sbjct: 301 TRKKKG 306

BLAST of Lsi03G012320 vs. ExPASy TrEMBL
Match: A0A1S3CFN0 (uncharacterized protein LOC103500380 OS=Cucumis melo OX=3656 GN=LOC103500380 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.5e-143
Identity = 259/306 (84.64%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKASI+FREDQN  F AKIPL FFG PFRSAI L ++DNLSFTF TFFRSGPSFNLSYRP
Sbjct: 1   MKASIIFREDQNLTFTAKIPLCFFGLPFRSAIYLPDSDNLSFTFATFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           NH+LNPFSLA+KAGIGLFGSPIDSPMTF AEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ
Sbjct: 61  NHALNPFSLALKAGIGLFGSPIDSPMTFAAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S IA FN P RN ISQVDDD +ALSRGKS+DG ET DLGLG+PVLS QRIDCSEV NRVD
Sbjct: 121 SGIAIFNFPYRNLISQVDDDASALSRGKSIDGPETPDLGLGNPVLSSQRIDCSEVFNRVD 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKIER 240
           DV ST+EINARSTFKV DS AVKFRWSMRFPTSM K+ FTAK PLSKMPYLALGKIKIER
Sbjct: 181 DVFSTMEINARSTFKVGDSTAVKFRWSMRFPTSMNKDGFTAKAPLSKMPYLALGKIKIER 240

Query: 241 AAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPPVE 300
           AAAS+GERE+NE GGAGEFS +KKHL+DLW ESRWLKKN+EQLQSEI +QKA P TPPVE
Sbjct: 241 AAASEGERESNEAGGAGEFSGMKKHLEDLWKESRWLKKNVEQLQSEIGEQKAGPTTPPVE 300

Query: 301 TRKKKG 307
           TRKKKG
Sbjct: 301 TRKKKG 306

BLAST of Lsi03G012320 vs. ExPASy TrEMBL
Match: A0A0A0LD41 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G702600 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 6.9e-141
Identity = 255/306 (83.33%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKAS++FREDQN  FRAKIPLNFFG PFRS++ L ++DNLSFTF TFFRSGPSFNLSYRP
Sbjct: 1   MKASLIFREDQNLSFRAKIPLNFFGLPFRSSVHLPDSDNLSFTFATFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           N +LNPFSLA+KAGIGLFGSPIDSPMTF AEFNLP NQPPRFFLHFRPQLGDFTLRRSVQ
Sbjct: 61  NQALNPFSLAIKAGIGLFGSPIDSPMTFAAEFNLPANQPPRFFLHFRPQLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S+I NF LP RN ISQVDDDV+A +RGKSV G ET DLGLG+PVLS QRID SE LNRVD
Sbjct: 121 SNITNFKLPYRNLISQVDDDVSAFTRGKSVVGPETPDLGLGNPVLSSQRIDFSEALNRVD 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKIER 240
           DVLST+EINARSTFKV DS AVKFRWSM FPT MKK+EFTAK PLSKMPYLALGKIKIER
Sbjct: 181 DVLSTMEINARSTFKVGDSTAVKFRWSMTFPTCMKKDEFTAKAPLSKMPYLALGKIKIER 240

Query: 241 AAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPPVE 300
           AAAS+ ERE+N+  GAGEFS LKKHL+DLW ESRWLKKNIEQLQSEI +QKAAP+TPPVE
Sbjct: 241 AAASESERESNKAAGAGEFSGLKKHLEDLWKESRWLKKNIEQLQSEIGEQKAAPSTPPVE 300

Query: 301 TRKKKG 307
           TRKKKG
Sbjct: 301 TRKKKG 306

BLAST of Lsi03G012320 vs. ExPASy TrEMBL
Match: A0A6J1F7Z3 (uncharacterized protein LOC111441692 OS=Cucurbita moschata OX=3662 GN=LOC111441692 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.2e-123
Identity = 239/312 (76.60%), Postives = 264/312 (84.62%), Query Frame = 0

Query: 1   MKASIVFREDQ-NPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYR 60
           MKASI+FREDQ NPIFRAKIPLNFFGFPFRSAI+LAE DNLSFTF +FFRSGP+FN SYR
Sbjct: 1   MKASIIFREDQRNPIFRAKIPLNFFGFPFRSAIQLAEPDNLSFTFTSFFRSGPAFNFSYR 60

Query: 61  PNHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSV 120
           PN SL PF+LAVKAGIGL GS IDSPM FTAEFNLPGN+PPRFFLHFRP+LGDFTLRRSV
Sbjct: 61  PNDSLAPFTLAVKAGIGLHGSSIDSPMNFTAEFNLPGNKPPRFFLHFRPRLGDFTLRRSV 120

Query: 121 QSHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETS-----DLGLGHPVLSIQRIDCSE 180
           QSH A+FNLP       +DDD+AA+S GKSVD GE+S     DLGLG+ +LS QRI CS 
Sbjct: 121 QSHTASFNLP-------LDDDLAAMSSGKSVDSGESSGEVHTDLGLGNTMLSGQRIHCSG 180

Query: 181 VLNRVDDVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAK-LPLSKMPYLAL 240
           + NR  D+LST EINARSTFKV++SAAVKF+W MRFP SMKKEEFTAK + LSK+PYLAL
Sbjct: 181 IFNRFHDLLSTGEINARSTFKVKNSAAVKFQWCMRFPMSMKKEEFTAKVMLLSKLPYLAL 240

Query: 241 GKIKIERAAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAA 300
            KIKIE AAASD ERE+NE   AGE S LKKHLDDL TESRW+KKNIEQL+SEI DQ AA
Sbjct: 241 RKIKIELAAASDSERESNEAVSAGEISVLKKHLDDLRTESRWMKKNIEQLRSEIGDQVAA 300

Query: 301 PATPPVETRKKK 306
           PA PPVE+RKKK
Sbjct: 301 PANPPVESRKKK 305

BLAST of Lsi03G012320 vs. ExPASy TrEMBL
Match: A0A6J1DED2 (uncharacterized protein LOC111020281 OS=Momordica charantia OX=3673 GN=LOC111020281 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 9.4e-122
Identity = 229/313 (73.16%), Postives = 266/313 (84.98%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKASI+FR+DQNPI RAKIPLNFFGFP RSAIE+A+  +LSFTF+TFFRSGPSFNLSYRP
Sbjct: 1   MKASIIFRDDQNPILRAKIPLNFFGFPLRSAIEVADVHDLSFTFSTFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           N S+NPF+LA+KAGIGLFGSPID+PM FTAEFNLP N+PPRFFLHFRP+LGDFTLRRSVQ
Sbjct: 61  NDSINPFTLALKAGIGLFGSPIDAPMAFTAEFNLPRNEPPRFFLHFRPRLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSV-DGGET-----SDLGLGHPVLSIQRIDCSE 180
           SHI N NLP RN+IS++DDDVAALS+GKSV DG ET     SDL LG+       IDCS+
Sbjct: 121 SHIPNLNLPYRNAISRMDDDVAALSKGKSVDDGAETSGEADSDLTLGN------GIDCSQ 180

Query: 181 VLNRVDDVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALG 240
           V  R+DDVLS+VEI A S FKVRD AAVKFRW+MRFP S++ E+FTA+  LSKMPYL LG
Sbjct: 181 VXTRIDDVLSSVEICAXSRFKVRDRAAVKFRWTMRFPVSVRTEDFTARTLLSKMPYLTLG 240

Query: 241 KIKIERAAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAP 300
           KIKIE      GE+E++E  GAGE+SAL+K LDDLW+ESRWLK N+EQL+SEI +QKAAP
Sbjct: 241 KIKIEGV----GEQESDEAAGAGEYSALRKQLDDLWSESRWLKMNVEQLRSEIGEQKAAP 300

Query: 301 ATPPVETRKKKGA 308
           ATPPVE+RK++GA
Sbjct: 301 ATPPVESRKRRGA 303

BLAST of Lsi03G012320 vs. NCBI nr
Match: XP_038904343.1 (uncharacterized protein LOC120090696 [Benincasa hispida])

HSP 1 Score: 546.6 bits (1407), Expect = 1.4e-151
Identity = 278/307 (90.55%), Postives = 288/307 (93.81%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKASI+FR+DQNPIFRAKIPLNFFGFPFRSAI LAEADNLSFTF TFFRSGPSFNLSY P
Sbjct: 1   MKASIIFRDDQNPIFRAKIPLNFFGFPFRSAIHLAEADNLSFTFATFFRSGPSFNLSYHP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           NHSLNPFSLAVKAGIGLFGSPIDSPMT TAEFNLPGNQPPRFFLHF+PQLGDFTLRRS+Q
Sbjct: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTVTAEFNLPGNQPPRFFLHFKPQLGDFTLRRSLQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSD--LGLGHPVLSIQRIDCSEVLNR 180
           SHIANFNLP RNSISQVD  VAALSRGKSVDGGETSD  LGLG+ VLS QRIDCSEV NR
Sbjct: 121 SHIANFNLPYRNSISQVDYHVAALSRGKSVDGGETSDLRLGLGNAVLSSQRIDCSEVWNR 180

Query: 181 VDDVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKI 240
           VDDVLSTVEINARSTFK+RDSAAVKFRWSMRFPTSMKKE+F AK+P SKMPYLALGKIKI
Sbjct: 181 VDDVLSTVEINARSTFKLRDSAAVKFRWSMRFPTSMKKEDFLAKIPPSKMPYLALGKIKI 240

Query: 241 ERAAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPP 300
           ERA ASD ERE+NE GGAGEF ALKKHLDDLWTESRWLKKNIE+LQSEI DQKAAPATPP
Sbjct: 241 ERAMASDSERESNEAGGAGEFLALKKHLDDLWTESRWLKKNIEELQSEIGDQKAAPATPP 300

Query: 301 VETRKKK 306
           VETRKKK
Sbjct: 301 VETRKKK 307

BLAST of Lsi03G012320 vs. NCBI nr
Match: XP_008461888.1 (PREDICTED: uncharacterized protein LOC103500380 [Cucumis melo] >KAA0048300.1 uncharacterized protein E6C27_scaffold264G00090 [Cucumis melo var. makuwa] >TYK10645.1 uncharacterized protein E5676_scaffold25G00070 [Cucumis melo var. makuwa])

HSP 1 Score: 518.1 bits (1333), Expect = 5.3e-143
Identity = 259/306 (84.64%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKASI+FREDQN  F AKIPL FFG PFRSAI L ++DNLSFTF TFFRSGPSFNLSYRP
Sbjct: 1   MKASIIFREDQNLTFTAKIPLCFFGLPFRSAIYLPDSDNLSFTFATFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           NH+LNPFSLA+KAGIGLFGSPIDSPMTF AEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ
Sbjct: 61  NHALNPFSLALKAGIGLFGSPIDSPMTFAAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S IA FN P RN ISQVDDD +ALSRGKS+DG ET DLGLG+PVLS QRIDCSEV NRVD
Sbjct: 121 SGIAIFNFPYRNLISQVDDDASALSRGKSIDGPETPDLGLGNPVLSSQRIDCSEVFNRVD 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKIER 240
           DV ST+EINARSTFKV DS AVKFRWSMRFPTSM K+ FTAK PLSKMPYLALGKIKIER
Sbjct: 181 DVFSTMEINARSTFKVGDSTAVKFRWSMRFPTSMNKDGFTAKAPLSKMPYLALGKIKIER 240

Query: 241 AAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPPVE 300
           AAAS+GERE+NE GGAGEFS +KKHL+DLW ESRWLKKN+EQLQSEI +QKA P TPPVE
Sbjct: 241 AAASEGERESNEAGGAGEFSGMKKHLEDLWKESRWLKKNVEQLQSEIGEQKAGPTTPPVE 300

Query: 301 TRKKKG 307
           TRKKKG
Sbjct: 301 TRKKKG 306

BLAST of Lsi03G012320 vs. NCBI nr
Match: XP_011652835.1 (uncharacterized protein LOC105435123 [Cucumis sativus] >KGN58622.1 hypothetical protein Csa_002013 [Cucumis sativus])

HSP 1 Score: 510.0 bits (1312), Expect = 1.4e-140
Identity = 255/306 (83.33%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKAS++FREDQN  FRAKIPLNFFG PFRS++ L ++DNLSFTF TFFRSGPSFNLSYRP
Sbjct: 1   MKASLIFREDQNLSFRAKIPLNFFGLPFRSSVHLPDSDNLSFTFATFFRSGPSFNLSYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           N +LNPFSLA+KAGIGLFGSPIDSPMTF AEFNLP NQPPRFFLHFRPQLGDFTLRRSVQ
Sbjct: 61  NQALNPFSLAIKAGIGLFGSPIDSPMTFAAEFNLPANQPPRFFLHFRPQLGDFTLRRSVQ 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S+I NF LP RN ISQVDDDV+A +RGKSV G ET DLGLG+PVLS QRID SE LNRVD
Sbjct: 121 SNITNFKLPYRNLISQVDDDVSAFTRGKSVVGPETPDLGLGNPVLSSQRIDFSEALNRVD 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALGKIKIER 240
           DVLST+EINARSTFKV DS AVKFRWSM FPT MKK+EFTAK PLSKMPYLALGKIKIER
Sbjct: 181 DVLSTMEINARSTFKVGDSTAVKFRWSMTFPTCMKKDEFTAKAPLSKMPYLALGKIKIER 240

Query: 241 AAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAPATPPVE 300
           AAAS+ ERE+N+  GAGEFS LKKHL+DLW ESRWLKKNIEQLQSEI +QKAAP+TPPVE
Sbjct: 241 AAASESERESNKAAGAGEFSGLKKHLEDLWKESRWLKKNIEQLQSEIGEQKAAPSTPPVE 300

Query: 301 TRKKKG 307
           TRKKKG
Sbjct: 301 TRKKKG 306

BLAST of Lsi03G012320 vs. NCBI nr
Match: XP_023526002.1 (uncharacterized protein LOC111789609 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 455.3 bits (1170), Expect = 4.2e-124
Identity = 236/311 (75.88%), Postives = 264/311 (84.89%), Query Frame = 0

Query: 1   MKASIVFREDQ-NPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYR 60
           MKASI+FREDQ NP FRAKIPLNFFGFPFRSAI+LAE DNLSFTF +FFRSGP+FN SYR
Sbjct: 1   MKASIIFREDQRNPTFRAKIPLNFFGFPFRSAIQLAEPDNLSFTFTSFFRSGPAFNFSYR 60

Query: 61  PNHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSV 120
           PN SL PF+LAVKAGIGL+GS IDSPM FTAEFNLPGN+PPRFFLHFRP+LGDFTLRRSV
Sbjct: 61  PNDSLAPFTLAVKAGIGLYGSSIDSPMNFTAEFNLPGNKPPRFFLHFRPRLGDFTLRRSV 120

Query: 121 QSHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETS-----DLGLGHPVLSIQRIDCSE 180
           QSH A+FNLP       +DDD+AA+S GKSVDGGE+S     DLGLG+ +LS QRI CS 
Sbjct: 121 QSHTASFNLP-------LDDDLAAMSSGKSVDGGESSGEVHADLGLGNTILSGQRIHCSG 180

Query: 181 VLNRVDDVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAKLPLSKMPYLALG 240
           + NR  D+LST EINARSTFKV++SA+VKF+W MRFP SMKKEEFTAK+ LSK+PYLAL 
Sbjct: 181 IFNRFHDLLSTGEINARSTFKVKNSASVKFQWCMRFPMSMKKEEFTAKVMLSKLPYLALR 240

Query: 241 KIKIERAAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAAP 300
           KIKIE A ASD ERE+NE   AGE S LKKHLDDL TESRW+KKNIEQL+SEI DQ AAP
Sbjct: 241 KIKIELAKASDSERESNEAVSAGEISVLKKHLDDLRTESRWMKKNIEQLRSEIGDQMAAP 300

Query: 301 ATPPVETRKKK 306
           A PPVE+RKK+
Sbjct: 301 ANPPVESRKKR 304

BLAST of Lsi03G012320 vs. NCBI nr
Match: KAG6581601.1 (hypothetical protein SDJN03_21603, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 453.4 bits (1165), Expect = 1.6e-123
Identity = 239/312 (76.60%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MKASIVFREDQ-NPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYR 60
           MKASI+FREDQ NPIFRAKIPLNFFGFPFRSAI+LAE DNLSFTF +FFRSGPSFN SYR
Sbjct: 1   MKASIIFREDQRNPIFRAKIPLNFFGFPFRSAIQLAEPDNLSFTFTSFFRSGPSFNFSYR 60

Query: 61  PNHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSV 120
           PN SL PF+LA+KAGIGL+GS IDSPM FTAEFNLPGN+PPRFFLHFRP+LGDFTLRRSV
Sbjct: 61  PNDSLAPFTLALKAGIGLYGSSIDSPMNFTAEFNLPGNKPPRFFLHFRPRLGDFTLRRSV 120

Query: 121 QSHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETS-----DLGLGHPVLSIQRIDCSE 180
           QSH A+FNLP       +DDD+AA+S GKSVD GE+S     DLGLG+ +LS QRI CS 
Sbjct: 121 QSHTASFNLP-------LDDDLAAMSSGKSVDSGESSGEVHTDLGLGNTMLSGQRIHCSG 180

Query: 181 VLNRVDDVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKEEFTAK-LPLSKMPYLAL 240
           + NR  D+LST EINARSTFKV++SAAVKF+W MRFP SMKKEEFTAK + LSK+PYLAL
Sbjct: 181 IFNRFHDLLSTGEINARSTFKVKNSAAVKFQWCMRFPMSMKKEEFTAKVMLLSKLPYLAL 240

Query: 241 GKIKIERAAASDGERETNETGGAGEFSALKKHLDDLWTESRWLKKNIEQLQSEISDQKAA 300
            KIKIE AAASD ERE+NE   AGE S LKKHLDDL TESRW+KKNIEQL+SEI DQ AA
Sbjct: 241 RKIKIELAAASDSERESNEAVSAGEISVLKKHLDDLRTESRWMKKNIEQLRSEIGDQVAA 300

Query: 301 PATPPVETRKKK 306
           PA PPVE+RKKK
Sbjct: 301 PANPPVESRKKK 305

BLAST of Lsi03G012320 vs. TAIR 10
Match: AT3G57990.1 (unknown protein; Has 1497 Blast hits to 1323 proteins in 52 species: Archae - 0; Bacteria - 4; Metazoa - 23; Fungi - 34; Plants - 61; Viruses - 0; Other Eukaryotes - 1375 (source: NCBI BLink). )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 122/307 (39.74%), Postives = 177/307 (57.65%), Query Frame = 0

Query: 1   MKASIVFREDQNPIFRAKIPLNFFGFPFRSAIELAEADNLSFTFNTFFRSGPSFNLSYRP 60
           MKAS+ FRE+Q P+FRAK+PL+  G PF+S I   E+  LS   +TFF SGPS  ++YRP
Sbjct: 1   MKASMKFREEQKPLFRAKVPLSILGLPFQSGIVAGESKELSLNLSTFFESGPSLKVAYRP 60

Query: 61  NHSLNPFSLAVKAGIGLFGSPIDSPMTFTAEFNLPGNQPPRFFLHFRPQLGDFTLRRSVQ 120
           N S NPFSL VK G G FGSPI S M  +AEFNL G   P F LHF+PQ GDF++++S  
Sbjct: 61  NDSWNPFSLIVKTGSGSFGSPISSSMLMSAEFNLLGQGNPSFMLHFKPQFGDFSIKKSHS 120

Query: 121 SHIANFNLPSRNSISQVDDDVAALSRGKSVDGGETSDLGLGHPVLSIQRIDCSEVLNRVD 180
           S     NL    + S  +DD    S  + VD    +  G G   +++     S     + 
Sbjct: 121 SSGFERNLIKSMNGSVSEDD----SSIEVVDTPAVNGCGGGFRKVTVLP---STSAGDIA 180

Query: 181 DVLSTVEINARSTFKVRDSAAVKFRWSMRFPTSMKKE-EFTAKLPLSKMPYLALGKIKIE 240
            +LS VE+ AR++  VR  A + FRW +R PT ++++ + TA + L + P+L + KI IE
Sbjct: 181 GLLSGVEVAARTSLPVRGRAVLNFRWGVRVPTEIRRDFDPTAAISLRRFPFLVMNKIGIE 240

Query: 241 RAAASDGERETNETGGAGEFSA---------LKKHLDDLWTESRWLKKNIEQLQSEISDQ 297
               +D  + T  TG  G+ S          + + +++L TE++ LK+ +E L+  IS+ 
Sbjct: 241 HVDGADA-KVTKSTGDPGKVSGPAQFTTSGDVAEVIEELRTENKQLKRAVEDLREVISNV 299